CN201075106Y - Chip for detecting erythrocyte deformation ability - Google Patents
Chip for detecting erythrocyte deformation ability Download PDFInfo
- Publication number
- CN201075106Y CN201075106Y CNU200720067055XU CN200720067055U CN201075106Y CN 201075106 Y CN201075106 Y CN 201075106Y CN U200720067055X U CNU200720067055X U CN U200720067055XU CN 200720067055 U CN200720067055 U CN 200720067055U CN 201075106 Y CN201075106 Y CN 201075106Y
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- blood cell
- red blood
- membrane
- utility
- model
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- Expired - Fee Related
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- 210000003743 erythrocyte Anatomy 0.000 title claims abstract description 111
- 239000012528 membrane Substances 0.000 claims abstract description 50
- 238000001514 detection method Methods 0.000 claims abstract description 19
- 230000002209 hydrophobic effect Effects 0.000 claims abstract description 7
- 239000011148 porous material Substances 0.000 claims description 5
- 210000004276 hyalin Anatomy 0.000 claims description 2
- 210000004369 blood Anatomy 0.000 abstract description 4
- 239000008280 blood Substances 0.000 abstract description 4
- 238000005259 measurement Methods 0.000 abstract description 4
- 239000010410 layer Substances 0.000 abstract 2
- 210000004492 nuclear pore Anatomy 0.000 abstract 2
- 208000024172 Cardiovascular disease Diseases 0.000 abstract 1
- 238000010521 absorption reaction Methods 0.000 abstract 1
- 238000007877 drug screening Methods 0.000 abstract 1
- 230000002265 prevention Effects 0.000 abstract 1
- 239000002344 surface layer Substances 0.000 abstract 1
- 230000002969 morbid Effects 0.000 description 7
- 239000004743 Polypropylene Substances 0.000 description 5
- HGAZMNJKRQFZKS-UHFFFAOYSA-N chloroethene;ethenyl acetate Chemical compound ClC=C.CC(=O)OC=C HGAZMNJKRQFZKS-UHFFFAOYSA-N 0.000 description 5
- -1 polypropylene Polymers 0.000 description 5
- 229920001155 polypropylene Polymers 0.000 description 5
- 210000000601 blood cell Anatomy 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 239000003146 anticoagulant agent Substances 0.000 description 3
- 229940127219 anticoagulant drug Drugs 0.000 description 3
- 238000010790 dilution Methods 0.000 description 3
- 239000012895 dilution Substances 0.000 description 3
- 230000008595 infiltration Effects 0.000 description 3
- 238000001764 infiltration Methods 0.000 description 3
- 206010008111 Cerebral haemorrhage Diseases 0.000 description 2
- 206010008190 Cerebrovascular accident Diseases 0.000 description 2
- 241000121268 Erythroparvovirus Species 0.000 description 2
- 208000006011 Stroke Diseases 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 230000006806 disease prevention Effects 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 206010018910 Haemolysis Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008588 hemolysis Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 239000012466 permeate Substances 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000011896 sensitive detection Methods 0.000 description 1
Images
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/103—Detecting, measuring or recording devices for testing the shape, pattern, colour, size or movement of the body or parts thereof, for diagnostic purposes
- A61B5/107—Measuring physical dimensions, e.g. size of the entire body or parts thereof
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
- G01N2015/012—Red blood cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N2015/1493—Particle size
- G01N2015/1495—Deformation of particles
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- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model discloses a medical detection device - a red blood cell deformation capacity detection chip, which aims at providing a device to directly measure the red blood cell deformation capacity and providing an important scientific detection means for prevention of cardiovascular diseases, observation of efficacies, drug screening and so on. The principle is that the utility model makes use of micro holes with various hole diameters of red blood cell selective permeable membrane (nuclear pore membrane) to simulate the capillaries with various hole diameters, so the deformation capacity of the red blood cell can be determined by the observation of the capacity of passing through the nuclear pore membrane with various hole diameters of the red blood cell. The structure of the utility model is composed of a surface layer, a group of red blood cell selective permeable membranes with standard hole diameter, an absorption layer and a hydrophobic transparent bottom layer. The red blood cell deformation index can be determined by dropping only one drop of blood on the red blood cell selective permeable membrane and observing whether the blood is permeable. The utility model does not need a power supply and has simple structure, intuitive measurement, convenient usage and low cost.
Description
Technical field
The utility model patent relates to a kind of medical measuring element, particularly a kind of sensitive detection parts of red blood cell deformability.
Technical background
The reduction of human erythrovirus deformability will cause angiocardiopathies such as cerebral hemorrhage, apoplexy, and will be therefore very important to observation, the research of medicine, the screening of red blood cell deformation energy force measurement prevention of disease, curative effect.
The red blood cell of present domestic use distortion detector is by the development of former Shanghai first medical college, and it is to utilize condition of negative pressure under the measurement red blood cell to determine erythrocytic deformability (red blood cell deformation index) by the flow velocity of the nucleopore membranes of 5 micron pore size.
Its weak point is: be not directly to measure erythrocytic deformability, it needs negative pressure equipment, fluid-velocity survey and control device simultaneously, and complex structure, price are more expensive.
Summary of the invention
1, the technical issues that need to address
The problem that the utility model need solve is to measure the red blood cell deformability in simple, intuitive ground.
2, technical scheme
The mean diameter of human erythrovirus is about 7.5 microns, and normal red blood cell is easy to by the capillary of 3 micron diameters haemolysis (the red blood cell wall breaks) not take place.Just become morbid state when the red blood cell deformability reduces can not be by 3 microns the time, further develop, angiocardiopathies such as apoplexy, cerebral hemorrhage may take place.
The micropore of nucleopore membranes is straight-through capillary, and pore size is even, accurate, and reputation is the filter membrane in standard aperture in the world.
Utilize the micropore of the nucleopore membranes in various apertures to simulate the capillary in various apertures, red blood cell is by the ability of various apertures nucleopore membranes, also be equivalent to the ability of red blood cell by the capillary of various diameters, therefore observe the ability of red blood cell, just can measure erythrocytic deformability by the nucleopore membranes in various apertures.
A kind of red blood cell deformability detection chip of novel practical, its architectural feature at least by superficial layer, one group have red blood cell permoselective membrane, the adsorbed layer in standard aperture, the transparent underlayer of hydrophobic nature is formed, superficial layer has some holes, the different red blood cell permoselective membrane of a slice pore size is respectively arranged below each hole, below every red blood cell permoselective membrane, an adsorbed layer is arranged, the bottom is the hyaline membrane that one deck has hydrophobic nature, it and the bonding or heat seal of superficial layer.
When the red blood cell that contains anti-coagulants of dilution drops on the red blood cell permoselective membrane, because the difference of red blood cell deformability, red blood cell can see through some red blood cell permoselective membrane, but can not pass through other red blood cell permoselective membranes, according to the number of passing through, just can measure the red blood cell deformation index:
Red blood cell deformation index=red blood cell can be by the sum of red blood cell permoselective membrane in the number/detection chip of red blood cell permoselective membrane
The difference that is provided with according to red blood cell selective permeation membrane aperture, when comprise in the red blood cell deformability detection chip general red blood cell can not by and during red blood cell permoselective membrane that red little blood cell can pass through:
Red blood cell deformation index=red blood cell can be by number/(sum of red blood cell permoselective membrane-1 in the detection chip) of red blood cell permoselective membrane
When the red blood cell deformation index equals 1, illustrate that the red blood cell deformability is normal, when the red blood cell deformation index less than 1 the time, illustrate that the red blood cell deformability is own undesired, become morbid state.The red blood cell deformation index is littler, and its red blood cell deformability is littler.
A kind of red blood cell deformability detection chip, red blood cell permoselective membrane, adsorbed layer, hydrophobic nature transparent underlayer that its feature has the standard aperture by superficial layer, a slice at least etc. formed, this sheet has the aperture of the red blood cell permoselective membrane in standard aperture can both be passed through for normal person's red blood cell, and the red blood cell of morbid state can not pass through, and what this red blood cell permoselective membrane with standard aperture adopted is the nucleopore membranes of aperture 2.7-3.2 micron.
When the red blood cell drop of anti-freezing has on the red blood cell permselective layer in standard aperture, observe red blood cell and whether see through and just can determine whether red blood cell is ill.
Above the nucleopore membranes that adopts of described red blood cell permoselective membrane, also can claim: the nuclear track etching-film.
3, beneficial effect
The utility model also has except measuring intuitively the red blood cell deformability:
A) simple in structure: as not need complicated instrument and equipment, do not have the equipment of malleation or negative pressure, also do not have the device of fluid-velocity survey and control.This red blood cell deformability detection chip also need not power supply as a card, as long as bleed one with dropping in top can the detection after the anti-coagulants dilution.
B) economy: general red blood cell deformability analyzer needs about 16,000 yuan one, detects one less important about 100 yuan.And using this red blood cell deformability detection chip not need to increase any instrument and equipment, the cost of chip has only one of several yuan.
C) easy to use: as not need complicated operations,, observe red blood cell and whether permeate, can detect as long as one bleed and drop on the red blood cell permoselective membrane with standard aperture after anti-coagulants dilution.Therefore any large and small hospital and remote commune hospital can both use, and particularly China area is wide, populous, differentials between town and country are bigger, and popularizing of prevention of disease, inspection, treatment is more meaningful.
Description of drawings
Figure the one, the 2nd, a kind of red blood cell deformability detection chip of energy measurement red blood cell deformation index.
Figure three is a kind of more economical types, can measure red blood cell morbid state whether red blood cell deformability detection chip.
1 is the superficial layer of red blood cell distortion detection chip among the figure, the 2nd, and red blood cell permoselective membrane, the 3rd, adsorbed layer, the 4th, bottom.
Embodiment
Embodiment one: on the film that polymeric materials such as a tygon or polypropylene are made, the hole that 8 millimeters of 6 diameters are arranged, as superficial layer, be that diameter is that 12 millimeters the nucleopore membranes that has various kapillaries aperture respectively is as the red blood cell permselective layer below each hole, their aperture is respectively 2,3,3.5,4,5,8 microns, the general red blood cell of the nucleopore membranes of 2 micron pore size not porous passes through, and only suffers from red little blood cell increase disease, and red little blood cell could pass through.The normal red blood cell of the nucleopore membranes in other aperture can both pass through, when red blood cell can not be by some aperture wherein, the red blood cell wan, be that diameter is that 16 millimeters filter paper is as adsorbed layer below nucleopore membranes, bottom one deck also be tygon or polypropylene as bottom, compress bonding between them.When being diluted to 20% droplet of blood on every nucleopore membranes the time, can observe erythrocytic infiltration situation at reverse side, just can determine the red blood cell deformation index:
Red blood cell deformation index=red blood cell can be by number/(sum of red blood cell permoselective membrane-1 in the detection chip) of red blood cell permoselective membrane
When the red blood cell deformation index greater than 1 the time, be red little blood cell increase disease.When the red blood cell deformation index equals 1, for normally.When the red blood cell deformation index less than 1 the time, red blood cell is a morbid state.The red blood cell deformation index is littler, illustrates that erythrocytic deformability is poorer.
Embodiment two: on the film that polymeric materials such as a tygon or polypropylene are made, the hole that 8 millimeters of 5 diameters are wherein arranged, as superficial layer, be that diameter is that 12 millimeters the nucleopore membranes that has various kapillaries aperture respectively is as the red blood cell permselective layer below each hole, their aperture is respectively 2.8,3.4,4,5,8 microns, the normal red blood cell of the nucleopore membranes in these apertures can both pass through, when red blood cell can not be by wherein some aperture the time, the red blood cell wan, be that diameter is that 16 millimeters face tissue is as adsorbed layer below nucleopore membranes, bottom one deck is that materials such as tygon or polypropylene are made bottom, compresses heat seal between them.When being diluted to 10% droplet of blood on every nucleopore membranes the time, can observe erythrocytic infiltration situation at reverse side, just can determine the red blood cell deformation index:
Red blood cell deformation index=red blood cell can be by the sum of red blood cell permoselective membrane in the number/detection chip of red blood cell permoselective membrane
When the red blood cell deformation index equals 1, for normally.When the red blood cell deformation index less than 1 the time, red blood cell is a morbid state.The red blood cell deformation index is littler, illustrates that erythrocytic deformability is poorer.
Embodiment three: the hole of opening a diameter and be 10 millimeters on a piece of paper sheet, as superficial layer, be that a diameter is that 14 millimeters aperture is that 3 microns nucleopore membranes is as the red blood cell permselective layer below the hole, be as adsorbed layer below it with face tissue, bottom one deck be materials such as tygon or polypropylene make as bottom, compress bonding between them.When being diluted to 30% red blood cell drop on this nucleopore membranes the time, can observe erythrocytic infiltration situation at reverse side, just can determine whether morbid state of red blood cell.
Claims (2)
1. red blood cell deformability detection chip, its architectural feature at least by superficial layer, one group have red blood cell permoselective membrane, the adsorbed layer in standard aperture, the transparent underlayer of hydrophobic nature is formed, superficial layer has some holes, the different red blood cell permoselective membrane of a slice pore size is respectively arranged below each hole, below every red blood cell permoselective membrane, an adsorbed layer is arranged, the bottom is the hyaline membrane that one deck has hydrophobic nature, it and the bonding or heat seal of superficial layer.
2. red blood cell deformability detection chip, its feature are made up of red blood cell permoselective membrane, adsorbed layer, hydrophobic nature transparent underlayer that superficial layer, a slice have a standard aperture at least.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU200720067055XU CN201075106Y (en) | 2007-02-06 | 2007-02-06 | Chip for detecting erythrocyte deformation ability |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU200720067055XU CN201075106Y (en) | 2007-02-06 | 2007-02-06 | Chip for detecting erythrocyte deformation ability |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201075106Y true CN201075106Y (en) | 2008-06-18 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNU200720067055XU Expired - Fee Related CN201075106Y (en) | 2007-02-06 | 2007-02-06 | Chip for detecting erythrocyte deformation ability |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201075106Y (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108445200A (en) * | 2018-02-12 | 2018-08-24 | 南方医科大学 | A kind of influence based on micro-fluidic chip detection pentoxifylline to Erythrocytes from Coronary Heart Disease deformability and biochemical index |
-
2007
- 2007-02-06 CN CNU200720067055XU patent/CN201075106Y/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108445200A (en) * | 2018-02-12 | 2018-08-24 | 南方医科大学 | A kind of influence based on micro-fluidic chip detection pentoxifylline to Erythrocytes from Coronary Heart Disease deformability and biochemical index |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20080618 |