CN1969952A - Antivirus Chinese medicinal formulation, preparation process, quality control method and use thereof - Google Patents
Antivirus Chinese medicinal formulation, preparation process, quality control method and use thereof Download PDFInfo
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Abstract
The invention discloses a Chinese medicinal compound preparation which is prepared from baikal skullcap root and barrenwort, and can be prepared into injections, capsules, tablets, dispersible tablets, sublingual tablets, granules, oral liquid preparations, drop pills and other pharmacologically allowable dose forms. The invention can be applied to the treatment of pneumonia, respiratory tract infection, influenza, avian Influenza, SARS and asthma.
Description
Technical field
The present invention is a kind of anti virus herb preparation and preparation method thereof, quality control method and application, belongs to technical field of Chinese medicine.
Technical background
Flu is the upper respiratory tract infection that is caused by multiple virus, is commonly called as " cold ", is all generable common respiratory tract diseases of the four seasons.Viral influenza is a upper respiratory tract infection, is called for short again and goes up sense, and be the common respiratory infectious disease that causes by multiple virus.Though it is a lot of to be used for the treatment of the medicine of flu on the market, but the problem that the side effect of Western medicine ubiquity is big, and Chinese patent medicine is according to the different causes of disease, in conjunction with the medicine of theory of Chinese medical science in order to the treatment disease, for example anemofrigid cold, anemopyretic cold etc. all are to need to select different medicines to treat.And at present Western medicine class coldrex exists dizzy, drowsiness, myasthenia of limbs etc. allow the not drawback of fast response of people on the market; And pure Chinese medicine coldrex exists onset to wait shortcoming slowly.Better prevent and treat purpose in order to reach, a large amount of research has been done by many inventors and medicine enterprise, and some outstanding treatment products also are provided; As: number of patent application is: 200410048399.7, name is called the patent application of " a kind of antiviral oral cavity disintegration tablet and preparation method thereof ", and the disease that it is used for the flu aspect has rapid-action effect; But in further investigation, find, adopt the many big prescriptions of flavour of a drug, the complicated component of its preparation, and make patient's dosage big, on this basis the applicant to have adopted Radix Scutellariae, Herba Epimedii be medicine, reduced prescription, can not only reduce patient's dose, the employing reasonable process is removed the strong composition of some toxic and side effects in the medicinal substances extract, has improved the safety of preparation and the controllability of quality, greatly improve curative effect, and more help the molding of preparation.
Summary of the invention: the objective of the invention is to: a kind of antiviral Chinese medicine preparation and its production and application is provided; The present invention is directed to prior art, under Chinese medical theory instructs, on the basis of experiment screening, adopt Radix Scutellariae, Herba Epimedii compatibility to make preparation, optimize best prescription and technology; The product that obtains, particularly ejection preparation and sublingual lozenge formulation products can play the effect of the first pass effect that absorption is fast, bioavailability is high, rapid-action, avoid liver.
The present invention constitutes like this: calculate according to percentage by weight, it is by Radix Scutellariae 1～99%, Herba Epimedii 99～1%, and the extract of perhaps corresponding weight portion medical material adds suitable adjuvant and is made.Be preferably: calculate according to percentage by weight, it is by Radix Scutellariae 20～80%, Herba Epimedii 80～20%, and the extract of perhaps corresponding weight portion medical material adds suitable adjuvant and is made.Say accurately: calculate according to percentage by weight, it is by Radix Scutellariae 40～60%, Herba Epimedii 60～40%, and the extract of perhaps corresponding weight portion medical material adds suitable adjuvant and is made.
Described preparation can be any acceptable dosage form on the pharmaceutics: be directly used in the injection of drug administration by injection, directly supply the venous transfusion of intravenous drip, need be used for the concentrated solution for injection of intravenous drip and injectable sterile powder and aseptic block and tablet, capsule, granule, drop pill, pill, soft capsule, oral liquid, oral cavity disintegration tablet or the dispersible tablet that makes with freeze-drying or spray drying method after diluting.Say accurately: described preparation can be various injections, drop pill, sublingual lozenge, oral cavity disintegration tablet, dispersible tablet, soft capsule, capsule, ordinary tablet.
Contain flavones ingredient in the described preparation, wherein the flavones ingredient content in the injection is not less than in the preparation 25% of total solid after deduction adjuvant amount and the water quantities.
The preparation method of described anti virus herb preparation: get radix scutellariae medicinal materials, add water or ethanol extraction, extracting solution gets the Radix Scutellariae crude extract after carrying out suitably concentrating, and also can further adopt one or more methods mixing uses in alcohol deposition method, acid-base precipitation method, column chromatography, the solvent extraction to make with extra care to such an extent that Radix Scutellariae is made with extra care extract; Get epimedium herb, add water or ethanol extraction, extracting solution gets the Herba Epimedii crude extract after carrying out suitably concentrating, and also can further adopt one or more methods mixing uses in alcohol deposition method, column chromatography, the solvent extraction to make with extra care to such an extent that Herba Epimedii is made with extra care extract; Radix Scutellariae crude extract or refining extract are mixed with Herba Epimedii crude extract or refining extract, make different preparations with diverse ways behind the adding adjuvant.Described preparation can make on the basis that in Radix Scutellariae extract, the Herba Epimedii extract one or both is prepared into liposome or pro-liposome.
The preparation method of described anti virus herb preparation:
A, Radix Scutellariae extract are preparations like this: Radix Scutellariae is pulverized, and adds 5-10 times of volume 30-90% alcohol reflux 1-5 time, each 0.1-3 hour, merge extractive liquid, filters decompression filtrate recycling ethanol, concentrate, add 1-8 times of volume water dissolution, filter, filtrate is crossed macroporous resin column, earlier with 3-10 times of water flushing, reuse 30-90% alcohol desorption, collect stripping liquid, decompression recycling ethanol concentrates, and vacuum drying gets Radix Scutellariae extract;
B, Herba Epimedii extract are preparations like this: Herba Epimedii is cut into 0.5-2cm left and right sides segment, adds 5-15 times of volume 30-95% alcohol reflux 1-5 time, each 0.1-3 hour, merge extractive liquid, filters, and filtrate concentrates, add 1-8 times of water gaging, stirring and dissolving filters, filtrate is crossed polyamide column, earlier with 3-10 times of volume water washing, discards eluent, reuse 3-10 times of volume 30-95% ethanol elution collected eluent, decompression recycling ethanol, concentrate, vacuum drying gets Herba Epimedii extract; Extract is mixed, make different preparations with diverse ways behind the adding adjuvant.
