CN202158982U

CN202158982U - Biological sample storage device

Info

Publication number: CN202158982U
Application number: CN2011202360431U
Authority: CN
Inventors: 武利庆; 王晶; 杨彬; 毕佳明
Original assignee: National Institute of Metrology
Current assignee: National Institute of Metrology
Priority date: 2011-07-06
Filing date: 2011-07-06
Publication date: 2012-03-07
Anticipated expiration: 2021-07-06

Abstract

The utility model provides a biological sample storage device, which comprises a storage processing box, a temperature sensor, a single chip microcomputer, a relay circuit and a power supply. The storage processing box comprises a sample platform, a sample centrifugal tube, a peltier piece, a metal radiation fin and a radiation fan. One side of the peltier piece is bonded with the bottom surface of the sample platform, and the other side of the peltier piece is bonded with the upper surface with the metal radiation fin. The radiation fan is fixed under the metal radiation fin. The sample centrifugal tube and the temperature sensor are arranged in a hole groove of the sample platform. Temperature data collected by the temperature sensor are transmitted to the single chip microcomputer and compared by the single chip microcomputer. A connection or disconnection signal of the power supply of the peltier piece and a control signal changing the direction of current passing through the peltier piece are transmitted to the relay circuit connected with the peltier piece. The storage method of biological samples is provided. The biological sample storage device has the advantages of being high in temperature resolution, simple in structure, low in cost and strong in function expansion capability.

Description

The biological sample save set

Technical field

The utility model relates to biological technical field, particularly a kind of biological sample save set.

Background technology

Biological sample is often unstable, degrades easily when temperature is high, simultaneously with subsidiary reaction, so need carry out at 4 ℃ usually when sample retention and processing.But processes such as some treatment step such as enzymolysis, the temperature of reaction that need raise again is as carrying out so that keep the activity of enzyme at 37 ℃.Traditional biological sample is preserved the function that treatment facility does not possess the control cooling and heats up, and usually with manual completion, this is very inconvenient for carrying out biological chemistry and molecular biological research, and its control accuracy also is difficult to reach simultaneously.

So development not only can lower the temperature, but also can heat up, can be controlled at the biological sample save set that certain temperature keeps certain hour simultaneously, for carrying out biological chemistry and molecular biological research, problem is very important.

The utility model content

The purpose of the utility model is exactly the above-mentioned defective that will overcome prior art, provides a kind of temperature resolution high, and simple in structure, cost is low, the biological sample save set that function expansibility is strong.

For reaching above-mentioned purpose; The biological sample save set that the utility model provides comprises the preservation treatment box; Also comprise temperature sensor, single-chip microcomputer, relay circuit and power supply; Said preservation treatment box comprises sample stage, some sample centrifuge tubes, Pa Er card sheet, metal fin and radiator fan, and the one side of said Pa Er card sheet is sticked together through the bottom surface of heat conductive silica gel and said sample stage, and another side is also through heat conductive silica gel and saidly have the upper surface of metal fin to be sticked together; Said radiator fan is fixed on the below of said metal fin; Said sample stage has some hole slots, and said sample centrifuge tube places said hole slot, and said temperature sensor is installed in the said hole slot at middle part; The temperature data of said temperature sensor collection passes to said single-chip microcomputer; Said single-chip microcomputer is through the comparison of preset temperature data and collecting temperature data, and the make-and-break signal of said Pa Er card sheet power supply and the control signal that changes the direction of current through said Pa Er card sheet are transferred to said relay circuit, said relay circuit and electric linking to each other of said Pa Er card sheet.

The utility model biological sample save set, it is the unibus digital sensor of 18B20 that wherein said temperature sensor adopts model, directly with digital signaling output collecting temperature.

The utility model biological sample save set, it is the chip of AT89S52 that wherein said single-chip microcomputer adopts model, the circuit board of said AT89S52 chip has the DIP socket.

