CN202499839U - Dual-mode optical coding probe - Google Patents
Dual-mode optical coding probe Download PDFInfo
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- CN202499839U CN202499839U CN2012200115976U CN201220011597U CN202499839U CN 202499839 U CN202499839 U CN 202499839U CN 2012200115976 U CN2012200115976 U CN 2012200115976U CN 201220011597 U CN201220011597 U CN 201220011597U CN 202499839 U CN202499839 U CN 202499839U
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Abstract
The utility model discloses a dual-mode optical coding probe. The probe adopts a three-layer core-shell structure, wherein a first-layer core consists of a gold nanorod, a second-layer shell is made from silicon dioxide, a third-layer shell consists of cadmium telluride quantum dots, the second-layer shell is wrapped at the outer side of the first-layer core, the third-layer shell is pasted at the outer side of the second-layer shell, and Raman molecules are pasted on the external surface of the first-layer core and are wrapped by the second-layer shell. The dual-mode optical coding probe has the capability of fluorescence and SERS (Surface Enhanced Raman Scattering) joint coding, so that the optical coding capability is enhanced.
Description
Technical field
The utility model relates to field of nanometer material technology, specifically, relates to a kind of double mode optical encoding probe, and this double mode optical encoding probe possesses the function of fluorescence and surface enhanced Raman scattering combined coding.
Background technology
Along with the continuous improvement of technology of preparing and characterization method, with its unique optical property and undersized characteristics, become the focus that domestic and international investigators pay close attention to gradually based on the optical probe of nano material.The different optical probe of these functions has great application prospect in bio-sensing, bio-imaging and biological detection.
The fluorescence detection technology is quick and simple, is a kind of biological detection means commonly used.There is the inherent shortcoming in traditional organic dye molecule (fluorophore), is prone to bleached like emission spectrum wide ranges, fluorescence etc.The semiconductor-quantum-point material that occurs in recent years has advantages such as spectrum is narrow, light stability is high, the characteristics of luminescence is adjustable, becomes a kind of novel biological fluorescent labelling thing.The technological spectral line of surface enhanced Raman scattering (English is " Surface Enhanced Raman Scattering ", in the literary composition be called for short " SERS ") is narrow, be difficult for photobleaching and harmless to biological tissue.The huge Raman enhancement that surface enhanced Raman scattering has makes the detection based on surface enhanced Raman scattering can reach very high sensitivity, even can realize the detection of single molecules level.
The optical probe of coding can be used for realizing that various biomolecules detects simultaneously.Usually optical encoding is based on fluorescent signal or SERS signal.Be encoded to example with quantum dot fluorescence, can generate about 10 kinds of sign indicating numbers (10 kinds of colors) at visible region (380-780nm) in theory.But because the influence of spectra overlapping and resonance energy trans effect, actually can only prepare 3 kinds of distinguishable sign indicating numbers of left and right sides spectrum.And raman labels similar molecular commonly used, it is overlapping significantly to make that their SERS spectrum also has, and available spectral range is limited.The feasible independent distinguishable sign indicating number of the spectrum number that generates based on the coded system of fluorescent signal or SERS signal of these unfavorable factors far is inferior to theoretical expectation.
Summary of the invention
Technical problem:The utility model technical problem to be solved is: a kind of double mode optical encoding probe is provided; This double mode optical encoding probe sets fluorescence and two kinds of optical signallings of SERS are in one; Make this double mode optical encoding probe possess the ability of fluorescence and SERS combined coding, strengthened the optical encoding ability.
Technical scheme:For solving the problems of the technologies described above the double mode optical encoding probe of the utility model, this probe employing three-layer nuclear shell structure; The first layer nuclear is gold nanorods, and second layer shell is a silicon-dioxide, and the 3rd layer of shell is cadmium telluride quantum dot; Second layer shell is wrapped in the outside of the first layer nuclear; The 3rd layer of shell pastes in the outside of second layer shell, and the outside surface of the first layer nuclear is covered with Raman molecular, and Raman molecular is wrapped up by second layer shell.
