CN201852770U - Device for counting blood platelets - Google Patents
Device for counting blood platelets Download PDFInfo
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- CN201852770U CN201852770U CN2010205514530U CN201020551453U CN201852770U CN 201852770 U CN201852770 U CN 201852770U CN 2010205514530 U CN2010205514530 U CN 2010205514530U CN 201020551453 U CN201020551453 U CN 201020551453U CN 201852770 U CN201852770 U CN 201852770U
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- platelet
- platelet count
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- penetrability
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Abstract
The utility model provides a simple device for quickly counting blood platelets and belongs to the field of medical biotechnology. The device is characterized in that: an instrument for determining blood platelet aggregation is additionally provided with a turbidimetric tube (''standard tube'' for short) with fixed transmittance which is a fixed valve between 22% and 38%. In the instrument for determining blood platelet aggregation, the standard tube is tuned to 100%, poor blood platelet plasma is tuned to 0, and the standard tube is replaced with a testing tube of platelet rich plasma (PRP) to be tested to measure and obtain the relative concentration of blood platelets in the PRP; and the relative concentration of blood platelets in the PRP is multiplied by the count of blood platelets corresponding to the standard tube to obtain the count of platelets of the PRP to be tested.
Description
Technical field
The utility model relates to the medicine bioengineering Instrument technology field, relates in particular to a kind of device of platelet count.
Background technology (bibliography E.D.Olsen, Modern Optical Methods of Analysis, McGraw-Hill, New York, 1975. Liu's books are intelligent, Wang Jingzi. the improvement of platelet count method. the journal .1984 of Shandong medical college, 22 (4): 81-83)
Platelet aggregation test is an important experiment estimating platelet function, and is closely related with cardiovascular and cerebrovascular disease.What the principle of work of platelet aggregation instrument adopted is turbidimetry, promptly measures the method that the light intensity that sees through the particle in suspension medium is determined suspended material concentration.Adopt turbidimetry can determine to be rich in hematoblastic concentration in the blood platelet blood plasma (PRP), in platelet aggregation instrument, represent with relative concentration 100%.When adding derivant (as ADP etc.) among the PRP, blood platelet is assembled gradually, and the particle in suspension medium reduces, and blood plasma is clarified gradually, and penetrability increases thereupon.Can calculate the variation of PC according to the change of penetrability.The variation of PC is the platelet counts of gathering, represents with platelet aggregation rate in platelet aggregation instrument.Hematoblastic relative concentration behind platelet aggregation rate=PRP adding derivant thromboblast relative concentration 100%-PRP adding derivant.In platelet aggregation test, require the interior platelet count that is rich in blood platelet blood plasma (PRP) of opacity tube 30 * 10
4/ mm
3About be advisable.The homemade platelet aggregation instrument of part and most import platelet aggregation instrument do not have the platelet count function, need to adopt blood cell analyzer to detect platelet counts.Can increase Experimental Establishment like this, improve experimental cost.Domestic most laboratory is failed to platelet aggregation instrument is equipped with blood cell analyzer specially, but the method that adopts conventional microscope to detect.Utilize common blood count dish, detect platelet counts at microscopically and can expend many time and efforts.The import platelet aggregation instrument is favored by many experimenters with its high precision, and the platelet count problem that solves these instruments will be brought great convenience for operating personnel.
Summary of the invention
In order to overcome the deficiency in the above-mentioned background technology, the utility model is for a kind of simple and practical platelet count device is provided, and in order to realize the purpose of foregoing invention, the utility model adopts following technical scheme:
1. in the subsidiary at random opacity tube of platelet aggregation instrument, place a translucent shade (as frosted glass plate of vertical fixing or evenly fill various gelatinous mass), making its penetrability under 485nm wavelength light is 22～38%, and it is referred to as standard pipe.
2. utilize the negative correlativing relation that exists between platelet count and the penetrability, calculate the platelet count that standard pipe is represented according to typical curve or formula.
3. standard pipe (the concrete platelet count of known representative) is positioned in the platelet aggregation analyzer and transfers 100%, promptly hematoblastic relative concentration is 100%; Transfer 0 with platelet poor plasma (PPP) pipe, promptly hematoblastic relative concentration is 0.Replace standard pipe with blood platelet blood plasma (PRP) testing tube that is rich in to be measured again, can record a percentage, instrument is shown as " platelet aggregation rate ", actual for the standard pipe representative blood platelet relative concentration 100% and PRP in the difference of blood platelet relative concentration.Hematoblastic relative concentration=1-" platelet aggregation rate " among the PRP.The platelet count of hematoblastic relative concentration * standard pipe correspondence among the PRP is the platelet count that is rich in blood platelet blood plasma to be measured.
