CN201704333U - Enzyme reactor applicable to high-viscosity liquid system - Google Patents

Enzyme reactor applicable to high-viscosity liquid system Download PDF

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Publication number
CN201704333U
CN201704333U CN 201020206637 CN201020206637U CN201704333U CN 201704333 U CN201704333 U CN 201704333U CN 201020206637 CN201020206637 CN 201020206637 CN 201020206637 U CN201020206637 U CN 201020206637U CN 201704333 U CN201704333 U CN 201704333U
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China
Prior art keywords
valve
enzymatic vessel
enzyme
reaction
circulation pipe
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Expired - Fee Related
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CN 201020206637
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Chinese (zh)
Inventor
夏文水
许学勤
白云鹏
张家骊
姜启兴
梁双林
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Jiangsu Shuanglin Marin Biological Phamacuetic Co Ltd
Jiangnan University
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Jiangsu Shuanglin Marin Biological Phamacuetic Co Ltd
Jiangnan University
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Priority to CN 201020206637 priority Critical patent/CN201704333U/en
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02Stirrer or mobile mixing elements
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00Bioreactors or fermenters specially adapted for specific uses
    • C12M21/18Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/18External loop; Means for reintroduction of fermented biomass or liquid percolate

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The utility model discloses an enzyme reactor applicable to a high-viscosity liquid system. A stirrer is disposed inside an enzymolysis tank, a solid reagent feed inlet is arranged on the enzymolysis tank, the upper portion of the enzymolysis tank is respectively connected with a first reaction solvent feeding valve, a compressed air valve and a process water valve, the enzymolysis tank is connected with a circulation pipeline device, the circulation pipeline device includes a second circulation pipe valve connected with the upper portion of the enzymolysis tank, the second circulation pipe valve is connected with a high shear homogenizer, the high shear homogenizer is connected with a first circulation pipe valve, a second reaction solvent feeding valve connected with a second reaction solvent tank is connected to a pipeline between the high shear homogenizer and the first circulation pipe valve, the first circulation pipe valve is connected with an unloading valve, the unloading valve is connected with the lower portion of the enzymolysis tank, and a discharge valve and a drain valve are connected to a pipeline between the first circulation pipe valve and the unloading valve. The enzyme reactor is reasonable in structure, uniformly scatters enzyme engaged in reaction into the high-viscosity liquid system and leads the whole enzyme reaction process to be smoothly undertaken.

