CN1981819B - Tree-peony root bark component, its preparation, making method and use - Google Patents
Tree-peony root bark component, its preparation, making method and use Download PDFInfo
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- CN1981819B CN1981819B CN2005101223493A CN200510122349A CN1981819B CN 1981819 B CN1981819 B CN 1981819B CN 2005101223493 A CN2005101223493 A CN 2005101223493A CN 200510122349 A CN200510122349 A CN 200510122349A CN 1981819 B CN1981819 B CN 1981819B
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Abstract
An active component of tree peony bark is proportionally composed of paeoniflorin and gallic acid. Its preparing process includes such steps as breaking red sage root, adding ethyl acetate and alcohol, thermal extracting, concentrating to obtain extract, mixing it with silicon gel, separating with positive-phase silicon gel column, washing with petroether and ethyl acetate, eluting with chloroform and methanol, concentrating the eluting liquid, drying, and separating by liquid-phase chromatography. Its application is also disclosed.
Description
Technical field
The present invention relates to a kind of Chinese medicine extract for the treatment of cardiovascular disease, relate in particular to the active component that from Cortex Moutan, extracts, preparation and preparation method thereof and purposes, this active component mainly contains peoniflorin, two kinds of chemical compounds of gallic acid.
Background technology
Coronary heart disease and angina pectoris are harm humans health " first killers ", in recent years, along with the variation of China's population senescence and people's work, life, dietary structure and environment etc., the incidence rate of cardiovascular and cerebrovascular diseases such as coronary heart disease also increases year by year, and the people's physical and mental health in serious threat.In the natural product many active substances have resist myocardial ischemia, anoxia functions, some of them have been developed to treatment coronary heart disease and anginal new drug, as treat the medicine DIAOXINXUE KANG of coronary heart disease, it is by 8 kinds of pure Chinese medicinal preparations that steroid saponin is made that extract from Chinese medicine; Flavonihippophae is to be that raw material extracts the pure natural medical that processes with the Fructus Hippophae, and its effective ingredient is a Fructus Hippophae total flavones.Thereby from natural product, seek have resist myocardial ischemia, the active substance of anoxia physiologically active, be find, one of effective way of developing new drug.China's medicinal organism resource is very abundant, and its biological active substances is research and finds new drug guide chemicals, the natural treasure-house of developing new drug.At present, China extracts active substance from natural product, be used to be developed to treatment coronary heart disease, safety is good, toxicity is low new drug also seldom, from natural product, extract active substance, be developed to have and resist myocardial ischemia, be used for the treatment of coronary heart disease and anginal new drug, have significant application value and wide development prospect.
Cortex Moutan has another name called Cortex Moutan, is the root bark of dicotyledon medicine ranunculaceae peony (Paeonia suffruticosa Andr.).Ground such as distribution Hebei, Henan, Shandong, Sichuan, Shaanxi, Gansu.All there is cultivation all parts of the country, ground such as medical material main product Anhui, Sichuan, Gansu, Shaanxi, Hubei, Hunan, Shandong, Guizhou, and in addition, also produce in Yunnan, Zhejiang.Its cold nature, the acrid in the mouth hardship, the heart, liver, kidney channel are gone in cold, have heat clearing away, removing heat from blood, and blood, the effect of repercussive.Cortex Moutan is concocted and to be seen China's beam Dai Taohong scape the earliest and write in " variorums ", thinks: " all urge to break, remove the heart." in the medical book such as Compendium of Material Medica record " the Cortex Moutan core is not used as medicine, and need go it." studies show that: Cortex Moutan contains paeonol (Paeono1), paeoniflorin (Paeoniforin), hydroxypaeoniflorin, benzoylpaeoniflorin (Benzoylpaeoniflorin), benzoyl hydroxypaeoniflorin, Radix Paeoniae aglycon (Paeoniflorigenone), 6-Hydroxycoumarin (6-hydroxycoumarin) and gallic acid (GallicAcid), and benzoic acid, plant sterol, sucrose, glucose, arabinose etc.Contain benzoic acid (Benzoic Acid), tetradecane hydrocarbon (Tetradecane), 2-hydroxyl-4-methoxyl group-1-Phenylethanone. (2-hydroxy-4-methoxy acetophenone), 2 in the volatile oil, the two tertiary butyl 1,4-benzoquinone (2 of 6-, 6-ditert-butyl-p-Benzoquinone), diethyl phthalate (Diethyl Phthalate), isopropyl myristate chemical compound (Chen Yuejiao etc. such as (Isopropyl Myristate), Guangzhou food industry science and technology, 2002,18 (4): 36-37).People such as Wu Shaohua separate from Cortex Moutan first and have obtained following five kinds of chemical compounds: betulic acid (Betulinic Acid), betulin (Betulin), oleanolic acid (OleanolicAcid), 3 β, 23-dihydroxy-30-norolean-12,20 (29)-dien-28-oic acid, 3-O-methylpaeonisuffral (Wu Shaohua etc., Chinese herbal medicine, 2002,33 (8): 679-680).
Cortex Moutan has the degree of injury of alleviating effect to expeirmental myocardial ischemia, and can reduce myocardial oxygen consumption, increases coronary flow; think that Cortex Moutan has that to regulate blood capable, the effect of mediation blood vessels, thereby myocardial ischemia had protective effect (Ma Yuling etc.; the Shanxi medical magazine, 1984,13 (4): 212-214).Peoniflorin has antiplatelet aggregative activity, and antithrombotic effect is to the protective effect and the antitumor action of liver.Peoniflorin has blocking effect (Zhang Guangxin etc., Chinese Pharmacological circular, 2003,19 (8): 863-866) to myocardial cell Ica, L.Peoniflorin can increase neurocyte survival quantity, reduces mortality rate, excitatory neuron damage (Wu Yumei etc., Chinese J Pharmacol Toxicol, 2002,16 (3): 172-175) due to the antagonism KA.Peoniflorin has significant protective effect (Yang Jun etc., Chinese Journal of New Drugs, 2001,10 (6): 426-428) to PC12 cell injury due to the calcium overload.The colony that peoniflorin strengthens syndrome of deficiency of blood mouse bone marrow cells hematopoietic stem/progenitor cells forms ability; Peoniflorin can promote marrow stromal cell secretion Hemopoietic factor (G-CSF, GM-CSF, PDGF-α) etc., suppresses secretion (Gao Yue, Tianjin Chinese medicine, 2003,20 (6): 47-51) of hemopoietic inhibitive factor (M-CIP).
Summary of the invention
The object of the present invention is to provide a kind of moutan bark effective constituent.
Another object of the present invention is to provide the preparation method and its usage of this moutan bark effective constituent.
The present invention also provides the preparation that comprises moutan bark effective constituent.
The present invention is implemented with following technical scheme:
In the moutan bark effective constituent of the present invention, mainly contain peoniflorin and gallic acid.Wherein, by weight percentage, content of paeoniflorin is 70～80%, and the content of gallic acid is 10～20%.
Preferably, in the moutan bark effective constituent of the present invention, by weight percentage, content of paeoniflorin is 72～78%, and the content of gallic acid is 12～18%.
Best, in the moutan bark effective constituent of the present invention, by weight percentage, content of paeoniflorin is 74～76%, the content of gallic acid is 14～16%.
The preparation method of moutan bark effective constituent of the present invention, comprise the following steps: and will add ethyl acetate and ethanol after the Cortex Moutan pulverizing medicinal materials, heating extraction gets extracting solution, and extracting solution is condensed into extractum, and itself and silica gel mixed sample, with normal phase silicagel column it is separated, at first use petroleum ether and ethyl acetate, get eluent I as mobile phase, abandon it, change chloroform and methanol then as mobile phase, get eluent II, will get sample behind the eluent II concentrate drying; Continue to separate the sample that obtains with preparative liquid chromatography; The separation condition of preparative hplc: chromatographic column is a semi-preparative column, and mobile phase is water and acetonitrile, gradient elution.
