CN202403798U - Obtaining device for multispectral microscopic image - Google Patents

Obtaining device for multispectral microscopic image Download PDF

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Publication number
CN202403798U
CN202403798U CN2012200334668U CN201220033466U CN202403798U CN 202403798 U CN202403798 U CN 202403798U CN 2012200334668 U CN2012200334668 U CN 2012200334668U CN 201220033466 U CN201220033466 U CN 201220033466U CN 202403798 U CN202403798 U CN 202403798U
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CN
China
Prior art keywords
adjustable band
camera
band pass
pass filter
adjustable
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Withdrawn - After Issue
Application number
CN2012200334668U
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Chinese (zh)
Inventor
陈木旺
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Maike Aodi Industry Group Co Ltd
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Maike Aodi Industry Group Co Ltd
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Priority to CN2012200334668U priority Critical patent/CN202403798U/en
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Publication of CN202403798U publication Critical patent/CN202403798U/en
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Withdrawn - After Issue legal-status Critical Current

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Abstract

The utility model discloses an obtaining device for a multispectral microscopic image, comprising a microscope, an adjustable band-pass optical filter device and a camera which are arranged on the same optical axis, wherein the camera is electrically interconnected with a computer, the adjustable band-pass optical filter device is connected with a motor, the rotation or swinging of the motor is controlled by the computer via a controller, and then the swinging of the adjustable band-pass optical filter device in an optical path between a light source of the microscope and the camera is driven; and the adjustable band-pass optical filter device is an adjustable band-pass optical filter leaf or two adjustable band-pass optical filter leafs assembled at a certain included angle. Compared with the prior art, the obtaining device for a multispectral microscopic image disclosed by the utility model has the following beneficial effects that: 1) the overall structure of the obtaining device is simple, implementation is easy and operation is convenient; 2) the spectral transmission rate of the obtaining device is high; 3) the included angle between the adjustable band-pass optical filter leaf and the optical axis can be continuously changed in a range from 0 to 60 degrees, so that the expansibility of the obtaining device is good, and the wavelength is replaceable; and 4) the production cost of the whole system is low.