Description

Be applicable to the enzyme reactor of high viscosity feed liquid system
Technical field:
The utility model relates to a kind of enzyme reactor.
Background technology:
In the biological enzyme reaction, substrate is a key factor of guaranteeing that enzyme reaction is efficiently carried out with fully contacting of enzyme.Yet the polymer substrate of some high stickinesses is because the characteristics solvability of himself physico-chemical property is relatively poor.Existing enzyme reactor only mostly is the reactor with low-speed agitator.This class reactor only is applicable to the low-viscosity feed liquid system of good fluidity.For the very big feed liquid system of viscosity, the enzyme of reaction usefulness can not obtain disperseing quickly and evenly, from not obtaining enzyme reaction effect efficiently, if the local product that produces of enzyme digestion reaction has restraining effect to enzyme, then might make the part enzymic activity.
Summary of the invention:
It is a kind of rational in infrastructure that the purpose of this utility model is to provide, and the enzyme that participation is reacted is dispersed in the enzyme reactor that is applicable to high viscosity feed liquid system in the high viscosity solution system full and uniformly.
Technical solution of the present utility model is:
A kind of enzyme reactor that is applicable to high viscosity feed liquid system, it is characterized in that: comprise enzymatic vessel, agitator is set in the enzymatic vessel, the solid reactants dog-house is set on the enzymatic vessel, enzymatic vessel top respectively with the first solvent feed valve, compressed air valve and technology water valve connect, enzymatic vessel is connected with the circulating line device, the circulating line device comprises second cycle tube valve that is connected with enzymatic vessel top, second cycle tube valve is connected with high-shear homogenizing machine, high-shear homogenizing machine is connected with first cycle tube valve, connect one and the tank connected second reaction solvent material valve of second reaction solvent on the pipeline between the high-shear homogenizing machine and first cycle tube valve, first cycle tube valve is connected with baiting valve, baiting valve is connected with the enzymatic vessel bottom, is connected to bleeder valve and wash water valve on the pipeline between first cycle tube valve and the baiting valve.
Enzymatic vessel is the chuck heat form, and chuck is provided with the chuck constent temperature heater.
The utility model is rational in infrastructure, high-shear homogenizing machine and the coupling of enzyme reaction jar are formed a kind of intermittent type enzyme reactor, the enzyme that participates in reaction is dispersed in the high viscosity solution system full and uniformly, the product that reaction process produces can in time be distributed to whole solution system, makes whole enzyme reaction process carry out smoothly.
Description of drawings:
The utility model is described in further detail below in conjunction with drawings and Examples.
Fig. 1 is the configuration diagram of an embodiment of the utility model.
Embodiment:
A kind of enzyme reactor that is applicable to high viscosity feed liquid system, comprise enzymatic vessel 12, agitator 9 is set in the enzymatic vessel, solid reactants dog-house 11 is set on the enzymatic vessel, enzymatic vessel top respectively with the first solvent feed valve 7, compressed air valve 8 and technology water valve 10 connect, enzymatic vessel is connected with the circulating line device, the circulating line device comprises second cycle tube valve 6 that is connected with enzymatic vessel top, second cycle tube valve is connected with high-shear homogenizing machine 1, high-shear homogenizing machine is connected with first cycle tube valve 16, connect second a reaction solvent material valve 2 that is connected with the second reaction solvent jar 3 on the pipeline between high-shear homogenizing machine and first cycle tube valve, first cycle tube valve is connected with baiting valve 15, baiting valve is connected with the enzymatic vessel bottom, is connected to bleeder valve 18 and wash water valve 17 on the pipeline between first cycle tube valve and the baiting valve.
Enzymatic vessel is the chuck heat form, and chuck is provided with chuck constent temperature heater 13.
Working process of the present utility model is:
Enzyme reaction is after at first connecting pipeline is put into the process water of an amount by the technology water valve, open the agitator 9 of enzymatic vessel 12, simultaneously by solid reactants dog-house 11, drop into enzyme reaction solid substrate and zymin in proportion, the enzyme reaction solid substrate that drops into and zymin and reaction solvent one are mixed with finely dispersed suspension.Open baiting valve 15, second cycle tube valve 6 and first cycle tube valve 16, open high-shear homogenizing machine 1 simultaneously,, further improve jar dispersiveness and the suspension stability of inner suspension feed liquid by the circulation shear effect.By predetermined proportion with the reaction solvent two second reaction solvent jar 3 of packing into, open the second reaction solvent material valve 2, make reaction solvent two with certain proportion with just mix at round-robin suspension, under 1 effect of high shear mixing clarifixator, make reaction solvent two fully contact dispersion with suspension, simultaneously with enzyme reaction solid substrate dissolving in the suspension, become full-bodied solution, to the last obtaining a kind of zymin is dispersed in (by solvent one, solvent two and dissolved enzyme reaction solids constitute) the enzyme reaction system of high viscosity substrate solution, close second cycle tube valve 6, first cycle tube valve 16, high-shear homogenizing machine 1.Agitator 9 continues operation, finishes up to enzyme reaction.After reaction is finished, close solid reactants dog-house 11, agitator 9, the first solvent feed valve 7, open bleeder valve 18, open compressed air valve 8 then, the feed liquid that makes the reaction of having finished in the constant temperature stirred pot is delivered to the next procedure of this enzyme reactor by bleeder valve 18 back pipelines.
After treating that feed liquid drains from enzymatic vessel 12 fully, close bleeder valve 18, open technology water valve 10 and make process water enter enzymatic vessel 12, and open second cycle tube valve 6, first cycle tube valve 16, high-shear homogenizing machine 1 and agitator 9, whole enzyme reactor is cleaned.Cleaning finishes, and opens wash water valve 17 the jar cleaning liquid inside is drained, and so finishes a clean cycle.
The system temperature of reaction process is realized by chuck constent temperature heater 13.Liquidometer 5, pH meter 4, thermometer 14 are respectively applied for liquid level, pH, the temperature of index enzymatic vessel 12 endoenzyme reaction systems.

