CN1955288A - Expression sequential label expressed in human liver - Google Patents

Abstract

The invention relates to a category of human liver expression of expressed sequence tag sequences. Use of the invention in human liver expression of expressed sequence tags, can easily find in the human liver-specific expression of expressed sequence tags, in order to find out the human liver disease-related genes, thus play an important role in the study of liver disease pathogenesis and the development of liver disease drugs.

Description

The expressed sequence tag of in human liver, expressing

Technical field

The present invention relates to biological technical field, relate in particular to the expressed sequence tag that a class is expressed in human liver.

Background technology

Liver is a digestive gland maximum in the human body.It also is the junction centre of body intracellular metabolic.According to estimates, the chemical reaction that takes place in liver has more than 500 kinds, gives corresponding treatment even experiment showed, animal after extracing liver fully, at most also can only survive more than 50 hour.This explanation liver is the requisite vitals of active that earn a bare living.The volume of blood flow of liver is very abundant, accounts for kinemic 1/4.The volume of blood flow that per minute enters liver is 1000-1200ml.The major function of liver is to carry out the decomposition of sugar, storage glycogen; Participate in the metabolism of protein, fat, VITAMIN, hormone; Detoxifcation; Secretion of bile; Engulf, defensive enginery; Make thrombin; Regulate Q volume of blood and water-electrolyte balance; Produce heat etc.Also has hemopoietic function at embryo's liver in period.

The liver eqpidemic disease is divided into hepatitis, liver cirrhosis, fatty liver, liver cancer etc.Modern medicine does not directly cause hepatocellular infringement after experiment showed, that hepatopathy virus is invaded human body, just absorbs nutrition and depend on for existence in liver cell, and duplicate in liver cell, breed." component " of its replication-competent virus are released on the liver plasma membrane as surface antigen (HBsAg), e antigen (HBeAg), cause that the human immune system produces immune response to these antigenic substances, and this reaction causes hepatocellular damage, necrosis.Immunoreactive power is decided by liver damage degree and clinical symptom weight.That this is caused by virus, immunity system is to hepatocellular war, makes about 25% patient's liver become the unbroken battlefield of the flames of war, and the damage of liver increases the weight of thus.The harm of hepatopathy never only only limits to liver itself, and it can also cause other multiple disease.Common has: (1) diabetes; (2) pancreatitis; (3) biliary tract infection; (4) functional renal failure; (5) courage sweat ephrosis; (6) glomerulonephritis; (7) renal tubule acidosis; (8) hemolytic anemia; (9) aplastic anemia; (10) myocarditis and pericarditis; (11) arteritis nodosa; (12) peptide ulceration; (13) spontaneous peritonitis; (14) sex hormone metabolism disorder; (15) thyroid function changes; (16) liver property osteopathy, or the like.Hepatopathy not only works the mischief to patient's body even life, and also is very heavy to the strike on the patients ' psychological.No matter be liver problem sufferer or virus carrier, all can be had a strong impact at life, social, aspect such as hunt for a job, enter a higher school.

But the sequence of transcriptional expression in the biological gene group (being gene) only accounts for the 3-5% of total sequence, and this part sequence is measured, and will directly cause the discovery of new gene, and obtains information the closest with the industrialization relation in the genome.The eighties in 20th century; the appearance of high-throughout automatic sequencing; make from plasmid complementary DNA (cDNA) (Complementary DNA is called for short cDNA) the many cDNA of library picked at random and clone and determine to become possibility from the dna sequence dna of a hundreds of base at non-carrier two ends.These short dna sequence dnas are called " expressed sequence tag " (Expressed Sequence Tags is called for short ESTs).The notion of expressed sequence tag the earliest by Adams etc. put forward in 1992 (Nature, 355,642-644).Sikela in 1992 and Matsubara (Sikela, et al.Nucleic AcidsRes.19,1837-1843; Matsubara, et al.Nature Genetics, 2,173-179), the research strategy of extensive complementary DNA (cDNA) (cDNA) order-checking is proposed at obtaining pressing for of a large amount of messenger RNA(mRNA) (mRNA) sequence.Venter has founded extensive expressed sequence tagging method subsequently.Its essential characteristic is exactly from being carrier with the plasmid, the purpose that structure is finished is organized complementary DNA (cDNA) (Complementary DNA, abbreviation cDNA) in the library, select many cDNA clones at random, utilize the universal primer that carries on the plasmid that DNA sequence is carried out taking turns in the cDNA two ends and measure, short thymus nucleic acid (DNA) sequence of non-carrier that is obtained from 3 ' end or a 5 ' hundreds of base of holding.In brief, expressed sequence tag is from the short DNA sequence of expressing gene fragment 3 ' end or 5 ' end, represents the part of an expressing gene to transcribe fragment.

Expressed sequence tag can be used for that novel gene cloning, human genome collection of illustrative plates are drawn, the determining etc. of genome sequence coding region.If an expressed sequence tag only occurs once in genome, it can be used as sequence tagged site (STS) so.Scheme or transcription map (expression or transcript map) expressing by the physical map that expressed sequence tag makes up.Utilize expressed sequence tag to carry out gene map and make, can accelerate the making of sequence tagged site and the chromosomal localization of new gene.Expressed sequence tag can be used as gene-specific probe, and the research that tissue-specific gene is expressed has important effect.Expressed sequence tag can also be carried out the genetic evolution relationship analysis of new gene.Expressed sequence tag can relatively can obtain the conserved sequence fragment by different sequences to all vegeto-animal genes as a kind of database, thereby obtains the genetic evolution collection of illustrative plates of gene.Just because of expressed sequence tag has so superiority, so expressed sequence tag order-checking has become the focus of many genome research mechanism.

Because the human liver specific expression gene of the present invention is relevant with some hepatic diseases, therefore, the expressed sequence tag of specifically expressing is to the pathogeny of exploration hepatic diseases and to develop the medicine of hepatopathy significant in the research human liver.

Summary of the invention

The technical problem to be solved in the present invention provides the expressed sequence tag that a class is expressed in human liver.

The technical problem to be solved in the present invention is achieved through the following technical solutions:

The invention provides the sequence of the expressed sequence tag that a class expresses in human liver, it comprises:

(a) sequence shown in SEQ ID No.1～SEQ ID No.50;

(b) complementary sequence of every sequence in the sequence shown in SEQ ID No.1～SEQ ID No.50;

(c) with the sequence shown in SEQ ID No.1～SEQ ID No.50 in every sequence the sequence of at least 70% homology is arranged, and

(d) one or several combination in above-mentioned (a)～(c).

Preferably, described sequence comprises having SEQ ID No.1～sequence shown in the SEQ ID No.50.

The present invention also provides a kind of probe molecule, and described probe molecule contains about 8-100 successive Nucleotide in the above-mentioned sequence.

By the expressed sequence tag of in human liver, expressing of the present invention, can seek out the expressed sequence tag of specifically expressing in human liver easily, thereby seek out human liver disease's genes involved, thereby in the medicine that hepatic diseases is treated in the pathogenesis and the exploitation of research hepatic diseases, play a significant role.

Embodiment

The present invention is further detailed explanation below in conjunction with accompanying drawing.Should be understood that these embodiment only are used to the present invention is described rather than limit the scope of the invention.The experimental technique of unreceipted actual conditions in the following example, usually according to people such as normal condition such as Sambrook, molecular cloning: laboratory manual (New York:Cold Spring Harbor Laboratory Press, 1989) condition described in, or the condition of advising according to manufacturer.

Embodiment 1

The separation of the mRNA of human liver organization

Separate tissue (Tissue isolation): liver derives from 5 adult mans, after the hepatotomy operation, liver organization is placed the freezing preservation of liquid nitrogen immediately.

The separation of mRNA (mRNA isolation): take out liver organization, grind, add the 50ml pipe that fills lysate, fully after the vibration with mortar, move into again in the glass homogenizer, move to 50ml after the homogenate and newly manage, extracted total RNA (TRIzol Reagents, Gibco, NY, USA).Identify total RNA quality with the denaturing formaldehyde gel electrophoresis.Cellulose column with band Oligo d (T) separates mRNA among total RNA, quantitatively.

Embodiment 2

The structure in cDNA library (Constuction of cDNA library)

With mRNA is template, synthetic double chain cDNA.After mending flat end, add the joint that contains the EcoRI point of contact.Behind the phosphorylation EcoRI end, use XhoI digestion with restriction enzyme 1.5 hours, carry out segment again and separate.Cross the fragment of post screening length＞500bp, use the phenol-chloroform extracting, ethanol sedimentation, the sterilized water dissolving, be connected to Uni-ZAP XR carrier (Strategene, CA9203, USA), with ZAP-cDNA Gigapack III Gold Cloning Kit (Strategene, CA9203 USA) packs, and the host bacterium is used XL 1 Blue MRF ' (Strategene, CA9203, USA) bacterium.Coated plate is also measured titre.

