CN202022914U - Dot hybridization experimental device - Google Patents
Dot hybridization experimental device Download PDFInfo
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- CN202022914U CN202022914U CN2010206965566U CN201020696556U CN202022914U CN 202022914 U CN202022914 U CN 202022914U CN 2010206965566 U CN2010206965566 U CN 2010206965566U CN 201020696556 U CN201020696556 U CN 201020696556U CN 202022914 U CN202022914 U CN 202022914U
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- hybridization
- groove
- dot blot
- experimental installation
- blot experimental
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Abstract
The utility model discloses a dot hybridization experimental device. A hybridization groove of the dot hybridization experimental device is horizontal relative to a horizontal plane or is arranged obliquely in the length direction of the hybridization groove; when the hybridization groove is arranged horizontally, one end of the hybridization groove in the longitudinal direction is provided with a concave groove; and when the hybridization groove is arranged obliquely, one end of the hybridization groove which is lower than the horizontal plane is provided with the concave groove, blocking needles or a barrier for positioning a hybridization film is arranged between the hybridization film and the concave groove, so that the hybridization film is prevented from sliding to the concave groove. The bottom of the hybridization groove is flat, or one end of the hybridization groove facing the concave groove is oblique, so that liquid in the hybridization groove flows constantly into the concave groove during sucking of a test solution from the concave groove until all the liquid in the hybridization groove is sucked out through the concave groove, and the amount of residual test solution in the hybridization groove is extremely low. For preventing the hybridization film from sliding to the concave groove and influencing solution sucking, the blocking needles or the barrier is arranged between the hybridization film and the concave groove, so that liquid can flow, and the hybridization film can be positioned and is prevented from moving to the concave groove.
Description
Technical field
The utility model relates to a kind of biochemical test device, relates in particular to a kind of dot blot experimental installation.
Background technology
Dot blot requires whole Hybond membrane bar (nylon membrane bar) must be immersed in for a long time in the hybridization solution to obtain best hybridization environment.
The existing hybridization slot device that can replace carrying out being provided in the experiment of dot blot by hand has following two kinds:
1, uses the hybrid pipe (groove) of cross section as circular arc, this is present modal use-pattern, this method has following shortcoming: at first, the hybridization groove of circular arc needs more reaction reagent, and owing to need be immersed in the hybrid pipe of circular arc for a long time, find that curling or change song in various degree can appear in the edge of film bar so treat hybridization step end back, under the insufficient situation of the added hybridization liquid of next procedure, will cause that part of abnormal hybridization of curling, directly influence final judgement; The way that solves is with similar things such as paper clips the film bar to be fixed on container edge, and touches the sample area on the film bar, so inconvenience very; The most important thing is, when needs remove reaction reagent, can't blot the reagent under the Hybond membrane fully, need topple over.
2, common flat pan, because finishing whole hybrid experiment is to be carried out continuously by different steps, after a certain step end can appear in flat pan, it is not thorough to remove reaction solution, thereby cause debris too much, influence the effect of subsequent experimental, cause whole crossbreeding effect colour developing undesirable, so also need to topple over reaction solution.
Therefore, more than two kinds of cell bodies all cooperate switching mechanism to realize hybrid experiment, yet present switching mechanism stuck phenomenon in various degree can occur or overturn excessive, thereby cause the failure of an experiment, and the switching mechanism complex structure, cost is high, makes the complete machine cost too high.
The utility model content
The technical problem that the utility model mainly solves provides a kind of dot blot experimental installation that can remove reaction solution easily.
For solving the problems of the technologies described above, the technical scheme that the utility model adopts is: a kind of dot blot experimental installation is provided, the hybridization groove of described dot blot experimental installation is level or in the length direction setting that tilts of hybridization groove with respect to the horizontal plane, when the hybridization groove is horizontally disposed with, end on the hybridization slot length direction is provided with groove, when the hybridization groove is obliquely installed, the with respect to the horizontal plane lower end of hybridization groove is provided with groove, is provided with the retaining pin or the fence that are used to locate Hybond membrane between Hybond membrane and the groove.
