CN201311376Y

CN201311376Y - Freezing microtome

Info

Publication number: CN201311376Y
Application number: CNU2008201923606U
Authority: CN
Inventors: 朱以印
Original assignee: Individual
Current assignee: HUBEI LEIKE MEDICAL INSTRUMENT Co Ltd
Priority date: 2008-11-09
Filing date: 2008-11-09
Publication date: 2009-09-16
Anticipated expiration: 2018-11-09

Abstract

The utility model discloses a freezing microtome which belongs to the technical field of an auxiliary device for detecting medical biological tissue. The freezing microtome mainly solves the problem that 2 to 3 days are needed for pathology examination of corpse sample, which can not meet the requirements of the clinical diagnosis during operation. The freezing microtome is characterized in that the freezing microtome comprises a manual microtome section device and further comprises a cabinet provided with a refrigerating chamber, a refrigeration system is installed in the cabinet, and a viewing glass double acting door is adopted; the manual microtome section device is installed in the refrigerating chamber, wherein, a big hand wheel and a connecting shaft are combined and mounted on the outer side of the cabinet through a transmission shaft connected with the main shaft; the manual microtome section device adopts an automatic cutter advancement device consisting of a micro motor, a micro switch, a long gear, a shift lever and a shifting gear, and wherein, the shift lever and the shifting gear are connected with a cassette mechanism; and a sample refrigeration rack is installed inside the refrigerating chamber. The utility model has the characteristic of being capable of quickly performing quick freezing and microtome section on fresh and alive biological tissue, and is mainly used for frozen section on the fresh and alive biological tissue collected during an operation.

Description

Freezing-microtome

Technical field

The utility model belongs to medical bio tissue detection servicing unit technical field.Be specifically related to a kind of tissue samples snap frozen section in the hospital surgical that is mainly used in, in 30 minutes, diagnose the freezing-microtome that reliable pathological diagnosis foundation is provided to the state of an illness.

Background technology

In the pathological diagnosis process, generally all need diseased region is taken a sample, with sample by processing procedures such as dehydration, dyeing, embedding, section, baking sheets, then histotomy is placed under the high-power microscope and observes, thereby pathology is made diagnosis, generally this process all needs 2-3 days time, this can meet the demands for the diagnosis pathology, and, then requiring to judge fast the pathology type for the diagnosis in the surgical procedure, this can not meet the needs of far away.

Summary of the invention

The purpose of this utility model is exactly to provide a kind of can carry out the snap frozen section to tissue samples in 30 minutes at above-mentioned weak point, thereby the freezing-microtome of state of an illness diagnosis basis is provided to operation.

Technical solution of the present utility model is: a kind of freezing-microtome, comprise the hand section device that contains base plate, casing, movement, gear set, big handwheel, sample folder, blade holder, it is characterized in that: also comprise a rack that contains refrigerating chamber, refrigeration system is housed in the rack, and adopts observable glass sliding door; The hand section device is loaded in the refrigerating chamber, and wherein, big handwheel is loaded on the rack outside by transmission shaft and the connecting shaft knot that is connected with main shaft; The hand section device adopts the automatic feeder that is made of micromotor, microswitch, spool gear, driving lever and conversion gear, and wherein, driving lever is connected with movement with conversion gear; Freezing of sample is housed in the refrigerating chamber.

Freezing of sample described in the utility model technical solution comprises base, column, connection strap, handgrip, vertical rod, briquetting and sample disk; Base is provided with sample disk seat hole; Column is vertically fixed on the base; Connection strap is horizontally fixed on the column upper end; Handgrip, vertical rod and briquetting are connected successively, and are fixed on the connection strap.

Heating film is installed around the refrigerating chamber top described in the utility model technical solution.