Embodiment 3

Order-checking and database are set up (Seqencing and Database Constructing)

Select the clone who has the external source fragment to insert in the library, amplification back extracting plasmid (QiagenGermany), with T3 and T7 universal primer as 3 ' and 5 ' end, adopt and stop thing fluorescent mark (Big-Dye, Perkin-Elmer, USA) method, (Perkin-Elmer carries out the EST large scale sequencing on USA) at ABI 377 sequenators.Sequencing result is removed the carrier sequence with FACTURA software, is transferred to the processing of carrying out next step on SUN Ultra 450 Server.All sequence informations are used the GCG software package again, and (Wisconsin group, USA) BLAST in and the existing database of FASTA software search (Genebank+EMBL) are lower than 95% sequence with no homology or homology and are considered as new gene and set up database.

Embodiment 4

The full-length clone of gene (Cloning of Full-length cDNA)

On the new gene fragment order information basis that obtains, carry out the cDNA full-length clone, carry out in two stages:

(1) " electronic cloning " (Electronic Cloning)

Search the dbEST database with new gene fragment order as probe, with overlap＞50bp, homology is at (the Expressed Sequence Tag of the expressed sequence tag more than 98%, being called for short " EST ") sequence thinks same sequence (Consensus Sequence), take out and connect with AUTOASSEMBLER software, part EST can the extension probes sequence.Whether the sequence that is extended with the STRIDER software analysis has complete open reading frame (Open ReadingFrame again, ORF), search Genbank or SwissProt whether homology to be arranged on the Nucleotide of determining this sequence and the amino acid levels, to help how differentiate resulting full length gene integrity with BLAST with other species.By the method for electronic cloning, can obtain the full length sequence of people's liver related gene usually.

(2) the terminal rapid amplifying of cDNA (Rapid Amplification of cDNAEnds, RACE)

If do not obtain complete cDNA total length yet by " electronic cloning " method, then at existing sequence 5 ' or 3 ' end design primer, (Clontech Lab, Inc carry out the long range PCR reaction in USA) in human liver Marathon-Ready cDNA library.Then to PCR product cloning, order-checking.The sequence that is extended with AUTOASSEMBLER and STRIDER software analysis has or not complete ORF, as not having, repeats said process until obtaining total length.

(3)RT-PCR

For the known sequences of 5 ' and 3 ' end,, can consider to adopt the method for RT-PCR if the centre has an intersegmental crack (gap) to obtain from existing public database or its data storehouse.At sequence 5 ' end design primer, 3 ' end primer adopts Oligo-dT, increases in the liver total RNA storehouse.Then product is cloned, checked order.Splicing at last just obtains total length.

By being used in combination above-mentioned 3 kinds of methods, can obtain the complete encoding sequence of people's liver associated protein.

