CN201183796Y - Biomechanics cell cultivation instrument - Google Patents
Biomechanics cell cultivation instrument Download PDFInfo
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- CN201183796Y CN201183796Y CNU2008200554572U CN200820055457U CN201183796Y CN 201183796 Y CN201183796 Y CN 201183796Y CN U2008200554572 U CNU2008200554572 U CN U2008200554572U CN 200820055457 U CN200820055457 U CN 200820055457U CN 201183796 Y CN201183796 Y CN 201183796Y
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Abstract
The utility model provides a biomechanical cell planting apparatus which comprises a cell planting box that contains a fibre tissue, a towing structure that is contacted with the fiber tissue for towing the fibre tissue in the cell planting box, and a step motor for driving programs; the step motor is electrically connected with the towing structure and drives the towing structure. The biomechanical cell planting apparatus is convenient for studying how enchylema affects the cell biologically.
Description
Technical field
The utility model relates to a kind of biomechanics cell cultivation instrument, relates more specifically to a kind of skin flbroblast be carried out the tensile cell culture apparatus.
Background technology
Usually, prior biological mechanics cell cultivation instrument comprises following type:
(1), micropipette aspiration method cell cultivation instrument: the magnetism of utilizing pressure difference to produce is measured the elasticity of cytolemma.Measure and suck the required negative pressure value of unit length cell, measure the cell length that sucks under the constant negative pressure value, measure and suck the required negative pressure value of whole cell.But its weak point is that it is suitable for individual cells is measured, but can't study cell aggregate (for example dermal tissue).
(2), fluid shearing force method cell cultivation instrument: flowing culturing cell under the field of force, analyzing its form then, physiological indexes.But this method only is suitable for vascular endothelial cell is analyzed.
(3), elastica pipe expansion deceleration loading device: with cell cultures on the silica gel elastica, it there is the one side of cell outwards to be close to be wrapped in to execute carries the circular cylinder that device outside head-to-tail forms a closure, place in the culture tank, this circular cylinder can make to be close to executes the elastica extension of carrying on the device, thereby the cell that is grown on the elastica is realized stretching, and then make elastica reinstatement attitude.So go round and begin again, realize that the periodicity mechanicalness of pair cell stretches.Characteristics: can imitate blood vessel vascular endothelial cell is carried out observation analysis, how pair cell carries out biological impact but it can't study cell matrix (ECM).。
(4), twin shaft mechanics strain system (Flexercell): the cell cultures trench bottom is fixing elastica, cell cultures is on elastica, the elastica bottom links to each other with an extraneous vacuum pump or fluid, utilizes vacuum pressure or fluid flow rate to change and makes elastica do expanding type motion up and down.The physiological environment of similar lung is fit to lung fibroblast is carried out analysis and observation.Also can carry out special processing, become two closed chamber,, it be expanded, then can realize horizontal stretch, be used for pair cell and carry out the analysis of mechanicalness mechanics influence central elastica to implementing negative pressure in the chamber to elastica two.How pair cell carries out biological impact but this device can't be studied cell matrix (ECM).
(5), magnetic bead torsional technique: will magnetize microballon in conjunction with certain peptide species, carrying out specificity with cell surface again combines, then this cell is put into another and direction and the vertical magnetic field of former field direction, measured the size and the direction of magnetic force by the variation of measuring magnetic force.This method is used to measure the rigidity of cell.How pair cell carries out biological impact but this device can't be studied cell matrix (ECM).
(6), hydrostatic platen press: under hydraulic pressure, survey culturing cell, utilize the hydraulic pressure pair cell to produce pressure.Be used to study the influence of special environment pair cell.How pair cell carries out biological impact but this device can't be studied cell matrix (ECM).
(7), sound stimulation method: utilize the sound stimulation cell surface.Observe the influence of particular surroundings pair cell.How pair cell carries out biological impact but this device can't be studied cell matrix (ECM).