Preferably, moutan bark effective constituent preparation method of the present invention, comprise the following steps: and to add ethyl acetate and ethanol (1: 0.8～1.2) after the Cortex Moutan pulverizing medicinal materials, reflux 0.8～1.2 hour, extract 1～3 time, merging filtrate gets extracting solution, extracting solution is condensed into extractum, and itself and silica gel are mixed sample, it is separated with normal phase silicagel column, at first use petroleum ether and ethyl acetate (47～53: 1) as mobile phase, get eluent I, abandon it, change chloroform and methanol (8～13: then 1) as mobile phase, get eluent II, will get sample behind the eluent II concentrate drying; Continue to separate the sample that obtains with preparative liquid chromatography; The separation condition of preparative hplc: chromatographic column is a semi-preparative column, and mobile phase is water and acetonitrile, and gradient elution, flow velocity are 2.8～3.2ml/min, and column temperature is a room temperature.
Best, moutan bark effective constituent preparation method of the present invention comprises the following steps: and will add ethyl acetate and ethanol (1: 1) after the Cortex Moutan pulverizing medicinal materials that reflux 1 hour is extracted 2 times, merging filtrate gets extracting solution; Extracting solution is condensed into extractum, and itself and silica gel mixed sample, with normal phase silicagel column it is separated, at first use petroleum ether and ethyl acetate (50: 1) as mobile phase, get eluent I, abandon it, change chloroform and methanol (10: 1) then as mobile phase, get eluent II, will get sample behind the eluent II concentrate drying; Continue to separate the sample that obtains with preparative liquid chromatography; The separation condition of preparative hplc: chromatographic column is semi-preparative column (Lichrospher C18; 10.0mm * 250mm, 5 μ m), mobile phase is water (A) and acetonitrile (B), and the gradient elution program is as follows: in the time of 0 minute, mobile phase A is that 80% aqueous solution, Mobile phase B are 20% acetonitrile solution; In the time of 40 minutes, mobile phase A is that 20% aqueous solution, Mobile phase B are 80% acetonitrile solution; In the time of 50 minutes, mobile phase A is that 5% aqueous solution, Mobile phase B are 95% acetonitrile solution; Flow velocity is 3ml/min, and column temperature is a room temperature; Sample 100% dissolve with ethanol separates through preparative liquid chromatography, collects solution at time period 7.6～9.7min, and solution obtains active component behind concentrate drying.
Moutan bark effective constituent of the present invention can be used as active component, adds the adjuvant of accepting on the pharmaceutics, makes preparation according to the preparation method of the preparation of putting down in writing on the pharmaceutics.
Moutan bark effective constituent of the present invention can also add the adjuvant of accepting on the pharmaceutics as one of active component, makes preparation according to the preparation method of the preparation of putting down in writing on the pharmaceutics.
Described preparation comprises injection, drip liquid, injectable powder, granule, tablet, electuary, powder, oral liquid, sugar coated tablet, film coated tablet, enteric coated tablet, capsule, hard capsule, soft capsule, sucks agent, granule, pill, unguentum, sublimed preparation, spray, drop pill, disintegrating agent, oral cavity disintegration tablet, micropill etc.
Moutan bark effective constituent of the present invention and preparation thereof can be used in preparation treatment, angiocardiopathy preventing medicine.
Beneficial effect of the present invention is:
1. use normal phase silicagel column in the extraction and separation process of the present invention, can remove impurity such as desaccharide, protein, aminoacid effectively, improved content of effective, adopted preparative hplc simultaneously, can obtain effective ingredient fast and accurately.
