CN202576429U - Cell culture array chip with functions of microflow diffusion and open intervention - Google Patents
Cell culture array chip with functions of microflow diffusion and open intervention Download PDFInfo
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- CN202576429U CN202576429U CN 201220016043 CN201220016043U CN202576429U CN 202576429 U CN202576429 U CN 202576429U CN 201220016043 CN201220016043 CN 201220016043 CN 201220016043 U CN201220016043 U CN 201220016043U CN 202576429 U CN202576429 U CN 202576429U
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/16—Microfluidic devices; Capillary tubes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/20—Material Coatings
Abstract
The utility model relates to a cell culture array chip with functions of microflow diffusion and open intervention. The cell culture array chip with functions of microflow diffusion and open intervention is applicable to cell culture, stem cell proliferation, stem cell differentiation, drug screening, regenerative medicine and the like and comprises a base and a substrate fixed thereon. The substrate is evenly provided with a plurality of pore-shaped cell culture units. The substrate is provided with a microflow master channel and a microflow diffusion channel which are communicated with each other. The microflow master channel and the microflow diffusion channel are communicated with each cell culture unit and the outside. The cell culture array chip with functions of microflow diffusion and open intervention has the advantages that required culture solution, growth factors and the like are provided by a microflow diffusion network, cell shear force is zero, and gas and humidity required by cell growth are guaranteed; micro-environment multichannel microfluidic concentration gradient of cells is simulated in vivo, and functions of timely, qualitative release and the like of drugs are realized; the cell culture array chip with functions of microflow diffusion and open intervention is applicable to high-flux drug screening research of cell phenotypes; and the cell culture array chip with functions of microflow diffusion and open intervention is compact in structure, low in cost and producible on a large scale.
Description
Technical field
The utility model relates to a kind of miniflow diffusion and the open cell culture array chip of getting involved; Be applicable to cell cultures, stem cells hyperplasia and differentiation, cellular system engineering, drug screening, regenerative medicine, micro-fluidic and nanotechnology etc., belong to biological technical field.
Background technology
In the research of cytobiology, cell cultures is very crucial.Though also can realize the cultivation and the amplification of cell with traditional petridish, the true environment of the mode of this static cultivation and animal body inner cell has very big difference.Fact proved that in animal tissues's structure, the metabolism of cell is dynamic, i.e. its nutrition and other growth factor are to carry through blood vessel, and the mode with the miniflow diffusion is delivered to cell again.Thus, the life of most of cells in vivo keeps and increases and be based on miniflow diffusion.In recent years, many reports about cell cultures under the miniflow condition are arranged.But these work are filling type basically, i.e. the nutritive medium microchannel or the miniflow cavity at cell place of directly flowing through, thus introduce the shearing force that is unfavorable for the cell growth.Therefore, the miniflow of filling type is cultivated also true environment with cells in vivo has big difference very.In addition, in the micro flow chip of sealing, the growing environment of cell will receive very big influence, and culture condition also is difficult to control, also therefore can cause the slow or apoptosis of cell proliferation.But micro flow chip is used for cell cultures has its special advantages.For example, available micro flow chip carries out high-throughout cell analysis etc.
In sum, miniflow cell cultures mode traditional and filling type is incomplete.Though the mode of miniflow regulation and control is fine, there is basic defective in the plan that the cells involved that is developed is up to now cultivated, has caused its application limitation.In fact, the miniflow cell cultures is current still to rest on breadboard conceptual phase, also has very big one section distance from clinical application.
Summary of the invention
The purpose of the utility model is, overcomes the shortcoming of prior art, and a kind of miniflow diffusion and the open cell culture array chip of getting involved are provided.Said cell culture array chip is made up of the open cell culture unit array of a plurality of multiple; Each cell culture unit all with miniflow main channel and miniflow diffusion admittance UNICOM; Making required nutrient solution, growth factor or medicine is to provide through the miniflow proliferation network that links to each other with the external world, is zero thereby make the cell shearing force; Each cell culture unit is suitable for reading open to outside atmosphere, has guaranteed required gas and the humidity environment of cell growth effectively; The multichannel micro-fluidic concentration gradient of analog cell microenvironment in vivo realizes functions such as the timing of medicine, quantitative release; Be specially adapted to high-throughout drug screening research work, can be widely used in basic cell research and clinical diagnosis field based on cell phenotype; Simultaneously, chip structure is compact, and cost is low, and can be mass-produced; The intravital capillary vessel of the similar animal in the main channel of this miniflow proliferation network, relevant cell factor is realized through the miniflow diffusion admittance that links to each other with the main channel; The utility model is emulation, dynamic, open and the research cell miniflow dispersion pattern, and efficient is high, steady quality.
