CN1974586A - Aminoglycoside antibiotics enriching and purifying macroporous resin process - Google Patents
Aminoglycoside antibiotics enriching and purifying macroporous resin process Download PDFInfo
- Publication number
- CN1974586A CN1974586A CN 200610135344 CN200610135344A CN1974586A CN 1974586 A CN1974586 A CN 1974586A CN 200610135344 CN200610135344 CN 200610135344 CN 200610135344 A CN200610135344 A CN 200610135344A CN 1974586 A CN1974586 A CN 1974586A
- Authority
- CN
- China
- Prior art keywords
- aminoglycoside antibiotics
- resin
- enriching
- solution
- amount
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
The present invention provides macroporous resin enriching and purifying process for aminoglycoside antibiotics. The process includes the following steps: saturation adsorbing aminoglycoside antibiotics solution with macroporous resin in pH 8.0-9.5, washing off the un-adsorbed residual liquid and impurity in lower adsorption force with water over 1.5 times, eluting aminoglycoside antibiotics component with 30-65 % concentration water solution of hydrophilic solvent in 4-8 times the resin volume, vacuum concentrating the eluted liquid at 0.06-0.095 MPa and 50-65 deg.c to recover solvent, and spray drying to obtain aminoglycoside antibiotics product. The present invention has novel and simple technological process, high product quality and low production cost, and may be used in the separation and purification of aminoglycoside antibiotics solution in different sources.
Description
Technical field
The present invention relates to a kind of separation of antibiotics purification process, more specifically relate to a kind of method that contains the aminoglycoside antibiotics of 2-deoxystreptamine in the enriching and purifying macroporous resin molecular structure that adopts.
Background technology
Aminoglycoside antibiotics is to contain aminosugar in the class formation with glycosidic link form and aminosugar hexalin bonded compound, is the important microbiotic of finding after penicillin early of a class.The sum of the natural and semi-synthetic aminoglycoside antibiotics of having reported at present is above 3000 kinds, wherein the natural aminoglycoside antibiotics of microorganisms has nearly 200 kinds, have clinical use value 30 surplus kind, as Streptomycin sulphate, Xin Meisu, kantlex, gentamicin, micronomicin, ribostamycin, tobramycin and sisomicin etc., also have some semisynthetic microbiotic such as amikacin, amikacin, netilmicin and Etimicin etc. to come out one after another, and be used to clinical.
Molecular structure according to aminoglycoside antibiotics can be divided into streptamine derivative (as Streptomycin sulphate), the 2-deoxystreptamine derivative (comprises 4, the kantlex of 6-disubstituted derivative, tobramycin, gentamicin, micronomicin and sisomicin; 4, the Xin Meisu of 5-disubstituted derivative, paromycin and ribostamycin etc.; The destomycins of monosubstituted derivative) and amino-hexanol derivative (as sorbistin) etc.
At present, the clinical aminoglycoside antibiotics that uses is the derivative that contains 2-deoxystreptamine in the molecular structure more than 85%, and all lists China national essential drugs register mostly in, and most of gram-positive microorganisms and negative bacterium are had very strong anti-microbial effect.Occupy suitable share on market, the aminoglycoside antibiotics bulk drug that China produces accounts for 70% of world wide production.Therefore, the preparation method of research aminoglycoside antibiotics has certain directive significance and vast market prospect.
The aminoglycoside antibiotics that contains the 2-deoxystreptamine structure is a kind of water-soluble, polynary weakly alkaline microbiotic.Usually, contain 3~5 amino and 1~2 methylamino-in the molecule, in the aqueous solution, have multistage dissociation equilibrium.Therefore, aminoglycoside antibiotics can exist with different electrochemical states in the water solution system of different pH values, and the different valence state ion presents multistage dissociation equilibrium state, along with stepwise dissociation appears in the pH value of solution from high to low.During higher pH value, aminoglycoside antibiotics exists with the zeroth order form; During pH neutral, then exist with the low price cationic form; During acid ph value, then exist with the high-valence cationic form.
Because aminoglycoside antibiotics is polynary weakly alkaline biology organic bases, according to the ion-exchange theory, under acid and neutrallty condition, aminoglycoside antibiotics is to exist with the positively charged ion state, can select for use Zeo-karb to extract aminoglycoside antibiotics, but under alkaline condition, especially pH value>8.0 o'clock, aminoglycoside antibiotics mainly exists with molecularity, should select for use macroporous adsorbent resin to carry out separation and purification.
The main macroporous adsorbent resin commodity of domestic production circulation are divided macroporous adsorbent resin products such as then mainly containing polystyrene, acrylic acid series, phenolic aldehyde system, epoxy system, vinylpyridine system, urea aldehyde system and vinyl chloride according to the chemical constitution of resin matrix; Polarity according to resin can be divided into non-polar resin (as HZ802, HZ803, HZ816, HZ818, DA201, Amberlite XAD-2, Amberlite XAD-4,1300,1400), low-pole resin (as HZ801, HZ841, DK110, Amberlite IRC-84, D113), middle polarity resin (as HZ806, D101, AB-8) and polar resin macroporous adsorbent resin products such as (as NKA-9).
The pure product of aminoglycoside antibiotics mostly are white amorphous powder, are a kind of water-soluble organic-biological alkali, and molecular weight is about 400~800, is soluble in the hydrophilic solvents such as hot water and methyl alcohol, ethanol, is slightly soluble in chloroform, and is insoluble in sherwood oil, benzene; Its vitriol is soluble in water, is insoluble to organic solvent.Therefore, to being adsorbed on the aminoglycoside antibiotics alkaloid on the macroporous resin, can adopt hydrophilic solvents such as methyl alcohol, ethanol to carry out wash-out.
Summary of the invention
The object of the present invention is to provide the enriching and purifying macroporous resin process of aminoglycoside antibiotics, not only equipment is simple for this method, good separating effect, product yield height, and resin long service life, production cost are low.
The enriching and purifying macroporous resin process of aminoglycoside antibiotics of the present invention is achieved in that and will contains the solution of aminoglycoside antibiotics, under pH value 8.0~9.5 conditions, extremely saturated with absorption with macroporous adsorbent resin, debris and the more weak impurity of adsorptive power that elder generation is not adsorbed with water (V/V) flush away that is no less than 1.5 times of amount of resin, use 30~65% the hydrophilic solvent aqueous solution of 4~8 times of amount of resin (V/V) again, with 1~3 times of amount of resin/hour flow velocity (V/V) wash-out aminoglycoside antibiotics component, elutriant-0.06~-0.095MPa, under 50~65 ℃ of conditions, through decompression and solvent recovery, vacuum concentration, be spray dried to the aminoglycoside antibiotics product.
Hydrophilic solvent of the present invention preferentially selects for use the major advantage of methyl alcohol or acetone to be: though hydrophilic solvents such as methyl alcohol, acetone, ethanol, propyl alcohol or Virahol are suitable to the elute effect of aminoglycoside antibiotics, but for other hydrophilic solvent, the boiling point of methyl alcohol or acetone is lower, energy consumption is littler during solvent recuperation, can significantly reduce the preparation cost of aminoglycoside antibiotics product.
Preparation method's of the present invention major advantage is: made full use of macroporous adsorbent resin under pH value 8.0~9.5 conditions to different with to the adsorptive power of impurity such as protein, amino acid, polysaccharide, pigment, inorganic salt of the adsorptive power of targeted activity material aminoglycoside antibiotics, and the difference of impurity solubleness in the hydrophilic solvent aqueous solution such as aminoglycoside antibiotics and protein, amino acid, polysaccharide, pigment, inorganic salt, really reached the high efficiency separation of aminoglycoside antibiotics and impurity.
Embodiment
The enriching and purifying macroporous resin process of aminoglycoside antibiotics of the present invention, its key step is: the solution that contains aminoglycoside antibiotics, under pH value 8.0~9.5 conditions, extremely saturated with absorption with macroporous adsorbent resin, debris and the more weak impurity of adsorptive power that elder generation is not adsorbed with water (V/V) flush away that is no less than 1.5 times of amount of resin, use 30~65% the hydrophilic solvent aqueous solution of 4~8 times of amount of resin (V/V) again, with 1~3 times of amount of resin/hour flow velocity (V/V) wash-out aminoglycoside antibiotics component, elutriant-0.06~-0.095MPa, under 50~65 ℃ of conditions, through decompression and solvent recovery, vacuum concentration, be spray dried to the aminoglycoside antibiotics product.