2. moutan bark effective constituent chemical constituent provided by the invention is simply clear and definite, is easier to illustrate its mechanism of action on pharmacological research, is easier to the quality control of medicine aborning.
Description of drawings
Fig. 1 is the HPLC analysis chart of moutan bark effective constituent of the present invention.
The specific embodiment
Further describe flesh and blood of the present invention and beneficial effect below in conjunction with embodiment, this embodiment only is used to the present invention is described but not limitation of the present invention.
The preparation of embodiment one moutan bark effective constituent
Ethyl acetate and ethanol will be added after the 200g Cortex Moutan pulverizing medicinal materials, heating extraction gets extracting solution, extracting solution is condensed into extractum 16.8g, and itself and silica gel are mixed sample, it is separated with normal phase silicagel column, at first use petroleum ether and ethyl acetate as mobile phase, get eluent I, abandon it, change chloroform and methanol then as mobile phase, get eluent II, will get sample 3.9g behind the eluent II concentrate drying; Continue to separate the sample that obtains with preparative liquid chromatography; The separation condition of preparative hplc: chromatographic column is a semi-preparative column, and mobile phase is water and acetonitrile, gradient elution.Sample 100% dissolve with ethanol separates through preparative liquid chromatography, collects solution at time period 7.6～9.7min, obtains active component 0.34g behind the concentrate drying.
The preparation of embodiment two moutan bark effective constituents
Ethyl acetate and ethanol (1: 0.8～1.2) will be added after the 500g Cortex Moutan pulverizing medicinal materials, reflux 0.8～1.2 hour, extract 1～3 time, merging filtrate gets extracting solution, extracting solution is condensed into extractum 42g, and itself and silica gel mixed sample, with normal phase silicagel column it is separated, at first use petroleum ether and ethyl acetate (47～53: 1) as mobile phase, get eluent I, abandon it, change chloroform and methanol (8～13: then 1) as mobile phase, get eluent II, will get sample 11.8g behind the eluent II concentrate drying; Continue to separate the sample that obtains with preparative liquid chromatography; The separation condition of preparative hplc: chromatographic column is a semi-preparative column, and mobile phase is water and acetonitrile, and gradient elution, flow velocity are 2.8～3.2ml/min, and column temperature is a room temperature.Sample 100% dissolve with ethanol separates through preparative liquid chromatography, collects solution at time period 7.6～9.7min, obtains active component 0.91g behind the concentrate drying.
The preparation of embodiment three moutan bark effective constituents
Get Cortex Moutan medical material 250g, it is pulverized the back add ethyl acetate and ethanol (1: 1), reflux 1 hour is extracted 2 times, filtrate merge extracting solution.Extracting solution is condensed into extractum gets 22g, with extractum and silica gel mixed sample, separate with normal phase silicagel column, at first use petroleum ether and ethyl acetate (50: 1) as mobile phase, get eluent I, change chloroform and methanol (10: 1) then as mobile phase, get eluent II, get the 6.2g sample behind the concentrate drying.Continue to separate the sample that obtains with preparative liquid chromatography.The separation condition of preparative hplc: chromatographic column is semi-preparative column (the Lichrospher C18 of Chinese nation; 10.0mm * 250mm, 5 μ m), mobile phase is water (A) and acetonitrile (B), and gradient sees Table 2, and flow velocity is 3ml/min, and column temperature is a room temperature.Sample 100% dissolve with ethanol separates through preparative liquid chromatography, collects solution at time period 7.6～9.7min, obtains active component 0.49g behind the concentrate drying.
