CN202175679U - Magneto-optic compound strain mutation instrument - Google Patents
Magneto-optic compound strain mutation instrument Download PDFInfo
- Publication number
- CN202175679U CN202175679U CN 201120312154 CN201120312154U CN202175679U CN 202175679 U CN202175679 U CN 202175679U CN 201120312154 CN201120312154 CN 201120312154 CN 201120312154 U CN201120312154 U CN 201120312154U CN 202175679 U CN202175679 U CN 202175679U
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- CN
- China
- Prior art keywords
- magneto
- casing
- mutagenesis
- transformer
- telefault
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/06—Magnetic means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Sustainable Development (AREA)
- Biomedical Technology (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Electromagnetism (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The utility model belongs to a strain mutation device and particularly relates to a magneto-optic compound strain mutation instrument, which comprises a box body, a box door packaged at the front part of the box body and a box cover arranged outside the box body. A control part consists of a power supply, an ultraviolet tube, a transformer, an inductance coil, a sliding rheostat and an indicating instrument, wherein the ultraviolet tube and a primary coil of the transformer are connected in parallel and respectively connected to two ends of the power supply; a secondary coil of the transformer and the inductance coil form a loop through the sliding rheostat; the indicating instrument is connected in the loop; the ultraviolet tube is arranged above the inside of the box body; and the transformer, the sliding rheostat, the inductance coil and the power supply are arranged in the box cover outside one side of the box body. The magneto-optic compound strain mutation instrument solves the problems of not easy realization of adjustment and control of the magnetic field intensity in the existing equipment and not obvious mutation effect, and has the advantages that the intensity can be adjusted, the strain mutation probability can be effectively improved by adopting the compound mutation, and the like.
Description
Technical field
The utility model belongs to the induction mutation of bacterium device, is meant a kind of magneto-optic composite bacteria mutagenesis appearance especially.
Background technology
The probability that transgenation takes place under the mikrobe standard state is 10
-6, the probability that forward mutation takes place is lower.Adopt single physical mutagenesis method little to the effect that microbial mutation produces, two or more the compound use of physical mutagenesis method is big to the effect that microbial mutation produces.The patent No. is to disclose a kind of mutagenesis inoculation dual-purpose case in the utility model patent of 03218655.x; This utility model realizes the mutagenesis of single one physical method to the bacterial classification of its inside through external magnetic stirring apparatus; Its intensity is difficult for realizing adjustment and control, and the mutagenesis DeGrain.
Summary of the invention
The purpose of the utility model is to provide a kind of magneto-optic composite bacteria mutagenesis appearance, through adopting uv illumination, and adds the adjustable magnetic field of intensity bacterial classification in the casing is carried out mutagenesis, reaches the purpose that improves mutation effect.
The overall technology design of the utility model is:
Magneto-optic composite bacteria mutagenesis appearance; Comprise casing 2, be packaged in casing 2 forward chamber doors 3, be arranged at the outside case lid 1 of casing 2; Control section comprises power supply, ultraviolet lamp tube D2, X-former T, telefault L, slide rheostat R and display instrument; Be connected to both ends of power respectively after ultraviolet lamp tube D2 and the transformer L1 parallel connection, transformer secondary output coil L2 constitutes the loop through slide rheostat R and telefault L, and display instrument is connected in the loop; Wherein ultraviolet lamp tube D2 is located at casing 2 inner and upper, and X-former T, slide rheostat R, telefault L, power supply are located in the outside case lid 1 of casing 2 one sides.
The concrete technical construction of the utility model also has:
For ease of carrying out the intravital induction mutation of bacterium operation of case; Observe the colonial morphology in the culture vessel in the operating process; Optimized technical scheme is: also establish a head light D1 in casing 2 inner and upper, be connected to both ends of power after this head light D1 and ultraviolet lamp tube D2, transformer L1 are parallelly connected.
For ease of the current in loop and the voltage condition at telefault L place are observed and controlled, optimized technical scheme is: display instrument comprises that series connection is arranged at the current in loop Table A, is connected in the voltmeter V at telefault L head and the tail two ends respectively; Voltmeter V and reometer A are located at case lid 1 surface.
