CN1958070A - Triple high immunity immunoglobulin for anti transmissible gastroenteritis of pig, epidemic diarrhea of pig, and rotavirus of pig - Google Patents
Triple high immunity immunoglobulin for anti transmissible gastroenteritis of pig, epidemic diarrhea of pig, and rotavirus of pig Download PDFInfo
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Abstract
An immunoglobulin for preventing the infectious gastroenteritis, epidemic diarrhea and rotavirus of hog is prepared from their immunogens through cell culture, concentrating and proportionally mixing it with complete Frennd's adjuvant. It features that the glycoside-peptide injection is used as its immunopotentiator.
Description
Technical field
The invention belongs to biological technical field, be specifically related to the immunoglobulin of a kind of anti-swine infectious enterogastritis, porcine epizootic diarrhea, porcine rotavirus, international Patent classificating number is: A61K39/395.
Background technology
Transmissible gastroenteritis of swine, porcine epizootic diarrhea and porcine rotavirus infect be verified at present, cause that three important viral infectious diseases of diarrheal take place pig.Mainly the influence to piglet is bigger for these three kinds of diseases, is the main diseases diarrhea virus that causes piglet death.These three kinds of usually mixed infections of disease; all closely similar in epidemiology, clinical symptoms, pathological anatomy variation; very difficult difference is any viral infection on earth; and morbidity rapidly; nearly several hrs after morbidity; just can involve full group, mainly be the large quantities of death that cause nursery-age pig, causes tremendous loss for the production of raising pigs.
Transmissible gastroenteritis of swine (Porcine Transmissible Gastroenteritis, TGE) be transmissible gastro-enteritis virus (Porcine TransmissibleGastroenteritis virus by coronaviridae (Coronaviridae), coronavirus genus (Coronavirus), what TGEV) cause is the infectious disease of feature with pig vomiting, diarrhoea, dehydration, newborn piglet is had the height fatality rate, be generally 100%.Though the pig of all ages and classes is to the equal susceptible of this virus, after the above pig of 5 all tinkling of pieces of jade infected, mortality rate was very low.Doyle in 1945 in U.S.'s reported first after this disease, many in the world countries have all reported transmissible gastroenteritis of swine in succession.China from 1956 after this disease takes place in Guangdong first, mostly province generation that transmissible gastroenteritis of swine is also arranged of the whole nation has brought serious harm to pig industry.
Porcine epizootic diarrhea (Porcine Epidemic Diarrhae, PED) be Porcine epidemic diarrhea virus (Porcine Epidemic Diarrhae virus, a kind of Intestinum Sus domestica road transmission disease that PEDV) causes by coronaviridae (Coronaviridae), coronavirus genus (Coronavirus).With watery diarrhea, vomiting and dehydration is feature.The equal susceptible of the pig at various ages, the sickness rate of suckling pig, feeder pig or growing and fattening pigs can reach 100%, and especially suckling pig is injured the most serious.Porcine epizootic diarrhea mainly occurs in winter and rasputitsa comparatively cold season, but also can take place summer.Expect the generation that multinational family has reported porcine epizootic diarrhea in succession the beginning of the seventies in last century, China begins that from the eighties in last century the report that porcine epizootic diarrhea takes place is arranged successively.
Porcine rotavirus infects a kind of acute infectious disease of the digestive tract that (Porcine Rotavirus Infection) is a kind of children pig in age of causing of the rotavirus by the rotavirus (Rotavirus) of Reoviridae (Reoviridae).The cardinal symptom of children pig is anorexia, uneasiness, can occur vomiting once in a while.The serious symptom pig high capacity diarrhoea occurs in morbidity after 1～4 hour, draining sample feces, and color is yellow to white, contains floccule.Suffered from diarrhoea sustainable 3～5 days, during suffering from diarrhoea, dehydration and dead appears in piglet, and mortality rate can reach 50%～100%.Epidemiological study shows that the band of rotavirus poison rate is very high in the swinery, and middle pig and big pig mostly are subclinical infection and inapparent infection, promptly only carries virus but does not fall ill the just piglet of morbidity.Therefore say that rotavirus infection just influences a kind of infectious disease of piglet survival ratio.
At present mainly prevent the generation of these three kinds of diseases with vaccine.Because also not at the specific medicament of viral disease, therefore on clinical treatment, mainly take symptomatic treatment and with the generation of antibiotics complication prevention, its therapeutic effect is undesirable.In addition, because these three kinds of disease incidences rapidly, after occurring suffering from diarrhoea, piglet dewaters owing to diarrhoea mostly, will soon die owing to dying of exhaustion.Therefore be badly in need of a kind of specificity that both had aborning, curative effect is certain again, the treatment preparation that therapeutic effect manifests rapidly.And anti-swine infectious enterogastritis, porcine epizootic diarrhea, porcine rotavirus triad high immune immunoglobulin are exactly the medicine with above-mentioned advantage.
Summary of the invention
Goal of the invention of the present invention provides three kinds of immunogen preparing treatments of a kind of employing or prevents transmissible gastroenteritis of swine, porcine epizootic diarrhea, three kinds of diseases of porcine rotavirus, has the specificity height, with strong points, anti-swine infectious enterogastritis, porcine epizootic diarrhea, porcine rotavirus triad high immune immunoglobulin that curative effect is certain.
Immunogen of the present invention adopts cell culture to add complete Freund adjuvant after concentrating and is prepared from, and wherein transmissible gastro-enteritis virus is cultivated with piglets kidney primary cell or porcine kidney cell (PK-15) passage cell; Porcine epidemic diarrhea virus is cultivated with the Vero cell; Porcine rotavirus MA
104Cell line or CV
1Cell culture; The school valency that concentrates these three kinds of viruses of back must not be less than 10
6, with these three kinds of immunogens with volume ratio (1～3): (1～3): add Freund's complete adjuvant at 1: 1 as the immunogen immune animal with volume ratio after the mixed of (1～3); (denomination of invention is the Heterosides peptide injection that uses the Jilin whale to resemble animal pharmaceutcal corporation, Ltd simultaneously to be developed into: a kind of Heterosides peptide injection that improves the animal immune function, Chinese patent application number is: 2,005 10016626.2) as immunostimulant, 5 milliliters/, to improve antibody titer.
Immune animal of the present invention is used through the pig of strict quarantine, horse (mule, donkey) as immune animal;
Immune programme for children
Immunization route adopts Intradermal, subcutaneous or acupuncture point, muscle multi-point injection.Fundamental immunity dosage is every pig 3ml, horse (mule, donkey) 5ml.The immunization ways pig is subcutaneous or the muscle multi-point injection, and horse (mule, donkey) is used Intradermal, subcutaneous or acupuncture point multi-point injection.Carried out the booster immunization first time in 28 days at interval, immunizing dose is pig 8ml, horse (mule, donkey) 12ml.Booster immunization was carried out once in 15 days in later every interval, behind 2～4 booster immunizations, detect antibody titer, it is qualified that antibody titer 〉=2048 o'clock are considered as, do not reach this standard as antibody titer, continue booster immunization once, then to the blood sampling of horse (mule, donkey) jugular vein or to immune swine carotid artery or the direct blood-letting of heart.
Antibody titer detects
Use the ELISA method that detects these three kinds of diseases to detect serum antibody titer respectively.Detect antigen and use cell culture through virus spissated and the process respective handling, second antibody adopts anti-pig of corresponding rabbit (mouse-anti pig) or the anti-horse of rabbit (mouse-anti horse), sets up the contrast of positive and negative serum simultaneously.The serum antibody titer eligible can be taken a blood sample or dispatch from the factory as qualified products.
