CN202471719U

CN202471719U - Luteinizing hormone rapid detection reagent strip

Info

Publication number: CN202471719U
Application number: CN2012200927377U
Authority: CN
Inventors: 陈国琴; 应旭霞; 周文良
Original assignee: HANGZHOU LONGJI BIOLOGICAL TECHNOLOGY CO LTD
Current assignee: HANGZHOU LONGJI BIOLOGICAL TECHNOLOGY CO LTD
Priority date: 2012-03-13
Filing date: 2012-03-13
Publication date: 2012-10-03
Anticipated expiration: 2022-03-13

Abstract

The utility model belongs to the technical field of immunodetection and relates to a reagent strip, in particular to a luteinizing hormone rapid detection reagent strip, which comprises a holder, sample pads, colloidal gold pieces, a cellulose nitrate film and an absorbent pad. From a sample adding end, the sample pads, the colloidal gold pieces, the cellulose nitrate film and the absorbent pad are successively adhered on the holder. Two ends of the cellulose nitrate film are respectively connected with the colloidal gold pieces and the absorbent pad in an overlap mode. A detection area and a control area are arranged on the cellulose nitrate film. An alpha LH monoclonal resistance antibody and a beta LH monoclonal resistance antibody are enveloped in the detection area and a goat-anti-mouse polyclonal antibody is enveloped in the control area. Therefore, the luteinizing hormone rapid detection reagent strip has the advantages of simple operation, rapid reaction, high sensibility, and strong specificity and being suitable for field detection and further has the advantages of short production cycle, high yield, low cost and the like. Therefore, the luteinizing hormone rapid detection reagent strip has a good application prospect in the technical field of immunodetection.

Description

A kind of luteinizing principle quick detection reagent bar

Technical field

The utility model belongs to technical field of immunoassay, relates to a kind of reagent strip, relates in particular to a kind of luteinizing principle quick detection reagent bar.

Background technology

At present, a lot of about the assay method of LH both at home and abroad, mainly contain basal body temperature (BBT), the scoring of uterine neck mucus, blood LH mensuration, the ultra monitoring of B, endometrium biopsy etc.

The principle of BBT monitoring ovulation is the pyrogenic action according to progesterone, and progesterone secretion increase causing BBT in ovulation back raises.Its biggest advantage is easy, no wound, and is also not time-consuming.Shortcoming is that the correlativity of biphasic or bipolar type BBT and ovulation is relatively poor, and what ovulate in the 1d before and after the BBT minimum point only accounts for 54.5 %, removes the interference of factors such as this also receives sleep, takes medicine, diet, disease.And BBT judges ovulation through Retrospective analysis, so predicting ovulation, instruct to become pregnant and have little significance, visible this kind method only can be as clinical reference index.

In ovulatory cycle, the uterine neck mucus receives the effect of estrogen and progestogen, and its physics and chemical characteristic are cyclical variation, and it is the window of ovarian follicle generation E2, and increase the onset of ovulation, and the scoring of uterine neck mucus is increased.There are the scoring of report uterine neck mucus and the accuracy rate of ovulation to be merely 53.3%, and receive factor affecting such as medicine, inflammation, and need come hospital to operate at every turn by medical practitioner.In recent years have the scholar to replace loaded down with trivial details uterine neck mucus scoring with the saliva fernlike crystal, both coincidence rates of inspection are 100%, but because of the accuracy rate of uterine neck mucus and ovulation is lower, this kind method also only supplies clinical reference.

It is the goldstandard of predicting ovulation that blood LH measures.In ovulatory cycle was arranged, the early stage blood LH of ovarian follicle level was lower, about 2～30IU/L, and late follicular phase reaches 20～40IU/L, reaches peak 40～200IU/L before the ovulation, claims the LH peak value.Ovulation occurs in 24～48h behind the blood LH peak value, and maximum confidence is more than 90 %, but owing to need repeatedly blood sampling, expensive and can not in time obtain the result and instruct application, it is restricted in clinical practice.

The endometrium biopsy is an another kind of method of judging ovulation, should get biopsy at the 6h of menstrual onset.Its principle is that ovulation back progesterone secretion increases, and makes proliferative phase of endometrium be converted into secretory endometrium.Yet the endometrium biopsy is a kind of invasive surgery operation after all, and certain misery is arranged, and expense is high, and clinical effect only slightly is superior to BBT, serum progesterone mensuration, blood LH mensuration, and therefore carrying out the endometrium biopsy should have certain indication.For long-term chronic anovulation women, the endometrium biopsy helps to make a definite diagnosis the long and endometrial tumors of endometrial hyperplasia, but and guiding treatment.

