CN201184874Y - Molecular engram membrane electrode for detecting mycotoxins - Google Patents
Molecular engram membrane electrode for detecting mycotoxins Download PDFInfo
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- CN201184874Y CN201184874Y CNU2008200217460U CN200820021746U CN201184874Y CN 201184874 Y CN201184874 Y CN 201184874Y CN U2008200217460 U CNU2008200217460 U CN U2008200217460U CN 200820021746 U CN200820021746 U CN 200820021746U CN 201184874 Y CN201184874 Y CN 201184874Y
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Abstract
The utility model discloses a molecularly imprinted membrane electrode for fungal toxin detection. The molecularly imprinted membrane electrode comprises an electrode substrate and electrode matrixes, one of the electrode matrixes is connected with a working electrode as a whole through an electrode connecting wire by a terminal block, the other electrode matrix is connected with a counter electrode as a whole through the electrode connecting wire by the terminal block; a layer of polycarbonate is coated on the surface of each electrode connecting wire, and a reaction layer is coated on the working electrode. The molecularly imprinted membrane electrode further comprises a reference electrode which is fixed on the electrode substrate. A layer of carbon nanotubes for nano-synergy is further coated on the working electrode. The molecularly imprinted membrane electrode can realize the fast on-line detection of fungal toxin, the detection limit can be controlled at about 10min, the detection limit is 0.1ng/g; the carbon nanotubes are added in the reaction layer, thereby significantly improving the reaction sensitivity and the detection precision of the prepared electrode; the reference electrode is fixedly arranged on the electrode substrate, thereby allowing the detection to be more accurate.
Description
Technical field
The utility model relates to the biochemical electrode technical field by test electrochemical variable analysis of material, particularly a kind of molecular engram film electrode that is used for the mycotoxin detection.
Background technology
Mycotoxin (Mycotoxin) is the secondary metabolite that easily causes humans and animals pathological change and physiology metamorphosis that some fungies (being mainly aspergillus, Penicillium and Fusarium) produce in growth course, and toxicity is very high.Calculate by chemical constitution, estimating at 400 kinds of fungus metabolites is poisonous to the human and animal.Representational fungi poison rope has aflatoxin, ochratoxin, patulin, trichothecene family toxin, zearalenone, fumonisin, aspergillus versicolor rhzomorph, Fusarlum moniliforme element, citrinin etc.The human or animal takes in the food that is polluted by mycotoxin, or by sucking and skin contact mycotoxin can cause multiple toxicity symptom, to body even cause permanent lesion.Therefore, along with the development of detection method and analytical technology, it is found that mycotoxin almost extensively is present in all food and feed.So, the check and analysis of mycotoxin are especially paid attention to.
Mycotoxin is a small-molecule substance, and is extremely heat-resisting, and toxicity is not destroyed because of common heating, can cause the infringement of many organs, and has pathogenic effects at a specified future date.The more important thing is that the grain that is polluted by mycotoxin is normal in appearance, is difficult for being noted by people.Along with the progress of mycotoxin detection means, the 21 century research of mycotoxin will be one of heat subject.The existence of mycotoxin can threaten human health, causes social economy's loss, and therefore, the prevention of mycotoxin, detoxification, detoxifcation, the especially foundation of the detection method of fast high-sensitive degree seem very important.
