CN201522476U - Electrophoresis tank with cooling chamber - Google Patents
Electrophoresis tank with cooling chamber Download PDFInfo
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- CN201522476U CN201522476U CN2009202580129U CN200920258012U CN201522476U CN 201522476 U CN201522476 U CN 201522476U CN 2009202580129 U CN2009202580129 U CN 2009202580129U CN 200920258012 U CN200920258012 U CN 200920258012U CN 201522476 U CN201522476 U CN 201522476U
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- central frame
- groove
- glass plate
- bottom land
- tank
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Abstract
An electrophoresis tank with a cooling chamber is an experimental facility of biochemistry and molecular biology, and comprises an upper tank main body and a lower tank base, wherein the upper tank main body which can be a double-sided rubber chamber or single-sided rubber chamber structure comprises a central frame, a U-shaped notch glass plate, a rectangular glass plate, a fastening press plate, a screw knob and the like; the central frame and plates at two sides thereof are combined so as to form a cooling liquid cavity, a buffer liquid upper tank and the like; and the lower tank base is a rectangular shallow tank, and the opening dimension of a tank body is matched with that of the upper tank main body so as to lead the upper tank main body to be rightly placed therein. The electrophoresis tank is provided with a temperature control circulation cooling liquid cavity between the rubber chamber and the glass plate, improves the speed and the quality of electrophoresis; after silanization treatment, the surface of the U-shaped notch glass plate achieves hydrophobic property, thus leading the stripping of the rubber plates to be faster; and the electrophoresis tank eliminates the leakage of the buffer liquid, reduces the equipment investment and experimental cost, is especially convenient and simple in assembly and disassembly operations when in use.
Description
Affiliated technical field
The utility model relates to a kind of electrophoresis tank that has cooling chamber, belongs to biological chemistry and molecular biology experiment equipment.Be used for albumen, nucleic acid isogel electrophoresis, be particularly useful for more albumen of specimen or nucleic acid gel electrophoresis, as seed purity detection, medical science detection etc.
Background technology
Electrophoresis tank is the usual means in the Biochemistry Experiment, and is also different according to different its project organizations of purposes.The electrophoresis tank that wherein is usually used in purposes such as protein isolate, nucleic acid sequencing mostly is vertical board-like gel electrophoresis groove.Existing various vertical slab gel electrophoresis groove can be divided into single gel slab and two types of two gel slabs, and the mounting means of glue chamber glass plate has sandwich and side-mounted, and the type of cooling has air natural cooling, damping fluid cooling, the metallic heat radiating plate type of cooling.
Adopt the electrophoresis tank radiating effect of air natural cooling the poorest,, therefore seldom adopt at present improving electrophoretic velocity and guaranteeing that the electrophoresis quality is totally unfavorable.
Another kind of cooling method is at side glue chamber glass plate outside applying a slice metallic heat radiating plate, the drawback of this mode is to make the structure complicated of device and increased weight, in addition owing to heat-conducting medium is filled in inconvenience between metallic heat radiating plate and glass, cause the bad conduction or the inhomogeneous conduction of heat, influence electrophoretic separation effect and result's reappearance.
Cool-down method commonly used at present is the damping fluid cooled, promptly in groove, pour into a large amount of electrode buffers, damping fluid directly contacts with glue chamber glass plate, this method can effectively be controlled temperature and obtain electrophoresis result preferably, but need a large amount of damping fluid of additive decrementation, therefore increased experimental cost.In order further to improve cooling-down effect, another scheme is that cooling coil is placed in the damping fluid, passes to liquid coolant in the cooling coil, and this method need still need to carry out heat exchange by damping fluid, causes the waste of damping fluid equally, and troublesome poeration.
Summary of the invention
For overcoming some deficiency or drawback in the above-mentioned prior art, the utility model provides a kind of electrophoresis tank that has cooling chamber, this electrophoresis tank adopts vertical glue chamber glass-plate structure, double faced adhesive tape chamber (respectively there is one group of glue chamber glass plate both sides) or single face glue chamber (side is one group of glue chamber glass plate, and opposite side is a blind plate) structure can be arranged.The cooling sap cavity is set between plate, and cooling sap cavity and buffering liquid groove are isolated, can inject liquid coolant, by the heat interchange of liquid coolant and glue chamber glass, can control the temperature of offset plate in the electrophoresis process, help improving electrophoretic velocity and quality.