(8), optical tweezer: utilize the laser light pincers that the polymer bead of processing is combined with cell surface, mobile then laser is pulled out a filament that is made of cytolemma by bead, and measures the pulling force that filament produced, thereby measures the tensile elasticity of cytolemma.Be fit to individual cells is studied.How pair cell carries out biological impact but this device can't be studied cell matrix (ECM).
(9), centrifuge field method: culturing cell and bacterium in centrifuge field, observation centrifugal force pair cell pattern and physiological influence.Be suitable for observing the influence of particular surroundings pair cell.How pair cell carries out biological impact but this device can't be studied cell matrix (ECM).
(10), atomic force microscopy: with the probe irritation cell of atomic force microscope, the morphology change of observation of cell and physiologic response.Be suitable for individual cells is studied.How pair cell carries out biological impact but this device can't be studied cell matrix (ECM).
(11), gravity method: culturing cell in space or the super weightlessness of artificial, how pair cell carries out biological impact but this device is not suitable for studying cell matrix (ECM).
(12), shaking table method: Tissue Culture Dish is placed on culturing cell in the shaking table, and this method is simple and convenient, but is difficult for quantitative examination.
In sum, this area lacks a kind of cell cultivation apparatus that is applicable to the influence of research cell matrix (ECM) pair cell biomechanics, for this reason, this area press for a kind of can study easily cell matrix (ECM) how the pair cell biomechanics of carrying out biological impact cultivate instrument.
The utility model content
The purpose of this utility model is to obtain a kind of biomechanics of studying the influence of cell matrix ECM pair cell biomechanics and cultivates instrument.
An aspect of the present utility model provides a kind of biomechanics cell cultivation instrument, and described cultivation instrument comprises:
-cell culture case holds fibrous tissue in the described cell culture case,
-hitch structure, described hitch structure is connected with described fibrous tissue contact, and the fibrous tissue that is used for pair cell cultivation box draws;
-being provided with the stepper-motor of driver, described stepper-motor is connected with described hitch structure electricity and drives described hitch structure.
In an embodiment of the present utility model, described fibrous tissue is an emulation corium.
In an embodiment of the present utility model, described hitch structure is connected with the one or both ends contact of described fibrous tissue,
Wherein, described hitch structure is a suspending tractive structure, and it comprises:
-suspending pull wire, described pull wire one end is connected with described fibrous tissue, and the other end is connected with the roller bearing of described stepper-motor, thereby makes the roller bearing running of described stepper-motor drive the passive stretching of described fibrous tissue or unfold motion;
The upstream of-described pull wire is provided with stationary installation, is used for fixing described fibrous tissue.
In an embodiment of the present utility model, described stationary installation comprises first fixed plate and second fixed plate,
-described first fixed plate comprises the upper and lower, forms first slit between the described upper and lower) so that an end of fibrous tissue therefrom passes,
The downstream of-the first fixed plate is provided with second fixed plate, and described second fixed plate comprises the upper and lower, forms second slit between the described upper and lower, so that the other end of fibrous tissue therefrom passes, and is connected with described pull wire.
In an embodiment of the present utility model, cell cultivation instrument comprises: described hitch structure is connected with the one or both ends contact of described fibrous tissue,
Wherein, described hitch structure is a run-in index tractive structure, and it comprises:
-horizontally tracting line, described pull wire one end is connected with described fibrous tissue, and the other end is connected with the roller bearing of described stepper-motor, thereby makes described stepper-motor roller bearing running drive the passive stretching of described fibrous tissue or unfold motion;
The upstream of-described pull wire is provided with stationary installation, is used for fixing described fibrous tissue.
In an embodiment of the present utility model, described stationary installation comprises first fixed plate and second fixed plate,
-described first fixed plate comprises the upper and lower, forms first slit between the described upper and lower, so that an end of fibrous tissue therefrom passes,
The downstream of-the first fixed plate is provided with second fixed plate, and described second fixed plate comprises the upper and lower, forms second slit between the described upper and lower, so that the other end of fibrous tissue therefrom passes, and is connected with described pull wire.
