CN1939533A - Injection sarcosine peptide aglycone powdery injection and its making method - Google Patents
Injection sarcosine peptide aglycone powdery injection and its making method Download PDFInfo
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- CN1939533A CN1939533A CNA2006101508897A CN200610150889A CN1939533A CN 1939533 A CN1939533 A CN 1939533A CN A2006101508897 A CNA2006101508897 A CN A2006101508897A CN 200610150889 A CN200610150889 A CN 200610150889A CN 1939533 A CN1939533 A CN 1939533A
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Abstract
A powder injection in the light or dark yellow powder is prepared proportionally from polypeptide, hpyoxanthine and the excipient chosen from mannitol, sorbitol and dextran. It features that its ratio between its components varies with the chosen excipient.
Description
Technical field: the present invention relates to sarcosine peptide aglycone, be specially a kind of injection sarcosine peptide aglycone powdery injection and preparation method thereof.
Background technology: sarcosine peptide aglycone is to contain mixture such as polypeptide, aminoacid, nucleoside and nucleotide by what healthy rabbits muscle and cardiac muscle extracted, and nucleotide and several amino acids (essential amino acids) are the important substance that participates in the human life activity.Have the disturbance of blood circulation of improvement, reduction vascular resistance, increase cardiac muscle to utilize effects such as oxygen to cardiovascular system diseases, can promote the movable enhancing of hemopoietic system, white blood cell count increases, and the blood vessel elasticity of increasing is arranged simultaneously, prevent the sclerosis of blood vessels effect.Clinical Aging due to functional disorders of brain, apoplexy, the cerebral blood supply insufficiency, the peripheral nerve disease of being mainly used in.
Materials such as the polypeptide in the sarcosine peptide glycoside injection liquid, aminoacid, nucleoside and nucleotide, be vulnerable to such environmental effects in transportation, storage, sales process, particularly in the summer of China's southern area, temperature can reach 40 ℃, easily cause unstable product quality, increase incidence rate of adverse reaction.What small-volume injection used simultaneously is ampoule bottle, frangible in the production and transport process, and loss rate increases.Existing sarcosine peptide aglycone solution extraction process is not fine to polypeptide and hypoxanthic extraction effect, and yield is relatively low.Be respectively the extraction process of the sarcosine peptide aglycone solution described in 03104642.8 and 200310104970.8 the patent at application number, all relatively poor to hypoxanthic extraction effect.
Summary of the invention: the objective of the invention is to overcome the shortcoming that prior art exists, provide a kind of accent pH to tart process, thereby hypoxanthic yield is improved greatly, and don't the biological activity of polypeptides matter is affected; Increased the process of precipitate with ethanol, made the proteinic removal of impurity more thorough, injection sarcosine peptide aglycone powdery injection of improve the quality of products, constant product quality is controlled and preparation method thereof.The object of the present invention is achieved like this: it is to be the little yellow made of main component and excipient or the blocks of solid or the amorphous article of buff with polypeptide, hypoxanthine, and excipient can be mannitol, sorbitol, dextran.(1) when excipient is mannitol: every bottle contains polypeptide 3.5～17.5mg, contains hypoxanthine 0.5～2.5mg, contains excipient 30～240mg; (2) when excipient is sorbitol: every bottle contains polypeptide 3.5～17.5mg, contains hypoxanthine 0.5～2.5mg, contains excipient 30～240mg; (3) when excipient is dextran: every bottle contains polypeptide 3.5～17.5mg, contains hypoxanthine 0.5～2.5mg, contains excipient 10～200mg;
A, sarcosine peptide aglycone polypeptide solution extract:
(1) pretreatment: get fresh rabbit muscle and remove muscles and bones, fat, clean three times with purified water;
(2) rub homogenate: the rabbit muscle of handling well is rubbed with 3mm orifice plate meat grinder, add purified water, colloid mill homogenate twice, fineness 5 μ m, below-30 ℃ freezing 12～36 hours by raw material weight 1: 0.5～2.5;
(3) melt: the homogenate after the quick-freezing is put in 30～50 ℃ of water baths and is melted, freezing repeatedly thawing 2～5 times;
(4) degeneration: homogenate melts in the rearmounted jacketed pan, is heated to 80～100 ℃, behind 20～30min, cools to 30～50 ℃, filters with filter cloth, gets polypeptide extracting solution and weighing, and sampling detects content of peptides, calculated yield;
B, sarcosine peptide aglycone nucleoside solution extract:
(1) pretreatment: get fresh Cor Leporis and remove fat, connective tissue, clean three times with purified water;
(2) rub homogenate: the Cor Leporis of handling well is rubbed with 3mm orifice plate meat grinder, add 1～3 times of amount 0.14mol/LNaCl solution stirring 10～30min, colloid mill homogenate 2～3 times, fineness 5～10 μ m transfer pH value to 3.0～6.0 with glacial acetic acid;
(3) centrifugal: homogenate behind centrifugal 10～50min, is collected supernatant with 3000r/min;
(4) precipitate with ethanol: get supernatant, add ethanol, make to contain the alcohol amount and reach 60～80%, put in 0～4 ℃ of cold air static 12～36 hours.
(5) centrifugal: siphon supernatant, precipitate be with the centrifugal 15～30min of 3000r/min, repeated treatments 2～3 times, taking precipitate dissolves with 0.9%NaCl, and again with the centrifugal 15～30min of 3000r/min, getting supernatant is that nucleoside extraction solution is weighed, sampling detects hypoxanthine, calculated yield;
C, charcoal treatment: the content of extracting solution being pressed nucleoside and polypeptide is than merging, amalgamation liquid is put in the jacketed pan, add medicinal active carbon by 0.01～1% of amalgamation liquid weight, heat 80～100 ℃, stirring and adsorbing 15～30min postcooling is cooled to 30～50 ℃, filter carbon removal, reuse 0.22 μ m filtering with microporous membrane gets fine straining liquid, the calculated yield of weighing;
D, ultrafiltration: fine straining liquid is transferred pH value 6.0～8.0, and fluid temperature is controlled at 25～35 ℃, with 3000～10000 dalton's ultrafilter ultrafiltration, pressure 0.05～0.1Mpa, the sampling censorship is weighed and calculated yield, indicates name of product, batch number, quantity, refrigerated storage;
E, injection sarcosine peptide aglycone production technology:
(1) taking by weighing excipient, to add the injection water an amount of, and stirring and dissolving adds needle-use activated carbon by 0.01%～1% of solution amount, and 70～80 ℃ are stirred 20～30min, and it is clear and bright to be filtered to solution, standby;
(2) get the sarcosine peptide aglycone solution that records polypeptide and hypoxanthine content, add above-mentioned solution, transfer pH6.0～8.0, after-teeming is penetrated water to full dose;
(3) cross microporous filter membrane, behind the mensuration content, fill is in glass tube vial;
(4) sample that fill is good is inserted in the freezer dryer, is cooled to-50～-30 ℃ earlier, is incubated 1～6 hour, evacuation then, and by 1～2 ℃ of/hour intensification, after temperature reaches-5 ℃, be warming up to 35～45 ℃ by 5～10 ℃/hour, be incubated 2～4 hours, the moulding plug.Advantage of the present invention is:
(1) this product is a lyophilized formulations, and its characteristics are that quality stability is good, has avoided polypeptides matter easily polymerization to take place and influences product quality.With the sample simulation listing packing that batch is 1,2,3, under the condition of 40 ℃ of temperature, relative humidity 75%, place, respectively at sampling in the 3rd, 6,9,12,18,24 month, investigate character, acid-base value, clarity, loss on drying, content, aseptic, bacterial endotoxin, and with 0 month numeric ratio, the results are shown in Table 1.