A kind of miniflow diffusion and the open a kind of technical scheme of cell culture array chip that gets involved of the utility model are:
A kind of miniflow diffusion and the open cell culture array chip of getting involved, it comprises a substrate and is fixed on suprabasil substrate; Be distributed with the cell culture unit of several hole shapes on the said substrate; Each cell culture unit one end has open suitable for reading up, and the other end end opening is towards substrate surface; Several cell culture units are formed open cell culture unit array; Cellular miniflow main channel and miniflow diffusion admittance that mutual UNICOM is arranged on substrate and the plane that substrate combines; And each cell culture unit of UNICOM; Be used for cell culture fluid or cytokine or medicine are injected the miniflow main channel at regular time and quantity through single channel or multiplexer channel; And carry out exchange of substance through miniflow diffusion admittance and cell culture unit, and cell is in zero flow rate regime; Each cell culture unit is suitable for reading to communicate with external environment, is used for required gas and the humidity of regulating cell growth.
Further technical scheme is on the technique scheme basis:
Miniflow main channel and miniflow diffusion admittance on diffusion of described miniflow and the open cell culture array chip of getting involved, described substrate and plane that substrate combines are the 3 dimensional coil geometry of chemically modified.
Described miniflow diffusion and the open cell culture array chip of getting involved; Its cell culture unit is surrounded by Polygons or circular miniflow main channel; And by miniflow diffusion admittance UNICOM's cell culture unit and miniflow main channel; Be used to realize multichannel micro-fluidic concentration gradient, make the medicine timing, quantitatively discharge, realize high-throughout drug screening.
Described miniflow diffusion and the open cell culture array chip of getting involved, there are integrated Micropump and little valve in its fluid inlet and outlet place, its miniflow main channel, is used to realize the timing of chemical substance, quantitatively injection.
Described miniflow diffusion and the open cell culture array chip of getting involved; Its its material of substrate that constitutes the cell culture unit array is the bio-compatible macromolecular material; Be selected from polymer poly dimethyl siloxane PDMS; Or the thermoplastic material polymetylmethacrylate, or polycarbonate, or PS PS.
Described miniflow diffusion and the open cell culture array chip of getting involved, its miniflow diffusion admittance height is smaller or equal to 5 microns, and width is smaller or equal to 5 microns; Miniflow main channel height is smaller or equal to 200 microns, and width is smaller or equal to 500 microns; The diameter of cell culture unit is more than or equal to 0.2 millimeter, and smaller or equal to 10 millimeters, height is smaller or equal to 8 millimeters.
Described miniflow diffusion and the open cell culture array chip of getting involved; Its material of described substrate is a biocompatible materials; Be selected from sheet glass; Or the inclined to one side plain film processed of polymer poly dimethyl siloxane PDMS or thermoplastic material polymetylmethacrylate or polycarbonate or PS PS, or flat vessel.
Described miniflow diffusion and the open cell culture array chip of getting involved are implanted the components and parts that electricity is arranged in the described cell culture unit array, and/or the components and parts of magnetic, and/or the components and parts of light, or the components and parts combined chip.
A kind of miniflow diffusion and the another kind of technical scheme of the open cell culture array chip of getting involved of the utility model are: it comprises a substrate and is fixed on suprabasil substrate; Be distributed with the cell culture unit of several hole shapes on the said substrate; Each cell culture unit one end has open suitable for reading up, and the other end end opening is towards substrate surface; Several cell culture units are formed open cell culture unit array; Cellular miniflow main channel and miniflow diffusion admittance that mutual UNICOM is arranged on substrate and the plane that substrate combines, and each cell culture unit of UNICOM; Each cell culture unit is suitable for reading to communicate with external environment, is used for required gas and the humidity of regulating cell growth.