Description of drawings
Fig. 1 is a platelet count apparatus structure synoptic diagram;
1, connect computer 2, light source 3, to transfer 0 position 4, content be that PPP 5, standard pipe position/accent 100% position 6, content are translucent shade
Fig. 2 is a standard pipe synoptic diagram of vertically placing frosted glass plate;
2, light source 7, frosted glass plate
Fig. 3 is the standard pipe synoptic diagram of gel filled material;
2, light source 8, gelatinous mass
Embodiment
Embodiment 1
1. the making of blood platelet typical curve
Get the rat fresh blood, the anti-freezing of tangerine rafter acid sodium, anti-coagulants and blood ratio are 1: 9, centrifugal (200 * g, 10min) separate PRP, get PRP centrifugal again (1200 * g, 10min), blood platelet, with tyrode's solution suspension blood platelet, make platelet suspension, and counting is transferred to 62.5 * 10 again
4/ mm
3Again with tyrode's solution in 10: 8 ratio proportional diluted, obtain the platelet suspension of 6 concentration, and detect platelet count at microscopically.Every kind of suspension under 485nm wavelength light, transfers 0 with the distillation water pipe respectively with 721 spectrophotometers, measures penetrability.The results are shown in Table 1.
Table 1 penetrability and platelet count corresponding relation
Related coefficient: r=-0.9867, the slope of regression line: a=-1.6051, intercept: b=94.4620
2. the preparation of standard pipe
Choose a frosted glass, it is cut into the wide opacity tube internal diameter that is, length is to be vertically fixed on a rectangle of opacity tube height in the opacity tube.This opacity tube is placed 721 spectrophotometers, make frosted glass plate vertical with light, under 485nm wavelength light, transfer 0 with the distillation water pipe, measure penetrability, penetrability is 22%, calculates according to typical curve, and being equivalent to platelet count is 590,000/mm
3
3. platelet count
Get 10 rat fresh bloods, the anti-freezing of tangerine rafter acid sodium, anti-coagulants and blood ratio are 1: 9, it is centrifugal that (200 * g 10min), separates PRP, in the sky opacity tube of packing into.Remainder is centrifugal, and (1200 * g 10min), gets platelet poor plasma (PPP).
In platelet aggregation instrument, transfer 100% with standard pipe, transfer 0, replace standard pipe with PRP testing tube to be measured again with PPP, record the platelet count of hematoblastic relative concentration among the PRP (being 1-" platelet aggregation rate ") * standard pipe correspondence, be the platelet count of PRP to be measured.Each PRP detects platelet count at microscopically respectively, and it is very little that the result shows that microscopy method and standard pipe contrast computing method difference, learns by statistics and handle no significant difference, sees Table 2,3.
Table 2 blood platelet relative concentration and platelet count corresponding relation
Two kinds of assay method results of table 3 relatively
1. the making of blood platelet typical curve
Use embodiment 1 typical curve.
2. the preparation of standard pipe
Choose a frosted glass, it is cut into the wide opacity tube internal diameter that is, length is to be vertically fixed on a rectangle of opacity tube height in the opacity tube.This opacity tube is placed 721 spectrophotometers, make frosted glass plate vertical with light, under 485nm wavelength light, transfer 0 with the distillation water pipe, measure penetrability, penetrability is 38%, calculates according to typical curve, and being equivalent to platelet count is 33.5 ten thousand/mm
3
3. platelet count
Get 10 rat fresh bloods, the anti-freezing of tangerine rafter acid sodium, anti-coagulants and blood ratio are 1: 9, it is centrifugal that (200 * g 10min), separates PRP, in the sky opacity tube of packing into.Remainder is centrifugal, and (1200 * g 10min), gets platelet poor plasma (PPP).
In platelet aggregation instrument, transfer 100% with standard pipe, transfer 0 with PPP, replace standard pipe with PRP testing tube to be measured again, record " platelet aggregation rate " and be negative value, hematoblastic relative concentration among the PRP (being 1-" platelet aggregation rate ")＞100% is behind PPP dilution PRP, record the platelet count of hematoblastic relative concentration among the PRP (being 1-" platelet aggregation rate ") * standard pipe correspondence, be the platelet count of dilution back PRP.Each PRP detects platelet count at microscopically respectively, and it is very little that the result shows that microscopy method and standard pipe contrast computing method difference, learns by statistics and handle no significant difference.The platelet count of dilution back PRP is at 300,000/mm
3About can be platelet aggregation test and use.The results are shown in Table 4,5.