Description

A kind of deriving means of multispectral micro-image
Technical field
The utility model belongs to the microscopy field, is specifically related to a kind of deriving means of multispectral micro-image.
Background technology
Multispectral microscope has obtained one-dimension information than simple microscope more; It is spectral information; Quantitative test more accurately can be provided, thereby have important use value in fields such as clinical medicine, pathology, histology, immunohistochemistry, pharmaceutical engineering, materialogies.
Present multispectral microscope generally adopts light-splitting devices such as grating, electric tuning filtering device (LCTF or AOTF), color filter to obtain spectral information; Adopt grating to obtain spectral information like Chinese patent ZL200410017031.4; Chinese patent ZL200310111610.0 adopts the electric tuning filtering device to obtain spectral information; Though these two kinds of methods can obtain the micro-image of continuous high spectral resolution, cost is higher.
Patent WO2008074019A2 then discloses a kind of method that adopts color filter array, can obtain the multispectral data of limited several wave bands, though compact conformation can reduce spatial resolution; Wave band is many more, and it is many more that spatial resolution reduces, and after color filter array confirmed, its spectral band had also just been confirmed, can not arbitrarily change, can only be special-purpose, and expansion is not strong.
Summary of the invention
The technical matters that the utility model will solve is: overcome the deficiency of prior art, the deriving means of the multispectral micro-image that a kind of spatial resolution is high, cost is low is provided.
The utility model solves the technical scheme that its technical matters adopted: a kind of deriving means of multispectral image; Comprise computing machine, microscope, camera; It is characterized in that: said computing machine and said camera electrically connect each other; The logical filtering apparatus of one adjustable band is set between said microscope, the camera, and said microscope, camera, the logical filtering apparatus three of adjustable band are with the optical axis setting; The logical filtering apparatus of said adjustable band connects a telecontrol equipment, and said telecontrol equipment electrically connects said computing machine; Said camera, telecontrol equipment are by said computing machine synchro control.
Preferably; Said telecontrol equipment comprises a controller, a motor; Said motor connects with said computer-electrical through said controller; Send instruction via controller control motor by computing machine and rotate or swing, drive in the logical light path of filtering apparatus between said microscopical light source and said camera of said adjustable band and swing.
Preferably, the logical filtering apparatus of said adjustable band is an adjustable band pass filter; Said adjustable band pass filter is driven by telecontrol equipment, swings in the light path between said microscopical light source and said camera, can obtain the view data of different spectrum at said camera place.
Preferably, the angle between said adjustable band pass filter and the optical axis is an incident angle, is 0 °-60 °, can continuously change.
Preferably, described adjustable band pass filter is a narrow band pass filter, and the spectrum of its transmission is relevant with incident angle.
Preferably, the logical filtering apparatus of said adjustable band comprises two identical adjustable band pass filters, and described two adjustable band pass filter assemblings in a certain angle make both curves of spectrum that certain coincidence arranged; Described two adjustable band pass filters are driven by telecontrol equipment, swing in the light path between said microscopical light source and said camera, can obtain the view data of different spectrum at said camera place.
Preferably, the angle between said two adjustable band pass filters is adjustable; Angle is big more, and the bandwidth after the combination is narrow more.
Compared with prior art, the beneficial effect of the utility model is: 1) one-piece construction of the deriving means of the multispectral image of the utility model is simple, implements easily and handled easily; 2) spectral transmittance of the deriving means of this multispectral image is high; 3) angle between adjustable band pass filter and the optical axis can continuously change in 0 ° of-60 ° of scope, and perhaps the angle between two adjustable band pass filters is adjustable, so the expansion of the utility model is good, and wavelength is replaceable; 4) cost of manufacture of total system is lower.
Description of drawings
Fig. 1 is the structural representation of the utility model embodiment one;
Fig. 2 is the structural representation of the utility model embodiment two;
Fig. 3 is the curve of spectrum of adjustable band pass filter 21 among the utility model embodiment two;
Fig. 4 is the curve of spectrum of adjustable band pass filter 22 among the utility model embodiment two;
Fig. 5 is the bandwidth synoptic diagram (dash area) after adjustable band pass filter 21 and 22 makes up among the utility model embodiment two.
Embodiment
Below in conjunction with accompanying drawing embodiment, the utility model done further describing.
Embodiment one
As shown in Figure 1, a kind of deriving means of multispectral image comprises computing machine 6, microscope 1, camera 5, adjustable band pass filter 2, and said microscope 1, camera 5, adjustable band pass filter 2 threes are with the optical axis setting; Between adjustable band pass filter 2 installation microscopes 1 and the camera 5; And be fixed on the motor 3; The angle that under motor 3 drives, changes adjustable band pass filter 2 and optical axis is an incident angle; Motor 3 is electrically connected with controller 4, and controller 4 is electrically connected with computing machine 6 respectively with camera 5, by the synchronous working of computing machine 6 control cameras 5 and motor 3.
The image of section after microscope 1 amplifies after adjustable band pass filter 2 filters, has only specific spectrum picture to be imaged on the camera 5, can be obtained the view data of a certain wave band by the signal of computing machine 6 acquisition camera 5; Drive adjustable band pass filter 2 by computing machine 6 through controller 4 drive motor 3 again and forward another angle to; The signal of acquisition camera 5 simultaneously can obtain the view data of another wave band, and the rest may be inferred; Computing machine 6 control motors 3 rotate; Drive the different angle of adjustable band pass filter 2 rotation one sequences, and the signal of acquisition camera 5, a sequence multispectral image can be obtained.
Described microscope 1 is transmission microscopy or fluorescent microscope, or is infinity chromatic aberration correction system (CCIS); Described camera 5, preferably digital, black and white camera; Described motor 3, or be stepper motor, or for having the motor of scrambler; Described controller 4 is drivers supporting with motor 2; Described motor 3 drives 2 swings of adjustable band pass filter or rotation, and its incident angle is changed between 0 °-60 °; Described adjustable band pass filter 2 can also constitute an integral body with motor 3, can be used as an annex of microscope 1, can fast existing simple microscope be upgraded to multispectral microscope.
Embodiment two
Like Fig. 2,3,4, shown in 5,, can as a whole use be done in two identical adjustable band pass filters 21 and 22 θ placements in a certain angle and fixing in order to obtain the more spectrum of arrowband.Like this; Adjustable band pass filter 21 and adjustable band pass filter 22 are because of existing angle theta; The curve of spectrum that sees through separately has certain skew, and respectively like Fig. 3 and shown in Figure 4, its lap is exactly the bandwidth after the combination; Than narrower, the as shown in Figure 5 dash area of single adjustable band pass filter 21 or 22.Bandwidth after the combination depends on angle theta, and θ is big more, and bandwidth is narrow more, but can not be excessive, otherwise will cause the curve of spectrum of two adjustable band pass filters 21 and 22 not have overlapping and all end.Other part is identical with embodiment one.
The above; It only is the preferred embodiment of the utility model; Be not to be the restriction of the utility model being made other form, any professional and technical personnel of being familiar with possibly utilize the technology contents of above-mentioned announcement to change or be modified as the equivalent embodiment of equivalent variations.But every the utility model technical scheme content that do not break away to any simple modification, equivalent variations and remodeling that above embodiment did, still belongs to the protection domain of the utility model technical scheme according to the technical spirit of the utility model.

