CN201653904U - Full-automatic optical system for penta-clustering blood analyzer - Google Patents
Full-automatic optical system for penta-clustering blood analyzer Download PDFInfo
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- CN201653904U CN201653904U CN2010201711987U CN201020171198U CN201653904U CN 201653904 U CN201653904 U CN 201653904U CN 2010201711987 U CN2010201711987 U CN 2010201711987U CN 201020171198 U CN201020171198 U CN 201020171198U CN 201653904 U CN201653904 U CN 201653904U
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Abstract
The utility model relates to a full-automatic optical system for a penta-clustering blood analyzer, which is characterized by consisting of a red laser, reflecting lenses, receiving lens cones, aligning lens cones, a flow box and a photoelectric detector, wherein the flow box [P] is arranged between the receiving lens cones [M1] and [M2]; the flow box [P] and the receiving lens cones [M1 and [M2] share the same optical axis; the reflecting lens [C] is arranged outside the receiving lens cone [M1] at an angle of 45 degrees; the incident optical axis of the reflecting lens [C] is coaxial with the receiving lens cones [M1]; the reflecting lenses [D] and [E] are arranged outside the receiving lens cone [M2] at an angle of 45 degrees, respectively; the reflecting optical axis of the reflecting lenses [D] and [E] are coaxial with the receiving lens cone [M2]; the aligning lens cone [L1] is arranged on the reflecting optical axis of the reflecting lens [C] and a photoelectric detector [Q1] is arranged behind the aligning lens cone [L1]; the aligning lens cone [L2] is arranged on the reflecting optical axis of the reflecting lens [D] and a photoelectric detector [Q2] is arranged behind the aligning lens cone [L2]; the red laser [R] is arranged outside the aligning lens cone [L2] and the optical axis thereof is coaxial with the incident optical axis of the reflecting lens [E]; and all components are arranged on a bottom plate [F]. The utility model has the advantages of simple structure, no sheath flow liquid, no fluorescent staining and no other special reagents.
Description
Technical field
The utility model belongs to the optical system of in-vitro diagnosis equipment, relates to full automatic five grouping blood analyser.
Background technology
Current, five grouping blood analyser is divided into five cardinal principles of hiving off with leucocyte to be had following several:
1, mainly adopts the VCS technology
Three kinds of assay methods of this technology set carry out the method for multi parameter analysis simultaneously in one to a cell.Wherein V represents cubing, and promptly traditional electric-resistivity method principle can be separated significant lymphocyte of volume difference in size and granulocyte; C represents high frequency conductance, this technology can directly be measured the interstructural difference of cell interior, understand cell interior caryoplasm ratio and cell internalizing and learn composition, can distinguish the cell of the identical and inner different in kind of cell volume, lymphocyte and basicyte that can volume is close distinguish, because their N/C is obviously different.S represents laser light scattering, its penetrable cell is surveyed intracellular nucleic leaflet situation and particle situation, analyzes by analyzing light scattering information pair cell endoparticle, the light reflection that the cell endoparticle is thick is strong, therefore can be used for monocyte and three kinds of granulocytic differentiations.By the characteristic of three parameters of overall treatment, can carry out comprehensive evaluation analysis to leukocytic various features comprehensively, obtain five leukocyte differential count results.
2, adopt impedance, laser light scattering and fluorescent dye technology
Wherein direct current impedance method (DC) is used to measure the cell volume size; Forward scattering light, the side scattered light that laser light scattering produces can be used for surveying the situation (nucleus and particle situation) of leucocyte volume size, cell inclusion; Side direction fluorescence then can reacting cells in the content of DNA (deoxyribonucleic acid) (DNA) and RNA (ribonucleic acid) (RNA).Distinctive eosinophil detects special hemolytic agent can be with dissolving of all cells except acidophil or atrophy, and the liquid that contains complete acidophil can be counted according to the electric-resistivity method counting technology by aperture.In the basocyte passage, use special hemolytic agent all cells except basocyte can be dissolved or atrophy, the liquid that contains complete basocyte can be counted according to the impedance method counting technology by aperture, juvenile cell checks that passage (IMI) can contain the less phenomenon of lipid than mature cell film surface according to the juvenile cell film, in the cell dilution suspension, add sulfuration amino acid, because occupy-place difference, it is more to be combined in juvenile cell performance amino acid, hemolytic agent there is resistant function, people's hemolytic agent after ripening cell is easily dissolved when adding, and that juvenile cell is difficult for is destroyed, can detect by electric-resistivity method.Comprehensive each measuring method obtains figure and data that leucocyte five hives off.