Blood sampling and serum extract
At first drench fully with the container inner wall of 1%～5% aseptic sodium chloride solution with blood sampling.Each container should be indicated animal number, last booster immunization date and blood sampling date.Behind the sterile blood sampling with the blood gathered 20 ℃～25 ℃ of placements, treat that it all solidifies and when having serum to separate out, adds the ram of sterilization, after 48～72 hours, extracts serum with sterile manner.Press 0.5% of serum total amount simultaneously and add chloroform, 0.0035% adding thimerosal, the sealing of jumping a queue fully shakes up, and is positioned over 2 ℃～15 ℃ dark cold places, the precipitation clarification.Simultaneously every bottle of serum should post animal number, serum amount and separate the date.
Blood plasma extracts
In the sterilization vial that fills 5% sterilization liquor sodii citratis, the ratio of blood and liquor sodii citratis is 9: 1 with blood collecting; Through 48～72 hours precipitations, to extract supernatant, and add chloroform, 0.0035% adding thimerosal by 0.5% of total amount, the sealing of jumping a queue fully shakes up.Every bottle of blood plasma should be made itemized record.
Serum (blood plasma) is refining
Will be mixed in the same sterilization container by purified serum (blood plasma), 2 times of amounts of pressing serum (blood plasma) total amount add distilled water, stir; Regulate pH to 3.0 with 2mol/L hydrochloric acid, when adding hydrochloric acid adjusting pH, slowly add, the limit edged stirs the limit and measures, and prevents that the local pH peracid from exerting an influence to the activity of immunoglobulin.Placed 60 minutes, the sodium hydroxide solution of reuse 2mol/L is regulated pH to 3.2～3.4.Amount according to every 1ml 6～9 units adds pepsin then, and adds toluene by 0.2% of total amount, digests 2～24 hours under 29 ℃～31 ℃ conditions, will often stir between the period of digestion.
The solution that has digested is added ammonium sulfate according to 15% of total amount, fully after the dissolving, regulate pH to 4.5～4.7,60 ℃ water-bath 60 minutes with the sodium hydroxide solution of 2mol/L.When treating that solution is cooled to below 43 ℃, filter, collect filtrate, remove precipitation with canvas.The filtrate of collecting is measured, and be put in the container of same sterilization, regulate pH to 7.1～7.3, add ammonium sulfate, after stirring, filter the collecting precipitation thing with the canvas of sterilizing according to 20% of total amount with the sodium hydroxide solution of 2mol/L.Then precipitation is carried out weighing, puts in the sterile chamber, and in precipitate, add 5～6 times distilled water, the dissolution precipitation thing, treat that precipitate fully dissolves after, add 10% alum solution according to total amount, make the Alumen total content reach 1%.Reuse 2mol/L sodium hydroxide solution is regulated pH to 7.7～7.8, leaves standstill and makes its precipitation in 2～4 hours, filters with aseptic canvas then, collects filtrate.
The filtrate of collecting is positioned in the same sterile chamber, add ammonium sulfate by 38% of total amount, fully stirring the back filters with aseptic canvas, abandon the supernatant collecting precipitation, precipitation is pressed dry back bag filter bag distribution packaging, every bag of about 200～250g adds a little chloroform, tightens to be positioned in the gutter channel dialysis behind the bag filter 48～72 hours.Serum in the collecting bag adds chloroform, 0.85% by 0.5% of total amount and adds sodium chloride then, and 0.0035% adds thimerosal.Fully the 0.22um membrane filtration is used in the dissolving back under aseptic condition, is positioned over 5 ℃～15 ℃ and staticly settles.The serum that takes a morsel then carries out antibody titer and detects, and its protection effectiveness is measured.
According to " People's Republic of China's veterinary biologics rules " that The Ministry of Agriculture of the People's Republic of China, MOA published in 2000 the serum of producing is carried out steriling test.
Protection is renderd a service and is detected test
With 60 body weight 2～3kg, 3～5 days the suckling pig in birth back is divided into 8 groups at random, preceding four groups is 10 every group of test group, with what cross after testing, virus titer is 10 to first, second and third group respectively
6Transmissible gastroenteritis of swine, porcine epizootic diarrhea, porcine rotavirus liquid 2ml oral infection test piglet, same use detected through ELISA, and the high immune immunoglobulin 2～3ml of antibody titer 〉=2048 gives the test pig intramuscular injection; Give test pig oral with three kinds of viral mixed liquors for the 4th group, injection of antibodies is tired 〉=high immune immunoglobulin 2～4ml of 2048 simultaneously; Observed 20 continuously, diarrhoea person occurs and be considered as morbidity, diarrhoea person do not occur and be considered as protection.Five, six, seven, eight groups is matched group, every group of 5 pigs, and the 5th, six, seven group is 10 with virus titer respectively
6Transmissible gastroenteritis of swine, porcine epizootic diarrhea, porcine rotavirus liquid 2ml oral infection, the 8th group is infected with three kinds of viral mixed liquors, substitutes high immune immunoglobulin with normal saline, and observing time is identical with test group with criterion.Duration of test is to the dead test pig of falling ill, and the piglet to matched group carries out pathological anatomy after off-test, observes the pathological change that whether takes place with disease association.
Testing result is renderd a service in protection
First group of high immune immunoglobulin test group with transmissible gastro-enteritis virus all protected, and do not find the generation of diarrhoea phenomenon.Corresponding matched group is suffered from diarrhoea for the 5th group 5/5.The do not suffer from diarrhoea generation of phenomenon of second group of test pig 9/10 with the Porcine epidemic diarrhea virus counteracting toxic substances, its spirit, suckling are all normal, wherein have a pig slight symptom of diarrhea to occur, and untreated is considered as protection spontaneous remission in second day.The 6th group matched group is all fallen ill.All protect with porcine rotavirus counteracting toxic substances group for the 3rd group, its matched group (the 7th group) is all suffered from diarrhoea.The 4th group of test group 9/10 protection with hybrid virus liquid counteracting toxic substances, 1/10 diarrhoea occurs, and untreated was spontaneous remission in the 3rd day.Its matched group (the 8th group) is morbidity all.
The product packing
After suitably diluting, during as the finished product packing, antibody titer should exceed 20% than required standard with semi-finished product, even the antibody titer of time spent is 〉=2048 o'clock, the antibody titer of packing finished product answers 〉=2640.Using dosage is the 1ml/kg body weight.Storage life is 36 months.
The present invention has the following advantages and good effect:
1. safety uses pepsin that immunoglobulin is digested in the processing technique of immunoglobulin fortunately, the FC segment of antibody is cut away, make the molecular weight of immunoglobulin dwindle one times nearly, with the ammonium sulphate precipitation method albumin of blood weight is removed simultaneously, so just can not caused allergic reaction;
2. because this antibody has the specificity of height, therefore, with this species specificity high immune immunoglobulin the morbidity pig is treated or promptly prevents and to obtain rapid, special curative effect;
3. the good practical application of clinical application effect proves, with the treatment of this high immune immunoglobulin or prevention transmissible gastroenteritis of swine, porcine epizootic diarrhea, its curative effect of porcine rotavirus diarrhoea is really and rapidly, after the medication (intramuscular injection) can see obvious effects, in conjunction with symptomatic therapy such as fluid infusion, antiacid poisonings, can reach clinical cure at 24 hours, the clinical Signs of item such as ill pig feed desire, spirit all recover normal.Generally comparatively light-duty case, a drug just can be cured, and for indivedual severe cases, duplicate injection once can reach the effect of clinical cure again after 1～2 day.
5. can not produce drug residue because the half-life of immunoglobulin is short, metabolism is very fast, does not have cumulative action in vivo, does not have drug residue.Can produce the pork product of high-quality, green.
7. preservation and this product easy to use are light yellow transparent liquid, preserve for 4 ℃ and detect antibody titer in 36 months still more than 〉=2048, and be highly stable.In the use, a pin can prevent and treat three kinds of diseases.