Described pharmaceutical adjunct can be one or more in lactose, galactose, mannitol, corn starch, polyvinylpyrrolidone K30, citric acid, sodium citrate, magnesium stearate, pigment, acetoacetate derivative, sweeting agent, spice, glycine, glucose, sodium chloride, dextran, glycerol, ethanol, propylene glycol, Polyethylene Glycol, sorbitol, tween, HP-, the poloxamer.
Injectable sterile block in the described preparation prepares like this: get Radix Scutellariae, the extract of Herba Epimedii, press medicine and added mannitol than 1: 2.2 with supplementary product consumption, add 1800ml water for injection, stirring makes dissolving, with saturated sodium hydroxide solution adjust pH to 6.0～7.0, add the injection water to 2000ml, mixing, the needle-use activated carbon of adding 0.3%, boiled 30 minutes, coarse filtration, reuse 0.45 μ m and 0.22 μ m microporous filter membrane filter, the filtrate packing, every bottle of 2.0ml, lyophilization, the equilibration time when the balance solidification point of phase I is 0 ℃ is 3 hours, i.e. the time of shelf temperature and product temperature basically identical; The second stage solidification point is from 0 ℃ during to minimum eutectic temperature-18 ℃, and shelf temperature and product temperature equilibration time are 2 hours; Phase III continues to be cooled to-60 ℃, needs 2 hours approximately, keeps this temperature 3 hours, freeze the jail fully until product, promptly begin evacuation, enter drying program, evacuation under-65 ℃ of constant temperature slowly heats up 2～3 ℃/h, to the lowest total of the melting point temperature, the time is about 8 hours, after sublimation drying is finished, continuation is under the low pressure condition, and it is dry to remove residual moisture to heat up, and the time is about 10～12 hours, kept more than 30 ℃ dry 5 hours, gland promptly gets the Injectable sterile block.
Described sublingual lozenge is such preparation: the extract of getting Radix Scutellariae, Herba Epimedii mixes, and adds 15% lactose, 20% corn starch, 15% polyvinylpyrrolidone K30, crosses 30 mesh sieves, add an amount of Pulvis Talci, micropowder silica gel, evenly mixed, tabletting promptly gets sublingual lozenge.The adjuvant of sublingual lozenge can be following one or more: lactose, mannitol, corn starch, polyvinylpyrrolidone K30, citric acid, sodium citrate, magnesium stearate, xanthein, acetoacetate derivative, sweeting agent, lemon flavouring.
Pro-liposome in the described preparation prepares like this: get in Radix Scutellariae extract, the Herba Epimedii extract one or both, add the phosphate buffer dissolving, add in the fused solution of fabaceous lecithin, cholesterol and 18-amine. mixing, stir supersound process, freezing, the dry powder that obtains sieves, promptly.
Described method of quality control comprise in the following method partly or entirely:
(1) finger printing of anti-viral pharmaceutical compositions test comprises based on the finger printing of Radix Scutellariae composition characteristics with based in the finger printing of Herba Epimedii composition characteristics one or more;
(2) radix scutellariae medicinal materials, epimedium herb, baicalin, noroxylin, chrysin, icariin, icariside I, Herba Epimedii glycosides A, B, C, precious icariin I, II, the content test method of all or part of composition in VI, the Quercetin;
(3) baicalin, noroxylin, chrysin, icariin, icariside I, Herba Epimedii glycosides A, B, C, precious icariin I, II, the content test method of all or part of composition in VI, Quercetin, the total flavones;
Described preparation is used to prepare the medicine of diseases such as treatment pneumonia, respiratory tract infection, influenza, bird flu, SARS, asthma, human body immunity improving power.
For proving that medicine provided by the invention has effective effect, the applicant has carried out a series of experiments;
Experimental example 1: to the comparative study of different proportioning pharmacodynamics
By the experiment of Diplococcus pneumoniae infecting mouse protective effect, the prescription of different proportion has been carried out the screening test of system, wherein: get 18～22gKM kind mice random packet, 21 every group, 1. the male and female dual-purpose fills a prescription 1: blank group equivalent normal saline; 2. fill a prescription 2: model control group, equivalent normal saline; 3. fill a prescription 3: Radix Scutellariae extract/Herba Epimedii extract=99/1; 4. fill a prescription 4: Radix Scutellariae extract/Herba Epimedii extract=80/20; 5. fill a prescription 5: Radix Scutellariae extract/Herba Epimedii extract=40/60; 6. fill a prescription 6: Radix Scutellariae extract/Herba Epimedii extract=60/40; 7. fill a prescription 7: Radix Scutellariae extract/Herba Epimedii extract=20/80; 8. fill a prescription 8: Radix Scutellariae extract/Herba Epimedii extract=1/99.Each organizes mice by administration in 1 time/day * 6 days, and administration in the 3rd day is after 1 hour, and 0.5ml/ Mus of lumbar injection Diplococcus pneumoniae bacterium liquid (except that the blank group) continues administration 3 days, observes death condition in each treated animal 7 days.The results are shown in Table 1:
Table 1 prescription research conclusion
| Formula number | Number of animals (only) | Death toll (only) | Survival rate (%) |
| 1 2 3 4 5 6 7 8 | 21 21 21 21 21 21 21 21 | 0 19 16 12 9 10 14 15 | 100 9.5 23.8 42.9 57.1 52.4 33.3 28.6 |
By experimental result as can be known, Radix Scutellariae extract: best compatibility scope=40～60%: 60～40% of Herba Epimedii extract.
Experimental example 2: Study on Forming
2.1 the selection of injection filler kind and consumption
The applicant finds that in development suitable filler, filler loading are powder injection formulation molding and stable key factor, and in order to improve the quality of this powder injection formulation, at first, the applicant investigates the kind of filler.The results are shown in following table.
2.1.1 different filler kind screenings
The different filler lyophilizing of table 2 effect relatively
| Filler | Mannitol | Glucose | Lactose | Sodium chloride | HP-β-CD |
| Medicine: adjuvant mouldability | 1: 2.2 good | Not molding in 1: 2.2 | Not molding in 1: 2.2 | Not molding in 1: 2.2 | Not molding in 1: 2.2 |
As seen, because the eutectic point height of mannitol, good water solubility be beneficial to the lyophilizing and the molding of this product, and other filler all is not suitable for this product, so selection mannitol is filler.
2.1.2 the screening of mannitol consumption
The applicant investigates the weight ratio of mannitol and effective ingredient then, the results are shown in following table.
The different filler proportionings of table 3 are to the influence of lyophilizing effect
| Medicine: mannitol | Color | Mouldability | Water solublity | Clarity |
| 1∶1 1∶1.4 1∶1.8 1∶2.2 1∶2.5 | Yellow faint yellow yellowish white | The difference difference is good carefully | The difference difference is good carefully | Difference difference difference good job |
As seen, medicine: mannitol=1: 2.2 o'clock, formed product is effective, considers from economic angle, determines medicine: mannitol=1: 2.2.