The obvious technical effects of the utility model: the cell culture fluid in the miniflow main channel, growth factor or medicine are regulated and control through real-time carrying out accurately.In whole culturing process, the metabolism of cell is spread through miniflow and is accomplished, and therefore, acting on the cell shearing force is zero; Suitable for reading and extraneous the communicating of each cell culture unit, thus required gas and the humidity environment of cell growth effectively guaranteed; For traditional static cell culture system; Can be used for analog cell microenvironment in vivo based on miniflow diffusion and the open cell culture array of getting involved; Thereby be relative research and application; Particularly stem cells hyperplasia and differentiation, drug screening, cellular system engineering, regenerative medicine etc. provide new ways and means; With respect to existing miniflow cell culture system, avoid the restriction of the suffered shearing force of cell based on miniflow diffusion and the open cell culture array of getting involved, and be convenient to regulation and control and analysis, more can satisfy the needs of Bioexperiment; According to the needs of concrete experiment, can change the design of miniflow main channel, multichannel micro-fluidic concentration gradient realizes functions such as the timing of medicine, quantitative release; The utility model is specially adapted to the high-throughout drug screening research work based on cell phenotype, can be widely used in basic cell research and clinical diagnosis field; Also have, the making of the utility model chip is simple, and cost is low, and can produce in enormous quantities.
Description of drawings
Fig. 1 is the A-A view of Fig. 2; Be to look up from bottom to top that the cell culture unit 1 of substrate 5 before being bonded on substrate 2 planes concerns with the position between miniflow diffusion admittance 3 and the miniflow main channel 4 and the view of UNICOM's approach, this has also showed the position of fluid inlet and outlet 7;
Fig. 2 looks (side direction) synoptic diagram for the master of diffusion of the utility model miniflow and the open cell culture array chip structure of getting involved; Showed the relation between the substrate 2 of the substrate 5 on upper strata and lower floor; Simultaneously, local breaking part has been showed the link relation of cell culture unit 1 and miniflow diffusion admittance 3, has also showed the position of fluid inlet and outlet 7: when the view person towards Fig. 2; The left side is during as fluid inlet; Then the right side is as fluid outlet, and perhaps right conversely is import, and then a left side is outlet;
Fig. 3 is the schematic top plan view of Fig. 2; Identical position relation and the array status of cell culture unit 1 suitable for reading 1.1 between the substrate 2 of the substrate 5 on upper strata and lower floor have been showed; Simultaneously, local breaking part has been showed position relation and the UNICOM's approach between cell culture unit 1 and miniflow diffusion admittance 3 and miniflow main channel 4 and the fluid inlet and outlet 7; Said local the disconnection is to break off along miniflow diffusion admittance 3 medullary rays are local;
Fig. 4 is the M portion partial enlarged drawing of Fig. 1, is to show the cell culture array of several cell culture units 1 formations and the relative position relation synoptic diagram of miniflow diffusion admittance 3 and miniflow main channel 4;
Fig. 5 overlooks enlarged view for the material object of 1 cell culture unit 1 of the utility model;
Fig. 6 is the B-B cross-sectional schematic of Fig. 5, has showed upper-lower position relation and 1 cell culture unit 1 substrate 5 on and the position of miniflow diffusion admittance 3 and miniflow main channel 4 relative positions and link relation and suitable for reading 1.1 of substrate 5 with substrate 2;
Fig. 7 is placed on the picture in the dish for the cell culture array chip material object of diffusion of the utility model miniflow and open intervention.
The name of each Reference numeral is called among the figure: the 1-cell culture unit; 1.1-it is suitable for reading; The 2-substrate; 3-miniflow diffusion admittance; 4-miniflow main channel; The 5-substrate; 6-cell culture fluid and/or cytokine and/or medicine; The 7-fluid inlet and outlet.
