CN201883093U - Multi-level membrane sampler for deep-sea microorganism - Google Patents

Multi-level membrane sampler for deep-sea microorganism Download PDF

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Publication number
CN201883093U
CN201883093U CN 201020599418 CN201020599418U CN201883093U CN 201883093 U CN201883093 U CN 201883093U CN 201020599418 CN201020599418 CN 201020599418 CN 201020599418 U CN201020599418 U CN 201020599418U CN 201883093 U CN201883093 U CN 201883093U
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China
Prior art keywords
sea
deep
plunger
filtration
collection head
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Expired - Fee Related
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CN 201020599418
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Chinese (zh)
Inventor
陶春辉
周建平
顾临怡
邓显明
顾春华
李怀明
何拥华
张金辉
吴新然
钱鑫炎
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Second Institute of Oceanography SOA
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Second Institute of Oceanography SOA
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Priority to CN 201020599418 priority Critical patent/CN201883093U/en
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Abstract

The utility model discloses a deep-sea mechanical device, in particular a sampler for deep-sea microorganisms, which comprises a telescopic collection head, a three-plunger sea water pump and a multi-level membrane filtration and pressure maintaining sample container. A direct current brushless motor is connected with the three-plunger sea water pump, the inlet of the three-plunger sea water pump is connected with suction pipes of the collection head, the outlet of the three-plunger sea water pump is connected with a six-way adapter pipe, five outlets of the six-way adapter pipe are respectively connected with a group of two-level membrane filtration and pressure maintaining sample container, the inlet of each group of two-level membrane filtration and pressure maintaining sample container is connected with a one-way valve, the outlet of each group of two-level membrane filtration and pressure maintaining sample container is connected with an electromagnetic one-way valve, and the electromagnetic one-way valves are directly connected with sea water. The sampler provided by the utility model has the advantages that time can be accurately controlled, the plunger pump flow is stable, the work pressure is high, the sampler can adapt to a multi-level filtering membrane filter system, the collection head can be protected, and spot sampling can also be realized. The multi-level membrane sampler for the deep-sea microorganism provided by the utility model can be widely used in ocean research and collection industry.