Beneficial effect:Compared with prior art, the utlity model has following advantage:
1. integrate two kinds of optical signallings of fluorescence and SERS, make this double mode optical encoding probe possess the ability of fluorescence and SERS combined coding, strengthened the optical encoding ability.In the prior art, the optical encoding probe only has a kind of ability of coding usually, based on fluorescent signal or SERS signal.The optical encoding probe that the present technique scheme provides adopts three-layer nuclear shell structure; The first layer nuclear is gold nanorods, and second layer shell is a silicon-dioxide, and the 3rd layer of shell is cadmium telluride quantum dot; And the outside surface of the first layer nuclear is covered with Raman molecular, and Raman molecular is wrapped up by second layer shell.This optical encoding probe is under excitation light irradiation; Utilize fluorescence spectrum scope and SERS spectral range simultaneously, can produce fluorescence and SERS signal, possess fluorescence and SERS combined coding ability; The actual sign indicating number number that can generate will increase greatly, possess excellent optical encoding ability.The double mode optical encoding probe of this structure has N the cadmium telluride quantum dot that diameter is different; M different types of Raman molecular 2; The number H=of this optical encoding probe codified
has so enlarged the number of codified.
2. strong, the good stability of the optical encoding probe signals of this structure.Do the SERS substrate with traditional spherical gold grain and compare, the present technique scheme adopts gold nanorods to do the SERS substrate, can obtain stronger SERS signal.Compare as fluorescent material with traditional organic fluorescent dye, the present technique scheme adopts cadmium telluride quantum dot as fluorescent material, has the light stability of narrower emmission spectrum and Geng Gao.Utilize silica shell that Raman molecular and cadmium telluride quantum dot are separated, avoid generate energy transfer between the two, make Raman molecular and cadmium telluride quantum dot can well keep the spectral response curve of self.These structures have guaranteed strong, the good operating stability of the signal of optical encoding probe.
Description of drawings
Fig. 1 is the structural representation of the double mode optical encoding probe of the utility model.
Fig. 2 is to be the fluorescence spectrum of the double mode optical encoding probe solution of fluorescent material with the cadmium telluride quantum dot, and excitation wavelength is 400 nm.
Fig. 3 is with 5; (English full name is 5 to 5-dithio two (2-nitrobenzoic acid); 5-dithiobis (2-nitro-benzoic acid) is abbreviated as DTNB) molecule is the SERS spectrum of the double mode optical encoding probe particle of Raman molecular, excitation wavelength is 633 nm.
Fig. 4 is to be the SERS spectrum of the double mode optical encoding probe particle of Raman molecular with 4 Thiosalicylic acids (English full name is 4-mercaptobenzoic acid, is abbreviated as 4MBA) molecule, and excitation wavelength is 633nm.
Fig. 5 is to be the SERS spectrum of the double mode optical encoding probe particle of Raman molecular with 4MBA and DTNB mixture, and excitation wavelength is 633nm.
Have among the figure: the first layer nuclear 1, Raman molecular 2,3, the three layers of shell 4 of second layer shell.
Embodiment
Below in conjunction with accompanying drawing, the technical scheme of the utility model is carried out detailed explanation.
As shown in Figure 1, a kind of double mode optical encoding probe of the utility model, this probe adopts three-layer nuclear shell structure; The first layer nuclear 1 is gold nanorods, and second layer shell 3 is a silicon-dioxide, and the 3rd layer of shell 4 is cadmium telluride quantum dot; Second layer shell 3 is wrapped in the outside of the first layer nuclear 1; The 3rd layer of shell 4 pastes in the outside of second layer shell 3, and the outside surface of the first layer nuclear 1 is covered with Raman molecular 2, and Raman molecular 2 is by second layer shell 3 parcels.
The double mode optical encoding probe of this structure is the nanoparticle based on three-layer nuclear shell structure, and innermost layer has been examined to mark the gold nanorods of Raman molecular 2, its outside parcel silica shell, and outermost layer is a cadmium telluride quantum dot.This double mode optical encoding probe can produce fluorescence and SERS signal simultaneously under excitation light irradiation, possess fluorescence and SERS combined coding ability.