The specific embodiment
Embodiment 1:
Immunogen of the present invention adopts cell culture to add complete Freund adjuvant after concentrating and is prepared from, and wherein transmissible gastro-enteritis virus is cultivated with piglets kidney primary cell or porcine kidney cell (PK-15) passage cell; Porcine epidemic diarrhea virus is cultivated with the Vero cell; Porcine rotavirus MA
104Cell line or CV
1Cell culture; The school valency that concentrates these three kinds of viruses of back must not be less than 10
6, these three kinds of immunogens are added Freund's complete adjuvant as the immunogen immune animal with volume ratio 1: 1 after with 1: 2: 1 mixed; Use the Jilin whale to resemble the Heterosides peptide injection that animal pharmaceutcal corporation, Ltd is developed into simultaneously, 5 milliliters/, as immunostimulant.
Immune programme for children
Immunization route adopts Intradermal, subcutaneous or acupuncture point, muscle multi-point injection.Fundamental immunity dosage is every pig 3ml, horse (mule, donkey) 5ml.The immunization ways pig is subcutaneous or the muscle multi-point injection, and horse (mule, donkey) is used Intradermal, subcutaneous or acupuncture point multi-point injection.Carried out the booster immunization first time in 28 days at interval, immunizing dose is pig 8ml, horse (mule, donkey) 12ml.Booster immunization was carried out once in 15 days in later every interval, behind 2～4 booster immunizations, detect antibody titer, it is qualified that antibody titer 〉=2048 o'clock are considered as, do not reach this standard as antibody titer, continue booster immunization once, then to the blood sampling of horse (mule, donkey) jugular vein or to immune swine carotid artery or the direct blood-letting of heart.
Antibody titer detects
Use the ELISA method that detects these three kinds of diseases to detect serum antibody titer respectively.Detect antigen and use cell culture through virus spissated and the process respective handling, second antibody adopts anti-pig of corresponding rabbit (mouse-anti pig) or the anti-horse of rabbit (mouse-anti horse), sets up the contrast of positive and negative serum simultaneously.The serum antibody titer eligible can be taken a blood sample or dispatch from the factory as qualified products.
Blood sampling and serum extract
Behind the sterile blood sampling with the blood gathered at 20 ℃～25 ℃ of placements.Serum extracts: treat that it all solidifies and when having serum to separate out, adds the ram of sterilization, after 48～72 hours, extracts serum with sterile manner.Press 0.5% of serum total amount simultaneously and add chloroform, 0.0035% adding thimerosal, the sealing of jumping a queue fully shakes up, and is positioned over 2 ℃～15 ℃ dark cold places, the precipitation clarification.
Blood plasma extracts
In the sterilization vial that fills 5% sterilization liquor sodii citratis, the ratio of blood and liquor sodii citratis is 9: 1 with blood collecting; Through 48～72 hours precipitations, extract supernatant.
Serum (blood plasma) is refining
Will be mixed in the same sterilization container by purified serum (blood plasma), by 2 times of amount adding distilled water of total amount, regulate pH to 3.0 with 2mol/L hydrochloric acid, placed 60 minutes, the sodium hydroxide solution of reuse 2mol/L is regulated pH to 3.2～3.4.Amount according to every 1ml 6～9 units adds pepsin then, digests 2～24 hours under 29 ℃～31 ℃ conditions.
The solution that has digested is added ammonium sulfate according to 15% of total amount, regulate pH to 4.5～4.7,60 ℃ water-bath 60 minutes with the sodium hydroxide solution of 2mol/L.When treating that solution is cooled to below 43 ℃, filter, collect filtrate, remove precipitation.Metering also is put in the same container, regulates pH to 7.1～7.3 with the sodium hydroxide solution of 2mol/L, adds ammonium sulfate according to 20% of total amount, uses the canvas of sterilization to filter the collecting precipitation thing.Put into after the weighing in the container, add 5～6 times distilled water, behind the dissolution precipitation thing, according to the alum solution of total amount adding 10%, reuse 2mol/L sodium hydroxide solution is regulated pH to 7.7～7.8, leaves standstill and makes its precipitation in 2～4 hours, filters and also collects filtrate.
