CN202626186U

CN202626186U - Creatinine determination kit

Info

Publication number: CN202626186U
Application number: CN 201220157044
Authority: CN
Inventors: 许国和; 胡露群
Original assignee: PUREBIO LABORATORIES (NINGBO) Ltd
Current assignee: Ningbo Purui Bai biotechnology Limited by Share Ltd
Priority date: 2012-04-13
Filing date: 2012-04-13
Publication date: 2012-12-26
Anticipated expiration: 2022-04-13

Abstract

The utility model relates to a creatinine determination kit, which is characterized by comprising a box body, a box cap capable of opening and closing the box body, and four first reagent bottles and four second reagent bottles which are arranged in the box body in parallel, and each first reagent bottle comprises a flat first bottle and a first bottle cap; the upper end surface of each first bottle body is convexly provided with a first threaded port matched with each first bottle cap, and each second reagent bottle comprises a cylindrical second bottle body and a second bottle cap; and the upper end surface of each second bottle body is convexly provided with a second threaded port matched with each second bottle cap. Compared with the prior art, the creatinine determination kit provided by the utility model has the advantages that the reagent bottles are combined simply, the operation steps are simplified, the flexibility is high, the determination results are accurate, the kit is not polluted by endogenous and exogenous matters, and meanwhile, the storage and transportation are convenient, and the creatinine determination kit is suitable for rapid determination.

Description

The creatinine assay test kit

Technical field

The utility model relates to a kind of creatinine assay test kit, belongs to medical detection technology.

Background technology

Creatine concentration can be used as some resolvents (BSA, AMS) excretory reference substance in the urine, in medical treatment, has clinical meaning.Measure creatinine and mainly contain chemical assay (Jaffe method), enzyme process, high performance liquid chromatography (HPLC) and capillary electrophoresis etc.

Chemical assay is with low cost one by one, and is easy and simple to handle, is the TNP creatinine mixture that creatinine and picrate effect in a bite sample of the most frequently used method of present domestic mensuration creatinine generate yellowish red color.The shortcoming of this method is that specificity is not high, because vitamins C, acetone, etheric acid, methyldopa and high concentration glucose, protein and some microbiotic such as penicillin G, cefoxitin, Kefzol etc. also can generate red with the alkaline picric acid reaction.

High performance liquid chromatography (HPLC) (HPLC) creatinine one by one is positively charged in weak acid environment, through the cation-exchange chromatography post can with other composition well separated, measure its photoabsorption at 234nm.The precision that this method is analyzed is high, specificity is good, but this law is inappropriate for clinical samples analysis in enormous quantities, usually only as the reference method of creatinine assay, is used to estimate the test kit and some scientific research purpose of commercially available creatinine assay.

Capillary electrophoresis serum specimen is one by one done pre-treatment with high speed centrifugation, and urine specimen can be used low-speed centrifugal, removes formed elements, and supernatant is measured 235nm place absorbancy after moving the electrocapillary electrophoretic separation with micella.It is wide that this law is measured linearity range, operate comparatively easy, but need with specific installation with carry out the pre-treatment of serum specimen, routine clinical use is difficult.

The enzymatic determination method mainly contains Creatinine deiminase (Creatinine deiminase) and Creatininase (Creatininase) two big classes one by one.The patent No. is that the Chinese invention patent " nicotinamide coenzyme reagent and method for making thereof " of ZL02139298.6 discloses the mensuration reagent of using the preparation of enzyme reaction product oxidized form nicotinamide coenzyme.But this patent has following shortcoming: first; This system is in the needed reduced form nicotinoyl of formation reaction ammonia coenzyme; Also offset the activity of part alanine aminotransferase, aspartate amino transferase, urea, ammonia, creatinine and carbonic acid gas etc., caused the accuracy of reagent test to reduce greatly; The second, this reagent must react for some time before official testing in advance, so that can produce enough reduced form nicotinoyl ammonia coenzyme, brings very big inconvenience to test.

The utility model content

The utility model technical problem to be solved is to provide a kind of operation steps simple creatinine assay test kit to the above-mentioned state of the art.

