CN201648394U - Light illuminator for cell photodynamic curative effect assessment - Google Patents
Light illuminator for cell photodynamic curative effect assessment Download PDFInfo
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- CN201648394U CN201648394U CN2009201834804U CN200920183480U CN201648394U CN 201648394 U CN201648394 U CN 201648394U CN 2009201834804 U CN2009201834804 U CN 2009201834804U CN 200920183480 U CN200920183480 U CN 200920183480U CN 201648394 U CN201648394 U CN 201648394U
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- led array
- plate
- tissue culture
- culture plate
- cell
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Abstract
The utility model relates to a light illuminator for cell photodynamic curative effect assessment, which belongs to a biomedicine instrument. The light illuminator consists of a box body, an LED array light source lamp plate group, a light decay sheet array support plate group, a cell culture plate fixing groove, a cell culture plate and a circuit part, wherein grooves are arranged on the left inner side surface and the right inner side surface of the box body, the LED array light source lamp plate group consists of a plurality of LED array light source lamp plates, the light decay sheet array support plate group consists of a plurality of support plates, sheet supporters of the light decay sheet are arranged on each support plate, the cell culture plate is positioned right under the light decay sheet array support plates, a positioned bulge fixing frame is arranged under the cell culture plate, hollow cylindrical concave base holes are also arranged on the cell culture plate, and the circuit part is arranged in a circuit box of a base of a reference instrument box body, and is provided with a temperature controller, an electrification timing device and a work switch. The utility model can enlarge the light illumination area, can simplify the operation program, can reduce the light source cost, and can be applied to isolated cell photodynamic action effect research.
Description
Technical field
The utility model relates to a kind of illumination apparatus that is used for the assessment of cell light power curative effect, relate to a kind of photodynamic therapy (Photodynamic Therapy that can be used for specifically, PDT) in, the device that utilizes the led array light source that the viable cell rayed that exsomatizes is cultivated belongs to biomedical quasi-instrument.
Background technology
PDT utilizes light, photosensitizers and oxygen molecule a kind of the associating, optionally treats tumour by photodynamic reaction, and the new therapy of benign diseases such as age-related macular degeneration and nevus flammeus.For a long time, light source is as an important component part of carrying out cell PDT experimental study, and what adopt usually is output different wavelength of laser device.Tissue Culture Plate with six holes is an example, and six orifice plates are of a size of 123 * 83mm
2, in the isolated cells experimental study, common way is to adopt laser beam expanding system that laser is collimated to expand bundle, can both receive the irradiation of even laser to guarantee all cells.Owing to be subjected to the restriction of laser beam expanding area, expand the bundle hot spot and can't cover 123 * 83mm at all
2Irradiated area.If when the cell in six holes experimentizes comparative study on the pair cell culture plate, then must collimate and expand bundle, afterwards single hole is shone one by one the tiny light beam of laser apparatus output.Simultaneously, laser beam expanding need design special optical system, also has operation stepss such as light path adjustment during use.This not only brings inconvenience to experiment, and when carrying out control experiment research, is difficult to guarantee that the cell in each hole has identical growing environment when accepting laser irradiation.
Along with the continuous maturation of LED manufacturing technology, LED constantly highlights as the advantage of radiation source.LED has long, stable height of life-span, brightness is high and emission wavelength wide coverage lamp advantage, and is simultaneously cheap.Therefore, utilize led light source to substitute conventional light source, not only can guarantee the illumination quality, and can also greatly reduce the irradiation cost, be applied to shine or the cost performance in field such as illumination thereby improved it effectively.
Summary of the invention
At traditional isolated cells light power effect research, with laser deficiencies such as the bundle of expansion area is limited, operation inconvenience are arranged as radiation source, the purpose of this utility model just provide a kind of with led array as light source, design and be used for the cell rayed culture apparatus that isolated cells light power research is used.Can improve illuminating area effectively by this device, the degree that simplifies the operation has greatly reduced the light source irradiation cost.
