CN202002933U - Syphilis specificity total-antibody and cardiolipin antibody immunoblotting kit - Google Patents
Syphilis specificity total-antibody and cardiolipin antibody immunoblotting kit Download PDFInfo
- Publication number
- CN202002933U CN202002933U CN 201120025696 CN201120025696U CN202002933U CN 202002933 U CN202002933 U CN 202002933U CN 201120025696 CN201120025696 CN 201120025696 CN 201120025696 U CN201120025696 U CN 201120025696U CN 202002933 U CN202002933 U CN 202002933U
- Authority
- CN
- China
- Prior art keywords
- antibody
- syphilis
- cuorin
- syphilis specific
- kit
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Abstract
A syphilis specificity total-antibody and cardiolipin antibody immunoblotting kit relates to a kit. The kit is provided with a carrier plate, a nitrocellulose membrane, a syphilis specificity total-antibody and cardiolipin antibody detecting line and a control line. The detecting line and the control line are arranged on the nitrocellulose membrane, syphilis specificity recombinant antigen and cardiolipin antigen are coated at the positions of total-antibody and the detecting line, human Ig (immunoglobulin) antibody is coated at the position of the control line, and horse radish peroxidase marks the human Ig antibody. The syphilis specificity recombinant antigen includes TPN (triphospho-pyridine-nucleotide) 15, TPN17, TPN37, TPN44.5, TPN47 and the like. The syphilis specificity total-antibody and cardiolipin antibody immunoblotting kit can be used for detecting syphilis specificity total-antibody and cardiolipin antibody in a specimen such as whole blood, blood serum, blood plasma, cerebrospinal fluid and the like. The quantity of the required specimen is extremely small, special instruments are omitted, and further the syphilis specificity total-antibody and cardiolipin antibody immunoblotting kit is simple, convenient and fast in detection and high in specificity and flexibility, and is accurate and reliable.
Description
Technical field
The utility model relates to a kind of kit, especially relates to a kind of syphilis specific total antibodies and cuorin antibody mediated immunity trace kit.
Background technology
Syphilis (Syphilis) is that a kind of (its pathogen is a microspironema pallidum for Treponema pallidum, the sexually transmitted disease that TP) causes, belongs to Spirochaetaceae by microspironema pallidum.Approach such as the main trafficability characteristic contact of microspironema pallidum, blood transfusion, wound or placenta are propagated.Microspironema pallidum enters blood near the lymph node the infected area, sends out whole body, and nearly all tissue of body and organ are got involved, and clinical manifestation is a general, can be divided into different clinical stages, comprises first phase, second phase, three phases and latent period.The World Health Organization (WHO) is prophesy optimistically once: " because high sensitivity detection method and therapeutic scheme are efficiently arranged, syphilis is a kind of sexually transmitted disease that can succeed and control by the public health measure ".Regrettably, so far syphilis still is worldwide public health problem, lack effective administrative control measure, annual nearly 1,200 ten thousand the patient in the whole world, 600,000 pregnant woman patients ([1] Lin Lirong, Yang Bo, Pan Xitao wherein, Deng. the selection of patient's syphilis serology detection method is propagated in potential blood source. Chinese hospital infection magazine .2010,20 (10): 1491-1494).In fact, the infection present situation of syphilis is possibly than more allowing people's pessimism in the imagination.
Investigation finds that syphilis the person be present among the general population more widely.
