CN1957711A - Biologic bactericide and preparation method - Google Patents
Biologic bactericide and preparation method Download PDFInfo
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- CN1957711A CN1957711A CN 200510117421 CN200510117421A CN1957711A CN 1957711 A CN1957711 A CN 1957711A CN 200510117421 CN200510117421 CN 200510117421 CN 200510117421 A CN200510117421 A CN 200510117421A CN 1957711 A CN1957711 A CN 1957711A
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Abstract
A biologic bactericide is prepared from the fermented liquid of the variant 0116 of Streptomyces roseoflaver, phenylethylphenol polyoxyvinylether, sodium acetate and acetic acid through slant culture, shaker culture, seeding tank culture, fermentor culture, depositing the fermented liquid, removing residual dregs, adding stabilizer and packing.
Description
Technical field:
The present invention relates to a kind of biological bactericide that fruit is dredged disease of preventing and treating, the invention still further relates to the preparation method of this biological bactericide.
Background technology
Traditional bactericide overwhelming majority all is to use chemical pesticide, utilizes the action of chemicals in the antibiotic to suppress killing pathogenic bacteria.This series products certainly will bring drug injury problems such as pollution and residue of pesticide in producing and using.Can cause germ, insect pest pesticide resistance to occur as the long-term frequent chemical pesticide that uses, the drug effect of chemical pesticide itself is reduced gradually, just need to strengthen dosage for reaching the sterilization purpose.Like this, not only can improve agricultural cost, persticide residue is increased sharply, very unfavorable to health, serious environment pollution again; Because the wide spectrum kill activity of chemical pesticide, the natural enemy that also kills off the insect pests in a large number after using destroys the ecological balance.In order to overcome above-mentioned shortcoming, people have researched and developed many biopesticides, and wherein many biopesticides have the better prevention ability, can also protect pest natural enemy, and are to the person poultry harmless, free from environmental pollution.But the biopesticide bactericide of current domestic institute development and production is because it prevents and treats mechanism is that metabolite by microorganism active somatic cell self suppresses harmful microorganism, its preparation technology is to be purpose to extract viable bacteria in preparation process, and is the most just to extract in the viable bacteria amount.In practice process, find, the biopesticide bactericide exists control poor stability, action effect to wait shortcoming slowly, facts have proved in a large number, cause the slow reason of above-mentioned action effect to be that it is closely-related with the environment of using ground that microbial bactericide is used the survival rate and the metabolism power thereof of its active somatic cell of back, environmental differences will be brought the difference on the action effect.
Summary of the invention
The objective of the invention is at the deficiencies in the prior art, provide that a kind of stability is high, action effect is fast, be the biological bactericide of main Fungicidal substance composition with the mutagenic strain and the antibacterial in the metabolite thereof of rose yellow streptomycete (Streptomyces.roseoflaver).
Another object of the present invention provides the preparation method of this biological bactericide.
The microorganism that the present invention uses is to adopt mutagenic strain 0116 bacterial strain of the rose yellow streptomycete (Streptomyces.roseoflaver) that newly filters out to carry out the novel bacterial of activation culture.This novel bacterial entrusts that " China Committee for Culture Collection of Microorganisms's common micro-organisms " center " carries out preservation, strain name: rose yellow streptomycete 0116, deposit number: CGMCCNO.1471 see that preservation (survival) proves.
The objective of the invention is to realize with following technical scheme: a kind of biological bactericide, its component and content are: the mutagenic strain 0116 bacterial strain fermentation liquor 93---96% of rose yellow streptomycete (Streptomyces.roseoflaver); Phenethyl phenol polyethenoxy ether 3---5%; Sodium acetate (CH
3COONa) 0.5---1%; Acetic acid (CH
3COOH) 0.3---1%;
A kind of preparation method of biological bactericide, technological process comprises: the inclined-plane bacterium activation culture of novel bacterial, and------seed tank culture---fermentation tank culture---zymotic fluid precipitation is removed residue-interpolation stabilizing agent-finished product packing in the shaking table cultivation, it is characterized in that: a. is carrying out slant strains when cultivating operation, is to adopt mutagenic strain 0116 bacterial strain of the rose yellow streptomycete (Streptomyces.roseoflaver) that newly filters out to carry out the novel bacterial of activation culture;
B. when carrying out the fermentation tank culture operation, the mixing speed in the fermentation tank is: leave standstill 4h after the inoculation earlier, 4-12h is 140 commentariess on classics/min, progressively is increased to 250 commentariess on classics/mn behind the 12h, to the 120h bacterial metabolism abundant after, stop stirring;
C. when carrying out the fermentation tank culture operation, the temperature in jar is controlled to be: start back 0---20h is 29 ± 3 ℃, and 20h is 32-34 ℃ later on, remains 8-15 ℃ after the filtration.