For achieving the above object, the utility model is taked following technical scheme: a kind of electrophoresis tank that has cooling chamber is made of last groove body and following bottom land seat two parts.Last groove body is made of the bonding glue-line of central frame and both sides thereof, U type breach glass plate, rectangular glass, clam member, screw rod knob etc.Aforementioned central frame both upper ends thereof is provided with the groove binding post respectively and advances/water delivering orifice, each is bonding or inlay the glass plate that has U type breach that handle through silanization on a surface in the central frame both sides, and its U type breach matches with the U type mouth on top, central frame side; Perhaps, make the framed structure sealing of this side, form single glue chamber glass-plate structure at rectangle organic glass blind plate that physical dimension is identical of aforementioned central frame one side bonding.Behind the bonding U type breach glass plate, the central frame Lower Half forms the cavity that can inject liquid coolant, the advancing of this cavity and central frame end/water delivering orifice communicates, it is 0.2-2mm that two edges, the aforementioned U type breach glass plate outside respectively are provided with thickness, width is the bar shaped pad of 1-4mm, in its outside stack and the identical rectangular glass of aforementioned U type breach glass plate physical dimension, the bar shaped pad at its edge makes and forms penetrating encapsulating crack up and down between two glass plates, but respectively be provided with the clam member that a horizontal slip also can be spacing at the two ends of central frame two sides, the clamping rectangular glass edge but clam member inwardly slides, and, make rectangular glass fix and realize sealing at bar shaped pad place by clamping clam member through the screw rod knob of clam member.The U type breach of U type breach glass plate is for pouring into Signa Gel design, the rectangular glass in encapsulating rear center's framework upper and its outside promptly forms a groove of opening above simultaneously, this groove depth is 10-40mm, bottom land axis distribution one row's platinum filament fixed pivot, the platinum filament suspension thereon, apart from bottom land 1-2mm, gel trickling platinum filament is capped and influences conduction when preventing encapsulating, one end of platinum filament is drawn by the central frame end, is connected in the last groove binding post that is arranged on this end.Following bottom land seat is a rectangle shallow slot, and groove depth is 20-60mm, and the cell body opening size cooperates with last groove body, groove body is inserted wherein just, axis, bottom land surface stationkeeping has platinum filament, and platinum filament one end is drawn by following bottom land seat end, is connected in the following groove binding post that is arranged on this end.
The utility model designs at some deficiency or drawback in the prior art, its beneficial effect is, owing to temperature control circulating cooling liquid cavity is set in central frame, by the heat interchange of liquid coolant and glue chamber glass, the temperature of offset plate in the electrophoresis process be can control, electrophoretic velocity and quality improved; The compact simple volume of the utility model project organization is little, can stop the damping fluid seepage, increase each quantity of handling sample, reduce equipment investment and experimental cost, for example, the consumption of damping fluid reduces to 250ml/ offset plate (or 500ml/ offset plate) from the 700ml/ offset plate of existing apparatus; Because handling through silanization, U type breach glass pane surface is hydrophobic state, so offset plate breaks away from smooth unusually from its surface; The design of each functional unit sufficient humanization, when this electrophoresis tank used, dismounting, operation were particularly convenient.
Description of drawings
Fig. 1 is the utility model one-piece construction synoptic diagram (a double faced adhesive tape cell structure embodiment).Fig. 2 is the exploded view (double faced adhesive tape embodiment) of last groove body.Fig. 3 is the utility model one-piece construction synoptic diagram (a single face glue chamber embodiment).Fig. 4 is last groove body and one side component cut-open view (single face glue cell structure embodiment).Fig. 5 is following bottom land seat synoptic diagram.