Table 4 blood platelet relative concentration and platelet count corresponding relation
Two kinds of assay method results of table 5 relatively
Embodiment 3
1. the making of blood platelet typical curve
Use embodiment 1 typical curve.
2. the preparation of standard pipe
The aqueous agar solution that in opacity tube, adds heating for dissolving, put cold make its become gel (various colloidal materials can be as the material of production standard pipe, as gelatin, Al (OH)
3Colloid, Fe (OH)
3Colloid etc.But with agar is preferred.)。This opacity tube is placed 721 spectrophotometers, make light vertical with opacity tube, under 485nm wavelength light, transfer 0 with the distillation water pipe, measure penetrability, penetrability is 22%, calculates according to typical curve, and being equivalent to platelet count is 590,000/mm
3
3. platelet count
Get 10 rat fresh bloods, the anti-freezing of tangerine rafter acid sodium, anti-coagulants and blood ratio are 1: 9, it is centrifugal that (200 * g 10min), separates PRP, in the sky opacity tube of packing into.Remainder is centrifugal, and (1200 * g 10min), gets platelet poor plasma (PPP).
In platelet aggregation instrument, transfer 100% with standard pipe, transfer 0, replace standard pipe with PRP testing tube to be measured again with PPP, record the platelet count of hematoblastic relative concentration among the PRP (being 1-" platelet aggregation rate ") * standard pipe correspondence, be the platelet count of PRP to be measured.Each PRP detects platelet count at microscopically respectively, and it is very little that the result shows that microscopy method and standard pipe contrast computing method difference, learns by statistics and handle no significant difference, sees Table 6,7.
Table 6 blood platelet relative concentration and platelet count corresponding relation
Two kinds of assay method results of table 7 relatively
Conclusion: the standard pipe penetrability is in 22～38% requirements that can satisfy platelet count in the platelet aggregation test.
Claims (7)
1. a platelet count device is characterized in that on platelet aggregation analyzer basis, increases by one and has the fixedly opacity tube of penetrability, can represent the platelet count that has in the identical penetrability blood plasma.
2. by the described platelet count device of claim 1, it is characterized in that: what contain in this device has a fixedly opacity tube of penetrability, and the penetrability under 485nm wavelength light is a fixed value between 22～38%.
3. by the described platelet count device of claim 2, it is characterized in that: what contain in this device has a fixedly method for making of the opacity tube of penetrability, be in the subsidiary at random opacity tube of platelet aggregation instrument, to place a translucent shade, make the penetrability of opacity tube under 485nm wavelength light between 22～38%.
4. by the described platelet count device of claim 3, it is characterized in that: the translucent shade of use is that a vertical fixing is in opacity tube and the frosted glass plate vertical with light source in this device.
5. by the described platelet count device of claim 3, it is characterized in that: the translucent shade that uses in this device is a gelatinous mass.
6. by the described platelet count device of claim 5, it is characterized in that: the gelatinous mass that uses in this device is agar, gelatin, Al (OH)
3Colloid, Fe (OH)
3Colloid.
7. by the described platelet count device of claim 6, it is characterized in that: the gelatinous mass that uses in this device is preferably agar.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010205514530U CN201852770U (en) | 2010-09-30 | 2010-09-30 | Device for counting blood platelets |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010205514530U CN201852770U (en) | 2010-09-30 | 2010-09-30 | Device for counting blood platelets |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201852770U true CN201852770U (en) | 2011-06-01 |
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ID=44095159
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010205514530U Expired - Fee Related CN201852770U (en) | 2010-09-30 | 2010-09-30 | Device for counting blood platelets |
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| Country | Link |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110308271A (en) * | 2019-07-10 | 2019-10-08 | 江苏柯伦迪医疗技术有限公司 | A kind of platelet function assay system and detection method |
-
2010
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110308271A (en) * | 2019-07-10 | 2019-10-08 | 江苏柯伦迪医疗技术有限公司 | A kind of platelet function assay system and detection method |
| WO2021003786A1 (en) * | 2019-07-10 | 2021-01-14 | 江苏柯伦迪医疗技术有限公司 | Platelet function detection system and detection method |
| CN110308271B (en) * | 2019-07-10 | 2021-03-30 | 江苏柯伦迪医疗技术有限公司 | Platelet function detection system and detection method |
| GB2598865A (en) * | 2019-07-10 | 2022-03-16 | Sinnowa Medical Science & Tech Co Ltd | Platelet function detection system and detection method |
| GB2598865B (en) * | 2019-07-10 | 2022-07-20 | Sinnowa Medical Science & Tech Co Ltd | Platelet function detection system and detection method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20110601 Termination date: 20160930 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |