CN201143392Y - Adverse current chromatographic resolution column suitable for separation of small molecules and biomacromolecules - Google Patents
Adverse current chromatographic resolution column suitable for separation of small molecules and biomacromolecules Download PDFInfo
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- CN201143392Y CN201143392Y CNU2007201739463U CN200720173946U CN201143392Y CN 201143392 Y CN201143392 Y CN 201143392Y CN U2007201739463 U CNU2007201739463 U CN U2007201739463U CN 200720173946 U CN200720173946 U CN 200720173946U CN 201143392 Y CN201143392 Y CN 201143392Y
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Abstract
A reflux chromatogram separation column suitable for separating micromolecule and biomacromolecule includes at least a disk; the upper end surface of each disk is provided with at least a spiral circulating slot; the radial direction of the back surface is provided with at least a linear channel; the two sides of each disk are provided with clapboards with pores; the disks and the clapboards are fixed together by an upper flange, a lower flange and a plurality of bolt parts uniformly arranged on the inner circles and the outer circles of the two flanges to form a novel separation column. A hollow autorotation shaft is penetrated in the center of the separation column; a gear is arranged on the upper flange; the reflux chromatogram separation column has the advantages that: 1, the centrifugal force stressed by the liquid in the circulating slot in the flowing process is increased fast by the mode of notching on the disk; a solvent system can be reserved effectively under a higher flow rate of mobile phase; 2, the separation column can be applied to a reflux chromatogram separometer with any mode; 3, the separation column is commonly suitable for separating biomacromolecule and micromolecule matters.
Description
Technical field
The utility model relates to the adverse current chromatogram separation technology field, is a kind of splitter that is used on the high-speed counter-current chromatograph, can generally be applicable to separating of little molecule and large biological molecule material.
Background technology
High speed adverse current chromatogram (High-speed countercurrent chromatography, HSCCC) be the liquid partition chromatography isolation technics of a kind of new type of continuous of growing up in the 1980s, at natural bioactive ingredients, the separation and purification aspect of especially little minute subconstiuent has shown many special advantages in recent years.Because it does not need solid support, the separation of material realizes according to the difference of its distribution coefficient in the immiscible liquid of two-phase, thereby has avoided the problems such as Irreversible Adsorption, inactivation and sex change of separated material.And, make applied sample amount improve greatly because separated material can fully contact between fixing mutually with liquid, be a kind of desirable preparation separation means.
At present, domestic and international business-like high-speed counter-current chromatograph, all adopt multilayer disc around polytetrafluoroethylhelix helix tube column system (as shown in Figure 1).It utilize spiral tube in centrifugal force field synchronous planetary motion and the tangential Archimedian screw power and the radial centrifugal force that produce, make two phase solvent system in helix tube, set up a kind of special one-way fluid dynamic equilibrium, thereby realize the liquid fixedly efficient reservation of phase.Fixing the reservation mutually for the two phase solvent system of separating small-molecule substance can reach more than 70%.At present accumulate a large amount of two-phase solvent systems, comprised two yuan, ternary even quaternary system, can satisfy the separation and purification needs of all kinds of natural small-molecule substances through simple combination and screening.But these dicyandiamide solutions mostly contain organic facies, are not suitable for the separation of protein and other.
For the necessary high viscosity of the big molecule of separating bio, low interfacial tension two phase solvent system, as the double-aqueous phase polymerization objects system, because its viscosity is big, diffusion coefficient is little, and the two-phase density contrast is also very little, and separation time is long, occurs the tendency of emulsification in post.The comprehensive field of force that produced by the synchronous planetary motion of spiral tube this moment deficiency so that the two-phase polymer system is set up good one-way fluid dynamic equilibrium, make to tie up on the HSCCC instrument fixedly by polymeric acceptor commonly used the phase retention is lower than 10%, thereby limited the application of high-speed countercurrent chromatography aspect the separating bio macromolecular substances.
Summary of the invention
The purpose of this utility model is the deficiency at the multi-layer helical tubing string of above-mentioned counter-current chromatograph, a kind of novel countercurrent chromatography separation column is provided, adopt the adverse current chromatogram instrument of this splitter can both realize fixingly efficiently keeping mutually, make it can generally be applicable to separation from little molecule to macromolecular substances to containing organic facies two-phase system and double-aqueous phase system.