Claims (7)

1. the deriving means of a multispectral image; Comprise computing machine, microscope, camera; It is characterized in that: said computing machine and said camera electrically connect each other; The logical filtering apparatus of one adjustable band is set between said microscope, the camera, and said microscope, camera, the logical filtering apparatus three of adjustable band are with the optical axis setting; The logical filtering apparatus of said adjustable band connects a telecontrol equipment, and said telecontrol equipment electrically connects said computing machine; Said camera, telecontrol equipment are by said computing machine synchro control.
2. the deriving means of multispectral image according to claim 1; It is characterized in that: said telecontrol equipment comprises a controller, a motor; Said motor connects with said computer-electrical through said controller; Send instruction via controller control motor by computing machine and rotate or swing, drive in the logical light path of filtering apparatus between said microscopical light source and said camera of said adjustable band and swing.
3. the deriving means of multispectral image according to claim 1 and 2 is characterized in that: the logical filtering apparatus of said adjustable band is an adjustable band pass filter; Said adjustable band pass filter is driven by telecontrol equipment, swings in the light path between said microscopical light source and said camera, can obtain the view data of different spectrum at said camera place.
4. the deriving means of multispectral image according to claim 3, it is characterized in that: the angle between said adjustable band pass filter and the optical axis is an incident angle, is 0 °-60 °, can continuously change.
5. the deriving means of multispectral image according to claim 4, it is characterized in that: described adjustable band pass filter is a narrow band pass filter, and the spectrum of its transmission is relevant with incident angle.
6. the deriving means of multispectral image according to claim 1 and 2; It is characterized in that: the logical filtering apparatus of said adjustable band comprises two identical adjustable band pass filters; Described two adjustable band pass filter assemblings in a certain angle make both curves of spectrum that certain coincidence arranged; Described two adjustable band pass filters are driven by telecontrol equipment, swing in the light path between said microscopical light source and said camera, can obtain the view data of different spectrum at said camera place.
7. the deriving means of multispectral image according to claim 6, it is characterized in that: the angle between said two adjustable band pass filters is adjustable; Angle is big more, and the bandwidth after the combination is narrow more.
CN2012200334668U 2012-02-02 2012-02-02 Obtaining device for multispectral microscopic image Withdrawn - After Issue CN202403798U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN2012200334668U CN202403798U (en) 2012-02-02 2012-02-02 Obtaining device for multispectral microscopic image

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN2012200334668U CN202403798U (en) 2012-02-02 2012-02-02 Obtaining device for multispectral microscopic image

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CN202403798U true CN202403798U (en) 2012-08-29

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102589694A (en) * 2012-02-02 2012-07-18 麦克奥迪实业集团有限公司 Device for acquiring multispectral microscopic image
CN110114645A (en) * 2016-12-27 2019-08-09 优鲁格斯股份有限公司 Hyper spectral Imaging when observed object is static
CN110189819A (en) * 2019-04-08 2019-08-30 天津国科医工科技发展有限公司 A kind of multispectral motion control method for fluorescence microscopy scope

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102589694A (en) * 2012-02-02 2012-07-18 麦克奥迪实业集团有限公司 Device for acquiring multispectral microscopic image
CN102589694B (en) * 2012-02-02 2014-02-26 麦克奥迪实业集团有限公司 Device for acquiring multispectral microscopic image
CN110114645A (en) * 2016-12-27 2019-08-09 优鲁格斯股份有限公司 Hyper spectral Imaging when observed object is static
CN110114645B (en) * 2016-12-27 2021-11-26 优鲁格斯股份有限公司 Hyperspectral imaging while the viewed object is stationary
CN110189819A (en) * 2019-04-08 2019-08-30 天津国科医工科技发展有限公司 A kind of multispectral motion control method for fluorescence microscopy scope

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AV01 Patent right actively abandoned

Granted publication date: 20120829

Effective date of abandoning: 20140226

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