Further, described Raman molecular 2 pastes the outside surface of whole the first layer nuclear 1.When Raman molecular 2 pasted the outside surface of whole the first layer nuclear 1, Raman molecular 2 can provide stronger SERS signal.
Further, described cadmium telluride quantum dot pastes the outside surface of whole second layer shell 3.When cadmium telluride quantum dot pasted the outside surface of whole second layer shell 3, cadmium telluride quantum dot can provide stronger fluorescent signal.
In order to make double mode optical encoding probe have stronger code capacity, the diameter of cadmium telluride quantum dot has two or more.Raman molecular 2 comprises two or more.Raman molecular 2 contains phenyl ring molecule and sulphur atom.When the raman scattering cross section of Raman molecular 2 is big more, just can produce strong more Raman scattering signal.Usually the Raman molecular 2 that contains phenyl ring has bigger raman scattering cross section.Raman molecular 2 is to be easy to be adsorbed onto on the gold nanorods through self sulphur atom and the formation of the gold on gold nanorods gold-sulfide linkage, so Raman molecular 2 preferably contains sulphur atom.Raman molecular 2 preferred 4 Thiosalicylic acid molecules, 5, the one or any combination in two (2-nitrobenzoic acid) molecules of 5-dithio, 4 aminothiophenol molecules, 4 methoxybenzenethiol molecules or the 2 thionaphthol molecules.
If the double mode optical encoding probe of this structure has N the cadmium telluride quantum dot that diameter is different; M different types of Raman molecular 2, the number H=of so double mode optical encoding probe codified
.For example, adopt two kinds of Raman moleculars and a kind of cadmium telluride quantum dot totally 3 kinds of double mode optical encoding probes of coding material prepn.Two kinds of Raman moleculars are adopted as 5,5-dithio two (2-nitrobenzoic acids) (DTNB) with 4 Thiosalicylic acids (4MBA), quantum dot is the water cadmium telluride quantum dot.With the signal on the optical encoding probe in 3 binary codes (xxx) expression the utility model.From left to right, the first bit representation quantum dot; The second bit representation 4MBA; The 3rd bit representation DTNB; " 1 " expression has, and " 0 " expression does not have.Then actual obtainable sign indicating number has (100) (101) (110) (111) (010) (001) (011) totally 7 kinds of sign indicating numbers ((000) sign indicating number is meaningless); And adopt 3 kinds of emission wavelength quantum dots or 3 kinds of Raman moleculars separately; Because the influence of spectra overlapping; Usually actually can only obtain 3 kinds or 4 kinds of sign indicating numbers, it is very difficult to obtain 7 kinds of sign indicating numbers.
The preparation method of above-mentioned double mode optical encoding probe may further comprise the steps:
The original gold nanorods solution of step 1. preparation: at first cetyl trimethylammonium bromide solution and tetra chlorauric acid solution are mixed, add sodium borohydride solution again, stir, make gold seeds solution; In cetyl trimethylammonium bromide solution, add silver nitrate solution, tetra chlorauric acid solution, deionized water, ascorbic acid solution then successively, become colorless to mixing solutions, process growth solution; In growth solution, add gold seeds solution at last, make original gold nanorods solution;
The gold nanorods of step 2. preparation Raman molecular mark: with whizzer with the original gold nanorods solution of step 1 preparation carry out centrifugal after; In original gold nanorods solution, form deposition; Extract and deposition to add in the deionized water, make resolution of precipitate in deionized water, form gold nanorods solution; The ethanolic soln that will be dissolved with Raman molecular then adds in the gold nanorods solution, makes the gold nanorods of Raman molecular mark after the stirring;
Step 3. preparation gold nanorods and silicon-dioxide metal medium composite Nano ball solution: close silicon processing is carried out on the gold nanorods surface of the Raman molecular mark that at first makes through polymer electrolytic confrontation step 2; Gold nanorods with the Raman molecular mark is transferred in the spirituous solution then; In this spirituous solution, add ammoniacal liquor and tetraethoxy subsequently; This moment is in the gold nanorods surface growth layer of silicon dioxide of Raman molecular mark, thus formation gold nanorods and silicon-dioxide metal medium composite Nano ball solution;
The double mode optical encoding probe of step 4. preparation: the gold nanorods and the silicon-dioxide metal medium composite Nano ball solution metal medium composite Nano ball that at first step 3 are made carry out amido modified; Then amido modified gold nanorods and silicon-dioxide metal medium composite Nano ball solution are mixed stirring with cadmium telluride quantum dot solution; Cadmium telluride quantum dot is adsorbed to the surface of gold nanorods and silicon-dioxide metal medium composite Nano ball, makes double mode optical encoding probe.
