CN201241157Y - Hap-dna纯化柱 - Google Patents

Hap-dna纯化柱 Download PDF

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Publication number
CN201241157Y
CN201241157Y CNU200820150699XU CN200820150699U CN201241157Y CN 201241157 Y CN201241157 Y CN 201241157Y CN U200820150699X U CNU200820150699X U CN U200820150699XU CN 200820150699 U CN200820150699 U CN 200820150699U CN 201241157 Y CN201241157 Y CN 201241157Y
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China
Prior art keywords
hap
inner prop
filter membrane
column
outer tube
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Expired - Lifetime
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CNU200820150699XU
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English (en)
Inventor
王启松
王志
李瑞峰
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Biological Engineering (Shanghai) Limited by Share Ltd
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SHANGHAI SHENGGONG BIOENGINEERING TECHNOLOGY SERVICE Co Ltd
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Abstract

本实用新型HAP－DNA纯化柱涉及一种生物工程用的纯化装置，尤其是指一种用于寡核苷酸引物纯化的HAP－DNA纯化柱，由外套管，内柱，滤膜和装在内柱里的HAP树脂填料层组成，外套管内部装有滤膜且端部有外接口，内柱两端分别与外套管连接。本实用新型HAP－DNA纯化柱具有结构简单、体积小、重量轻、高负载力、安装简单、正反方向均可使用的特点。

Description

HAP-DNA纯化柱
技术领域
本实用新型HAP-DNA(脱氧核酸高亲合纯化)纯化柱涉及一种生物工程用的纯化装置，尤其是指一种用于寡核苷酸引物纯化的柱体结构。
背景技术
目前国内绝大多数引物合成主要运用OPC柱纯化、HPLC纯化以及PAGE纯化这三种纯化方法，但是这三种纯化综合费用都较高。
发明内容
为了克服现有的纯化方法的不足，本实用新型的目的在于提供一种纯化寡核苷酸引物的HAP-DNA(脱氧核酸高亲合纯化)纯化柱。
本实用新型解决其技术问题所采用的技术方案是：一种HAP-DNA纯化柱，由外套管、内柱、滤膜和装在内柱里填料层组成，外套管内部装有滤膜且端部有外接口，内柱内为HAP(高亲合纯化)树脂填料层，内柱上、下两端分别与外套管连接。
其中滤膜可以是微孔滤膜，内柱和外套管的材料均为聚丙烯材料；外套管具有保护内柱并有效的与外界连接的作用；内柱起到衔接上下两个外套管及存放HAP树脂的作用；过滤膜能有效的过滤一些杂质，以防流入内柱，并且防止HAP树脂外流现象发生。
本实用新型的优点在于：结构简单、体积小、重量轻、具有较高负载能力、安装简单，使用时正反方向均可运用。
附图说明
图1为本实用新型的结构剖面图。
图2为本实用新型的分解结构剖面图。
附图中标号说明
1-外套管        11-外接口
12-滤膜         2-内柱
21-HAP树脂填料层
具体实施方式
下面结合附图进一步说明是如何实现本实用新型的，如图1、图2和图3所示：一种用于纯化合成引物的HAP-DNA纯化柱，由上、下外套管1，内柱2，滤膜12和装在内柱里的HAP树脂填料层21组成，外套管1上下两端各有一层滤膜12，内柱上、下两端分别与外套管连接。
其中内柱2和外套管1由聚丙烯材料制成，滤膜12可以是微孔滤膜。

Claims (3)

1、一种HAP-DNA纯化柱，由外套管、内柱、滤膜和装在内柱里的填料层组成，其特征在于：外套管内部装有滤膜且端部有外接口，内柱内为HAP树脂填料层，内柱上、下两端分别与外套管连接。
2、根据权利要求1所述的一种HAP-DNA纯化柱，其特征在于：所述内柱和外套管的材料为聚丙烯材料。
3、根据权利要求1或2所述的一种HAP-DNA纯化柱，其特征在于：所述滤膜为微孔滤膜。
CNU200820150699XU 2008-07-10 2008-07-10 Hap-dna纯化柱 Expired - Lifetime CN201241157Y (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CNU200820150699XU CN201241157Y (zh) 2008-07-10 2008-07-10 Hap-dna纯化柱

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CNU200820150699XU CN201241157Y (zh) 2008-07-10 2008-07-10 Hap-dna纯化柱

Publications (1)

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CN201241157Y true CN201241157Y (zh) 2009-05-20

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020104390A3 (en) * 2018-11-19 2020-07-23 Biocartis Nv Enhanced detection of low-copy-number nucleic acids in an integrated workflow

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020104390A3 (en) * 2018-11-19 2020-07-23 Biocartis Nv Enhanced detection of low-copy-number nucleic acids in an integrated workflow

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Owner name: SANGON BIOLOGICAL ENGINEERING (SHANGHAI) CO., LTD.

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Address after: 201611 Shanghai Songjiang District Chedun Industrial Zone, Hong Min Road No. 698

Patentee after: Sangon Biotech (Shanghai) Co., Ltd.

Address before: 201611 No. 698 Xiang min Road, Songjiang industrial district, Shanghai, China

Patentee before: shanghai Shenggong Bioengineering Technology Service Co., Ltd.

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Address after: 201611 Shanghai Songjiang District Chedun Industrial Zone, Hong Min Road No. 698

Patentee after: Biological Engineering (Shanghai) Limited by Share Ltd

Address before: 201611 Shanghai Songjiang District Chedun Industrial Zone, Hong Min Road No. 698

Patentee before: Sangon Biotech (Shanghai) Co., Ltd.

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Granted publication date: 20090520

CX01 Expiry of patent term