Wherein, described dot blot experimental installation is provided with and drives the vibrating device that the hybridization groove periodically rocks in the longitudinal direction.
Wherein, described dot blot experimental installation is provided with heating constant-temperature equipment at the hybridization trench bottom.
Wherein, described dot blot experimental installation is provided with calutron below hybridization geosynclinal concave groove one end, and described hybridization geosynclinal concave groove one end is provided with and can be energized magnetic patch or the iron block that calutron attracts.
Wherein, be provided with the retaining pin of two settings between Hybond membrane and the groove.
Wherein, described hybridization groove is provided with hybridization groove lid.
Usefulness of the present utility model is, be different from existing dot blot experimental installation, the hybridization groove of dot blot experimental installation of the present utility model has been done significant improvement, end at the hybridization trench bottom is provided with groove, because the hybridization trench bottom is smooth or to the inclination of groove one end, when therefore drawing test solution in groove, the liquid in the hybridization groove will constantly flow in the groove, whole liquid all is sucked out by groove in the hybridization groove, thereby makes the test solution residual quantity in the hybridization groove extremely low.And, influencing imbibition for avoiding Hybond membrane to slide into groove, the utility model also is provided with retaining pin or fence between Hybond membrane and groove, not only liquid flow is crossed but also can be located Hybond membrane, makes it can not move to groove.
Description of drawings
Fig. 1 is the hybridization groove vertical view of the described dot blot experimental installation of the utility model embodiment;
Fig. 2 is the hybridization groove front section view of the described dot blot experimental installation of the utility model embodiment;
Fig. 3 is the hybridization groove side sectional view of the described dot blot experimental installation of the utility model embodiment;
Fig. 4 is the described dot blot experimental installation of a utility model embodiment partial schematic diagram one;
Fig. 5 is the described dot blot experimental installation of a utility model embodiment partial schematic diagram two;
Fig. 6 is the described dot blot experimental installation of a specific embodiment of the invention schematic perspective view.
Label declaration:
1, hybridization groove 11, groove 12, retaining pin 2, hybridization groove lid 3, spring bearing 4, calutron 5, eccentric straight-line running gear 51, motor 52 motors are equipped with eccentric wheel, 53 traces 54, snib 6, linear slide 8, thermostatically heating plate
Embodiment
By describing technology contents of the present utility model, structural attitude in detail, realized purpose and effect, give explanation below in conjunction with embodiment and conjunction with figs. are detailed.
See also Fig. 1 to Fig. 5, present embodiment provides a kind of dot blot experimental installation, the hybridization groove 1 of described dot blot experimental installation is level or in the length direction setting that tilts of hybridization groove with respect to the horizontal plane, when the hybridization groove is horizontally disposed with, end on the hybridization slot length direction is provided with groove 11, when the hybridization groove is obliquely installed, the with respect to the horizontal plane lower end of hybridization groove is provided with groove 11, be provided with the retaining pin 12 or the fence that are used to locate Hybond membrane between Hybond membrane and the groove, make Hybond membrane can not slide into groove 11 places.
During experiment, undertaken by following steps:
Step 1: place the hybridization groove to carry out hybridization the Hybond membrane bar with reagent, described hybridization groove is level or in the length direction setting that tilts of hybridization groove with respect to the horizontal plane, when the hybridization groove is horizontally disposed with, end on the hybridization slot length direction is provided with groove, when the hybridization groove is obliquely installed, the with respect to the horizontal plane lower end of hybridization groove is provided with groove, is provided with the retaining pin or the fence that are used to locate Hybond membrane between Hybond membrane and the groove, makes Hybond membrane can not slide into groove;
Step 2: when needing to change liquid in the hybridization, will will hybridize liquid sucking-off in the groove, and put into novel agent in the hybridization groove then and continue hybridization by groove;
Step 3: hybridization finishes, and will hybridize liquid sucking-off in the groove by groove.