For achieving the above object, the utility model is taked following technical scheme:
Adopt a kind of countercurrent chromatography separation column, it includes the center axis of rotation, upper flange, lower flange, between upper and lower flange, be provided with at least one or a plurality of disk, both sides at each disk are provided with dividing plate, the described center axis of rotation is hollow, be applied in the center of upper flange, lower flange, disk and dividing plate, on upper flange, be arranged with planetary gear.Be provided with at least one helical type circulation groove in the upper surface of described each disk, be provided with at least one narrow linear pattern passage at its back side in the radial direction, in order to realize between a plurality of circulation grooves on the same disk and the polyphone between the circulation groove on the different disk.Outer edge at upper flange, lower flange, disk and dividing plate is provided with a plurality of bolt members, and upper and lower flange, disk and dividing plate are fixed together.
Described helical type circulation groove is single head or bull helical type circulation groove.
Described single head helical type circulation groove has head end mouth and tail end mouth, the head end mouth is located at the position near the axis of rotation of disk, the tail end mouth is located at the position near disk border, and be provided with an aperture, this aperture is communicated with an end of one of the disk back side narrow linear pattern passage, the other end of this linear pattern passage is corresponding Yu the head end mouth of the circulation groove of another disk of Xiang Ling, correspondence position on dividing plate between two disks is provided with the dividing plate aperture, make the tail end mouth of the circulation groove of a disk pass through aperture, the linear pattern passage, the dividing plate aperture is connected with the head end mouth of the helical type circulation groove of next disk, realizes the series connection between the helical type circulation groove on the different disks.
Every helical type circulation groove of described bull helical type circulation groove has head end mouth and tail end mouth, and the head end mouth is located at the position near the axis of rotation of disk, and the tail end mouth is located at the position near disk border, and head end mouth and tail end mouth are corresponding on disk evenly to be provided with;
Wherein, article one, do not establish aperture in the head end mouth of helical type circulation groove, be provided with aperture in the tail end mouth, be equipped with aperture with the tail end mouth in the head end mouth of all the other every helical type circulation groove, aperture is communicated with an end of one of the disk back side narrow linear pattern passage in the tail end mouth of every helical type circulation groove, aperture in the head end mouth of the other end of this linear pattern passage and next bar helical type circulation groove of same disk is communicated with, makes to realize between the helical type circulation groove on the same disk connecting; The first port of corresponding article one helical type circulation groove is provided with the dividing plate aperture on dividing plate, the last tail end mouth of the circulation groove of a disk is communicated by the head end mouth of linear pattern passage with the helical type circulation groove of next disk, realize the series connection between the helical type circulation groove on the different disks.
The through hole of a corresponding dividing plate aperture respectively is set on upper flange, lower flange, and head end mouth and the last item helical type circulation groove tail end mouth that will constitute the helical type circulation groove of article one on the disk of splitter are respectively drawn with two polyfluortetraethylene pipes by interface.Then this splitter is installed on the planet carrier of high-speed counter-current chromatograph along axis of rotation direction level or vertical ground, make the hollow shaft of its autobiography axle and planet carrier parallel, article two, the polytetrafluoroethylene (PTFE) pipeline is drawn counter-current chromatograph by the center unwinding of the hollow hollow shaft of the hollow axis of rotation by splitter and planet carrier, and two-port links to each other with detector with the injector of peripheral hardware respectively.
Advantage of the present utility model is:
1, the mode by cutting on disk make liquid suffered centrifugal force in flow process in the circulation groove be fast gradient increases, and the speed that increases increases with the increase of pitch.And traditional multi-layer helical pipe adverse current chromatogram post, the growth of the centrifugal force that each helical layer liquid in pipe is suffered is subjected to the restriction of its caliber (maximum pitch is the external diameter of pipe), increasess slowly (as shown in Figure 1).Just because of the quick growth of this centrifugal force, can under higher flow rate of mobile phase, realize the fixedly efficient reservation of phase, especially the retention rate to double-aqueous phase system is significantly increased.
2, the machining accuracy of this splitter can be controlled by parameters, is easy to Mechanized Processing, and therefore reappearance is better between the disk post that processes, and helps large-scale industrial production.
3, this splitter can be applicable on the adverse current chromatogram separator of any pattern.
4, this splitter generally is applicable to separating of large biological molecule and small-molecule substance.
Description of drawings
Fig. 1 is existing multi-layer helical pipe countercurrent chromatography separation column structural representation.
Fig. 2 is the sectional structure schematic diagram of splitter of the present utility model.
Fig. 3 is Fig. 2 plan structure schematic diagram.
Fig. 4 is a single head helical type circulation groove disc structure schematic diagram of the present utility model.