In an embodiment of the present utility model, described hitch structure is a vacuum tractive structure, and it comprises:
-traction vacuum pump, described vacuum pump is provided with pump orifice and is connected with described cell culture case, and another pump orifice is connected with described stepper-motor;
In-described the cell culture case stationary installation is set, is used for fixing described fibrous tissue.
In an embodiment of the present utility model, described stationary installation comprises first fixed plate and second fixed plate,
-described first fixed plate comprises the upper and lower, forms first slit between the described upper and lower, so that an end of fibrous tissue therefrom passes,
The downstream of-the first fixed plate is provided with second fixed plate, and described second fixed plate comprises the upper and lower, forms second slit between the described upper and lower, so that the other end of fibrous tissue therefrom passes, and is connected with described pull wire.
In an embodiment of the present utility model, the downstream of described cell cultivation instrument also is provided with the mechanics determinator.
Particularly, described mechanics determinator comprises swept resistance, wherein said cell cultivation instrument is by the stepper-motor tractive, a downstream swept resistance in parallel, by swept resistance, with one group of amplifirer signal is amplified then, change by digital to analog converter again, can measure the power that tractive produces, also the tension force situation that can produce the cell that is grown on the film is measured.
In an embodiment of the present utility model, the downstream of described cultivation instrument also is provided with mechanics sensor.
Particularly, the setting of described mechanics sensor is: by computer control, import required power, by digital-to-analog conversion, become the electrical signal of required power, act on stepper-motor then, bionic dermis is applied biomechanics to stimulate.
Advantage of the present utility model is:
1, not only can study the biomechanics influence of the various composition pair cells of cell matrix ECM, also can study cell matrix structure (two dimension or three-dimensional) pair cell biomechanics influence
2, can carry out mechanics biological nature (Young's modulus) and carry out quantitative Journal of Sex Research tissue such as different times scar tissue, tendon, nerve, cartilage, decalcification bone.
3, also can carry out biomechanical analysis and evaluation to organizational project organ such as artificial skin, tendon, cartilage etc.
Description of drawings
Fig. 1 is the schematic top plan view of the cell culture case of a specific embodiment of the present utility model;
Fig. 2 is the side schematic view of the cell culture case part of Fig. 1, and wherein this structure transmission is to drive the cell cultures film by cantilever, carries out the suspending tractive, and the other end links to each other with motor;
Fig. 3 is cell culture case vertical view 3a and cell transmission synoptic diagram 3b.This structure is for to carry out parallel tractive by parallel tractive pair cell culture membrane.
Fig. 4 is cell culture case and transmission synoptic diagram: this structure links to each other by a vacuum pump, contracts by selecting in the different cell boxes the removable plate in side to relax, and the physiological mechanics tractive is simulated in substrate to the cultivation box.
Fig. 5 is the structural representation (comprising film and bridge pier) of fibrous tissue.A is that the plane is seen for three-dimensional sight, B, and redness is a bridge pier, is coated with the cellular affinity protein molecular, and blue look is the acellular affinity molecule.
Fig. 6 is an embodiment that the mechanics determinator is set of the present utility model.
Fig. 7 is an embodiment that mechanics sensor is set of the present utility model.
Embodiment
The utility model designer is through extensive and deep research, by improving preparation technology, obtained a kind of cell cultivation instrument, and be surprised to find that this device is excellent especially to the biomechanics influence of research extracellular matrix pair cell, so be particularly suitable as the biomechanics influence of research two dimension or three-dimensional cell epimatrix ECM structure pair cell.Finished the utility model on this basis.Principle of the present utility model is to adopt periodically elongate substrate method: utilizes the adherent property of cell when cultivating, cell cultivated in extendible substrate, and periodic then elongate substrate, thus pair cell produces periodic drawing force.