Embodiment
In conjunction with accompanying drawing the utility model is described further as follows:
Embodiment 1:Shown in Fig. 1-7, be a kind of miniflow diffusion and open first more excellent embodiment of cell culture array chip that gets involved of the utility model.Said miniflow diffusion and the open cell culture array chip of getting involved, it comprises a substrate 2 and is fixed on the substrate 5 in the substrate 2; Be distributed with the cell culture unit 1 of several hole shapes on the said substrate 5; Each cell culture unit 1 one end has up open suitable for reading 1.1, and the other end end opening is towards substrate 2 surfaces; Several cell culture units 1 are formed open cell culture unit array; Cellular miniflow main channel 4 and miniflow diffusion admittance 3 that mutual UNICOM is arranged on substrate 5 and the plane that substrate 2 combines; And each cell culture unit 1 of UNICOM; Be used for cell culture fluid or cytokine or medicine are injected miniflow main channel 4 at regular time and quantity through single channel or multiplexer channel; And carry out exchange of substance through miniflow diffusion admittance 3 and cell culture unit 1, and cell is in zero flow rate regime; Each cell culture unit 1 suitable for reading 1.1 communicates with external environment, is used for required gas and the humidity of regulating cell growth.Miniflow main channel 4 on described substrate 5 and the plane that substrate 2 combines is the 3 dimensional coil geometry of chemically modified with miniflow diffusion admittance 3.Described cell culture unit 1 is surrounded by Polygons or circular miniflow main channel 4; Present embodiment surrounds the miniflow main channel 4 of cell culture unit 1 for waiting hexagon; Also can select octagon or circle,, be used to realize multichannel micro-fluidic concentration gradient by miniflow diffusion admittance 3 UNICOM's cell culture units 1 and miniflow main channel 4; Make the medicine timing, quantitatively discharge, realize high-throughout drug screening.There are integrated Micropump and little valve in 4 its fluid inlet and outlet 7 places, described miniflow main channel, are used to realize the timing of chemical substance, quantitatively injection.Substrate 5 its materials of described formation cell culture unit array are the bio-compatible macromolecular material; Be selected from polymer poly dimethyl siloxane PDMS; Or thermoplastic material polymetylmethacrylate; Or polycarbonate, or PS PS, present embodiment is elected polymer poly dimethyl siloxane PDMS as.Described miniflow diffusion admittance 3 height are smaller or equal to 5 microns, and width is smaller or equal to 5 microns; Miniflow main channel 4 height are smaller or equal to 200 microns, and width is smaller or equal to 500 microns; The diameter of cell culture unit 1 is more than or equal to 0.2 millimeter, and smaller or equal to 10 millimeters, height is smaller or equal to 8 millimeters, and present embodiment is elected as: miniflow diffusion admittance 3 highly is 3 microns, and width is 4 microns; Miniflow main channel 4 highly is 100 microns, and width is 200 microns; The diameter of cell culture unit 1 equals 2 millimeters, 5 millimeters of the height of cell culture unit 1.Described substrate 2 its materials are biocompatible materials; Be selected from sheet glass; Or the inclined to one side plain film processed of polymer poly dimethyl siloxane PDMS or thermoplastic material polymetylmethacrylate or polycarbonate or PS PS, or flat vessel, present embodiment is elected sheet glass as.Implant the components and parts that electricity is arranged in the described cell culture unit array, and/or the components and parts of magnetic, and/or the components and parts of light, or the components and parts combined chip.Present embodiment is elected little electric control element and microelectrode, Micropump and little valve as, can also select one or more combinations of little electric control element, microelectrode, Micropump, little valve, low-light control elements, little magnetic control element.
Embodiment 2:Different with the foregoing description 1 is: substrate 5 its materials of described formation cell culture unit array are the thermoplastic material polymetylmethacrylate.Described miniflow diffusion admittance 3 highly equals 5 microns, and width is 5 microns; Miniflow main channel 4 highly equals 200 microns, and width equals 480 microns; The diameter of cell culture unit 1 equals 10 millimeters, and the height of cell culture unit 1 equals 8 millimeters; Described substrate 2 its materials are the thermoplastic material polymetylmethacrylate, the inclined to one side plain film that also can select polycarbonate or PS PS to process.Implanting in the described cell culture unit array has the electric components and parts and the components and parts of light, is microelectrode, Micropump and little valve, little electric control element, low-light control elements.
