CN202916237U - Electrode loaded with plurality of layers of glucose dehydrogenase magnetic nanometer microspheres - Google Patents
Electrode loaded with plurality of layers of glucose dehydrogenase magnetic nanometer microspheres Download PDFInfo
- Publication number
- CN202916237U CN202916237U CN 201220340692 CN201220340692U CN202916237U CN 202916237 U CN202916237 U CN 202916237U CN 201220340692 CN201220340692 CN 201220340692 CN 201220340692 U CN201220340692 U CN 201220340692U CN 202916237 U CN202916237 U CN 202916237U
- Authority
- CN
- China
- Prior art keywords
- electrode
- nano microsphere
- nanometer
- layer
- reaction
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Landscapes
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses an electrode loaded with a plurality of layers of glucose dehydrogenase magnetic nanometer microspheres. The electrode is provided with a substrate, an electrode, a first nanometer microsphere layer and a second nanometer microsphere layer in sequence, wherein the substrate is arranged in the lowermost position of the electrode; the electrode is arranged on the upper surface of the substrate; the first nanometer microsphere layer is coated on the upper surface of the electrode; and the second nanometer microsphere layer is mutually adsorbed with the first nanometer microsphere layer through magnetic force. The electrode has the following advantages that firstly, the nanometer surface is spherical, and the specific surface area of a nanometer particle reaction system with an enzyme layer is far greater than that of a plane structure, so that more reaction enzyme can be loaded within the unit volume, the reaction efficiency is higher, and the reaction strength is greater; secondly, through a nanometer particle packing structure formed by self assembly, the glucose response within an area is more complete, and the bioelectric current with greater strength is generated; and thirdly, through a nanometer particle system with the packing structure, the reaction time is more durable when compared with the reaction time in a traditional method, and greater and more durable current can be provided, so that the current detection precision is higher.
Description
Technical field
The utility model relates to a kind of medical detecting electrode, and relating in particular to a kind of load has multilayer glucose dehydrogenase magnetic Nano microsphere, by detecting sugar concentration in the saliva and then obtaining the electrode of blood sugar for human body data.
Background technology
The blood sugar test process is converted into the basic process of electric signal by chemical reaction, principle is (such as saliva by the reactant liquor that contains glucose molecule, the body fluid such as blood) at the glucose response enzyme (such as glucose dehydrogenase, glucose oxidase) carries out the chemolysis reaction under the catalysis, thereby discharge charged particle (such as hydroxide ion, hydrogen ion and electronics), charged particle is transferred to electric signal detection system by the wire of electrode and connecting electrode with charged particle, judge response intensity according to the size of current that produces, response intensity greatly then reacts acutely, and glucose content is just high; Vice versa.
In the prior art, the enzyme layer mainly adopts the way that directly is coated with reactant liquor on electrode, namely directly the glucose response enzyme solutions is coated in electrode surface, for improving the adhesive strength of reaction enzymes, generally be that saturated glucose response enzyme solutions is made the solution with certain viscosity that is easy to be coated with, utilize the high-speed rotary automatic doubler surface glouer, carry out repeatedly spray-type spin coating at electrode surface.Or utilize the mode of serigraphy, repeatedly be coated with at electrode surface.
No matter be spin coating or serigraphy, its electrode modification all needs to carry out multi-pass operations, can't finish with a procedure is disposable.How high glucose response enzyme solutions no matter its solubleness have, and reaction enzymes all is irregular being dispersed in the solution, therefore be applied to the uneven distribution that will form reaction enzymes after the electrode surface, and the enzyme content of each distributed points is lower.
The glucose response enzyme is difficult to form stacked structure at electrode surface, namely only have a small amount of reaction enzymes to exist at a certain reflecting point, in the breakdown of glucose reaction, be easy to be consumed totally, the reaction of this point carry out complete after, although the local low concentration region that has formed glucose, glucose molecule can be to this reaction zone infiltration, because reaction enzymes does not have compensability, changing the time does not have reaction enzymes, and therefore reaction does not have continuation.Cause the electric current that produces little, and the duration is short, is difficult to detect and judges.
The utility model content
The utility model is for above problem, and a kind of load that proposes has the electrode of multilayer glucose dehydrogenase magnetic Nano microsphere, has sandwich construction:
Be positioned at electrode the below, play the substrate of support effect;
Be covered in the electrode of described substrate top surface;
Use czochralski method to be coated in the first Nano microsphere layer of described electrode top, it has the Nano microsphere of individual layer;
Be covered in described ground floor Nano microsphere top, by the second Nano microsphere layer that magnetic force and ground floor Nano microsphere adsorb mutually, it has the Nano microsphere that multilayer attracts each other and piles up by magnetic force.
Has the space of passing through for glucose molecule between each Nano microsphere of the first Nano microsphere layer.
Has the space of passing through for glucose molecule between each microballoon in the second Nano microsphere layer.The area load of Nano microsphere has glucose molecule.