The utility model solves the problems of the technologies described above the technical scheme that is adopted: a kind of creatinine assay test kit; It is characterized in that this test kit comprises a box body, can open and close the lid of box body and is positioned over intravital four first reagent bottles of box and four second reagent bottles side by side; Further; Described first reagent bottle comprises first bottle and first bottle cap that is flat-shaped; The aforesaid first bottle upper surface is convexly equipped with and is formed with first threaded port, and described second reagent bottle comprises second bottle and second bottle cap that is column, and the aforesaid second bottle upper surface is convexly equipped with and is formed with second threaded port.

For ease of identification, the outside surface of said box body is pasted with barcode and indicates.

Good ' the damping fluid of 50mmol/L is housed, the E.C. 3.5.3.3 of >=8ku/L, the sarcosine oxidase of >=8ku/L and the TOOS of 0.4mmol/L in first reagent bottle; Good ' the damping fluid of 50mmol/L is housed, the E.C. 3.5.3.3 of >=30ku/L, the amino antipyrine of the 4-of px >=1ku/L and 2.95mmol/L in second reagent bottle.

Compared with prior art, the advantage of the utility model is: reagent bottle combination is simple, has simplified operation steps, and is highly sensitive, detected result is accurate, the pollution of allogenic material in not receiving, and it is convenient to have accumulating simultaneously, is fit to the advantage of rapid determination.

Description of drawings

Fig. 1 is embodiment 1 structural representation.

Fig. 2 is the exploded view of Fig. 1.

Fig. 3 is the enlarged view of first reagent bottle among Fig. 2.

Fig. 4 is the enlarged view of second reagent bottle among Fig. 2.

Embodiment

Embodiment describes in further detail the utility model below in conjunction with accompanying drawing.

Like Fig. 1, Fig. 2, Fig. 3 and shown in Figure 4, the creatinine assay test kit in the present embodiment comprises a box body 4, can open and close the lid of box body 4 and is positioned over four first reagent bottles 1 and four second reagent bottles 2 in the box body 4 side by side.Lid comprises first lid 31, second lid 32 and the 3rd lid 33.For ease of identification, the outside surface of box body 4 is pasted with barcode and indicates 5.

First reagent bottle 1 comprises first bottle 12 and first bottle cap 11 that is flat-shaped; First bottle, 12 upper surfaces are convexly equipped with and are formed with first threaded port 121 adaptive with first bottle cap 11; Second reagent bottle 2 comprises that second bottle 22 that is column and second bottle cap, 21, the second bottles, 22 upper surfaces are convexly equipped with and is formed with second threaded port 221 adaptive with second bottle cap 21.

Inspection principle: the hydrolysis under the effect of Creatininase of the creatinine in the sample generates creatine; Creatine further generates sarkosine under the catalysis of E.C. 3.5.3.3; Sarkosine oxidation under the effect of sarcosine oxidase produces hydrogen peroxide, and hydrogen peroxide produces mauve quinonimine (TrinderShi reaction) with N-ethyl-N-(2-hydroxyl-3-thiopropyl)-3-monomethylaniline (TOOS), the amino antipyrine of 4-(4-AAP) reaction under catalatic effect.Under the 546nm wavelength, its change in color is directly proportional with the content of creatinine.

Operation steps: sample 5 μ l, add the reagent 225 μ l in first reagent bottle, reading after 3～5 minutes adds the reagent 75 μ l in second reagent bottle, and reading then through automatic biochemistry analyzer, obtains the result once more.

Claims

1. creatinine assay test kit; It is characterized in that this test kit comprises a box body, can open and close the lid of box body and is positioned over intravital four first reagent bottles of box and four second reagent bottles side by side; Described first reagent bottle comprises first bottle and first bottle cap that is flat-shaped; The aforesaid first bottle upper surface is convexly equipped with and is formed with first threaded port adaptive with first bottle cap; Described second reagent bottle comprises second bottle and second bottle cap that is column, and the aforesaid second bottle upper surface is convexly equipped with and is formed with second threaded port adaptive with second bottle cap.

2. test kit according to claim 1 is characterized in that the outside surface of said box body is pasted with the barcode sign.