The hybridization groove of dot blot experimental installation of the present utility model has been done significant improvement, end at the hybridization trench bottom is provided with groove, because the hybridization trench bottom is smooth or tilts to groove one end, when therefore in groove, drawing test solution, liquid in the hybridization groove will constantly flow in the groove, whole liquid all is sucked out by groove in the hybridization groove, thereby makes the test solution residual quantity in the hybridization groove extremely low.And, influencing imbibition for avoiding Hybond membrane to slide into groove, the utility model also is provided with retaining pin or fence between Hybond membrane and groove, not only liquid flow is crossed but also can be located Hybond membrane, makes it can not move to groove.
Referring to Fig. 5,6, as the improvement of the foregoing description, described dot blot experimental installation is provided with vibrating device, and described vibrating device drives the hybridization groove and periodically rocks in the longitudinal direction.
In the present embodiment, described vibrating device is specific as follows: the side below hybridization groove 1 is provided with a snib structure, be provided with a motor 51 in a corresponding side, motor is provided with eccentric wheel 52, trace 53 1 ends connect snib 54, one end connects eccentric wheel 52, has constituted eccentric straight-line running gear 5 thus.When motor 51 drove eccentric wheel 52 rotations, the linear slide 6 that slide block below eccentric wheel 52, connecting rod 53, snib 54 and base plate and guide rail are formed can drive hybridization grooves 1 front and back and rock back and forth.(purpose of rocking: will be in the process of hybridization the liquid that splash into easier, scatter faster, it is contacted with the Hybond membrane bar fully, the process of hybridization is carried out fully.) label 2 is described is hybridization groove lid 2, be used for avoiding dust etc. to fall in the hybridization groove 1 and and outwards spill at the vibration processes reaction solution.
Referring to Fig. 4,5, as the improvement of first embodiment, described dot blot experimental installation is provided with heating constant-temperature equipment, and described heating constant-temperature equipment heats the hybridization groove, and keeps the temperature of setting.Because need a constant temperature heating device in crossover process, so present embodiment is provided with a thermostatically heating plate 8 below the hybridization cell body, an end of hot-plate is provided with power connection.
Referring to Fig. 4, Fig. 5 improvement as above-mentioned 3 embodiment, described dot blot experimental installation is provided with calutron 4 below hybridization geosynclinal concave groove one end, described hybridization geosynclinal concave groove one end is provided with and can be energized magnetic patch or the iron block that calutron 4 attracts, when calutron 4 energisings, hybridization groove 1 tilts to an end at calutron place.
Specific as follows: the hybridization cell body is fixed on the support by the spring bearing 3 that is arranged on four jiaos of hybridization cell bodies, and support is fixed on the base plate with bolt, is provided with a slide block and is installed on the guide rail below base plate.Between a end that the hybridization groove 1 of hybridization cell body has a groove 11 and plate upper surface, be connected with calutron 4.When needing the hybridization solution sucking-off after one step of hybridization, at this time give calutron 4 energisings, energising back calutron produces magnetic force will hybridize cell body toward drop-down, make in the groove 11 of the easier inflow hybridization of hybridization solution groove.But during the calutron no power, the elastic force of spring bearing 3 will be hybridized groove and be set back and put.
Referring to Fig. 1 to Fig. 3,, be provided with the retaining pin 12 of two settings between Hybond membrane and the groove 11 as the improvement of embodiment 1 to 3.
The above only is embodiment of the present utility model; be not so limit claim of the present utility model; every equivalent structure transformation that utilizes the utility model specification sheets and accompanying drawing content to be done; or directly or indirectly be used in other relevant technical fields, all in like manner be included in the scope of patent protection of the present utility model.