The result shows: in 0～24 month, the character of this product is little yellow blocks of solid, clarity, aseptic, bacterial endotoxin is all up to specification, each is checked and 0 month no significant difference relatively, proves that this product is having good stability in two years.
(2) pharmacological action of this product, drug effect, usage and dosage all are equal to sarcosine peptide glycoside injection liquid, and have no side effect.
(3) the present invention improves the preparation technology of existing sarcosine peptide aglycone solution, because the hypoxanthine character that dissolubility increases in dilute acid soln, the present invention has increased accent pH to tart process in sarcosine peptide aglycone nucleoside solution extraction process, thereby hypoxanthic yield is improved greatly, and don't the biological activity of polypeptides matter is affected; The present invention has also increased the process of precipitate with ethanol in sarcosine peptide aglycone nucleoside solution extraction process, make the proteinic removal of impurity more thorough, has improved product quality.
The specific embodiment:
Embodiment 1
Get fresh rabbit muscle and remove muscles and bones, fat, clean three times with purified water.The rabbit muscle handled well is rubbed with 3mm orifice plate meat grinder, add purified water at 1: 0.5, colloid mill homogenate twice, fineness 5 μ m, below-30 ℃ freezing 12 hours by raw material weight.Homogenate after the quick-freezing is put in 30 ℃ of water baths and is melted, freezing repeatedly thawing 2 times.Homogenate melts in the rearmounted jacketed pan, is heated to 80 ℃, behind the 20min, cools to 30 ℃, filters with filter cloth, gets polypeptide extracting solution and weighing, and sampling detects content of peptides, calculated yield.Get fresh Cor Leporis and remove fat, connective tissue, clean three times with purified water.The Cor Leporis of handling well is rubbed with 3mm orifice plate meat grinder, add 1 times of amount 0.14mol/LNaCl solution stirring 10min, colloid mill homogenate 2 times, fineness 5 μ m transfer pH value to 3.0 with glacial acetic acid.Homogenate behind the centrifugal 10min, is collected supernatant with 3000r/min.Get supernatant, add ethanol, make to contain the alcohol amount and reach 60%, put in 0～4 ℃ of cold air static 12 hours.Siphon supernatant, precipitate be with the centrifugal 15min of 3000r/min, repeated treatments 2 times, and taking precipitate dissolves with 0.9%NaCl, again with the centrifugal 15min of 3000r/min, gets supernatant and is nucleoside and extract solution and weigh, and sampling detects hypoxanthine, calculated yield.The content of extracting solution being pressed nucleoside and polypeptide is than merging, amalgamation liquid is put in the jacketed pan, add medicinal active carbon by 0.01% of amalgamation liquid weight, heat 80 ℃, stirring and adsorbing 15min postcooling is cooled to 30 ℃, 0.8 the carbon removal of μ m filtering with microporous membrane, reuse 0.22 μ m filtering with microporous membrane gets fine straining liquid, the calculated yield of weighing.Fine straining liquid is transferred pH value 6.0, and fluid temperature is controlled at 25 ℃, with 3000 dalton's ultrafilter ultrafiltration, and pressure 0.05～0.1Mpa, the sampling censorship is weighed and calculated yield, indicates name of product, batch number, quantity, refrigerated storage.