The check and analysis method of mycotoxin generally has thin-layered chromatography (TCL), high performance liquid chromatography (HPLC), immunoassay etc., and the rapid analysis of modern mycotoxin mainly contains enzyme linked immunosorbent assay (ELISA) and immunoaffinity chromatography-fluorescence method.Abroad, the report of existing more mycotoxin detection.Yu etc. (2005) have set up Ochratoxin A (OTA) polyclonal antibody direct competitive ELISA method.Schneider etc. (2000) utilize chicken yolk antibody to set up the indirect competitive ELISA method of deoxynivalenol (DON) in the cereal.(2002) such as Bird CB add 1.0,3.0 in sample, during 5.0mg/kg fumonisins (FM), average recovery rate is respectively 120%, 100%, 90%.Xu etc. (1988) utilize direct ELISA method to measure cereal DON, and the recovery was 100%-102.1% when sample interpolation concentration was 10-1000ng/ml.Liu etc. (1985) have set up the polyclonal antibody indirect competitive ELISA method of zearalenone (ZEN) in detection cereal, wheat, the pig feed.Japan Kyowa Medex Co., Ltd. has invented and has been used for method and the reagent that the trichothecene mycotoxin detects, and has applied for patent in 2000.The numerous and confused mycotoxin ELISA detection kit of developing based on monoclonal or polyclonal antibody of European and American countries, and commercialization be widely used in agricultural product and food safety detection, but import price are higher.Domestic, Chu Qinghua etc. (2005) utilize the immune affinity column chromatography to purify fluorophotometric method and immune affinity column chromatography and purify high performance liquid chromatography and set up method for quick to aflatoxins, ochratoxin A and zearalenone in oil plant (soybean, rapeseed) and the edible vegetable oil.Huang Biao etc. (2006) adopt the time-resolved fluorescence technology to set up high-sensitive aflatoxin B1 (AFB1) indirect competition immunoassay (AFB-TRFIA).Sun passes and waits (1990) to adopt immunological technique to set up the national standard (GB/T14933-94) of detection mycotoxin.These detection methods all have the cycle long mostly, the process complexity, and shortcoming such as waste time and energy.
Owing to more, the newfound mycotoxin of the analog of most of mycotoxins constantly increases, mycotoxin pollutes ubiquity and harmfulness by reasons such as understanding progressively, the fast high-sensitive degree analytical approach of mycotoxin has also been proposed more urgent and higher requirement.At present, the difficult point of restriction mycotoxin detection research mainly is: the structural similarity thing is more, and cross reaction is general; Toxin is subject to the influence of the interfering material that other is difficult to remove in the extract etc. when detecting., detection difficult big, be difficult for characteristics such as prevention at current mycotoxin harmfulness, especially concerning commodity inspection and health and epidemic prevention department, more needing easy, quick, a sensitive method detects mycotoxin, its potential hazard is judged, to promote economic development, ensure human health.
Now a lot of fields are all utilizing screen printing technique to make the electrode of different use, and what disclose in the Chinese patent application number 85102225,99812560.1,00130620.0 and 02157282.8 is exactly this screen printing electrode.But the relevant report of the disposable mycotoxin detection of biological sensor-based system molecular engram film electrode of being made by screen printing technique that really is used for industrialization is not seen as yet.
Summary of the invention
The technical problems to be solved in the utility model has provided a kind of molecular engram film electrode that is used for the mycotoxin detection easy to detect, with low cost, easy to carry.
In order to solve the problems of the technologies described above, the utility model is realized by following measure: a kind of molecular engram film electrode that is used for the mycotoxin detection, comprise electrode base sheet, two electrode matrixs that printing or stickup are made by conductive material on the described electrode base sheet, it is characterized in that: one of described two electrode matrix are connected as a body by electrode connecting line and working electrode by connection terminal, and another electrode matrix is connected as a body by electrode connecting line with to electrode by connection terminal; Described electrode connecting line surface-coated one deck polycarbonate, be coated with responding layer on the described working electrode, described responding layer is made up of the molecularly imprinted polymer that is fixed on energy on the working electrode and mycotoxin generation special molecular identification biochemical reaction with surface modification technology.
In order to make detection more accurate, the utility model also comprises the contrast electrode that is fixed on the described electrode base sheet.
Working electrode described in the utility model also is coated with the carbon nano-tube that one deck is used for nano-synergistic.
Working electrode described in the utility model be shaped as quadrilateral, described being shaped as of electrode is centered around the working electrode outside
Shape.
The utility model also includes loam cake, and described loam cake covers the top of entire electrode, described contrast electrode, working electrode, electrode, connection terminal and partial electrode line is exposed to the outside.
The beneficial effects of the utility model:
1. owing to use screen printing electrode, that its electrode changes is easy, miniaturization, portable, can disposablely use.