Described a kind of biomechanics cell cultivation instrument of the present utility model, described cultivation instrument comprises:
-cell culture case 1 holds fibrous tissue 11 in the described cell culture case 1,
-hitch structure 2, described hitch structure 2 is connected with described fibrous tissue 11 contacts, and the fibrous tissue 11 that is used for pair cell cultivation box 1 draws;
-being provided with the stepper-motor 3 of driver, described stepper-motor 3 is connected with described hitch structure 2 electricity and drives described hitch structure 2.
Particularly, in suspending tractive structure, comprise: suspending pull wire 132, described pull wire 132 1 ends are connected with described fibrous tissue 11, the other end is connected with the roller bearing of described stepper-motor 3, thereby makes the roller bearing running of described stepper-motor 3 drive the passive stretching of described fibrous tissue 11 or unfold motion; The upstream of described pull wire 132 is provided with stationary installation, is used for fixing described fibrous tissue 11.Described stationary installation comprises first fixed plate 131 and second fixed plate 133, described first fixed plate 131 comprises top 131a and bottom 131b, form the first slit 131c between described top 131a and the bottom 131b, so that an end of fibrous tissue 11 therefrom passes, the downstream of first fixed plate 131 is provided with second fixed plate 133, described second fixed plate 133 comprises top 133a and bottom 133b, form the second slit 133c between described top (133a) and the bottom 133b, so that the other end of fibrous tissue 11 therefrom passes, and be connected with described pull wire 132.
Particularly, in run-in index tractive structure, comprise: horizontally tracting line 132, described pull wire 132 1 ends are connected with described fibrous tissue 11, the other end is connected with the roller bearing of described stepper-motor 3, thereby makes the roller bearing running of described stepper-motor 3 drive the passive stretching of described fibrous tissue 11 or unfold motion; The upstream of described pull wire 132 is provided with stationary installation, is used for fixing described fibrous tissue 11.Described stationary installation comprises first fixed plate 131 and second fixed plate 133, described first fixed plate 131 comprises top 131a and bottom 131b, form the first slit 131c between described top 131a and the bottom 131b, so that an end of fibrous tissue 11 therefrom passes, the downstream of first fixed plate 131 is provided with second fixed plate 133, described second fixed plate 133 comprises top 133a and bottom 133b, form the second slit 133c between described top 133a and the bottom 133b, so that the other end of fibrous tissue 11 therefrom passes, and be connected with described pull wire 132.
Particularly, be that it comprises in the vacuum tractive structure: traction vacuum pump 134, described vacuum pump 134 is provided with pump orifice and is connected with described cell culture case 1, and another pump orifice is connected with described stepper-motor 3; In the described cell culture case 1 stationary installation is set, is used for fixing described fibrous tissue 11.Described stationary installation comprises first fixed plate 131 and second fixed plate 133, described first fixed plate 131 comprises top 131a and bottom 131b, form the first slit 131c between described top 131a and the bottom 131b, so that an end of fibrous tissue 11 therefrom passes, the downstream of first fixed plate 131 is provided with second fixed plate 133, described second fixed plate 133 comprises top 133a and bottom 133b, form the second slit 133c between described top 133a and the bottom 133b, so that the other end of fibrous tissue 11 therefrom passes, and be connected with described pull wire 132.
The downstream of described cell cultivation instrument can also be provided with traditional mechanics determinator.
Particularly, described mechanics determinator comprises swept resistance, wherein said cell cultivation instrument is by the stepper-motor tractive, a downstream swept resistance in parallel, by swept resistance, with one group of amplifirer signal is amplified then, change by digital to analog converter again, can measure the power that tractive produces, also the tension force situation that can produce the cell that is grown on the film is measured.
The downstream of described cell cultivation instrument can also be provided with traditional mechanics sensor.
Particularly, the setting of described mechanics sensor is: by computer control, import required power, by digital-to-analog conversion, become the electrical signal of required power, act on stepper-motor then, bionic dermis is applied biomechanics to stimulate.
Other aspects of the present utility model are because the disclosure of this paper is conspicuous to those skilled in the art.