Sequence table

＜110〉Research Center of Shanghai Human Genome

＜120〉expressed sequence tag of in human liver, expressing

<130>NP-10039

<160>50

<210>1

<211>387

<212>DNA

<213>Homo?sapiens

<400>1

1?atacagaaga?ggagatctcc?tcattgtata?ttttatatct?tatagaactt?tcattaatga

61?atcataggaa?tataccagct?cttcaaaaat?taagacatta?tacaaagtga?aatactaaaa

121?acagaaatat?gtgtttttct?caaataatat?gcttctcagg?aaagacttta?cagaaacatc

181?tcttcttatc?cgaaattaca?tccaaaccct?ttttttgtgt?gaagtgttgc?ttccagtcta

241?ctaaggtaat?aagcaagtaa?caagatagta?ttgtttgata?ctcttgaacg?cactctgatt

301?ttcaattcca?attttgtttg?aacctgtcag?cattttggga?tctgggcccc?gtcatgtccn

361?accgtcattt?tattcttttg?ggggacc

<210>2

<211>440

<212>DNA

<213>Homo?sapiens

<400>2

1?angacggagt?gagggggcag?ggtagttatt?aattagaaga?tacgggcaaa?acactgggat

61?ggcttcctga?caacttaaga?ggtctccgag?ttatattctg?ggttgggaaa?cactgaccca

121?gcccttattc?cttcaaggac?tctagtcatt?ggcaaggagg?attcatgagc?cccggtgaca

181?cagatggggg?ccctgctcta?tattcaactg?tccagagaag?atctagtcac?aacccctcat

241?ctcaaatgga?ctttggttca?gaaaacagaa?ctaaggaaaa?gtggtttcct?tggactcaac

301?cggtctgaat?ttcccactaa?ccacaacact?gacttcctga?ggcaagctgg?gctaatccca

361?cgtnttacag?ggcaattact?tgtacatcac?agggctgggg?gacaacacac?accaagggnt

421?gaggaattnt?ctaaaaagcc

<210>3

<211>258

<212>DNA

<213>Homo?sapiens

<400>3

1?attctgtgaa?aaaattatat?aattnataaa?attttaagct?gttgcatatn?ccttgcttca

61?acttagaata?cacttacaaa?gaagaaagtt?aaataacttt?gaggtctacc?atgaaaattt

121?aatgttttta?atcattttgt?tgtatatttt?tatagctttc?tcccataatt?ccttgggttt

181?ccttcacaga?aggcttaaat?gagtagtcaa?gtatatatat?gtctatntgg?aattnacttn

241?cctattaata?acatgttt

<210>4

<211>339

<212>DNA

<213>Homo?sapiens

<400>4

1?ctgatccatg?tagaggagga?gaacagtgca?taaaacatag?taagcactca?ataactgtta

61?cttgatatta?ctacttcatc?agagtaaata?aaaataagac?accaggaatg?aaagatttaa

121?tccaaccttc?tttcaaaacc?aaaggcaaac?tgttcattag?ccaaacccca?ccattccttg

181?tttaaataat?tgcgatcacc?tcctacctac?ctagccttgt?agccagcagt?ctctcccatt

241?catctactgt?catcagtcag?atttattttc?ctaaaaataa?tatttaacat?ttctagttca

301?ggatatgatg?aactaacttc?ctgctgcaaa?aaaaaaaaa

<210>5

<211>1299

<212>DNA

<213>Homo?sapiens

<400>5

1?caagttacgc?ggactccgtg?aagggccgat?tcaccatctc?cagagacaac?gccaagaata

61?tattgtttct?gcaaatgaac?gaattgagag?ccgacgacac?ggctgtgtat?tactgtgcaa

121?gaggggggga?tcatatagta?ccggctgctg?tcgctccctt?ccacatggac?gtctggggcc

181?aagggaccac?ggtcaccgtc?tcgtcagcat?ccccgaccag?ccccaaggtc?ttcccgctga

241?gcctctgcag?cacccagcca?gatgggaacg?tggtcatcgc?ctgcctgcct?ggtccagggc

301?ttcttcccca?ggagccactc?agtgtgacct?ggagcaaagc?ggacagggcg?tgaccgccag

361?aaacttccca?cccagccagg?atgcctccgg?ggacctgtac?accacgagca?gccagctgac

421?cctgccggca?cacagtgcct?agccggcaag?tccgtgacat?gccacgtgaa?gcactacacg

481?aatcccagcc?aggatgtgac?tgtgccctgc?ccagttccct?caactccacc?taccccatct

541?ccctcaactc?cacctacccc?atctccctca?tgctgccacc?cccgactgtc?actgcaccga

601?ccggccctcg?aggacctgct?cttaggttca?gaagcgaacc?tcaggtgcac?actgaccggc

661?ctgagagatg?cctcaggtgt?caccttcacc?tggacgccct?caagtgggaa?gagcgctgtt

721?caaggaccac?ctgagcgtga?cctctgtggc?tgctacagcg?tgttccagtg?tcctgccggg

781?ctgtgccgag?ccatggaacc?atggggagac?cttcacttgc?actgctgccc?accccgagtt

841?gaagacccca?ctaaccgcca?acatcacaaa?atccggaaac?acattccggc?ccgaggtcca

901?cctgctgccg?ccgccgtcgg?aggagctggc?cctgaacgag?ctggtgacgc?tgacgtgcct

961?ggcacgtggc?ttcagcccaa?ggatgtgctg?gttcgctggc?tgcaggggtc?acaggagctg

1021?ccccgcgaga?agtacctgac?ttgggcatcc?cggcaggagc?ccagccaggg?caccaccacc

1081?ttcgctgtga?ccagcatact?gcgcgtggca?gccgaggact?ggaagaaggg?ggacaccttc

1141?tcctgcatgg?tgggccacga?ggccctgccg?ctggccttca?cacagaagac?catcgaccgc

1201?ttggcgggta?aacccaccca?tgtcaatgtg?tctgttgtca?tggcggaggt?ggacggcacc

1261?tgctactgag?ccgcccgcct?gtccccaccc?ctgaataaa

<210>6

<211>204

<212>DNA

<213>Homo?sapiens

<400>6

1?gacagatagg?acaaaattta?taaacaaaat?acaagagaaa?aagcacctgn?taagatccnt

61?taatgacaac?ttcaaagcag?tgtttcatat?tctcttacan?aatagtgcca?tccatntcca

121?agttttgcca?agtgtcacgt?aaaactgtgt?tcaatggtag?actataaaag?catctacatt

181?tctttgccag?agggttttgg?anta

<210>7

<211>539

<212>DNA

<213>Homo?sapiens

<400>7

1?cttctanctt?tgcaaacttt?actgaaattt?tctttcattg?gatttttttt?aaagtaagag

61?taaatagaga?acttcaggat?aatctaatag?gcgtattaaa?gagaacacct?tgttataaaa

121?ataaagtatt?acatgggttg?agtgttttta?ctttttaaat?ttttcctgtt?tcaatcagct

181?tcttcagatt?aaacagtata?tttctgtaca?catcaaaagg?agacatacat?gtatttgttt

241?aataaaatta?ctataggaca?taagtactcc?ttacagcaca?gtctctgtta?acagttgcat

301?ggaatttaca?catatttgag?gcatccngcc?attatctttg?gggaggatat?cccggggctt

361?aaccaaattt?tatccccagc?cnggaaattt?accggtggaa?atttcctaaa?actaggtcct

421?cttnggggtt?tccataaacc?tggtaacctt?aatccaattt?aaatccanta?ctctaccggg

481?ttccctggct?ccaaccactt?gggggcttgg?gggngaggct?ggggggggtg?tgaggcctt

<210>8

<211>518

<212>DNA

<213>Homo?sapiens

<400>8

1?atgaaaagat?gtttgatgtt?tatttccacc?ttgcactcag?gtctgagcca?caagtacatt

61?aagacattga?atggtatcac?ccagggaata?cgtaaccaga?caacacacaa?gactgagatg

121?cacaagtggt?ggtggtggta?attcacgcag?aaggaaccag?acagtaaaac?aaaaattgcc

181?caacacacca?aatgatcaaa?tccgccacct?ctaggatagg?gcaaacttga?ttgctggggt

241?taaggaaccc?tagaggtctg?tttaaggtgg?ggcagaggag?gggttcttca?gctttaggct

301?tgtccctgac?atcttaactt?gcccaggcaa?gcacttgtta?catattaatt?tttccttgag

361?ggaacccagg?tcctttaggt?ggggggaggg?ggtattccct?ttttgaccca?gatctttatn

421?gggcttttaa?tttggggtca?gggtttttgc?caaacctttc?aaggccttcc?cattaaccca

481?agctaggggg?ggnggggctt?cttttattgc?tttncagg

<210>9

<211>506

<212>DNA

<213>Homo?sapiens

<400>9

1?tttttttttt?ttgctttccc?tcttcttagc?cccttcccta?aatcctaggg?tgntgaaatc

61?tcaatatacc?ttcttctttt?cccagacttg?gactaaaatt?cccttcttgg?tcttccatct

121?ctgaagatta?agagcctggn?actagactgc?ccctcagcct?gagtcaagga?ttctccatat

181?ctgtgtagag?tcttccaggc?agcctgagga?ggcatgccat?ggntggttcc?atgagcaaag

241?atagtgagac?ccataggant?ttggagagac?aaggccaccc?tgaggaccag?antgagagct