2.2 the selection of sublingual lozenge filler kind and consumption
2.2.1 different filler kind screenings
Sublingual lozenge then is directly to be absorbed and the tablet medicine of performance general action by the Sublingual mucosa.Sublingual lozenge can prevent that gastrointestinal tract from destroying the active ingredient of medicine, also can avoid the drug influence liver function.It is poor to have solved former dosage form disintegrative, and stripping is shortcoming slowly.
Investigation factor and level: pass through preliminary experiment, the rapid release absorption and the curative effect of the viral prescription of antagonism are had the clearly factor of influence: lactose powder (A) corn starch (B) and polyvinylpyrrolidone K30 (C) and consumption are investigated as variable factor, and each factor is fetched water to put down and seen the following form.Select L9 (4 for use
3) orthogonal table experimentizes.
Table 4 orthogonal test factor level table
| Lactose | Corn starch | Polyvinylpyrrolidone K30 | |
| 1 2 3 | 20％ 15％ 5％ | 20％ 15％ 10％ | 20％ 15％ 10％ |
With to the survival rate of infection of staphylococcus aureus mice as index, concrete experiment is: get 18～22g, KM kind mice random packet, every group 21, the male and female dual-purpose, each organized administration 1 time/day * 6 days, administration was after 1 hour in the 3rd day, 0.5ml/ Mus of lumbar injection staphylococcus aureus bacterium liquid continued administration 3 days, observed death condition in each treated animal 7 days.Experiment is divided into groups and be the results are shown in following table
Arrangement of table 5 orthogonal test and result
| The factor group number | Lactose | Mannitol | Polyvinylpyrrolidone K30 | Survival rate (%) |
| 1 2 3 4 5 6 7 8 9 | 1 1 1 2 2 2 3 3 3 | 1 2 3 1 2 3 1 2 3 | 1 2 3 2 3 1 3 1 2 | 9.5 19.0 28.6 33.3 19.0 23.8 23.8 19.0 14.3 |
As seen, adjuvant is selected 15% lactose, 20% corn starch, 15% polyvinylpyrrolidone K30, product good drug efficacy for use.
Experimental example 3: preparation pharmacodynamic experiment
3.1 vivo bacteria corrosion action
Protective effect to the infection of staphylococcus aureus mice: get 18～22g, KM kind mice random packet, 21 every group, male and female dual-purpose, 1. blank group equivalent normal saline; 2. model control group, the equivalent normal saline; 3. antiviral granule 3g/kg; 4. injection group 3g/kg of the present invention; 5. sublingual lozenge group 3g/kg of the present invention.Below respectively organize administration 1 time/day * 6 days, administration in the 3rd day is after 1 hour, and 0.5ml/ Mus of lumbar injection staphylococcus aureus bacterium liquid (except that the blank group) continues administration 3 days, observes death condition in each treated animal 7 days, the results are shown in Table.
Table 6 anti-virus formulation is to the protective effect of infection of staphylococcus aureus mice
| Group | Dosage (g/kg) | Number of animals (only) | Death toll (only) | Survival rate (%) |
| Blank group model matched group antiviral granule injection group of the present invention sublingual lozenge group of the present invention | - - 3.00 3.00 3.00 | 21 21 21 21 21 | 0 17 14 11 13 | 100 19.0 33.3 47.6 38.1 |
By experimental result as can be known, than the antiviral granule height, pharmaceutical preparation of the present invention has inhibitory action to staphylococcus aureus to the survival rate of infection of staphylococcus aureus mice in pharmaceutical preparation of the present invention.
3.2 interior resisting virus experiment
The interior resisting virus effect: virus infected mice median lethal dose(LD 50) (LD50) is measured and is got 49 of KM kind mices, body weight 11～13g, the male and female dual-purpose is divided into 7 groups at random, every group 7, get and increase 3 times viral allantoic fluid of poison, with 10 times of dilutions, every group drips a virus concentration, each organize mice under the ether light anaesthesia with viral allantoic fluid 30ul collunarium, observe death toll in 14 days, press Reed Muench method and calculate, LD50 is 2.88.
Protective effect to the influenza a virus infection mice: get KM kind mice, body weight 11～13g, male and female half and half are divided in groups 21 every group (1) blank groups, equivalent normal saline at random; (2) model control group, the equivalent normal saline; (3) ribavirin group 75mg/kg; (4) injection group 3g/kg of the present invention; (5) sublingual lozenge group 3g/kg of the present invention.Below respectively organize mice administration 1 time/* 6 days; in administration respectively organized in the day mice (except that the blank group) under the ether light anaesthesia to increase the viral allantois drop nose infecting mouse of poison 3 times; every Mus 30ul (20 LD50 challenging doses) observes zoogenetic infection sequela symptom; record infects death toll after 14 days; and calculate protective rate and average survival natural law, the results are shown in Table
Table 7 anti-virus formulation is to the protective effect of influenza a virus infection mice
| Group | Dosage (g/kg) | Number of animals (only) | Death toll (only) | Survival rate (%) |
| Blank group model matched group ribavirin group injection group of the present invention sublingual lozenge group of the present invention | - - 0.75 3.00 3.00 | 21 21 21 21 21 | 0 19 3 8 10 | 100 9.5 85.7 61.9 52.4 |
By experimental result as can be known, pharmaceutical preparation of the present invention has obvious protective effect to the influenza a virus infection mice, and pharmaceutical preparation of the present invention has inhibitory action better to influenza A virus.
Concrete embodiment
Embodiments of the invention 1: Radix Scutellariae 50g Herba Epimedii 50g
The preparation method of A, Radix Scutellariae extract is: Radix Scutellariae is pulverized, add 8 times of volume 70% alcohol reflux 3 times, each 1 hour, merge extractive liquid,, filter, decompression filtrate recycling ethanol, measuring relative density when being concentrated into 60 ℃ is 1.05～1.15, adds 3 times of volume water dissolutioies, filter, filtrate is crossed the AB-8 macroporous resin column, earlier with 6 times of water flushings, reuse 60% alcohol desorption, collect stripping liquid, decompression recycling ethanol, measuring relative density when being concentrated into 60 ℃ is 1.05～1.15, vacuum drying gets Radix Scutellariae extract.
The preparation method of B, Herba Epimedii extract is: Herba Epimedii is cut into 1cm left and right sides segment, add 10 times of volume 70% alcohol reflux 3 times, each 1 hour, merge extractive liquid,, filter, measuring relative density when filtrate is condensed into 50 ℃ is 1.05～1.15, adds 3 times of water gagings, stirring and dissolving, filter, filtrate is crossed polyamide column, earlier with 8 times of volume water washings, discard eluent, 8 times of volume 70% ethanol elutions of reuse are collected eluent, decompression recycling ethanol, measuring relative density when being condensed into 50 ℃ is 1.10～1.20, and vacuum drying gets Herba Epimedii extract.