Embodiment 2
Get fresh rabbit muscle and remove muscles and bones, fat, clean three times with purified water.The rabbit muscle handled well is rubbed with 3mm orifice plate meat grinder, add purified water at 1: 2.5, colloid mill homogenate twice, fineness 5 μ m, below-30 ℃ freezing 36 hours by raw material weight.Homogenate after the quick-freezing is put in 50 ℃ of water baths and is melted, freezing repeatedly thawing 5 times.Homogenate melts in the rearmounted jacketed pan, is heated to 100 ℃, behind the 30min, cools to 50 ℃, filters with filter cloth, gets polypeptide extracting solution and weighing, and sampling detects content of peptides, calculated yield.Get fresh Cor Leporis and remove fat, connective tissue, clean three times with purified water.The Cor Leporis of handling well is rubbed with 3mm orifice plate meat grinder, add 3 times of amount 0.14mol/LNaCl solution stirring 30min, colloid mill homogenate 3 times, fineness 10 μ m transfer pH value to 6.0 with glacial acetic acid.Homogenate behind the centrifugal 50min, is collected supernatant with 3000r/min.Get supernatant, add ethanol, make to contain the alcohol amount and reach 80%, put in 0～4 ℃ of cold air static 36 hours.Siphon supernatant, precipitate be with the centrifugal 30min of 3000r/min, repeated treatments 3 times, and taking precipitate dissolves with 0.9%NaCl, again with the centrifugal 30min of 3000r/min, gets supernatant and is nucleoside and extract solution and weigh, and sampling detects hypoxanthine, calculated yield.The content of extracting solution being pressed nucleoside and polypeptide is than merging, amalgamation liquid is put in the jacketed pan, add medicinal active carbon by 1% of amalgamation liquid weight, heat 100 ℃, stirring and adsorbing 30min postcooling is cooled to 50 ℃, 0.8 the carbon removal of μ m filtering with microporous membrane, reuse 0.22 μ m filtering with microporous membrane gets fine straining liquid, the calculated yield of weighing.Fine straining liquid is transferred pH value 8.0, and fluid temperature is controlled at 35 ℃, with 10000 dalton's ultrafilter ultrafiltration, and pressure 0.05～0.1Mpa, the sampling censorship is weighed and calculated yield, indicates name of product, batch number, quantity, refrigerated storage.
Embodiment 3
Above-mentioned injection sarcosine peptide aglycone production technology can for: taking by weighing mannitol 30g, to add the injection water an amount of, and stirring and dissolving adds needle-use activated carbon by 0.01% of solution amount, and 70～80 ℃ are stirred 20min, and it is clear and bright to be filtered to solution, standby.Get the sarcosine peptide aglycone solution of recipe quantity, add above-mentioned mannitol solution, transfer pH6.0, after-teeming is penetrated water to 1000ml.Cross 0.22 μ m microporous filter membrane, behind the mensuration content, fill is in glass tube vial.The sample that fill is good is inserted in the freezer dryer, is cooled to-50 ℃ earlier, is incubated 1 hour, evacuation then, and, after temperature reaches-5 ℃, be warming up to 45 ℃ by 5 ℃/hour by 2 ℃ of/hour intensifications, be incubated 2 hours, the moulding plug.Zha Gai, quality inspection, vanning.
Embodiment 4
Above-mentioned injection sarcosine peptide aglycone production technology can for: taking by weighing mannitol 240g, to add the injection water an amount of, and stirring and dissolving adds needle-use activated carbon by 1% of solution amount, and 70～80 ℃ are stirred 30min, and it is clear and bright to be filtered to solution, standby.Get the sarcosine peptide aglycone solution of recipe quantity, add above-mentioned mannitol solution, transfer pH8.0, after-teeming is penetrated water to 2400ml.Cross 0.22 μ m microporous filter membrane, behind the mensuration content, fill is in glass tube vial.The sample that fill is good is inserted in the freezer dryer, is cooled to-30 ℃ earlier, is incubated 6 hours, evacuation then, and, after temperature reaches-5 ℃, be warming up to 35 ℃ by 10 ℃/hour by 2 ℃ of/hour intensifications, be incubated 4 hours, the moulding plug.Zha Gai, quality inspection, vanning.