2. responding layer is by being fixed on the working electrode with surface modification technology, by the proportioning adjustment of immobilization material, make the molecular engram film electrode that makes to environment temperature require not obviously, use under the room temperature to get final product.
3. the preparation method is simple, stable performance, and the good reproducibility of electrode is applicable to the practical application of mycotoxin detection of biological sensor industrialization.
4. because the application of screen printing technique reduces the technology cost of making electrode, be suitable for requirement inexpensive in the industrialization, this helps making disposable products, makes things convenient for the client to use.
5. screen printing technique is not subjected to the restriction of stock size and shape, and the characteristics of its dirigibility and practicality make it not be subjected to restrictions such as shape, size when making electrode, make the molecular engram film electrode product of different size, enrich product category.
6. because serigraphy can be carried out industrialized large-scale production, make that electrode can be mass customized on producing, it is not subjected to the restriction of enterprise's size, and big interchangeable manufacturing can be produced, and township and village enterprises, individual handicraft industry also can be produced.
The screen printing electrode specification little, in light weight, be easy to carry about with one, make things convenient for, practical.
8. be the biological sensing system of the molecular engram film electrode establishment of immobilization carrier fixed member imprinted polymer with the screen printing electrode, can realize quick online detection, can be controlled in detection time about 10min, detect and be limited to 0.1ng/g mycotoxin.
9. responding layer has added carbon nano-tube, utilizes its nano-synergistic effect, has significantly improved the being quick on the draw property and the accuracy of detection of prepared electrode, makes testing result more accurate.
10. be fixed with contrast electrode on the described electrode base sheet, make detection more accurate.
Description of drawings
Below in conjunction with the drawings and specific embodiments the utility model is described in further details.
Fig. 1 is the front elevational schematic of electrode base sheet.
Fig. 2 is the front elevational schematic of three electrode matrixs.
Fig. 3 is the front elevational schematic of responding layer.
Fig. 4 is the front elevational schematic of loam cake.
Fig. 5 is the front elevational schematic of the utility model molecular engram film electrode.
Fig. 6 is the front elevational schematic of removing the utility model molecular engram film electrode of loam cake.
Fig. 7 is the reaction principle figure of the utility model molecular engram film electrode.
1. connection terminals among the figure, 2. electrode connecting line, 3. contrast electrode, 4. working electrode, 5. pair electrode, 6. responding layer, 7. window, 8. loam cake, 9. electrode base sheet.
Embodiment
A kind of molecular engram film electrode that is used for the mycotoxin detection as shown in Figure 5, comprise electrode base sheet 9, two electrode matrixs that printing or stickup are made by conductive material on the described electrode base sheet 9, one of described two electrode matrix are connected as a body by electrode connecting line 2 and working electrode 4 by connection terminal 1, and another electrode matrix is connected as a body by electrode connecting line 2 with to electrode 5 by connection terminal 1; Described electrode connecting line 2 surface-coated one deck polycarbonate, be coated with responding layer on the described working electrode 4, described responding layer is made up of the molecularly imprinted polymer that is fixed on energy on the working electrode 4 and mycotoxin generation special molecular identification biochemical reaction with surface modification technology.
In order to make detection more accurate, the utility model also comprises the contrast electrode 3 that is fixed on the described electrode base sheet 9.
Working electrode 4 described in the utility model also is coated with the carbon nano-tube that one deck is used for nano-synergistic.
Shown in Fig. 2 .4 working electrode 4 described in the utility model be shaped as quadrilateral, described being shaped as of electrode 5 is centered around working electrode 4 outsides
Shape.
The utility model also includes loam cake 8 shown in Fig. 4 .5, and described loam cake 8 covers the top of entire electrode, described contrast electrode 3, working electrode 4, electrode 5, connection terminal 1 and partial electrode line 2 is exposed to the outside.
Responding layer is made up of the molecularly imprinted polymer that is fixed on energy on the working electrode 4 and mycotoxin generation special molecular identification biochemical reaction with surface modification technology as shown in Figure 3.