The filtrate of collecting is positioned in the same container, adds ammonium sulfate, stir the back and filter with aseptic canvas by 38% of total amount, abandon the supernatant collecting precipitation, precipitation is pressed dry the back use the bag filter bag distribution packaging, every bag of about 200～250g tightens to be positioned over behind the bag filter in the gutter channel and dialysed 48～72 hours.Serum in the collecting bag, fully the 0.22um membrane filtration is used in the dissolving back under aseptic condition, is positioned over 5 ℃～15 ℃ and staticly settles.
According to " People's Republic of China's veterinary biologics rules " that The Ministry of Agriculture of the People's Republic of China, MOA published in 2000 the serum of producing is carried out steriling test.
The product packing
After suitably diluting, during as the finished product packing, antibody titer should exceed 20% than required standard with semi-finished product, even the antibody titer of time spent is 〉=2048 o'clock, the antibody titer of packing finished product answers 〉=2640.Using dosage is the 1ml/kg body weight.Storage life is 36 months.
Embodiment 2:
Immunogen of the present invention adopts cell culture to add complete Freund adjuvant after concentrating and is prepared from, and wherein transmissible gastro-enteritis virus is cultivated with piglets kidney primary cell or porcine kidney cell (PK-15) passage cell; Porcine epidemic diarrhea virus is cultivated with the Vero cell; Porcine rotavirus MA
104Cell line or CV
1Cell culture; The school valency that concentrates these three kinds of viruses of back must not be less than 10
6, these three kinds of immunogens are added Freund adjuvant as the immunogen immune animal after with 1.5: 2: 3 mixed; Use the Jilin whale to resemble Heterosides peptide injection that animal pharmaceutcal corporation, Ltd is developed into simultaneously as immunostimulant.
Preparation method is identical with embodiment 1.
Claims (3)
1, anti-swine infectious enterogastritis, porcine epizootic diarrhea, porcine rotavirus triad high immune immunoglobulin,
Immunogen adopt transmissible gastro-enteritis virus with piglets kidney primary cell or the cultivation of porcine kidney cell (PK-15) passage cell, Porcine epidemic diarrhea virus with the Vero cell cultivate, porcine rotavirus MA
104Cell line or CV
1Cell culture;
Immunological adjuvant is that Fu Shi helps complete agent;
Immunostimulant resembles the Heterosides peptide injection of animal pharmaceutcal corporation, Ltd development for the Jilin whale.
2, anti-swine infectious enterogastritis according to claim 1, porcine epizootic diarrhea, porcine rotavirus triad high immune immunoglobulin is characterized in that:
Described transmissible gastro-enteritis virus, Porcine epidemic diarrhea virus and three kinds of immunogens of porcine rotavirus are with (1～3): (1～3): the mixed of (1～3);
Described Freund's complete adjuvant and immunogenic volume mixture ratio are 1: 1;
Described immunostimulant Heterosides peptide injection is 5 milliliters/.