The utility model is owing to adopt the rack that contains refrigerating chamber, and freezing of hand section device and sample be housed in the rack refrigerating chamber, wherein, big handwheel is loaded on the rack outside by transmission shaft and the connecting shaft knot that is connected with main shaft, thereby when using, after freezer temperature reaches about-35 ℃, to gather the fresh and alive biological tissue that comes earlier is bonded on the sample disk with bonding agent, this sample disk is put on the sample disk seat hole again, and compress with briquetting, sample tissue is promptly pressed the lump that is frozen into for section usefulness after about 2-3 minute, at last sample disk is taken out the sample folder of putting into the hand section device and finishes section, can carry out pathological examination afterwards.The utility model has overcome the deficiencies in the prior art part, has the advantages that fresh and alive biological tissue to be carried out snap frozen and section, the pathological examination of the fresh and alive tissue samples that is specially adapted to gather in operation.The utility model is mainly used in the freezing microtome section of the fresh and alive tissue samples that collects in the operation.

Description of drawings

Fig. 1 is a main TV structure synoptic diagram of the present utility model;

Fig. 2 is the left TV structure synoptic diagram of Fig. 1;

Fig. 3 is the right TV structure synoptic diagram of Fig. 1;

Fig. 4 is the structural representation of freezing of sample;

Fig. 5 is the plan structure synoptic diagram of Fig. 4;

Fig. 6 is the structural representation of sample disk;

Fig. 7 is the plan structure synoptic diagram of Fig. 6;

Fig. 8 is the structural representation of automatic feeder;

Fig. 9 is the left TV structure synoptic diagram of Fig. 8;

Figure 10 is the control circuit figure of automatic feeder;

Figure 11 is the circuit diagram of temperature control system;

Figure 12 is the structural representation of heating film.

Embodiment

As shown in Figure 1 to Figure 3.Cabinet 3 tops are provided with refrigerating chamber, and refrigerating chamber is provided with thermofin 10 and glass sliding door 4 outward, and the glass sliding door is convenient to observe indoor conditions.Refrigeration system adopts conventional system.Compressor 12 in the refrigeration system, fan 13, condenser 14 are loaded on cabinet 3 bottoms, and evaporator 7 and heating film 9 are loaded in the refrigerating chamber.Freezing 11 in hand section device 5 and sample are loaded in the refrigerating chamber.Hand section device 5 is made of base plate, casing, movement, gear set, big handwheel 6, sample folder, blade holder, automatic feeder 15, wherein, corresponding identical in base plate, casing, movement, gear set, big handwheel 6, sample folder, blade holder and the existing normal temperature hand section device, different is adopts the automatic feeder 15 and the installation site of handwheel 6 greatly.Big handwheel 6 is loaded on rack 3 outsides by transmission shaft and the connecting shaft knot that is connected with main shaft, is convenient to operation, meets the bodily fuctions and learns principle.

Extremely shown in Figure 7 as Fig. 4.Freezing 11 in sample comprises base 21, column 18, connection strap 17, handgrip 16, vertical rod 19, briquetting 20 and sample disk 23; Base 21 is provided with sample disk seat hole 22; Column is vertically fixed on the base; Connection strap is horizontally fixed on the column upper end; Handgrip, vertical rod and briquetting are connected successively, and are fixed on the connection strap.Base is provided with four sample disk seat holes 22 ((1), (2), (3), (4)).Sample disk top is disk, and the bottom is a support column, and support column matches with sample disk seat hole.

As Fig. 8 and shown in Figure 9.Autofeed (withdrawing) device is made of micromotor 25, microswitch 26, spool gear 27, driving lever 28 and conversion gear 29.Wherein driving lever 28 is connected with movement with conversion gear 29.Micromotor 25 directly is connected on the spool gear 27 of movement 24 bottoms, after the energising, micromotor 25 drives spool gear 27 and rotates realization advance and retreat cutter.Two microswitches 26 are housed on movement 24 simultaneously, plectrum 30 and contact 31 are arranged on the microswitch 26, the plectrum direction as shown in the figure.When pressing the feed key, motor just changes, and realizes feed, and when feed put in place, the driving lever on the movement 24 was depressed plectrum, and plectrum is pressed upper contact, and microswitch 26 is disconnected, and micromotor 25 stalls stop feed.When pressing the withdrawing button, the motor counter-rotating realizes withdrawing, and when withdrawing put in place, the driving lever on the movement 24 was depressed the plectrum on another microswitch 26, and plectrum is pressed upper contact, and microswitch 26 is disconnected, and micromotor 25 stalls stop withdrawing.