Fig. 1, Fig. 2, Fig. 3, Fig. 4 and Fig. 5 show the formation situation that the utility model of 1 working electrode molecular engram film electrode is arranged.Fig. 1 shows the electrode base sheet 9 of size for long 35mm, wide 12mm, thick 0.5mm; Working electrode 4 is the square sheets of 3 * 3mm, is connected as a single entity by electrode connecting line 2 and a connection terminal 1; Electrode 5 is long 4.5mm, wide 1mm, high 4.5mm's
The shape slice, thin piece, the one side is connected as a single entity by electrode connecting line 2 and another connection terminal 1; Responding layer 6 is a square sheets that equates with working electrode 9 areas; Loam cake 8 long 26mm, wide 12mm, thick 0.2mm.
During manufacturing be working electrode, platinum line as to electrode with the platinum electrode, saturated calomel is as the contrast electrode material, prints or is bonded in bonding agent on the assigned address of electrode base sheet 9 by spraying process with connection terminal 1, electrode connecting line 2, contrast electrode 3, working electrode 4 with to electrode 5.Fig. 5 represents that loam cake 8 is bonded in electrode base sheet 9, electrode connecting line 2, contrast electrode 3, working electrode 4 and on the electrode 5, forms a complete molecular engram film electrode that 1 square working electrode is arranged.
The preparation method that the utility model is used for the molecular engram film electrode of mycotoxin detection is:
The electrode base sheet of making at the organic insulation of being made by screen printing technique is on the carrier, conductive material print formed working electrode, contrast electrode with spraying process and to the matrix of electrode, apply one deck polycarbonate insulator on their the center section surface, responding layer in the formation of working electrode matrix end portion coating surface modification technique, promptly make molecular engram film electrode, the concrete preparation method of its responding layer is:
The first step is used 0.5 μ m and 0.3 μ mAl successively
2O
3Powder polishes platinum electrode, uses 50% HNO again
3Handle, fully wash 3 times with acetone and redistilled water respectively at last.
Second step, get the 5ul carbon nano-tube solution, evenly coat working electrode surface, dry under the room temperature.
In the 3rd step, it is to dissolve in 5% the solvent that molecularly imprinted polymer is joined concentration.Get the above-mentioned mixed liquor of 10 μ L and be added drop-wise to working electrode surface, be coated with film forming equably, at room temperature dry.
The 4th step is with the unnecessary molecularly imprinted polymer of buffer solution flush away.The molecular engram film electrode of making is placed 4 ℃ of following refrigerators, use behind the preservation 24h.
The percent concentration of used buffer solution is the weight/volume specific concentration in the said method, and used molecularly imprinted polymer is selected according to different detection molecules, and the prescription of used damping fluid is Na
2HPO
41.15 gram, KCl 0.2 gram, NaCl 8.0 grams, KH
2PO
40.2 the gram, constant volume in the volumetric flask of 1000mL, pH=7.4.
The utility model principle of work as shown in Figure 7:
Initiation reaction behind the reactants such as adding mycotoxin molecule, function monomer, crosslinking chemical makes its polymerization in solvent.Then utilize physics or chemical means that mycotoxin molecule wash-out from the polymkeric substance of preparation is come out, polymkeric substance inside has just formed the hole that shape and position of functional group and mycotoxin molecule are complementary, this hole has the specific identification and the binding ability of height to the mycotoxin molecule, when the test substance in the sample therewith the polymkeric substance specificity in conjunction with after, in conjunction with photoelectric conversion technique, light signal is transformed into electric signal, and the size of the electric current that produces according to electrode surface can be measured the content of mycotoxin.
Claims (5)
1. one kind is used for the molecular engram film electrode that mycotoxin detects, comprise electrode base sheet (9), described electrode base sheet (9) is gone up printing or is pasted two electrode matrixs being made by conductive material, it is characterized in that: one of described two electrode matrix are connected as a body by electrode connecting line (2) and working electrode (4) by connection terminal (1), and another electrode matrix is connected as a body by electrode connecting line (2) with to electrode (5) by connection terminal (1); Described electrode connecting line (2) surface-coated one deck polycarbonate, described working electrode is coated with responding layer on (4), and described responding layer is made up of the molecularly imprinted polymer that is fixed on energy on the working electrode (4) and mycotoxin generation special molecular identification biochemical reaction with surface modification technology.