Embodiment 1
With the gold nanorods is that SERS strengthens substrate, and with 5,5-dithio two (2-nitrobenzoic acid) (DTNB) molecule is the SERS affinity tag, is fluorescent material with the cadmium telluride quantum dot, prepares double mode optical encoding probe particle, and the preparation method comprises the steps:
The original gold nanorods solution of step 1. preparation: at first prepare gold seeds; At room temperature (promptly in 23 ~ 30 ℃ TR; Hereinafter is mentioned room temperature, and is identical therewith), 2.5mL 0.2M cetyl trimethylammonium bromide (abbreviation CTAB) solution is mixed with 1.5mL 1.0mM tetra chlorauric acid solution; Vigorous stirring also adds the iced sodium borohydride solution of 0.6mL 0.01M, stops after 2 minutes stirring promptly getting brown xanchromatic seed solution.Prepare growth solution then, in 50mL 0.2M CTAB solution, add following reagent under the room temperature successively: 2 ~ 4mL 4mM silver nitrate solution, 5mL 15mM tetra chlorauric acid solution, the 45mL deionized water slowly stirs.Adding 1.5mL ~ 3mL 0.08M xitix to solution subsequently becomes colorless.Add the 1mL seed solution at last, leave standstill 10 ~ 20min and promptly get original gold nanorods solution.About 15nm * the 45nm of gained gold nanorods size.
The gold nanorods aqueous solution of step 2. preparation Raman molecular mark: get the original gold nanorods solution of 5mL with 10000 rpm, 30 minutes centrifugal excessive reactant of once removing.Centrifugation is dispersed in the 5mL deionized water, adds 10 ~ 50 μ L 10mM DTNB ethanolic solns, more than the vigorous stirring 3h.
Step 3. preparation gold nanorods and silicon-dioxide metal medium composite Nano ball solution: the gold nanorods aqueous solution of the Raman molecular mark that step 2 is prepared is with 8000 rpm; 30 minutes once centrifugal; Precipitation is dissipated in 1 ~ 2mL 10mg/mL polypropylene amine hydrochloride (being abbreviated as " PAH ", molecular weight 15000) aqueous solution, slowly stirs behind the 1h with 8000 rpm; 30 minutes once centrifugal, and precipitation is dissipated in the 5mL deionized water.Add 1 ~ 2mL 25mg/mL Vinylpyrrolidone polymer (being abbreviated as " PVP ", molecular weight 8000) aqueous solution, with 8000 rpm, 30min is once centrifugal after the slow stirred overnight, and precipitation is dissipated in the 5mL absolute ethyl alcohol.Add the ammoniacal liquor of 300 ~ 500 μ L 25%, the positive tetraethyl orthosilicate of 15 ~ 30 μ L (being abbreviated as " TEOS ") promptly gets gold nanorods and silicon-dioxide metal medium composite Nano ball solution more than the solution stirring 6h.Eccentric cleaning is also collected the nanometer ball in the reaction solution.This nanometer ball is dispersed in the 5mL deionized water the most at last, the about 100nm of the diameter of nanometer ball.
