CN202735314U - 可变波长的紫外及可见分光检测器 - Google Patents
可变波长的紫外及可见分光检测器 Download PDFInfo
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Abstract
本实用新型公开了可变波长的紫外及可见分光检测器,其特征在于:在光源输出的主光路上,依次设置有组合光源、狭缝、滤光片、凹面镜、罗兰光栅、可见光分光镜、比色皿、检验池和液晶显示系统;所述的组合光源为氘灯和钨灯的组合光源。所述的可见光分光镜上连接有光电二极管。本实用新型的检测器不但安装有紫外吸收检测器,可检测蛋白质、核酸、氨基酸、核苷酸、多肽、激素等有机物质;还设置有可见光检测器,同时也能检测金属离子,大大拓宽了检测器的检测面。同时,配合氘灯和钨灯的组合光源,也大大增加了光源的波段,增加检测的范围和提高了检测的精确度。
Description
技术领域
本实用新型涉及色谱分析检测仪器技术领域,特别是指可变波长的紫外及可见分光检测器。
背景技术
高效液相色谱法是在经典色谱法的基础上,引用了气相色谱的理论,在技术上,流动相改为高压输送(最高输送压力可达3.5万KPa);色谱柱是以特殊的方法用小粒径的填料填充而成,从而使柱效大大高于经典液相色谱(每米塔板数可达几万或几十万);同时柱后连有高灵敏度的检测器,可对流出物进行连续检测。高效液相色谱常用的检测器有紫外检测器、示差折光检测器和荧光检测器三种。紫外检测器适用于对紫外光(或可见光)有吸收性能样品的检测。其特点是使用面广(如蛋白质、核酸、氨基酸、核苷酸、多肽、激素等均可使用);灵敏度高(检测下限为10-10g/ml);线性范围宽;对温度和流速变化不敏感;可检测梯度溶液洗脱的样品,但是对某些金属离子配合物的检测效能并不好。示差折光检测器主要用于具有与流动相折光率不同的样品组分的检测,目前,糖类化合物的检测大多使用此检测系统。这一系统通用性强、操作简单,但灵敏度低(检测下限为10-7g/ml),流动相的变化会引起折光率的变化,因此,它既不适用于痕量分析,也不适用于梯度洗脱样品的检测。荧光检测器只适用于具有荧光的有机化合物(如多环芳烃、氨基酸、胺类、维生素和某些蛋白质等)的测定,其灵敏度很高(检测下限为10-12~10-14g/ml),痕量分析和梯度洗脱作品的检测均可采用。另外,在检测器光源设备上,也存在波长范围窄等不足。
发明内容
本实用新型的目的在于提供一种光源波段宽、并能检测有机物质、某些无机物质以及金属离子等的可变波长的紫外及可见分光检测器。
为实现本实用新型的目的,拟采用以下技术方案:
可变波长的紫外及可见分光检测器,其特征在于:在光源输出的主光路上,依次设置有组合光源、狭缝、滤光片、凹面镜、罗兰光栅、可见光分光镜、比色皿、检验池和液晶显示系统;所述的组合光源为氘灯和钨灯的组合光源。
作为优选,所述的可见光分光镜上连接有光电二极管。
与现有技术相比,本实用新型的检测器不但安装有紫外吸收检测器,可检测蛋白质、核酸、氨基酸、核苷酸、多肽、激素等有机物质;还设置有可见光检测器,同时也能检测金属离子,大大拓宽了检测器的检测面。同时,配合氘灯和钨灯的组合光源,也大大增加了光源的波段,增加检测的范围和提高了检测的精确度。
附图说明
图1为本实用新型实施例的光路图。
图中的标号为:1、组合光源;2、狭缝;3、滤光片;4、凹面镜;5、罗兰光栅;6、可见光分光镜;7、比色皿;8、检验池;9、液晶显示系统;10、光电二极管。
具体实施方式
下面结合附图及实施例来进一步说明本实用新型的技术方案。
图1示意了本实用新型的光路图,本实用新型实施例在组合光源1输出的主光路上,依次设置有狭缝2、滤光片3、凹面镜4、罗兰光栅5、可见光分光镜6、比色皿7、检验池8和液晶显示系统9,所述的可见光分光镜5上连接有光电二极管10,其中的组合光源1为氘灯和钨灯的组合光源,也大大增加了光源的波段,增加检测的范围和提高了检测的精确度。本实用新型的检测器不但安装有紫外吸收检测器,可检测蛋白质、核酸、氨基酸、核苷酸、多肽、激素等有机物质;还设置有可见光检测器,同时也能检测金属离子,大大拓宽了检测器的检测面。
以上显示和描述了本实用新型的基本原理、主要特征和本实用新型的优点。本行业的技术人员应该了解,本实用新型不受上述实施例的限制,上述实施例和说明书中描述的只是说明本实用新型的原理,在不脱离本实用新型精神和范围的前提下本实用新型还会有各种变化和改进,这些变化和改进都落入要求保护的本实用新型范围内。本实用新型要求保护范围由所附的权利要求书及其等同物界定。
Claims (2)
1.可变波长的紫外及可见分光检测器,其特征在于:在光源输出的主光路上,依次设置有组合光源、狭缝、滤光片、凹面镜、罗兰光栅、可见光分光镜、比色皿、检验池和液晶显示系统;所述的组合光源为氘灯和钨灯的组合光源。
2.根据权利要求1所述的可变波长的紫外及可见分光检测器,其特征在于:所述的可见光分光镜上连接有光电二极管。
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CN106018309A (zh) * | 2016-05-05 | 2016-10-12 | 武汉光昱科技有限公司 | 一种多功能核酸蛋白检测系统 |
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CN106018309A (zh) * | 2016-05-05 | 2016-10-12 | 武汉光昱科技有限公司 | 一种多功能核酸蛋白检测系统 |
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