CN202543217U - Kinetic parameter measurement device of autotrophic bacteria - Google Patents

Kinetic parameter measurement device of autotrophic bacteria Download PDF

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Publication number
CN202543217U
CN202543217U CN 201220165744 CN201220165744U CN202543217U CN 202543217 U CN202543217 U CN 202543217U CN 201220165744 CN201220165744 CN 201220165744 CN 201220165744 U CN201220165744 U CN 201220165744U CN 202543217 U CN202543217 U CN 202543217U
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reaction chamber
meter
dissolved oxygen
autotrophic bacteria
kinetic parameter
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CN 201220165744
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李志华
王之玺
姬晓琴
王晓昌
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Xian University of Architecture and Technology
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Xian University of Architecture and Technology
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/32—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of substances in solution
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/44—Means for regulation, monitoring, measurement or control, e.g. flow regulation of volume or liquid level

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  • Activated Sludge Processes (AREA)

Abstract

The utility model discloses a kinetic parameter measurement device of autotrophic bacteria. The device comprises a reaction chamber and a sludge tank, wherein the top wall of the reaction chamber is communicated with the sludge tank via an electrically operated valve, a water bath circulation system is arranged at the peripheral wall of the reaction tank, a stirring device is arranged along the top wall of the reaction chamber and extends into the bottom of a tank body of the reaction chamber, a pH meter, a dissolved oxygen instrument and a liquid meter are respectively arranged in the reaction chamber, an aeration sand core is arranged at the bottom of the reaction chamber and is communicated with an aeration pump, the electrically operated valve, the stirring device, the pH meter, the dissolved oxygen instrument and the liquid meter are respectively connected with a programmable logic controller (PLC) control box, and the PLC control box is connected with the aeration pump and a computer respectively. The device is used for correcting concentration of saturated dissolved oxygen, recording data in real time, drawing a curve line, fitting an error and evaluating fitting quality. Intelligence of control and numerical value analysis is realized, and the device is simple to operate.

