CN201149563Y - Constant temperature staining bench for biological tissue slice - Google Patents
Constant temperature staining bench for biological tissue slice Download PDFInfo
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- CN201149563Y CN201149563Y CNU2007200975454U CN200720097545U CN201149563Y CN 201149563 Y CN201149563 Y CN 201149563Y CN U2007200975454 U CNU2007200975454 U CN U2007200975454U CN 200720097545 U CN200720097545 U CN 200720097545U CN 201149563 Y CN201149563 Y CN 201149563Y
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Abstract
The utility model provides a constant temperature staining platform for biological tissue sections belonging to the medical experiment instrument. The platform comprises a water bath box, staining jars with covers and an electric control box, wherein, the staining jars with covers containing different reagents are soaked in the water bath box full of water. An electric heating device in the water bath box is controlled by a temperature control circuit, resulting in keeping the water temperature constant in the box so that reagents in the staining jars are kept with the constant temperature by conduction. The staining platform is used for HE staining for tissue sections and ensures the constant temperature of reagents in different staining jars so as to meet staining chemical reaction conditions, thereby improving and stabilizing the effect of staining sections, and enhancing the diagnostic accuracy.
Description
Technical field
The utility model belongs to the medical experiment instrument field, is a kind of biological tissue section constant temperature dyeing platform, can be applicable to the routine techniques equipment of medical pathologies professional association section HE dyeing.
Background technology
The histotomy that is used for pathological diagnosis is the pathology technique laboratory with Pathologic specimen draw materials, fix, a series of program continuous workings such as dehydration, waxdip, paraffin embedding, microtome section, the roasting wax dewaxing of section, section statining, section sealing finish.The HE that wherein cuts into slices dyeing is that the staining jar with cover that will fill different reagent is arranged on the experiment table in certain sequence, requires the row dyeing flow according to specialty, makes to organize painted so that the medical pathology diagnosis of microscopically in the section.Generally continue to use atmospheric pressure state in the specialty, ambient temperature condition (hand dyeing, existing goods type automatic staining machine dye all like this).
It is inconsistent because ambient temperature is subjected to environment temperature, seasonal temperature variable effect, in every batch of long-term effect of dye liquor was used in the phase, can cause the relatively-stationary section statining effect of workflow heterogeneity, empirical adjustment dyeing time often can not be corrected or can not in time correct.
Summary of the invention
The purpose of this utility model is to overcome the deficiency of existing experimental technique, provides a kind of and can ensure that the temperature of reagent in the staining jar is constant, satisfies chemical reaction condition, improves, stablizes the section statining effect, the routine techniques of shortening working hours equipment.
The utility model solves its technical matters and is achieved through the following technical solutions:
The biological tissue section constant temperature dyeing platform that constitutes by water bath, staining jar with cover, electric cabinet, its technical scheme is: the staining jar with cover that respectively fills different reagent is embedded in the water bath, be soaked in the water, electric heater unit in the water bath is controlled by temperature-control circuit, make the water termostat in the water bath, conduction makes reagent constant temperature in the staining jar.
1. make a water bath with chemical grade hard PVC sheet material (thickness 10mm) welding with certain heat-insulating property, the water-bath case lid is removable piece installing, is fastened on the water bath with stainless steel bolt (M5 * 20).Water bath has covered 16 specification circular holes of two row side by side, is used to embed 16 high-quality stainless steel staining jars, with the sealing of " O " type rubber seal ring, prevents that water vapor is overflowed in the water bath.The dyeing cylinder cap is made with the high-quality stainless steel material, and it tightens the intrinsic button of carrying, and it is pasted with the fluorine-containing rubber gasket that can tolerate organic solvent down, has certain mass to be used for weight and covers completely, avoids reagent volatilization in the cylinder.