Get the extract of Radix Scutellariae, Herba Epimedii, press medicine and added mannitol than 1: 2.2 with supplementary product consumption, add 1800ml water for injection, stirring makes dissolving, with saturated sodium hydroxide solution adjust pH to 6.0～7.0, add the injection water to 2000ml, mixing, the needle-use activated carbon of adding 0.3%, boiled 30 minutes, coarse filtration, reuse 0.45 μ m and 0.22 μ m microporous filter membrane filter, the filtrate packing, every bottle of 2.0ml, lyophilization, the equilibration time when the balance solidification point of phase I is 0 ℃ is 3 hours, i.e. the time of shelf temperature and product temperature basically identical; The second stage solidification point is from 0 ℃ during to minimum eutectic temperature-18 ℃, and shelf temperature and product temperature equilibration time are 2 hours; Phase III continues to be cooled to-40 ℃, needs 2 hours approximately, keeps this temperature 3 hours, freezes the jail fully until product, promptly begin evacuation, enter drying program, slowly heat up 2～3 ℃/h, to the lowest total of the melting point temperature, the time is about 8 hours, after sublimation drying is finished, continuation is under the low pressure condition, and it is dry to remove residual moisture to heat up, and the time is about 10～12 hours, kept more than 30 ℃ dry 5 hours, gland promptly gets the Injectable sterile block, intravenous injection, once-a-day, one time one.Content of total flavone accounts for 28% of total solid after deduction adjuvant amount and the water quantities in the said preparation.
Embodiments of the invention 2: Radix Scutellariae 50g Herba Epimedii 50g
The preparation method of A, Radix Scutellariae extract is: Radix Scutellariae is pulverized, add 8 times of volume 70% alcohol reflux 3 times, each 1 hour, merge extractive liquid,, filter, decompression filtrate recycling ethanol, measuring relative density when being concentrated into 60 ℃ is 1.05～1.15, adds 3 times of volume water dissolutioies, filter, filtrate is crossed the AB-8 macroporous resin column, earlier with 6 times of water flushings, reuse 60% alcohol desorption, collect stripping liquid, decompression recycling ethanol, measuring relative density when being concentrated into 60 ℃ is 1.05～1.15, vacuum drying gets Radix Scutellariae extract.
The preparation method of B, Herba Epimedii extract is: Herba Epimedii is cut into 1cm left and right sides segment, add 10 times of volume 70% alcohol reflux 3 times, each 1 hour, merge extractive liquid,, filter, measuring relative density when filtrate is condensed into 50 ℃ is 1.05～1.15, adds 3 times of water gagings, stirring and dissolving, filter, filtrate is crossed polyamide column, earlier with 8 times of volume water washings, discard eluent, 8 times of volume 70% ethanol elutions of reuse are collected eluent, decompression recycling ethanol, measuring relative density when being condensed into 50 ℃ is 1.10～1.20, and vacuum drying gets Herba Epimedii extract.
The extract of getting Radix Scutellariae, Herba Epimedii mixes, and adds 15% lactose, 20% corn starch, 15% polyvinylpyrrolidone K30, crosses 30 mesh sieves, adds an amount of Pulvis Talci, micropowder silica gel, and evenly mixed, tabletting promptly gets sublingual lozenge.General flavone content is 54% in the said preparation.
Embodiments of the invention 3: Radix Scutellariae 40g Herba Epimedii 60g
The preparation method of A, Radix Scutellariae extract is: Radix Scutellariae is pulverized, add 8 times of volume 70% alcohol reflux 3 times, each 1 hour, merge extractive liquid,, filter, decompression filtrate recycling ethanol, measuring relative density when being concentrated into 60 ℃ is 1.05～1.15, adds 3 times of volume water dissolutioies, filter, filtrate is crossed the AB-8 macroporous resin column, earlier with 6 times of water flushings, reuse 60% alcohol desorption, collect stripping liquid, decompression recycling ethanol, measuring relative density when being concentrated into 60 ℃ is 1.05～1.15, vacuum drying gets Radix Scutellariae extract.
The preparation method of B, Herba Epimedii extract is: Herba Epimedii is cut into 1cm left and right sides segment, add 10 times of volume 70% alcohol reflux 3 times, each 1 hour, merge extractive liquid,, filter, measuring relative density when filtrate is condensed into 50 ℃ is 1.05～1.15, adds 3 times of water gagings, stirring and dissolving, filter, filtrate is crossed polyamide column, earlier with 8 times of volume water washings, discard eluent, 8 times of volume 70% ethanol elutions of reuse are collected eluent, decompression recycling ethanol, measuring relative density when being condensed into 50 ℃ is 1.10～1.20, and vacuum drying gets Herba Epimedii extract.
Get the extract of Radix Scutellariae, Herba Epimedii, press medicine and added mannitol than 1: 2.2 with supplementary product consumption, add 1800ml water for injection, stirring makes dissolving, with saturated sodium hydroxide solution adjust pH to 6.0～7.0, add the injection water to 2000ml, mixing, the needle-use activated carbon of adding 0.3%, boiled 30 minutes, coarse filtration, reuse 0.45 μ m and 0.22 μ m microporous filter membrane filter, the filtrate packing, every bottle of 2.0ml, lyophilization, the equilibration time when the balance solidification point of phase I is 0 ℃ is 3 hours, i.e. the time of shelf temperature and product temperature basically identical; The second stage solidification point is from 0 ℃ during to minimum eutectic temperature-18 ℃, and shelf temperature and product temperature equilibration time are 2 hours; Phase III continues to be cooled to-45 ℃, needs 2 hours approximately, keeps this temperature 3 hours, freeze the jail fully until product, promptly begin evacuation, enter drying program, slowly heat up, 2～3 ℃/h, to the lowest total of the melting point temperature, time is about 8 hours, after sublimation drying is finished, continues under the low pressure condition, heating up, dry the time is about 10～12 hours to remove residual moisture, keeps more than 30 ℃ dry 5 hours, gland promptly gets the Injectable sterile block.
Embodiments of the invention 4: Radix Scutellariae 60g Herba Epimedii 40g
The preparation method of A, Radix Scutellariae extract is: Radix Scutellariae is pulverized, add 8 times of volume 70% alcohol reflux 3 times, each 1 hour, merge extractive liquid,, filter, decompression filtrate recycling ethanol, measuring relative density when being concentrated into 60 ℃ is 1.05～1.15, adds 3 times of volume water dissolutioies, filter, filtrate is crossed the AB-8 macroporous resin column, earlier with 6 times of water flushings, reuse 60% alcohol desorption, collect stripping liquid, decompression recycling ethanol, measuring relative density when being concentrated into 60 ℃ is 1.05～1.15, vacuum drying gets Radix Scutellariae extract.