The analysis of embodiment four moutan bark effective constituents
(1) assay of peoniflorin, gallic acid (high performance liquid chromatography) in the Cortex Moutan extract
Chromatographic conditionChromatographic column Agilent Zorbax SB-C18 post (4.6mm * 150mm, 5 μ m); Adopt gradient elution, mobile phase A is 0.2% glacial acetic acid aqueous solution mutually, and Mobile phase B is mutually for containing the acetonitrile solution of 0.2% glacial acetic acid; The gradient elution program is as follows: in the time of 0 minute, mobile phase A is that 90% 0.2% glacial acetic acid aqueous solution, Mobile phase B are the acetonitrile solution of 10% 0.2% glacial acetic acid; In the time of 10 minutes, mobile phase A is that 50% 0.2% glacial acetic acid aqueous solution, Mobile phase B are the acetonitrile solution of 50% 0.2% glacial acetic acid; In the time of 30 minutes, mobile phase A is that 5% 0.2% glacial acetic acid aqueous solution, Mobile phase B are the acetonitrile solution of 95% 0.2% glacial acetic acid; Flow velocity 0.5mLmin
-1It is long to detect the wavelength all-wave; 30 ℃ of column temperatures; ELSD condition: 100 ℃ of drift tube temperatures; Nitrogen flow rate 1.4L/min
The preparation of need testing solutionTake by weighing this product, in volumetric flask, be diluted to scale, shake up, promptly with dissolve with methanol solution.
Assay methodThe accurate need testing solution of drawing injects chromatograph of liquid, measures, promptly.
(2) above-mentioned Cortex Moutan extract HPLC-ELSD finger printing
Assay methodMeasure (high performance liquid chromatography) referring to the peoniflorin in (1) above-mentioned Cortex Moutan extract, gallic acid content.The record chromatograph time is 30 minutes.
Analysis resultThe HPLC-ELSD analysis of spectra of moutan bark effective constituent is seen Fig. 1, and 1 among Fig. 1,2 is respectively peoniflorin, gallic acid.
Among the present invention, the structural formula of peoniflorin is:
Among the present invention, the structural formula of galloylpaeoniflorin is:
The preparation of embodiment five drop pill
Get moutan bark effective constituent 0.5g and 10.5g Polyethylene Glycol-6000 mix homogeneously of embodiment three, heating and melting moves in the drop pill drip irrigation behind the change material, and in ℃ liquid paraffin of medicine liquid droplet to 6～8, oil removing makes 400 of drop pill.
The preparation of embodiment six lyophilized injectable powders
Get moutan bark effective constituent 0.5g, glucose 4.5g, sodium thiosulfate 0.9g and the distilled water 1ml of embodiment three, behind the said components mix homogeneously, lyophilization, 500 of packing, promptly.
The preparation of embodiment seven lyophilized injectable powders
Get Lignum Dalbergiae Odoriferae oil 1.5g, join in the saturated hydroxypropyl of 13ml, stirring and dissolving filters, and the filtrate cold drying gets the clathrate powder of Lignum Dalbergiae Odoriferae oil and hydroxypropyl.Except that above-mentioned Lignum Dalbergiae Odoriferae oil closes the clathrate powder of hydroxypropyl, get Cortex Moutan extract 0.5g, mannitol 5.5g, calcium disodium edetate 0.9g and the distilled water 2ml of embodiment three again, behind the said components mixing, lyophilization, 300 of packing, promptly.
The pharmacological evaluation of embodiment eight moutan bark effective constituents
1 pharmacological model: neonatal rat myocardial cell hypoxic-ischemic model
Former generation myocardial cell cultivation After 1～3 day SD neonatal rat that is born was put to death, it was dirty to core, and subtracts into 1mm after PBS liquid cleans
3About fragment.With 0.125% trypsin Sigma, USA)+0.05% (Gibco is USA) in 37 degree digestion 3～4 times for collagenase II.The differential adherent method was separated into fibrocyte after 1.5 hours, and cell suspension is transferred to (every hole about 4 * 10 in 48 orifice plates
5Cell).(Gibco, (Falcon, USA) cell of cultivating 3-6 days supplies medicine efficacy screening USA) to add 10% hyclone through DMEM.Screen and replaced with serum-free medium in preceding 1 day.Cultivate 3～6 days myocardial cell, PBS washing back adds the pastille culture fluid.Place 95%N
2And 5%CO
2Anoxia is 6 hours in the incubator.Then at CO
2Reoxygenation is 3 hours in the incubator.Get cell conditioned medium liquid and measure LDH content.