Among the figure, 1-central frame, the bonding glue-line of 2-, 3-U type breach glass plate, 4-bar shaped pad, 5-rectangular glass, the 6-clam member, 7-screw rod knob, the last groove binding post of 8-, 9-advances/water delivering orifice, 10-platinum filament fixed pivot, the last groove platinum filament of 11-, bottom land seat under the 12-, groove binding post under the 13-, groove platinum filament under the 14-, 15-rectangle organic glass blind plate.
Concrete embodiment
As Fig. 1, Fig. 2, Fig. 3, Fig. 4 and shown in Figure 5, the utility model is made of last groove body and following bottom land seat two parts, makes by insulating material except that electrode and binding post.Adopt the vertical glue of single or double chamber glass-plate structure, so two kinds of embodiments are arranged:
Embodiment 1:
Two-sided vertical glue chamber glass-plate structure.Last groove body is by bonding glue-line (2), U type breach glass plate (3), bar shaped pad (4), rectangular glass (5), clam member (6), the screw rod knob formations such as (7) of central frame (1) and both sides thereof.Central frame (1) both upper ends thereof is provided with groove binding post (8) respectively and advances/water delivering orifice (9), at central frame (1) two side bonds or inlay the U type breach glass plate (3) that handle through silanization on a surface, its U type breach matches with the U type mouth of central frame (1) two upper lateral part, behind the bonding U type breach glass plate (3), the sealing of central frame (1) Lower Half forms the cavity that can inject liquid coolant, the advancing of this cavity and central frame (1) top one end/water delivering orifice (9) communicates, it is 0.2-2mm that U type breach glass plate (3) outside edges at two ends respectively is fixed with thickness, width is the bar shaped pad (4) of 1-4mm, rectangular glass (5) is superimposed upon U type breach glass plate (3) outside, rectangular glass (5) is identical with U type breach glass plate (3) physical dimension, the bar shaped pad (4) at its edge makes and forms penetrating encapsulating crack up and down between two glass plates, at central frame (1) but the two ends of two sides respectively are provided with a horizontal slip and clam member (6) that can be spacing, clam member (6) but clamping rectangular glass (5) edge that inwardly slides, clamp clam member (6) by screw rod knob (7), rectangular glass (5) is fixed and locate realization to seal at bar shaped pad (4) through clam member (6).From the U type indentation, there of U type breach glass plate (3) encapsulating and place sample comb easily, the rectangular glass (5) in encapsulating rear center framework (1) top and its outside promptly forms a groove of opening above, this groove depth is 10-40mm, bottom land axis distribution one row's platinum filament fixed pivot (10), last groove platinum filament (11) suspension is on it, apart from bottom land 1-2mm, the gel trickling is gone up groove platinum filament (11) and is capped and influences conduction when preventing encapsulating, one end of last groove platinum filament (11) is drawn by central frame (1) end, is connected in the last groove binding post (8) that is arranged on this end.Following bottom land seat (12) is a rectangle shallow slot, groove depth is 20-60mm, the cell body opening size cooperates with last groove body, groove body is inserted wherein just, axis, bottom land surface stationkeeping has following groove platinum filament (14), one end of following groove platinum filament (14) is drawn by following bottom land seat (12) end, is connected in the following groove binding post (13) that is arranged on this end.
Embodiment 2:
The vertical glue of single face chamber glass-plate structure.As shown in Figure 3, Figure 4, this structure difference from Example 1 is, a side bonds rectangle organic glass blind plate (15) of described central frame (1) substitutes a series of superimposed layers of this side, and opposite side is identical with embodiment 1, formation single face glue cell structure.
Dismounting when this electrophoresis tank uses, operation are very convenient, two rectangular glass (5) need only be overlapped the outside of U type breach glass plate (3), and clam member (6) is inwardly slided, and screw screw rod knob (7), can make offset plate.After offset plate solidifies, add testing sample,, will go up the groove body seat and go into bottom land seat (12) down, in cooling chamber, inject liquid coolant, can power up the enforcement electrophoresis at upper and lower groove injecting electrode damping fluid.Electrophoresis process finishes, groove damping fluid and liquid coolant in the discharge, to go up groove body from taking out the bottom land seat (12) down, unscrew screw rod knob (7), the clam member (6) that outwards slides, hand-held then rectangular glass (5) upper limb, light and slow ground is outwards peeled off, be hydrophobic state because handle through silanization on U type breach glass plate (3) surface, so offset plate can strip down down-streams such as the development of can dyeing then smoothly with rectangular glass (5).