Fig. 5 is that the sectional structure schematic diagram is looked on the left side of Fig. 4 disk.
Fig. 6 is a diaphragm structure schematic diagram of the present utility model.
Fig. 7 is that the sectional structure schematic diagram is looked on the left side of Fig. 6 dividing plate.
Fig. 8 is a upper flange structural representation of the present utility model.
Fig. 9 is that the sectional structure schematic diagram is looked on the left side of Fig. 8 upper flange.
Figure 10 is a lower flange structural representation of the present utility model.
Figure 11 is that the sectional structure schematic diagram is looked on the left side of Figure 10 lower flange.
Figure 12 is a four-head helical type circulation groove disc structure schematic diagram of the present utility model.
Figure 13 is that Figure 12 four-head helical type circulation groove disk is overlooked the sectional structure schematic diagram.
Figure 14 is that the utility model is used in the structural representation on the high speed adverse current chromatogram centrifugation instrument.
The specific embodiment
Referring to Fig. 2～shown in Figure 14: a kind of countercurrent chromatography separation column, it includes the center axis of rotation 1, upper flange 2, lower flange 5, last, be provided with at least one disk 4 between the lower flange, be provided with dividing plate 3 in the both sides of each disk 4, the described center axis of rotation 1 is hollow, be applied in upper flange, lower flange, the center of disk and dividing plate, be provided with at least one helical type circulation groove 401 in the upper surface of described each disk 4, at upper flange 2, lower flange 5, disk 4 and dividing plate 3 outer edge be provided with a plurality of bolt members 7, with upper flange 2, lower flange 5, disk 4 and dividing plate 3 are fixed together.Be arranged with gear 6 on the upper flange.
The material of disk 4 can adopt stainless steel, plastics or any corrosion resistant macromolecular material.The material of disk 4 adopts high-density polyethylene material in the present embodiment, and the diameter of disk 4 adopts 20cm, and disk 4 thickness are 6mm.Dividing plate 3 can adopt inert material, preferred good seal performance.Present embodiment median septum 3 adopts 1mm thickness polytetrafluoroethylene (PTFE) plate.
See Fig. 4, shown in Figure 5: described single head helical type circulation groove 40 1 has head end mouth 402 and tail end mouth 403, head end mouth 402 is located at the position near the autobiography axle of disk, tail end mouth 403 is located at the position near disk border, be provided with aperture 405 in the tail end mouth 403, this aperture is communicated with an end of one of the disk back side narrow linear pattern passage 404, the other end of this linear pattern passage 404 is corresponding Yu the head end mouth 402 of the circulation groove of another disk of Xiang Ling, the corresponding position is provided with dividing plate aperture 301 and (sees Fig. 6 on the dividing plate between two disks 3, Fig. 7), make the tail end mouth of the circulation groove of a disk pass through aperture, linear pattern passage 404, dividing plate aperture 301 is connected with the head end mouth of the helical type circulation groove of next disk, realizes the series connection between the helical type circulation groove on the different disks.
Single head helical type circulation groove 401 its cross sectional shapes can be rectangle or semicircle, and its size can be provided with as required.The circulation groove that adopts in the present embodiment is wide 2.5mm, the rectangular channel of dark 2.0mm.This single head helical type circulation groove 401 is connected with single head helical type circulation groove on another separator disk.Concrete mode of communicating is: the outer end tail end mouth 403 of this single head helical type circulation groove is an aperture (about 1mm diameter), a thin narrow linear pattern passage 404 at this hole and the disk back side (1 * 1mm) links to each other, this linear pattern passage extend from outside to inside up to the corresponding position of circulation groove head end mouth.This position links to each other with dividing plate aperture 301 on next dividing plate, and this dividing plate aperture 301 (about 1mm diameter) is connected with the head end mouth 402 of circulation groove on the next adjacent discs again.
See shown in Figure 3: the interval by the outward flange that is arranged on disk and inward flange between a plurality of disks 4 and the dividing plate 3 equates that a plurality of bolt members 7 are fixed together, and forms the polydisc splitter.Distance in the present embodiment between the inward flange screw is one of 45 degree, and the distance between the outward flange screw is one of 10 degree.On all disks 4, dividing plate 3, upper flange 2, lower flange 5, same screw 20 is set all.