Telefault L can select multiple existing forms such as comprising ferrite coil, iron-core coil for use according to practical situation, does not break away from the essence of the utility model.Wherein optimized technical scheme is to adopt iron-core coil.
For ease of slide rheostat R is controlled, with the magneticstrength that effective change telefault L is produced, the sliding end that preferred plan is slide rheostat R connects with the pilot lever that is positioned at case lid 1 surface 4.
The technical progress that the utility model is obtained is:
1, the utility model is incorporated into uv illumination and two kinds of physical mutagenesis methods of magnetic field mutagenesis on the induction mutation of bacterium appearance cleverly, and its structure design is ingenious, and is big to the induction mutation of bacterium probability.
2, because the adjustment of the exposure intensity of magneticstrength that telefault L is produced and uv lamp; All can realize controlling comparatively accurately; Thereby the origin cause of formation to induction mutation of bacterium that can science is analyzed; For scientific experiment is laid a good foundation, can effectively shorten the process of experiment simultaneously, help the suitability for industrialized production of induction mutation of bacterium.
Description of drawings
Fig. 1 is the structural representation of the utility model.
Fig. 2 is the schematic circuit of the utility model.
Fig. 3 is the rear view of Fig. 1.
Reference numeral in the utility model is following:
1, case lid, 2, casing, 3, chamber door, 4, pilot lever, A, reometer, D1, head light, D2, ultraviolet lamp tube, L, telefault, L1, primary winding, L2, secondary coil, R, slide rheostat, T, X-former, V, voltmeter.
Embodiment
Below in conjunction with accompanying drawing the embodiment of the utility model is done and to further describe; But not as qualification to the utility model; The protection domain of the utility model is as the criterion with the content of claim record, and any equivalence techniques means replacement of making according to this specification sheets does not all break away from the protection domain of the utility model.
The overall technology structure of present embodiment is as shown in the figure; Magneto-optic composite bacteria mutagenesis appearance wherein; Comprise casing 2, be packaged in casing 2 forward chamber doors 3, be arranged at casing 2 outside case lid 1, power supply, ultraviolet lamp tube D2, X-former T, telefault L, slide rheostat R and display instrument; Be connected to both ends of power respectively after ultraviolet lamp tube D2 and the transformer L1 parallel connection, transformer secondary output coil L2 constitutes the loop through slide rheostat R and telefault L, and display instrument is connected in the loop; Wherein ultraviolet lamp tube D2 is located at casing 2 inner and upper, and X-former T, slide rheostat R, telefault L, power supply are located in the outside case lid 1 of casing 2 one sides.Also establish a head light D1 in casing 2 inner and upper, be connected to both ends of power after this head light D1 and ultraviolet lamp tube D2, transformer L1 are parallelly connected.
Display instrument comprises that series connection is arranged at the current in loop Table A, and two ends are connected in the voltmeter V of telefault L head and the tail respectively; Voltmeter V and reometer A are located at case lid 1 surface.
Telefault L selects iron-core coil for use.
The sliding end of slide rheostat R connects with the pilot lever that is positioned at case lid 1 surface 4.
Power supply is selected the 220V AC power for use, and telefault L uses 0.5 millimeter copper conductor of diameter, twines the number of turn is 1000, and the wavelength of uv lamp is 260nm.
Through test, adopt in the present embodiment to sphingomonas paucimobilis (
Sphingomonas paucimobilis) carry out mutagenic treatment, treatment condition are that voltage is set to 50V with slide rheostat R " 0 " position, preheating 10 minutes is respectively with 10
-1, 10
-2, 10
-3, 10
-4Each 10ml of bacteria suspension puts into the petridish of magneto-optic mutagenesis appearance bottom half, and adjustment slide rheostat R resistance is 90 Ω, and this moment, electric current was 1A, mutagenic treatment 10 minutes.Dispose and close the mutagenesis appearance.
With the secondary fermentation that spreads cultivation of the bacterial classification after the above-mentioned mutagenesis, and compare with bacterial classification before the mutagenesis, it is 2.33% that the bacterial classification after the fermentation ends after the mutagenesis produces the glue ability, and it is 1.5% that the bacterial classification before the mutagenesis produces the glue ability.