301?ggcttcctgg?agcaggtagg?acttggattg?agttaagaaa?ggaaagtcta?gagagtaggg

361?aaggaagaca?ttccagggca?gaagaagtcc?tttgagcaag?gcactgaact?gataatatga

421?tatgaaacaa?aaagtggtct?cagaaagttt?naagctaaga?agttctaaat?ggnttttttc

481?agaagcaagt?ttaggantta?agtagg

<210>10

<211>495

<212>DNA

<213>Homo?sapiens

<400>10

1?cgtggggttn?actgatggtg?gctgctgtca?nattccaagt?ggcttatggg?ataggacaac

61?cccccaggca?cttcactgta?ggacagttag?caccaagagc?taaggttgtg?agataatgca

121?aatctggcct?gtcacctctg?cagagtacag?gttcccatac?tntgaggcag?cagcagagag

181?ggaaccacca?gagaaacagc?ntttcagant?tntctttcct?ttggtntatg?gatatgtgtg

241?tgttctagtc?tttggtgggc?aatggantct?gcagctccat?gacaatcttg?ttaagtagct

301?tatgtgggga?agtntttcag?ggtcacaagg?gccacccatt?ctaaggcttc?tcattttaat

361?ttccccaggg?nttaaggagg?acaggtgggg?ggaaagggna?aaaaccttng?cacctttgct

421?attacttnaa?ttngggattc?caggaggccc?aatccaatgg?cattntttac?cctacttttc

481?ttgggcccaa?atcca

<210>11

<211>447

<212>DNA

<213>Homo?sapiens

<400>11

1?ggtcttgctt?caggttttct?ttagaaatct?gaggtttcac?aaaacaggtt?tctatgactt

61?taactgcaca?taaaaataaa?tgtgttagtt?tggtctccaa?agctcttgaa?tccattagaa

121?taaactattc?ccccaccatg?atggtcctca?ccgtggattt?catccctttt?tnatcctcaa

181?ctgtactcac?aaatacaatg?ttaactttgc?ctgctgcatc?ctgcctttgc?ccttcatctc

241?tttgtgctgc?tggagctgtc?cacagcgatt?taggggcaga?aagagtacga?aacagatcca

301?gctttcttga?aaatagactc?ccttacctgt?ctttatgcta?gcttaccaaa?aagaacggng

361?gcttcccttt?cccaccctgg?gcaggacccg?tctgcacaaa?ggctctgggg?agggcagccg

421?gnatatctct?aaggccccng?ccttcaa

<210>12

<211>411

<212>DNA

<213>Homo?sapiens

<400>12

1?ttgagacagg?gtnttggcct?gttgcccagg?ttggagaatg?cagtggatga?cacagcgaga

61?ntccatctta?aaaaataaaa?tagantaaaa?taaaaataaa?tggccgggca?tggtggcttg

121?cacctgtaat?cccagggcac?tttgggaggc?caaagcaggc?ggatcacttg?agaaatggaa

181?aaaacaagtc?acttacaaat?gaggctgaga?caaaggntcg?gcggaaagag?ccacagaaca

241?ccaaaaacag?aagcagagnt?ggacaaatta?agcaaaacct?tacagttttg?tagtttctgc

301?ttttatctaa?cacaatgact?tcgntgtact?tcatatacct?tttganttat?acctgcttat

361?acataccttt?accctaccta?ctacttntac?ctacctcgtg?ccganttctt?g

<210>13

<211>484

<212>DNA

<213>Homo?sapiens

<400>13

1?ttgcagtctt?ttactacagg?aatgtgaaga?tatatttaaa?aatagaatac?tttaaaatca

61?ggaaatgtat?tttgctctct?tgaagaaaat?agatatgatt?tggcaaagga?taaaattagc

121?cataaatgat?aaagattttt?ttaacatctg?aacttgtcat?tagcactaag?atgagctgct

181?taccagggna?ctaagaagan?ttaacatcat?caagtttaaa?atcgatcatt?ttaaatatga

241?aggagattta?aacatctcaa?ccccatggan?tcaattgtgc?tggataacta?tccngtctca

301?ttgggatagt?ggggctcnat?ccaaattgag?gggatattca?gggaggttta?aggaggatcc

361?ngcacaggag?gaggtgagga?ccccaaatgg?ctttttttct?ntccattttc?cattttncct

421?gtttattaat?ggcctggatg?gaggttttaa?gggggntttt?ataggggggc?tttttccttt

481?tcca

<210>14

<211>489

<212>DNA

<213>Homo?sapiens

<400>14

1?acttcactta?aatctgctct?gagtctctgg?atgcctggca?ggtggagaat?tcaatcttgt

61?cgttaccagt?attcctttcc?cttctccatg?ggcttatgta?agaattctgg?gcttacacac

121?tgttggaaag?ccaggtagga?actacctccc?ccgaactctc?cattcttcca?gctgctcatg

181?atccatcaac?cttctttggg?ccacctgcta?tagcaagacc?ctcctcacag?catcattcca

241?ctgacccaca?ggctcagccc?cagggaccct?cactaggaac?aggtctccac?tatgcatagg

301?gaactcacaa?aaaccttctc?ttncatcttg?ggttctggct?ggatattcca?ggccantccc

361?ccanttnttc?atntttaaaa?cacagntggg?caggttcctt?tcccntcgtt?tccaaaacag

421?ggggggtttg?ttccagccaa?tttttctggc?aggacaccaa?agttttcacc?cgttcctttt

481?caggggagg

<210>15

<211>531

<212>DNA

<213>Homo?sapiens

<400>15

1?ttttttgttt?tttgattttg?ctttaatgat?tttaattagt?atccaacatc?aattcttaat

61?ctgtatacaa?cagttctgga?gtgacctgga?taacaatttg?tttgctcata?caccttaagt

121?actccccaaa?tagaatgtat?aaaagtacta?atttagattg?gttttgtgta?atctgtgaat

181?aaataatatt?ttacagcagt?tctctgggta?atgagagtca?ctctgtacag?atagtaggta

241?tgtacttaaa?tatttataca?tttccctctg?agctagtaag?tcattgaccc?tgaagctggg

301?aatgaggctg?ggtttgcacc?ttgggaaaac?ttaatcgttc?ccatgggtaa?cacagaggac

361?tcttgcccta?ccactttctt?ccccacatct?ttggttatat?ttaaaggcaa?ggaaattggt

421?tatttacttc?atatttaata?tggttggata?cattttcagg?ggggcacaat?tccctaacct

481?caggcccatt?cttgtggtaa?taaaatattg?ccctagggna?acccnttaaa?a

<210>16

<211>482

<212>DNA

<213>Homo?sapiens

<400>16

1?ttttttttac?agagtgaata?cagctttatt?ggatgattta?gaaaccacct?ggaatcattc

61?ttataattca?aaacaccaac?ctatcattat?gataacagta?cataagttcc?agtattttcc

121?actttgagag?aaccctggtt?tccttggctg?ctttctaaag?aggagaaaaa?gaacacctaa

181?cagggagata?gatcaacaat?gagcacattt?nagggcatag?gcaaaaagta?gggagatcag

241?ttgttatgtt?atttaggtaa?gaactgatac?agtaacagtc?aggagacaaa?tagaaactnc

301?agtgggactt?nggagacact?gcctaagtgg?gaagggtgaa?agggaagtag?gagccatnca

361?aaccaagctt?aggttngacc?atatttgatg?gacaaagtcg?taaatgggtg?tttagggagt

421?ttnttaaggn?agttaaaagg?ggaaattttt?aagnggggtn?aggctatttt?gggttttttt

481?tt

<210>17

<211>455

<212>DNA

<213>Homo?sapiens

<400>17

1?ttttcnttct?ttcttttgag?gtagagtctt?cccttatctg?ccaggctgga?gtgcaatggt

61?gcaatcagct?taccacagcc?tcaaattcct?gggctcgagt?gatcctccca?cctctgcctc

121?ttgagttgct?aggactacag?gngcacacca?attttttttt?tttttaagag?acagggtctt

181?gccatgttgt?ccaggctgag?ctcaagtgac?tcttctgcct?cagccccaca?aagtgctggg

241?attacanggc?aagagcaccn?gcacctagca?tcactgatta?ctttctaagg?ccaanttccc

301?ttttggccta?agctgggact?gtaaaattcc?cttgtggggc?anggaaattg?tantgtctca

361?atnttctttg?tatctacaat?taaaacctga?acattacanc?tagggngaat?taaaagancc

421?ctntccagng?aacanggagt?tctngcctta?ngagc

<210>18

<211>454

<212>DNA

<213>Homo?sapiens

<400>18

1?ttttttgacc?taatgaaata?ccaaatttct?tggnaggctt?actggagaca?atttttttta

61?aactagactt?aattcctgtt?ttaattattt?aaaattatta?aatcaaacca?cctgataagg

121?acagantaaa?tttaaatata?ttaatgtaaa?ntttatccaa?ctattganta?ttggnctgca

181?aacacttatt?catgaaagng?ataaattttc?ggttatcaga?tgggcagaca?gaccacatct

241?aaaaccacta?ctgaaatgat?actttanctg?tcaggantct?gaggtgacan?ggaaanttaa

301?aggccggggt?cgggtgggct?catggtctnt?aaancccaac?actttggggg?agggccaagg

361?agggggaggg?ctcgcttgag?gggcaggggg?gttttggggg?acaagcnggg?gggccaaaac