Description

A kind of autotrophic bacteria kinetic parameter measuring apparatus
Technical field
The utility model belongs to sewage treatment area, relates to a kind of autotrophic bacteria kinetic parameter measuring apparatus.
Background technology
Pollutents such as using microbe degradation of organic substances, ammonia nitrogen in the WWT, in the process of contaminant degradation, microorganisms grow.Along with the research to microorganism treating sewage is goed deep into, the microbial kinetics model is introduced in the water treatment, has formed one and has overlapped the activated sludge kinetics model that has comprised processes such as biological removal organism, ammonia nitrogen, phosphorus and mikrobe self-dissolving.Activated sludge model has become the design of present sewage work and has operated indispensable important content in service.But the kinetic parameter for the mikrobe in the different sewage factory in practice quantitatively is the accurately prerequisite of utilization of model.And,, make the autotrophic bacteria kinetic parameter lose the meaning of timely control sewage treatment process operational conditions because poor growth is calculated the usually consuming time very long of its kinetic parameter with existing method for autotrophic microorganism.And in some testing method, need calculate the autotrophic bacteria kinetic parameter with it with ammonia nitrogen or the direct measuring parameter of nitre nitrogen concentration conduct, such method needs a large amount of test water quality parameters, wastes time and energy.
The utility model content
The purpose of the utility model is to provide a kind of autotrophic bacteria kinetic parameter measuring apparatus; Realize the control software for calculation through this device; Overcome the autotrophic bacteria kinetic parameter and test the problem of length consuming time, inconvenient operation, realize at short notice, just can obtain correlation parameter through easy operation.
To achieve these goals, the technical scheme of the utility model employing is:
A kind of autotrophic bacteria kinetic parameter measuring apparatus comprises reaction chamber, reaches the sludge tank that is communicated with through motorized valve with the reaction chamber roof, and said reaction chamber tank body perisporium is provided with the water-bath recycle system; Be provided with the whisking appliance that extends to the reaction chamber tank base along said reaction chamber roof, be respectively arranged with pH meter, dissolved oxygen meter and liquidometer in the reaction chamber; Said reaction chamber bottom is provided with the aeration core that is communicated with aeration pump; Said motorized valve, whisking appliance, pH meter, dissolved oxygen meter and liquidometer link to each other with the PLC controlling box respectively, and the PLC controlling box connects aeration pump and computingmachine respectively.
As the improvement of the utility model device, further:
The said water-bath recycle system comprises the water-bath chamber that is arranged on the reaction chamber perisporium, and the water-bath chamber is communicated with formation water-bath loop through lift pump with clean water basin, and said clean water basin link to each other with refrigeration mechanism with the system heating arrangements.
Said heating arrangements and refrigeration mechanism comprise semiconductor chilling plate and electronic temperature controller.
Be respectively arranged with transmitter on said pH meter and the dissolved oxygen meter.
Can be implemented in the test process through this device, realize the robotization of aeration and sample introduction through the water outlet motorized valve of the aeration agitation system in the intelligence control system gear, sludge tank.And system can judge that aeration begins or stops according to the measured dissolved oxygen concentration of dissolved oxygen electrode.After accomplishing test, can directly calculate relevant parameter through WinForm_DO_Meter software, obtain the result.
The utility model compared with prior art has the following advantages:
1) weak point consuming time
Because autotrophic bacteria stretches slowly, need the time long to the mensuration of its kinetic parameter, the utility model has significantly shortened the test duration through a kind of new mathematical computations mode; Autotrophic bacteria and heterotrophic bacterium growth compared are slow, usually its kinetic parameter are carried out needing long continuous monitoring when quantitative, and general consuming time is about 15 days, the shortest also at 7 days.And after adopting present method, a test duration just can accomplish about 6 hours.
2) data analysis is convenient and reliable
Adopt the fluorescent method dissolved oxygen electrode to gather the dissolved oxygen data, to dissolved oxygen change sensitive and response fast, the dissolved oxygen data of collection are precisely intensive, weak point can be made as per second and write down a secondary data.With WinForm_DO_Meter the change curve DS of dissolved oxygen is analyzed except can obtaining serial kinetic parameter, can also be calculated oxygen mass transfer coefficients, saturated dissolved oxygen, and can add other calculation control module as required.
3) realize control automatically
Cooperated robot control system(RCS), the WinForm_DO_Meter software of design voluntarily to realize the intellectuality of control, numerical analysis on the test set, simple to operate.
The utility model can national main cities to local saturated dissolved oxygen concentration proofread and correct, dissolved oxygen concentration data logging and curve plotting in real time.And can carry out local aerobic speed, the aerobic speed on the specific data interval, dynamic OUR, the oxygen mass transfer coefficients K at each data point place LA, saturated dissolved oxygen C ∞, automatically actual measurement dissolved oxygen data are carried out match and are provided error of fitting, it is good and bad to estimate match.
Description of drawings
Fig. 1 is the utility model measuring apparatus synoptic diagram.
Fig. 2 is a WinForm_DO_Meter user interface of software synoptic diagram.
Among the figure: 1 is sludge tank, and 2 is motorized valve, and 3 is whisking appliance, and 4 is the pH transmitter, and 5 is the dissolved oxygen meter transmitter; 6 is liquidometer, and 7 is reaction chamber, and 8 are the water-bath chamber, and 9 is the aeration core, and 10 is clean water basin; 11 is aeration pump, and 12 is lift pump, and 13 is the PLC controlling box, and 14 is computingmachine.
Embodiment
Through accompanying drawing and embodiment the utility model is done further explanation below.