Below in conjunction with specific embodiment, further illustrate the utility model.Should be understood that these embodiment only to be used to the utility model is described and be not used in the restriction scope of the present utility model.The experimental technique of unreceipted actual conditions in the following example, usually according to normal condition, or the condition of advising according to manufacturer.Ratio and per-cent are based on weight, unless stated otherwise.
Unless otherwise defined or explanation, same meanings of being familiar with of all specialties used herein and scientific words and those skilled in the art.Any in addition method similar or impartial to described content and material all can be applicable in the utility model method.
Embodiment
As depicted in figs. 1 and 2, a kind of biomechanics cell cultivation instrument, described cultivation instrument comprises :-cell culture case 1, described cell culture case 1 forms fibrous tissue 11,-hitch structure 2, described hitch structure 2 is connected with described fibrous tissue 11 contacts, and the fibrous tissue 11 that is used for pair cell cultivation box 1 draws;-being provided with the stepper-motor 3 of driver, described stepper-motor 3 is connected with described hitch structure 2 electricity and drives described hitch structure 2.
Described hitch structure 2 is a suspending tractive structure, and it comprises:
-suspension traction line 132, described pull wire 132 1 ends are connected with described fibrous tissue 11, and the other end is connected with the roller bearing of described stepper-motor 3, thereby makes the roller bearing running of described stepper-motor 3 drive the passive stretching of described fibrous tissue 11 or unfold motion;
The upstream of-described pull wire 132 is provided with stationary installation, is used for fixing described fibrous tissue 11.
Described stationary installation comprises first fixed plate 131 and second fixed plate 133,
-described first fixed plate 131 comprises top 131a and bottom 131b, forms the first slit 131c between described top 131a and the bottom 131b, so that an end of fibrous tissue 11 therefrom passes,
The downstream of-the first fixed plate 131 is provided with second fixed plate 133, described second fixed plate 133 comprises top 133a and bottom 133b, form the second slit 133c between described top 133a and the bottom 133b,, and be connected with described pull wire 132 so that the other end of fibrous tissue 11 therefrom passes.
Above-mentioned biomechanics cell cultivation instrument is like this running: in cell culture case 1, four of fixed plates ( comprise 131a, 131b, 133a, 133b) relative in twos, therebetween fibrous tissue 11 (bionic dermis), screw tightening.
Described cell culture case 1 is located on the cell culture case substrate 1a of base.One 133 of fixed plate is fixed on the cell culture case substrate 1a, plays fixed action.One 131 of fixed plate links to each other with fixed plate slide shaft 135, slide shaft 135 is fixed in the chute 136, have a coupling device 2a to be connected with as illustrated in fig. 1 and 2 tractive structure 2 (suspension system) on this fixed plate, tractive structure 2 is connected by three groups of pulleys, is connected at last on the motor drive shaft 3b.This transmission shaft 3b is connected on the stepper-motor 3 (two motors are formed), and motor is driven by drive unit 3a.Under drive unit 3 effects, stepper-motor 3 rotates and drives transmission shaft 3b rotation.The pulley that drives suspension system 2 then rotates, and makes to drive one 131 reciprocating in fixed plate chute 136 of fixed plate the living corium of walking back and forth and carry out the diastole campaign.
This kind design need not cultivating box boring, and in cultivating box at the bottom of the tractive side box corresponding position make chute, drawing plate places chute, when tractive, guarantees tractive along the chute translation, moves up and down and produce.Outside cultivating box, use pulley gear that the suspending pulling device is delivered on the stepper-motor hanging stick.The transmission of assurance power.
In a specific embodiment of the present invention, can also adopt software controlled machine that driving device for step-by-step is controlled.This device connects by the cable information line, one end is linked on the driving device for step-by-step, and an end links to each other with computer, by changing different parameters, size as power, rotating speed, the time of commentaries on classics, tractive and the period frequency that loosens, control by computer, various power are exported, observed under the various mechanical conditions, the biology of cell changes.