421?agggggggca?cttctggttt?ttcttacaaa?agga

<210>19

<211>478

<212>DNA

<213>Homo?sapiens

<400>19

1?gnngggnngg?tatataagtg?gttggtggat?tttaacacac?cagttattcc?tcctgtgaat

61?gcctcagaga?tggtggaaac?tgaggctgat?gggggaacaa?ctttagctag?ggaaggangg

121?tattggttct?cacatttgac?ccacactcct?cctgcccggg?gttnttcctc?tctgcctccc

181?ttccctacac?tgctgctgtc?ctctttttga?catttctatt?tatttatttt?tctgccctct

241?cagatcagca?ggagagcacc?taggtctcag?ccagctctct?atggcaggcc?tctgcagcag

301?cacttgtgaa?gtgctagtga?ttcacttcct?tctctattgc?tgacccttct?ctttcttngg

361?caggcagtcc?aggggagtgg?aaggaagggt?cggagtataa?gcagggaaag?ccagggnact

421?tcctcacatt?ttcactngga?agcacttctn?cctcttattg?gcaggncaag?ttttttaa

<210>20

<211>386

<212>DNA

<213>Homo?sapiens

<400>20

1?ttttgtcttt?taacctacac?ctttatcatt?actctaacag?atttagggct?tctctttctc

61?tacagctaag?taagggaata?tgtgcaatta?tgagacatac?aaaaaaggaa?agggaaagga

121?ctttctaagt?agcaaatctg?tgccatgaag?tagatgtggc?gtgaagatac?agagcctgag

181?gatagtaatt?ttccctgagc?cacgcacaca?ggcttttatt?tcatgccttt?tctctttctg

241?tgccgtcacc?tttgaggaaa?aacgattgca?ccttctccaa?gtctggcctt?tttaacagct

301?acagttaagt?tgggccaagg?actttcccca?gctctggaat?ataggccctt?ttgcccgact

361?nccggncttt?ttttgcggag?gactgg

<210>21

<211>418

<212>DNA

<213>Homo?sapiens

<400>21

1?ttttangttt?ttaaagactc?tgaactcatc?atacaaattc?aaatgcttta?ttggagagta

61?cctaatttga?attaatcagc?tagtctcttc?ctagaagtct?taaatacctt?ttttatttta

121?ttttatttta?ttttattttt?tttaagacag?tctttctctg?tcacccaggg?ctggaggaca

181?gtgggtgcaa?tcatagctgc?actgcagcct?ctaattccng?tgttcaatcg?atccctccca

241?ctcaggctcc?taaggagcta?gggnctacag?ggngtgtgcc?acccacaacc?caacttaatt

301?ctttgtagga?gaagaggggg?nctctctatg?gttancccaa?tctgggggcc?ttnaggtttc

361?tggggacttc?aaggcnaatc?ctcccggcct?ccccaagtgg?gggggggggg?tttnaaaa

<210>22

<211>311

<212>DNA

<213>Homo?sapiens

<400>22

1?aaacctatca?tgttaatatc?ttaaaaaaag?aaaaaataga?ggaaaaaaat?ctttaagaac

61?acttaaccat?gtttgttatt?acatgttttt?tgagaccaga?ttttatgcca?tctgtgttaa

121?cccccccaca?taagtctaca?tcaagtattt?ggttaaaagt?gctcacagca?ggaatttact

181?gaggatttaa?tgggaagtgt?tctaaaactg?ggattgttgt?aatgcacaac?tgcacaagtg

241?gggtaaattt?acaaaggaaa?aatgtacaca?taaaatgggg?tgaactttat?gggnatttta

301?aaaattacag?t

<210>23

<211>345

<212>DNA

<213>Homo?sapiens

<400>23

1?ctatattttt?aagagatgta?catcctagtc?acttgtaatc?ataagttgtc?tttaactcag

61?ctggctccag?taattaaaaa?aaaaattggg?gagatgagga?ttgtttaaaa?caaatgttaa

121?tgttttaaag?ggaaataatt?tccttacagt?ttatttttat?gtttctgctt?tttagaaaga

181?aacctttaaa?aaggatatgt?atttttgcca?tattcaaaat?aaggacttca?gtatttatgg

241?gctagggggg?tattaaggaa?aggtgggatc?tgggcccggg?cacgntggcc?tccaagcncg

301?ttaatccccg?gcacttttgg?gaaggcccag?ggtgggccag?atcca

<210>24

<211>320

<212>DNA

<213>Homo?sapiens

<400>24

1?tcttnnnctt?cagttacata?tggccaaatt?taatggcact?gaaaaggcta?acatattaag

61?atggaaaaaa?tttacaagta?atataaacat?tataatctca?attttgtttt?taaaaataac

121?ataactttac?aggttaggat?aaaatgcacc?aaatttaaca?gtgattacct?gaaggtaaca

181?gaatcatagg?caatctttat?tttttccttg?cggtacattt?ttatggatat?atacaggtat

241?tttttcataa?tcngggaaaa?aagggccttg?gaaatttata?atttttaaaa?ggncctctcc

301?ccncccaaat?agggccccat

<210>25

<211>1303

<212>DNA

<213>Homo?sapiens

<400>25

1?ctcaacatgc?caagagccag?atgaatagtt?cacacaatct?gaatttaact?gaaaaaaaga

61?gatcatgctt?tgaagcaggc?tagactggag?ctggacttta?gtggataggt?cggattctga

121?aaaattagag?cacattttag?gaagagcaac?agcacaaatt?attatgttat?caccatcttt

181?gtctttttat?ttccttagat?ttacaagcac?tagagattgt?cctatgtgac?cccgtttcct

241?attttgctgg?gaaaatggga?gcaattagaa?atctttcact?tgtttccatc?caccaccacc

301?actactttgc?ccattcccat?tactctgcct?tcctagtact?ggaattggac?tgtttctatt

361?cctacttaaa?gccagcctct?ccatttgttc?atcagatccc?ttcttacggc?ctatcaagtg

421?ttgtttcagc?aactctccct?cttcagtctt?ttttccctcc?tgactgcatt?cccattaatg

481?tataaacatc?ctcccattaa?aaaaaaacaa?aaactcaaat?cctatattcc?tttctaccta

541?tagccccatt?tctctgtttc?tctttaagga?aaactcttga?gttttcgata?ttcatatctc

601?aattcctcgt?ttttcattct?cttttaaatc?atgctgtgag?gctccaaact?catactgata

661?aatctaattc?tcatcttact?tgaatcagca?gcagcatttg?acacatgatc?aatccacttg

721?aaatattttc?ttcatttgac?tctgaggaca?catgcattca?tagttctaat?tcccatctgc

781?attctacaca?tcacagcagg?tgcaccaaac?cacacctgct?gtggactaaa?cagacttgga

841?tggcagagca?ggatccatag?gcacacaagc?taggtgtcac?attcattgat?taaatttttg

901?gatttcactt?atcaggctag?gttgagttac?agcaggtcca?cagatagtgc?tgttttgttc

961?aatgttactt?tgttataagg?ctgatgagga?aaaaaaggat?tgccagacca?gggctactgt

1021?ctgtgtggag?tttgcacgtt?ctccccatat?ctgtgtgggt?ttctccaact?attctgttct

1081?tccacatcca?aagatgtgca?tgttaggtga?actggtgtgt?ctacatggtc?ccagtatgag

1141?tgtggatatg?tgtgtgtgtg?ccctgagatg?ggatggcggc?ctgtgcaggg?ttggctcctg

1201?ccttgtgccc?tgagctgccg?ggataagctc?tggtcaccag?tgaacctgaa?ctggaataag

1261?tgagtcaaga?attatcttgt?ttttattaat?caatgtatgt?aca

<210>26

<211>605

<212>DNA

<213>Homo?sapiens

<400>26

1?tcaaatttaa?atttttagaa?tgatggtgct?actatattct?aattgttaat?ttgataattg

61?taattgtaat?ttgttaattt?gttctaataa?tattttcatg?acattattca?tttgctgctt

121?ggcttgatgg?cataacaagt?atgggttatt?taccctgaaa?aggaaacagt?atcacccttc

181?cagaatctct?atttacagct?acagaagatg?caacacacct?gtttctggac?cacttccaga

241?tcagatgtga?tgcatgcttt?agatcattta?ctttgatcta?ggaattactg?tctggcattt

301?cgtggtgtag?ggtaggtgtc?tccagcactg?tataggtgct?ggaattgaga?taagaatcaa

361?tttcaataca?aaatacattt?tttgcttaaa?nacgcaagga?tntatgaatc?aggtaaatna

421?cagggtggaa?aacaggaact?aatgttatat?atnctgtagt?tactcataat?gaanagaaag

481?aaaccaaaaa?gttccaaaat?anatgaaaga?ctgtattnct?agaatgaaaa?tatctttaga

541?tgtgatagag?ttcanacctn?aaaagnatgt?gagagcccat?gnccccagat?tgttccncca

601?cccag

<210>27

<211>1232

<212>DNA

<213>Homo?sapiens

<400>27

1 gcaggtgatc?cacccacctc?tgcctcccaa?agtgctggga?ttacaggcat?gagccaccgc

61 gcctggccag?aagaaatctt?tatcttggtg?tgcagtgtct?ggtgagggac?aaatatcatc

121 tctcttggat?ctgaatctgg?aagaattcgc?ggcactgaag?ggattttttt?ttttcagaca