See Fig. 8, shown in Figure 10: the through hole 201,501 of a corresponding dividing plate aperture 301 respectively is set, respectively the head end mouth 402 of the helical type circulation groove of disk and two polyfluortetraethylene pipes 9 of tail end mouth 403 usefulness is drawn in 1 from hollow center autobiography axle on upper flange 2, lower flange 5.On upper flange 2, be arranged with planetary gear 6.
According to using needs, can arrange single head helical type circulation groove 401 or bull helical type circulation groove 406 on the disk 4.
Referring to Figure 12, shown in Figure 13: be to be furnished with four-head helical type circulation groove disc structure schematic diagram on the disk, on disk 4, all be staggeredly equipped with four helical type circulation grooves 407, be provided with four narrow linear pattern passages 411 in the disk back side radial direction head end mouth 408 and the tail end of four helical type circulation grooves 407 is together in series for 409 mouthfuls.
The head end mouth 408 of every helical type circulation groove 407 is located at the position near the hollow axis of disk, and tail end mouth 409 is located at the position near disk border, and head end mouth 408 and tail end mouth 409 are corresponding on disk evenly to be provided with;
Wherein, article one, do not establish aperture in the head end mouth 408 of helical type circulation groove 407, be provided with aperture 410 in the tail end mouth 409, be provided with aperture 410 with tail end mouth 409 in the head end mouth 408 of all the other every helical type circulation groove 407, aperture 410 is communicated with an end of one of the disk back side narrow linear pattern passage 411 in the tail end mouth 409 of every helical type circulation groove 407, aperture 410 in the head end mouth 408 of the other end of this linear pattern passage 411 and next bar helical type circulation groove 407 of same disk is communicated with, makes to realize between the helical type circulation groove 407 on the disk connecting.Head end mouth 408 places of corresponding article one helical type circulation groove 407 are provided with dividing plate aperture 301 on dividing plate 3, the last tail end mouth of the circulation groove of a disk is communicated by the head end mouth of linear pattern passage with the helical type circulation groove of next disk, realize the series connection between the helical type circulation groove on the different disks.
For the single-screw circulation groove, the bull helical type that is crisscross arranged on disk circulation groove 406 can make the pitch of circulation groove be greatly improved, thereby more favourable to the reservation of dicyandiamide solution.Figure 12, Figure 13 have shown the structure chart of a four-head helical type circulation groove disk.Four helical type circulation grooves 407 are arranged on this disk, and every helical type circulation groove 407 has a head end I
1, I
2, I
3, I
4With a tail end O
1, O
2, O
3, O
4The tail end of every helical type circulation groove 407 just with the head end of next bar helical type circulation groove 407 one in the radial direction, as O
1With I
2, O
2With I
3, O
3With I
4, O
4With I
1At the head end of each bar helix and tail end an aperture 410 (the head end I of article one helical type circulation groove wherein is set all like this
1Do not establish aperture), like this by being engraved in the thin narrow linear pattern passage 411 of disk back side correspondence position, realize on the same separator disk series connection between many helical type circulation grooves 407.The pitch of four screw type circulation grooves 407 that are crisscross arranged like this can improve four times than the pitch of single-screw circulation groove.
As the connection between the single head helical type circulation groove separator disk, the head end of the multi-head spiral type circulation groove that forms through series connection and tail end are connected with bull helical type circulation groove on the adjacent discs by the aperture on the dividing plate respectively.Equally, a plurality of screws and the screw that equate by the interval of the outward flange that is arranged on dish and inward flange between a plurality of disks are fixed together, and form the polydisc splitter.
Referring to shown in Figure 14: it is that the utility model is used in the structural representation on the high-speed counter-current chromatograph;
With a splitter that constitutes by disk 4, dividing plate 3, upper flange 2, the lower flange 5 of five layers of single head helical type circulation groove (the about 100mL of column capacity), be assembled on the planetary centrifugation instrument according to mode shown in Figure 14.This planetary centrifugation instrument comprises: planet carrier 14, casing 21, hollow hollow shaft 28, centre wheel 22, balancing disk 23, planetary gear 6 and 24, driving-belt 25, motor 26, frequency converter 27; Two polytetrafluoroethylene (PTFE) runner pipes 9 that through hole 201 and 501 from last lower flange is drawn, the hollow axis of rotation 1 center by the disk post, finally draw instrument box 21, link to each other with detector with the injector of peripheral hardware respectively then from hollow hollow shaft 28 centers of instrument.