4) the double mode optical encoding probe of preparation: at first make particle show as positive polarity at gold nanorods and silicon-dioxide metal medium composite Nano ball finishing amino; Add 20 ~ 100 μ L, 10% polymine (being abbreviated as " PEI ", the molecular weight 10000) aqueous solution in 1mL gold nanorods and the silicon-dioxide metal medium composite Nano ball solution and slowly stir 1h.With 6500 rpm, eccentric cleaning was 3 times in 10 minutes, and gold nanorods and the silicon-dioxide metal medium composite Nano ball with amination is dispersed in the 1mL deionized water at last.Water cadmium telluride quantum dot part is Thiovanic acid or thiohydracrylic acid; In the aqueous solution, show as electronegativity; 0.5 ~ 1mL cadmium telluride quantum dot aqueous solution is mixed more than the also slow 3h of stirring with amido modified gold nanorods and the silicon-dioxide metal medium composite Nano ball solution of above-mentioned 1mL, and eccentric cleaning is removed excessive cadmium telluride quantum dot and is promptly got the double mode optical encoding probe that has fluorescence and SERS signal simultaneously.
The fluorescence of this double mode optical encoding probe is surveyed through XRF, and excitation wavelength is 400 nm.When surveying SERS spectrum, this optical encoding probe is dripped on sheet glass, and be fixed on photometry spectrum on the confocal Raman spectra appearance, laser source is the Argon of 633 nm, and the irradiation power on the sample is 2.3 mW, and be 60 s integral time.The existing fluorescence of this optical encoding probe (Fig. 2) has the very high SERS signal (Fig. 3) of SNR again.Through quantum dot or the different SERS tagged molecule of changing different emission wavelengths, can give this probe different optical properties, realize the function of fluorescence and SERS combined coding.The gained sign indicating number is (101) among this embodiment.If medium/metal composite nano-granule sub-surface is not adsorbed cadmium telluride quantum dot, then obtain (001) sign indicating number.If the gold nanorods surface does not connect DTNB, metal medium composite nanoparticle surface adsorption cadmium telluride quantum dot then gets (100) sign indicating number simultaneously.
Embodiment 2
With the gold nanorods is that SERS strengthens substrate, is the SERS affinity tag with 4 Thiosalicylic acids (4MBA) molecule, is fluorescent material with the cadmium telluride quantum dot, prepares double mode optical encoding probe particle, and this method comprises the steps:
Step 1: prepare original gold nanorods solution.Prepare original gold nanorods solution according to consumption among the embodiment 1 and step.
Step 2: the gold nanorods surface connects the 4MBA molecule.Get the original gold nanorods solution of 5mL with 10000 rpm, 30 minutes centrifugal once to remove excessive reactant.Centrifugation is dispersed in the 5mL deionized water, adds 10 ~ 50 μ L 10mM 4MBA ethanolic solns, more than the vigorous stirring 3h.
Step 3: connected the gold nanorods surface parcel silicon-dioxide of 4MBA molecule, preparation metal medium composite Nano ball solution.With 8000 rpm, 30 minutes once centrifugal with the solution of step 2 preparation, and precipitation is dissipated in 1 ~ 2mL 10mg/mL PAH aqueous solution, slowly stirs behind the 1h with 8000 rpm, and 30 minutes once centrifugal, and precipitation is dissipated in the 5mL deionized water.Add 1 ~ 2mL 25mg/mL PVP aqueous solution, with 8000 rpm, 30min is once centrifugal after the slow stirred overnight, and precipitation is dissipated in the 5mL absolute ethyl alcohol.Add the ammoniacal liquor of 300 ~ 500 μ L 25%, promptly get metal medium composite Nano ball more than 15 ~ 30 μ L TEOS solution stirring 6h, eccentric cleaning is also collected the nanometer ball in the reaction solution.This nanometer ball is dispersed in the 5mL deionized water the most at last, the about 100nm of nanometer ball diameter.
4) metal medium composite nanoparticle surface electrostatic absorption cadmium telluride quantum dot.Add 20 ~ 100 μ L, the 10% PEI aqueous solution in the 1mL metal medium composite Nano ball aqueous solution and slowly stir 1h.With 6500 rpm, eccentric cleaning was 3 times in 10 minutes, and the metal medium composite Nano ball with amination is dispersed in the 1mL deionized water at last.0.5 ~ 1mL cadmium telluride quantum dot aqueous solution is mixed with the amido modified metal medium composite Nano ball solution of above-mentioned 1mL more than the also slow 3h of stirring, and eccentric cleaning is removed excessive cadmium telluride quantum dot and is promptly got the double mode optical encoding probe that has fluorescence and SERS signal simultaneously.
