CN1967243A - Method for rapid identifying Chinese traditional medicine granule - Google Patents
Method for rapid identifying Chinese traditional medicine granule Download PDFInfo
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- CN1967243A CN1967243A CN 200610123303 CN200610123303A CN1967243A CN 1967243 A CN1967243 A CN 1967243A CN 200610123303 CN200610123303 CN 200610123303 CN 200610123303 A CN200610123303 A CN 200610123303A CN 1967243 A CN1967243 A CN 1967243A
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- granule
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- chinese medicinal
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Abstract
The invention is a pharmaceutical testing technology, and it discloses a fast identification Chinese medicinal granules method. The method is to identify the Chinese medicinal granules using solvent extraction, and then adopt UV spectra identification to obtain the characteristics map, including maximum absorption wavelength. Comparing the map with the known contradistinguish map, if the maximum absorption wavelength displacement not exceeding +-2nm, the corresponding peak shape is consistent, which can determine the formula granules species being the corresponding species in the contradistinguish map. The method is simple, rapid, accurate, and greatly improves the test efficiency, lowers the testing cost.
Description
Technical field:
The present invention relates to the drug inspection field, refer in particular to a kind of method of quick discriminating Chinese medicinal granule.
Background technology:
Chinese medicine is in the existing long history of the application of China, even in today of scientific and technical high speed development, traditional Chinese medicine is also enjoyed more and more higher reputation in the world, but, the application method of traditional decoction " medicine materical crude slice is used as medicine, and faces with frying in shallow oil soup " shows and " inadaptability " of the modern life and " uncertainty " of clinical efficacy day by day.Directly be used as medicine with root, stem, leaf, flower, fruit, the method of Jian Zhuing voluntarily, can not satisfy the needs of the people far away to the selection and the traditional Chinese medicine development of traditional Chinese medicine medical services, also have a strong impact on simultaneously and restricted tcm clinical practice subject modernization development, hindered the raising of this traditional national industry of the prepared slices of Chinese crude drugs self society, economic benefit and traditional Chinese medicine modernization, industrialization, internationalization process.In view of this, Guangdong Yifang Pharmaceutical Co., Ltd has begun to carry out the research of " research and development of Chinese medicinal granule " projects from 1993, be intended to adopt the advanced technologies technology of modern pharmaceutical industry, with the prepared slices of Chinese crude drugs be prepared into separately can instant-drink Chinese medicinal granule, and replenish and development as a kind of of prepared slices of Chinese crude drugs decoction with this.
Chinese medicinal granule with its science, flexibly, health, good effect, advantage such as take, easy to carry, obtain application more and more widely, presented good industrialization, the international development impetus and prospect.Since Chinese crude drug (medicine materical crude slice) through extract, concentrate, dry, make granule after, the feature that has not possessed medicinal material, can't differentiate accurately Chinese medicinal granule only according to appearance character, must learn detection method analysis by physics and chemistry, still, because the kind of granule reaches more than 700, adopt discrimination methods such as thin-layer chromatography, its workload is big, and is time-consuming, and it is higher to detect cost.The present inventor has found a kind of method of quick discriminating Chinese medicinal granule through years of researches, accumulation and summary, and this method is simple, quick, accurate, has improved checkability widely, has reduced inspection cost.
Summary of the invention:
The invention provides a kind of method of quick discriminating Chinese medicinal granule, purpose is to improve simply, fast and accurately the checkability of granule.
The object of the present invention is achieved like this: a kind of method of quick discriminating Chinese medicinal granule, and the concrete steps of this method are:
Chinese medicinal granule to be identified is extracted with solvent, adopt the ultraviolet spectrum differential method to obtain to comprise the characteristic collection of illustrative plates of maximum absorption wavelength again; This collection of illustrative plates and known contrast collection of illustrative plates compare, if the maximum absorption wavelength displacement is no more than ± 2nm, corresponding peak shape unanimity can determine that this granule kind is the corresponding kind of contrast collection of illustrative plates.
Described solvent comprises: organic solvents such as water, methyl alcohol, ethanol, absolute ethyl alcohol, ethyl acetate, acetone, chloroform, ether, sherwood oil, the wherein potpourri of several solvents.
