CN202256857U - Darkroom applicable to fluorescence microscopy imaging - Google Patents
Darkroom applicable to fluorescence microscopy imaging Download PDFInfo
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- CN202256857U CN202256857U CN2011202303706U CN201120230370U CN202256857U CN 202256857 U CN202256857 U CN 202256857U CN 2011202303706 U CN2011202303706 U CN 2011202303706U CN 201120230370 U CN201120230370 U CN 201120230370U CN 202256857 U CN202256857 U CN 202256857U
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- darkroom
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- microscopic imaging
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Abstract
The utility model relates to a darkroom applicable to fluorescence microscopy imaging. The darkroom comprises a combined support frame and a combined light shading cloth curtain, wherein the lower end of the combined support frame is fixedly arranged on an experiment platform, the combined support frame comprises at least three upright posts and at least three cross beams, each upright post is respectively connected with each cross beam, the connection modes comprise a bent angle connection mode and/or a T-shaped connection mode, the sun shading cloth curtain comprises a top cover cloth curtain, a side curtain and a front curtain and is covered and arranged above the combined support frame so that no light can be transmitted into the combined support frame, in addition, gaps allowing pipelines such as electric wires and flow filling pipelines to pass are arranged between the cloth curtains, and in addition, external light rays are ensured not to enter the combined support frame. Compared with the prior art, the darkroom and a manufacture method of the darkroom have the characteristics that the structure is simple, the manufacturing cost is low, the installation is convenient, the effect is ideal, the popularization is easy, and the like.
Description
Technical field
The utility model relates to a kind of darkroom, specifically, is a kind of darkroom that is applicable to fluorescent microscopic imaging.
Background technology
The utility model comes from the actual needs in the research practice.Along with improving constantly of scientific and technological level, micro-imaging technique obtains using extremely widely in biomedical research.Particularly, the appearance of fluorescent microscopic imaging technology (fluorescencemicroscopic imaging) has greatly promoted biomedical research and has made it to reach a new high.2008, U.S. scientist Qian Yongjian of Chinese origin was because of sharing Nobel chemistry Prize to the outstanding research of green fluorescent protein (Green Fluorescent Protein, be called for short GFP) with other scientists.GFP is the most frequently used a kind of luminescent protein in the fluorescent microscopic imaging research just.Up to the present, the microscope that is used for fluorescent microscopic imaging mainly contains common fluorescent microscope (epi-fluorescence microscope), total internal reflection fluorescent microscope (total internal reflection fluorescencemicroscope), confocal laser scanning microscope, CLSM (confocal laser scanning microscope) and two/multi-photon laser scanning microscope (two/multi-photon laser scanning microscope) etc.These microscopes respectively have its characteristics, are applicable to different research fields.But they have a common characteristic: all need in the half-light environment, work, reducing or to avoid photobleaching (photo-bleach) effect and the interference of ambient light signal to forming images of fluorescent dye, thereby obtain comparatively ideal imaging effect.
In the prior art, the common practice that adopt in all kinds of laboratories that utilize the fluorescent microscopic imaging technology to carry out biomedical research in the world wide is: in being placed on fluorescent microscopic imaging equipment and peripheral matched equipment thereof computer, earthquake table, laser and/or other light sources system and some other peripherals etc. of the software systems of control fluorescent microscopic imaging equipment (as be equipped with) between a dark place entirely; The experimenter carries out various experimental implementation between dark place.When needing illumination really, generally use dark room lamp.The experimenter works in the half-light environment and can bring a lot of unnecessary troubles, increases the maloperation probability; The beginner especially need make great efforts to overcome working environment and change the sense of discomfort brought and be inconvenient to feel.And the more important thing is: for high-sensitive fluorescent microscopic imaging, the light that light that computer screen sends and dark room lamp send all can cause bigger influence to imaging results.For removing this influence, experimental implementation person often gives gobo on the microscope body cover.This is effective to simple short time imaging research.But dynamic observe for a long time if desired, when particularly needing repeatedly experimental specimen to be applied medicine irritation or electro photoluminescence and writes down the variation of indexs such as its electrophysiology simultaneously, then can bring the inconvenience and the trouble that influences imaging effect of operation.And gobo is close to fuselage, and the time has been grown and also had the not smooth problem of heat radiation.And in fact, in the fluorescent microscopic imaging process, except that microscope host machine part (load has the biological sample of fluorescent dye just to be placed on its objective table part), other parts do not need the half-light environment; Experimenter's major part operation (the particularly relevant operation of computer Control Software) need not carried out under half-light yet.Therefore, do not receive the influence of light just can guarantee the effect of fluorescent microscopic imaging as long as guarantee the microscope host machine part.