Dosage regimenThe moutan bark effective constituent that extraction obtains is made into 10 with sugar-free Hanks liquid
-4G/mL test liquid, liposoluble constituent can add a small amount of DMSO hydrotropy (the DMSO final concentration is controlled at below 0.2%).The final concentration of each component in culture fluid is controlled at 10
-5G/mL.
The lactic acid dehydrogenase assayLactic acid dehydrogenase content can be represented the cell injury degree in the cell culture fluid.The lactic acid dehydrogenase content that bleeds in the supernatant is high more, shows that cell injury is also remarkable.The determination of lactate dehydrogenase test kit builds up bio-engineering research institute available from Nanjing.Assay method is: get 30 microlitre cell conditioned medium liquid, add 50 microlitre substrate buffer and 10 microlitre lactic dehydrogenase enzyme cofactors, 37 degree vibrations 15 minutes add 50 microlitre 2,4 dinitrophenyl hydrazines again, 37 degree vibrations 15 minutes.Parallel blank.Extract reaction solution 50 microlitres and add 200 microlitre 0.4mol/L sodium hydroxide solutions, leave standstill 3～4 minutes after, measure shading value with microplate reader in 450nm.
Drug effect result
All data are represented with the mean value variance.Adopt monolateral variance analysis and T check carrying out statistical analysis.Compare with model group, P＜0.05 is thought significantly effectively.Drug effect the results are shown in Table 1.According to myocardial cell lactic acid dehydrogenase (LDH) testing result, moutan bark effective constituent has the highly significant effect to reducing LDH release.
Table 1
2 pharmacological models: huve cell anoxia reoxygenation model
Former generation huve cell cultivationGet the healthy newborn umbilical cord, clean the interior residual bloodstain of vein blood vessel with 30～50mlHanks liquid.Look the 0.1% collagenase I that umbilical cord length adds respective amount, change incubator digestion 15～20 minutes over to.Subsequently Digestive system in the umbilical cord is carefully collected in the beaker, and with Hanks liquid with beaker rinse twice, collect in the lump and be sub-packed in centrifuge tube in the beaker, centrifugal 10 minutes of 900rpm.Supernatant is removed in suction, with the abundant dissolution precipitation of M199 culture fluid (contain 10% hyclone, 100U/ml is two anti-, 0.15mg/ml Heparin, 10uM VC).Gained cell suspension is sub-packed in 5cm
2Culture bottle places in the incubator and cultivates.Changed in second day and adopt the M199 culture fluid that contains 30ug/ml ECGS, changed a culture fluid and be paved with the bottom surface in per afterwards three days until cell.Used cell is former generation endotheliocyte.The culture bottle inner cell is seeded to 96 orifice plates before the modeling and cultivated one day, used cell concentration is 3.5～40,000/hole.During modeling, inhale and abandon old M199 culture fluid, adding pastille does not have phenol red M199 culture fluid, and every hole total amount is 100ul.Place 95%N
2And 5%CO
2Anoxia is 12 hours in the incubator, then at CO
2Reoxygenation is 2 hours in the incubator.Get cell conditioned medium liquid and measure LDH content and NO content.
Dosage regimenThe moutan bark effective constituent that extraction obtains is made into 10 with sugar-free Hanks liquid
-4G/mL test liquid, liposoluble constituent can add a small amount of DMSO hydrotropy (the DMSO final concentration is controlled at below 0.2%).The final concentration of each component in no phenol red M199 culture fluid is controlled at 10
-5G/mL.