Above-mentioned fibrous tissue 11 is shown in Fig. 5 a and 5b, grow for cell, use a high-molecular biologic compatible material, by simulating different physiology, skin Young's modulus under the pathological state, produce different simulated skin films, then by little impression technique, print different bridge piers, above by the molecule self-assembly, utilize intermolecular hydrophilic, hydrophobic, or be with different electric positive and negative charges, or utilize intermolecular strong interaction power, or weak interaction force, or interact between the different chemical groups, various iuntercellular affinity molecule of the portion that is coated with on film and non-affinity molecule make this film pair cell have the selective adsorption effect.
Shown in Fig. 3 a, 3b, described hitch structure 2 is a run-in index tractive structure, and it comprises:
-pull wire 132, described pull wire 132 1 ends are connected with described fibrous tissue 11, and the other end is connected with the roller bearing of described stepper-motor 3, thereby makes the roller bearing running of described stepper-motor 3 drive the passive stretching of described fibrous tissue 11 or unfold motion;
The upstream of-described pull wire 132 is provided with stationary installation, is used for fixing described fibrous tissue 11.
As shown in Figure 4, described hitch structure 2 is a vacuum tractive structure, and it comprises:
-traction vacuum pump 134, described vacuum pump 134 is provided with pump orifice and is connected with described cell culture case 1, and another pump orifice is connected with described stepper-motor 3;
In-described the cell culture case 1 stationary installation is set, is used for fixing described fibrous tissue 11.
In an embodiment, adopt a software controlled machine (not shown) that stepper-motor is controlled: vacuum pump 134 is connected with computer, relevant parameter is set, by a series of experiments the negative pressure that vacuum pump 134 produces is linked to each other with the corresponding mechanics parameter earlier in advance, then in actually operating, required mechanics parameter is set, negative pressure speed, negative pressure and the time cycle of going negative pressure, operation in a day is several inferior, experimentize, observation of cell to the biology of generation change.
As shown in Figure 6, described cell cultivation instrument also comprises mechanics determinator 4.Described mechanics determinator 4 comprises a swept resistance, by stepper-motor 3 tractives, a swept resistance in parallel, pass through swept resistance, with one group of amplifirer 4a signal is amplified then, by the digital to analog converter conversion, can measure the power that tractive produces again, also the tension force situation that can produce the cell that is grown on the film is measured.
As shown in Figure 7, described cultivation instrument comprises mechanics sensor 5.By computer 5a control, import required power, by digiverter 5b, become the electrical signal of required power, act on stepper-motor 3 then, bionic dermis is applied biomechanics to stimulate.
During use, required mechanics sensor 5, can to given concrete mechanical index export, signal is passed to stepper-motor, drives.
All quote in this application as a reference at all documents that the utility model is mentioned, just quoted as a reference separately as each piece document.Should be understood that in addition those skilled in the art can make various changes or modifications the utility model after having read above-mentioned teachings of the present utility model, these equivalent form of values fall within the application's appended claims institute restricted portion equally.
Claims (10)
1, a kind of biomechanics cell cultivation instrument is characterized in that, described cultivation instrument comprises:
-cell culture case (1) holds fibrous tissue (11) in the described cell culture case (1),
-hitch structure (2), described hitch structure (2) is connected with described fibrous tissue (11) contact, and the fibrous tissue (11) that is used for pair cell cultivation box (1) draws;
-being provided with the stepper-motor (3) of driver, described stepper-motor (3) is connected with described hitch structure (2) electricity and drives described hitch structure (2).
2, cell cultivation instrument as claimed in claim 1 is characterized in that, described fibrous tissue (11) is an emulation corium.
3, cell cultivation instrument as claimed in claim 1 is characterized in that, described hitch structure (2) is connected with the one or both ends contact of described fibrous tissue (11),
Wherein, described hitch structure (2) is a suspending tractive structure, and it comprises:
-suspending pull wire (132), described pull wire (132) one ends are connected with described fibrous tissue (11), the other end is connected with the roller bearing of described stepper-motor (3), thereby makes the roller bearing running of described stepper-motor (3) drive the passive stretching of described fibrous tissue (11) or unfold motion;
The upstream of-described pull wire (132) is provided with stationary installation, is used for fixing described fibrous tissue (11).
