CN202401058U - Kit for detecting helicobacter pylori infection - Google Patents
Kit for detecting helicobacter pylori infection Download PDFInfo
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- CN202401058U CN202401058U CN2011203908072U CN201120390807U CN202401058U CN 202401058 U CN202401058 U CN 202401058U CN 2011203908072 U CN2011203908072 U CN 2011203908072U CN 201120390807 U CN201120390807 U CN 201120390807U CN 202401058 U CN202401058 U CN 202401058U
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Abstract
The utility model provides a kit for detecting helicobacter pylori infection, which comprises a kit body and a kit cover, wherein at least four independent compartments or grooves are arranged in the kit body. A first compartment or groove is used for containing carbamide, a second compartment or groove is used for containing sampling labels, a third compartment or groove is used for containing phenol red, a fourth compartment or groove is used for containing a detecting test tube, and a plurality of sampling labels arranged in the second compartment or groove are detachably fixed in the second compartment or groove and are packaged independently. The kit can detect the helicobacter pylori infection quickly, accurately and economically, and is suitable for clinic use.
Description
Technical field
The utility model belongs to field of biological detection, relates to a kind of test kit that is used for the rapid detection helicobacter pylori infection.
Background technology
Helicobacter pylori (Helicobacter pylori is that oral gets into one of gastral encountered pathogenic bacteria HP), its infection be chronic Type B gastritis main diseases because of, in close relations with non-ucler dyspepsia, gastric duodenal ulcer and cancer of the stomach.Epidemiological study shows, the HP infection rate is 39.9-49.6% in the asymptomatic or healthy population of compatriots.
For diagnosis, treatment and the prevention of chronic gastritis, peptide ulceration, the detection of helicobacter pylori has become clinical needs.Traditional helicobacter pylori detection method mainly comprises: 1) directly detect HP from stomach mucous membrane, comprise microbial culture, tissue smear and section statining; 2) the plain enzyme test of urea or
14C respiration test diagnosis HP infects; 3) immunological method like ELISA etc., has serology to detect again and method such as PCR in recent years.The standard that current diagnosis HP infects relates to pathology of gastric mucosa tissue examination and microbial culture, but these methods all take time and effort, and spend higherly, is unfavorable for clinical application, more is not suitable for mass detection, makes troubles to the detection of HP.
One of biological nature of helicobacter pylori is to be rich in the plain enzyme of urea; This also is one of its paathogenic factor; Therefore fast the plain enzymatic diagnosis method of urea has very high susceptibility and specificity, be widely used in clinical, but need detect through getting fresh biopsy stomach mucous membrane.
Dimension, how quick, accurate, economic mensuration HP infects is one of clinical problem that faces.
The utility model content
Therefore, the purpose of the utility model provides a kind of test kit that can the rapid detection helicobacter pylori infection.
For realizing above-mentioned purpose, the technical solution that the utility model provides is following:
A kind of test kit that detects helicobacter pylori infection; Said test kit comprises box body and lid; At least four independently compartment or grooves are set in the said box body; First compartment or groove are used to place urea, and second compartment or groove are used to place sampling pick, and the 3rd compartment or groove are used to place phenolsulfonphthalein; The 4th compartment or groove are used to place test tubes, and the sampling pick of placing in said second compartment or the groove is a plurality ofly to be fixed in this compartment or the groove and the sampling pick of independent packaging with removably.
The sampling pick of the independent packaging of preferably, placing in said second compartment or the groove is bonded in this compartment or the groove in proper order.
The test tubes of preferably, placing in said the 4th compartment or the groove is a plurality ofly to be fixed in the test tubes in this compartment or the groove with removably.
Preferably, said urea is the urea of the unit using dosage of a plurality of independent packagings.
Preferably, said first compartment or groove are divided into the unit compartment or the groove at a plurality of intervals, and each unit compartment or groove are used to the urea of the unit's of placement using dosage.
Preferably, said phenolsulfonphthalein is the phenolsulfonphthalein of the unit using dosage of a plurality of independent packagings.
Preferably, said the 3rd compartment or groove are divided into the unit compartment or the groove at a plurality of intervals, and each unit compartment or groove are used to the phenolsulfonphthalein of the unit's of placement using dosage.
Preferably, said test kit also comprises colorimetric, and said colorimetric independently is positioned in the said second or the 4th compartment or the groove, perhaps is fixed on the lid, perhaps is fixed on each test tubes of placing in said the 4th compartment or the groove.