D. when carrying out the fermentation tank culture operation, a jar interior pH value is controlled to be: the pH value of 0-24h is 6.4-6.7, and putting jar pH value that extraction is preceding is 7.5-7.8, and shutting down post precipitation accent pH value is 4-4.5.
The field control effectiveness test of biological bactericide (hereinafter to be referred as 0116 aqua)
Material and method
Test material
Subjects is a powdery mildew.
Trial crops is cucumber and muskmelon, selects susceptible variety for use.
Test site
Test site is arranged in the plot that Han Cunhe falls ill over the years and weighs.Carry out small plot experiment in 2003 and 2004, and carrying out field experiment in 2005.The field trial in totally 3 years.
Reagent agent and use multiple
2004: biological bactericide: 100 times and 200 times; 300 times and 600 times in aqua; Contrast medicament: celestial living 600 times;
2005: biological bactericide: 300 times of contrasts of aqua medicament: celestial living 600 times;
Experimental scheme
Each concentration repeated four times in 2004, amounted to 9 processing, 36 sub-districts, each sub-district area 25m
2And require morbidity heavy and even.
Three booths in 2005 repeat 3 times.Each processing all compares with clear water.
Spraying time and method
Press above-mentioned processing method and concentration dispenser at premorbid, every 7 days once, totally 3 times.Spraying.
Control time and number of times
Initial inquiry: investigation immediately before the chemicals treatment.
Second and third time investigation: before second and third time spray medicine, investigate.
Final investigation: the 8-10 after medication for the third time days.
Investigation method
Every sub-district is got 4 points at random, whole blades of every some investigation two strains, and every scab accounts for the percentage classification record of leaf area.Stage division:
0 grade: no scab;
1 grade: lesion area accounts for whole leaf area below 5%;
3 grades: lesion area accounts for whole leaf area 6-10%;
5 grades: lesion area accounts for whole leaf area 11-20%;
7 grades: lesion area accounts for whole leaf area 21-40%;
9 grades: lesion area accounts for whole leaf area more than 40%.
The preventive effect computational methods
Disease index calculates routinely.
Control efficiency (%)=(after the dispenser of blank district disease refer to-the treatment region dispenser after disease refer to)/dispenser of blank district after disease refer to * 100
Carry out significance test of difference and correlated results is analyzed according to test situation.
Direct influence to crop
Crop is had or not poisoning, the type and the degree of record poisoning.Crop is had or not other useful influences, as stimulating growth etc.
Result and analysis
2004 0116 aqua field control powdery mildew of cucumber result of the tests
Tested at Han Cunhe in 2004.300 times and 600 times liquid sprayings of 0116 aqua employing 3 times, each 7 days at interval, the effect of 300 times of liquid of 0116 aqua and chemicals 62.25% celestial living 600 times of sprayings was quite as shown in table 1 as a result.
Table 1 biological prevention and control agent in 2004 is prevented and treated the greenhouse test result of powdery mildew of cucumber
| I | II | III | IV | Average preventive effect (%) | The significance of difference | |||||
| Disease refers to preventive effect (%) | Disease refers to preventive effect (%) | Disease refers to preventive effect (%) | Disease refers to preventive effect (%) | |||||||
| Aqua | 0 | 100.0 0 | 0.00 | 100.00 | 0.12 | 99.38 | 0.14 | 99.24 | 99.72 | a |
| Celestial giving birth to | 0 | 100.0 0 | 0.45 | 96.66 | 1.68 | 91.60 | 1.10 | 94.25 | 96.43 | a |
| Clear water (CK) | 10.08 | 0.00 | 13.47 | 0.00 | 20.00 | 0.00 | 19.10 | 0.00 | 0.00 | b |
2005 0116 aqua field control powdery mildew of cucumber result of the tests
On the basis of result of the test in 2004, carried out the test of enlarged-area and crop in 2005 again at Han Cunhe.0116 aqua adopts 300 times of liquid sprayings 3 times, and each 7 days at interval, it is as shown in table 2 that 300 times of liquid of aqua significantly are better than the celestial effect of giving birth to 600 times of sprayings of chemical pesticide 62.25% as a result.