3, laser light scattering and cytochemical staining technology
Instrument has adopted the leucocyte peroxidase to measure passage on leukocyte differential count and basophil is measured passage.Feature according to cytochemical staining is carried out leukocyte differential count.All kinds of leucocytes are such to the reaction of peroxidase: early stage myeloblast is negative, and following each stage of myeloblast is all contained peroxidase, and strengthens along with the maturation of cell.Different granulocyte peroxidase content differences, eosinophil has the strongest peroxidase, and neutrophil leucocyte contains stronger peroxidase, and basophil does not contain this enzyme.Monocyte all contains more weak peroxidase except that the early stage primitive stage.Lymphocyte does not contain peroxidase.Measure the information that has also obtained the cell size simultaneously according to the cell volume that the laser method scattered light intensity carries out, on leucocyte peroxidase scatter diagram, X-axis is represented peroxidase intensity, and peroxidase strong positive cell is positioned at right-hand member; Y-axis is represented the scattered light intensity signal, and the expression scattered light signal that is positioned at the top is strong, and cell volume is big.The cell of each type is recorded in certain location according to their characteristics except basocyte, forms classification in the scope of the door of setting.Basocyte is then measured by basocyte and leaflet passage, because of lymph and basocyte all do not have peroxidase, therefore need by the basocyte passage, special basocyte reagent can be with other leucocyte film destroys except that basocyte, endochylema overflows, only surplus bare nucleus.Still kept the basocyte of normal volume to be positioned at the Y-axis top after the laser radiation, other bare nucleus leucocytes that volume is little are distributed in the below.
4, multi-angle polarized light scatter method (MAPSS) technology
The ultimate principle of this technology is that sample enters test section under the effect of hydrodynamic force focusing system, and under the irradiation of laser beam, cell produces scattered light in a plurality of angle portion.Instrument is provided with the signal that four angles are collected scattered light especially.0 degree is used for judging roughly the cell volume size for the anterior angle scattering; 10 degree are the narrow angle scattering, are used to detect the index of eucaryotic cell structure and inner complicacy thereof; 90 degree are used for the analysis of pair cell internal particle and karyolobism situation for the vertical light scattering; 90 degree polarized light scatters can be with the characteristic of the laser depolarization of vertical angle based on particle, acidophic cell is separated from neutrophil leucocyte and other cells, angle and position according to scattered light, four detecting devices of instrument internal can receive corresponding signal, carry out analyzing and processing by the microprocessor of instrument internal.
At present, nearly all full automatic five grouping blood analyser all is to have adopted above-mentioned four kinds of technology on the market.Because these four kinds of technology exist structure very complicated or adopted fluorescent dye or adopted two sheath stream technology or combined this several method, have caused on the present domestic five grouping blood analyser market and have still accounted for leading phenomenon by external instrument.
Summary of the invention
The utility model provides a kind of optical system that is applicable to full automatic five grouping blood analyser, the mode that has proposed DC signal, laser forward scattering signal and laser backscatter signal are combined is as a kind of method of differentiating the leucocyte classification, to overcome problems of the prior art.
The utility model is made up of red laser [R], mirror lens [C], [D], [E], reception lens barrel [M1], [M2], collimation lens barrel [L1], [L2], streaming box [P], photodetector [Q1], [Q2].Place streaming box [P] in the middle of receiving lens barrel [M1], [M2], three's light shaft coaxle, mirror lens [C] are 45 and place the outside that receives lens barrel [M1], and its incident light axis is coaxial with reception lens barrel [M1]; Mirror lens [D], [E] are 45 and place reception lens barrel [M2] outside successively, and both reflection optical axis are coaxial with [M2]; Collimation lens barrel [L1] places on the reflected light axis of mirror lens [C], is thereafter photodetector [Q1]; Collimation lens barrel [L2] places on the reflected light axis of catoptron [D], is thereafter photodetector [Q2]; Red laser [R] places collimation lens barrel [L2] outside, and its optical axis is coaxial with the incident light axis of mirror lens [E], and all assemblies are installed on the base plate [F].