The fluorescent signal of this probe is seen Fig. 2, and the SERS signal is seen Fig. 4.The fluorescence of this double mode optical encoding probe is surveyed through XRF, and excitation wavelength is 400 nm.When surveying SERS spectrum, this optical encoding probe is dripped on sheet glass, and be fixed on photometry spectrum on the confocal Raman spectra appearance, laser source is the Argon of 633 nm, and the irradiation power on the sample is 2.3 mW, and be 60 s integral time.The gained sign indicating number is (110) among this embodiment.If cadmium telluride quantum dot is not adsorbed on metal medium composite Nano ball surface, then obtain (010) sign indicating number.If the gold nanorods surface does not connect 4MBA, metal medium composite nanoparticle surface adsorption cadmium telluride quantum dot then gets (100) sign indicating number simultaneously.
Embodiment 3
With the gold nanorods is that SERS strengthens substrate, is the SERS affinity tag with 4MBA and DTNB mixture, is fluorescent material with the cadmium telluride quantum dot, prepares double mode optical encoding probe particle, and this method comprises the steps:
Step 1: prepare original gold nanorods solution.Prepare original gold nanorods solution according to consumption among the embodiment 1 and step.
Step 2: the gold nanorods surface connects 4MBA and two kinds of molecules of DTNB.Get the original gold nanorods solution of 5mL with 10000 rpm, 30 minutes centrifugal excessive reactant of once removing.Centrifugation is dispersed in the 5mL deionized water, adds the ethanolic soln that 10 ~ 50 μ L contain 5mM 4MBA and 5mM DTNB, more than the vigorous stirring 3h.
Step 3: the gold nanorods surface parcel silicon-dioxide that has connected Raman molecular prepares the metal medium composite nanoparticle.With 8000 rpm, 30 minutes once centrifugal with the solution of step 2 preparation, and precipitation is dissipated in 1 ~ 2mL 10mg/mL PAH aqueous solution, slowly stirs behind the 1h with 8000 rpm, and 30 minutes once centrifugal, and precipitation is dissipated in the 5mL deionized water.Add 1 ~ 2mL 25mg/mL PVP aqueous solution, with 8000 rpm, 30min is once centrifugal after the slow stirred overnight, and precipitation is dissipated in the 5mL absolute ethyl alcohol.Add the ammoniacal liquor of 300 ~ 500 μ L 25%, promptly get metal medium composite Nano ball more than 15 ~ 30 μ L TEOS solution stirring 6h, eccentric cleaning is also collected the nanometer ball in the reaction solution.This nanometer ball is dispersed in the 5mL deionized water the most at last, the about 100nm of nanometer ball diameter.
Step 4: metal medium composite nanoparticle surface electrostatic absorption cadmium telluride quantum dot.Add 20 ~ 100 μ L, the 10% PEI aqueous solution in the 1mL metal medium composite nanoparticle aqueous solution and slowly stir 1h.With 6500 rpm, eccentric cleaning was 3 times in 10 minutes, and the metal medium composite nanoparticle with amination is dispersed in the 1mL deionized water at last.The metal medium composite Nano ball solution of 0.5 ~ 1mL cadmium telluride quantum dot aqueous solution and above-mentioned 1mL amination modification is mixed more than the also slow 3h of stirring, and eccentric cleaning is removed excessive cadmium telluride quantum dot and is promptly got the double mode optical encoding probe that has fluorescence and SERS signal simultaneously.