The utility model content
The purpose of the utility model is to disclose a kind of ingenious, simple in structure, easy for installation, effective practicality, darkroom that is applicable to fluorescent microscopic imaging with low cost designed.
A kind of darkroom that is applicable to fluorescent microscopic imaging comprises the sectional shelf-unit and the combination shading door curtain made of cloth, and the sectional shelf-unit lower end is fixed on the experiment porch.It is characterized in that:
Described sectional shelf-unit comprises at least three root posts and at least three crossbeams, and each column is connected with each crossbeam respectively, and its connected mode comprises bent angle connected mode and/or T type connected mode;
The said shading door curtain made of cloth comprises the top cover door curtain made of cloth, limit curtain and preceding curtain; Covering is installed on the sectional shelf-unit; Make among the sectional shelf-unit light tightly, and between the door curtain made of cloth, be provided with the crack that electric wire and perfusion pipeline can pass through, and guarantee extraneous light not from then on crack get among the sectional shelf-unit.
Said bent angle connected mode is through an end of the two ends difference socket column of bent angle sleeve pipe and an end of crossbeam; T type connected mode connects the two sections of same root post and an end of crossbeam respectively through three ends of T molded cannula.
Said column or crossbeam can connect prolongation through straight molded cannula.
The position that contacts of the said all kinds of door curtains made of cloth overlaps each other and establishes VELCRO and makes its tight connection.
Said various sleeve pipe is to be threaded with an end of column or being connected of an end of crossbeam.
One end of said various sleeve pipe and column or an end of crossbeam are equipped with gland nut when being connected.
Described sectional shelf-unit can be by being threaded with the connected mode of experiment porch; Also can connect through sucker; Sucker base can be fixed in the experiment table top through powerful double faced adhesive tape; Said experiment porch is promptly put the shockproof platform (have plenty of the light face, have plenty of band standard screw) of fluorescent microscope.
Said column or crossbeam are generally the threaded metal bar.
The column of said sectional shelf-unit or crossbeam diameter are generally 6mm.
With existing technology relatively, that the utility model darkroom and manufacturing approach thereof have is simple in structure, cost of manufacture is low, easy for installation, satisfactory for result and be easy to characteristics such as popularization.This darkroom does not hinder and around camera lens, installs auxiliary equipment (patch-clamp register system of using always in like physiological and pharmacological research (patch clamping system) and administration perfusion system (perfusionsystem) etc.) on demand additional, and is compatible good; Thoroughly avoid the luminous and interference of darkroom light of computer screen, good shading effect to forming images; Institute's materials used is low value common material, cost, reduction of expenditure; From " dark ", free the user, improve working environment, increase work efficiency and quality; Dust cover also can be played in this darkroom.The dark place that generally uses in the present world wide intrinsic fluorescence of this small-sized omnipotent darkroom instead micro-imaging process.
Description of drawings
Below in conjunction with accompanying drawing the utility model is further detailed.
Fig. 1 is the structural representation of the utility model.
Fig. 2 is the combination rack structure synoptic diagram of the utility model, is example with the support of four root posts, and the column of practical application possibly be the six roots of sensation, eight even ten, decides according to actual needs; Can have three kinds to be connected between utility model column and the crossbeam: the T type connects, bent angle connects and the straight tube connected mode; The connection of T type is connected 360 degree regulated at will in the horizontal direction with bent angle; Thereby the horizontal cross sectional geometry that can guarantee sectional shelf-unit is an arbitrary polygon, confirms according to actual needs.Crossbeam between two root posts can be increased to two or three on demand.
Fig. 3 is the utility model column fixed form synoptic diagram: fastening with nut.
Fig. 4 is the fixing other type synoptic diagram of the utility model column: fix with powerful double faced adhesive tape and sucker.
Fig. 5 is that the utility model connects the synoptic diagram of going up the shading door curtain made of cloth.
Fig. 6 is that the utility model connects the synoptic diagram of going up the shading door curtain made of cloth and having outlet mechanism.
Wherein, 1. columns among the figure, 2. crossbeam, 3. the top cover door curtain made of cloth, 4. the limit curtain, 5. before curtain, 6. bent angle sleeve pipe, 7. gland nut, 8.T molded cannula 9. straight molded cannula, 10. column set nut, 11. suckers, 12. powerful double faced adhesive tapes, 13. cracks, 14. experiment porchs.