Automatically advance (moving back) cutter control circuit, as shown in figure 10, this circuit is made up of power supply 32, central die 33 and control circuit 34.Power supply 32 adopts full-wave rectification and 7805 voltage-stabilizing devices, alternating current is become direct current 24V and 5V voltage, for micromotor and chip, central die adopts 18F508, wherein 1 pin and 8 pin are 5V power input mouth, 4,5,6,7 pin are the advance and retreat cutter signal input port that button is come, and 2,3 pin are output port, send signal to control circuit 34.Control circuit has into (moving back) cutter two parts symmetric circuit, is made up of photoelectric coupled device 521 and triode 8050,8550.Enter this circuit

input end mouth

2 and 3 from the signal of

central die

2,3 pin output, just (bearing) 1,2 ends of voltage to J4 through output port output, the rotating of control micromotor realizes advancing automatically (moving back) cutter function.Whole process is by software control.

The temperature control system circuit as shown in figure 11.This circuit is made up of power supply 35, sensor input circuit 36, control circuit 37, output control circuit 38 4 parts.

Power supply adopts voltage-stabilizing device 7805, output dc voltage 5V.

Sensor adopts integrated biography temperature probe 18B20, and chip 4051 is adopted in input control; Governor circuit adopts central die 18F4520.

Output control circuit adopts 2 74HC595 chips, wherein makes the expansion mouth for one.

The signal of sensor 18B20 enters 1 pin of 4051 chips through the control utmost point 2 pin, export 33 pin of a signal by 4051 3 pin to central die 18F4520,7 human hair combing wastes of central die go out signal respectively to 13 pin of two 74HC595, wherein 15 pin of a 74HC595 expansion mouth promptly send the 1 port controlling relay of voltage signal to J2, control power of compressor then.

When temperature was higher than (being lower than) setting value, 18B20 output one was higher than the voltage signal of (being lower than) setting value, realized said process control by software, made compressor power disconnect (connection), and temperature is descended (rising), thereby reached the purpose of equilibrium temperature.

Temperature-control circuit gives and leaves 7 expansion interfaces (S2-S8), so that increase the sensing point for measuring temperature.Clock circuit 39 independently is used for time showing among Figure 11, does not do temperature control and uses.

As shown in figure 12.Owing to observe with the glass surfaces externally and internally temperature difference of sliding door 4 very big, its inside surface easily produces the mist pearl of condensation, for addressing this problem, be stained with four heating films 9 around above refrigerating chamber, heating film 9 is by film 40, heater strip 41 and go between and 42 form, 18 volts of heating voltages, power is respectively 5W (3) and 15W (1), when start, heating film 9 begins heating, when reaching equilibrium state, the heat that heating film 9 discharges makes the interior surfaces of glass temperature raise again, thereby eliminate the superincumbent mist pearl of condensation, make glass Clear ﹠ Transparent, the convenient observation and operation.

Claims

1, a kind of freezing-microtome, comprise the hand section device (5) that contains base plate, casing, movement, gear set, big handwheel (6), sample folder, blade holder, it is characterized in that: also comprise a rack (3) that contains refrigerating chamber, rack is equipped with refrigeration system in (3), and adopts observable glass sliding door (4); Hand section device (5) is loaded in the refrigerating chamber, and wherein, big handwheel (6) is loaded on rack (3) outside by transmission shaft and the connecting shaft knot that is connected with main shaft; Hand section device (5) adopts the automatic feeder (15) that is made of micromotor (25), microswitch (26), spool gear (27), driving lever (28) and conversion gear (29), and wherein, driving lever (28) is connected with movement with conversion gear (29); Freezing of sample (11) is housed in the refrigerating chamber.

2, a kind of freezing-microtome according to claim 1 is characterized in that: described sample freezing (11) comprises base (21), column (18), connection strap (17), handgrip (16), vertical rod (19), briquetting (20) and sample disk (23); Base (21) is provided with sample disk seat hole (22); Column (18) is vertically fixed on the base (21); Connection strap (17) is horizontally fixed on column (18) upper end; Handgrip (16), vertical rod (19) and briquetting (20) are connected successively, and are fixed on the connection strap (17).

3, a kind of freezing-microtome according to claim 1 and 2 is characterized in that: heating film (9) is installed around the described refrigerating chamber top.