3, anti-swine infectious enterogastritis according to claim 1, porcine epizootic diarrhea, porcine rotavirus triad high immune immunoglobulin is characterized in that:
Described transmissible gastro-enteritis virus, Porcine epidemic diarrhea virus and three kinds of immunogens of porcine rotavirus concentrate must not tire of back virus and are less than 10
6
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| CN101948536A (en) * | 2010-05-10 | 2011-01-19 | 洛阳普莱柯生物工程有限公司 | Porcine transmissible gastroenteritis virus (PTGEV) and porcine epidemic diarrhea virus (PEDV) dual yolk antibody and preparation method thereof |
| CN102949718A (en) * | 2011-08-26 | 2013-03-06 | 普莱柯生物工程股份有限公司 | Triple live vaccine for swine transmissible gastroenteritis virus, swine epidemic diarrhea virus and swine rotavirus |
| CN103709248A (en) * | 2013-12-24 | 2014-04-09 | 北京大北农科技集团股份有限公司动物医学研究中心 | Anti-TEGV (Transmissible Gastroenteritis Virus) high-immune serum and preparation method thereof |
| CN103777010A (en) * | 2014-01-27 | 2014-05-07 | 深圳市宝舜泰生物医药股份有限公司 | ELISA (Enzyme-Linked Immuno Sorbent Assay) detection kit for porcine epidemic diarrhea viruses and preparation method thereof |
| CN106267195A (en) * | 2016-08-30 | 2017-01-04 | 天津市中升挑战生物科技有限公司 | A kind of pig virus diarrhoea triple antigen antibody complex and preparation method thereof |
| CN113929775A (en) * | 2021-10-26 | 2022-01-14 | 国药集团动物保健股份有限公司 | Yolk antibody for preventing and/or treating porcine diarrhea, preparation and preparation method thereof |
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| CN101948536A (en) * | 2010-05-10 | 2011-01-19 | 洛阳普莱柯生物工程有限公司 | Porcine transmissible gastroenteritis virus (PTGEV) and porcine epidemic diarrhea virus (PEDV) dual yolk antibody and preparation method thereof |
| CN101948536B (en) * | 2010-05-10 | 2013-07-03 | 普莱柯生物工程股份有限公司 | Porcine transmissible gastroenteritis virus (PTGEV) and porcine epidemic diarrhea virus (PEDV) dual yolk antibody and preparation method thereof |
| CN102949718A (en) * | 2011-08-26 | 2013-03-06 | 普莱柯生物工程股份有限公司 | Triple live vaccine for swine transmissible gastroenteritis virus, swine epidemic diarrhea virus and swine rotavirus |
| CN102949718B (en) * | 2011-08-26 | 2015-06-17 | 普莱柯生物工程股份有限公司 | Triple live vaccine for swine transmissible gastroenteritis virus, swine epidemic diarrhea virus and swine rotavirus |
| CN103709248A (en) * | 2013-12-24 | 2014-04-09 | 北京大北农科技集团股份有限公司动物医学研究中心 | Anti-TEGV (Transmissible Gastroenteritis Virus) high-immune serum and preparation method thereof |
| CN103709248B (en) * | 2013-12-24 | 2019-07-16 | 北京大北农科技集团股份有限公司动物医学研究中心 | A kind of anti-swine infectious enterogastritis virus hyper-immune serum and preparation method thereof |
| CN103777010B (en) * | 2014-01-27 | 2016-04-20 | 深圳市宝舜泰生物医药股份有限公司 | porcine epidemic diarrhea virus ELISA detection kit and preparation method thereof |
| CN103777010A (en) * | 2014-01-27 | 2014-05-07 | 深圳市宝舜泰生物医药股份有限公司 | ELISA (Enzyme-Linked Immuno Sorbent Assay) detection kit for porcine epidemic diarrhea viruses and preparation method thereof |
| CN106267195A (en) * | 2016-08-30 | 2017-01-04 | 天津市中升挑战生物科技有限公司 | A kind of pig virus diarrhoea triple antigen antibody complex and preparation method thereof |
| CN106267195B (en) * | 2016-08-30 | 2020-01-21 | 天津市中升挑战生物科技有限公司 | Triple antigen-antibody complex for porcine viral diarrhea and preparation method thereof |
| CN113929775A (en) * | 2021-10-26 | 2022-01-14 | 国药集团动物保健股份有限公司 | Yolk antibody for preventing and/or treating porcine diarrhea, preparation and preparation method thereof |
| CN114525261A (en) * | 2021-12-27 | 2022-05-24 | 武汉科前生物股份有限公司 | Porcine epidemic diarrhea and porcine rotavirus combined inactivated vaccine and preparation method thereof |
| CN114525261B (en) * | 2021-12-27 | 2023-09-19 | 武汉科前生物股份有限公司 | Porcine epidemic diarrhea and porcine rotavirus bivalent inactivated vaccine and preparation method thereof |
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