For realizing that the technical scheme that the purpose of this utility model adopts is: cell rayed culture apparatus mainly is made of casing, led array light source lamp plate group, optical attenuation chip arrays supporting plate group, Tissue Culture Plate pickup groove, Tissue Culture Plate and circuit part.Casing is an inside and outside double walled hollow cubic body structure, and positive side is provided with the monolateral door of opening of a fan; Casing left and right sides medial surface is provided with two pairs of grooves that the parallel left and right sides is contour; The backboard medial surface of casing and first pair of groove center line equal-height position are provided with a supply socket; The base of casing is provided with circuit box.
Led array light source lamp plate group is by a plurality of equal and opposite in directions, the led array lamp plate of LED emission wavelength difference but the uniform rectangular plate shape structure of illumination is formed, on each led array lamp plate fixed wave length is installed, light-emitting area down, the led array that is evenly distributed, the limit, the left and right sides of led array lamp plate just in time embeds in first pair of groove, the back side edge of led array lamp plate is provided with an attaching plug simultaneously, when the led array lamp plate embeds in first pair of groove, attaching plug also just in time inserts and is arranged in the backboard medial surface supply socket of casing, to guarantee the power supply on the socket LED on the led array lamp plate is powered.
Optical attenuation chip arrays supporting plate group is made up of a plurality of supporting plates, the optical attenuation sheet sheet holder that each supporting plate is provided with equal and opposite in direction, is evenly distributed, the sheet holder is that two hollow concentric(al) circless constitute up and down, goes up hollow circular diameter and is a bit larger tham optical attenuation sheet diameter, and following open circles diameter is less than optical attenuation sheet diameter; Supporting plate is divided into 6 orifice plates, 12 orifice plates, 24 orifice plates, 48 orifice plates, 96 orifice plates and 384 orifice plates according to the quantity of sheet holder; Limit, the supporting plate left and right sides just in time embeds in second pair of groove.When carrying out cell PDT light dosage control experiment research, can in the sheet holder, place different optical attenuation sheets, to change the illumination power density that different datum hole cells are received on the Tissue Culture Plate according to the needs of light intensity.
Tissue Culture Plate is positioned under the optical attenuation chip arrays supporting plate.What the below of Tissue Culture Plate correspondence was provided with in-plane is used for the localized projection fixed frame of Tissue Culture Plate, and the Tissue Culture Plate bottom surface is just in time wherein chimeric.By directly Tissue Culture Plate being put into Tissue Culture Plate fixed frame, replacing and the location that just can realize Tissue Culture Plate.
The hollow cylindrical matrix datum hole that Tissue Culture Plate is provided with equal and opposite in direction, is evenly distributed.Tissue Culture Plate is divided into 6 orifice plates, 12 orifice plates, 24 orifice plates, 48 orifice plates, 96 orifice plates and 384 orifice plates by the quantity of datum hole.Principle according to the hole count correspondence, the corresponding supporting use of 6 orifice plates of 6 orifice plates of Tissue Culture Plate and optical attenuation chip arrays supporting plate during use, by that analogy, so the central position that guarantees each sheet holder be with the central position of each datum hole of Tissue Culture Plate that axis is corresponding up and down.
Be arranged in the circuit box of cell rayed culture apparatus cabinet base, be provided with temperature controller, energising timing device and operating switch.When cell rayed culture apparatus is used for the isolated cells cultivation, can make the indoor homo(io)thermism of cell cultures in 37 ℃, be beneficial to cell cultures by temperature controller.Device when the energising timing device is certain, according to requirement of experiment, irradiation time can be adjusted between 600 minutes at 1 minute.After external source enters cell rayed culture apparatus, in parallel, link to each other with operating switch with the supply socket of backboard medial surface, temperature controller, the energising timing device that are arranged on casing respectively by lead.
The beneficial effects of the utility model are, replaced laser apparatus as the light source of carrying out cell PDT experimental study with the led array light source, expanded illuminating area effectively, whole experimental system is integrated, greatly reduce the cost of radiation source and the fussy degree of isolated cells PDT experimental implementation, and improve the accuracy of control experiment.
Description of drawings
Below with reference to accompanying drawing utility model is described in further detail.