The preparation method of B, Herba Epimedii extract is: Herba Epimedii is cut into 1cm left and right sides segment, add 10 times of volume 70% alcohol reflux 3 times, each 1 hour, merge extractive liquid,, filter, measuring relative density when filtrate is condensed into 50 ℃ is 1.05～1.15, adds 3 times of water gagings, stirring and dissolving, filter, filtrate is crossed polyamide column, earlier with 8 times of volume water washings, discard eluent, 8 times of volume 70% ethanol elutions of reuse are collected eluent, decompression recycling ethanol, measuring relative density when being condensed into 50 ℃ is 1.10～1.20, and vacuum drying gets Herba Epimedii extract.
The extract of getting Radix Scutellariae, Herba Epimedii mixes, in adding lactose, mannitol, corn starch, polyvinylpyrrolidone K30, citric acid, sodium citrate, magnesium stearate, xanthein, acetoacetate derivative, sweeting agent, the lemon flavouring one or more, cross 30 mesh sieves, add an amount of Pulvis Talci, micropowder silica gel, evenly mixed, tabletting promptly gets sublingual lozenge.
Embodiments of the invention 5: Radix Scutellariae 70g Herba Epimedii 30g
The preparation method of A, Radix Scutellariae extract is: Radix Scutellariae is pulverized, and adds 8 times of volume 70% alcohol reflux 3 times, each 1 hour, merge extractive liquid, filters decompression filtrate recycling ethanol, measuring relative density when being concentrated into 60 ℃ is 1.05～1.15, adds 3 times of volume water dissolutioies, filters, filtrate stirs with hydrochloric acid adjust pH to 2, and placement is spent the night, filter, precipitation washes with water, drains, and drying under reduced pressure gets Radix Scutellariae extract.
B, the preparation method of Herba Epimedii extract is: Herba Epimedii is cut into 1cm left and right sides segment, add 10 times of volume 70% alcohol reflux 3 times, each 1 hour, merge extractive liquid,, filter, measuring relative density when filtrate is condensed into 50 ℃ is 1.05～1.15, adds 3 times of water gagings, stirring and dissolving, filter, filtrate is crossed polyamide column, earlier with 8 times of volume water and 5 times of volume 10% washing with alcohol, discard eluent, 8 times of volume 60% ethanol elutions of reuse are collected eluent, decompression recycling ethanol, measuring relative density when being condensed into 50 ℃ is 1.10～1.20, and vacuum drying gets Herba Epimedii extract.
Get above extract and add an amount of water for injection dissolving, add the glucose or the sodium chloride of ormal weight, by volume add 0.3% active carbon behind the mixed dissolution, boil, keep little 30min that boils, cold slightly filtration, filtrate adds the injection water to ormal weight, boil the saturated sodium hydroxide solution of reuse adjust pH to 6.0～6.5, and 4 ℃ of cold preservations are spent the night, coarse filtration, fine straining, add the injection water, packing, sterilization promptly gets glucose or sodium chloride intravenous infusion.
Embodiments of the invention 6: Radix Scutellariae 80g Herba Epimedii 20g
The preparation method of A, Radix Scutellariae extract is: Radix Scutellariae is pulverized, add 8 times of volume 70% alcohol reflux 3 times, each 1 hour, merge extractive liquid,, filter, decompression filtrate recycling ethanol, measuring relative density when being concentrated into 60 ℃ is 1.05～1.15, adds 3 times of volume water dissolutioies, filter, filtrate is crossed the AB-8 macroporous resin column, earlier with 6 times of water and 5 times of 15% alcohol flushing, discards eluent; Reuse 60% alcohol desorption is collected stripping liquid, decompression recycling ethanol, and measuring relative density when being concentrated into 60 ℃ is 1.05～1.15, vacuum drying gets Radix Scutellariae extract.
B, the preparation method of Herba Epimedii extract is: Herba Epimedii is cut into 1cm left and right sides segment, add 10 times of volume 70% alcohol reflux 3 times, each 1 hour, merge extractive liquid,, filter, measuring relative density when filtrate is condensed into 50 ℃ is 1.05～1.15, adds 3 times of water gagings, stirring and dissolving, filter, filtrate is crossed the D-101 macroporous resin column, earlier with 8 times of volume water and 5 times of volume 25% washing with alcohol, discard eluent, 6 times of volume 50% ethanol elutions of reuse are collected eluent, decompression recycling ethanol, measuring relative density when being condensed into 50 ℃ is 1.10～1.20, and vacuum drying gets Herba Epimedii extract.
Above extract is mixed evenly, add 2.5 times of amount dextrin, 0.4% stevioside, 1.5% microcrystalline Cellulose with an amount of alcoholic solution system soft material, is granulated, and 65 ℃ of forced air dryings are granulated, and granulate promptly gets granule.
Embodiments of the invention 7: Radix Scutellariae 90g Herba Epimedii 10g
The preparation method of A, Radix Scutellariae extract is: Radix Scutellariae is pulverized, add 8 times of volume 70% alcohol reflux 3 times, each 1 hour, merge extractive liquid,, filter, decompression filtrate recycling ethanol, measuring relative density when being concentrated into 60 ℃ is 1.05～1.15, adds 3 times of volume water dissolutioies, filter, filtrate is crossed the AB-8 macroporous resin column, earlier with 6 times of water and 5 times of 20% alcohol flushing, discards eluent; Reuse 60% alcohol desorption is collected stripping liquid, decompression recycling ethanol, and measuring relative density when being concentrated into 60 ℃ is 1.0～1.05, with hydrochloric acid adjust pH to 2, stirs, and placement is spent the night, and filters, and precipitation washes with water, drains, and vacuum drying gets Radix Scutellariae extract.
The preparation method of B, Herba Epimedii extract is: Herba Epimedii is cut into 1cm left and right sides segment, add 10 times of volume 70% alcohol reflux 3 times, each 1 hour, merge extractive liquid, filters, and measuring relative density when filtrate is condensed into 50 ℃ is 1.05～1.15, add 4 times of volume water stirring and dissolving, filter, measuring relative density when filtrate is condensed into 50 ℃ is 1.10～1.20, and vacuum drying gets Herba Epimedii extract.
Above extract is mixed evenly, add the dextrin of equivalent, mix homogeneously is granulated, and is encapsulated, promptly gets capsule.