Investigation is equipped with the utility model splitter counter-current chromatograph (Figure 14) to opposed polarity dicyandiamide solution such as chloroform-methanol-water (4: 3: 2), n-butanol-acetate-water (5: 1: 4), 12.5% cetomacrogol 1000-12.5% aqueous phosphatic system, systems such as 4% polyethylene glycol 8000-5% Dextran T 500-91% water, under the different operating condition, as splitter clockwise, be rotated counterclockwise, the upper and lower phase of dicyandiamide solution is made the phase that flows respectively, flow from the head end to the tail end and the fixedly phase retention behavior of tail end under the multiple conditions such as different circulation styles of head end.Found that, for the chloroform-methanol-water of low pole (4: 3: 2, v/v/v) system, at rotating speed is under the 800rpm situation about being rotated counterclockwise, down (during (0.5-7.0mL/min) variation, fixedly the phase retention all can remain on more than 80% in a big way in for I → O), flow velocity for the phase that flows mutually.Under low flow velocity, fixedly the retention of phase can reach more than 90%.(4: 1: 5, v/v/v) system was that 800rpm turns clockwise at rotating speed, is down that (I → O), when flow velocity changed in (0.5-3.0mL/min) scope, fixedly the phase retention can remain on more than 70% mobile phase mutually for n-butanol-acetate-water.For 12.5% cetomacrogol 1000-12.5% aqueous phosphatic system, be that 800rpm turns clockwise at rotating speed, (I → O), when flow velocity changed in (0.5-3.0mL/min) scope, fixedly the phase retention can remain on more than 65% phase in order to flow mutually down.For 4% polyethylene glycol 8000-5% Dextran T 500-91% aqueous systems, under lower mobile phase condition, fixedly the retention of phase can reach nearly 50%.
From above-mentioned experimental result as can be seen, this countercurrent chromatography separation column all is significantly improved for the fixing reserve capability mutually of low pole, strong polarity and double-aqueous phase system.For the low pole system, can realize the fixedly efficient reservation of phase under the high flow rate, this is for the separative efficiency that improves small-molecule substance, and it is very meaningful to shorten disengaging time.Increase significantly for the n-butanol-acetate-aqueous systems of strong polarity and the more traditional spiral tube of reserve capability of double-aqueous phase polymerization objects system, this is for the application of expansion adverse current chromatogram, and a brand-new approach has been opened up in the separation that is used for peptide class, protein and other.
Claims (10)
1, a kind of countercurrent chromatography separation column that is applicable to that little molecule separates with large biological molecule, it is characterized in that: it includes and is provided with a disk (4) at least, the upper surface of each disk (4) is provided with at least one helical type circulation groove (401), this helical type circulation groove (401) has head end mouth (402) and tail end mouth (403), head end mouth (402) is located at the position near the center autobiography axle of disk, and tail end mouth (403) is located at the position near disk border.
2, a kind of countercurrent chromatography separation column that is applicable to that little molecule separates with large biological molecule according to claim 1, it is characterized in that: each disk (4) is provided with at least one linear pattern passage (404) in the radial direction at its back side, in order to being communicated with many helical type circulation grooves on the same disk, and with adjacent discs on helical type circulation groove (401) between be communicated with.
3, a kind of countercurrent chromatography separation column that is applicable to that little molecule separates with large biological molecule according to claim 2, it is characterized in that: the both sides of each disk (4) are provided with dividing plate (3), go up corresponding disk head end mouth (402) at dividing plate (3) and locate to be provided with aperture (301), make and realize between the circulation groove on the adjacent discs being communicated with.
4, a kind of countercurrent chromatography separation column that is applicable to that little molecule separates with large biological molecule according to claim 3, it is characterized in that: it has a hollow center autobiography axle (1), upper flange (2), lower flange (5), on upper flange, be provided with a gear, described center autobiography axle (1) is applied in the center of upper flange, lower flange, disk and dividing plate, evenly is provided with they fixing a plurality of bolt members (7) together in described outer edge at upper flange (2), lower flange (5), disk (4) and dividing plate (3).
5, a kind of countercurrent chromatography separation column that is applicable to that little molecule separates with large biological molecule according to claim 4 is characterized in that: its cross section of helical type circulation groove is a rectangle or semi-circular.