The utility model biological sample save set, wherein hole slot has two kinds of apertures, and said sample centrifuge tube adopts 1.5mL sample centrifuge tube or 2mL sample centrifuge tube.

The utility model biological sample save set, wherein said power supply comprise a 5V5A Switching Power Supply that is used for to single-chip microcomputer and radiator fan power supply, and another gives the 12V20A Switching Power Supply of Pa Er card sheet power supply.

The advantage of the utility model biological sample save set is: temperature sensor adopts the unibus digital sensor, and directly with digital signaling output, temperature resolution is high, can reach 0.0625 degree.Adopt Pa Er card sheet, single-chip microcomputer and relay circuit, simple in structure, cost is low, and function expansibility is strong.

Description of drawings

Fig. 1 is the front view of the utility model biological sample save set;

Fig. 2 is the side view of the utility model biological sample save set;

Fig. 3 is the vertical view of the utility model biological sample save set;

Fig. 4 is the block diagram of control system.

Embodiment

Specify the embodiment of a kind of biological sample save set of the utility model below in conjunction with accompanying drawing.

With reference to Fig. 1, to Fig. 4; The biological sample save set that the utility model provides comprises temperature sensor 5, preserves treatment box 7, single-chip microcomputer 8, relay circuit 9 and power supply; Wherein, preserve treatment box 7 and comprise sample stage 1, some sample centrifuge tube (not shown)s, Pa Er card sheet 2, metal fin 3 and radiator fan 4.

The one side of Pa Er card sheet 2 is sticked together through the bottom surface of heat conductive silica gel and sample stage 1, and another side is through heat conductive silica gel and have the upper surface of metal fin 3 to be sticked together.Radiator fan 5 is fixed on the below of metal fin 4.Sample stage 1 has some hole slots 6, and hole slot 6 has two kinds of apertures, and the sample centrifuge tube adopts 1.5mL sample centrifuge tube or 2mL sample centrifuge tube, places hole slot 6.

Temperature sensor 5 is installed in the hole slot 6 at middle part, and it is the unibus digital sensor of 18B20 that temperature sensor 5 adopts model, and directly with digital signaling output collecting temperature, member is simple, and temperature resolution can reach 0.0625 degree.

The temperature data that temperature sensor 5 is gathered passes to single-chip microcomputer 8; Single-chip microcomputer 8 is through the comparison of preset temperature data and collecting temperature data; The make-and-break signal of Pa Er card sheet 2 power supplies and the control signal that changes the direction of current through Pa Er card sheet 2 are transferred to relay circuit 9, and relay circuit 9 and Pa Er card sheet 2 electricity link to each other.

It is the chip of AT89S52 that single-chip microcomputer adopts model, and the circuit board of this AT89S52 chip has the DIP socket.

Power supply comprises that one is used for to the 5V5A Switching Power Supply 10 of single-chip microcomputer 8 with radiator fan 4 power supplies, and another gives the 12V20A Switching Power Supply 11 of Pa Er card sheet 2 power supplies.

In use; At first confirm the duration of sample preset temperature and each preset temperature; Then in the software of single-chip microcomputer 8 according to preset temperature and duration update routine, and the program after will compiling writes in the AT89S52 chip through programmable device, the AT89S52 chip inserted on the DIP socket of single-chip microcomputer 8; Open Switching Power Supply, device promptly gets into duty.Each common programs can write an AT89S52 chip.Distinct program directly unclamps the DIP socket when switching, and changes a slice chip then, and single-chip microcomputer 8 can be realized different functions.Certainly, also available with a slice single-chip microcomputer 8 repeat the programming.After getting into duty, the AT89S52 chip both can have been lowered the temperature and also can have been heated up, and by temperature programmed control, and can accomplish the function that different temperatures keeps different time.

