CN201394004Y - Up-conversion luminescence in-vivo rat imaging system of steady-state laser pumping - Google Patents
Up-conversion luminescence in-vivo rat imaging system of steady-state laser pumping Download PDFInfo
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- CN201394004Y CN201394004Y CN2009200717362U CN200920071736U CN201394004Y CN 201394004 Y CN201394004 Y CN 201394004Y CN 2009200717362 U CN2009200717362 U CN 2009200717362U CN 200920071736 U CN200920071736 U CN 200920071736U CN 201394004 Y CN201394004 Y CN 201394004Y
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Abstract
The utility model relates to an up-conversion luminescence in-vivo rat imaging system of steady-state laser pumping. The imaging system comprises a semiconductor laser, an expanded beam lens, a beam shaping lens, a sample holder, a cut off filter, a camera lens, an emission filter, and a CCD detector, and is characterized in that the emergent lights of the semiconductor laser aim at the light inlet port of the expanded beam lens; the light outlet port of the expanded beam lens aims at the light inlet port of the beam shaping lens; the light outlet port of the beam shaping lens aims at the sample holder; the cut off filter, of which the light outlet port aims at the light inlet port of the camera lens, is arranged right above the sample holder; the light outlet port of the camera lens aimsat the light inlet port of the emission filter; and the light outlet port of the emission filter aims at the CCD detector. The utility model can directly conduct safe in-vivo imaging in a real-time manner on the material with up-conversion luminescence characteristic of steady-state laser pumping, thereby revealing the motion at the molecular level.
Description
Technical field
This utility model relates to a kind of molecular imaging optical imaging apparatus, particularly a kind of mesomeric state laser pump up-conversion luminescence toy living imaging system.Can carry out real-time, safe imaging to the live body (mice) of the material of labelling up-conversion luminescence character, can directly monitor intravital cellular activity of living body biological and gene behavior.By this system, can observe the biological processes such as expression of the growth of living animal in-vivo tumour and transfer, infectious disease evolution, specific gene.
Background technology
Living animal fluorescence imaging technology allows research worker can observe intravital gene expression of living animal and cellular activity, be that molecule and cytobiology technology are developed into the intravital strong means of living animal from vitro study, be applied to medical science and biological study field more and more widely.With respect to other Noninvasive Observations Means, as CT, MRI, and PET etc., the sensitivity of fluorescence imaging technology for detection is high, and simple to operate, and expense is cheap relatively, for research worker provides wide application space.But traditional fluorescent probe all has its inherent defective, as fluorescence organic dyestuff and fluorescin molecule photobleaching takes place easily, therefore can't realize preferably the long time fluorescent labeling of research molecule is observed.And the variable characteristic on the toxicity of quantum dot, surface and associated " flicker " (Blinking) phenomenon to make that quantum dot is difficult to use in clinical.In addition, these materials all belong to the conjugated system pi-electron and excite and launch, with the autofluorescence similar process of biological sample endogenous fluorescent material, when therefore using these luminescent materials to carry out the living imaging detection, often exist the interference of the autofluorescence of biological sample as biomarker.Therefore seeking advanced luminescent material, to avoid background fluorescence as biomarker be crucial difficult point in the living body fluorescent imaging technique.
Recently, the application of the up-conversion luminescent material of mesomeric state laser pump in bio-imaging caused the attention of more and more researcheres.Compare with traditional fluorescent material, this class material has special up-conversion luminescence character, can absorb two or more lower energy photons and high-energy photon of radiation normally converts near infrared light to visible light.Adopt near infrared light as exciting light, can reduce the damage of exciting light, improve imaging depth, and use cheap steady laser (～$2,000) to greatly reduce the instrument cost biological sample.In addition, because biological sample endogenous fluorescent material can not be excited by the stable state near-infrared laser, use this class material can eliminate the interference of biological sample autofluorescence fully.Therefore, if can be with the up-conversion luminescent material of mesomeric state laser pump with since the fluorescence imaging technology combines, develop a kind of novel living imaging system, then be expected to solve some problems that exist in the existing fluorescence imaging, for life sciences, medical science and material science research provide a kind of new method.