Claims (5)
1. magneto-optic composite bacteria mutagenesis appearance comprises casing (2), is packaged in casing (2) forward chamber door (3), is arranged at the outside case lid (1) of casing (2); Control section comprises power supply, ultraviolet lamp tube (D2), X-former (T), telefault (L), slide rheostat (R) and display instrument; It is characterized in that being connected to both ends of power respectively after ultraviolet lamp tube (D2) and transformer (L1) parallel connection; Transformer secondary output coil (L2) constitutes the loop through slide rheostat (R) and telefault (L), and display instrument is connected in the loop; Wherein ultraviolet lamp tube (D2) is located at casing (2) inner and upper, and X-former (T), slide rheostat (R), telefault (L), power supply are located in the outside case lid (1) of casing (2) one sides.
2. magneto-optic composite bacteria mutagenesis appearance according to claim 1; It is characterized in that also establishing a head light (D1), be connected to both ends of power after this head light (D1) and ultraviolet lamp tube (D2), transformer (L1) are parallelly connected in casing (2) inner and upper.
3. magneto-optic composite bacteria mutagenesis appearance according to claim 1 is characterized in that display instrument comprises that series connection is arranged at current in loop table (A), is connected in the voltmeter (V) at telefault (L) head and the tail two ends respectively; Voltmeter (V) and reometer (A) are located at case lid (1) surface.
4. according to claim 1 or 3 described magneto-optic composite bacteria mutagenesis appearance, it is characterized in that telefault (L) selects iron-core coil for use.
5. magneto-optic composite bacteria mutagenesis appearance according to claim 1 is characterized in that the sliding end of slide rheostat (R) and the pilot lever (4) that is positioned at case lid (1) surface join even.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201120312154 CN202175679U (en) | 2011-08-24 | 2011-08-24 | Magneto-optic compound strain mutation instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201120312154 CN202175679U (en) | 2011-08-24 | 2011-08-24 | Magneto-optic compound strain mutation instrument |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202175679U true CN202175679U (en) | 2012-03-28 |
Family
ID=45865574
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201120312154 Expired - Lifetime CN202175679U (en) | 2011-08-24 | 2011-08-24 | Magneto-optic compound strain mutation instrument |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202175679U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113645776A (en) * | 2016-11-17 | 2021-11-12 | Lg伊诺特有限公司 | DC-DC converter |
-
2011
- 2011-08-24 CN CN 201120312154 patent/CN202175679U/en not_active Expired - Lifetime
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113645776A (en) * | 2016-11-17 | 2021-11-12 | Lg伊诺特有限公司 | DC-DC converter |
| CN113645776B (en) * | 2016-11-17 | 2024-02-13 | Lg伊诺特有限公司 | DC-DC converter |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| ASS | Succession or assignment of patent right |
Owner name: ZHANG YU Free format text: FORMER OWNER: ZHANG XINGHAO Effective date: 20130313 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20130313 Address after: 055650 Hebei Xinhe Biochemical Co., Ltd., No. 10, prosperous street, Chengxi Industrial Zone, New River County, Hebei, Xingtai Patentee after: Zhang Yu Address before: 055650, New River, Hebei province Xingtai County West Industrial Zone, 10 Hing Street, Hebei Xin He Biological Chemical Co., Ltd. Zhang Shaohua closed Patentee before: Zhang Xinghao |
|
| ASS | Succession or assignment of patent right |
Owner name: HEBEI HENGBIAO BIOTECHNOLOGY CO., LTD. Free format text: FORMER OWNER: ZHANG YU Effective date: 20130520 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20130520 Address after: 055650 No. 118, south side of west section of Xinhua Road, Hebei County, New River Province Patentee after: Hebei Henbo Bio-Technology Co., Ltd. Address before: 055650 Hebei Xinhe Biochemical Co., Ltd., No. 10, prosperous street, Chengxi Industrial Zone, New River County, Hebei, Xingtai Patentee before: Zhang Yu |
|
| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20120328 |