The transvaginal sonography dynamic monitoring is the method that up-to-date and the most practical monitoring is ovulated, and also is the method that unique metamorphosis from external Direct observation ovarian follicle is diagnosed ovulation.But need by the technical skill personnel operation, inspection charge is more expensive.And because of the big minor swing of graaffian follicle between the individuality big (17.35～28.59 mm); And everyone has the follicular development type of oneself; Ovulate behind the appearance graaffian follicle and betide in 0.64～4.28d; So list comes the definite time of predicting ovulation not accurate enough with FD, the associating additive method can be confirmed ovulation more accurately.

The luteinizing principle quick detection reagent has easy to carry, does not need equipment, and operating personnel need not technical training, but execute-in-place can provide advantages such as result in 3～5 minutes, was fit to very much sample in enormous quantities is carried out the work of field screening.

(human luteinizing hormone, one of promoting sexual gland hormone of hLH) being secreted by anterior pituitary is a kind of glycoprotein hormones to luteinizing principle, has very strong immunocompetence.The menstrual cycle that ovary is kept in the collaborative FSH acting in conjunction of LH in female body, cause ovulation and corpus luteum to form.Clinical in detecting the LH peak value; Confirm initial peak of LH and the time that reaches summit; Can reach the purpose of predicting ovulation; Hold the best opportunity become pregnant and practise contraception effectively, the women of infertility, family planning, prenatal and postnatal care and safe period is played good directive function, can also assist to judge the menopathy cause of disease.

Promoting sexual gland hormone FSH, LH are glycoprotein hormoneses, all are made up of α, two subunits of β.The α subunit of the two is identical, all be made up of 89 amino acid, but the β subunit is different, can different with target cell specifically receptors bind, and through bringing out second messenger's formation, impel follicular development, ovulation and corpus luteum to form.

Early stage in follicular development, nucleus arcuatus hypothalami pulsed ground secretion gonadotropin-releasing hormone (GRH) (GnRH), this hormone flows to anterior pituitary through portal vein capillary system, to regulate the secretion of pituitary gonadotropic hormone.This moment, follicle-stimulating hormone (FSH) (FSH) level raise, and luteinizing principle (LH) is in low-level, FSH/LH＞1.This moment, LH was the pulse mode secretion on a constant secretion basis, and 1 ~ 2 hour recurrence interval, LH impulsion follicular phase is at 5 ~ 10IU/L, and luteal phase is low than follicular phase.FSH stimulates ovarian follicular growth growth in the ovary, and estrogen secretion is arranged when FD reaches 8-10mm, and along with growth estradiol (E2) value of ovarian follicle slowly raises; Be negative feedback to hypothalamus and anterior pituitary; Suppress FSH and further secrete, ripe to dominant follicle further growth follicular phase in evening, after the E2 secretion is accelerated to reach its peak value; Then being positive feedback to hypothalamus makes its pulsatile secretion and discharges GnRH; Cause the LH secretion and be the peak value increase, FSH also correspondingly raises simultaneously, but FSH/LH＜1.Begin to raise in the LH secretion, be initial peak when doubling than base value; The LH secretion was elevated to summit about 16 hours from initial peak, and the LH peak value can reach about 70IU/L in the urine, and mxm. can be kept 24 hours, peak value ovulation in about 10 hours later.The then hurried decline of progesterone (p) rising E2 in the blood plasma when the LH peak value.Ovulation back corpus luteum forms, E2 second peak value in the formation cycle that also slightly raises, and FSH and LH then begin to descend in the blood plasma.After this corpus luteum atrophy gradually, in about 14 days of corpus luteum life-span, female, progestational hormone secretion descends and causes menstrual onset, and makes ovarian follicle begin to grow and get into the next ovarian follicle cycle.Can judge the time that it's too late ovulates that has of its ovulation clinically through LH peak value in the detection urine.

Summary of the invention

The purpose of the utility model provides a kind of luteinizing principle quick detection reagent bar, simple to operate, reaction fast, high, the high specificity of susceptibility and be fit to on-the-spot the detection, but also have with short production cycle, output is high, low cost and other advantages.