Embodiments of the invention 8: Radix Scutellariae 99g Herba Epimedii 1g
The preparation method of A, Radix Scutellariae extract is: Radix Scutellariae is pulverized, and adds 5 times of volume 30% alcohol reflux 1 time, each 0.1 hour, merge extractive liquid, filters decompression filtrate recycling ethanol, concentrate, add 1 times of volume water dissolution, filter, filtrate is crossed macroporous resin column, earlier with 3 times of water flushings, reuse 30% alcohol desorption, collect stripping liquid, decompression recycling ethanol concentrates, and vacuum drying gets Radix Scutellariae extract;
The preparation method of B, Herba Epimedii extract is: Herba Epimedii is cut into 0.5cm left and right sides segment, adds 5 times of volume 30% alcohol reflux 1 time, each 0.1 hour, merge extractive liquid, filters, and filtrate concentrates, add 1 times of water gaging, stirring and dissolving filters, filtrate is crossed polyamide column, earlier with 3 times of volume water washings, discards eluent, 3 times of volume 30% ethanol elutions of reuse are collected eluent, decompression recycling ethanol, concentrate, vacuum drying gets Herba Epimedii extract;
Above extract is mixed evenly, add distilled water, filter repeatedly, till the filtrate clarification., filter with absorbent cotton after the stirring and dissolving in filtrate with sucrose, it is an amount of to add distilled water on filter, shakes up, and promptly gets syrup.
Embodiments of the invention 9: Radix Scutellariae 1g Herba Epimedii 99g
The preparation method of A, Radix Scutellariae extract is: Radix Scutellariae is pulverized, and adds 10 times of volume 90% alcohol reflux 5 times, each 3 hours, merge extractive liquid, filters decompression filtrate recycling ethanol, concentrate, add 8 times of volume water dissolutioies, filter, filtrate is crossed macroporous resin column, earlier with 10 times of water flushings, reuse 90% alcohol desorption, collect stripping liquid, decompression recycling ethanol concentrates, and vacuum drying gets Radix Scutellariae extract;
The preparation method of B, Herba Epimedii extract is: Herba Epimedii is cut into 2cm left and right sides segment, adds 15 times of volume 95% alcohol reflux 5 times, each 3 hours, merge extractive liquid, filters, and filtrate concentrates, add 8 times of water gagings, stirring and dissolving filters, filtrate is crossed polyamide column, earlier with 10 times of volume water washings, discards eluent, 10 times of volume 95% ethanol elutions of reuse are collected eluent, decompression recycling ethanol, concentrate, vacuum drying gets Herba Epimedii extract;
Above extract is mixed evenly, be dissolved in the phosphate buffer (0.15M) standby, a certain proportion of fabaceous lecithin, cholesterol are dissolved in the 18-amine. solution, add in the phosphate-buffered liquor of said medicine, the water-bath type Ultrasound Instrument is handled 10min, gets liposome turbid liquor, behind the frozen drying, cross 180 mesh sieves, aseptic subpackaged, promptly get the pro-liposome injectable powder.
Embodiments of the invention 10: Radix Scutellariae 10g Herba Epimedii 90g
The preparation method of A, Radix Scutellariae extract is: Radix Scutellariae is pulverized, and adds 8 times of volume 70% alcohol reflux 3 times, each 1 hour, merge extractive liquid, filters decompression filtrate recycling ethanol, measuring relative density when being concentrated into 60 ℃ is 1.05～1.15, adds 3 times of volume water dissolutioies, filters, filtrate stirs with hydrochloric acid adjust pH to 2, and placement is spent the night, filter, precipitation washes with water, drains, and drying under reduced pressure gets Radix Scutellariae extract.
B, the preparation method of Herba Epimedii extract is: Herba Epimedii is cut into 1cm left and right sides segment, add 10 times of volume 70% alcohol reflux 3 times, each 1 hour, merge extractive liquid,, filter, measuring relative density when filtrate is condensed into 50 ℃ is 1.05～1.15, adds 3 times of water gagings, stirring and dissolving, filter, filtrate is crossed the D-101 macroporous resin column, earlier with 8 times of volume water and 5 times of volume 25% washing with alcohol, discard eluent, 6 times of volume 50% ethanol elutions of reuse are collected eluent, decompression recycling ethanol, measuring relative density when being condensed into 50 ℃ is 1.10～1.20, and vacuum drying gets Herba Epimedii extract.
Above extract is mixed evenly, will be that 1.8: 1 sodium stearate is put in the rustless steel container with the principal agent ratio, add extract, mix homogeneously is heated to 80-85 ℃, treat whole fusions after, 70-75 ℃ of insulation, mechanical high-speed are stirred 15min to evenly, are transferred in the reservoir, the dropping liquid valve is regulated in 70～75 ℃ of insulations, splashes in 30～35 ℃ the kerosene, drip apart from 5～6cm, drip 40～45 droplets/minute of speed, to the greatest extent and wipe kerosene the drop pill drop that forms, packing promptly gets drop pill.
Embodiments of the invention 11: Radix Scutellariae 20g Herba Epimedii 80g
The preparation method of A, Radix Scutellariae extract is: Radix Scutellariae is pulverized, add 8 times of volume 70% alcohol reflux 3 times, each 1 hour, merge extractive liquid,, filter, decompression filtrate recycling ethanol, measuring relative density when being concentrated into 60 ℃ is 1.05～1.15, adds 3 times of volume water dissolutioies, filter, filtrate is crossed the AB-8 macroporous resin column, earlier with 6 times of water and 5 times of 15% alcohol flushing, discards eluent; Reuse 60% alcohol desorption is collected stripping liquid, decompression recycling ethanol, and measuring relative density when being concentrated into 60 ℃ is 1.05～1.15, vacuum drying gets Radix Scutellariae extract.
The preparation method of B, Herba Epimedii extract is: Herba Epimedii is cut into 1cm left and right sides segment, add 10 times of volume 70% alcohol reflux 3 times, each 1 hour, merge extractive liquid, filters, and measuring relative density when filtrate is condensed into 50 ℃ is 1.05～1.15, add 4 times of volume water stirring and dissolving, filter, measuring relative density when filtrate is condensed into 50 ℃ is 1.10～1.20, and vacuum drying gets Herba Epimedii extract.
Get above extract, add 5% low-substituted hydroxypropyl cellulose and 1.5% sorbitol, compacting promptly gets oral cavity disintegration tablet in flakes.