Summary of the invention
The purpose of this utility model is to provide a kind of mesomeric state laser pump up-conversion luminescence toy living imaging system, this system can be directly carries out real-time, safe imaging to the live body (mice) of the material of labelling up-conversion luminescence character, can directly monitor intravital cellular activity of living body biological and gene behavior.By this system, can also observe the biological processes such as expression of the growth of living animal in-vivo tumour and transfer, infectious disease evolution, specific gene.
The mesomeric state laser pump up-conversion luminescence toy living imaging system that the utility model proposes, by semiconductor laser 1, extender lens 2, beam shaping mirror 3, sample platform 4, edge filter 5, camera lens 6, emission optical filter 7 and CCD detector 8 are formed, wherein, the emergent light of semiconductor laser 1 is aimed at the light inlet of extender lens 2, the light inlet of the light-emitting window alignment shaping mirror 3 of extender lens 2, the light-emitting window of beam shaping mirror 3 is aimed at sample platform 4, be placed with edge filter 5 directly over the sample platform 4, the light-emitting window of edge filter 5 is aimed at the light inlet of camera lens 6, the light-emitting window of camera lens 6 is aimed at the light inlet of emission optical filter 7, and the light-emitting window of emission optical filter 7 is aimed at CCD detector 8.
In this utility model, it is the semiconductor laser of 980nm that semiconductor laser 1 adopts centre wavelength.
In this utility model, edge filter 5 adopts the 980nm edge filter.
In this utility model, the quantity of semiconductor laser 1 is 1-4, is determined on a case-by-case basis, and is corresponding, and extender lens 2 is 1-4 with the quantity of beam shaping mirror 3.
In this utility model, extender lens 2, beam shaping mirror 3, sample platform 4, edge filter 5, camera lens 6, emission optical filter 7 and CCD detector 8 are positioned at imaging camera bellows 10.
In this utility model, toy 11 is positioned on the sample platform 4.
In this utility model, sample platform 4 connects motor.
In this utility model, CCD detector 8 connects computer 9.
This utility model can directly carry out real-time, safe living imaging to the material with mesomeric state laser pump up-conversion luminescence character, thereby discloses the activity of molecular level.
Work process of the present utility model is: the steady laser bundle that semiconductor laser 1 produces is through extender lens 2 and beam shaping mirror 3, become a branch of bigger collimated light beam, be radiated on the toy 11, the material that has mesomeric state laser pump up-conversion luminescence character in toy 11 bodies is launched the up-conversion luminescence along all directions under the exciting of laser, part up-conversion luminescence signal is collected by camera lens 6 by edge filter 5 and is converted collimated light beam to, again by emission optical filter 7, the signal of certain wave-length coverage is intercepted, and is received by CCD detector 8 then.
This utility model is compared with existing living body fluorescent imaging technique, have the following advantages: 1. owing to biological sample endogenous fluorescent material and organic fluorescent dye commonly used can not be excited by the stable state near infrared laser, therefore this utility model has been eliminated the interference of background fluorescences such as biological sample autofluorescence and organic fluorescent dye be luminous, is a kind of highly sensitive imaging technique.2. the up-conversion luminescent material owing to mesomeric state laser pump excites down hardly by photobleaching at the stable state near-infrared laser, and the stable state near-infrared laser is very weak to the photobleaching of organic fluorescent dye, damage to biological sample is little, so this utility model is a kind of imaging technique that can carry out continuous observation of long period to biological sample.3. this utility model adopts semiconductor laser as excitation source, compares with the femto-second laser that the two-photon fluorescence probe adopts, and semiconductor laser domesticizes, and is cheap, so this utility model is easy to popularize.
Description of drawings
Fig. 1 is a basic structure sketch map of the present utility model.
Fig. 2 is the structural representation of an example of the present utility model.