Claims (6)
1. dot blot experimental installation, it is characterized in that, the hybridization groove of described dot blot experimental installation is level or in the length direction setting that tilts of hybridization groove with respect to the horizontal plane, when the hybridization groove is horizontally disposed with, end on the hybridization slot length direction is provided with groove, when the hybridization groove was obliquely installed, the with respect to the horizontal plane lower end of hybridization groove was provided with groove, is provided with the retaining pin or the fence that are used to locate Hybond membrane between Hybond membrane and the groove.
2. dot blot experimental installation according to claim 1 is characterized in that, described dot blot experimental installation is provided with and drives the vibrating device that the hybridization groove periodically rocks in the longitudinal direction.
3. dot blot experimental installation according to claim 1 is characterized in that, described dot blot experimental installation is provided with heating constant-temperature equipment at the hybridization trench bottom.
4. according to any described dot blot experimental installation of claim 1 to 3, it is characterized in that, described dot blot experimental installation is provided with calutron below hybridization geosynclinal concave groove one end, described hybridization geosynclinal concave groove one end is provided with and can be energized magnetic patch or the iron block that calutron attracts.
5. according to any described dot blot experimental installation of claim 1 to 3, it is characterized in that, be provided with the retaining pin of two settings between Hybond membrane and the groove.
6. according to any described dot blot experimental installation of claim 1 to 3, it is characterized in that described hybridization groove is provided with hybridization groove lid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010206965566U CN202022914U (en) | 2010-12-31 | 2010-12-31 | Dot hybridization experimental device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010206965566U CN202022914U (en) | 2010-12-31 | 2010-12-31 | Dot hybridization experimental device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202022914U true CN202022914U (en) | 2011-11-02 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010206965566U Expired - Lifetime CN202022914U (en) | 2010-12-31 | 2010-12-31 | Dot hybridization experimental device |
Country Status (1)
| Country | Link |
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| CN (1) | CN202022914U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102559835A (en) * | 2010-12-31 | 2012-07-11 | 福建泰普生物科学有限公司 | Dot hybridization method and dot hybridization experimental device |
| CN104152342A (en) * | 2014-07-09 | 2014-11-19 | 湖北省农业科学院畜牧兽医研究所 | Constant-pressure transmembrane clamp for hybridization of Southern imprinting |
-
2010
- 2010-12-31 CN CN2010206965566U patent/CN202022914U/en not_active Expired - Lifetime
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102559835A (en) * | 2010-12-31 | 2012-07-11 | 福建泰普生物科学有限公司 | Dot hybridization method and dot hybridization experimental device |
| CN102559835B (en) * | 2010-12-31 | 2014-01-08 | 福建泰普生物科学有限公司 | Dot hybridization method and dot hybridization experimental device |
| CN104152342A (en) * | 2014-07-09 | 2014-11-19 | 湖北省农业科学院畜牧兽医研究所 | Constant-pressure transmembrane clamp for hybridization of Southern imprinting |
| CN104152342B (en) * | 2014-07-09 | 2016-08-17 | 湖北省农业科学院畜牧兽医研究所 | Southern marking hybridization constant voltage transferring film is pressed from both sides |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C56 | Change in the name or address of the patentee |
Owner name: TRIPLEX INTERNAT BIOSCIENCES (CHINA) CO., LTD. Free format text: FORMER NAME: FUJIAN TRIPLEX INTERNATIONAL BIOSCIENCES CO., LTD. |
|
| CP01 | Change in the name or title of a patent holder |
Address after: 350002, No. 7, No. two, No. 7, Jinzhou North Road, Jinshan Industrial Zone, Fujian, Fuzhou, four floors Patentee after: Triplex International Biosciences (China) Co., Ltd. Address before: 350002, No. 7, No. two, No. 7, Jinzhou North Road, Jinshan Industrial Zone, Fujian, Fuzhou, four floors Patentee before: Fujian Triplex International Biosciences Co., Ltd. |
|
| AV01 | Patent right actively abandoned |
Granted publication date: 20111102 Effective date of abandoning: 20140108 |
|
| RGAV | Abandon patent right to avoid regrant |