2. water bath one side wall surface bottom position perforate is two, inside respectively fastening single-ended electric heating tube (power 500w) and the temperature sensor of being equipped with.Its lead-in wire is formed control circuit jointly with discharge cock, temperature controller, relay in the homonymy panel box, and water temperature is controlled in 37 ℃ or 45 ℃ of (this temperature meets the medical experiment requirement, is temperature commonly used) temperature constant states in the water bath.One of the outer water bath sidewall bottom position perforate of panel box, inside fastening stainless steel (or copper) water valve is used for the use of refreshing the water periodically in the water bath.
The beneficial effects of the utility model are:
Utilize as above technical equipment, can ensure that the temperature of reagent in each staining jar is constant, satisfy the dyeing chemistry reaction conditions, improve, stablize the section statining effect, help medical diagnosis.This biological tissue section constant temperature dyeing platform is a kind of laboratory configuration device of suitable especially medical pathologies histotomy HE dyeing.
Description of drawings
Fig. 1 is a front view of the present utility model
Fig. 2 is the front schematic view of Fig. 1
Fig. 3 is the inner structure synoptic diagram of Fig. 1
Embodiment
The utility model is described in further detail below by instantiation, and following example is descriptive, is not determinate, can not limit protection domain of the present utility model with this.
A kind of biological tissue section constant temperature dyeing platform, constitute by water bath, staining jar with cover, electric cabinet, its design feature is: make a water bath 1. with chemical grade hard PVC sheet material (thickness 10mm) welding with certain heat-insulating property 1., the water-bath case lid is removable piece installing, is fastened on the water bath with some stainless steel bolts (M5 * 20).Water bath has covered 16 specification circular holes of two row side by side, is used to embed 16 high-quality stainless steel staining jars 2., with the sealing of " O " type rubber seal ring, prevents that water vapor is overflowed in the water bath.3. the dyeing cylinder cap is made with the high-quality stainless steel material, and 4. it tightens intrinsicly carries button, and it is pasted with the fluorine-containing rubber gasket that can tolerate organic solvent down, has certain mass (1kg) to be used for weight and covers completely, avoids that reagent volatilizees in the cylinder.2. water bath one side wall surface bottom position perforate is two, inwardly fastening respectively be equipped with single-ended electric heating tube (power 500w) 5. with temperature sensor 6..Its lead-in wire is formed control circuit jointly with discharge cock, temperature controller, the relay of homonymy panel box in 7., and water temperature is controlled in 37 ℃ or 45 ℃ of temperature constant states in the water bath.8. one of the outer water bath sidewall bottom position perforate of panel box, inside fastening stainless steel (or copper) water valve are used for changing in the regular water bath water and use.
Principle of work of the present utility model is:
The HE of histopathologic slide dyeing is a quite complicated course of reaction, and thinking at present in the specialty existing chemical action in its process has physical action again.Widespread usage standard form staining reagent in specialty, the dying operation under the normal temperature and pressure conditions.Think through our feasibility study: 1. the constant realization that helps stable Color of temperature conditions; 2. according in the inorganic chemistry---the principle of " temperature is the key factor that influences chemical reaction, and general chemical reaction speeds up with the rising of temperature ", suitably improve temperature of reagent, can satisfy the dyeing chemistry reaction conditions, improve Color; 3. under higher temperature conditions, the dyeing group in the dyeing liquor (chromophore and auxochrome) can better be dispersed into micromolecule and ionic condition, and can absorb enough energy and become better activated molecule, improves the chemical reaction rate of dyeing.
Based on as above principle, conjunctive tissue section HE dyeing routine work flow process, we have developed biological tissue section constant temperature dyeing (platform) equipment that is used for the HE of histopathologic slide dyeing, purpose is the series in the routine work flow process is filled the staining jar of different reagent, heated at constant temperature performance by this equipment, make the temperature of reagent in the staining jar constant, use the reagent of constant temperature to be used for tissue section strain, can satisfy the dyeing chemistry reaction conditions, improve, stablize the section statining effect, improve the accuracy of pathological diagnosis.