Described contrast collection of illustrative plates is the medicinal material that adopts through identifying, the contrast collection of illustrative plates that the extracting method of the same granule of being differentiated is made.
Method of the present invention compared with prior art has following advantage: 1. easy and simple to handle, quick, the Chinese medicinal granule that need are differentiated extracts with solvent supersonic, can carry out ultraviolet spectrum after suitably handling and differentiate, than other discrimination method, saves time, saves trouble; 2. assay is accurate, has carried out the discriminating of nearly 500 batches of granules, rate of accuracy reached to 100%; 3. be applicable to market surpervision, after Chinese medicinal granule is put on market, how differentiate immediately, the invention provides a kind of effective method for quick identification; 4. inspection cost is low.
Description of drawings:
Fig. 1 is that the ultraviolet spectrum of the golden cypress granule among the embodiment 1 is differentiated collection of illustrative plates
Fig. 2 is that the Radix-paeoniae-rubra formula granules ultraviolet spectrum among the embodiment 2 is differentiated figure
Fig. 3 is that the tangerine peel dispensing granule ultraviolet spectrum among the embodiment 3 is differentiated figure
Description of reference numerals: the label 1 among Fig. 1 is that golden cypress reference substance, 2 is golden cypress granule test sample; Label 1 among Fig. 2 is that radix paeoniae rubrathe reference substance, 2 is the Radix-paeoniae-rubra formula granules test sample; Label 1 among Fig. 3 is that dried orange peel reference substance, 2 is the tangerine peel dispensing granule test sample.
Embodiment:
Embodiment 1
The discriminating of golden cypress granule
Take by weighing the about 0.5g of golden cypress control medicinal material powder, put in the tool plug conical flask, add water 10ml, ultrasonic Extraction 5 minutes, soaking at room temperature 30 minutes filters.Get filtrate 0.5ml and put in the 25ml colorimetric test tube, add water and be settled to scale, shake up, as the golden cypress reference substance solution.
Take by weighing the about 0.5g of golden cypress granule powder, put in the tool plug conical flask, add water 10ml, ultrasonic Extraction 5 minutes, soaking at room temperature 30 minutes filters.Get filtrate 0.2ml and put in the 25ml colorimetric test tube, add water and be settled to scale, shake up, as golden cypress granule need testing solution.
Getting above-mentioned two kinds of solution, is blank with water, measure according to UV-VIS spectrophotometry (appendix VA of Chinese Pharmacopoeia version in 2005), 276nm (± 2nm) (there is absorption maximum at ± 2nm) wavelength place, and data and collection of illustrative plates are seen Fig. 1, table 1 with 332nm.
The absorption maximum and the absorbance measurement result of table 1 golden cypress granule ultraviolet spectrum
| The name of an article | λ max(water) [A max] | |
| The golden cypress reference substance | 332nm[1.6] | 276nm[1.7] |
| Golden cypress granule test sample | 331nm[2.3] | 277nm[2.4] |
Embodiment 2
The discriminating of Radix-paeoniae-rubra formula granules
Take by weighing the about 0.5g of radix paeoniae rubrathe control medicinal material powder, add water 50ml, boiled 30 minutes, filter the filtrate evaporate to dryness, residue adds methyl alcohol 10ml, soaking at room temperature 30 minutes, and sonicated 5 minutes filters, get filtrate 0.2ml and put in the 25ml colorimetric test tube, add methyl alcohol, shake up, as radix paeoniae rubrathe reference substance solution to scale.
Take by weighing the about 0.5g of Radix-paeoniae-rubra formula granules powder, add methyl alcohol 10ml, soaking at room temperature 30 minutes, sonicated 5 minutes filters, and gets filtrate 0.2ml and puts in the 25ml colorimetric test tube, adds methyl alcohol to scale, shakes up, as the Radix-paeoniae-rubra formula granules need testing solution.
Getting above-mentioned two kinds of solution, is blank with methyl alcohol, measure according to UV-VIS spectrophotometry (appendix VA of Chinese Pharmacopoeia version in 2005), 274nm (± 2nm) there is absorption maximum at the wavelength place, data and collection of illustrative plates are seen Fig. 2, table 2.