Poisoning is not all found in test in 2 years, and leaf color ratio blank is dark green, has delayed aging.
Table 2 biological prevention and control agent in 2005 is prevented and treated the greenhouse test result of powdery mildew of cucumber
| I | II | III | IV | Average preventive effect (%) | The significance of difference | |||||
| Disease refers to preventive effect (%) | Disease refers to preventive effect (%) | Disease refers to preventive effect (%) | Disease refers to preventive effect (%) | |||||||
| Agent water | 0.18 | 96.41 | 0.35 | 93.41 | 0.12 | 97.7 5 | 0.28 | 94.5 2 | 95.52 | a |
| Celestial giving birth to | 0.90 | 82.03 | 0.59 | 88.89 | 0.77 | 85.5 8 | 1.02 | 80.0 4 | 84.14 | b |
| Clear water (CK) | 5.01 | 0.00 | 5.31 | 0.00 | 5.34 | 0.00 | 5.11 | 0.00 | 0.00 | c |
Above test data shows:
Adopt the prescription of the technology of the present invention and the biological bactericide of explained hereafter to use as agricultural chemicals is equally convenient.
The speed that takes effect is fast, because effective ingredient is rich in biochemical product, can directly act on controlling object, thus generally speaking its action effect faster than viable bacteria.Affected by environment less during use, control efficiency is also just stable than common biopesticide.Be used for the powdery mildew of foliar spray control crops, effect is remarkable.
Embodiment 1
The preparation method of this biological bactericide, technological process comprises: the inclined-plane bacterium activation culture of novel bacterial, and------seed tank culture---fermentation tank culture---zymotic fluid precipitation is removed residue-interpolation stabilizing agent-finished product packing in the shaking table cultivation, its specific requirement is: carrying out slant strains when cultivating operation, is to adopt mutagenic strain 0116 bacterial strain of the rose yellow streptomycete (Streptomyces.roseoflaver) that newly filters out to carry out the novel bacterial of activation culture.Its culture medium prescription mainly comprises starch, corn steep liquor and an amount of potassium dihydrogen phosphate (KH
2PO
4), sodium chloride (NaCl), magnesium sulfate (MgSO
4), ferrous sulfate (FeSO
4), calcium carbonate (CaCO
3), all the other are clean water.Starch in its prescription, the percentage by weight that the corn steep liquor dry matter accounts for medium is: starch is 1.5%, the corn steep liquor dry matter is 2%.
When carrying out the fermentation tank culture operation, the mixing speed in the fermentation tank is: leave standstill 4h after the inoculation earlier, 4-12h is 140 commentaries on classics/min, progressively is increased to 250 commentaries on classics/mn behind the 12h, to the abundant metabolism of bacterium, stops to stir.
When carrying out the fermentation tank culture operation, the temperature in jar is controlled to be: start back 0--20h is 27 ℃, and 20h is 32 ℃ later on, remains 15 ℃ after the filtration.
When carrying out the fermentation tank culture operation, transfer a jar interior pH value with NAOH or 5% salt sour water, control it as: the pH value of 0-24h is 6.4, and the pH value of putting before jar extracts is 7.5, and shutting down post precipitation accent pH value is 4.
Prepare 1000 kilograms of biological bactericides, concrete grammar is: 950 kilograms of mutagenic strain 0116 bacterial strain fermentation liquors that take by weighing rose yellow streptomycete (Streptomyces.roseoflaver); 40 kilograms of phenethyl phenol polyethenoxy ethers; Sodium acetate (CH
3COONa) 5 kilograms; Acetic acid (CH
3COOH) 5 kilograms; Join in 1000 liters of modulation kettles, be stirred to sodium acetate and dissolve fully.Be packaged into finished product by product requirement.
Embodiment 2
The preparation method of this biological bactericide, technological process comprises: the inclined-plane bacterium activation culture of novel bacterial, and------seed tank culture---fermentation tank culture---zymotic fluid precipitation is removed residue-interpolation stabilizing agent-finished product packing in the shaking table cultivation, its specific requirement is: a, carrying out slant strains when cultivating operation, is to adopt mutagenic strain 0116 bacterial strain of the rose yellow streptomycete (Streptomyces.roseoflaver) that newly filters out to carry out the novel bacterial of activation culture.Its culture medium prescription mainly comprises starch, corn steep liquor and an amount of potassium dihydrogen phosphate (KH
2PO
4), sodium chloride (NaCl), magnesium sulfate (MgSO
4), ferrous sulfate (FeSO
4), calcium carbonate (CaCO
3), all the other are clean water.Starch in its prescription, the percentage by weight that the corn steep liquor dry matter accounts for medium is: starch is 2.5%, the corn steep liquor dry matter is 3%.