Principle of work of the present utility model is: regulate the adjustment rack of red laser, the whole micropore of ruddiness circular light spot center-aligned center, and can shine whole Ku Erte micropore uniformly.When a leucocyte passes through the Ku Erte micropore,, can produce a DC pulse signal according to Coulter principle.Almost meanwhile, the laser intensity of forward direction irradiation with do not have leucocyte by the time compare a faint variation can take place, be that forward direction irradiating laser intensity can die down relatively, this light scattering signal that dies down is through after receiving lens barrel [M1], after by catoptron [C] scattered signal being injected collimation lens barrel [L1], finally be radiated on the photodetector [Q1], photodetector then produces a faint electric current to be changed, this weak current changes by finally transforming into a pulse behind the amplifying circuit, is called forward light scattering pulse signal.Simultaneously, when leucocyte passes through the Ku Erte micropore, the laser light reflected light intensity with do not have leucocyte by the time compare a faint variation can take place, it is the relative grow of laser light reflected intensity meeting, the light scattering signal of this grow is through after receiving lens barrel [M2], after by catoptron [D] scattered signal being injected collimation lens barrel [L2], finally be radiated on the photodetector [Q2], photodetector then produces a faint electric current to be changed, this weak current changes by finally transforming into a pulse behind the amplifying circuit, is called the backward scattered light pulse signal.Therefore, in conjunction with DC pulse, forward light scattering pulse signal and backward scattered light pulse signal leucocyte being carried out five hives off.
Its adjustment process is: at first optical system by helium-neon laser and mechanical aperture with the beam path alignment of optical system point-blank.Then the Ku Erte micropore in the streaming box, collimation lens barrel and reception lens barrel are encased on the optical system that mixes up light path.Adjust the Ku Erte micropore by adjusting five dimension adjustment racks, make its by receive lens barrel become to amplify 7 times as (forward-backward algorithm all), the articulation point of picture be through the reflection back reflection in the mechanical aperture of collimation lens barrel, promptly the position of mechanical aperture is the focal position of light after receiving lens cone system.Therefore what see on CCD can only be and big Ku Erte micropore picture such as mechanical aperture that the outer picture of Ku Erte micropore is then blocked by mechanical aperture.As becoming the lens face of parallel signal to CCD behind the process collimating mirror cartridge system, lens face is becoming signal gathering focus to show at computer to the CCD light-sensitive surface clearly for this.
The light source of optical system sends light source by laser instrument, enter through catoptron and to receive lens barrel laser is pooled in the Ku Erte micropore that a small light spot shines the streaming box, laser by micropore enters the reception lens barrel, laser by receiving lens barrel is again by mirror reflects, shine the collimation lens barrel, shine photodetector then.
The beneficial effects of the utility model are that streaming box process structure is simple relatively, no sheath flow liquid, no fluorescent dye, do not have other special reagent.
Description of drawings
Fig. 1 is a light path synoptic diagram of the present utility model.Wherein R is a laser instrument; C, D, E are mirror lens; M1, M2 are for receiving barrel assembly; L1, L2 are the collimation barrel assembly; P adjusts assembly for the streaming box; Q1, Q2 are photodetector.
Fig. 2 is the structural drawing of an embodiment of the present utility model.Wherein R is a red laser; C, D, E are mirror lens; M1, M2 are for receiving barrel assembly; L1, L2 are the collimation barrel assembly; P adjusts assembly for the streaming box; Q1, Q2 are photodetector; F is a base plate.
Embodiment
The utility model will be in conjunction with the accompanying drawings, is described further by following examples.
Embodiment.
The utility model is made up of red laser R, mirror lens C, D, E, reception lens barrel M1, M2, collimation lens barrel L1, L2, streaming box P, photodetector Q1, Q2.Place streaming box P in the middle of receiving lens barrel M1, M2, three's light shaft coaxle, mirror lens C are 45 and place the outside that receives lens barrel M1, and its incident light axis is coaxial with reception lens barrel M1; Mirror lens D, E are 45 and place the reception lens barrel M2 outside successively, and both reflection optical axis are coaxial with M2; Collimation lens barrel L1 places on the reflected light axis of mirror lens C, is thereafter photodetector Q1; Collimation lens barrel L2 places on the reflected light axis of catoptron D, is thereafter photodetector Q2; Red laser R places the collimation lens barrel L2 outside, and its optical axis is coaxial with the incident light axis one of mirror lens E, and all assemblies are installed on the base plate F.