181 gtatccctct?gtcgccaggc?tggagtgcag?tgccgcaatc?tcagctcacg?gcaccctctg

241 cctcccgggc?tcaagggatc?ctcctgcctc?agcctctcga?gtagctggga?ctacaggcac

301 gcgtcaccag?gcccagctaa?tttttgtatt?tttagtagag?acagggtttt?accacggtgg

361 ccaggatggt?ctcaatctct?tgacatcatg?atccacctgc?cttggcctcc?caaagggctg

421 ggattacagg?cgtgagccac?cacgcccggc?ctcactgaag?ggattttttt?aatgtcacgt

481 ggctctcaca?ggtgcggtgt?gtttgggtgc?aagtgaagat?tacgactgat?gcttaaaaac

541 aaatgtaaaa?ttccaggtgg?ttgttgctat?ggggagcagc?tttaggacaa?tctgagtggt

601 ttcagttgca?agagtgtgcg?tgtacgtcca?agtgctacag?tcaagattca?actgctggct

661 ttgagggcct?ctttaagaac?agtaatgata?acctaaggca?gtttaacagt?atggaatggt

721 taccttttag?aagttaagct?atgggcatgg?aagttcaatc?agtgcattga?agtttttcct

781 ttatctctcc?tatggttaat?ggtttctgca?gaaaaggacc?aattgatttc?tttctaaaac

841 gttgcttcag?ggtgtagaga?cctttatagg?tcatgtttca?acttacagaa?aatttttata

901 gttcaaatat?aaattaagtt?caatgtggaa?tttgtaatag?aatttcaggt?gaagtaaaat

961 ttccactttc?cttaggctgt?ttgcagtgcc?cagcaggccc?catgatatcg?agatggaagt

1021?tctgttaaag?gaggagattg?ctcagggatg?ggcagaataa?ggaatatggg?cagctcaggc

1081?taatgataca?atgattgaga?tgtagaaaga?gggtcaggca?cgggataacg?cctgtaatcc

1141?cactggcttg?ggaggcccag?acaagagaat?cgattgaggt?cagaccagcc?tggtcaacag

1201?agtgagacct?aacctgtaca?ataaaaaaaa?aa

<210>28

<211>796

<212>DNA

<213>Homo?sapiens

<400>28

1?gntgggaaaa?gtgtactaca?tgaaggatgc?ttggggcttt?gctaacctgc?tcaccgctaa

61?ccctccaagt?ctaaccatcc?ccagaggcca?cacaaaccaa?gtgactcctg?tagttcccat

121?ttgccccact?atggagtcag?ggnaaaagtg?gaatagccac?tccatgtgat?tttagcatgt

181?ttaatcattt?taacaataaa?ccaccccaca?aatggggtca?tttancctct?aaaaaaaaaa

241?aaaaaaaaaa?aaaaaaaaaa?aaaaaaaaaa?aaaaaaaaaa?aaaaaaaaaa?aaaaannnnn

301?nnnttttttt?tnnnggnnnt?ttnccggggg?aaatttaana?anccccncnn?nnnnnnnnnn

361?nnnnnnnntt?nnnnnnnnnn?nnnnnnnnnn?nnnnnnnnnn?nnttnnnnnn?ntnnnnnnnn

421?ncnnnnnnnn?nnnnnnnnnn?nnnnntnntn?nnnnnnnnnn?nnnnnncnct?ttnnnntnnn

481?nnnnnntnnn?nnnnnnnnnn?ntnnntnnnn?nnnnnnnnnn?tnnnnnnnnn?tnnnnnnnnn

541?nnnnnnnnnn?nnnnnnnnnn?nnnnnnnnnn?nnnnnnnnnn?nnnnntnnnn?ncnnnnnnnn

601?nnnnnnnnnn?nnnnnnnnnn?nnnnnnntnn?nnnnnnnntn?nnnnnnnnnn?nntnnnnnnn

661?nnnnnnnnnn?nntnnnntnn?nnnnnntcnn?nnnnnnntnn?nnnnnnnnnn?nnnnnnnnnn

721?nnnnnnnnnn?nnnnnntntn?ntnnnnnnnn?ntnntnnnnn?nnttnnnnnn?nnnnnnnnnn

781?nnnnnnnnnn?nntnta

<210>29

<211>453

<212>DNA

<213>Homo?sapiens

<400>29

1?tgtgaatagt?aattataatt?aaccctttct?aaaagaaaag?gtatatgtaa?tagtacataa

61?tagagtaaca?attgtaactc?aagtcttttt?ttttcctttt?tactcaaagc?ataaaccaga

121?cacaatacgt?tataccagat?taacatgcaa?cttaaaaaga?ggttaatgta?catactaagc

181?atggaaattc?accagtaaaa?tcttatttgt?cattttaccc?ccactacctt?gacattattc

241?ttccatttca?taatcaacaa?ggttctactt?ctggtcacct?gataaatgcc?ttgcattcct

301?ggagcttgac?atttcagtca?attatnggac?acaagaacaa?tgttacaagc?ataaagaaaa

361?aatgcatcta?cgaaacagct?ggactgcact?gctttcaatg?gaaaggtccc?cactccaacc

421?tgcccactcc?ctcccttttc?tcagcacaaa?cat

<210>30

<211>611

<212>DNA

<213>Homo?sapiens

<400>30

1?tttttcggtt?ttgctacact?ttaatgggtt?ttttttttaa?gggatttttt?ttcaggtctt

61?gtcagcaaca?tcaaacaaaa?ggtactgagt?actccacagg?gtacagagtg?ctgccaaaca

121?ccttagaaaa?attacatgac?acggagaaaa?tgcgcctctt?gctccttgaa?gagcttacag

181?tctagggatt?tgacaactca?cagtcttagg?aactgggcaa?agtaaggcaa?attcttcatc

241?ccctagagct?attgtggact?gaatcatttt?agaatttgga?attaatccaa?tcaagatgag

301?agacaagact?aaatttggct?gagaattcat?tcaggctccg?catagttttt?attaacatcc

361?gtctagtaaa?cagaatggac?ctaacagaca?actggaaagt?aaagactaga?tcccttgaag

421?tgccagggct?acaacaacct?taattgtggg?ttacttattt?ttaaaaagca?aacataccga

481?atggtatgac?tagggtgatt?acccagttta?aaaataggcc?aggtactgac?actggcattc

541?ccctcatgca?ttgcccattt?aaaatagngg?atattaaata?tgtgggctta?aacncccggg

601?ccgaatctgg?c

<210>31

<211>579

<212>DNA

<213>Homo?sapiens

<400>31

1?aaatatttaa?attttattct?taaatactat?ttattaatac?agagaaatac?tcctgctata

61?attttaagtg?acacaatagt?acaacaattg?ttaagaacaa?tccacctgtg?tcaaagagac

121?acatacacaa?acacacatcc?atatacacat?acaagcttcg?gagaaaattc?tgaaaggatc

181?tcattgtcct?agatggtggc?attatgggca?attattttgc?aaatgtcacc?cagtgaagaa

241?gcaatggtgc?ctttggaaag?tatgtagctc?ttagttaagc?ttttatctgg?aaggtgctgg

301?agaaggtctg?ggcacagagc?tggttcggct?ccatnggttt?tgtgacctgt?gccatcacat

361?aggaccccat?gttcagaagg?accccactct?tgggtttaat?gctctgctgc?cgtgtcttga

421?aattcttaat?aatttttttg?aacaagggga?tgctgcattt?tcactgtgtt?ctggggccta

481?atgattatgt?agtcaatctt?gnctggcagc?tctnctctgg?cccatcactg?gtagatgatt

541?gtgggggtgg?ttantccttt?aaaatttcca?tttaaaaaa

<210>32

<211>609

<212>DNA

<213>Homo?sapiens

<400>32

1?cccatttgga?acaagtgccc?atcaaaagct?taggagatat?aaaagatgta?caagaacaac

61?tccaagatcc?cttttaagag?tctttcatct?ttctgtaaaa?actaaaccaa?gatacataac

121?atatagagtg?tcacatatcg?catgctaatt?cagaagcaag?ctatatgaag?caataggaaa

181?cacagagccg?tcagagcggg?aaagccctgg?aggcacgagt?cagcgatgac?accacagggt

241?attcaaggag?caccaatggc?ctgcaggtgg?cagaaacaat?ggaggaacca?gcctgagtca

301?aggaggactc?actaggtaag?cttgggaaat?agggctgaag?acgtggactg?agcagtcgat

361?gggaaggctc?ggaagtcaga?catggctcac?ctgcgacagg?gggcctcagc?aagagacagg

421?tatgagggac?actgttttag?gcccatggga?ttaaaaacac?ggctgccatc?aacatttgat

481?ttcagcctat?gtaaatggtc?cggtgcaccn?gtttattctc?aacaagaact?ttaatccagg

541?gtggctccta?ccacctcnat?tcctatcatc?aaaatgggtc?aaatttggcn?aagccccgnt

601?gtttacata

<210>33

<211>723

<212>DNA

<213>Homo?sapiens

<400>33

1?tcggaggtaa?aaatttaatc?cccgttttac?aggtgagtga?agtgggataa?aatggcataa

61?ttcactcaag?atcataagct?attgagtagc?aaagacaaga?gttaagcata?aagtactcaa

121?gaacctgtgt?tttacattct?atctgaagtg?caaagaagaa?tacctgaatg?tcaaatgcag

181?ccagaactct?gggataagac?ccgaggaaca?agtcctgcag?cagcagtgct?aggaggcaga

241?agaggaccat?gggttcacat?gctcaggaag?agccatcagt?aacaacaatc?aaccacctac

301?tgagactctg?gccatgggtc?tccccggcta?taggggtaag?gtcggaggtt?taaataaatg

361?gcctctggcc?attccactgg?gcaatacaat?ctaagggaag?ctggccaaac?tttactggaa