Embodiment 3:Be a kind of miniflow diffusion and open second more excellent basic embodiment of cell culture array chip that gets involved of the utility model.Different with embodiment 1 is: the cellular miniflow main channel 4 and miniflow diffusion admittance 3 of mutual UNICOM are arranged on substrate 2 and the plane that substrate 5 combines, and each cell culture unit 1 of UNICOM; Miniflow main channel 4 on described substrate 2 and the plane that substrate 5 combines is the 3 dimensional coil geometry of chemically modified with miniflow diffusion admittance 3.Just: miniflow main channel 4 and miniflow diffusion admittance 3 are produced in the substrate 2 on the plane, have only cell culture unit 1 on the substrate 5, and the lower plane of substrate 5 have only the end opening of cell culture unit 1.Present embodiment at the bottom of the bonding radical 2 when the substrate 5, the location of cell culture unit 1 is extremely important, once dislocation, cell culture unit 1 and miniflow main channel 4 and miniflow diffusion admittance 3 just can not fine UNICOMs, can influence result of use.
The making method miniflow diffusion of the utility model and the making method of the open cell culture array chip of getting involved, basic step comprises:
A, making substrate 5:
1) makes formpiston: draw earlier and making optics template, comprise miniflow main channel 4 and miniflow diffusion admittance 3 and cell culture unit 1, and distinguish with different colours; Make the formpiston that comprises miniflow main channel 4 and miniflow diffusion admittance 3 with the method for optical exposure and ion etching then;
2) molded and shaped substrate: through the method positive replica of soft lithographic and hot padding, process the PDMS that has miniflow main channel 4 and miniflow diffusion admittance 3 and have cell culture unit 1 relative position mark, or PMMA, or PC, or the PS substrate;
3) processing cell culture unit 1 and fluid inlet and outlet 7: get through on-chip each cell culture unit 1 through microfabrication lathe location and import and export 7 with corresponding fluids;
B, bonding substrate 5 and substrate 2: the substrate 5 that will have the miniflow main channel 4 and the cell culture unit array of miniflow diffusion admittance 3 and cell culture unit 1 is bonded on substrate 2 surfaces.
The utility model claim protection domain is not limited to the foregoing description.
Claims (8)
1. a miniflow diffusion and the open cell culture array chip of getting involved is characterized in that it comprises a substrate (2) and is fixed on the substrate (5) in the substrate (2); Be distributed with the cell culture unit (1) of several hole shapes on the said substrate (5); Each cell culture unit (1) one end has open (1.1) suitable for reading up, and the other end end opening is towards substrate (2) surface; Several cell culture units (1) are formed open cell culture unit array; Cellular miniflow main channel (4) and miniflow diffusion admittance (3) that mutual UNICOM is arranged on substrate (5) and the plane that substrate (2) combines; And each cell culture unit of UNICOM (1); Be used for cell culture fluid or cytokine or medicine are injected miniflow main channel (4) at regular time and quantity through single channel or multiplexer channel; And carry out exchange of substance through miniflow diffusion admittance (3) and cell culture unit (1), and cell is in zero flow rate regime; Each cell culture unit (1) (1.1) suitable for reading communicates with external environment, is used for required gas and the humidity of regulating cell growth.
2. miniflow diffusion as claimed in claim 1 and the open cell culture array chip of getting involved; It is characterized in that; Cell culture unit (1) is surrounded by Polygons or circular miniflow main channel (4), and by miniflow diffusion admittance (3) UNICOM's cell culture unit (1) and miniflow main channel (4), is used to realize multichannel micro-fluidic concentration gradient; Make the medicine timing, quantitatively discharge, realize high-throughout drug screening.
3. miniflow diffusion as claimed in claim 1 and the open cell culture array chip of getting involved is characterized in that its fluid inlet and outlet of miniflow main channel (4) (7) has been located integrated Micropump and little valve, are used to realize the timing of chemical substance, quantitatively injection.
4. miniflow diffusion as claimed in claim 1 and the open cell culture array chip of getting involved; It is characterized in that; Its material of substrate (5) that constitutes the cell culture unit array is the bio-compatible macromolecular material, is selected from polymer poly dimethyl siloxane PDMS, or the thermoplastic material polymetylmethacrylate; Or polycarbonate, or PS PS.