Microspironema pallidum still can not carry out in vitro culture, and controller used in syphilis diagnosis and epidemiology survey mainly depend on serological test, comprises that specific antibody and reagin detect two major types.Syphilis specific antibody IgM (TP-IgM) and IgG (TP-IgG) antibody are respectively at 2 weeks and the back generation of 4 weeks, even the patient is through enough treatments, it still can long-term existence, even ([2] Lin Lirong that do not disappear all the life, but ice, Fu Zuogen, etc. patient syphilis serology TRUST/TPPA and the antibody combined detection of IgM are propagated in potential blood source. Chinese skin cypridology magazine .2010,152 (05): 446-448); And that another kind of antibody materials reagin produces is later, generally produce in 5～7 weeks of infected back, and late syphilis, syphilis treatment later stage and latent syphilis may be negative, so the positive rate of syphilis specific antibody, susceptibility is significantly higher than reagin.After TP-IgM is syphilis, the specific antibody that body occurs at first.As long as there is microspironema pallidum alive to exist, its TP-IgM will maintain certain level.([3] MartinaH such as Martina H, Daan W N, Mart M, et al.Comparison of a Treponema pallidum IgM immunoblot with a 19Sfluorescent treponemal antibody absorption test for the diagnosis of congenital syphilis[J] Diagnostic Microbiology and Infectious Disease, 2007,59:61-66.) think that TP-IgM is a syphilis early infection and a movable serologic marker, ([4] Li Burong such as Li Burong, He Juntao, Zhang Yi, Deng. the clinical meaning [J] of microspironema pallidum IgM antibody test. The Fourth Military Medical University's journal, 2007,28 (16): 1495-1497) think that TP-IgM is the same with TP-DNA, representing syphilis infectiousness index.Under the prerequisite of the recent anti-syphilis treatment of eliminating, TP-IgM is not if turn out cloudy, and remaining microspironema pallidum of possibility or treatment are not thorough in the prompting body.The cloudy commentaries on classics person of TP-IgM changes positive when following up a case by regular visits to again, show once more syphilization ([5] Rawstron SA, Mehta S, Bromberg K, et al.Evaluation of a Treponema pallidum2 specific IgM enzyme immunoassay and Treponema pallidum western blot antibody detection in the diagn
Early stage serological method uses complete microspironema pallidum as antigen, the TP of research and diagnosis usefulness obtains with TP infected rabbits testis, this method exists that cost is big, the TP amount that obtains less and impure (being mixed with host protein), have shortcomings such as cross reaction with other pathogen, so false positive also happens occasionally.Along with the clone in succession who reaches treponemal antigen that popularizes of Protocols in Molecular Biology, it is more and more that recombinant antigen is applied to the syphilis experiment.The many TP antigen of research has TPN17, TPN47, TPN15, TPN44.5, TPN36, TP0453, TP0684 and TPr family at present.The recombinant antigen that adopts recombinant DNA technology to prepare can overcome the shortcoming of complete TP antigen, can prepare endless special reorganization TP antigen fast, economically.
The syphilis specific antibody detection method comprises microspironema pallidum specific antibody GAT (TPPA), enzyme linked immunosorbent assay (ELISA), FTA-ABS test (FTA-ABS) and immunoblot assay (Western-Blot) etc., and its specificity is all higher.Usually, TPPA, ELISA be as clinical primary dcreening operation, and when the serology positive, or clinical diagnosis needs to adopt FTA-ABS and Western-blot as validation test when having any problem.Wherein, FTA-ABS needs fluorescent microscope, and its result judges and need do some training very often and just can finish than the professional of rich experiences that therefore, its application is subjected to certain restriction.The kit that adopts the Western-blot method to make, general Routine Test Lab all can be finished, and does not need specific installation, and interpretation is also simple and clear.Existing commercially available Western-blot reagent is import reagent, costs an arm and a leg.
Summary of the invention
The purpose of this utility model provides a kind of syphilis specific total antibodies and cuorin antibody mediated immunity trace kit.
The utility model is provided with carrier board, nitrocellulose membrane, syphilis specific total antibodies and cuorin antibody detection line, control line, and syphilis specific total antibodies and cuorin antibody detection line and control line are located on the nitrocellulose membrane successively; Wrap by syphilis specific recombinant antigen and cuorin antigen at syphilis specific total antibodies and cuorin antibody detection line place, wrap at the control line place by people Ig antibody; The anti-people Ig of horseradish peroxidase-labeled antibody.
Described syphilis specific recombinant antigen can be TPN15, TPN17, TPN37, TPN44.5, TPN47 etc.
Described carrier board can adopt the PVC plate.
The utility model can adopt following method preparation:
1) preparation syphilis specific recombinant antigen
Adopt gene clone technology, the DNA of pcr amplification coding treponemal antigen, and make its expression in the insertion Escherichia coli, get the syphilis specific recombinant antigen.
2) preparation cuorin antigen
The pig myocardium tissue is air-dry behind low-temperature quick-freezing, grinds to form behind the powdery with methyl alcohol and chloroform extracting, and extract is by the silica gel column chromatography wash-out, the cuorin antigen of dna purity more than 95%, and described methyl alcohol and chloroform, methyl alcohol by volume: chloroform can be 2: 1.
3) point sample of nitrocellulose filter
Bag by people Ig antibody, is dried at control line place bag by syphilis specific recombinant antigen and cuorin antigen on the total antibody detection line of nitrocellulose filter.
4) the total specific fragment Ig of the anti-people of preparation chain monoclonal antibody
With the total specific fragment Ig of people is antigen immune Balb/c mouse, and by hybridoma technology, screening obtains the hybridoma cell strain of the anti-people Ig of stably excreting monoclonal antibody, adopts the method identification of M cAb of ELISA to tire at 1: 10
7More than.
5) horseradish peroxidase (HRP) mark of anti-people Ig monoclonal antibody
Adopt the sodium periodate method to carry out horseradish peroxidase (HRP) mark, the concentration of described syphilis specific recombinant antigen and the total antibody of people can be 1～4mg/mL, and the point sample amount can be 1 μ L/cm; The concentration of described cuorin antigen can be 0.003%～0.03%, and the point sample amount can be 1 μ L/cm.
6) preparation HP immunoblotting kit
Nitrocellulose membrane is sticked on the carrier board upper surface, and syphilis specific total antibodies and cuorin antibody detection line and control line are located on the nitrocellulose membrane successively; Wrap by syphilis specific recombinant antigen and cuorin antigen at syphilis specific total antibodies and cuorin antibody detection line place, wrap by people Ig antibody at the control line place, be cut into strip with cutting cutter, dress up syphilis specific total antibodies and cuorin antibody mediated immunity trace kit with the anti-people Ig antibody and the substrate mutual group thereof of enzyme labeling, the substrate of described horseradish peroxidase can be made up of the diaminobenzidine of the hydrogen peroxide and 0.07% (W/V) of 0.03% (W/V).
In step 1), 3), 6) in, described syphilis specific recombinant antigen is TPN15, TPN17, TPN37, TPN44.5, TPN47 etc.
The utility model provides a kind of employing immunoblot assay to set up syphilis specific total antibodies and cardiolipin antibody detection kit, can be used for syphilis specific total antibodies and cuorin detection of antibodies in the samples such as whole blood, serum, blood plasma and cerebrospinal fluid.The required specimen amount of this detection method is minimum, does not need specific apparatus, the direct sentence read result of naked eyes, and detect easy fast, high specificity, highly sensitive, accurately and reliably, cost is low, is widely used.
Description of drawings
Fig. 1 is that the structure of the utility model embodiment is formed synoptic diagram.
Fig. 2 is the experimental result pattern diagram.In Fig. 2, J is the synoptic diagram before using, and I is invalid test (product quality problem), the negative result of H, and A～F is a TP-specific total antibodies positive findings; G is the anticardiolipin antibody positive findings.2 is the total antibody detection line of syphilis specific TPN-15-, 3 is the total antibody detection line of syphilis specific TPN-17-, 4 is the total antibody detection line of syphilis specific TPN-37-, 5 is the total antibody detection line of syphilis specific TPN-44.5-, 6 is the total antibody detection line of syphilis specific TPN-47-, 7 is the cuorin antibody detection line, and 8 is control line.
Embodiment
Following examples will be further described the utility model in conjunction with the accompanying drawings.
Referring to Fig. 1, the utility model embodiment is provided with carrier board 1, syphilis specific total antibodies and cuorin antibody detection line 2～7, control line 8 and nitrocellulose membrane (NC film) 9.
Nitrocellulose membrane 9 sticks on carrier board 1 upper surface, and syphilis specific total antibodies and cuorin antibody detection line 2～7 and control line 8 are located on the nitrocellulose membrane 9 successively; Wrap respectively by syphilis specific recombinant antigen TPN15, TPN17, TPN37, TPN44.5, TPN47 and cuorin antigen at syphilis specific total antibodies and cuorin antibody detection line place, wrap by people Ig antibody at control line 8 places.
Described carrier board adopts the PVC plate.
The preparation method of described syphilis specific total antibodies and cuorin antibody mediated immunity trace kit may further comprise the steps:
1) preparation TPN15, TPN17, TPN37, TPN44.5, TPN47 syphilis specific recombinant antigen
Adopt gene clone technology, the DNA of pcr amplification coding treponemal antigen, and make its expression in the insertion Escherichia coli, get TPN15, TPN17, TPN37, TPN44.5, TPN47 syphilis specific recombinant antigen.
2) preparation cuorin antigen
The pig myocardium tissue is air-dry behind low-temperature quick-freezing, grinds to form behind the powdery with methyl alcohol and chloroform extracting, and extract is by silica gel column chromatography wash-out, the cuorin antigen of dna purity more than 95%.
3) point sample of nitrocellulose filter
Bag by people Ig antibody, is dried at control line place bag by TPN15, TPN17, TPN37, TPN44.5, TPN47 syphilis specific recombinant antigen and cuorin antigen on the total antibody detection line of nitrocellulose filter.
4) the total specific fragment Ig of the anti-people of preparation chain monoclonal antibody
With the total specific fragment Ig of people is antigen immune Balb/c mouse, and by hybridoma technology, screening obtains the hybridoma cell strain of the anti-people Ig of stably excreting monoclonal antibody.Adopt the method identification of M cAb of ELISA to tire at 1: 10
7More than.
5) horseradish peroxidase (HRP) mark of anti-people Ig monoclonal antibody
Adopt the sodium periodate method to carry out horseradish peroxidase (HRP) mark.
6) preparation HP immunoblotting kit
Nitrocellulose membrane is sticked on the carrier board upper surface, and syphilis specific total antibodies and cuorin antibody detection line and control line are located on the nitrocellulose membrane successively; Wrap by TPN15, TPN17, TPN37, TPN44.5, TPN47 syphilis specific recombinant antigen and cuorin antigen at syphilis specific total antibodies and cuorin antibody detection line place, wrap by people Ig antibody at the control line place, the anti-people Ig antibody and the substrate mutual group thereof that are cut into strip and enzyme labeling with cutting cutter are dressed up syphilis specific total antibodies and cuorin antibody mediated immunity trace kit.
Below provide the clinical samples that adopts syphilis specific total antibodies and cuorin antibody mediated immunity trace kit to detect the patient:
1) sample adopts 0.01mol/L PBS damping fluid to carry out dilution in 1: 50.
2) sealing: adopt 5% skimmed milk power (preparation of 0.01mol/LPBST damping fluid) as confining liquid, waving incubation 30min on the shaking table in room temperature (18～25 ℃).
3) serum incubation: take out required film bar, put it in the incubation groove, and numbering.In the incubation groove, add 1.5ml diluted sample respectively.Waving incubation 30min on the shaking table in room temperature (18～25 ℃).
4) clean: inhale and remove liquid in the groove, waving on the shaking table with 1.5ml 0.01mol/L PBS buffer solution for cleaning film bar 3 times each 5min.
5) add enzyme conjugates: in the incubation groove, add the enzyme conjugates (the anti-people Ig monoclonal antibody that adopts horseradish peroxidase-labeled is for detecting antibody) that 1.5ml has diluted, waving incubation 30min on the shaking table in room temperature (18～25 ℃).
6) clean: inhale and remove liquid in the groove, waving on the shaking table with 1.5ml 0.01mol/L PBS buffer solution for cleaning film bar 3 times each 5min.
7) substrate incubation: in the incubation groove, add 1.5ml substrate solution (DAB) respectively, waving incubation 10min on the shaking table in room temperature (18～25 ℃).
8) stop: inhale and remove liquid in the groove, clean the film bar 3 times, each 1min with distilled water.
The result judges: will detect the film bar and be placed on the result and judge in the template air-dry judged result afterwards.Any protein targets marks existing specific band, all can be judged as the positive, is diagnosed as the syphilis (see figure 2).
Below provide the performance calibrating of syphilis specific total antibodies and cuorin antibody mediated immunity trace kit:
1) visual examination: kit does not have breakage, and bag is by smooth, the clean pollution-free spot of reaction film, free from flaw, and adhesive tape does not have and comes unglued, and does not have and cuts oblique phenomenon.
2) positive sample coincidence rate: the positive control serum with the different titers of the total antibody of TP-, the TRUST positive adopts syphilis specific total antibodies and the calibrating of cuorin antibody mediated immunity trace kit for each 50 parts, calculates positive coincidence rate.Definite employing FTA-ABS of positive control serum (German Ou Meng company) method and the definite clinical samples of TRUST (Xiamen Ying Kexin wound).
3) negative sample coincidence rate:, calculate positive coincidence rate with 50 parts of negative control serum calibratings.Definite employing TPPA (Japanese fuji Co., Ltd.) of negative control serum and the clinical samples that TRUST (Xiamen Ying Kexin wound) method is determined.
4) criticize in difference: same batch of syphilis specific total antibodies and cuorin antibody mediated immunity trace kit, detect with characteristic serum, require the positive serum testing result to show the shade unanimity of colour band, the feminine gender as a result that negative serum detects.
5) differences between batches: different batches syphilis specific total antibodies and cuorin antibody mediated immunity trace kit, detect with characteristic serum, require the shade unanimity of positive serum testing result demonstration colour band, the feminine gender as a result that negative serum detects.
6) interference test: testing result is not subjected to the interference of sample hemolysis (n=50), piarhemia (n=50) and jaundice (n=50).
7) cross reaction: adopt this syphilis specific total antibodies and cuorin antibody mediated immunity trace kit, carry out the detection of systemic loupus erythematosus (n=30), rheumatoid disease (n=30), autoallergic autoimmunity systemic diseases such as (n=30), do not find cross reaction.
8) Detection of Stability: use the Arrhenius rule, syphilis specific total antibodies and cuorin antibody mediated immunity trace kit are placed 37 ℃ to be detected after 20 days, more than every index do not have marked change, guarantee that finished product preserves under the drying at room temperature condition, the term of validity is 18 months.
Below provide specific embodiment.
Embodiment 1
Nitrocellulose membrane is sticked on the carrier board upper surface, and syphilis specific total antibodies and cuorin antibody detection line and control line are located on the nitrocellulose membrane successively; Wrap by TPN15, TPN17, TPN37, TPN44.5, TPN47 syphilis specific total antibodies and cuorin antigen at syphilis specific total antibodies and cuorin antibody detection line place, wrap by people Ig antibody at the control line place, the anti-people Ig and the substrate mutual group thereof that are cut into strip and enzyme labeling with cutting cutter are dressed up syphilis specific total antibodies and cuorin antibody mediated immunity trace kit.
1) sample adopts 0.01mol/L PBS damping fluid to carry out dilution in 1: 50.
2) sealing: adopt 5% skimmed milk power (preparation of 0.01mol/LPBST damping fluid) as confining liquid, waving incubation 30min on the shaking table in room temperature (18～25 ℃).
3) serum incubation: take out required film bar, put it in the incubation groove, and numbering.In the incubation groove, add 1.5ml diluted sample respectively.Waving incubation 30min on the shaking table in room temperature (18～25 ℃).
4) clean: inhale and remove liquid in the groove, waving on the shaking table with 1.5ml 0.01mol/L PBS buffer solution for cleaning film bar 3 times each 5min.
5) add enzyme conjugates: in the incubation groove, add the enzyme conjugates (the anti-people Ig monoclonal antibody that adopts horseradish peroxidase-labeled is for detecting antibody) that 1.5ml has diluted, waving incubation 30min on the shaking table in room temperature (18～25 ℃).
6) clean: inhale and remove liquid in the groove, waving on the shaking table with 1.5ml 0.01mol/L PBS buffer solution for cleaning film bar 3 times each 5min.
7) substrate incubation: in the incubation groove, add 1.5ml substrate solution (DAB) respectively, waving incubation 10min on the shaking table in room temperature (18～25 ℃).
8) stop: inhale and remove liquid in the groove, clean the film bar 3 times, each 1min with distilled water.
The result judges: will detect the film bar and be placed on the result and judge in the template air-dry judged result afterwards.Any protein targets marks existing specific band, all can be judged as the positive, is diagnosed as the syphilis (see figure 2).
Similar to embodiment 1, its difference is that nitrocellulose membrane only is made up of TPN15, TPN17, TPN37, TPN44.5 syphilis specific total antibodies and cuorin antigen, does not contain the TPN47 syphilis specific total antibodies.The result judges identical with embodiment 1.
Similar to embodiment 1, its difference is that nitrocellulose membrane only is made up of TPN15, TPN17, TPN37, TPN47 syphilis specific total antibodies and cuorin antigen, does not contain the TPN44.5 syphilis specific total antibodies.The result judges identical with embodiment 1.
Similar to embodiment 1, its difference is that nitrocellulose membrane only is made up of TPN15, TPN17, TPN44.5, TPN47 syphilis specific total antibodies and cuorin antigen, does not contain the TPN37 syphilis specific total antibodies.The result judges identical with embodiment 1.
Embodiment 5
Similar to embodiment 1, its difference is that nitrocellulose membrane only is made up of TPN15, TPN37, TPN44.5, TPN47 syphilis specific total antibodies and cuorin antigen, does not contain the TPN17 syphilis specific total antibodies.The result judges identical with embodiment 1.
Similar to embodiment 1, its difference is that nitrocellulose membrane only is made up of TPN17, TPN37, TPN44.5, TPN47 syphilis specific total antibodies and cuorin antigen, does not contain the TPN15 syphilis specific total antibodies.The result judges identical with embodiment 1.
Similar to embodiment 1, its difference is that sample to be checked is a samples of CSF, and the result judges identical with embodiment 1.
The performance verification test: the scheme by embodiment 1 prepares syphilis specific total antibodies and cuorin antibody mediated immunity trace kit, carries out performance verification then.
1) visual examination: kit does not have breakage, and bag is by smooth, the clean pollution-free spot of reaction film, free from flaw, and adhesive tape does not have and comes unglued, and does not have and cuts oblique phenomenon.
2) positive sample coincidence rate: the positive control serum with the different titers of the total antibody positive of TP-and the TRUST positive adopts syphilis specific total antibodies and the calibrating of cuorin antibody mediated immunity trace kit for each 50 parts, calculates positive coincidence rate.Definite employing FTA-ABS of positive control serum (German Ou Meng company) method and the definite clinical samples of TRUST (Xiamen Ying Kexin wound).
3) negative sample coincidence rate:, calculate positive coincidence rate with 50 parts of negative control serum calibratings.The clinical samples that definite employing TPPA (Japanese fuji Co., Ltd.) method of negative control serum is determined.
4) criticize in difference: same batch of syphilis specific total antibodies and cuorin antibody mediated immunity trace kit, detect with characteristic serum, require the positive serum testing result to show the shade unanimity of colour band, the feminine gender as a result that negative serum detects.
5) differences between batches: different batches syphilis specific total antibodies and cuorin antibody mediated immunity trace kit, detect with characteristic serum, require the shade unanimity of positive serum testing result demonstration colour band, the feminine gender as a result that negative serum detects.
6) interference test: testing result is not subjected to the interference of sample hemolysis (n=50), piarhemia (n=50) and jaundice (n=50).
7) cross reaction: adopt this syphilis specific total antibodies and cuorin antibody mediated immunity trace kit, carry out the detection of systemic loupus erythematosus (n=30), rheumatoid disease (n=30), autoallergic autoimmunity systemic diseases such as (n=30), do not find cross reaction.
8) Detection of Stability: use the Arrhenius rule, syphilis specific total antibodies and cuorin antibody mediated immunity trace kit are placed 37 ℃ to be detected after 20 days, more than every index do not have marked change, guarantee that finished product preserves under the drying at room temperature condition, the term of validity is 18 months.
Claims (3)
1. syphilis specific total antibodies and cuorin antibody mediated immunity trace kit, it is characterized in that being provided with carrier board, nitrocellulose membrane, syphilis specific total antibodies and cuorin antibody detection line, control line, syphilis specific total antibodies and cuorin antibody detection line and control line are located on the nitrocellulose membrane successively; Wrap by syphilis specific recombinant antigen and cuorin antigen at syphilis specific total antibodies and cuorin antibody detection line place, wrap at the control line place by people Ig antibody; The anti-people Ig of horseradish peroxidase-labeled antibody.
2. syphilis specific total antibodies as claimed in claim 1 and cuorin antibody mediated immunity trace kit is characterized in that described syphilis specific recombinant antigen is TPN15, TPN17, TPN37, TPN44.5, TPN47 syphilis specific recombinant antigen.