It in the streaming box ruby hole (Ku Erte micropore) that a diameter is 0.08mm.
Principle of work of the present utility model is: regulate the adjustment rack of red laser, the whole micropore of ruddiness circular light spot center-aligned center, and can shine whole Ku Erte micropore uniformly.When a leucocyte passes through the Ku Erte micropore,, can produce a DC pulse signal according to Coulter principle.Almost meanwhile, the laser intensity of forward direction irradiation with do not have leucocyte by the time compare a faint variation can take place, be that forward direction irradiating laser intensity can die down relatively, this light scattering signal that dies down is through after receiving lens barrel M1, after by catoptron C scattered signal being injected collimation lens barrel L1, finally be radiated on the photodetector Q1, photodetector then produces a faint electric current to be changed, this weak current changes by finally transforming into a pulse behind the amplifying circuit, is called forward light scattering pulse signal.Simultaneously, when leucocyte passes through the Ku Erte micropore, the laser light reflected light intensity with do not have leucocyte by the time compare a faint variation can take place, it is the relative grow of laser light reflected intensity meeting, the light scattering signal of this grow is through after receiving lens barrel M2, after by catoptron D scattered signal being injected collimation lens barrel L2, finally be radiated on the photodetector Q2, photodetector then produces a faint electric current to be changed, this weak current changes by finally transforming into a pulse behind the amplifying circuit, is called the backward scattered light pulse signal.Therefore, in conjunction with DC pulse, forward light scattering pulse signal and backward scattered light pulse signal leucocyte being carried out five hives off.
The above only is a preferred embodiment of the present utility model; be not so limit patent of the present utility model; every equivalent structure or equivalent flow process conversion that utilizes the utility model instructions and accompanying drawing content to be done; or directly or indirectly be used in other association areas, all in like manner be included in the scope of patent protection of the present utility model.
Claims (1)
1. a full automatic five grouping blood analyser optical system is characterized in that being made up of red laser [R], mirror lens [C], [D], [E], reception lens barrel [M1], [M2], collimation lens barrel [L1], [L2], streaming box [P], photodetector [Q1], [Q2]; Place streaming box [P] in the middle of receiving lens barrel [M1], [M2], three's light shaft coaxle, mirror lens [C] are 45 and place the outside that receives lens barrel [M1], and its incident light axis is coaxial with reception lens barrel [M1]; Mirror lens [D], [E] are 45 and place reception lens barrel [M2] outside successively, and both reflection optical axis are coaxial with [M2]; Collimation lens barrel [L1] places on the reflected light axis of mirror lens [C], is thereafter photodetector [Q1]; Collimation lens barrel [L2] places on the reflected light axis of catoptron [D], is thereafter photodetector [Q2]; Red laser [R] places collimation lens barrel [L2] outside, and its optical axis is coaxial with the incident light axis of mirror lens [E], and all assemblies are installed on the base plate [F].
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010201711987U CN201653904U (en) | 2010-04-26 | 2010-04-26 | Full-automatic optical system for penta-clustering blood analyzer |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010201711987U CN201653904U (en) | 2010-04-26 | 2010-04-26 | Full-automatic optical system for penta-clustering blood analyzer |
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| Publication Number | Publication Date |
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| CN201653904U true CN201653904U (en) | 2010-11-24 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010201711987U Expired - Lifetime CN201653904U (en) | 2010-04-26 | 2010-04-26 | Full-automatic optical system for penta-clustering blood analyzer |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101819145A (en) * | 2010-04-26 | 2010-09-01 | 南昌百特生物高新技术股份有限公司 | Full automatic five grouping blood analyser optical system |
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2010
- 2010-04-26 CN CN2010201711987U patent/CN201653904U/en not_active Expired - Lifetime
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101819145A (en) * | 2010-04-26 | 2010-09-01 | 南昌百特生物高新技术股份有限公司 | Full automatic five grouping blood analyser optical system |
| CN101819145B (en) * | 2010-04-26 | 2011-09-14 | 南昌百特生物高新技术股份有限公司 | Full automatic five grouping blood analyser optical system |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term |
Granted publication date: 20101124 |
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| CX01 | Expiry of patent term |