Embodiment
With reference to figure 1 and Fig. 4; The utility model is a kind of darkroom that is applicable to fluorescent microscopic imaging, and this darkroom mainly comprises three big ingredients, is combined by sectional shelf-unit and the combination shading door curtain made of cloth and experiment porch 14; Sectional shelf-unit is made up of column 1 and crossbeam 2; Comprise 2 at least three on 1 at least three on column and crossbeam, each root post 1 is connected with each root crossbeam 2 respectively, and its connected mode comprises bent angle connected mode and/or T type connected mode.And in practical application, the quantity of column 1 can be four, five, the six roots of sensation, eight, even ten, decide according to microscope in the utility model darkroom and the shared space of other optional equipment.Accordingly, the quantity of crossbeam 2 also is to be decided according to the actual requirements for any selection more than three or collocation arbitrarily.The combination shading door curtain made of cloth comprises the top cover door curtain made of cloth 3, limit curtain 4 and preceding curtain 5; Covering is installed on the sectional shelf-unit; Make among the sectional shelf-unit light tightly, and between the door curtain made of cloth, be provided with the crack 13 that pipelines such as electric wire and perfusion pipeline can pass through, and guarantee extraneous light not from then on crack get among the sectional shelf-unit.Referring to figs. 1 through Fig. 6, the bent angle connected mode is through an end of the two ends difference socket column of bent angle sleeve pipe and an end of crossbeam; T type connected mode connects the two sections of a root post and an end of crossbeam respectively through three ends of T molded cannula.Column 1 or crossbeam 2 can connect prolongation through straight molded cannula 9.The position that contacts of all kinds of door curtains made of cloth overlaps each other and establishes VELCRO and makes its tight connection.Various sleeve pipes are to be threaded with an end of column or being connected of an end of crossbeam.One end of various sleeve pipes and column or an end of crossbeam are equipped with gland nut when being connected.Sectional shelf-unit and the connected mode of experiment porch can be by being threaded, also can connecting through sucker, and sucker base can be fixed in the experiment table top through powerful double faced adhesive tape, and said experiment porch is promptly put the shockproof platform of fluorescent microscope.Column or crossbeam are the threaded metal bar, and optimum diameter is 6mm.With reference to Fig. 2 and Fig. 3, sectional shelf-unit can be to be connected by threaded Metallic rod and nut with the connected mode of experiment porch, also can connect through sucker.Sucker base can be fixed in the experiment table top through powerful double faced adhesive tape.Said experiment porch is promptly put the shockproof platform of fluorescent microscope, have plenty of the light face, has plenty of band standard screw.This manufacturing approach in the darkroom of fluorescent microscopic imaging that is applicable to comprises the following steps: (1) parameter measurement: the selected fluorescent microscopic imaging system that intends the small-sized omnipotent darkroom of assembling is (like FV1000MPE; The two-photon imaging system that Olympus is produced); Measure the design support various master datas required (like length and various special angle, column quantity definite etc.) with the door curtain made of cloth.(2) modelling: the Parameter Map in the satisfactory small-sized omnipotent darkroom of the various computer software of applying in a flexible way (like Office, Coreldraw, Photoshop etc.) design; (3) processing of support and installation: timbering material is wanted durable and is convenient to processing and installation, and inexpensive.What we advised using is the screw thread iron bar of diameter 6mm.By measured parameter, iron bar is cut into the iron bar section of appropriate length.And some with electric welding making T connector and angle coupling, other prepares nut and screwed pipe is some.Support divides column and two types of iron bars of crossbeam, is connected also fastening each other through accessories such as T connector, angle coupling, screwed pipe and nuts.For the standard vibration isolators that screw is arranged, column can directly screw in the screw of vibration isolators, and fastening with nut; If there is not the vibration isolators of screw, available sucker and powerful double faced adhesive tape vertical columns; (4) processing of the shading door curtain made of cloth and installation: the door curtain made of cloth will select the gobo of high-quality, to guarantee the lucifuge effect, considers price and processing problems simultaneously.The black door curtain made of cloth is paid the utmost attention in suggestion.The door curtain made of cloth divides three kinds on top cover (), limit curtain (more than) and preceding curtain ().Be installed on the support.The contact site has enough overlappingly during installation, and closely connects with VELCRO.That the utility model darkroom and manufacturing approach thereof have is simple in structure, cost of manufacture is low, easy for installation, satisfactory for result and be easy to characteristics such as popularization.This darkroom does not hinder and around camera lens, installs auxiliary equipment (patch-clamp register system of using always in like physiology and pharmaceutical research (patch clamping system) and administration perfusion system (perfusion system) etc.) on demand additional, and is compatible good; Thoroughly avoid the luminous and interference of darkroom light of computer screen, good shading effect to forming images; Institute's materials used is low value common material, cost, reduction of expenditure; From " dark ", free the user, improve working environment, increase work efficiency and quality; Dust cover also can be played in this darkroom.The dark place that generally uses in the present world wide intrinsic fluorescence of this small-sized omnipotent darkroom instead micro-imaging process.
Claims (9)
1. a darkroom that is applicable to fluorescent microscopic imaging comprises sectional shelf-unit and the combination shading door curtain made of cloth and experiment porch, it is characterized in that:
Described sectional shelf-unit comprises at least three root posts (1) and at least three crossbeams (2), and each root post (1) is connected with each root crossbeam (2) respectively, and its connected mode comprises bent angle connected mode and/or T type connected mode;
The said shading door curtain made of cloth comprises the top cover door curtain made of cloth (3), limit curtain (4) and preceding curtain (5); Covering is installed on the sectional shelf-unit; Make among the sectional shelf-unit light tightly, and between the door curtain made of cloth, be provided with the crack that electric wire and perfusion pipeline can pass through, and guarantee extraneous light not from then on crack get among the sectional shelf-unit.
2. the darkroom that is applicable to fluorescent microscopic imaging as claimed in claim 1 is characterized in that: said bent angle connected mode is through an end of the two ends difference socket columns (1) of bent angle sleeve pipe and an end of crossbeam (2); T type connected mode connects the two sections of a root post (1) and an end of crossbeam (2) respectively through three ends of T molded cannula.
3. the darkroom that is applicable to fluorescent microscopic imaging as claimed in claim 1 is characterized in that: said column (1) or crossbeam (2) can connect prolongation through straight molded cannula.
4. the darkroom that is applicable to fluorescent microscopic imaging as claimed in claim 1 is characterized in that: the position that contacts of the said all kinds of door curtains made of cloth overlaps each other and establishes VELCRO and makes its tight connection.
5. like claim 2 or the 3 described darkrooms that are applicable to fluorescent microscopic imaging, it is characterized in that: various sleeve pipes are to be threaded with an end of column (1) or being connected of an end of crossbeam (2).
6. like claim 2 or the 3 described darkrooms that are applicable to fluorescent microscopic imaging, it is characterized in that: an end of various sleeve pipes and column (1) or an end of crossbeam (2) are equipped with gland nut when being connected.
7. the darkroom that is applicable to fluorescent microscopic imaging as claimed in claim 1; It is characterized in that: described sectional shelf-unit can be by being threaded with the connected mode of experiment porch; Also can connect through sucker; Sucker base can be fixed in the experiment table top through powerful double faced adhesive tape, and said experiment porch is promptly put the shockproof platform of fluorescent microscope.
8. the darkroom that is applicable to fluorescent microscopic imaging as claimed in claim 1 is characterized in that: said column (1) or crossbeam (2) are the threaded metal bar.
9. the darkroom that is applicable to fluorescent microscopic imaging as claimed in claim 1 is characterized in that: column of said sectional shelf-unit (1) or crossbeam (2) diameter are 6mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011202303706U CN202256857U (en) | 2011-07-01 | 2011-07-01 | Darkroom applicable to fluorescence microscopy imaging |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011202303706U CN202256857U (en) | 2011-07-01 | 2011-07-01 | Darkroom applicable to fluorescence microscopy imaging |
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| Publication Number | Publication Date |
|---|---|
| CN202256857U true CN202256857U (en) | 2012-05-30 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011202303706U Expired - Fee Related CN202256857U (en) | 2011-07-01 | 2011-07-01 | Darkroom applicable to fluorescence microscopy imaging |
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| Country | Link |
|---|---|
| CN (1) | CN202256857U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110143251A (en) * | 2019-04-22 | 2019-08-20 | 台州市皓仔邦工业设计有限公司 | A kind of wind deflector of sunshade awning for electric car |
| CN110182283A (en) * | 2019-04-22 | 2019-08-30 | 台州市皓仔邦工业设计有限公司 | A kind of sunshade awning for electric car |
-
2011
- 2011-07-01 CN CN2011202303706U patent/CN202256857U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110143251A (en) * | 2019-04-22 | 2019-08-20 | 台州市皓仔邦工业设计有限公司 | A kind of wind deflector of sunshade awning for electric car |
| CN110182283A (en) * | 2019-04-22 | 2019-08-30 | 台州市皓仔邦工业设计有限公司 | A kind of sunshade awning for electric car |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120530 Termination date: 20130701 |