421?cccttggcat?gtatctgtat?aatgcaaatg?tcattataca?tttacctcac?tttggtatat

481?atgtatacat?tatacatatc?cctcacnttg?tcaggaattt?aaatgtgtaa?aaatttacag

541?gctgggcatt?gtgggctcac?acgntgtaat?cccatttccc?gacnaacggg?gttggataaa

601?tgccggatan?tccatataaa?gggctttcnc?ctggacnctt?gggttcaagg?cnggtttaac

661?cccggagggg?gattaccggt?ttccgttttc?ccagantttt?nttcccataa?tctantgctn

721?ctt

<210>34

<211>648

<212>DNA

<213>Homo?sapiens

<400>34

1?ttttataaaa?gcttgttttt?ctttattaga?atactttttt?caattctgat?ttgtcacaat

61?ttagattctt?tttctaagaa?taagcagaaa?tttacaaaat?ttaattttta?tttatacatt

121?catccgttca?atacacattt?caagaaagct?gtattgcacc?ctttcagtgg?tagttacagg

181?acaaagaaac?aaaataatcc?aagagagaga?ccaacaaatg?tatatttata?acacagagta

241?ataaacacaa?ataaatgtgg?agttatttaa?gcatgtaaga?tggtacatgc?tctaccaggt

301?atgggggctt?ctctaagaca?caagatcaga?ttaaagtctt?gaaagataat?ctggggttag

361?tcaagcaggt?agaagtgtga?agagcacttt?ctgagcggaa?tctccatgtg?ccaagtctag

421?ttcaagagac?tggatagaga?ttagcaattt?ggagaatgca?atattaagaa?atggcaaagg

481?tgagcctaca?gagattccaa?agttgcatag?tgaggtttgt?gtttgagaaa?gtggccncag

541?gcagccccca?aaagagggtt?aatagaaagg?agctgaggtg?gaaatcaggc?ttgtgttcan

601?cagtgtggca?nattgggagc?atgggagatc?tgnctttnaa?ggncaagg

<210>35

<211>466

<212>DNA

<213>Homo?sapiens

<400>35

1?gtgagccagg?ctacatgcca?ggctttatgc?tactgtgttt?atttctcttt?gttacttgcc

61?agcacagtga?tactagaact?tgcagaaatt?taagggtaac?cggctattac?tttaaaatac

121?cttttcccag?gctcccactg?cagaccttca?aaatccatgt?cccccaagat?tctgtatatt

181?taaactggtg?tccagagcag?tttctatgac?ctcaaaaggt?tgggaaacat?tgccctcatg

241?ttcagaaaag?tcctttaaac?ttttcagctt?tgacttagtt?gtggagagat?gttgaaaaat

301?gccttttgtc?atcccattgc?ctgtacctca?gatatgaagg?aagaaatgac?ccaaggaggc

361?ttcgatggga?gccaaaatca?tcagagggga?gagtctcggg?agaaagatga?atctccaggg

421?caggggccaa?gggtttggtg?ggtccccccg?gctcctggcc?cctgca

<210>36

<211>487

<212>DNA

<213>Homo?sapiens

<400>36

1?tntcaagatg?caaaatngtt?cgataagctt?gagtctctcc?cactctnggc?tgcaaaaatt

61?aaaaagggct?acaacaaaat?tatagactgg?aaatttgtga?ccaaatacag?cacaggattc

121?ttagactgca?aggaagacag?cttcagagaa?aggaacaagg?agagtttact?tacacttgtt

181?tataccccta?aggagggctt?ttttgtacat?atttgttggt?aatattttga?agatgtgaag

241?tcaatgcact?ttaatccgct?ttggtatcat?ggtcccttcc?agccttccac?ctctgaggct

301?gagccatnga?ggcctctttg?ccccagggga?cagtaggaca?gttctgcctc?cagcaaaggt

361?gtgatggatc?agctttactc?acaaagctga?atttctggag?cacgcagcaa?gatcatgatt

421?ttgaaagcca?ggctttggga?ganggaaatg?tgttttcnaa?gtttctttag?cttccatgaa

481?atataaa

<210>37

<211>461

<212>DNA

<213>Homo?sapiens

<400>37

1?ttttaagagc?gccanataga?tttaaagtcc?tttngttaat?ttaggaactt?aaaaccgtgt

61?gatgtatagt?ttggtttgct?cgtaaaatta?ctaagtgtgg?catatttagt?gctgtgaaag

121?cttggggtaa?aaggaaaaaa?atcagtgaga?attttcttac?aagtctttag?gggaaaatgt

181?atttttccgg?ctttttccca?cacatgccag?gccctccgca?nctggggttg?gcgctggtcg

241?ttaccctcag?ccccaattac?agctgtgctg?gaagctggga?gtgggtgctg?gggctggggg

301?attgcgggag?tagggcnctg?gtggggtttt?ggtaaaacca?ggtncaggnc?ttatctccag

361?agggggancc?cancccattc?tgggagctca?nccctgncgg?aaacccaggc?cctnaagant

421?cgggcagtnc?atctctgntc?cctcccacag?tgagacaagg?g

<210>38

<211>544

<212>DNA

<213>Homo?sapiens

<400>38

1?tttttttttt?cacgtgaaaa?aaataattta?ttacagactc?ttttacacat?taacatggaa

61?catttataca?tatatcgatg?tgctgatatg?aaatactaaa?tttaaaggca?aacattttta

121?cacaaaagta?gttgcactct?attttataaa?gatagatatt?aataagttat?cagagacatt

181?taagagctag?aggccaatta?ttccaacagt?aatgcattct?atgctgaaag?taaactaagt

241?tttctgaaca?tgatgtcctg?gatataatca?cattcttcta?agctaaggaa?agggagctca

301?tttctgggaa?tacaaggcca?agaagggctc?taacagcagt?atcccagcag?tgtgtttcca

361?gatttattct?tggatgtggt?tggagcgccc?aacatttagc?ctgaactaat?gtaacagctc

421?aatgtgaaac?aatggcagct?ttctggtaan?agctgcctgt?gggttaatga?gatttaatac

481?agggggatac?agttaccaat?gatagncttt?taggaggaat?tataattggc?catatgattt

541?ggaa

<210>39

<211>481

<212>DNA

<213>Homo?sapiens

<400>39

1?atttatatat?acctcttcna?ccattcattg?gctggagtag?ttgaacagac?atcagcaggc

61?acaatgctag?gcaggcactg?gagattcagt?gatgaataaa?acaaaactga?cctggaacct

121?ggcctcctga?agcttctctt?catctgtcta?aattcctttc?tttcaaggcc?cagtgcaagt

181?gacaccttca?tcaggaaggg?cttccacatg?attctatctg?caaatctccc?ttttctgaac

241?tattatgctt?atagtctgta?ccaggccaca?tgacatatgt?tattttgtgg?cattctagaa

301?ttattccaat?cttttcattc?ttataattgg?tgtgttgtat?ncaataaata?atgttgttat

361?tccagttttc?atcactggtc?acgcaagagt?cctctcttna?ttatatttta?tccccatagt

421?tcaccccgtt?tcctccccat?cctaagtaac?tatgaccata?cctaaatata?natctaatta

481?g

<210>40

<211>325

<212>DNA

<213>Homo?sapiens

<400>40

1?aacttcaact?tgttcacaat?agctgagagt?tactagattt?taatctagag?ttactggatt

61?ttaatctcta?gagttaacta?gatttaaaac?tagagttacc?actgcccatt?ttataaaccc

121?ttaggtgata?atgagaggca?caaaggaatg?ctgcagaaag?acacatccat?ggaggctgag

181?ttttgcaaat?aaaacctgct?tcaacatgga?accctccagt?agcctttatg?taaatgtagg

241?acttaagtgg?aaagagaagg?aggataaata?ttctagcagg?tgctgcagaa?tgcactgaat

301?aggctgaaat?gacacacgtt?ttttt

<210>41

<211>418

<212>DNA

<213>Homo?sapiens

<400>41

1?taaatagttg?tgaacataac?tattgtgaat?actatgttat?atgttatagt?tgtgaatgta

61?aatagtattc?acaattgtga?atacaatgta?aatagctttc?acaattgtga?atacaatgta

121?agtagctatg?taaatagtga?atacaatgta?aatagctatg?taaatagttg?ttatgctgat

181?tagggaataa?tgagaaggaa?naaaagtata?catattcagt?acagatgcag?tcatcttttt

241?ttcccctcga?acatttttga?tccacaattg?gttgaatcca?cagatgggga?acacacagtc

301?ttggtaaatt?taaccaacaa?ggagggtaaa?cgcatcccaa?cagggaaggt?aaactgncac

361?atccatgcag?tacctcttgg?aggggcatca?ctggtttata?ggcttcaatt?acagtgga

<210>42

<211>546

<212>DNA

<213>Homo?sapiens

<400>42

1?tttttttttt?ctttaatttt?taaaataaac?ttttattttc?aaacaatttt?agacttacaa

61?aagagttgca?aagataatac?agagtaattg?gttttatagc?atcaatttaa?gcctgttctg

121?atcttcttga?tctattttat?cactttagga?taatgaaaaa?taagttcagg?tagaggagct

181?aggctgggct?tggtggctca?cacctgtaat?cccagcactt?tgggaggcag?aggtgggtgg

241?attgtttgag?cccagggaat?ttgagaccag?cctggggcaa?cataggcgaa?aacccatctt