Wherein, the solution that contains aminoglycoside antibiotics can be to derive from microorganism synthetic aminoglycoside antibiotics fermentating liquid filtrate filtrate, aminoglycoside antibiotics crystalline mother solution and aminoglycoside antibiotics extracting solution, elutriant or the destainer one or more.
Macroporous adsorbent resin can preferentially be selected HZ801, HZ802, HZ803, HZ806, HZ816, HZ818, HZ841, the Amberlite XAD-2 of polystyrene skeleton, among the Amberlite XAD-4 one or more for use, also can preferentially select D101, DK110, Amberlite IRC-84, the AB-8 of polyacrylic acid skeleton, among the D113 one or more for use.
Hydrophilic solvent can be one or more mixed solvents in methyl alcohol, acetone, ethanol, propyl alcohol or the Virahol, preferentially selects methyl alcohol or acetone for use.
Physical and chemical parameter measuring method of the present invention is as follows:
(1) aminoglycoside antibiotics Determination on content: adopting high performance liquid chromatography, is derivatization reagent with o-phthalaldehyde(OPA) and Thiovanic acid, and aminoglycoside antibiotics is carried out column front derivation.Condition determination: Agilent 1100 type high performance liquid chromatographs (DAD diode-array detector), Waters Nova-Pak C
18Chromatographic column (Φ 4.6 * 150mm, 5 μ m), moving phase is methyl alcohol: water: acetate (include sodium heptanesulfonate 12mmol/L, pH6.3)=70: 25: 5 (V/V/V), flow velocity 1.0ml/min, 30 ℃ of column temperatures, sample size 20 μ L detect wavelength 250nm.With various aminoglycoside antibiotics standard substance (purity 99%) (available from Sigma company) is contrast.
(2) determination of polysaccharide adopts the phenolsulfuric acid method, is contrast with glucose or D-semi-lactosi.Protein content determination adopts FoLin-phenol method, is contrast with the bSA.Inorganic ion content is measured and is adopted kit measurement, wherein SO
4 2-Mensuration adopt the bariumchloride precipitator method, Cl
-Measure and adopt the Silver Nitrate precipitator method, Ca
2+And Mg
2+Mensuration adopt methyl thymol blue complexometry.
(3) the spraying drying condition is: feeding liquid concentration 10~20 degree Beaume (60 ℃), 160~250 ℃ of PG-5 type spray-drier inlet temperatures, 60~110 ℃ of temperature outs, centrifugal head operating pressure 1.6~3.0kgf/cm
2
Preparation method's of the present invention embodiment is presented below:
Embodiment 1
The pH value is 7.36, micronomicin concentration is the fermentating liquid filtrate of 0.72g/L, behind sodium hydroxide solution adjust pH to 8.0, adopt the D101 macroporous adsorbent resin, with 2.0 times of amount of resin/hour last column flow rate (V/V), dynamic adsorption is to saturated, debris and the more weak impurity of adsorptive power that elder generation is not adsorbed with water (V/V) flush away that is no less than 1.5 times of amount of resin, use 48% methanol aqueous solution (V/V) of 4 times of amount of resin again, with 2 times of amount of resin/hour flow velocity (V/V) wash-out micronomicin component, elutriant-0.06~-0.095MPa, under 50~65 ℃ of conditions, through decompression and solvent recovery, vacuum concentration, be spray dried to the micronomicin product.After measured, the rate of recovery 90.5% of micronomicin, purity 76.5%.
Embodiment 2
The pH value is 8.32, tobramycin concentration is the extracting solution of 176.2g/L, with hydrochloric acid adjust pH to 8.0, adopt the AmberliteXAD-4 macroporous adsorbent resin, under the mixing speed condition of 75r/min, Static Adsorption is to saturated, debris and the more weak impurity of adsorptive power that elder generation is not adsorbed with water (V/V) flush away that is no less than 1.5 times of amount of resin, use 30% methanol aqueous solution (V/V) of 7 times of amount of resin again, with 3 times of amount of resin/hour flow velocity (V/V) wash-out tobramycin component, elutriant-0.06~-0.095MPa, under 50~65 ℃ of conditions, through decompression and solvent recovery, vacuum concentration, be spray dried to the tobramycin product.After measured, the rate of recovery 89.8% of tobramycin, purity 75.3%.
Embodiment 3
The pH value is 7.56, Xin Meisu concentration is the fermentating liquid filtrate of 12.9g/L, with sodium hydroxide solution adjust pH to 8.2, adopt Amberlite XAD-2 macroporous adsorbent resin, with 1.2 times of amount of resin/hour last column flow rate (V/V), dynamic adsorption is to saturated, debris and the more weak impurity of adsorptive power that elder generation is not adsorbed with water (V/V) flush away that is no less than 1.5 times of amount of resin, use 65% methanol aqueous solution (V/V) of 5 times of amount of resin again, with 1 times of amount of resin/hour flow velocity (V/V) wash-out Xin Meisu component, elutriant-0.06~-0.095MPa, under 50~65 ℃ of conditions, through decompression and solvent recovery, vacuum concentration, be spray dried to the Xin Meisu product.After measured, the rate of recovery 90.1% of Xin Meisu, purity 77.2%.
Embodiment 4
The pH value is 7.23, sisomicin concentration is the fermentating liquid filtrate of 1.1g/L, behind sodium hydroxide solution adjust pH to 9.3, adopt the HZ806 macroporous adsorbent resin, with 1.23 times of amount of resin/hour last column flow rate (V/V), dynamic adsorption is to saturated, debris and the more weak impurity of adsorptive power that elder generation is not adsorbed with water (V/V) flush away that is no less than 1.5 times of amount of resin, use 65% aqueous ethanolic solution (V/V) of 8 times of amount of resin again, with 3 times of amount of resin/hour flow velocity (V/V) wash-out sisomicin component, elutriant-0.06~-0.095MPa, under 50~65 ℃ of conditions, through decompression and solvent recovery, vacuum concentration, be spray dried to the sisomicin product.After measured, the rate of recovery 90.9% of sisomicin, purity 78.1%.
Embodiment 5
The pH value is 9.54, kantlex concentration is the crystalline mother solution of 257.2g/L, with hydrochloric acid soln adjust pH to 8.7, adopt (V/V) macroporous adsorbent resin of HZ801: HZ802 (2: 1), under the mixing speed condition of 75r/min, Static Adsorption is to saturated, debris and the more weak impurity of adsorptive power that elder generation is not adsorbed with water (V/V) flush away that is no less than 1.5 times of amount of resin, use the methyl alcohol of 49% concentration of 6 times of amount of resin again: ethanol (1: the 1) aqueous solution (V/V), with 1 times of amount of resin/hour flow velocity (V/V) wash-out kantlex component, elutriant-0.06~-0.095MPa, under 50~65 ℃ of conditions, through decompression and solvent recovery, vacuum concentration, be spray dried to the kantlex product.After measured, the rate of recovery 91.6% of kantlex, purity 78.3%.
Embodiment 6
The pH value is 7.58, kantlex concentration is the fermentating liquid filtrate of 8.0g/L, with sodium hydroxide solution adjust pH to 8.6, adopt the HZ816 macroporous adsorbent resin, under the mixing speed condition of 75r/min, Static Adsorption is to saturated, debris and the more weak impurity of adsorptive power that elder generation is not adsorbed with water (V/V) flush away that is no less than 1.5 times of amount of resin, use 30% aqueous ethanolic solution (V/V) of 8 times of amount of resin again, with 2 times of amount of resin/hour flow velocity (V/V) wash-out kantlex component, elutriant-0.06~-0.095MPa, under 50~65 ℃ of conditions, through decompression and solvent recovery, vacuum concentration, be spray dried to the kantlex product.After measured, the rate of recovery 89.0% of kantlex, purity 75.2%.
