CN202649215U - Allergen specific antibody immunoglobulin G (IgG) immunoblotting detection kit - Google Patents
Allergen specific antibody immunoglobulin G (IgG) immunoblotting detection kit Download PDFInfo
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- CN202649215U CN202649215U CN 201220246533 CN201220246533U CN202649215U CN 202649215 U CN202649215 U CN 202649215U CN 201220246533 CN201220246533 CN 201220246533 CN 201220246533 U CN201220246533 U CN 201220246533U CN 202649215 U CN202649215 U CN 202649215U
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Abstract
The utility model provides an allergen specific antibody immunoglobulin G (IgG) immunoblotting detection kit which is characterized by comprising a test strip and a reagent bottle. The test strip comprises a test paper body and a reaction tank, and the test paper body is arranged at the bottom of the reaction tank. The allergen specific antibody (IgG) immunoblotting detection kit has the advantage that the nitrocellulose membrane test strip is arranged at the bottom of the reaction tank, a plurality of allergen proteins are covered on the nitrocellulose membrane test strip, other reaction and detection reagents are further contained in the kit, a blood serum sample of a detector is added in the reaction tank, variety and degree of food allergens of the detector can be detected conveniently, and the allergen specific antibody (IgG) immunoblotting detection kit is high in sensitivity and convenient and safe to use.
Description
Technical field
The utility model relates to a kind of food allergen and detects box, relates in particular to a kind of sxemiquantitative or quantitatively detects the immune marking (Western Blot) detection kit of Food intolerance specific IgG in the human serum.
Background technology
Anaphylactia is modal a kind of disease in the modern society, and its pathogenesis is that the tissue damage or the physiological function that cause after body is stimulated by antigenic substance (also claiming anaphylactogen allergen or allergen) are disorderly.
Food intolerance is a kind of of anaphylactia; it is complicated allergic disease; pathology mechanism is: people's immune system is objectionable impurities entering that certain or multiple food in the body is used as; thus produce excessive protective immunological reaction for these materials, produce food specific IgG antibody.
Food should digestedly be decomposed into amino acid, glycerine and monose in theory after entering alimentary canal, then be converted into energy or tissue and consist of.But true really not so, a lot of foods (comprise us the most normal some edible food) are in default of corresponding enzyme and can't be by the human body catapepsis, thereby enter enteron aisle with polypeptide or other form, some of them can be produced immune response as foreign matter identification by body, generate at last to have the specific IgG antibody of food.When this IgG antibody when food particle contacts again, it will form immune complex (III allergic reaction type) with food particle, and will be used as refuse by body and discharge from kidney.And not all immune complex can both pass through the glomerulus filter membrane, and the immune complex that can't filter then can stop up the filtration structure of kidney, causes the glomerular filtration voltage rise high, then causes blood pressure rising, vascular wall expansion and cholesterol deposition.Human body waste liquid (especially adipocyte) can not normally retention finally can cause oedema and obesity in tissue by the kidney discharge.If untimely change dietary structure, the food that does not tolerate can continue to form compound, increases the weight of original symptom and constantly lasting.Thereby cause the immune system overload, cause each system of human body to occur such as series of symptoms diseases such as hypertension, obesity, headache or antimigraine, chronic diarrhea, fatigue, infection.
Determine at present the main intracutaneous test of method and the pricking method test of anaphylactogen, the method can obtain testing result general half an hour, has easy, quick, inexpensive advantage.But defective is that false positive is high and be subjected to the impact of other drug larger, test to take Claritin in front 24 hours and can affect measurement result, even the patient of severe allergic reaction makes a skin test and also has certain life danger.
The kind that detects anaphylactogen by experiment in vitro can certainly be arranged, as passing through to extract a small amount of serum, the content of the specific IgG that then in the test sample some anaphylactogen is produced is finished the detection of anaphylactogen screening and specific allergen, the very inconvenience but the method often operates.
The utility model content
In view of the deficiencies in the prior art, the utility model first aspect provides a kind of highly sensitive, easy to use, safe allergen specificity antibody IgG Western blot detection kit.
The utility model provides following technical scheme:
A kind of allergen specificity antibody IgG Western blot detection kit, it is characterized in that: comprise test strips and reagent bottle, described test strips comprises test paper body and reactive tank, and the test paper body is arranged at reaction tank bottom.
Described reagent bottle can be the anti-human reagent bottle of biotin labeled IgG, Avidin-horseradish peroxidase reagent bottle, tmb substrate colour developing bottle and sample dilution bottle wherein one or more.
The beneficial effects of the utility model: because the reactive tank inner bottom part is established the nitrocellulose membrane test strips, this nitrocellulose membrane test strips is had several allergen protein, also be contained with other reactions in the kit and detect reagent, add tester's serum sample in reactive tank, can detect easily kind and the degree of tester's food allergen, highly sensitive, easy to use, safe.
Description of drawings
The utility model is described in further detail below in conjunction with drawings and Examples, in the accompanying drawing:
Fig. 1 is reactive tank structural representation in the utility model.
Embodiment
In order to make the purpose of this utility model, technical scheme and advantage clearer, below in conjunction with embodiment and accompanying drawing, the utility model is further described.
See also accompanying drawing 1, the utility model provides a kind of allergen specificity antibody IgG Western blot detection kit, can be used for human body food hypersenstivity antigen is detected, comprise that the nitrocellulose membrane of allergen protein test strips 2 and reactive tank 1 are arranged, described test strips is positioned at the bottom of reactive tank 1.
Described reagent bottle can be the anti-human reagent bottle 3 of biotin labeled IgG, Avidin-horseradish peroxidase reagent bottle 4, tmb substrate colour developing bottle 5 and sample dilution bottle 6 wherein one or more.
The utility model is because reactive tank 1 inner bottom part is provided with nitrocellulose membrane test strips 2, this nitrocellulose membrane test strips 2 is had several allergen protein, also be contained with other reactions in the kit and detect reagent, add tester's serum sample in reactive tank 1, can detect easily kind and the degree of tester's food allergen, highly sensitive, easy to use, safe.
The above only is preferred embodiment of the present utility model; not in order to limit the utility model; all within spirit of the present utility model and principle, any modification of doing, be equal to replacement, improvement etc., all should be included within the protection domain of utility model.
Claims (2)
1. allergen specificity antibody IgG Western blot detection kit, it is characterized in that: comprise test strips and reagent bottle, described test strips comprises test paper body and reactive tank, and the test paper body is arranged at reaction tank bottom.
2. kit according to claim 1 is characterized in that: described reagent bottle can be the anti-human reagent bottle of biotin labeled IgG, Avidin-horseradish peroxidase reagent bottle, tmb substrate colour developing bottle and sample dilution bottle wherein one or more.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220246533 CN202649215U (en) | 2012-05-28 | 2012-05-28 | Allergen specific antibody immunoglobulin G (IgG) immunoblotting detection kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220246533 CN202649215U (en) | 2012-05-28 | 2012-05-28 | Allergen specific antibody immunoglobulin G (IgG) immunoblotting detection kit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202649215U true CN202649215U (en) | 2013-01-02 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201220246533 Expired - Fee Related CN202649215U (en) | 2012-05-28 | 2012-05-28 | Allergen specific antibody immunoglobulin G (IgG) immunoblotting detection kit |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202649215U (en) |
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2012
- 2012-05-28 CN CN 201220246533 patent/CN202649215U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130102 Termination date: 20130528 |