Claims (1)
1. biological tissue section constant temperature dyeing platform, constitute by water bath, staining jar with cover, electric cabinet, it is characterized in that: the staining jar with cover that respectively fills different reagent is embedded in the water bath, be soaked in the water, electric heater unit in the water bath is controlled by temperature-control circuit, make the water termostat in the water bath, conduction makes reagent constant temperature in the staining jar.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2007200975454U CN201149563Y (en) | 2007-11-07 | 2007-11-07 | Constant temperature staining bench for biological tissue slice |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2007200975454U CN201149563Y (en) | 2007-11-07 | 2007-11-07 | Constant temperature staining bench for biological tissue slice |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201149563Y true CN201149563Y (en) | 2008-11-12 |
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| Application Number | Title | Priority Date | Filing Date |
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| CNU2007200975454U Expired - Fee Related CN201149563Y (en) | 2007-11-07 | 2007-11-07 | Constant temperature staining bench for biological tissue slice |
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| CN (1) | CN201149563Y (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104502170A (en) * | 2014-12-24 | 2015-04-08 | 中国人民解放军总医院 | Immobilization device and immobilization method for pathological tissues |
| CN105401367A (en) * | 2015-11-15 | 2016-03-16 | 潘福健 | Water-bath heating type textile dye vat |
| CN105980827A (en) * | 2013-12-13 | 2016-09-28 | 文塔纳医疗系统公司 | Thermal management in the context of automated histological processing of biological specimens and associated technology |
| CN108760446A (en) * | 2018-05-18 | 2018-11-06 | 北京林业大学 | A kind of one body swimming bath for arbuscular mycorrhiza trypan blue staining |
| US10416176B2 (en) | 2013-12-13 | 2019-09-17 | Ventana Medical Systems, Inc. | Staining reagents and other liquids for histological processing of biological specimens and associated technology |
| CN110389220A (en) * | 2019-08-28 | 2019-10-29 | 河南中医药大学 | Tissue specimen fluorescent staining technique time-temperature controls equipment |
-
2007
- 2007-11-07 CN CNU2007200975454U patent/CN201149563Y/en not_active Expired - Fee Related
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105980827A (en) * | 2013-12-13 | 2016-09-28 | 文塔纳医疗系统公司 | Thermal management in the context of automated histological processing of biological specimens and associated technology |
| US10416176B2 (en) | 2013-12-13 | 2019-09-17 | Ventana Medical Systems, Inc. | Staining reagents and other liquids for histological processing of biological specimens and associated technology |
| US10495654B2 (en) | 2013-12-13 | 2019-12-03 | Ventana Medical Systems, Inc. | Thermal management in the context of automated histological processing of biological specimens and associated technology |
| CN105980827B (en) * | 2013-12-13 | 2021-01-05 | 文塔纳医疗系统公司 | Thermal management in the context of automated tissue processing of biological specimens and associated techniques |
| CN104502170A (en) * | 2014-12-24 | 2015-04-08 | 中国人民解放军总医院 | Immobilization device and immobilization method for pathological tissues |
| CN105401367A (en) * | 2015-11-15 | 2016-03-16 | 潘福健 | Water-bath heating type textile dye vat |
| CN108760446A (en) * | 2018-05-18 | 2018-11-06 | 北京林业大学 | A kind of one body swimming bath for arbuscular mycorrhiza trypan blue staining |
| CN110389220A (en) * | 2019-08-28 | 2019-10-29 | 河南中医药大学 | Tissue specimen fluorescent staining technique time-temperature controls equipment |
| CN110389220B (en) * | 2019-08-28 | 2022-06-28 | 河南中医药大学 | Time and temperature control equipment for tissue specimen fluorescent staining technology |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| DD01 | Delivery of document by public notice |
Addressee: Zhao Baozhong Document name: Notification to Pay the Fees |
|
| DD01 | Delivery of document by public notice |
Addressee: Zhao Baozhong Document name: Notification to Pay the Fees |
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| DD01 | Delivery of document by public notice |
Addressee: Zhao Baozhong Document name: Notification to Pay the Fees |
|
| DD01 | Delivery of document by public notice |
Addressee: Zhao Baozhong Document name: Notification of Termination of Patent Right |
|
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20081112 Termination date: 20141107 |
|
| EXPY | Termination of patent right or utility model |