Embodiment 7
The pH value is 7.39, gentamicinC
1aConcentration is the fermentating liquid filtrate of 1.2g/L, behind sodium hydroxide solution adjust pH to 9.5, adopt the D113 macroporous adsorbent resin, with 1.8 times of amount of resin/hour last column flow rate (V/V), dynamic adsorption is to saturated, first debris and the more weak impurity of adsorptive power that is not adsorbed with water (V/V) flush away that is no less than 1.5 times of amount of resin is used 30% aqueous acetone solution (V/V) of 4 times of amount of resin again, with 1 times of amount of resin/hour flow velocity (V/V) wash-out gentamicinC
1aComponent, elutriant-0.06~-0.095MPa, 50~65 ℃ of conditions under, through decompression and solvent recovery, vacuum concentration, be spray dried to gentamicinC
1aProduct.After measured, gentamicinC
1aThe rate of recovery 90.8%, purity 76.09%.
Embodiment 8
The pH value is 7.38, ribostamycin concentration is the fermentating liquid filtrate of 2.0g/L, with sodium hydroxide solution adjust pH to 8.9, adopt the HZ801 macroporous adsorbent resin, under the mixing speed condition of 105r/min, Static Adsorption is to saturated, debris and the more weak impurity of adsorptive power that elder generation is not adsorbed with water (V/V) flush away that is no less than 1.5 times of amount of resin, use 65% aqueous acetone solution (V/V) of 4 times of amount of resin again, with 2 times of amount of resin/hour flow velocity (V/V) wash-out ribostamycin component, elutriant-0.06~-0.095MPa, under 50~65 ℃ of conditions, through decompression and solvent recovery, vacuum concentration, be spray dried to the ribostamycin product.After measured, the rate of recovery 90.4% of ribostamycin, purity 72%.
Embodiment 9
The pH value is 9.05, sisomicin concentration is the ammoniacal liquor elutriant of 186.7g/L, behind sodium hydroxide solution adjust pH to 9.5, adopt the HZ803 macroporous adsorbent resin, with 0.25 times of amount of resin/hour last column flow rate (V/V), dynamic adsorption is to saturated, debris and the more weak impurity of adsorptive power that elder generation is not adsorbed with water (V/V) flush away that is no less than 1.5 times of amount of resin, use the methyl alcohol of 55% concentration of 8 times of amount of resin again: acetone (3: the 1) aqueous solution (V/V), with 3 times of amount of resin/hour flow velocity (V/V) wash-out sisomicin component, elutriant-0.06~-0.095MPa, under 50~65 ℃ of conditions, through decompression and solvent recovery, vacuum concentration, vacuum spray drying becomes the sisomicin product.After measured, the rate of recovery 92.7% of sisomicin, purity 78.5%.
Above embodiment is intended to further describe for example the present invention, rather than limits the present invention by any way.
The present invention is novel, and technology is simple, the product quality height, and production cost is low, can be used for the further separation and purification of the aminoglycoside antibiotics solution of different sources, has bigger dissemination.
Claims (5)
1. the enriching and purifying macroporous resin process of an aminoglycoside antibiotics, it is characterized in that: the solution that will contain aminoglycoside antibiotics, in the pH value is under 8.0~9.5 conditions, extremely saturated with absorption with macroporous adsorbent resin, debris and the more weak impurity of adsorptive power that elder generation is not adsorbed with water (V/V) flush away that is no less than 1.5 times of amount of resin, use 30~65% the hydrophilic solvent aqueous solution of 4~8 times of amount of resin (V/V) again, with 1~3 times of amount of resin/hour flow velocity (V/V) wash-out aminoglycoside antibiotics component, elutriant-0.06~-0.095MPa, under 50~65 ℃ of conditions, through decompression and solvent recovery, vacuum concentration, be spray dried to the aminoglycoside antibiotics product.
2. the enriching and purifying macroporous resin process of aminoglycoside antibiotics according to claim 1, it is characterized in that: described spraying drying condition is: feeding liquid concentration 10~20 degree Beaume (60 ℃), 160~250 ℃ of PG-5 type spray-drier inlet temperatures, 60~110 ℃ of temperature outs, centrifugal head operating pressure 1.6~3.0kgf/cm
2
3. the enriching and purifying macroporous resin process of aminoglycoside antibiotics according to claim 1, it is characterized in that: described macroporous adsorbent resin skeleton is selected polystyrene or polyacrylic acid for use.