Embodiments of the invention 12: Radix Scutellariae 30g Herba Epimedii 70g
The preparation method of A, Radix Scutellariae extract is: Radix Scutellariae is pulverized, add 8 times of volume 70% alcohol reflux 3 times, each 1 hour, merge extractive liquid,, filter, decompression filtrate recycling ethanol, measuring relative density when being concentrated into 60 ℃ is 1.05～1.15, adds 3 times of volume water dissolutioies, filter, filtrate is crossed the AB-8 macroporous resin column, earlier with 6 times of water and 5 times of 20% alcohol flushing, discards eluent; Reuse 60% alcohol desorption is collected stripping liquid, decompression recycling ethanol, and measuring relative density when being concentrated into 60 ℃ is 1.0～1.05, with hydrochloric acid adjust pH to 2, stirs, and placement is spent the night, and filters, and precipitation washes with water, drains, and vacuum drying gets Radix Scutellariae extract.
B, the preparation method of Herba Epimedii extract is: Herba Epimedii is cut into 1cm left and right sides segment, add 10 times of volume 70% alcohol reflux 3 times, each 1 hour, merge extractive liquid,, filter, measuring relative density when filtrate is condensed into 50 ℃ is 1.05～1.15, adds 3 times of water gagings, stirring and dissolving, filter, filtrate is crossed polyamide column, earlier with 8 times of volume water and 5 times of volume 10% washing with alcohol, discard eluent, 8 times of volume 60% ethanol elutions of reuse are collected eluent, decompression recycling ethanol, measuring relative density when being condensed into 50 ℃ is 1.10～1.20, and vacuum drying gets Herba Epimedii extract.
Get above extract, mixing adds an amount of water for injection dissolving, by volume add 0.3% active carbon, boil, keep little 30min that boils, cold slightly filtration, filtrate add the injection water to ormal weight, with saturated sodium hydroxide solution adjust pH to 6.0～7.0, boil, 5 ℃ of cold preservations are spent the night, and coarse filtration, fine straining are 135 ℃ in inlet temperature, leaving air temp is 60 ℃, and air velocity is 20ms
-1Condition under spray drying get powder, packing promptly gets injectable sterile powder.
Embodiments of the invention 13: Radix Scutellariae extract 50g Herba Epimedii extract 50g
Get the extract of Radix Scutellariae, Herba Epimedii, press medicine and added mannitol than 1: 2.2 with supplementary product consumption, add 1800ml water for injection, stirring makes dissolving, with saturated sodium hydroxide solution adjust pH to 6.0～7.0, add the injection water to 2000ml, mixing, the needle-use activated carbon of adding 0.3%, boiled 30 minutes, coarse filtration, reuse 0.45 μ m and 0.22 μ m microporous filter membrane filter, the filtrate packing, every bottle of 2.0ml, lyophilization, the equilibration time when the balance solidification point of phase I is 0 ℃ is 3 hours, i.e. the time of shelf temperature and product temperature basically identical; The second stage solidification point is from 0 ℃ during to minimum eutectic temperature-18 ℃, and shelf temperature and product temperature equilibration time are 2 hours; Phase III continues to be cooled to-60 ℃, needs 2 hours approximately, keeps this temperature 3 hours, freeze the jail fully until product, promptly begin evacuation, enter drying program, evacuation under-65 ℃ of constant temperature slowly heats up 2～3 ℃/h, to the lowest total of the melting point temperature, the time is about 8 hours, after sublimation drying is finished, continuation is under the low pressure condition, and it is dry to remove residual moisture to heat up, and the time is about 10～12 hours, kept more than 30 ℃ dry 5 hours, gland promptly gets the Injectable sterile block.Wherein, the general flavone content in the Radix Scutellariae extract about 90%; General flavone content about 85% in the Herba Epimedii extract.
Embodiments of the invention 14: Radix Scutellariae extract 50g Herba Epimedii extract 50g
The extract of getting Radix Scutellariae, Herba Epimedii mixes, in adding lactose, mannitol, corn starch, polyvinylpyrrolidone K30, citric acid, sodium citrate, magnesium stearate, xanthein, acetoacetate derivative, sweeting agent, the lemon flavouring one or more, cross 30 mesh sieves, add an amount of Pulvis Talci, micropowder silica gel, evenly mixed, tabletting promptly gets sublingual lozenge.Wherein, the general flavone content in the Radix Scutellariae extract about 65%; The general flavone content about 65% of Herba Epimedii extract.
Embodiments of the invention 15: the quality control method of ejection preparation of the present invention adopts following method:
(1) finger printing of anti-viral pharmaceutical compositions test comprises based on the finger printing of Radix Scutellariae composition characteristics with based on the finger printing of Herba Epimedii composition characteristics;
(2) the differential test method of baicalin, icariin;
(3) baicalin, icariin, content of total flavone method of testing.
Claims (15)
1, a kind of anti virus herb preparation is characterized in that: calculate according to percentage by weight, it is by Radix Scutellariae 1～99%, Herba Epimedii 99～1%, and the extract of perhaps corresponding weight portion medical material adds suitable adjuvant and is made.
2, according to the described anti virus herb preparation of claim 1, it is characterized in that: calculate according to percentage by weight, it is by Radix Scutellariae 20～80%, Herba Epimedii 80～20%, and the extract of perhaps corresponding weight portion medical material adds suitable adjuvant and is made.
3, according to claim 1 or 2 described anti virus herb preparations, it is characterized in that: calculate according to percentage by weight, it is by Radix Scutellariae 40～60%, Herba Epimedii 60～40%, and the extract of perhaps corresponding weight portion medical material adds suitable adjuvant and is made.
4, according to any described anti virus herb preparation of claim 1～3, it is characterized in that: described preparation can be any acceptable dosage form on the pharmaceutics: the injection that is directly used in drug administration by injection, directly supply the venous transfusion of intravenous drip, need to be used for the concentrated solution for injection of intravenous drip and injectable sterile powder and aseptic block and the tablet that makes with freeze-drying or spray drying method after the dilution, capsule, granule, drop pill, pill, soft capsule, oral liquid, all receptible dosage forms on the pharmaceutics such as oral cavity disintegration tablet and dispersible tablet.
5, according to the described anti virus herb preparation of claim 4, it is characterized in that: described preparation can be various injections, drop pill, sublingual lozenge, oral cavity disintegration tablet, dispersible tablet, soft capsule, capsule, ordinary tablet.
6, as any described anti virus herb preparation in the claim 1～5, it is characterized in that: contain flavones ingredient in the described preparation, wherein the flavones ingredient content in the injection is not less than in the preparation 25% of total solid after deduction adjuvant amount and the water quantities.
7, according to the preparation method of any described anti virus herb preparation of claim 1～5, it is characterized in that: get radix scutellariae medicinal materials, add water or ethanol extraction, extracting solution gets the Radix Scutellariae crude extract after carrying out suitably concentrating, and also can further adopt one or more methods mixing uses in alcohol deposition method, acid-base precipitation method, column chromatography, the solvent extraction to make with extra care to such an extent that Radix Scutellariae is made with extra care extract; Get epimedium herb, add water or ethanol extraction, extracting solution gets the Herba Epimedii crude extract after carrying out suitably concentrating, and also can further adopt one or more methods mixing uses in alcohol deposition method, column chromatography, the solvent extraction to make with extra care to such an extent that Herba Epimedii is made with extra care extract; Radix Scutellariae crude extract or refining extract are mixed with Herba Epimedii crude extract or refining extract, make different preparations with diverse ways behind the adding adjuvant.
