CN201465393U - Experimental apparatus for filter paper chromatography - Google Patents
Experimental apparatus for filter paper chromatography Download PDFInfo
- Publication number
- CN201465393U CN201465393U CN2009200598946U CN200920059894U CN201465393U CN 201465393 U CN201465393 U CN 201465393U CN 2009200598946 U CN2009200598946 U CN 2009200598946U CN 200920059894 U CN200920059894 U CN 200920059894U CN 201465393 U CN201465393 U CN 201465393U
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- CN
- China
- Prior art keywords
- double dish
- filter paper
- rubber plug
- developping agent
- cylinder
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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- Treatment Of Liquids With Adsorbents In General (AREA)
Abstract
The utility model relates to an experimental apparatus for filter paper chromatography. A hole is drilled by taking a middle point of a connection line between a center point of a glass tank cover plate of a round chromatographic tank and any point at the edge as a center of a circle, and a conical rubber plug is inserted in the hole; the center of the rubber plug is provided with a hole passing through the upper surface and the lower surface of the rubber plug, and a long-neck sphere separating funnel is inserted in the hole; during the experimental operation of paper chromatography, two culture plates are placed on the inner bottom surface of the chromatographic tank, wherein a developing solvent is injected in one of the culture plates. The utility model has the advantages of simple structure, convenient use, no solvent and steam escape phenomenon, linear solvent front of an atlas of experimental, computable Rf and extremely high experimental success rate.
Description
Technical field
The utility model belongs to a kind of ply of paper and analyses experimental provision.
Background technology
At present, the experimental provision of " ply of paper is analysed experiment " generally is made up of circle tube glass container (to call chromatography cylinder in the following text) and the three parts such as double dish of internal diameter 80～100mm, high a 15～20mm about the sheet glass chromatography cylinder cover plate about a diameter 330mm (to call the cylinder cap plate in the following text), internal diameter 300mm in the known biochemistry teaching.During use, earlier double dish is placed on the inner bottom surface of chromatography cylinder, inject thick " developping agent " of 8～10mm in the double dish, again will be point sample and be rolled into the next door that filter paper cylindraceous vertically is placed on double dish, at cylinder flap upper cylinder half cover plate that chromatography cylinder is airtight then, interior space of the saturated chromatography cylinder of solvent vapo(u)r that allows the developping agent evaporation form and filter paper carry out " balance ".After balance finished, the operator opened the cylinder cap plate and stretches out one's hand in the chromatography cylinder, filter paper is picked up vertically put into double dish then, allowed developping agent flow from bottom to top along filter paper, added a cylinder cap plate lid mouthful airtight chromatography cylinder then and carried out " expansion ".In a single day open the cylinder cap plate in aforesaid operations, the solvent vapo(u)r in the chromatography cylinder is almost all run away, its consequence is to cause the solvent front of experimental patterns to be irregular curve-like and can't to calculate R
f, usually cause the failure of an experiment.
Summary of the invention
The purpose of this utility model provides a kind of ply of paper and analyses experimental provision, utilize the long-neck bulb shape separatory funnel under the airtight situation of chromatography cylinder, to add developping agent, filter paper is immersed in the developping agent, overcome in operation in case open the cylinder cap plate, solvent vapo(u)r in the chromatography cylinder is almost all run away, and its consequence is to cause the solvent front of experimental patterns to be irregular curve-like and can't to calculate R
f, usually cause the shortcoming of the failure of an experiment.
The utility model is by chromatography cylinder, double dish, developping agent, filter paper, the plate lid, the truncated cone-shaped rubber plug, bulb shape separatory funnel is formed, it is characterized in that on the inner bottom surface of chromatography cylinder, putting double dish, double dish, during balance, only at double dish dress developping agent, in the double dish of another no developping agent, vertically place point sample, and be rolled into filter paper cylindraceous, the chromatography cylinder cover plate covers at chromatography cylinder cylinder mouth, the truncated cone-shaped rubber plug is inserted in the circular hole of chromatography cylinder cover plate, truncated cone-shaped rubber plug central authorities insert bulb shape separatory funnel, the filler neck of separating funnel stretches into from the central authorities of cylindric filter paper, the lower liquid outlet of filler neck is close to the inner bottom surface of double dish, during expansion, open the switch of bulb shape separatory funnel, allow developping agent in the lower liquid outlet inflow double dish of filler neck, launch.
The beneficial effects of the utility model are, and are simple in structure, easy to use, solvent-free steam escape phenomenon, and the solvent front of experimental patterns is linearly, can calculate R
f, experiment success rate is high.
Description of drawings
Fig. 1 is that the utility model balance is carried out the central fore-and-aft vertical plane structural map;
Fig. 2 is that the utility model launches to carry out the central fore-and-aft vertical plane structural map.
1 bulb shape separatory funnel among the figure, the 2nd, separating funnel switch, the 3rd, cylinder cap plate, the 4th, chromatography cylinder, the 5th, double dish, the 6th, round table-like rubber plug, the 7th, filler neck, the 8th, point sample filter paper, the 9th, double dish, the 10th, developping agent.