5. miniflow diffusion as claimed in claim 1 and the open cell culture array chip of getting involved is characterized in that miniflow diffusion admittance (3) height is smaller or equal to 5 microns, and width is smaller or equal to 5 microns; Miniflow main channel (4) height is smaller or equal to 200 microns, and width is smaller or equal to 500 microns; The diameter of cell culture unit (1) is more than or equal to 0.2 millimeter, and smaller or equal to 10 millimeters, height is smaller or equal to 8 millimeters.
6. miniflow diffusion as claimed in claim 1 and the open cell culture array chip of getting involved; It is characterized in that; Its material of described substrate (2) is a biocompatible materials; Be selected from sheet glass, or the inclined to one side plain film processed of polymer poly dimethyl siloxane PDMS or thermoplastic material polymetylmethacrylate or polycarbonate or PS PS, or flat vessel.
7. miniflow diffusion as claimed in claim 1 and the open cell culture array chip of getting involved is characterized in that, implant the components and parts that electricity is arranged in the described cell culture unit array, and/or the components and parts of magnetic, and/or the components and parts of light, or the components and parts combined chip.
8. a miniflow diffusion and the open cell culture array chip of getting involved is characterized in that it comprises a substrate (2) and is fixed on the substrate (5) in the substrate (2); Be distributed with the cell culture unit (1) of several hole shapes on the said substrate (5); Each cell culture unit (1) one end has open (1.1) suitable for reading up, and the other end end opening is towards substrate (2) surface; Several cell culture units (1) are formed open cell culture unit array; Cellular miniflow main channel (4) and miniflow diffusion admittance (3) that mutual UNICOM is arranged on substrate (2) and the plane that substrate (5) combines, and each cell culture unit of UNICOM (1); Each cell culture unit (1) (1.1) suitable for reading communicates with external environment, is used for required gas and the humidity of regulating cell growth.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103103121A (en) * | 2013-01-17 | 2013-05-15 | 中国科学院深圳先进技术研究院 | Cell-culture microfluidic chip |
| CN103667054A (en) * | 2013-09-18 | 2014-03-26 | 中国航天员科研训练中心 | Integrated microfluidic cell culture chip and preparation method thereof |
| CN103992948A (en) * | 2014-05-30 | 2014-08-20 | 南京农业大学 | Micronanofluidic device for researching cell migration |
| CN105080627A (en) * | 2015-08-25 | 2015-11-25 | 辽宁中医药大学 | Integrated microfluidic chip for screening medicine and method for applying integrated microfluidic chip |
| CN105349420A (en) * | 2015-11-27 | 2016-02-24 | 大连理工大学 | Bionic microfluidic cell culture chip |
| CN110586210A (en) * | 2019-09-11 | 2019-12-20 | 河海大学常州校区 | Concentration gradient generation device for gel 3D cell culture, preparation and application method |
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2012
- 2012-01-13 CN CN 201220016043 patent/CN202576429U/en not_active Expired - Fee Related
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103103121A (en) * | 2013-01-17 | 2013-05-15 | 中国科学院深圳先进技术研究院 | Cell-culture microfluidic chip |
| CN103103121B (en) * | 2013-01-17 | 2014-10-08 | 中国科学院深圳先进技术研究院 | Cell-culture microfluidic chip |
| CN103667054A (en) * | 2013-09-18 | 2014-03-26 | 中国航天员科研训练中心 | Integrated microfluidic cell culture chip and preparation method thereof |
| CN103992948A (en) * | 2014-05-30 | 2014-08-20 | 南京农业大学 | Micronanofluidic device for researching cell migration |
| CN105080627A (en) * | 2015-08-25 | 2015-11-25 | 辽宁中医药大学 | Integrated microfluidic chip for screening medicine and method for applying integrated microfluidic chip |
| CN105349420A (en) * | 2015-11-27 | 2016-02-24 | 大连理工大学 | Bionic microfluidic cell culture chip |
| CN110586210A (en) * | 2019-09-11 | 2019-12-20 | 河海大学常州校区 | Concentration gradient generation device for gel 3D cell culture, preparation and application method |
| CN110586210B (en) * | 2019-09-11 | 2021-10-08 | 河海大学常州校区 | Concentration gradient generation device for gel 3D cell culture, preparation and application method |
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