The method of use of mentioned reagent box may further comprise the steps: 1) in test tubes, prepare detection reagent; 2) get fresh biopsy tongue fur sample with the sample label, place in the test tubes, hatched 1-10 minute; 3) observe the detection reagent colour-change.
Wherein, the compound method of detection reagent is following in the said step 1): in the phenolsulfonphthalein solution with the urea unit of the being added to using dosage of unit using dosage, add ultrapure water and be settled to 50ml.
In sum, the test kit of the quick qualitative detecting infection of helicobacter pylori that the utility model provides comprises box body, is located at the intravital helicobacter pylori detection reagent of box, helicobacter pylori detection indicator, test tubes, colorimetric, sampling pick.Preferably, comprise a bag urea in the test kit, one bottle of phenolsulfonphthalein, with many joint inspections testing tube of the test tubes and the colorimetric of the independent packaging of removably fixed, a bag sampling pick.The test tubes of desirable next independent packaging at any time detects during use, detects easily and fast, accurately.
Behind the plain enzyme serial dilution of 1% urea, when the plain enzyme minimum content of urea was 0.5mg/ml, the reaction solution color just changed redness into by yellow, and the plain enzyme content of urea is high more, and colour-change is faster and dark.In the test kit of above-mentioned quick qualitative detecting infection of helicobacter pylori,, run into the helicobacter pylori that can produce the plain enzyme of urea, thereby make indicator phenolsulfonphthalein color become light red to red-purple owing to contain urea in the detection reagent.Therefore, utilize this test kit, only, just can infect HP easily and carry out qualitative detection according to the colour-change of solution.
The test kit that the utility model provides has the following advantages and effect:
1) test kit is set to independently compartment or groove according to built-in various testing tools, and each testing tool is independent packaging, prevents the mutual pollution between the testing tool effectively, and test kit is stable, can preserve for a long time, and the quality guaranteed period is 12 months.
2) the various raw material sources of preparation test kit are abundant, cheap;
3) various testing tools, for example the independent packaging of detection reagent and the detection indicator unit of being using dosage is taken conveniently, and it is simple to detect step, only needs 1 people can accomplish the entire operation process;
4) detect and outside human body, to carry out, sample collection is convenient, avoids contacting of human body and chemical reagent, and is safe and reliable;
5) detection speed is fast, and one detection period is merely 1-10 minute, has reduced waste of manpower resource.
6) qualitative accurate, experiment showed, that accuracy rate is 98.4%.
Description of drawings
Fig. 1 is the internal structure synoptic diagram of the test kit that provides of the utility model;
Wherein 1 is first compartment or groove, and 2 is second compartment or groove, and 3 is the 3rd compartment or groove, and 4 is the 4th compartment or groove;
Fig. 2 is the result that the test kit that adopts the utility model to provide detects;
The negative detected result of A wherein, the positive detected result of B.
Embodiment
Below in conjunction with accompanying drawing and embodiment the utility model is described further.
Embodiment 1
As shown in Figure 1; Test kit is aligned in sequence with in order: first compartment or groove (are built-in with 100 independent packagings; Each independent packaging includes 2.75g urea), second compartment or groove (be built-in with 100 independent packagings, each independent packaging includes 2 sampling picks); (be built-in with 100 independent packagings, each independent packaging includes 0.09% phenolsulfonphthalein 5ml for the 3rd compartment or groove.First compartment or groove (be built-in with 100 independent packagings, each independent packaging includes 2 test tubes).The test kit second layer is the 4th compartment or groove (be built-in with 100 independent packagings, each independent packaging includes 2 test tubes).In addition, test kit also comprises the colorimetric that is directly fixed on the tray cover.
Embodiment 2
As shown in Figure 1; Be aligned in sequence with in order in the test kit: be aligned in sequence with in order in the test kit: first compartment or groove (are built-in with 50 independent packagings; Each independent packaging includes 2.75g urea), second compartment or groove (be built-in with 50 independent packagings, each independent packaging includes 2 sampling picks); The 3rd compartment or groove (are built-in with 50 independent packagings; Each independent packaging includes 0.09% phenolsulfonphthalein 5ml, the 4th compartment or groove (be built-in with 50 independent packagings, each independent packaging includes 2 test tubes).In addition, test kit also comprises the colorimetric that is directly fixed on said each test tubes.