Because the load of having adopted technique scheme, the utility model to provide has the electrode of the magnetic Nano microsphere of glucose dehydrogenase, has following advantage:
1. reaction efficiency improves, because Nanosurface is spherical, specific surface area is much larger than planar structure with the nano particle reaction system of enzyme layer, can the more reaction enzymes of load in the unit volume, so that reaction efficiency is higher, response intensity is larger.
2. the nano particle packing structure that forms of self assembly, so that glucose response is more thorough in the zone, the bioelectric current of the greater strength that can produce.
3. the nanoparticle system of packing structure so that the reaction time is more lasting than classic method, can provide larger, more lasting electric current, so that current detection accuracy is higher.
4. owing to larger electric current, the time of generation current is more lasting, so present technique can be for detection of the lower glucose solution of concentration.Only can be applied to blood such as the traditional detection technology, present technique can be used for concentration of glucose far below the saliva of blood (saliva sugar content only for blood-sugar content 1%) in the detection of glucose content.
Description of drawings
Technical scheme for clearer explanation embodiment of the present utility model or prior art, the below will do one to the accompanying drawing of required use in embodiment or the description of the Prior Art and introduce simply, apparently, accompanying drawing in the following describes only is embodiment more of the present utility model, for those of ordinary skills, under the prerequisite of not paying creative work, can also obtain according to these accompanying drawings other accompanying drawing.
Fig. 1 is structural representation of the present utility model
Fig. 2 is the SEM image of the utility model electrode surface Nano microsphere packing structure
Fig. 3 is the utility model electrode reaction system synoptic diagram
Embodiment
For the purpose, technical scheme and the advantage that make embodiment of the present utility model is clearer, below in conjunction with the accompanying drawing among the utility model embodiment, the technical scheme among the utility model embodiment is known complete description:
As shown in Figure 1: a kind of load has the electrode of multilayer glucose dehydrogenase magnetic Nano microsphere, has the structure that multilayer is piled up, and has successively: be positioned at electrode the below, play the substrate 4 of support effect.Upper surface at substrate 4 is coated with electrode 3.Upper surface at electrode 3 is coated with the first Nano microsphere layer 1 by czochralski method, and described the first Nano microsphere layer 1 has single layer structure, and a plurality of Nano microspheres are coated on the electrode uniformly, the surface load glucose dehydrogenase of each Nano microsphere.Leave the gap that to pass through for glucose molecule between the described Nano microsphere.
Above the first Nano microsphere layer 1, be coated with the second Nano microsphere layer 2, as a better embodiment: will be by described substrate 4, the coating base that electrode 3 and the first Nano microsphere layer 1 are made vertically immerses and fills the reactor that contains the Nano microsphere mixed solution, apply the magnetic field of a level to reactor, after reaction a period of time, self assembly by magnetic Nano microsphere, can the upper surface of described the first Nano microsphere layer 1 form one stable, the second Nano microsphere layer 2 with multi-layer nano microballoon packing structure, same, Nano microsphere in the second Nano microsphere layer 2 all load has glucose molecule, leaves the space that can pass through for glucose molecule between the Nano microsphere.
Have shown in Figure 3: have the actual reflection system of electrode of the present utility model as follows: at first react by being under this regional glucose catalysis in reaction enzymes in a certain zone, discharge charged particle.Because this zone of reaction forms the low concentration region of glucose, glucose in the reactant liquor constantly spreads to low concentration region owing to diffusion principle, after exhausting the total overall reaction enzyme of the first floor, can permeate to lower floor by the slit (the glucose molecule size is former in nanoparticle size) of piling up particulate, under the catalysis of lower floor's nano grain surface reaction enzymes, continue reaction, again form low concentration region, thereby constantly repeat the reaction of realization in the first floor conversion zone, until orlop.This reaction system is much better than the electrode of individual layer enzymatic structure in the conventional art, can improve to greatest extent reaction efficiency, and improves continual and steady electric current output.
The above; it only is the better embodiment of the utility model; but protection domain of the present utility model is not limited to this; anyly be familiar with those skilled in the art in the technical scope that the utility model discloses; be equal to replacement or change according to the technical solution of the utility model and utility model design thereof, all should be encompassed within the protection domain of the present utility model.
Claims (4)
1. a load has the electrode of multilayer glucose dehydrogenase magnetic Nano microsphere, has the packing structure of multi-layer nano microballoon, comprising:
Be positioned at electrode the below, play the substrate of support effect;
Be covered in the electrode of described substrate top surface;
Use czochralski method to be coated in the first Nano microsphere layer of described electrode top, it has the Nano microsphere of individual layer;
Be covered in described ground floor Nano microsphere top, the Nano microsphere that by the second Nano microsphere layer that magnetic force and ground floor Nano microsphere adsorb mutually, it has at many levels, attract each other and pile up by magnetic force.
2. load according to claim 1 has the electrode of multilayer glucose dehydrogenase magnetic Nano microsphere, is further characterized in that: have the space of passing through for glucose molecule between each Nano microsphere of described the first Nano microsphere layer.