Claims (5)
1. electrophoresis tank that has cooling chamber, constitute by last groove body and following bottom land seat two parts, it is characterized in that, last groove body comprises the bonding glue-line (2) of central frame (1) and both sides thereof, U type breach glass plate (3), bar shaped pad (4), rectangular glass (5), clam member (6), screw rod knob (7), central frame (1) both upper ends thereof is provided with groove binding post (8) respectively and advances/water delivering orifice (9), each is bonding or inlay a U type breach glass plate (3) in central frame (1) both sides, behind the bonding U type breach glass plate (3), the sealing of central frame (1) Lower Half forms the cavity that can inject liquid coolant, the advancing of this cavity and central frame (1) end/water delivering orifice (9) communicates, it is 0.2-2mm that U type breach glass plate (3) outside edges at two ends is provided with thickness, width is the bar shaped pad (4) of 1-4mm, rectangular glass (5) is superimposed upon U type breach glass plate (3) outside, central frame (1) but the two ends of two sides respectively are provided with a horizontal slip and clam member (6) that can be spacing and through the screw rod knob (7) of clam member (6), make rectangular glass (5) fixing and form a groove of opening above with central frame (1) top; Following bottom land seat (12) is a rectangle shallow slot, and the cell body opening size cooperates with last groove body, and groove body is inserted wherein just.
2. the novel electrophoresis tank that has cooling chamber according to claim 1 is characterized in that, U type breach glass plate (3) outer surface is handled through silanization and is hydrophobic state, and its U type breach matches with the U type mouth on central frame (1) top.
3. the electrophoresis tank that has cooling chamber according to claim 1, it is characterized in that, the groove that rectangular glass (5) and central frame (1) top form, groove depth is 10-40mm, bottom land axis distribution one row's platinum filament fixed pivot (10), last groove platinum filament (11) suspension is on it, and apart from bottom land 1-2mm, an end of last groove platinum filament (11) is connected in the last groove binding post (8) of central frame (1) end.
4. the electrophoresis tank that has cooling chamber according to claim 1, it is characterized in that, following bottom land seat (12) groove depth is 20-60mm, and axis, bottom land surface stationkeeping has following groove platinum filament (14), and an end of following groove platinum filament (14) is connected in down the following groove binding post (13) of bottom land seat (12) end.
5. the electrophoresis tank that has cooling chamber according to claim 1 is characterized in that, a side bonds rectangle organic glass blind plate (15) of described central frame (1) substitutes a series of superimposed layers of this side, forms single face glue cell structure.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009202580129U CN201522476U (en) | 2009-11-10 | 2009-11-10 | Electrophoresis tank with cooling chamber |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009202580129U CN201522476U (en) | 2009-11-10 | 2009-11-10 | Electrophoresis tank with cooling chamber |
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| Publication Number | Publication Date |
|---|---|
| CN201522476U true CN201522476U (en) | 2010-07-07 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN2009202580129U Expired - Fee Related CN201522476U (en) | 2009-11-10 | 2009-11-10 | Electrophoresis tank with cooling chamber |
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| CN (1) | CN201522476U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105259242A (en) * | 2015-05-18 | 2016-01-20 | 上海交通大学 | Online capillary electrophoresis box apparatus and method thereof |
-
2009
- 2009-11-10 CN CN2009202580129U patent/CN201522476U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105259242A (en) * | 2015-05-18 | 2016-01-20 | 上海交通大学 | Online capillary electrophoresis box apparatus and method thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Assignee: Henan Kerui Science and Technology Co., Ltd. Assignor: Zhang Liqun Contract record no.: 2011410000054 Denomination of utility model: Electrophoresis tank with cooling cavity Granted publication date: 20100707 License type: Exclusive License Record date: 20110422 |
|
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20100707 Termination date: 20131110 |