When carrying out the fermentation tank culture operation, the mixing speed in the fermentation tank is: leave standstill 4h after the inoculation earlier, 4-12h is 140 commentaries on classics/min, progressively is increased to 250 commentaries on classics/mn behind the 12h, to the abundant metabolism of bacterium, stops to stir.
When carrying out the fermentation tank culture operation, the temperature in jar is controlled to be: start back 0--20h is 29 ℃, and 20h is 33 ℃ later on, remains 8 ℃ after the filtration.
When carrying out the fermentation tank culture operation, transfer a jar interior pH value with NAOH or 5% salt sour water, control it as: the pH value of 0-24h is 6.6, and the pH value of putting before jar extracts is 7.6, and shutting down post precipitation accent pH value is 4.3.
Prepare 1000 kilograms of biological bactericides, concrete grammar is: 930 kilograms of mutagenic strain 0116 bacterial strain fermentation liquors that take by weighing rose yellow streptomycete (Streptomyces.roseoflaver); 60 kilograms of phenethyl phenol polyethenoxy ethers; Sodium acetate (CH
3COONa) 5 kilograms; Acetic acid (CH
3COOH) 5 kilograms; Join in 1000 liters of modulation kettles, be stirred to sodium acetate and dissolve fully.Be packaged into finished product by product requirement.
Embodiment 3
The preparation method of this biological bactericide, technological process comprises: the inclined-plane bacterium activation culture of novel bacterial, and------seed tank culture---fermentation tank culture---zymotic fluid precipitation is removed residue-interpolation stabilizing agent-finished product packing in the shaking table cultivation, its specific requirement is: carrying out slant strains when cultivating operation, is to adopt mutagenic strain 0116 bacterial strain of the rose yellow streptomycete (Streptomyces.roseoflaver) that newly filters out to carry out the novel bacterial of activation culture.Its culture medium prescription mainly comprises starch, corn steep liquor and an amount of potassium dihydrogen phosphate (KH
2PO
4), sodium chloride (NaCl), magnesium sulfate (MgSO
4), ferrous sulfate (FeSO
4), calcium carbonate (CaCO
3), all the other are clean water.Starch in its prescription, the percentage by weight that the corn steep liquor dry matter accounts for medium is: starch is 2.5%, the corn steep liquor dry matter is 3%.
When carrying out the fermentation tank culture operation, the mixing speed in the fermentation tank is: leave standstill 4h after the inoculation earlier, 4-12h is 140 commentaries on classics/min, progressively is increased to 250 commentaries on classics/mn behind the 12h, to the abundant metabolism of bacterium, stops to stir.
When carrying out the fermentation tank culture operation, the temperature in jar is controlled to be: start back 0--20h is 32 ℃, and 20h is 34 ℃ later on, remains 15 ℃ after the filtration.
When carrying out the fermentation tank culture operation, transfer a jar interior pH value with NAOH or 5% salt sour water, control it as: the pH value of 0-24h is 6.7, and the pH value of putting before jar extracts is 7.8, and shutting down post precipitation accent pH value is 4.5.
Prepare 1000 kilograms of biological bactericides, concrete grammar is: 960 kilograms of mutagenic strain 0116 bacterial strain fermentation liquors that take by weighing rose yellow streptomycete (Streptomyces.roseoflaver); 30 kilograms of phenethyl phenol polyethenoxy ethers; Sodium acetate (CH
3COONa) 5 kilograms; Acetic acid (CH
3COOH) 5 kilograms; Join in 1000 liters of modulation kettles, be stirred to sodium acetate and dissolve fully.Be packaged into finished product by product requirement.