Fig. 1 is the casing perspective view of the utility model when not adding lamp plate, optical attenuation sheet placing plate and Tissue Culture Plate.
Fig. 2 is a cell rayed culture apparatus front view described in the utility model.
Fig. 3 is the LED distribution schematic diagram of led array light source lamp plate surface of light source.
Fig. 4 is the circuit distribution plan of led array light source lamp plate circuit face.
Fig. 5 is not adding optical attenuation sheet time attenuator array supporting plate longitudinal cross-section sectional view.
Fig. 6 is Tissue Culture Plate and is used for the localized projection fixed frame of Tissue Culture Plate longitudinal cross-section sectional view.
Fig. 7 is led array light source lamp plate and casing slot and outlet docking scheme.
Fig. 8 is the complete Circuits System connection diagram of the utility model.
Fig. 9 is the light distribution that arrives Tissue Culture Plate when not adding the optical attenuation sheet.
Figure 10 is the light distribution that arrives Tissue Culture Plate when placing 10%, 20%, 30%, 40% and 50% 5 optical attenuation sheet.
Among Fig. 1,1 is cell rayed culture apparatus cabinet door, which is provided with glass window, 2 is optical attenuation chip arrays supporting plate slot, and 3 is led array light source lamp plate slot, and 4 is led array light source lamp plate outlet, 5 is casing, 6 is the casing glass window, and 7 is the timer display panels, and 8 is the timer push button, 9 is the temperature regulator display panels, 10 is the Tissue Culture Plate pickup groove, and 11 is the temperature regulator push button, and 12 is total power switch.
Among Fig. 2,1 is cell rayed culture apparatus cabinet door, which is provided with glass window, 2 is optical attenuation chip arrays supporting plate slot, 3 is led array light source lamp plate slot, 5 is casing, and 6 is the casing glass window, and 7 is the timer display panels, 8 is the timer push button, 9 is the temperature regulator display panels, and 10 is the Tissue Culture Plate pickup groove, and 11 is the temperature regulator push button, 12 is total power switch, 13 is led array light source lamp plate, and 15 is optical attenuation sheet placing plate, and 16 is Tissue Culture Plate.
Among Fig. 3,13 is led array light source lamp plate, and 14 is the LED lamp.
Among Fig. 4,13 is led array light source lamp plate attaching plug., 17 for the LED lamp is electrically connected circuit, and 18 is led array light source lamp plate
Among Fig. 5,13 is led array light source lamp plate, and 19 are the equal and opposite in direction that is provided with on the optical attenuation chip arrays supporting plate, the optical attenuation sheet sheet holder that is evenly distributed.
Among Fig. 6,10 is the Tissue Culture Plate pickup groove, and 16 is Tissue Culture Plate.
Among Fig. 7,3 is led array light source lamp plate slot, and 4 is led array light source lamp plate outlet, and 13 is led array light source lamp plate, and 18 is led array light source lamp plate attaching plug.
Among Fig. 8,4 is led array light source lamp plate outlet, and 12 is total power switch, and 20 is temperature regulator, and 21 is electric wire, and 22 is the general supply plug, and 23 is timer.
Among Fig. 9, the ordinate zou of left figure is represented the lengthwise position coordinate, and X-coordinate is represented the horizontal position coordinate, and the dark part in middle part is represented light intensity value among the figure, the different different light intensities of gray scale representative.Right figure is along the light distribution of level and vertical direction at cross wire among the left figure.
Among Figure 10, the ordinate zou of left figure is represented the lengthwise position coordinate, and X-coordinate is represented the horizontal position coordinate, and the dark part in middle part is represented light intensity value among the figure, the different different light intensities of gray scale representative.Right figure is along the light distribution of level and vertical direction at cross wire among the left figure.