Fig. 3 uses the mesomeric state laser pump up-conversion luminescent material tail vein injection of this utility model embodiment 1 acquisition to the intravital imaging picture of kunming mice.(a) mice White-light image (b) is up-conversion luminescence and mice white light superimposed image.
Fig. 4 is the intravital imaging picture of nude mice that arrives the band tumor with the mesomeric state laser pump up-conversion luminescent material tail vein injection that this utility model embodiment 1 obtains.(a) mice White-light image (b) is up-conversion luminescence and mice white light superimposed image.
Number in the figure: 1 is semiconductor laser, and 2 is extender lens, and 3 is the beam shaping mirror, and 4 is the sample platform, and 5 is edge filter, and 6 is camera lens, and 7 are the emission optical filter, and 8 is the CCD detector, and 9 is computer, and 10 are the imaging camera bellows, and 11 is toy.
The specific embodiment
The utility model is described in further detail below in conjunction with drawings and Examples, rather than be used for limiting scope of the present utility model.
As shown in Figure 1, the steady laser bundle that semiconductor laser (centre wavelength is 980nm) 1 produces imports by optical fiber, along placing extender lens 2, beam shaping mirror 3 on this laser beam direction of advance successively, placing sample platform 4 along this laser beam side-lower, above this sample platform, be placed with 980nm edge filter 5 successively coaxially, camera lens 6, emission optical filter 7 and CCD detector 8.
As shown in Figure 2, the steady laser Shu Jun that two semiconductor lasers (centre wavelength is 980nm) 1 produce imports by optical fiber, along placing extender lens 2, beam shaping mirror 3 on the laser beam direction of advance successively, wherein extender lens 2 is connected with a joint arm, angle that can free adjustment fiber port light beam, place sample platform 4 at two bundle laser beam side-lowers, toy 11 is positioned on the sample platform.Above this sample platform, be placed with 980nm edge filter 5 successively coaxially, camera lens 6, emission optical filter 7 and CCD8.Wherein CCD8 is connected with computer 9.Semiconductor laser (centre wavelength is 980nm) 1, extender lens 2, beam shaping mirror 3, sample platform 4,980nm edge filter 5, camera lens 6, emission optical filter 7 and CCD8 etc. become light path to connect successively.Can the comprehensive irradiation living small animal of 360 degree by regulating joint arm, select the signal of certain wave-length coverage to receive by emission optical filter 7 by CCD8, computer 9 is connected with CCD8, and the realization image is stored and scan control.In addition, driving sample platform 4 by stepper motor moves along the Z axle.In the present embodiment, it is the adjustable homemade laser instrument of 0-6.5W (Shanghai vast space Fibre Optical Communication Technology company limited) that semiconductor laser (centre wavelength is 980nm) adopts output, 660nm+/-11 bandpass filters and 800nm+/-12 bandpass filters (cold spring scientific ﹠ technical corporation), detector is Andor DU897 EMCCD, and image storage, analysis software are Kodak Molecular Imaging Software.
The work process of present embodiment is:
The laser beam that semiconductor laser (centre wavelength is 980nm) 1 sends is through extender lens 2 and beam shaping mirror 3, become the bigger collimated light beam of a beam diameter, be radiated on the living small animal 11 from the side top, the material that has mesomeric state laser pump up-conversion luminescence character in toy 11 bodies is launched the up-conversion luminescence along all directions under the exciting of laser, part up-conversion luminescence signal is collected by camera lens 6 by 980nm edge filter 5, is received by CCD8 by emission optical filter 7 again.Carry out Flame Image Process by computer 9 then.
Fig. 3 is the mesomeric state laser pump up-conversion luminescent material that obtains with embodiment of the present utility model by tail vein injection imaging picture of mice after 24 hours in the kunming mice body.(a) mice White-light image is to excite the up-conversion luminescence image of acquisition down and the stack of mice White-light image in steady laser (b).
Fig. 4 is the mesomeric state laser pump up-conversion luminescent material that obtains with embodiment of the present utility model by tail vein injection imaging picture of mice after 24 hours in the nude mouse of band tumor.(a) mice White-light image is to excite the up-conversion luminescence image of acquisition down and the stack of mice White-light image in steady laser (b).