8, according to claim 4 or 5 described anti virus herb preparations, it is characterized in that: described preparation can make on the basis that in Radix Scutellariae extract, the Herba Epimedii extract one or both is prepared into liposome or pro-liposome.
9, according to the preparation method of the described anti virus herb preparation of claim 7, it is characterized in that:
A, Radix Scutellariae extract are preparations like this: Radix Scutellariae is pulverized, and adds 5-10 times of volume 30-90% alcohol reflux 1-5 time, each 0.1-3 hour, merge extractive liquid, filters decompression filtrate recycling ethanol, concentrate, add 1-8 times of volume water dissolution, filter, filtrate is crossed macroporous resin column, earlier with 3-10 times of water flushing, reuse 30-90% alcohol desorption, collect stripping liquid, decompression recycling ethanol concentrates, and vacuum drying gets Radix Scutellariae extract;
B, Herba Epimedii extract are preparations like this: Herba Epimedii is cut into 0.5-2cm left and right sides segment, adds 5-15 times of volume 30-95% alcohol reflux 1-5 time, each 0.1-3 hour, merge extractive liquid, filters, and filtrate concentrates, add 1-8 times of water gaging, stirring and dissolving filters, filtrate is crossed polyamide column, earlier with 3-10 times of volume water washing, discards eluent, reuse 3-10 times of volume 30-95% ethanol elution collected eluent, decompression recycling ethanol, concentrate, vacuum drying gets Herba Epimedii extract;
Extract is mixed, make different preparations with diverse ways behind the adding adjuvant.
10, according to any described anti virus herb preparation in the claim 1～3,7 or 9, it is characterized in that: adjuvant can be one or more in lactose, galactose, mannitol, corn starch, polyvinylpyrrolidone K30, citric acid, sodium citrate, magnesium stearate, pigment, acetoacetate derivative, sweeting agent, spice, glycine, glucose, sodium chloride, dextran, glycerol, ethanol, propylene glycol, Polyethylene Glycol, sorbitol, tween, HP-, the poloxamer.
11, preparation method according to claim 7 or 9 described anti virus herb preparations, it is characterized in that: the Injectable sterile block in the described preparation prepares like this: get Radix Scutellariae, the extract of Herba Epimedii, press medicine and added mannitol than 1: 2.2 with supplementary product consumption, add 1800ml water for injection, stirring makes dissolving, with saturated sodium hydroxide solution adjust pH to 6.0～7.0, add the injection water to 2000ml, mixing, the needle-use activated carbon of adding 0.3% boiled coarse filtration 30 minutes, reuse 0.45 μ m and 0.22 μ m microporous filter membrane filter, the filtrate packing, every bottle of 2.0ml, lyophilization, equilibration time when the balance solidification point of phase I is 0 ℃ is 3 hours, i.e. the time of shelf temperature and product temperature basically identical; The second stage solidification point is from 0 ℃ during to minimum eutectic temperature-18 ℃, and shelf temperature and product temperature equilibration time are 2 hours; Phase III continues to be cooled to-40 ℃, needs 2 hours approximately, keeps this temperature 3 hours, freeze the jail fully until product, promptly begin evacuation, enter drying program, slowly heat up, 2～3 ℃/h, to the lowest total of the melting point temperature, time is about 8 hours, after sublimation drying is finished, continues under the low pressure condition, heating up, dry the time is about 10～12 hours to remove residual moisture, keeps more than 30 ℃ dry 5 hours, gland promptly gets the Injectable sterile block.
12, according to the preparation method of claim 7 or 9 any described anti virus herb preparations, it is characterized in that: described sublingual lozenge is such preparation: the extract of getting Radix Scutellariae, Herba Epimedii mixes, add 15% lactose, 20% corn starch, 15% polyvinylpyrrolidone K30, cross 30 mesh sieves, add an amount of Pulvis Talci, micropowder silica gel, evenly mixed, tabletting promptly gets sublingual lozenge.
13, according to the preparation method of the described anti virus herb preparation of claim 8, it is characterized in that: the pro-liposome in the described preparation prepares like this: get in Radix Scutellariae extract, the Herba Epimedii extract one or both, add the phosphate buffer dissolving, add in the fused solution of fabaceous lecithin, cholesterol and 18-amine. mixing, stir supersound process, freezing, the dry powder that obtains sieves, promptly.
14, as the quality control method of any described anti virus herb preparation in the claim 1～13, it is characterized in that: described method of quality control comprise in the following method partly or entirely:
(1) finger printing of anti-viral pharmaceutical compositions test comprises based on the finger printing of Radix Scutellariae composition characteristics with based in the finger printing of Herba Epimedii composition characteristics one or more;
(2) radix scutellariae medicinal materials, epimedium herb, baicalin, noroxylin, chrysin, icariin, icariside I, Herba Epimedii glycosides A, B, C, precious icariin I, II, the content test method of all or part of composition in VI, the Quercetin;
(3) baicalin, noroxylin, chrysin, icariin, icariside I, Herba Epimedii glycosides A, B, C, precious icariin I, II, the content test method of all or part of composition in VI, Quercetin, the total flavones;
15, as the application of any described anti virus herb preparation in the claim 1～3, it is characterized in that: described preparation is used to prepare the medicine of diseases such as treatment pneumonia, respiratory tract infection, influenza, bird flu, SARS, asthma, human body immunity improving power.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20190300560A1 (en) * | 2015-05-20 | 2019-10-03 | Foshan Golden Health Technology Co., Ltd. | Icariside compound, preparation method thereof, and application thereof |
| CN111759880A (en) * | 2020-06-09 | 2020-10-13 | 广东金骏康生物技术有限公司 | Epimedium herb extract and application thereof in preventing or treating coronavirus |
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2005
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20190300560A1 (en) * | 2015-05-20 | 2019-10-03 | Foshan Golden Health Technology Co., Ltd. | Icariside compound, preparation method thereof, and application thereof |
| US11117919B2 (en) * | 2015-05-20 | 2021-09-14 | Golden Health (Guangdong) Biotechnology Co. Ltd. | Icariside compound, preparation method thereof, and application thereof |
| CN111759880A (en) * | 2020-06-09 | 2020-10-13 | 广东金骏康生物技术有限公司 | Epimedium herb extract and application thereof in preventing or treating coronavirus |
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