Description

The multistage film sampling unit of a kind of Deep-Sea Microorganisms
Technical field
The utility model relates to a kind of deep-sea mechanism, specifically is meant a kind of sampling unit that is used for Deep-Sea Microorganisms.
Technical background
For the research of seabed microorganism, the most frequently used method is still fetched sample from the seabed and is studied to scientific surveying ship.Concentrate the sample mode of sampling, utilize the suction of equipment and filter design to make seawater enter sampler exactly and sample, promptly belong to initiatively sampling.
Seabed microorganism sampler generally is the single-stage filtering system at present, and the microbiological specimens impurity of obtaining is more, and research and analysis are produced a very large impact, and therefore is necessary to adopt multistage membrane filtration system.
The pump that the volume-variation of utilizing plunger plunger hole in the to-and-fro movement parallel with transmission shaft to be produced is carried out work is called ram pump, compares with other pumps, and the advantage that ram pump has has: the volumetric efficiency height, running steadily, discharge uniformity is good, and noise is low, the operating pressure height.In the sampling by filtration of Deep-Sea Microorganisms, the hole of filtering membrane is very little, needs bigger filtration pressure difference, and Deep-Sea Microorganisms concentration is very low in addition, often need to filter the amount that the more water yield just can be obtained needs, so flow is definite very important to studying its concentration.These can be resolved by ram pump.
Adopt the transistor commutating circuit to replace the motor of brush and reverser to be called dc brushless motor.Dc brushless motor is compared the common electric machine advantage to be had: the rotating speed variable range is big, and the motor speed of enclosed control is stable, is subjected to load effect very little.
Sampler is transferred to after the deep-sea with framework; the collection head suction pipe need be reached framework samples outward; and in transferring process in order to protect the collection head suction pipe, be with in the collection head suction pipe income framework, therefore the design collection head that can stretch also is necessary for the deep earth sampling device.
After sampling finishes, the gastight sampling tube can be put forward back sea level with framework under the effect of hawser, and in the process of this rising, the pressure of pressurize tube outside is in continuous reduction, every rising 100m then can fall 1MPa, final after the pressurize tube is mentioned sea level, the pressure of pressurize tube outside reduces to zero, but its inside has still kept the original position pressure of sampling spot.The pressurize cylindrical shell because recoverable deformation produces volumetric expansion, is added the distortion of other elastomericss (as sealing member etc.) in the effect meeting of interior pressure, and system is difficult to reach the pressurize effect of expection, therefore also needs to realize half initiatively pressurize by the pressure boost compensation system.
Deep-Sea Microorganisms living environment pressure is very high, and the sample that sampling obtains will remain under the highly compressed environment, otherwise will be because of decompression death, and how to shift microorganism under the highly compressed condition also is a problem of comparison difficulty in follow-up cultivation or other equipment.
The utility model content
The utility model has proposed the multistage film sampling unit of a kind of Deep-Sea Microorganisms at deficiency of the prior art.
In order to overcome the above problems, the utility model is to be achieved through the following technical solutions:
The multistage film sampling unit of a kind of Deep-Sea Microorganisms comprises flexible collection head, and three plunger sea-water pumps and multistage membrane filtration gastight sampling tube is characterized in that:
Dc brushless motor is connected with the plunger sea-water pump, and the ingress of three plunger sea-water pumps is connected with the collection head suction pipe, the exit connects six logical transfer tubes; Five outlets of six logical transfer tubes connect one group of two-stage membrane filtration pressurize tube respectively, and the outlet that the import of every group of filtration pressurize tube is connected with check valve, filtration pressurize tube is connected with the electromagnetism check valve, and magnetic valve leads directly to seawater; Deep ocean work is the human difficult field that relates to always, in order to realize the good effect of dark liquid operation, must realize mechanically.The utility model must design a kind of specific device in order to collect corresponding microorganism at dark liquid, can be at the deep-sea long-play.So the utility model is provided with a plurality of collection head suction pipes, like this can be in the deep ocean work longer time; All need a lot of hour otherwise under device, get back to the sea, waste more times to the deep-sea and from the deep-sea; For this reason, the utility model designs five groups and filters the pressurize tube, can be more economical; But, then do not have reliability from economic angle, power configuration etc. if the pressurize tube is filtered in many again being provided with.Simultaneously, in order to collect Wei Shengte better at the deep-sea, need continuous filtering sea, at first the more smart Impurity removal in the seawater, promptly filter the pressurize tube by one-level, filtering membrane aperture wherein is bigger, the mean pore size of general first step film is 5 millimeters, filters the deep-sea suspended substance of larger particles; The mean pore size of second stage film is 1.5~2.5 millimeters, is used for filtering microorganism, certainly, in this device, if the more special microorganism of collection, also can adjust membrane pore size wherein.
As preferably, collection head in the said apparatus all is connected with dc brushless motor with the plunger sea-water pump, gather microorganism at the deep-sea, in order to obtain enough amounts, need the long time, need constantly seawater to be filtered, little living spy is retained down, and employing plunger sea-water pump etc. are comparatively steady in operational process, and it is injury-free to help microorganism.
As preferably, five groups are filtered pressurize tube, ram pump and the independent frame fixation of flexible collection head use in the said apparatus, all adopt hose connection between the suction pipe of pump and filtration pressurize tube, pump and flexible collection head, has very big pressure at the deep-sea, general device all can't normally move at the deep-sea, in order to ensure the safety of device, guarantee that device can normally use so in device, all be provided with frame fixation separately.
As preferably, be connected with three-way cut-off valve between the two-stage filtration film in the said apparatus, leave an interface on the stopping valve, stopping valve herein is an interface to be opened/closed, can be used for sample is shifted more easily.