Embodiment
Embodiment 1
Described according to Fig. 1～Fig. 8 part, structure is based on the cell rayed culture apparatus of led array, and wherein: the length of casing (5) is respectively 300mm, 220mm, 550mm; Timer (23) adopts Coud TW-L12 electronic timer; Temperature regulator (20) adopts WK-8000 type multi-functional temp controller, and the Controllable Temperature scope is-50 ℃ to 150 ℃; It is the Tissue Culture Plate (16) of 24 (4 * 6 distribute) that Tissue Culture Plate (16) adopts the quantity of datum hole, be positioned over that the cell in the datum hole is nasopharyngeal carcinoma cell C666-1 in the Tissue Culture Plate (16), cell is carried out the PDT action effect and is contrasted experimental study after hatching 3 hours altogether with photosensitizers (good fortune contest because of); It is 24 orifice plates that optical attenuation chip arrays supporting plate (15) adopts sheet holder (19) quantity, is equipped with transmitance and is respectively 4 groups every group each optical attenuation sheet of 6 of 10%, 20%, 30% and 40%; Choosing the peak wavelength that is complementary because of optimum absorb wavelength with the good fortune contest is the led array light source lamp plate (13) of 660nm, and led array light source lamp plate (13) one side is at 115.83 * 77.22mm
2Scope in, the LED lamp (14) that 70 peak wavelengths of uniform distribution are 660nm, phase mutual edge distance between the LED lamp (14) is 12.87mm, another side at led array light source lamp plate (13), it is the electrical connection circuit (17) that LED lamp (14) is linked together, being electrically connected circuit (17) mainly is made up of the driving circuit and the series-parallel junction circuit of LED lamp of LED lamp, can guarantee that each lamp works under rated current 350mA, as long as, just can power for each LED lamp (14) of whole lamp plate with led array light source lamp plate (13) outlet (4) of LED lamp plate attaching plug (18) insertion casing (1).Tissue Culture Plate (16) can be satisfied at the 123 * 83mm of this place in the position of Tissue Culture Plate (16) and led array light source lamp plate light-emitting area vertical range 100mm
2Areal extent in light intensity unevenness≤± 1%, meet the illumination requirement of PDT research.
General supply plug (22) is plugged in, press total power switch (12), system begins power supply.We can know from Fig. 8, temperature regulator (20) directly inserts the general supply line, and LED lamp plate outlet (4) front end also is connected to a timer (23), if when current to being 10:00,10:05 will be made as conduction time of timer (23) by timer push button (8), power-off time is made as 10:35, carries out 30 minutes rayed.By temperature regulator push button (11) temperature of temperature regulator (20) is set in 37 ℃.Shut cabinet door (1), we can see through the reading that casing glass window (6) is observed temperature regulator display panels (9) and timer display panels (7).Cell rayed culture apparatus will be switched in the time of 10:05 and be made the LED lamp (14) in the led array light source lamp plate (13) luminous, LED lamp (14) beginning pair cell liquid shines, automatically cut off the power supply during 10:35, LED lamp (14) in the led array light source lamp plate (13) stops luminous, and experiment finishes.We can open cabinet door (1), after extracting optical attenuation sheet placing plate (15), just can take out Tissue Culture Plate (16), observe the survival condition of cell under different illumination conditions in 24 holes respectively, thereby carry out the quantitative comparison of PDT action effect with microscope.
Embodiment 2
Timer (23), temperature regulator (20) adopt the product of embodiment 1; It is the Tissue Culture Plate (16) of 6 (2 * 3 distribute) that Tissue Culture Plate (16) adopts the quantity of datum hole, the cell that is positioned in the middle datum hole of Tissue Culture Plate (16) is nasopharyngeal carcinoma cell CNE1, and cell is carried out the PDT action effect and contrasted experimental study after hatching 24 hours altogether with photosensitizers (the happiness pool divides); It is 6 orifice plates that optical attenuation chip arrays supporting plate (15) adopts sheet holder (19) quantity, is equipped with transmitance and is respectively 10%, 20%, 30%, 40% and 50% optical attenuation sheet; Choosing and like the peak wavelength that pool divides optimum absorb wavelength to be complementary is the led array light source lamp plate (13) of 627nm, and led array light source lamp plate (13) one side is at 117 * 91mm
2Scope in, the LED lamp (14) that 63 peak wavelengths of uniform distribution are 627nm, phase mutual edge distance between the LED lamp (14) is 13mm, another side at led array light source lamp plate (13), it is the electrical connection circuit (17) that LED lamp (14) is linked together, being electrically connected circuit (17) mainly is made up of the driving circuit and the series-parallel junction circuit of LED lamp of LED lamp, can guarantee that each lamp works under rated current 350mA, as long as, just can power for each LED lamp (14) of whole lamp plate with led array light source lamp plate (13) outlet (4) of LED lamp plate attaching plug (18) insertion casing (1).Tissue Culture Plate (16) can be satisfied at the 123 * 83mm of this place in the position of Tissue Culture Plate (16) and led array light source lamp plate light-emitting area vertical range 100mm
2Areal extent in light intensity unevenness≤± 1%, meet the illumination requirement of PDT research.