Concrete temperature controlled processes is: when the temperature detection value of temperature sensor 5 in the sample stage 1 is higher than setting value; Single-chip microcomputer 8 control relay circuits 9; Give the power supply of Pa Er card sheet 2 forwards by 12V20A Switching Power Supply 11, the cold junction of Pa Er card sheet 2 links to each other with sample stage 1, realizes the cooling of sample stage 1.The hot junction of Pa Er card sheet 2 links to each other with fan, through fan cooled to guarantee that the obedient sheet 2 of Pa Er is not burnt.When sample stage 1 temperature reached the temperature of setting, single-chip microcomputer 8 control relay circuits 9 cut off the power supply of 12V20A Switching Power Supply 11, thereby realize the cooling temperature control of sample stage 1.

When the temperature detection value of temperature sensor 5 in the sample stage 1 is lower than setting value; Single-chip microcomputer 8 control relay circuits 9; Oppositely supply power for Pa Er card sheet 2 by 12V20A Switching Power Supply 11; The cold junction of Pa Er card sheet 2 links to each other with fan, and the hot junction of Pa Er card sheet 2 links to each other with sample stage 1, realizes the heating of sample stage 1.When sample stage 1 temperature reached the temperature of setting, single-chip microcomputer 8 control relay circuits 9 cut off the power supply of 12V20A Switching Power Supply 11, thereby realize the heating temperature control of sample stage 1.

The utility model biological sample save set, simple in structure, installation cost is very low, and single-chip microcomputer source program extendability is strong, can realize different functions according to user's needs flexible programming.

Embodiment recited above describes the preferred implementation of the utility model, is not that design and scope to the utility model limits.Under the prerequisite that does not break away from the utility model design concept; Various modification and improvement that this area ordinary person makes the technical scheme of the utility model; All should drop into the protection domain of the utility model; The technology contents that the utility model is asked for protection all is documented in claims.

Claims

1. biological sample save set; Comprise and preserve treatment box (7); It is characterized in that: also comprise temperature sensor (7), single-chip microcomputer (8), relay circuit (9) and power supply; Said preservation treatment box (7) comprises sample stage (1), some sample centrifuge tubes, Pa Er card sheet (2), metal fin (3) and radiator fan (4); The one side of said Pa Er card sheet (2) is sticked together through the bottom surface of heat conductive silica gel and said sample stage (1); Another side is through heat conductive silica gel and saidly have the upper surface of metal fin (3) to be sticked together, and said radiator fan (4) is fixed on the below of said metal fin (3), and said sample stage (1) has some hole slots (6); Said sample centrifuge tube places said hole slot (6); Said temperature sensor (5) is installed in the said hole slot (6) at middle part, and the temperature data that said temperature sensor (5) is gathered passes to said single-chip microcomputer (8), and said single-chip microcomputer (8) is through the comparison of preset temperature data and collecting temperature data; The make-and-break signal of said Pa Er card sheet (2) power supply is transferred to said relay circuit (9) with the control signal that changes through said Pa Er card sheet (2) direction of current, and said relay circuit (9) links to each other with said Pa Er card sheet (2) electricity.

2. biological sample save set according to claim 1 is characterized in that: it is the unibus digital sensor of 18B20 that wherein said temperature sensor (5) adopts model, directly with digital signaling output collecting temperature.

3. biological sample save set according to claim 2 is characterized in that: it is the chip of AT89S52 that wherein said single-chip microcomputer (8) adopts model, and the circuit board of said AT89S52 chip has the DIP socket.

4. biological sample save set according to claim 3 is characterized in that: wherein said hole slot (6) has two kinds of apertures, and said sample centrifuge tube adopts 1.5mL sample centrifuge tube or 2mL sample centrifuge tube.

5. biological sample save set according to claim 4 is characterized in that: wherein said power supply comprises a 5V5A Switching Power Supply (10) that is used for to single-chip microcomputer and radiator fan power supply, and another gives the 12V20A Switching Power Supply (11) of Pa Er card sheet power supply.