As preferably, every group of filtration pressurize tube all is connected with an energy storage in the said apparatus, because the pressure at deep-sea is very big, the microorganism of being gathered in the utility model generally also is all to survive in the state of dark liquid, survival in order to ensure microorganism, so all needing in gatherer process, after the middle collection provides one to be fit to its survival environment to microorganism, and the pressure-controlling of device of the present utility model also is very crucial; If in gatherer process or after collection, leave after the sea,, pressure change causes the death of microorganism possibly because of surpassing environmental factors such as 10%; So in the utility model, and filter the pressurize tube and all be connected an energy storage, the pressure that can regulate the pressure that filters the pressurize tube and deep-sea at any time is suitable, guarantees that the environmental change of microorganism is little.
As preferably, the import of every group of pressurize tube, exit are connected with check valve, electromagnetism check valve respectively in the said apparatus, can realize the sealing of sample, can realize the sealing of sample.
Valve during this is bright comprises valve body, ball face sealing spare, spherical valve core and valve rod.
Beneficial effect is:
(1) the utility model proposes the system that the dc brushless motor that uses closed-loop control drives, can control rotating speed of motor accurately, and rationally set the time of needs running according to rotating speed.
(2) the utility model proposes and utilize ram pump extracting seawater sample, because the ram pump flow is stable, the operating pressure height can adapt to the filtering system of cascade filtration film, and can be according to rotating speed of motor and determine the flow that passes through runtime.
(3) the utility model proposes by two-stage pressurize tube and carry out multistage membrane filtration, the impurity particle that first step film can filtering useless, second stage film can filter out more purified microorganism, can solve the impure problem of microbiological specimens.
(4) the utility model proposes the sampling modes of organizing the parallel connection of pressurize tube more,, can be implemented in the different depth of water and get different samples in conjunction with the control of electromagnetism check valve.
(5) the utility model proposes telescopic collection head suction pipe, both can protect collection head, can realize spot sampling again.
Description of drawings
Fig. 1 is a scalable collection head suction pipe retracted state synoptic diagram of the present utility model.
Fig. 2 is that scalable collection head suction pipe stretches out view.
Fig. 3 is that dc brushless motor and ram pump are installed in the synoptic diagram in the framework.
Fig. 4 is that five groups of sampling pressurize tubes are installed in the synoptic diagram in the framework.
Embodiment
In conjunction with the accompanying drawings, below by specific embodiment the utility model is elaborated.
Embodiment 1
The multistage film sampling unit of a kind of Deep-Sea Microorganisms, comprise flexible collection head 2, three plunger sea-water pumps 3 and multistage membrane filtration pressurize tube 5, dc brushless motor 1 is connected with three plunger sea-water pumps 3, and the ingress of three plunger sea-water pumps 3 is connected with collection head 2, the exit connects six logical transfer tubes 8; Five outlets of six logical transfer tubes 8 connect one group of two-stage membrane filtration pressurize tube 5 respectively, and the outlet that the import of every group of filtration pressurize tube 5 is connected with check valve 4, filtration pressurize tube 5 is connected with electromagnetism check valve 6, and magnetic valve leads directly to seawater.The filtration pressurize tube 5 that three-way cut-off valve 7 is formed and one six logical transfer tube 8, every group is filtered pressurize tube 5 and is connected with an energy storage 9.
6 suction pipes are arranged on the collection head 2, and wherein the end of a suction pipe is connected by the import of flexible pipe with three plunger sea-water pumps 3, and the outlet of three plunger sea-water pumps 3 to six logical transfer tubes 8, forms total system by hose connection.
Collection head 2 is driven by the leading screw that dc brushless motor 1 drives, and from the retracted state setting in motion, moves to the state of stretching out, and is stuck in order to prevent to advance to limit position, by setting rotating speed of motor and controlling the movement travel of collection head runtime.
After collection head 2 reaches specified location, open in five electromagnetism check valves 6, open the dc brushless motor 1 of driving pump then, extracting seawater is taken a sample.By setting rotating speed of motor and runtime, can control flow, and then can know the degree that Deep-Sea Microorganisms is concentrated by the seawater of system.
When needing the replacing position to sample, have dual mode to select: first kind is the mode of not closing three plunger sea-water pumps 3, open that group that to take a sample earlier and filter the electromagnetism check valve 6 of pressurize tube 5, close that group filtration pressurize tube 5 that does not need to take a sample again and get final product; Second kind is that the place or the lag time of two sub-samplings is bigger, sampler need drag to the mode of next sampling spot with framework, that group that need close three plunger sea-water pumps 3 this moment earlier and take a sample is filtered the electromagnetism check valve 6 of pressurize tube 5, again collection head 2 is retracted in the framework, by the time after the next sampling spot, repeat the action of sampling for the first time.
After sampling is finished, close three plunger sea-water pumps 3, filter the electromagnetism check valve 6 of pressurize tube 5, regain collection head 2, sampling process finishes, and sampler can be suggested the sea with framework.In the process that rises, along with the reduction of ambient pressure environment, filter pressurize tube 5 pressure inside and be higher than ambient pressure, filtration pressurize tube 5 deforms and causes cylinder pressure to reduce, this moment, ftercompction water was carried in energy storage 9 meetings in pressurize tube 5, and the pressure in the pressurize tube 5 are maintained in the scope of permission.
The way of taking out sample is: the outlet of electromagnetism check valve 6 is connected high-pressure pump, the interface of three-way cut-off valve 7 is connected to other equipment with kapillary, utilize high-pressure pump in high pressure cylinder, to charge into distilled water by electromagnetism check valve 6, the sample of microorganism can be from the exit of three-way cut-off valve 7 under the impact of distilled water, along with current flow in the follow-up equipment, for other research.
At last, it should be noted that above what enumerate only is specific embodiment of the utility model.Obviously, the utility model is not limited to above embodiment, and a lot of distortion can also be arranged.For example the quantity of placed in-line filtering membrane can be three or more, and pressurize tube in parallel can be four groups or other quantity, and the plunger of pump can be single or other quantity.All distortion that those of ordinary skill in the art can directly derive or associate from the disclosed content of the utility model all should be thought protection domain of the present utility model.

Claims (7)

1. the multistage film sampling unit of Deep-Sea Microorganisms comprises flexible collection head (2), and three plunger sea-water pumps (3) and multistage membrane filtration pressurize tube (5) is characterized in that:
Dc brushless motor (1) is connected with three plunger sea-water pumps (3), and the ingress of three plunger sea-water pumps (3) is connected with collection head (2) suction pipe, the exit connects six logical transfer tubes (8); Five outlets of six logical transfer tubes (8) connect one group of two-stage membrane filtration pressurize tube (5) respectively, the outlet that the import of every group of filtration pressurize tube (5) is connected with check valve (4), filtration pressurize tube (5) is connected with electromagnetism check valve (6), and electromagnetism check valve (6) leads directly to seawater.
2. the multistage film sampling unit of a kind of Deep-Sea Microorganisms according to claim 1 is characterized in that, described collection head (2) all is connected with dc brushless motor (1) with three plunger sea-water pumps (3).
3. the multistage film sampling unit of a kind of Deep-Sea Microorganisms according to claim 1, it is characterized in that, described five groups are filtered pressurize tube (5), three plunger sea-water pumps (3) and the independent frame fixation of flexible collection head (2) use, all adopt hose connection between the suction pipe of three plunger sea-water pumps (3) and filtration pressurize tube (5), three plunger sea-water pumps (3) and flexible collection head (2).
4. the multistage film sampling unit of a kind of Deep-Sea Microorganisms according to claim 1, the mode that it is characterized in that the filtration employing two-stage filtration film cascade of described filtration pressurize tube (5), filtering membrane pass through the aperture difference, the mean pore size of first step film is 5 millimeters, and the mean pore size of second stage film is 1.5~2.5 millimeters.
5. the multistage film sampling unit of a kind of Deep-Sea Microorganisms according to claim 4 is characterized in that being connected with between the two-stage filtration film three-way cut-off valve (7), leaves an interface on the three-way cut-off valve (7).
6. the multistage film sampling unit of a kind of Deep-Sea Microorganisms according to claim 1 is characterized in that described every group of filtration pressurize tube (5) all is connected with an energy storage (9).
7. the multistage film sampling unit of a kind of Deep-Sea Microorganisms according to claim 6 is characterized in that the import of described every group of pressurize tube (5), exit are connected with check valve (4), electromagnetism check valve (6) respectively.
CN 201020599418 2010-11-10 2010-11-10 Multi-level membrane sampler for deep-sea microorganism Expired - Fee Related CN201883093U (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102108331A (en) * 2010-11-10 2011-06-29 国家海洋局第二海洋研究所 Deep sea microorganism multilevel membrane sampling device
CN109030112A (en) * 2018-07-03 2018-12-18 浙江省海洋水产研究所 Construction time flotage monitoring system

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102108331A (en) * 2010-11-10 2011-06-29 国家海洋局第二海洋研究所 Deep sea microorganism multilevel membrane sampling device
CN109030112A (en) * 2018-07-03 2018-12-18 浙江省海洋水产研究所 Construction time flotage monitoring system
CN109030112B (en) * 2018-07-03 2020-12-11 浙江省海洋水产研究所 Construction period suspended solid monitoring system

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C17 Cessation of patent right
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Granted publication date: 20110629

Termination date: 20121110