Adopt test kit to detect helicobacter pylori infection, concrete operations are following:
1) in test kit first compartment or groove, gets the urea (2.75g) of a independent packaging;
2) in test kit the 3rd compartment or groove, get 0.09% phenolsulfonphthalein (5ml) of a independent packaging, wherein the compound method of 0.09% phenolsulfonphthalein is following: a) ratio in 0.9g/L takes by weighing some phenolsulfonphthaleins, carefully pours in the mortar; B) in the ratio of 2ml/L, in mortar, add 1mol/LNaOH solution, add 30～50ml zero(ppm) water more inward, grind 15～20min; C) pour in the beaker grinding uniform phenolsulfonphthalein, add the zero(ppm) water constant volume to aequum, the glass rod stirring and evenly mixing is placed about 10min.
3) in test kit the 4th compartment or groove, take out test tubes, in test tubes, add 5ml phenolsulfonphthalein solution, add 2.75g urea again, add ultrapure water at last and be settled to 50ml, be mixed with detection reagent;
4) in test kit second compartment or groove, take out the sample label, sign with sample and get fresh biopsy tongue fur sample, place in the test tubes;
5) room temperature is after 5 minutes, observations.
The detection reagent colour-change result who two parts of biopsy tongue fur samples is detected front and back is as shown in Figure 3, negative findings (no urase), and detection reagent (yellow)---keep yellow; Positive findings (urase is arranged), detection reagent (yellow)---become light red to red-purple.
Claims (8)
1. test kit that detects helicobacter pylori infection; Said test kit comprises box body and lid; It is characterized in that at least four independently compartment or grooves are set in the said box body, and first compartment or groove are used to place urea; Second compartment or groove are used to place sampling pick; The 3rd compartment or groove are used to place phenolsulfonphthalein, and the 4th compartment or groove are used to place test tubes, and the sampling pick of placing in said second compartment or the groove is a plurality ofly to be fixed in this compartment or the groove and the sampling pick of independent packaging with removably.
2. test kit according to claim 1 is characterized in that, the sampling pick of the independent packaging of placing in said second compartment or the groove is bonded in this compartment or the groove in proper order.
3. test kit according to claim 1 is characterized in that, the test tubes of placing in said the 4th compartment or the groove is a plurality ofly to be fixed in the test tubes in this compartment or the groove with removably.
4. test kit according to claim 1 is characterized in that, said urea is the urea of the unit using dosage of a plurality of independent packagings.
5. test kit according to claim 4 is characterized in that, said first compartment or groove are divided into the unit compartment or the groove at a plurality of intervals, and each unit compartment or groove are used to the urea of the unit's of placement using dosage.
6. test kit according to claim 1 is characterized in that, said phenolsulfonphthalein is the phenolsulfonphthalein of the unit using dosage of a plurality of independent packagings.
7. test kit according to claim 6 is characterized in that, said the 3rd compartment or groove are divided into the unit compartment or the groove at a plurality of intervals, and each unit compartment or groove are used to the phenolsulfonphthalein of the unit's of placement using dosage.
8. according to each described test kit in the claim 1 to 7; It is characterized in that; Said test kit also comprises colorimetric; Said colorimetric independently is positioned in the said second or the 4th compartment or the groove, perhaps is fixed on the lid, perhaps is fixed on each test tubes of placing in said the 4th compartment or the groove.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011203908072U CN202401058U (en) | 2011-10-14 | 2011-10-14 | Kit for detecting helicobacter pylori infection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011203908072U CN202401058U (en) | 2011-10-14 | 2011-10-14 | Kit for detecting helicobacter pylori infection |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202401058U true CN202401058U (en) | 2012-08-29 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011203908072U Expired - Lifetime CN202401058U (en) | 2011-10-14 | 2011-10-14 | Kit for detecting helicobacter pylori infection |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202401058U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103045468A (en) * | 2011-10-14 | 2013-04-17 | 深圳太太基因工程有限公司 | Kit used for detecting helicobacter pylori infection |
| CN105199948A (en) * | 2015-09-29 | 2015-12-30 | 窦晓鸣 | Quick detection kit and method of Escherichia coli |
-
2011
- 2011-10-14 CN CN2011203908072U patent/CN202401058U/en not_active Expired - Lifetime
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103045468A (en) * | 2011-10-14 | 2013-04-17 | 深圳太太基因工程有限公司 | Kit used for detecting helicobacter pylori infection |
| CN105199948A (en) * | 2015-09-29 | 2015-12-30 | 窦晓鸣 | Quick detection kit and method of Escherichia coli |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20120829 |