3. load according to claim 2 has the electrode of multilayer glucose dehydrogenase magnetic Nano microsphere, is further characterized in that: have the space of passing through for glucose molecule between each microballoon in described the second Nano microsphere layer.
4. the described load of any one claim has the electrode of multilayer glucose dehydrogenase magnetic Nano microsphere according to claim 1-3, and be further characterized in that: the area load of described Nano microsphere has glucose molecule.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220340692 CN202916237U (en) | 2012-07-13 | 2012-07-13 | Electrode loaded with plurality of layers of glucose dehydrogenase magnetic nanometer microspheres |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220340692 CN202916237U (en) | 2012-07-13 | 2012-07-13 | Electrode loaded with plurality of layers of glucose dehydrogenase magnetic nanometer microspheres |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202916237U true CN202916237U (en) | 2013-05-01 |
Family
ID=48164541
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201220340692 Expired - Fee Related CN202916237U (en) | 2012-07-13 | 2012-07-13 | Electrode loaded with plurality of layers of glucose dehydrogenase magnetic nanometer microspheres |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202916237U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP7068327B2 (en) | 2017-03-03 | 2022-05-16 | シーメンス・ヘルスケア・ダイアグノスティックス・インコーポレーテッド | Manufacturing method of biosensor containing nanobeads and its use |
-
2012
- 2012-07-13 CN CN 201220340692 patent/CN202916237U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP7068327B2 (en) | 2017-03-03 | 2022-05-16 | シーメンス・ヘルスケア・ダイアグノスティックス・インコーポレーテッド | Manufacturing method of biosensor containing nanobeads and its use |
| US11585809B2 (en) | 2017-03-03 | 2023-02-21 | Siemens Healthcare Diagnostics Inc. | Nanobead containing biosensors and methods of production and use thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105633425B (en) | A kind of Pdx@Pt/C nucleocapsid fuel battery cathod catalysts and preparation method thereof | |
| Lim et al. | Nanostructured sol-gel electrodes for biofuel cells | |
| CN103852512B (en) | A kind of electrochemical sensor and preparation method being detected hydrogen oxide | |
| Li et al. | Pd nanoclusters confined in ZIF-8 matrixes for fluorescent detection of glucose and cholesterol | |
| CN107037102B (en) | Nano composite material and preparation method and application thereof | |
| CN103336043B (en) | Preparation method of hydrogen peroxide biosensor | |
| CN103344689B (en) | Based on Fe 3o 4magnetic pole, the preparations and applicatio of-AuNPs magnetic composite nanoparticles immobilization acetylcholinesteraseelectrochemistry | |
| CN103913492A (en) | Keggin type heteropoly acid-polypyrrole-graphene composite material modified electrode as well as preparation method and application thereof | |
| CN105958059A (en) | Three-dimensional Cu/Co3O4 multi-stage nanowire array and preparation method and application thereof | |
| CN107037104A (en) | It is a kind of based on the protein electrochemistry senser element of modified by graphene quantum dot electrode and its preparation and application | |
| CN107941889A (en) | A kind of preparation and electrochemical sensing application study of stannic oxide three-dimensional grapheme nanocomposite fixing protein modified electrode | |
| CN103066302B (en) | A kind of biological fuel cell anode and preparation method thereof and application | |
| CN106226373A (en) | The preparation method of a kind of gold/titanium dioxide/carbon nano tube sensor and the method for synchronous detecting biological micromolecule thereof | |
| CN103575781A (en) | Electrochemical sensor and its preparation method | |
| CN202916237U (en) | Electrode loaded with plurality of layers of glucose dehydrogenase magnetic nanometer microspheres | |
| CN101545886B (en) | Composite sensing membrane preparation method with nano gold evenly distributed in conducting polymer | |
| CN102590308B (en) | Porous biosensor and making and application methods | |
| CN202916235U (en) | Electrode loaded with plurality of layers of hexokinase magnetic nanometer microspheres | |
| CN202916236U (en) | Electrode loaded with plurality of layers of glucose oxidase magnetic nanometer microspheres | |
| CN110568050B (en) | Preparation method of enzyme-free catalytic hydrogen peroxide electrochemical sensor based on flexible electrode | |
| CN104132982B (en) | Dehydrogenase electrode and preparation method thereof | |
| CN103506161A (en) | Graphene composite, application of graphene composite to catalytic methanol oxidation, chemically modified electrode and preparation method of graphene composite | |
| CN103743801A (en) | Droplet-microfluidic-based preparation method of platinum black-modified electrode biosensor and application thereof | |
| CN102830147B (en) | Modified electrode and applications of modified electrode on detecting micro/trace nitro aromatic compound | |
| Neetzel et al. | Facile wet-chemical synthesis of differently shaped cuprous oxide particles and a thin film: Effect of catalyst morphology on the glucose sensing performance |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130501 Termination date: 20160713 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |