CN201952432U - Osteogenic seeding cell integrated dynamic inoculation culture matched bioreactor - Google Patents
Osteogenic seeding cell integrated dynamic inoculation culture matched bioreactor Download PDFInfo
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- CN201952432U CN201952432U CN 201120009776 CN201120009776U CN201952432U CN 201952432 U CN201952432 U CN 201952432U CN 201120009776 CN201120009776 CN 201120009776 CN 201120009776 U CN201120009776 U CN 201120009776U CN 201952432 U CN201952432 U CN 201952432U
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- transmodulator
- peristaltic pump
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/10—Perfusion
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Abstract
The utility model discloses an osteogenic seeding cell integrated dynamic inoculation culture matched bioreactor, which consists of a peristaltic pump, a perfusion chamber, a liquid storage bottle, a connecting pipe, and first, second, third and fourth channel converters, wherein the peristaltic pump is connected with one end of the perfusion chamber through the third channel converter, and the other end of the perfusion chamber is divided into two paths through the first channel converter; one path is communicated with the waterproof peristaltic pump through the second channel converter, and the other path is connected with the liquid storage bottle; and the liquid storage bottle is connected with the second channel converter through the fourth channel converter. During use, switching between two loops can be carried out by regulating the channel converters, so that the effects of integrated dynamic inoculation and dynamic culture are achieved; and a conversion link is avoided, a more suitable environment is provided for cell adhesion, growth, distribution, differentiation and tissue construction, and the effect of constructing tissue engineering bones is obviously enhanced.
Description
Technical field
The utility model belongs to biological technical field, relate to a kind of bio-reactor, be particularly related to the supporting bio-reactor of the integrated dynamic inoculation culture of a kind of skeletonization seed cell, can significantly improve support and carry intravital mass transfer efficient and scope, increase cell inoculation efficient and culture effect.
Background technology
At present, bone is damaged is the FAQs of orthopaedics in clinical.But in therapeutic process,, need the row second operation because of reasons such as soft tissue embedding, unsuitable internal fixing, shortage blood fortune, osteoporosis, metabolic disease, infection cause fracture delayed union or nonunion.Bone grafting is that the treatment bone is damaged and promote one of important means of union of fracture, adopt the autologous bone transplanting repairing bone defect because of its can provide for the growth of scleroblast and osseous tissue skeletonization precursor cell in support (osteoconductive), the inducing peripheral tissue in defect area divide a word with a hyphen at the end of a line and to osteoblast differentiation (osteoinductive), directly provide the skeletonization activity that can participate in, form the scleroblast characteristics such as (osteogenesis) of new bone, be still " gold standard " of bone transplanting so far.But, limited from body bone bone amount, get osseous surgery increased the patient wound and painful, prolonged operating time, and exist for the district infect, complication such as hemorrhage, pain.
The damaged clinical treatment of bone that develops into of the proposition of organizational project notion and bone tissue engineer technology provides a kind of new bone graft: tissue engineered bone.Utilize tissue engineering technique at the external structure osseous tissue, mainly be that the skeletonization seed cell is compound on the various timbering materials, the effect of uniting factors such as physics, chemistry and biotic factor again promotes cell growth, propagation and skeletonization differentiation, the process of final formative tissue.Therefore, it has osteoconductive, osteoinductive and osteogenesis concurrently, is " ideal bone graft ".Simultaneously, tissue engineered bone is not subjected to the bone quantitative limitation, does not have for district's complication and immunological rejection, bacterium and virus disease transmission danger.
The static inoculation of cell is to use the earliest and the most general engineering tissue external structure method with cultivating.Method is simple for this, and many researchs also use static method external structure tissue engineered bone also to obtain success in the experiment of dystopy skeletonization or bone defect repair in animal body.But along with the raising that Tissue Engineering Study turns to clinical application gradually and aspects such as organizational project bone volume, form and bone formation performance are required, static inoculation and cultural method self inherent defective reveal gradually.The cell of static inoculation relies on gravity, negative pressure or capillary action to enter support usually, is difficult to it is controlled, cause cell inoculation efficient low and in support skewness.Nutrition supply that the cell growth of static cultivation is required and gaseous interchange are that dispersion is realized by the concentration gradient difference of material, this makes nutritive substance and oxygen all very limited to the transmission efficiency and the scope of internal stent, cause internal stent nutrition and oxygen supply deficiency, cause cell only can " hollowization " of tissue occur making up in the survival of support periphery.In addition, cell inoculation and cell cultures are commonly two separate links, need artificial timbering material with inoculating cell to be transferred in the culture vessel, and this has obviously increased the danger of microbial contamination, has also influenced the tissue construction effect.
Summary of the invention
At defective or deficiency that above-mentioned prior art exists, the purpose of this utility model is, provides a kind of skeletonization seed cell integrated dynamic inoculation culture mating perfusion formula bio-reactor.
In order to realize above-mentioned task, the utility model is taked following technical solution:
The supporting bio-reactor of the integrated dynamic inoculation culture of a kind of skeletonization seed cell is characterized in that, is made of by pipe connecting and first, second, third, fourth channel converter peristaltic pump, perfusate chamber and reservoir; Wherein, peristaltic pump links to each other by third channel transmodulator one end with perfusate chamber, and the other end of perfusate chamber is divided into two-way by the first channel transmodulator, leads up to second passage transmodulator and waterproof type peristaltic pump to be communicated with, and another road connects reservoir; Reservoir also is connected with the second passage transmodulator by the four-way transmodulator.
Other characteristics of the present utility model are:
Described peristaltic pump is communicated with by third channel transmodulator and perfusate chamber, and perfusate chamber is communicated with by first channel transmodulator and reservoir, and reservoir is communicated with peristaltic pump to constitute by second passage transmodulator and four-way transmodulator cultivates loop;
Described peristaltic pump is communicated with by third channel transmodulator and perfusate chamber, and perfusate chamber is communicated with peristaltic pump to constitute by first channel transmodulator and second passage transmodulator inoculates loop.
Described peristaltic pump is the waterproof type peristaltic pump.
Described pipe connecting is the ventilation property pipe.
Adopt the supporting bio-reactor of the integrated dynamic inoculation culture of skeletonization seed cell of the present utility model, make up in the essential cell inoculation and culturing process at tissue engineered bone, carry out the integrated dynamic inoculation and the cultivation of cell by filling type bioreactor, strengthen cell inoculation efficient and uniformity coefficient, increase nutrition and oxygen supply scope and efficient, provide shearing stress to stimulate to scleroblast simultaneously, make cell inoculation and cultivate integrated carrying out in same stable environment, avoided conversion links, existing cell inoculation and culture technique are cell adhesion, growth, distribute, differentiation and tissue construction provide environment preferably, have significantly strengthened the effect that tissue engineered bone makes up.
Description of drawings
Fig. 1 is related filling type bioreactor structure and a working state schematic representation thereof of the utility model; Connecting pipeline among the figure is represented with mark 9, thick arrow mark 10 expression perfusion culture states, thin arrow mark 11 expression perfusion inoculation states.
The utility model is described in further detail below in conjunction with accompanying drawing.
Embodiment
With reference to Fig. 1, the supporting bio-reactor of the integrated dynamic inoculation culture of skeletonization seed cell of the present utility model, by waterproof type peristaltic pump 1, perfusate chamber 2, reservoir 3, first, second, third and four-way transmodulator (4,5,6,7) and pipe connecting 9 form the double loop structure of perfusion inoculation loops 10 and perfusion culture loop 11.Wherein, peristaltic pump 1 links to each other by third channel transmodulator 6 one ends with perfusate chamber 2, and the other end of perfusate chamber 2 is divided into two-way by first channel transmodulator 4, and second passage transmodulator 5 and the waterproof type peristaltic pump 1 of leading up to is communicated with, and another road connects reservoir 3; Reservoir 3 also is connected with second passage transmodulator 5 by four-way transmodulator 7.In the use, can between two loops, switch by regulating first channel transmodulator 4 and second passage transmodulator 5:
Peristaltic pump 1 is communicated with by third channel transmodulator 6 and perfusate chamber 2, perfusate chamber 2 is communicated with by first channel transmodulator 4 and reservoir 3, and reservoir 3 is communicated with waterproof type peristaltic pump 1 to constitute by second passage transmodulator 5 and four-way transmodulator 7 cultivates circulation loop 11;
Peristaltic pump 1 is communicated with by third channel transmodulator 6 and perfusate chamber 2, and perfusate chamber 2 is communicated with waterproof type peristaltic pump 1 to constitute by first channel transmodulator 4 and second passage transmodulator 5 inoculates circulation loop 10;
Peristaltic pump 1 is the waterproof type peristaltic pump, provide perfusion power by the waterproof type peristaltic pump, placing rack material 8 in the perfusate chamber 2, store nutrient solution in the reservoir 3, be connected the third channel transmodulator 6 that perfusate chamber 2 goes on the pipe and be used to the cell suspension of annotating, be connected reservoir 3 and go out that four-way transmodulator 7 on the pipe is used to change nutrient solution and sampling detects.Pipe connecting 9 in the loop is the ventilation property pipe, can keep the inside and outside gaseous interchange freely of bio-reactor.
Adopt the supporting bio-reactor of the integrated dynamic inoculation culture of skeletonization seed cell of the present utility model, when being used for the integrated dynamic inoculation culture of skeletonization seed cell that tissue engineered bone makes up, choose corresponding perfusate chamber according to the difference of timbering material, timbering material 8 is pre-loaded into the perfusate chamber 2 back whole loops of connection and makes it airtight fully, behind the filling type bioreactor overall disinfection first channel transmodulator 4 and second passage transmodulator 5 are adjusted to perfusion inoculation state (loops of thick arrow 10 expressions among the figure), close perfusion culture loop 11 and in inoculation circulation loop 10, fill with nutrient solution through third channel transmodulator 6.To inoculate circulation loop and be installed on the peristaltic pump 1, place 5%CO
2, change fresh medium behind the 12h in 37 ℃ of incubators, in loop, inject cell suspension 5mL by third channel transmodulator 6, open peristaltic pump 1, pour into inoculation to set speed.
Close inoculation circulation loop 10 after the perfusion inoculation finishes, in reservoir 3, add nutrient solution, open perfusion culture loop 11, carry out perfusion culture to set speed, compound up to cell in the timbering material internal breeding, obtain the cell-scaffold complex body.
The cell-scaffold complex body that obtains is implanted in animal or human's body studies or treats.
In cell inoculation and culturing process that tissue engineered bone makes up, adopt the supporting bio-reactor of the integrated dynamic inoculation culture of this skeletonization seed cell to carry out the integrated dynamic inoculation of cell and dynamically cultivation, strengthen cell inoculation efficient and uniformity coefficient, increase nutrition and oxygen supply scope and efficient, provide shearing stress to stimulate to scleroblast simultaneously, make cell inoculation and cultivate integrated carrying out in same stable environment, avoided conversion links, existing cell inoculation and culture technique are cell adhesion, growth, distribute, differentiation and tissue construction provide environment preferably, have significantly strengthened the effect that tissue engineered bone makes up.
Claims (4)
1. supporting bio-reactor of the integrated dynamic inoculation culture of skeletonization seed cell, it is characterized in that, constitute by pipe connecting (9) and first, second, third four-way transmodulator (4,5,6,7) by peristaltic pump (1), perfusate chamber (2) and reservoir (3); Wherein, peristaltic pump (1) links to each other by third channel transmodulator (6) one ends with perfusate chamber (2), the other end of perfusate chamber (2) is divided into two-way by first channel transmodulator (4), leading up to second passage transmodulator (5) and waterproof type peristaltic pump (1) is communicated with, and another road connects reservoir (3); Reservoir (3) also is connected with second passage transmodulator (5) by four-way transmodulator (7).
2. the supporting bio-reactor of the integrated dynamic inoculation culture of skeletonization seed cell as claimed in claim 1, it is characterized in that, described peristaltic pump (1) is communicated with by third channel transmodulator (6) and perfusate chamber (2), perfusate chamber (2) is communicated with by first channel transmodulator (4) and reservoir (3), and reservoir (3) is communicated with waterproof type peristaltic pump (1) to constitute by second passage transmodulator (5) and four-way transmodulator (7) cultivates loop (11);
Described peristaltic pump (1) is communicated with by third channel transmodulator (6) and perfusate chamber (2), and perfusate chamber (2) is communicated with waterproof type peristaltic pump (1) to constitute by first channel transmodulator (4) and second passage transmodulator (5) inoculates loop (10).
3. the supporting bio-reactor of the integrated dynamic inoculation culture of skeletonization seed cell as claimed in claim 1 or 2 is characterized in that described peristaltic pump (1) is the waterproof type peristaltic pump.
4. the supporting bio-reactor of the integrated dynamic inoculation culture of skeletonization seed cell as claimed in claim 1 or 2 is characterized in that described pipe connecting (9) is the ventilation property pipe.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201120009776 CN201952432U (en) | 2011-01-13 | 2011-01-13 | Osteogenic seeding cell integrated dynamic inoculation culture matched bioreactor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201120009776 CN201952432U (en) | 2011-01-13 | 2011-01-13 | Osteogenic seeding cell integrated dynamic inoculation culture matched bioreactor |
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| Publication Number | Publication Date |
|---|---|
| CN201952432U true CN201952432U (en) | 2011-08-31 |
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| CN 201120009776 Expired - Fee Related CN201952432U (en) | 2011-01-13 | 2011-01-13 | Osteogenic seeding cell integrated dynamic inoculation culture matched bioreactor |
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| CN (1) | CN201952432U (en) |
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2011
- 2011-01-13 CN CN 201120009776 patent/CN201952432U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| ASS | Succession or assignment of patent right |
Owner name: THE 4TH MILITARY SURGEON UNIV. P. L. A. Free format text: FORMER OWNER: WANG LIN Effective date: 20140930 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20140930 Address after: 710032 Changle West Road, Shaanxi, China, No. 17, No. Patentee after: The Fourth Military Medical University of the Chinese People's Liberation Army Address before: 710032 Changle West Road, Shaanxi, China, No. 17, No. Patentee before: Wang Lin |
|
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20110831 Termination date: 20150113 |
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| EXPY | Termination of patent right or utility model |