4. the enriching and purifying macroporous resin process of aminoglycoside antibiotics according to claim 1, it is characterized in that: described hydrophilic solvent is one or more mixed solvents in methyl alcohol, acetone, ethanol, propyl alcohol and the Virahol.
5. the enriching and purifying macroporous resin process of aminoglycoside antibiotics according to claim 1, it is characterized in that: the described solution that contains aminoglycoside antibiotics is one or more in microorganism synthetic aminoglycoside antibiotics fermentating liquid filtrate, aminoglycoside antibiotics crystalline mother solution and aminoglycoside antibiotics extracting solution, elutriant or the destainer.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CNB2006101353449A CN100447147C (en) | 2006-12-20 | 2006-12-20 | Aminoglycoside antibiotics enriching and purifying macroporous resin process |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CNB2006101353449A CN100447147C (en) | 2006-12-20 | 2006-12-20 | Aminoglycoside antibiotics enriching and purifying macroporous resin process |
Publications (2)
Publication Number | Publication Date |
---|---|
CN1974586A true CN1974586A (en) | 2007-06-06 |
CN100447147C CN100447147C (en) | 2008-12-31 |
Family
ID=38124973
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CNB2006101353449A Expired - Fee Related CN100447147C (en) | 2006-12-20 | 2006-12-20 | Aminoglycoside antibiotics enriching and purifying macroporous resin process |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN100447147C (en) |
Cited By (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102532213A (en) * | 2011-12-29 | 2012-07-04 | 福建和泉生物科技有限公司 | Method for preparing ribostamycin rough products by using membrane separation technology |
CN102617666A (en) * | 2012-03-07 | 2012-08-01 | 广州牌牌生物科技有限公司 | Process for making sisomicin content and micronomicin content of gentamicin sulphate consistent with standards in Chinese pharmacopoeia 2010 |
CN102796150A (en) * | 2012-08-31 | 2012-11-28 | 无锡福祈制药有限公司 | Method for separating and purifying high-purity sisomicin |
CN103193837A (en) * | 2012-01-06 | 2013-07-10 | 上海医药工业研究院 | Etimicin sulfate preparation method |
CN105732738A (en) * | 2016-03-09 | 2016-07-06 | 丽珠集团新北江制药股份有限公司 | Tobramycin purification method |
CN106076282A (en) * | 2016-06-07 | 2016-11-09 | 湖南大学 | A kind of resin combination and application thereof for adsorbing and/or neutralize antibiotic |
CN106946957A (en) * | 2017-05-11 | 2017-07-14 | 常州方圆制药有限公司 | The preparation method of Arbekacin intermediate dibekacin |
CN109438527A (en) * | 2018-09-20 | 2019-03-08 | 无锡济民可信山禾药业股份有限公司 | A method of recycling Gentamicin C1a from Etimicin sulfate intermediate synthesising by-product |
CN110563782A (en) * | 2019-09-29 | 2019-12-13 | 常州方圆制药有限公司 | Gentamicin C1aPurification method of (2) |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1055738A (en) * | 1990-04-16 | 1991-10-30 | 中外合资福州榕山生化制品开发有限公司 | Gibberellin extraction process |
CN1865273A (en) * | 2005-05-16 | 2006-11-22 | 厦门倍尔思生化科技有限公司 | Method for extracting multiple liquorice flavone form liquorice |
-
2006
- 2006-12-20 CN CNB2006101353449A patent/CN100447147C/en not_active Expired - Fee Related
Cited By (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102532213A (en) * | 2011-12-29 | 2012-07-04 | 福建和泉生物科技有限公司 | Method for preparing ribostamycin rough products by using membrane separation technology |
CN103193837A (en) * | 2012-01-06 | 2013-07-10 | 上海医药工业研究院 | Etimicin sulfate preparation method |
CN103193837B (en) * | 2012-01-06 | 2015-08-26 | 上海医药工业研究院 | Prepare the method for Etimicin sulfate |
CN102617666A (en) * | 2012-03-07 | 2012-08-01 | 广州牌牌生物科技有限公司 | Process for making sisomicin content and micronomicin content of gentamicin sulphate consistent with standards in Chinese pharmacopoeia 2010 |