Table 2 Radix-paeoniae-rubra formula granules UV spectrum authentication data
| The name of an article | Maximum absorption band place value (nm) |
| Radix paeoniae rubrathe reference substance Radix-paeoniae-rubra formula granules test sample | 275 274 |
Embodiment 3
The discriminating of tangerine peel dispensing granule
Take by weighing the about 0.5g of dried orange peel control medicinal material powder, add water 50ml, boiled 30 minutes, filter, filtrate evaporate to dryness, residue add absolute ethyl alcohol 10ml, soaking at room temperature 30 minutes, sonicated 5 minutes, filter, get filtrate 0.2ml and put in the 25ml colorimetric test tube, add absolute ethyl alcohol to scale, shake up, as the dried orange peel reference substance solution.
Take by weighing the about 0.5g of tangerine peel dispensing granule powder, add absolute ethyl alcohol l0ml, soaking at room temperature 30 minutes, sonicated 5 minutes filters, and gets filtrate 0.2ml and puts in the 25ml colorimetric test tube, adds absolute ethyl alcohol to scale, shakes up, as the tangerine peel dispensing granule need testing solution.
Getting above-mentioned two kinds of solution, is blank with the absolute ethyl alcohol, measures according to UV-VIS spectrophotometry (appendix VA of Chinese Pharmacopoeia version in 2005), and there is absorption maximum at the place at the 279nm wavelength, and data and collection of illustrative plates are seen Fig. 3, table 3.
Table 3 tangerine peel dispensing granule UV spectrum authentication data
| The name of an article | Maximum absorption band place value (nm) |
| Dried orange peel reference substance tangerine peel dispensing granule test sample | 279 279 |
Claims (3)
1. a method of differentiating Chinese medicinal granule fast is characterized in that: Chinese medicinal granule to be identified is extracted with solvent, adopt the ultraviolet spectrum differential method to obtain to comprise the characteristic collection of illustrative plates of maximum absorption wavelength again; This collection of illustrative plates and known contrast collection of illustrative plates compare, if the maximum absorption wavelength displacement is no more than ± 2nm, corresponding peak shape unanimity can determine that this granule kind is the corresponding kind of contrast collection of illustrative plates.
2. the method for a kind of quick discriminating Chinese medicinal granule according to claim 1 is characterized in that: described solvent comprises a kind of solvent or the potpourri of several organic solvents wherein in water, methyl alcohol, ethanol, absolute ethyl alcohol, ethyl acetate, acetone, chloroform, ether, the sherwood oil.
3. the method for a kind of quick discriminating Chinese medicinal granule according to claim 1 is characterized in that: described contrast collection of illustrative plates is the medicinal material that adopts through identifying, the contrast collection of illustrative plates that the extracting method of the same granule of being differentiated is made.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610123303 CN1967243A (en) | 2006-11-03 | 2006-11-03 | Method for rapid identifying Chinese traditional medicine granule |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610123303 CN1967243A (en) | 2006-11-03 | 2006-11-03 | Method for rapid identifying Chinese traditional medicine granule |
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| CN1967243A true CN1967243A (en) | 2007-05-23 |
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| CN 200610123303 Pending CN1967243A (en) | 2006-11-03 | 2006-11-03 | Method for rapid identifying Chinese traditional medicine granule |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102288551A (en) * | 2011-04-01 | 2011-12-21 | 尚振刚 | Method for distinguishing traditional Chinese medicinal material by utilizing hyperspectral data |
| CN106918568A (en) * | 2017-03-28 | 2017-07-04 | 福建中医药大学 | A kind of method for differentiating Radix Angelicae Sinensis, lovage and levisticum |
-
2006
- 2006-11-03 CN CN 200610123303 patent/CN1967243A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102288551A (en) * | 2011-04-01 | 2011-12-21 | 尚振刚 | Method for distinguishing traditional Chinese medicinal material by utilizing hyperspectral data |
| CN106918568A (en) * | 2017-03-28 | 2017-07-04 | 福建中医药大学 | A kind of method for differentiating Radix Angelicae Sinensis, lovage and levisticum |
| CN106918568B (en) * | 2017-03-28 | 2020-04-28 | 福建中医药大学 | Method for identifying angelica, levisticum and radix angelicae pubescentis |
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