In order to solve the problems of the technologies described above, the utility model is able to solve through following technical proposals:

A kind of luteinizing principle quick detection reagent bar; Comprise holder, sample pad, the collaurum scraps of paper and adsorptive pads; Begin to be pasted with successively sample pad, the collaurum scraps of paper and adsorptive pads from the application of sample end on the holder, also comprise nitrocellulose filter, nitrocellulose filter is located between the collaurum scraps of paper and the adsorptive pads; The two ends of nitrocellulose filter overlap each other with adsorptive pads with the collaurum scraps of paper respectively and are connected; Detection zone and check plot are arranged on the nitrocellulose filter, and detection zone encapsulates anti-α-LH monoclonal antibody and anti-β-LH monoclonal antibody, and the check plot encapsulates the sheep anti mouse polyclonal antibody.

A kind of luteinizing principle quick detection reagent bar of the utility model, simple to operate, reaction fast, high, the high specificity of susceptibility and be fit to on-the-spot the detection, but also have with short production cycle, output is high, low cost and other advantages.Have a good application prospect in technical field of immunoassay.

Description of drawings

Fig. 1 is the structural representation of the said a kind of luteinizing principle quick detection reagent bar of the utility model.

Embodiment

The utility model is described in further detail with embodiment below in conjunction with accompanying drawing 1.

Embodiment

A kind of luteinizing principle quick detection reagent bar; As shown in Figure 1, comprise holder 5, sample pad 1, the collaurum scraps of paper 2 and adsorptive pads 4, begin to be pasted with successively sample pad 1, the collaurum scraps of paper 2 and adsorptive pads 4 from the application of sample end on the holder; Also comprise nitrocellulose filter 3; Nitrocellulose filter 3 is located between the collaurum scraps of paper 2 and the adsorptive pads 4, and the two ends of nitrocellulose filter 3 overlap each other with adsorptive pads 4 with the collaurum scraps of paper 2 respectively and are connected, and detection zone and check plot are arranged on the nitrocellulose filter 3; Detection zone encapsulates anti-α-LH monoclonal antibody and anti-β-LH monoclonal antibody, and the check plot encapsulates the sheep anti mouse polyclonal antibody.

Utilize the principle of colloidal gold immunochromatographimethod, the detection zone on nitrocellulose filter 3 encapsulates anti-β-LH monoclonal antibody, and the check plot encapsulates the sheep anti mouse polyclonal antibody.When tested antigenic substance (being luteinizing principle) is arranged in the determinand; Antigenic substance in the determinand will form the Ag-Ab-Au compound with the anti-α-LH monoclonal antibody antibody of mark collaurum; When the avtive spot of antibody will be occupied by the medicine in the sample solution in this compound, simultaneously again with the T line on drug antibody combine; So when the luteinizing principle content in the sample reaches 40mIU/mL when above, the T line colour developing on the detectable bar can be judged to be the positive.Otherwise when luteinizing principle content in the sample below 40mIU/mL or during noresidue, the T line on the detectable bar does not develop the color, and is judged to be feminine gender.Simple to operate, reaction fast, high, the high specificity of susceptibility and be fit to on-the-spot the detection, but also have with short production cycle, output is high, low cost and other advantages.Have a good application prospect in technical field of immunoassay.

In a word, the above is merely the preferred embodiment of the utility model, and all equalizations of being done according to the utility model claim change and modify, and all should belong to the covering scope of the utility model patent.

Claims

1. luteinizing principle quick detection reagent bar; Comprise holder (5), sample pad (1), the collaurum scraps of paper (2) and adsorptive pads (4); Begin to be pasted with successively sample pad (1), the collaurum scraps of paper (2) and adsorptive pads (4) from the application of sample end on the holder; It is characterized in that: also comprise nitrocellulose filter (3); Nitrocellulose filter (3) is located between the collaurum scraps of paper (2) and the adsorptive pads (4), and the two ends of nitrocellulose filter (3) overlap each other with adsorptive pads (4) with the collaurum scraps of paper (2) respectively and are connected, and nitrocellulose filter has detection zone and check plot on (3); Detection zone encapsulates anti-α-LH monoclonal antibody and anti-β-LH monoclonal antibody, and the check plot encapsulates the sheep anti mouse polyclonal antibody.