301?ctacaaaaaa?ntacaaaaat?taggccgggt?ntgggtgggc?acattacctg?tgggtttcca

361?gcttacttaa?gggagggctt?gagggcaggg?aggaacttgc?ctggaggcac?aaggaggttc

421?atgggctngc?agttgaggcc?ntgatttgtn?gccantggca?nttccagncc?ggggtngaca

481?gantgagant?ttgtctnaaa?aaantaaaaa?accaaacaaa?ncacacacac?acttncacac

541?aaaccc

<210>43

<211>580

<212>DNA

<213>Homo?sapiens

<400>43

1?tttttttttt?gatttatgaa?aaataaattt?attacataac?accttgtttt?taacaatgtt

61?caattatttt?ttattcagaa?tacttgtgtc?attcatgtaa?aatagtttga?tacagtacat

121?tgtatgttat?aggttttttt?ttcctcaaaa?attctaaggc?tctcctactc?cttctccttt

181?gctgaagtaa?gggcacccta?aacaacgtgc?gaacaagttt?tcaaaaggga?aaattaactg

241?gaacggaaat?tttaataggc?actacaccaa?ccagctttaa?aatcagggac?gaaaatattt

301?gaattgggat?ggccggcacc?tttttaaggg?aagttattca?ttcatggctt?tttttcatct

361?ttaaaggcat?ggcttcacaa?atcaggcaac?accaacgggg?aaaaattaaa?acactgtcnt

421?atgggacaat?ccntttaggg?ttgttttggn?tggaacccaa?cggggttttt?atncatggac

481?aacntttttg?attactttcc?ataggcggnt?ttaatacccg?tcagggcnag?ggtttggaat

541?anaacaactt?tagtgggggg?ttntctgggg?gggttttgaa

<210>44

<211>319

<212>DNA

<213>Homo?sapiens

<400>44

1?gtgtgaatta?acaacgttgg?cctctcggat?tcccaaaact?ttaaaagatt?aataatatcc

61?agtattggtt?gggattataa?gcagatatga?tatttcactg?atagagacca?gcatgactgt

121?ctcaattgtt?aaactattga?aatgcatctg?tgtcaatggt?aaatagtatt?attgtaagta

181?caacactaaa?attaataaat?tgtgtaaatt?ctaaaaaaaa?aaaaaaaaaa?aaaaaaaaaa

241?aaaaaaantt?cctgcggccg?caggcttttt?ccctttggtg?gggggttaat?tttgggcttg

301?ggcnctgggc?cgtcgtttt

<210>45

<211>444

<212>DNA

<213>Homo?sapiens

<400>45

1?aaagattccc?nnnnttttta?tttctggaac?tgtacaccag?gtattaaagt?acaacaaata

61?caaaataatg?ctcaaaaaaa?tcagtgttta?tgtacaaata?ttaaaatcaa?tgcaatagca

121?acttcctctt?gaacatctga?tactgaaact?tgttctgatt?gtcactaatt?tatctcatac

181?tcacagggta?cttatttcaa?actgggacaa?ttggaatcac?tggtcatcta?agaggaatat

241?taatatctac?catatttaac?aataaaacta?atagtttcct?ccatttagtg?aaaaaattag

301?ggaacttttt?aaaaaacata?ggtaacgtca?atattttatt?aaattatttc?aatttccntt

361?tgtgggccat?gtgctttgga?aactcggggc?aaacattctc?cntgggnggt?cgggttnttt

421?gttaggttac?ttcnagttgg?attt

<210>46

<211>407

<212>DNA

<213>Homo?sapiens

<400>46

1?aatacaaana?tngtctcaca?tacttgaact?cctgtatgta?agcaatcctc?ctgctttggc

61?ctcccaaagc?gctgggatta?taggcatgaa?ccaccacgcc?cagcctatta?ctgaaatttt

121?aatgatgatg?acaacctcat?gacaatccca?aagtactagt?atatccattt?tactgatgag

181?aaaagcacag?ctaatatgca?gtaaatctag?tttctgaatt?cagggcattc?ttattccaga

241?gcccatgcta?ttaattccta?tactatactg?tctctatata?aggcatgtag?gcatattaat

301?ctttttaagg?ggggggagta?ttaggcaggg?cccacanggg?agcaggtagc?tgcctaatgt

361?ggtaaaggtg?gacagggccg?ggggggnaaa?ccnggaaaaa?tctactg

<210>47

<211>400

<212>DNA

<213>Homo?sapiens

<400>47

1?ctttcttttt?gcccaggctg?cagtgcaatg?gtgcgatctt?ggctcgctgc?aacctccgcc

61?tgcaacctcc?gcctcccggg?ttcaagtgat?tctcttgcct?cagcctggcg?agtagctagg

121?tttggccaaa?atatgaacta?gaaaggcagg?aacctggcca?aaggtgggga?gcttgaatgt

181?tattacctta?ttcctgcttg?acctttactg?ccaacgccaa?cacaaatggt?cagaggtccc

241?ttatatgaaa?atcttcatga?tcttgtggga?aaaccctgat?ttttgtaaag?gctgcaaaac

301?aggctcaaaa?ataaataaat?aaataaaatt?acatttaaaa?gagccagttg?cagtggctca

361?cgacgttttt?tttttttaaa?ttaaaatgtt?tcccatattt

<210>48

<211>354

<212>DNA

<213>Homo?sapiens

<400>48

1?taatttngta?atttacattt?tattctgtct?gctcanctat?tttccagttt?ttctacagng

61?tatacattat?ttaccaaatg?tatattttaa?gatattttaa?aatgttcact?ctttgattca

121?gtcattctac?ttcttagaaa?catcttaaag?naataataca?agaagtatgc?aaagatttag

181?ccacaggaat?atttatncca?aaaacgttta?cactaccatt?aaattagaaa?accaaaatgc

241?ataaagnggn?gtataaagtt?ggnctctaaa?gtcattaaaa?atntttaaca?aaatgcttac

301?agggcattct?tatgtggggg?agagaaagct?tacaaagcat?tctgtacaat?ataa

<210>49

<211>434

<212>DNA

<213>Homo?sapiens

<400>49

1?ggcttttctt?accgaattgg?ttaggattgt?cagtacaagt?gttgaattaa?ggtggtaatt

61?gtaggtaaat?gtgtttgttc?tcaatactgg?gacaaagttt?aaaatatctt?gtttttgagt

121?cttggttgaa?tttttttctt?aaattttaaa?aggatgttga?attttatgaa?gacatttgta

181?ccgcatctac?caaaatgatg?tattagttac?ctattgctgc?ataacaaatt?acctaaacat

241?tttgttggtg?aggatatgaa?gaactggggt?atcttcaaat?gctgctgggt?agggaatgtg

301?gaaatgggtg?taggccactt?tgggaaaagc?ttgtcatttc?cntcaaaatg?tttaaacata

361?cagttactgt?gttcctaggc?acttcttctc?ccggggntat?atanggcgga?aaggaaatgg

421?aaaaccattc?attt

<210>50

<211>493

<212>DNA

<213>Homo?sapiens

<400>50

1?gctatcacat?ttttntctat?tcttaagcgt?ttttctgcca?tgatcacatt?gtgatgaaga

61?acatgatggt?cactagtagg?taactttctg?tgtcattgcc?ttaatctcag?tgaggtgcta

121?gtggatttac?ctacccctgc?ttttgcatca?ccactgtaaa?tctaatagtg?aaaaggcaaa

181?tgatgtctca?gtatcactgt?gaaaacattt?ttcccttgga?ccagctgaaa?gcatcttgag

241?gagcctgaag?gcttcaaggt?ccacacgtca?aaaaaacaca?gccctaggac?tgatgggtgg

301?cccattatgg?gtgggggtca?gcttccccct?aggatcacat?ggggttctgt?tggggagggg

361?gtgggcttgt?ctgcatgacc?atcttaggtc?acctcaaggg?ggggaagggg?gcaaagggtt

421?tgcccaaggc?ntctacttgc?gtaagtagga?tagcttttaa?cccttggggn?aagggaagtt

481?ggggtttggg?tnc

Claims (3)

1, the sequence of the isolated expressed sequence tag of expressing in human liver of a class is characterized in that, comprising:

(a) sequence shown in SEQ ID No.1～SEQ ID No.50;

(b) complementary sequence of every sequence in the sequence shown in SEQ ID No.1～SEQ ID No.50;

(c) with the sequence shown in SEQ ID No.1～SEQ ID No.50 in every sequence the sequence of at least 70% homology is arranged, and

(d) one or several combination in above-mentioned (a)～(c).

2, the sequence of the isolated expressed sequence tag of expressing in human liver of a class according to claim 1 is characterized in that: described sequence comprises having the sequence shown in SEQ ID No.1～SEQ IDNo.50.

3, a kind of probe molecule is characterized in that: described probe molecule contains about 8-100 successive Nucleotide in the sequence described in the claim 1.