Claims (8)
1, a kind of mesomeric state laser pump up-conversion luminescence toy living imaging system, by semiconductor laser (1), extender lens (2), beam shaping mirror (3), sample platform (4), edge filter (5), camera lens (6), emission optical filter (7) and CCD detector (8) are formed, the light inlet that it is characterized in that the emergent light aligning extender lens (2) of semiconductor laser (1), the light inlet of the light-emitting window alignment shaping mirror (3) of extender lens (2), the light-emitting window of beam shaping mirror (3) is aimed at sample platform (4), be placed with edge filter (5) directly over the sample platform (4), the light-emitting window of edge filter (5) is aimed at the light inlet of camera lens (6), the light-emitting window of camera lens (6) is aimed at the light inlet of emission optical filter (7), and the light-emitting window of emission optical filter (7) is aimed at CCD detector (8).
2, mesomeric state laser pump up-conversion luminescence toy living imaging according to claim 1 system is characterized in that it is the semiconductor laser of 980nm that semiconductor laser (1) adopts centre wavelength.
3, mesomeric state laser pump up-conversion luminescence toy living imaging according to claim 1 system is characterized in that edge filter (5) adopts the 980nm edge filter.
4, mesomeric state laser pump up-conversion luminescence toy living imaging according to claim 1 system, the quantity that it is characterized in that semiconductor laser (1) is 1-4, and is corresponding, extender lens (2) is 1-4 with the quantity of beam shaping mirror (3).
5, mesomeric state laser pump up-conversion luminescence toy living imaging according to claim 1 system is characterized in that extender lens (2), beam shaping mirror (3), sample platform (4), edge filter (5), camera lens (6), emission optical filter (7) and CCD detector (8) are positioned at imaging camera bellows (10).
6, mesomeric state laser pump up-conversion luminescence toy living imaging according to claim 1 system is characterized in that toy (11) is positioned on the sample platform (4).
7, mesomeric state laser pump up-conversion luminescence toy living imaging according to claim 1 system is characterized in that sample platform (4) connects motor.
8, mesomeric state laser pump up-conversion luminescence toy living imaging according to claim 1 system is characterized in that CCD detector (8) connects computer (9).
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| Application Number | Priority Date | Filing Date | Title |
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| CN2009200717362U CN201394004Y (en) | 2009-05-07 | 2009-05-07 | Up-conversion luminescence in-vivo rat imaging system of steady-state laser pumping |
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| CN2009200717362U CN201394004Y (en) | 2009-05-07 | 2009-05-07 | Up-conversion luminescence in-vivo rat imaging system of steady-state laser pumping |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105527265A (en) * | 2016-01-22 | 2016-04-27 | 复旦大学 | Laser pump time resolution up-conversion luminescence living imaging system |
| CN109813687A (en) * | 2017-11-22 | 2019-05-28 | 中国科学院宁波材料技术与工程研究所 | A kind of imaging method of up-conversion fluorescence imaging system and up-conversion fluorescence |
| CN112998656A (en) * | 2021-02-24 | 2021-06-22 | 广东工业大学 | Living body fluorescence imaging system capable of intelligently tracking target and control method thereof |
-
2009
- 2009-05-07 CN CN2009200717362U patent/CN201394004Y/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105527265A (en) * | 2016-01-22 | 2016-04-27 | 复旦大学 | Laser pump time resolution up-conversion luminescence living imaging system |
| CN105527265B (en) * | 2016-01-22 | 2023-07-04 | 复旦大学 | Laser pumping time resolution up-conversion luminescence living body imaging system |
| CN109813687A (en) * | 2017-11-22 | 2019-05-28 | 中国科学院宁波材料技术与工程研究所 | A kind of imaging method of up-conversion fluorescence imaging system and up-conversion fluorescence |
| CN112998656A (en) * | 2021-02-24 | 2021-06-22 | 广东工业大学 | Living body fluorescence imaging system capable of intelligently tracking target and control method thereof |
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