6, a kind of countercurrent chromatography separation column that is applicable to that little molecule separates with large biological molecule according to claim 5 is characterized in that: described helical type circulation groove is a single head helical type circulation groove (401); Described single head helical type circulation groove (401) has head end mouth (402) and tail end mouth (403), head end mouth (402) is located at the position near the center axis of rotation of disk, tail end mouth (403) is located at the position near disk border, be provided with aperture (405) in the tail end mouth (403), this aperture is communicated with an end of the narrow linear pattern passage (404) that the disk back side radially is provided with, and the other end of this linear pattern passage (404) is corresponding Yu the head end mouth of the circulation groove of another disk of Xiang Ling; Corresponding head end mouth (402) position on dividing plate (3) between two disks is provided with dividing plate aperture (301), the tail end mouth (403) of the circulation groove of a disk is connected with the head end mouth of the helical type circulation groove of next disk by aperture, linear pattern passage (404), dividing plate aperture (301) in it, realizes the series connection between the helical type circulation groove on the different disks.
7, a kind of countercurrent chromatography separation column that is applicable to that little molecule separates with large biological molecule according to claim 5 is characterized in that: described helical type circulation groove is a bull helical type circulation groove (406); Every helical type circulation groove (407) of described bull helical type circulation groove (406) has head end mouth (408) and tail end mouth (409), head end mouth (408) is located at the position near the center axis of rotation of disk, tail end mouth (409) is located at the position near disk border, and head end mouth (408) and tail end mouth (409) are corresponding on disk evenly to be provided with;
Wherein, article one, do not establish aperture in the head end mouth (408) of helical type circulation groove (407), be provided with aperture (410) in the tail end mouth (409), be equipped with aperture (410) with tail end mouth (409) in the head end mouth (408) of all the other every helical type circulation groove (407), the interior aperture of tail end mouth (409) (410) of every helical type circulation groove (407) is communicated with an end of a narrow linear pattern passage (411) that radially is provided with at the disk back side, aperture (410) in the head end mouth (408) of the other end of this linear pattern passage (411) and next bar helical type circulation groove (407) of same disk is communicated with, makes to realize between the helical type circulation groove (407) on the same disk connecting; Dividing plate between two disks (3) is gone up the head end mouth (408) of corresponding article one helical type circulation groove (407) and is located to be provided with dividing plate aperture (301), the linear pattern passage of last tail end mouth by the disk back side of the circulation groove of a disk communicated with the head end mouth of the helical type circulation groove of next disk, realize the series connection between the helical type circulation groove on the different disks.
8, according to claim 6 or 7 described a kind of countercurrent chromatography separation columns that are applicable to that little molecule separates with large biological molecule, it is characterized in that: the through hole (201,501) of a corresponding dividing plate aperture (301) respectively is set on upper flange (2), lower flange (5), will constitutes the head end mouth (402) of article one helical type circulation groove of disk of splitter and the tail end mouth (403) of the last item helical type circulation groove respectively and draw with two polyfluortetraethylene pipes (9) by interface.
9, according to claim 6 or 7 described a kind of countercurrent chromatography separation columns that are applicable to that little molecule separates with large biological molecule, it is characterized in that: the center axis of rotation (1) direction level or perpendicular to ground installation on the planet carrier of high-speed counter-current chromatograph, make the hollow hollow shaft (28) of its center axis of rotation (1) and planet carrier parallel.
10, a kind of countercurrent chromatography separation column that is applicable to that little molecule separates with large biological molecule according to claim 8, it is characterized in that: two polyfluortetraethylene pipes (9) are drawn counter-current chromatograph by the center unwinding of the hollow hollow shaft (28) of the center axis of rotation (1) and planet carrier, and two-port links to each other with detector with the injector of peripheral hardware respectively.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2007201739463U CN201143392Y (en) | 2007-11-01 | 2007-11-01 | Adverse current chromatographic resolution column suitable for separation of small molecules and biomacromolecules |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2007201739463U CN201143392Y (en) | 2007-11-01 | 2007-11-01 | Adverse current chromatographic resolution column suitable for separation of small molecules and biomacromolecules |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102282464A (en) * | 2008-11-18 | 2011-12-14 | Cc生物技术有限责任公司 | Countercurrent chromatography rotor |
-
2007
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102282464A (en) * | 2008-11-18 | 2011-12-14 | Cc生物技术有限责任公司 | Countercurrent chromatography rotor |
| US8597509B2 (en) | 2008-11-18 | 2013-12-03 | Cc Biotech Llc | Countercurrent chromatography rotor |
| CN102282464B (en) * | 2008-11-18 | 2015-08-26 | Cc生物技术有限责任公司 | Countercurrent chromatography rotor |
| US9713778B2 (en) | 2008-11-18 | 2017-07-25 | Cc Biotech Llc | Countercurrent chromatography rotor |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term |
Granted publication date: 20081105 |