4, cell cultivation instrument as claimed in claim 3 is characterized in that, described stationary installation comprises first fixed plate (131) and second fixed plate (133),
-described first fixed plate (131) comprises top (131a) and bottom (131b), forms first slit (131c) between described top (131a) and bottom (131b), so that an end of fibrous tissue (11) therefrom passes,
The downstream of-the first fixed plate (131) is provided with second fixed plate (133), described second fixed plate (133) comprises top (133a) and bottom (133b), form second slit (133c) between described top (133a) and bottom (133b), so that the other end of fibrous tissue (11) therefrom passes, and be connected with described pull wire (132).
5, cell cultivation instrument as claimed in claim 1 is characterized in that, it comprises: described hitch structure (2) is connected with the one or both ends contact of described fibrous tissue (11),
Wherein, described hitch structure (2) is a run-in index tractive structure, and it comprises:
-horizontally tracting line (132), described pull wire (132) one ends are connected with described fibrous tissue (11), the other end is connected with the roller bearing of described stepper-motor (3), thereby makes the roller bearing running of described stepper-motor (3) drive the passive stretching of described fibrous tissue (11) or unfold motion;
The upstream of-described pull wire (132) is provided with stationary installation, is used for fixing described fibrous tissue (11).
6, cell cultivation instrument as claimed in claim 5 is characterized in that, described stationary installation comprises first fixed plate (131) and second fixed plate (133),
-described first fixed plate (131) comprises top (131a) and bottom (131b), forms first slit (131c) between described top (131a) and bottom (131b), so that an end of fibrous tissue (11) therefrom passes,
The downstream of-the first fixed plate (131) is provided with second fixed plate (133), described second fixed plate (133) comprises top (133a) and bottom (133b), form second slit (133c) between described top (133a) and bottom (133b), so that the other end of fibrous tissue (11) therefrom passes, and be connected with described pull wire (132).
7, cell cultivation instrument as claimed in claim 1 is characterized in that,
Described hitch structure (2) is a vacuum tractive structure, and it comprises:
-traction vacuum pump (134), described vacuum pump (134) is provided with pump orifice and is connected with described cell culture case (1), and another pump orifice is connected with described stepper-motor (3);
In-described the cell culture case (1) stationary installation is set, is used for fixing described fibrous tissue (11).
8, cell cultivation instrument as claimed in claim 7 is characterized in that, described stationary installation comprises first fixed plate (131) and second fixed plate (133),
-described first fixed plate (131) comprises top (131a) and bottom (131b), forms first slit (131c) between described top (131a) and bottom (131b), so that an end of fibrous tissue (11) therefrom passes,
The downstream of-the first fixed plate (131) is provided with second fixed plate (133), described second fixed plate (133) comprises top (133a) and bottom (133b), form second slit (133c) between described top (133a) and bottom (133b), so that the other end of fibrous tissue (11) therefrom passes, and be connected with described pull wire (132).
9, cell cultivation instrument as claimed in claim 1 is characterized in that, the downstream of described cell cultivation instrument also is provided with mechanics determinator (4).
10, cell cultivation instrument as claimed in claim 1 is characterized in that, the downstream of described cell cultivation instrument also is provided with mechanics sensor (5).
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| CNU2008200554572U CN201183796Y (en) | 2008-02-04 | 2008-02-04 | Biomechanics cell cultivation instrument |
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| CNU2008200554572U CN201183796Y (en) | 2008-02-04 | 2008-02-04 | Biomechanics cell cultivation instrument |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101497861A (en) * | 2009-02-26 | 2009-08-05 | 中国人民解放军第三军医大学第一附属医院 | Tractor for circulating draw of anchorage-dependent cell |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101497861A (en) * | 2009-02-26 | 2009-08-05 | 中国人民解放军第三军医大学第一附属医院 | Tractor for circulating draw of anchorage-dependent cell |
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