This fluorescence probe signal is seen Fig. 2, and the SERS signal is seen Fig. 5.The fluorescence of this double mode optical encoding probe is surveyed through XRF, and excitation wavelength is 400 nm.When surveying SERS spectrum, this optical encoding probe is dripped on sheet glass, and be fixed on photometry spectrum on the confocal Raman spectra appearance, laser source is the Argon of 633 nm, and the irradiation power on the sample is 2.3 mW, and be 60 s integral time.The gained sign indicating number is (111) among this embodiment.If cadmium telluride quantum dot is not adsorbed on metal medium composite Nano ball surface, then obtain (011) sign indicating number.
From the spectral line of Fig. 2 to Fig. 5, can find out: signal absolute strength numerical value is very big, spectral line is very level and smooth, SNR is very high, and the probe of this structure has the strong characteristic of signal.
The above only is the preferred implementation of the utility model; Be noted that for those skilled in the art; Under the prerequisite that does not break away from the utility model principle; Can also make some improvement and retouching, these improvement and retouching also should be regarded as the protection domain of the utility model.
Claims (4)
1. a double mode optical encoding probe is characterized in that, this probe adopts three-layer nuclear shell structure; The first layer nuclear (1) is gold nanorods, and second layer shell (3) is a silicon-dioxide, and the 3rd layer of shell (4) is cadmium telluride quantum dot; Second layer shell (3) is wrapped in the outside of the first layer nuclear (1); The 3rd layer of shell (4) pastes in the outside of second layer shell (3), and the outside surface of the first layer nuclear (1) is covered with Raman molecular (2), and Raman molecular (2) is wrapped up by second layer shell (3).
2. according to the described double mode optical encoding probe of claim 1, it is characterized in that described Raman molecular (2) pastes the outside surface of whole the first layer nuclear (1).
3. according to the described double mode optical encoding probe of claim 1, it is characterized in that described cadmium telluride quantum dot pastes the outside surface of whole second layer shell (3).
4. according to the described double mode optical encoding probe of claim 1, it is characterized in that the diameter of described cadmium telluride quantum dot has two or more.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105482803A (en) * | 2015-11-26 | 2016-04-13 | 东南大学 | Fluorescence-SERS dual-mode super-resolution imaging probe and its preparation method and use method |
| CN105817617A (en) * | 2016-05-24 | 2016-08-03 | 中国科学院理化技术研究所 | Gold nanorod/silicon dioxide/carbon point nanometer hybrid material and preparation method and application thereof |
| CN112326623A (en) * | 2020-10-23 | 2021-02-05 | 中国人民解放军军事科学院军事医学研究院 | Silicon-core-silver-shell composite nano label marked by double-layer Raman molecules, preparation method and immunochromatography application |
| WO2022143555A1 (en) * | 2020-12-31 | 2022-07-07 | Tcl科技集团股份有限公司 | Method for preparing core-shell quantum dots and core-shell quantum dots |
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2012
- 2012-01-12 CN CN2012200115976U patent/CN202499839U/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105482803A (en) * | 2015-11-26 | 2016-04-13 | 东南大学 | Fluorescence-SERS dual-mode super-resolution imaging probe and its preparation method and use method |
| CN105817617A (en) * | 2016-05-24 | 2016-08-03 | 中国科学院理化技术研究所 | Gold nanorod/silicon dioxide/carbon point nanometer hybrid material and preparation method and application thereof |
| CN105817617B (en) * | 2016-05-24 | 2018-01-30 | 中国科学院理化技术研究所 | A kind of nano-hybrid material of gold nanorods/silicon/carbon dioxide point and its preparation method and application |
| CN112326623A (en) * | 2020-10-23 | 2021-02-05 | 中国人民解放军军事科学院军事医学研究院 | Silicon-core-silver-shell composite nano label marked by double-layer Raman molecules, preparation method and immunochromatography application |
| CN112326623B (en) * | 2020-10-23 | 2024-05-14 | 中国人民解放军军事科学院军事医学研究院 | Silicon core silver shell composite nano-tag with double-layer Raman molecular marker, preparation method and immunochromatography application |
| WO2022143555A1 (en) * | 2020-12-31 | 2022-07-07 | Tcl科技集团股份有限公司 | Method for preparing core-shell quantum dots and core-shell quantum dots |
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| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20121024 Termination date: 20140112 |