3. syphilis specific total antibodies as claimed in claim 1 and cuorin antibody mediated immunity trace kit is characterized in that described carrier board adopts the PVC plate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201120025696 CN202002933U (en) | 2011-01-25 | 2011-01-25 | Syphilis specificity total-antibody and cardiolipin antibody immunoblotting kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201120025696 CN202002933U (en) | 2011-01-25 | 2011-01-25 | Syphilis specificity total-antibody and cardiolipin antibody immunoblotting kit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202002933U true CN202002933U (en) | 2011-10-05 |
Family
ID=44705670
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201120025696 Expired - Fee Related CN202002933U (en) | 2011-01-25 | 2011-01-25 | Syphilis specificity total-antibody and cardiolipin antibody immunoblotting kit |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202002933U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102095861A (en) * | 2011-01-25 | 2011-06-15 | 厦门大学附属中山医院 | Western blot kit for syphilis specific total antibodies and anticardiolipin antibodies and preparation method of kit |
-
2011
- 2011-01-25 CN CN 201120025696 patent/CN202002933U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102095861A (en) * | 2011-01-25 | 2011-06-15 | 厦门大学附属中山医院 | Western blot kit for syphilis specific total antibodies and anticardiolipin antibodies and preparation method of kit |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN111366728A (en) | Immunochromatography kit for detecting novel coronavirus SARS-CoV-2 | |
| CN101738473B (en) | Treponema pallidum antibody diagnostic kit and preparation method thereof | |
| CN101858914B (en) | Reagent strip for testing syphilis specific total antibodies through gold immunochromatographic assay and preparation method thereof | |
| CN101825634A (en) | Reagent strip for joint detection of syphilis specific IgM and IgG antibodies and preparation method thereof | |
| CN101825636B (en) | Reagent strip for joint detection of syphilis specific IgM antibody and specific total antibody and preparation method thereof | |
| CN101858916A (en) | Reagent strip for testing syphilis specific IgM antibodies through gold immunochromatographic assay and preparation method thereof | |
| CN103091500A (en) | Antibody detection kit (OmpC-IgA) | |
| CN101825635B (en) | Reagent strip for joint detection of syphilis specific IgG antibody and specific total antibody and preparation method thereof | |
| CN101858915A (en) | Reagent strip for testing syphilis specific IgG antibodies through gold immunochromatographic assay and preparation method thereof | |
| CN201673156U (en) | Colloidal gold immunochromatographic test reagent strip for syphilis specific IgM antibody | |
| CN102095858A (en) | Western blot kit for cardiolipin and specific IgM antibodies of syphilis and preparation thereof | |
| CN103983792B (en) | A kind of immunoglobulin (Ig) lateral chromatography detection system | |
| CN201926664U (en) | Kit for immunoblot assay of specific IgG antibody against Treponema pallidum | |
| CN201955343U (en) | Syphilis cardiolipin and specific antibody IgM western-blot kit | |
| CN201926662U (en) | Syphilis specificity total antibody immunoblotting kit box | |
| CN201965132U (en) | Syphilis-specific IgM antibody immune-blotting kit | |
| CN102095860B (en) | Syphilis specific IgG antibody western blot kit and preparation method thereof | |
| CN102368068B (en) | Kit for detecting chlamydia pneumoniae IgM antibody | |
| CN202002933U (en) | Syphilis specificity total-antibody and cardiolipin antibody immunoblotting kit | |
| CN201926663U (en) | Immunoblotting kit for cardiolipin antibodies and syphilis specific immunoglobulin G (IgG) antibodies | |
| CN102081096A (en) | Syphilis cardiolipin and specific antibody IgG (Immunoglobulin G) immunoblotting kit and preparation method thereof | |
| CN101738474A (en) | Combined test reagent card for cytomegalovirus and rubella virus | |
| Inouye et al. | Oligomeric immunoglobulin A antibody response to rubella virus infection | |
| CN204028084U (en) | People's Chlamydia pneumoniae quantum dot immune chromatography test card | |
| CN201673158U (en) | Syphilis specificity IgG antibody collaurum immunity chromatographic detection test strip |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20111005 Termination date: 20140125 |