Operating process is with embodiment 1.
Timer (23), temperature regulator (20) adopt the product of embodiment 1; The quantity of Tissue Culture Plate (16) employing datum hole is 96 Tissue Culture Plate (16), the cell that is positioned in the middle datum hole of Tissue Culture Plate (16) is leukemia cell HL60, and cell is carried out the photodynamic action effect according to experimental study after hatching 4 hours altogether with photosensitizers (rose-red); It is 96 (6 * 16 distribute) orifice plate that optical attenuation chip arrays supporting plate (15) adopts sheet holder (19) quantity, is equipped with transmitance and is respectively 6 groups every group each optical attenuation sheet of 16 of 10%, 20%, 30%, 40% and 50%; Choosing the peak wavelength that is complementary with rose-red optimum absorb wavelength is the led array light source lamp plate (13) of 530nm, and led array light source lamp plate (13) one side is at 120 * 80mm
2Scope in, the LED lamp (14) that 96 peak wavelengths of uniform distribution are 530nm, phase mutual edge distance between the LED lamp (14) is 10mm, another side at led array light source lamp plate (13), it is the electrical connection circuit (17) that LED lamp (14) is linked together, being electrically connected circuit (17) mainly is made up of the driving circuit and the series-parallel junction circuit of LED lamp of LED lamp, can guarantee that each lamp works under rated current 350mA, as long as, just can power for each LED lamp (14) of whole lamp plate with led array light source lamp plate (13) outlet (4) of LED lamp plate attaching plug (18) insertion casing (1).Tissue Culture Plate (16) can be satisfied at the 123 * 83mm of this place in the position of Tissue Culture Plate (16) and led array light source lamp plate light-emitting area vertical range 100mm
2Areal extent in light intensity unevenness≤± 1%, meet the illumination requirement of PDT research.
Claims (7)
1. an illumination apparatus that is used for the assessment of cell light power curative effect is characterized in that this device is made of casing, led array light source lamp plate group, optical attenuation chip arrays supporting plate group, Tissue Culture Plate pickup groove, Tissue Culture Plate and circuit part, wherein:
Described casing is an inside and outside double walled hollow cubic body structure, and casing left and right sides medial surface is provided with two pairs of grooves that the parallel left and right sides is contour;
Described led array light source lamp plate group is made up of a plurality of led array lamp plates, and the limit, the left and right sides of led array lamp plate just in time embeds on the medial surface of the casing left and right sides in first pair of groove;
Described optical attenuation chip arrays supporting plate group is made up of a plurality of supporting plates, and each supporting plate is provided with the holder of optical attenuation sheet sheet, and limit, the supporting plate left and right sides just in time embeds on the medial surface of the casing left and right sides in second pair of groove;
Described Tissue Culture Plate is positioned under the optical attenuation chip arrays supporting plate, and the Tissue Culture Plate below is provided with localized projection fixed frame, also is provided with hollow cylindrical matrix datum hole on the Tissue Culture Plate;
Described circuit part is arranged in the circuit box of cell rayed culture apparatus cabinet base, is provided with temperature controller, energising timing device and operating switch.
2. a kind of illumination apparatus that is used for the assessment of cell light power curative effect according to claim 1, it is characterized in that the positive side of described casing also is provided with the monolateral door of opening of a fan, the backboard medial surface of casing and first pair of groove center line equal-height position are provided with a supply socket.