Claims (2)

1. enzyme reactor that is applicable to high viscosity feed liquid system, it is characterized in that: comprise enzymatic vessel, agitator is set in the enzymatic vessel, the solid reactants dog-house is set on the enzymatic vessel, enzymatic vessel top respectively with the first solvent feed valve, compressed air valve and technology water valve connect, enzymatic vessel is connected with the circulating line device, the circulating line device comprises second cycle tube valve that is connected with enzymatic vessel top, second cycle tube valve is connected with high-shear homogenizing machine, high-shear homogenizing machine is connected with first cycle tube valve, connect one and the tank connected second reaction solvent material valve of second reaction solvent on the pipeline between the high-shear homogenizing machine and first cycle tube valve, first cycle tube valve is connected with baiting valve, baiting valve is connected with the enzymatic vessel bottom, is connected to bleeder valve and wash water valve on the pipeline between first cycle tube valve and the baiting valve.
2. the enzyme reactor that is applicable to high viscosity feed liquid system according to claim 1 is characterized in that: enzymatic vessel is the chuck heat form, and chuck is provided with the chuck constent temperature heater.
CN 201020206637 2010-05-28 2010-05-28 Enzyme reactor applicable to high-viscosity liquid system Expired - Fee Related CN201704333U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN 201020206637 CN201704333U (en) 2010-05-28 2010-05-28 Enzyme reactor applicable to high-viscosity liquid system

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Application Number Priority Date Filing Date Title
CN 201020206637 CN201704333U (en) 2010-05-28 2010-05-28 Enzyme reactor applicable to high-viscosity liquid system

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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102604828A (en) * 2011-01-24 2012-07-25 上海众伟生化有限公司 Double-body circulatory cellulose enzymatic hydrolysis reactor and application thereof
CN102851209A (en) * 2012-06-25 2013-01-02 沈阳农业大学 Thermostatic stirring solid-state enzymolysis equipment and application method thereof
CN102876571A (en) * 2011-07-12 2013-01-16 上海众伟生化有限公司 High-capacity double-systematic-circulation cellulose enzymolysis reactor and its application
CN106072410A (en) * 2016-08-17 2016-11-09 无穷食品有限公司 A kind of salt local flavor chicken juice process units and production method
CN107568748A (en) * 2017-09-30 2018-01-12 广东生和堂健康食品股份有限公司 A kind of system of processing and its application method of coconut juice beverage
CN107619777A (en) * 2016-12-09 2018-01-23 济南航晨生物科技有限公司 A kind of high-viscosity products dedicated liquid fermentation tank

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102604828A (en) * 2011-01-24 2012-07-25 上海众伟生化有限公司 Double-body circulatory cellulose enzymatic hydrolysis reactor and application thereof
WO2012100510A1 (en) * 2011-01-24 2012-08-02 上海众伟生化有限公司 Dual body circulatory reactor for cellulose enzymolysis and application thereof
CN102604828B (en) * 2011-01-24 2015-02-25 上海众伟生化有限公司 Double-body circulatory cellulose enzymatic hydrolysis reactor and application thereof
CN102876571A (en) * 2011-07-12 2013-01-16 上海众伟生化有限公司 High-capacity double-systematic-circulation cellulose enzymolysis reactor and its application
CN102876571B (en) * 2011-07-12 2014-11-05 上海众伟生化有限公司 High-capacity double-systematic-circulation cellulose enzymolysis reactor and its application
CN102851209A (en) * 2012-06-25 2013-01-02 沈阳农业大学 Thermostatic stirring solid-state enzymolysis equipment and application method thereof
CN106072410A (en) * 2016-08-17 2016-11-09 无穷食品有限公司 A kind of salt local flavor chicken juice process units and production method
CN107619777A (en) * 2016-12-09 2018-01-23 济南航晨生物科技有限公司 A kind of high-viscosity products dedicated liquid fermentation tank
CN107568748A (en) * 2017-09-30 2018-01-12 广东生和堂健康食品股份有限公司 A kind of system of processing and its application method of coconut juice beverage

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Granted publication date: 20110112

Termination date: 20150528

EXPY Termination of patent right or utility model