The lactic acid dehydrogenase assayLactic acid dehydrogenase content can be represented the cell injury degree in the cell culture fluid.The lactic acid dehydrogenase content that bleeds in the supernatant is high more, shows that cell injury is also remarkable.The determination of lactate dehydrogenase test kit builds up bio-engineering research institute available from Nanjing.Assay method is: get 30 microlitre cell conditioned medium liquid, add 50 microlitre substrate buffer and 10 microlitre lactic dehydrogenase enzyme cofactors, 37 degree vibrations 15 minutes add 50 microlitre 2,4 dinitrophenyl hydrazines again, 37 degree vibrations 15 minutes.Parallel blank.Extract reaction solution 50 microlitres and add 200 microlitre 0.4mol/L sodium hydroxide solutions, leave standstill 3～4 minutes after, measure shading value with microplate reader in 450nm.
Drug effect resultAll data are represented with the mean value variance.Adopt monolateral variance analysis and T check carrying out statistical analysis.Compare with model group, P＜0.05 is thought significantly effectively.Drug effect the results are shown in Table 2.According to endotheliocyte lactic acid dehydrogenase testing result, moutan bark effective constituent has the highly significant effect to reducing LDH release.
Table 2
Claims (3)
1. a Cortex Moutan extract is characterized in that, adopts following method preparation:
To add 1: 1 ethyl acetate and ethanol after the Cortex Moutan pulverizing medicinal materials, reflux 1 hour is extracted 2 times, and merging filtrate gets extracting solution; Extracting solution is condensed into extractum, and itself and silica gel mixed sample, with normal phase silicagel column it is separated, at first use 50: 1 petroleum ether and ethyl acetate as mobile phase, eluent I, abandon it, change 10: 1 chloroforms and methanol then as mobile phase, get eluent II, will get sample behind the eluent II concentrate drying; Continue to separate the sample that obtains with preparative liquid chromatography; The separation condition of preparative hplc: chromatographic column is semi-preparative column Lichrospher C18; 10.0mm * 250mm, 5 μ m, mobile phase A is that water and B are acetonitrile, the gradient elution program is as follows: in the time of 0 minute, mobile phase A is that 80% aqueous solution, Mobile phase B are 20% acetonitrile solution; In the time of 40 minutes, mobile phase A is that 20% aqueous solution, Mobile phase B are 80% acetonitrile solution; In the time of 50 minutes, mobile phase A is that 5% aqueous solution, Mobile phase B are 95% acetonitrile solution; Flow velocity is 3ml/min, and column temperature is a room temperature; Sample 100% dissolve with ethanol separates through preparative liquid chromatography, collects solution at time period 7.6～9.7min, and solution obtains active component behind concentrate drying.
2. contain the pharmaceutical formulation of the described Cortex Moutan extract of claim 1, it is characterized in that, make arbitrary pharmaceutical formulation that pharmaceutics is accepted according to the preparation method that pharmaceutics allows.
3. the application of the described Cortex Moutan extract of claim 1 in preparation treatment and angiocardiopathy preventing medicine.
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| CN1273961A (en) * | 2000-01-28 | 2000-11-22 | 中国科学院广州化学研究所 | Process for extracting peonol with supercritical or liquid-state CO2 |
| CN1187069C (en) * | 2002-11-26 | 2005-02-02 | 安徽省天尊医药科技研究所 | Method for extracting coxtex mouten total glucoside for treating hepatitis |
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| CN1273961A (en) * | 2000-01-28 | 2000-11-22 | 中国科学院广州化学研究所 | Process for extracting peonol with supercritical or liquid-state CO2 |
| CN1187069C (en) * | 2002-11-26 | 2005-02-02 | 安徽省天尊医药科技研究所 | Method for extracting coxtex mouten total glucoside for treating hepatitis |
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| 吴少华等.丹皮的化学成分研究.《中草药》.2002,第33卷(第8期),679-680. * |
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