Embodiment
The technical scheme that its technical matters that solves the utility model adopts is: with the central point of above-mentioned glass jar cover plate and edge arbitrarily a bit between the mid point of line be the hole that a diameter 37mm is bored in the center of circle, diameter 40mm above in the hole, inserting one, following diameter 35mm, the truncated cone-shaped rubber plug of high 35mm.Bore one in these rubber plug central authorities and run through the above and below, the hole of diameter 10mm, the long-neck bulb shape separatory funnel of a 300ml of insertion in the hole.Carry out ply of paper when analysing experimental implementation, on the inner bottom surface of chromatography cylinder, putting into earlier the double dish of two internal diameter 80～100mm, high 15～20mm, injecting the thick developping agent of about 10mm in one of them double dish.Will be point sample and be rolled into filter paper cylindraceous, vertically be placed in the double dish of another no developping agent, the point sample end down.Then the cylinder cap plate is covered, make chromatography cylinder airtight, and the filler neck of separating funnel is positioned on the central authorities of tubular filter paper and the inner bottom surface that lower liquid outlet is close to double dish, close separating funnel, the solvent vapo(u)r that allows developping agent in the chromatography cylinder produce carries out " balance ".Balance is filled it up with developping agent after finishing in separating funnel, open switch, and developping agent is slowly flowed into downwards in the double dish, when the high 10mm of developping agent in the double dish left and right sides, closes separating funnel and carries out " expansion ", finishes up to experiment.
In examples of implementation shown in Figure 1 " balance carry out in " step, double dish 5, double dish 9 is placed in the chromatography cylinder 4, the thick developping agent of the about 10mm of dress in the double dish 9, cylinder cap plate 3 covers on the openning of chromatography cylinder 4, truncated cone-shaped rubber plug 6 is inserted in the circular hole of cylinder cap plate 3, bulb shape separatory funnel 1 is through the circular hole of truncated cone-shaped rubber plug 6 centres, point sample, and be rolled in the double dish 5 that filter paper 8 cylindraceous vertically is placed on no developping agent, the point sample end down, cylinder cap plate 3 covered make chromatography cylinder 4 airtight, and the filler neck 7 that makes bulb shape separatory funnel 1 just in time is positioned at the central authorities of cylindric filter paper 8, the lower liquid outlet of separating funnel 1 is close on the inner bottom surface of double dish 5, shuts separating funnel switch 2.
In examples of implementation shown in Figure 2 " expansion carry out in " step, double dish 5, double dish 9 is placed in the chromatography cylinder 4, the thick developping agent of the about 10mm of dress in the double dish 9, cylinder cap plate 3 covers on the openning of chromatography cylinder 4, truncated cone-shaped rubber plug 6 is inserted in the circular hole of cylinder cap plate 3, bulb shape separatory funnel 1 is through the circular hole of truncated cone-shaped rubber plug 6 centres, point sample, and be rolled in the double dish 5 that filter paper 8 cylindraceous vertically is placed on no developping agent, the point sample end down, cylinder cap plate 3 covered make chromatography cylinder 4 airtight, and the filler neck 7 that makes bulb shape separatory funnel 1 just in time is positioned at the central authorities of cylindric filter paper 8, the lower liquid outlet of separating funnel 1 is close on the inner bottom surface of double dish 5, shuts separating funnel switch 2." balance " step is filled developping agent after finishing in separating funnel 1, open separating funnel switch 2, allow developping agent slowly flow into double dish 5, when the about 10mm of developping agent liquid level is high, close separating funnel switch 2 along the lower liquid outlet of filler neck 7, carry out " expansion ", finish up to experiment.
Claims (1)
1. a ply of paper is analysed experimental provision, by chromatography cylinder, double dish, developping agent, filter paper, the plate lid, the truncated cone-shaped rubber plug, bulb shape separatory funnel is formed, it is characterized in that on the inner bottom surface of chromatography cylinder (4), putting double dish (5), double dish (9), during balance, only at double dish (9) dress developping agent (10), in the double dish (5) of another no developping agent (10), vertically place point sample, and be rolled into filter paper cylindraceous (8), chromatography cylinder (4) cover plate (3) covers at chromatography cylinder (4) cylinder mouth, truncated cone-shaped rubber plug (6) is inserted in the circular hole of chromatography cylinder (4) cover plate (3), truncated cone-shaped rubber plug (6) central authorities insert bulb shape separatory funnel (1), the filler neck (7) of separating funnel (1) stretches into from the central authorities of cylindric filter paper (8), the lower liquid outlet of filler neck (7) is close to the inner bottom surface of double dish (5), during expansion, open the switch (2) of bulb shape separatory funnel (1), allow developping agent (10) in the lower liquid outlet inflow double dish (5) of filler neck (7), launch.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009200598946U CN201465393U (en) | 2009-07-01 | 2009-07-01 | Experimental apparatus for filter paper chromatography |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009200598946U CN201465393U (en) | 2009-07-01 | 2009-07-01 | Experimental apparatus for filter paper chromatography |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201465393U true CN201465393U (en) | 2010-05-12 |
Family
ID=42392741
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2009200598946U Expired - Fee Related CN201465393U (en) | 2009-07-01 | 2009-07-01 | Experimental apparatus for filter paper chromatography |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201465393U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102288731A (en) * | 2011-07-26 | 2011-12-21 | 江苏金辰针纺织有限公司 | Method for testing rate of flow value of reactive dye |
| CN108470497A (en) * | 2018-04-27 | 2018-08-31 | 长江大学 | A kind of ultrasonic image logging experimental provision containing two-dimentional slip scan platform |
-
2009
- 2009-07-01 CN CN2009200598946U patent/CN201465393U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102288731A (en) * | 2011-07-26 | 2011-12-21 | 江苏金辰针纺织有限公司 | Method for testing rate of flow value of reactive dye |
| CN108470497A (en) * | 2018-04-27 | 2018-08-31 | 长江大学 | A kind of ultrasonic image logging experimental provision containing two-dimentional slip scan platform |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20100512 Termination date: 20110701 |