Claims (2)
1, a kind of biological bactericide is characterized in that, its component and content are: the mutagenic strain 0116 bacterial strain fermentation liquor 93---96% of rose yellow streptomycete (Streptomyces.roseoflaver); Phenethyl phenol polyethenoxy ether 3---5%; Sodium acetate (CH
3COONa) 0.5---1%; Acetic acid (CH
3COOH) 0.3---1%;
2, a kind of preparation method of biological bactericide, technological process comprises: the inclined-plane bacterium activation culture of novel bacterial---the shaking table cultivation---seed tank culture---fermentation tank culture---zymotic fluid precipitation is removed residue-interpolation stabilizing agent-finished product packing, it is characterized in that:
A. carrying out slant strains when cultivating operation, is to adopt mutagenic strain 0116 bacterial strain of the rose yellow streptomycete (Streptomyces.roseoflaver) that newly filters out to carry out the novel bacterial of activation culture;
B. when carrying out the fermentation tank culture operation, the mixing speed in the fermentation tank is: leave standstill 4h after the inoculation earlier, 4-12h is 140 commentariess on classics/min, progressively is increased to 250 commentariess on classics/mn behind the 12h, to the 120h bacterial metabolism abundant after, stop stirring;
C. when carrying out the fermentation tank culture operation, the temperature in jar is controlled to be: start back 0---20h is 29 ± 3 ℃, and 20h is 32-34 ℃ later on, remains 8-15 ℃ after the filtration.
D. when carrying out the fermentation tank culture operation, a jar interior pH value is controlled to be: the pH value of 0-24h is 6.4-6.7, and putting jar pH value that extraction is preceding is 7.5-7.8, and shutting down post precipitation accent pH value is 4-4.5.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2005101174213A CN1957711B (en) | 2005-11-02 | 2005-11-02 | Biologic bactericide and preparation method |
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|---|---|---|---|
| CN2005101174213A CN1957711B (en) | 2005-11-02 | 2005-11-02 | Biologic bactericide and preparation method |
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| Publication Number | Publication Date |
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| CN1957711A true CN1957711A (en) | 2007-05-09 |
| CN1957711B CN1957711B (en) | 2012-03-21 |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101919406A (en) * | 2009-06-09 | 2010-12-22 | 河北农业大学 | Wettable powder of biological bactericide |
| CN102634523A (en) * | 2011-04-18 | 2012-08-15 | 河北农业大学 | Negative control gene of streptomyces roseoflavus as well as preparation method and application thereof |
| CN103125527A (en) * | 2011-11-22 | 2013-06-05 | 河北农业大学 | Wettable powder of streptomyces roseoflavus biological bactericide |
| CN104041519A (en) * | 2013-03-13 | 2014-09-17 | 河北农业大学 | Streptomyces roseoflavus microbial fungicide particulate agent |
| CN105016838A (en) * | 2014-04-18 | 2015-11-04 | 河北农业大学 | Bio-control bacterium nutritional tablet |
| CN106701631A (en) * | 2017-01-19 | 2017-05-24 | 中国农业科学院植物保护研究所 | Strain of streptomyces roseoflavus and application thereof |
| CN109168959A (en) * | 2018-10-30 | 2019-01-11 | 江苏省农业科学院 | A kind of field investigation and calculation method of bag-cultivated edible fungi acarid |
-
2005
- 2005-11-02 CN CN2005101174213A patent/CN1957711B/en not_active Expired - Fee Related
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101919406A (en) * | 2009-06-09 | 2010-12-22 | 河北农业大学 | Wettable powder of biological bactericide |
| CN102634523A (en) * | 2011-04-18 | 2012-08-15 | 河北农业大学 | Negative control gene of streptomyces roseoflavus as well as preparation method and application thereof |
| CN102634523B (en) * | 2011-04-18 | 2014-04-16 | 河北农业大学 | Negative control gene of streptomyces roseoflavus as well as preparation method and application thereof |
| CN103125527A (en) * | 2011-11-22 | 2013-06-05 | 河北农业大学 | Wettable powder of streptomyces roseoflavus biological bactericide |
| CN104041519A (en) * | 2013-03-13 | 2014-09-17 | 河北农业大学 | Streptomyces roseoflavus microbial fungicide particulate agent |
| CN105016838A (en) * | 2014-04-18 | 2015-11-04 | 河北农业大学 | Bio-control bacterium nutritional tablet |
| CN106701631A (en) * | 2017-01-19 | 2017-05-24 | 中国农业科学院植物保护研究所 | Strain of streptomyces roseoflavus and application thereof |
| CN106701631B (en) * | 2017-01-19 | 2020-06-05 | 中国农业科学院植物保护研究所 | Streptomyces roseoflavus and application thereof |
| CN109168959A (en) * | 2018-10-30 | 2019-01-11 | 江苏省农业科学院 | A kind of field investigation and calculation method of bag-cultivated edible fungi acarid |
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| Publication number | Publication date |
|---|---|
| CN1957711B (en) | 2012-03-21 |
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