CN102796150A (en) * | 2012-08-31 | 2012-11-28 | 无锡福祈制药有限公司 | Method for separating and purifying high-purity sisomicin |
CN105732738B (en) * | 2016-03-09 | 2018-11-09 | 丽珠集团新北江制药股份有限公司 | A kind of method of purification of tobramycin |
CN105732738A (en) * | 2016-03-09 | 2016-07-06 | 丽珠集团新北江制药股份有限公司 | Tobramycin purification method |
CN106076282A (en) * | 2016-06-07 | 2016-11-09 | 湖南大学 | A kind of resin combination and application thereof for adsorbing and/or neutralize antibiotic |
CN106076282B (en) * | 2016-06-07 | 2018-05-11 | 湖南大学 | A kind of resin combination and its application for being used to adsorbing and/or neutralizing antibiotic |
CN106946957A (en) * | 2017-05-11 | 2017-07-14 | 常州方圆制药有限公司 | The preparation method of Arbekacin intermediate dibekacin |
CN106946957B (en) * | 2017-05-11 | 2019-11-08 | 常州方圆制药有限公司 | The preparation method of Arbekacin intermediate |
CN109438527A (en) * | 2018-09-20 | 2019-03-08 | 无锡济民可信山禾药业股份有限公司 | A method of recycling Gentamicin C1a from Etimicin sulfate intermediate synthesising by-product |
CN110563782A (en) * | 2019-09-29 | 2019-12-13 | 常州方圆制药有限公司 | Gentamicin C1aPurification method of (2) |
CN110563782B (en) * | 2019-09-29 | 2022-08-30 | 常州方圆制药有限公司 | Gentamicin C 1a Purification method of (2) |
Also Published As
Publication number | Publication date |
---|---|
CN100447147C (en) | 2008-12-31 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN100447147C (en) | Aminoglycoside antibiotics enriching and purifying macroporous resin process | |
CN101012246B (en) | Ion exchange purifying method of aminoglycoside antibiotics | |
CN102718843B (en) | Preparation method of single teicoplanin components | |
US20160264620A1 (en) | Separation And Purification Method For Vancomycin Hydrochloride Of High Purity | |
CN103936837B (en) | Not Kangding B of a kind of purification knob0Method | |
CN102924572B (en) | Method for preparing high-purity daptomycin | |
CN112442096A (en) | High-purity Zhongshengmycin F reference substance and preparation method thereof | |
CN100463912C (en) | Membrane separating and purifying process for aminoglycoside antibiotics | |
US8173837B1 (en) | Process for the production of L-citrulline from watermelon flesh and rind | |
CN101921304B (en) | Method for purifying panaxoside compound-K by applying macroporous resin | |
CN101343295B (en) | Method for extracting francisella native from bykomycin fermentation liquor | |
CN1706858A (en) | Process for preparing jasminodin and genipin-1-beta-D-gentiobioside with cape jasmine fruit | |
CN103232525A (en) | Method for extracting polyoxin from streptomyces fermentation broth | |
CN102920727B (en) | Method for preparing extracts rich in vitexin rhamnoside and vitexin glucoside | |
CN111239314A (en) | Separation and analysis method of chitin oligosaccharide | |
CN100393691C (en) | Method for preparing mandelic acid by macroporous adsorptive resin | |
CN101591333B (en) | Method for purifying pseudomonas acid A | |
CN112409426B (en) | Preparation method of sisomicin sulfate | |
CN101838315B (en) | Method for separating Ramoplanin | |
CN111333687A (en) | Method for extracting franocidine sulfate from neomycin sulfate | |
CN113045611A (en) | Preparation method of high-purity lincomycin hydrochloride | |
CN1944451A (en) | Process for separating and purifying sisomicin using macro porous adsorption resin | |
CN100418979C (en) | Method of extracting sisomicin using cation exchange resin | |
CN102286051B (en) | Method for separating chenodeoxycholic acid from ursodesoxycholic acid | |
CN105669789A (en) | Preparation method for norvancomycin |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20081231 Termination date: 20151220 |
|
EXPY | Termination of patent right or utility model |