As shown in Figure 1, for realizing the device of the utility model autotrophic bacteria kinetic parameter measuring method, comprise reaction chamber 7, reach the sludge tank 1 that is communicated with through motorized valve 2 with reaction chamber 7 roofs, wherein: reaction chamber 7 tank body perisporiums are provided with the water-bath recycle system; Be provided with the whisking appliance 3 that extends to reaction chamber 7 tank base along reaction chamber 7 roofs, be respectively arranged with pH meter 4, dissolved oxygen meter 5 and liquidometer 6 in the reaction chamber 7; Reaction chamber 7 bottoms are provided with the aeration core 9 that is communicated with aeration pump 11; Motorized valve 2, whisking appliance 3, pH meter 4, dissolved oxygen meter 5 and liquidometer 6 link to each other with PLC controlling box 13 respectively, and PLC controlling box 13 connects aeration pump 11 and computingmachine 14 respectively.The water-bath recycle system comprises the water-bath chamber 8 that is arranged on reaction chamber 7 perisporiums; Water-bath chamber 8 is communicated with formation water-bath loop through lift pump 12 with clean water basin 10; Clean water basin 10 link to each other with refrigeration mechanism with the system heating arrangements, and heating arrangements and refrigeration mechanism comprise semiconductor chilling plate and electronic temperature controller.Be respectively arranged with transmitter on pH meter 4 and the dissolved oxygen meter 5.
Microbe-derived as autotrophic bacteria with the municipal wastewater treatment plant active sludge that is in the endogenous respiration state through the utility model below, concentration is that the ammonia nitrogen solution of the 5～25mg/L instance during as the autotrophic bacteria matrix solution is explained the measuring process that realizes the autotrophic bacteria kinetic parameter.
Testing method comprises three key steps: aerobic rate test stage of endogenous respiration, oxygen mass transfer coefficient test phase and nitration reaction external source respiratory rate test phase; Three steps all are the variations through dissolved oxygen concentration in the real-time monitoring respirometer, obtain the aerobic speed of endogenous respiration, oxygen mass transfer coefficient, external source respectively and breathe aerobic speed.Wherein nitration reaction external source respiratory rate test phase comprises and adds quantitative ammonia nitrogen several times, up to the repeating step of ammonia nitrogen completely consumed.Each ammonia nitrogen consumption finishes, and can calculate corresponding biological increment by the ammonia nitrogen amount that is consumed, and after adding ammonia nitrogen again, and this part biological increment can make the maximum rate of nitrification that adds once more behind the ammonia nitrogen become big.Through adding the variation and the biological increment of the maximum rate of nitrification behind the ammonia nitrogen for twice, can obtain high specific increment speed μ A, maxAnd the autotrophic bacteria living weight X in the active sludge B, A
This method is carried out according to the following steps:
1) the water-bath recycle system of unlatching reaction chamber 7 sidewalls, water-bath is reached needs temperature; In air, dissolved oxygen meter 5 is proofreaied and correct the log-on data writing function;
2) in sludge tank 1, add mud, open motorized valve 2, mud mixed liquid can weight flow into reaction chamber 7 certainly in the sludge tank, changes test endogenous respiration speed, oxygen mass transfer coefficients and saturated dissolved oxygen concentration through dissolved oxygens in the dissolved oxygen meter monitoring reaction chambers 75;
3) feed quantitative ammonia nitrogen from aeration core 9 aeration pumps 11, connect PLC controlling box 13 match monitoring data, and monitor value is inputed to computingmachine 14 monitoring dissolved oxygens variations through dissolved oxygen meter 5; Can in PLC controlling box 13WinForm_DO_Mete r, aeration time be set, or stop aeration pump according to automatic unlatching of dissolved oxygen concentration;
4) ammonia nitrogen concentration that adds is calculated the biological increment of autotrophic bacteria through the stoichiometric relation in the activated sludge model matrix, obtain article one external source and breathe the dissolved oxygen change curve;
Owing to autotrophic bacteria oxidation ammonia nitrogen oxygen consumption (breathing of autotrophic bacteria external source), dissolved oxygen concentration descends in the test process, and along with ammonia nitrogen is consumed, the autotrophic bacteria external source is breathed and diminished, and dissolved oxygen concentration rises.Treat the concentration value before dissolved oxygen is raised to the adding ammonia nitrogen, this is that article one external source is breathed the dissolved oxygen change curve.Can calculate the biological increment of autotrophic bacteria through the stoichiometric relation in the activated sludge model matrix this moment by the ammonia nitrogen concentration that begins to add;
5) add ammonia nitrogen amount with the step 3) moderate once more, repeating step 3)-4) test, obtain the second external source and breathe the dissolved oxygen change curve;
6) obtain two maximum rate of nitrification r of maximum rate of nitrification by two curve calculation Max, the semi-saturation COEFFICIENT K NH
7) the relatively variation of two maximum rates of nitrification can obtain the autotrophic bacteria living weight X in the former active sludge B, AAnd high specific increment speed μ A, max
Can calculate two maximum rate of nitrification r of maximum rate of nitrification by two curves Max, the semi-saturation COEFFICIENT K NH, second rate of nitrification can be bigger than first rate of nitrification, this is owing to the biological increment of autotrophic bacteria causes, promptly by the first time, the ammonia nitrogen consumption was calculated to.Relatively the variation of two maximum rates of nitrification can obtain the autotrophic bacteria living weight X in the former active sludge B, AAnd high specific increment speed μ A, max
Can through saturated dissolved oxygen concentration is proofreaied and correct, dissolved oxygen concentration data logging and curve plotting in real time.And can carry out local aerobic speed, the aerobic speed on the specific data interval, dynamic OUR, the oxygen mass transfer coefficients K at each data point place LA, saturated dissolved oxygen C ∞, automatically actual measurement dissolved oxygen data are carried out match and are provided error of fitting, it is good and bad to estimate match.And software uses modular design can insert modules such as activated sludge kinetics calculating as required.Accompanying drawing 2 is seen at WinForm_DO_Meter software operation interface.
The above; Be merely the preferable embodiment of the utility model; But the protection domain of the utility model is not limited thereto; Any technician who is familiar with the present technique field is in the technical scope that the utility model discloses, and the variation that can expect easily or replacement all should be encompassed within the protection domain of the utility model.