2. according to the described molecular engram film electrode that is used for the mycotoxin detection of claim 1, it is characterized in that: also include the contrast electrode (3) that is fixed on the described electrode base sheet (9).
3. according to the described molecular engram film electrode that is used for the mycotoxin detection of claim 2, it is characterized in that: described working electrode (4) also is coated with the carbon nano-tube that one deck is used for nano-synergistic.
4. be used for the molecular engram film electrode that mycotoxin detects according to claim 1 is described, it is characterized in that: described working electrode (4) be shaped as quadrilateral, described being shaped as of electrode (5) is centered around working electrode (4) outside
Shape.
5. according to claim 1.2.3 or 4 any described molecular engram film electrodes that are used for the mycotoxin detection, it is characterized in that: also include loam cake (8), described loam cake (8) covers the top of entire electrode, described contrast electrode (3), working electrode (4), electrode (5), connection terminal (1) and partial electrode line (2) is exposed to the outside.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102250285A (en) * | 2010-05-19 | 2011-11-23 | 中国科学院大连化学物理研究所 | Semi-covalent molecularly imprinted polymer used for selectivity separating phenols, preparation method thereof, and application thereof |
| CN101532987B (en) * | 2009-04-11 | 2012-02-15 | 桂林工学院 | Method for measuring chlorotoluron content |
| CN102549424A (en) * | 2009-08-27 | 2012-07-04 | 全技术公司 | Synthetic mycotoxin adsorbents and methods of making and utilizing the same |
| CN102706927A (en) * | 2012-04-11 | 2012-10-03 | 桂林理工大学 | Method for measuring gibberellin in fruits by aid of molecularly imprinted electrochemically modified electrode |
| CN101556258B (en) * | 2009-04-03 | 2012-11-28 | 江苏工业学院 | Timolol maleate potentiometric chemical sensor and preparation method thereof |
| CN104316427A (en) * | 2014-11-05 | 2015-01-28 | 中国科学院苏州生物医学工程技术研究所 | Rapid detection sensor for tetrodotoxin |
| CN108872342A (en) * | 2018-08-23 | 2018-11-23 | 佛山科学技术学院 | A kind of electrochemistry molecular engram sensor |
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2008
- 2008-05-07 CN CNU2008200217460U patent/CN201184874Y/en not_active Expired - Fee Related
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101556258B (en) * | 2009-04-03 | 2012-11-28 | 江苏工业学院 | Timolol maleate potentiometric chemical sensor and preparation method thereof |
| CN101532987B (en) * | 2009-04-11 | 2012-02-15 | 桂林工学院 | Method for measuring chlorotoluron content |
| CN102549424A (en) * | 2009-08-27 | 2012-07-04 | 全技术公司 | Synthetic mycotoxin adsorbents and methods of making and utilizing the same |
| CN102549424B (en) * | 2009-08-27 | 2016-01-13 | 全技术公司 | The method of synthesis mycotoxin absorbant and preparation and the described synthesis mycotoxin absorbant of use |
| CN102250285A (en) * | 2010-05-19 | 2011-11-23 | 中国科学院大连化学物理研究所 | Semi-covalent molecularly imprinted polymer used for selectivity separating phenols, preparation method thereof, and application thereof |
| CN102706927A (en) * | 2012-04-11 | 2012-10-03 | 桂林理工大学 | Method for measuring gibberellin in fruits by aid of molecularly imprinted electrochemically modified electrode |
| CN104316427A (en) * | 2014-11-05 | 2015-01-28 | 中国科学院苏州生物医学工程技术研究所 | Rapid detection sensor for tetrodotoxin |
| CN108872342A (en) * | 2018-08-23 | 2018-11-23 | 佛山科学技术学院 | A kind of electrochemistry molecular engram sensor |
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Granted publication date: 20090121 Termination date: 20110507 |