3. a kind of illumination apparatus that is used for the assessment of cell light power curative effect according to claim 1, it is characterized in that being equipped with on the described led array lamp plate that wavelength is identical, light-emitting area down, the led array that is evenly distributed, the back side edge of led array lamp plate is provided with an attaching plug simultaneously.
4. a kind of illumination apparatus that is used for the assessment of cell light power curative effect according to claim 1, it is characterized in that the sheet holder that is provided with on the described optical attenuation chip arrays supporting plate is that two hollow concentric(al) circless constitute up and down, go up hollow circular diameter and be a bit larger tham optical attenuation sheet diameter, following open circles diameter is less than optical attenuation sheet diameter, the quantity of sheet holder equates with the datum hole quantity of Tissue Culture Plate in the supporting plate, and the position of sheet holder and the position of the datum hole of Tissue Culture Plate are that axis is corresponding up and down.
5. a kind of illumination apparatus that is used for the assessment of cell light power curative effect according to claim 4 is characterized in that described optical attenuation chip arrays supporting plate is divided into 6 orifice plates, 12 orifice plates, 24 orifice plates, 48 orifice plates, 96 orifice plates and 384 orifice plates according to the quantity of sheet holder.
6. a kind of illumination apparatus that is used for the assessment of cell light power curative effect according to claim 1 is characterized in that the quantity of described Tissue Culture Plate datum hole is divided into 6 holes, 12 holes, 24 holes, 48 holes, 96 holes and 384 holes.
7. a kind of illumination apparatus that is used for the assessment of cell light power curative effect according to claim 1, it is characterized in that described circuit part is after external source enters cell rayed culture apparatus, in parallel, link to each other with operating switch with the supply socket of backboard medial surface, temperature controller, the energising timing device that are arranged on casing respectively by lead.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009201834804U CN201648394U (en) | 2009-10-28 | 2009-10-28 | Light illuminator for cell photodynamic curative effect assessment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009201834804U CN201648394U (en) | 2009-10-28 | 2009-10-28 | Light illuminator for cell photodynamic curative effect assessment |
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| Publication Number | Publication Date |
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| CN201648394U true CN201648394U (en) | 2010-11-24 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN2009201834804U Expired - Lifetime CN201648394U (en) | 2009-10-28 | 2009-10-28 | Light illuminator for cell photodynamic curative effect assessment |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101701183B (en) * | 2009-10-28 | 2012-07-18 | 福建师范大学 | Isolated cell photodynamic action effect reference apparatus based on LED array |
| CN103173355A (en) * | 2013-04-18 | 2013-06-26 | 中国医学科学院生物医学工程研究所 | Cell culture incubator with low-level laser environment |
| CN106414705A (en) * | 2014-05-14 | 2017-02-15 | 奥林巴斯株式会社 | Culture observation apparatus |
| CN112831418A (en) * | 2021-04-07 | 2021-05-25 | 广州市丹树生物科技有限责任公司 | In-vitro high-flux screening device for phototherapy and application thereof |
-
2009
- 2009-10-28 CN CN2009201834804U patent/CN201648394U/en not_active Expired - Lifetime
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101701183B (en) * | 2009-10-28 | 2012-07-18 | 福建师范大学 | Isolated cell photodynamic action effect reference apparatus based on LED array |
| CN103173355A (en) * | 2013-04-18 | 2013-06-26 | 中国医学科学院生物医学工程研究所 | Cell culture incubator with low-level laser environment |
| CN106414705A (en) * | 2014-05-14 | 2017-02-15 | 奥林巴斯株式会社 | Culture observation apparatus |
| CN112831418A (en) * | 2021-04-07 | 2021-05-25 | 广州市丹树生物科技有限责任公司 | In-vitro high-flux screening device for phototherapy and application thereof |
| CN112831418B (en) * | 2021-04-07 | 2024-04-23 | 广州市丹树生物科技有限责任公司 | In-vitro high-throughput screening device for phototherapy and application thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20101124 Effective date of abandoning: 20091028 |