Claims (4)

1. an autotrophic bacteria kinetic parameter measuring apparatus comprises reaction chamber (7), and with reaction chamber (7) roof through the sludge tank (1) that motorized valve (2) is communicated with, it is characterized in that: said reaction chamber (7) tank body perisporium is provided with the water-bath recycle system; Be provided with the whisking appliance (3) that extends to reaction chamber (7) tank base along said reaction chamber (7) roof, be respectively arranged with pH meter (4), dissolved oxygen meter (5) and liquidometer (6) in the reaction chamber (7); Said reaction chamber (7) bottom is provided with the aeration core (9) that is communicated with aeration pump (11); Said motorized valve (2), whisking appliance (3), pH meter (4), dissolved oxygen meter (5) and liquidometer (6) link to each other with PLC controlling box (13) respectively, and PLC controlling box (13) connects aeration pump (11) and computingmachine (14) respectively.
2. a kind of autotrophic bacteria kinetic parameter measuring apparatus according to claim 1; It is characterized in that: the said water-bath recycle system comprises the water-bath chamber (8) that is arranged on reaction chamber (7) perisporium; Water-bath chamber (8) is communicated with formation water-bath loop through lift pump (12) with clean water basin (10), said clean water basin (10) link to each other with refrigeration mechanism with the system heating arrangements.
3. a kind of autotrophic bacteria kinetic parameter measuring apparatus according to claim 2, it is characterized in that: said heating arrangements and refrigeration mechanism comprise semiconductor chilling plate and electronic temperature controller.
4. a kind of autotrophic bacteria kinetic parameter measuring apparatus according to claim 1 is characterized in that: be respectively arranged with transmitter on said pH meter (4) and the dissolved oxygen meter (5).
CN 201220165744 2012-04-18 2012-04-18 Kinetic parameter measurement device of autotrophic bacteria Expired - Fee Related CN202543217U (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102643742A (en) * 2012-04-18 2012-08-22 西安建筑科技大学 Autotrophic bacteria kinetic parameter measurement device and method

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102643742A (en) * 2012-04-18 2012-08-22 西安建筑科技大学 Autotrophic bacteria kinetic parameter measurement device and method
CN102643742B (en) * 2012-04-18 2013-06-05 西安建筑科技大学 Autotrophic bacteria kinetic parameter measurement device and method

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