Embodiment 5
Above-mentioned injection sarcosine peptide aglycone production technology can for: taking by weighing sorbitol 30g, to add the injection water an amount of, and stirring and dissolving adds needle-use activated carbon by 0.01% of solution amount, and 70～80 ℃ are stirred 20min, and it is clear and bright to be filtered to solution, standby.Get the sarcosine peptide aglycone solution of recipe quantity, add above-mentioned sorbitol solution, transfer pH6.0, after-teeming is penetrated water to 1000ml.Cross 0.22 μ m microporous filter membrane, behind the mensuration content, fill is in glass tube vial.The sample that fill is good is inserted in the freezer dryer, is cooled to-50 ℃ earlier, is incubated 1 hour, evacuation then, and, after temperature reaches-5 ℃, be warming up to 45 ℃ by 5 ℃/hour by 2 ℃ of/hour intensifications, be incubated 2 hours, the moulding plug.Zha Gai, quality inspection, vanning.
Embodiment 6
Above-mentioned injection sarcosine peptide aglycone production technology can for: taking by weighing sorbitol 240g, to add the injection water an amount of, and stirring and dissolving adds needle-use activated carbon by 1% of solution amount, and 70～80 ℃ are stirred 30min, and it is clear and bright to be filtered to solution, standby.Get the sarcosine peptide aglycone solution of recipe quantity, add above-mentioned sorbitol solution, transfer pH8.0, after-teeming is penetrated water to 2400ml.Cross 0.22 μ m microporous filter membrane, behind the mensuration content, fill is in glass tube vial.The sample that fill is good is inserted in the freezer dryer, is cooled to-30 ℃ earlier, is incubated 6 hours, evacuation then, and, after temperature reaches-5 ℃, be warming up to 35 ℃ by 10 ℃/hour by 2 ℃ of/hour intensifications, be incubated 4 hours, the moulding plug.Zha Gai, quality inspection, vanning.
Embodiment 7
Above-mentioned injection sarcosine peptide aglycone production technology can for: taking by weighing Dextran 10 g, to add the injection water an amount of, and stirring and dissolving adds needle-use activated carbon by 0.01% of solution amount, and 70～80 ℃ are stirred 20min, and it is clear and bright to be filtered to solution, standby.Get the sarcosine peptide aglycone solution of recipe quantity, add above-mentioned dextran solution, transfer pH6.0, after-teeming is penetrated water to 1000ml.Cross 0.22 μ m microporous filter membrane, behind the mensuration content, fill is in glass tube vial.The sample that fill is good is inserted in the freezer dryer, is cooled to-50 ℃ earlier, is incubated 1 hour, evacuation then, and, after temperature reaches-5 ℃, be warming up to 45 ℃ by 5 ℃/hour by 2 ℃ of/hour intensifications, be incubated 2 hours, the moulding plug.Zha Gai, quality inspection, vanning.
Embodiment 8
Above-mentioned injection sarcosine peptide aglycone production technology can for: taking by weighing Dextran-20 0g, to add the injection water an amount of, and stirring and dissolving adds needle-use activated carbon by 1% of solution amount, and 70～80 ℃ are stirred 30min, and it is clear and bright to be filtered to solution, standby.Get the sarcosine peptide aglycone solution of recipe quantity, add above-mentioned dextran solution, transfer pH8.0, after-teeming is penetrated water to 2400ml.Cross 0.22 μ m microporous filter membrane, behind the mensuration content, fill is in glass tube vial.The sample that fill is good is inserted in the freezer dryer, is cooled to-30 ℃ earlier, is incubated 6 hours, evacuation then, and, after temperature reaches-5 ℃, be warming up to 35 ℃ by 10 ℃/hour by 2 ℃ of/hour intensifications, be incubated 4 hours, the moulding plug.Zha Gai, quality inspection, vanning.
Table 1, injection sarcosine peptide aglycone study on the stability
| Month | 0 | 3 | 6 | 9 | 12 | 18 | 24 | ||||||||||||||
| Batch | 1 | 2 | 3 | 1 | 2 | 3 | 1 | 2 | 3 | 1 | 2 | 3 | 1 | 2 | 3 | 1 | 2 | 3 | 1 | 2 | 3 |
| Acid-base value loss on drying (%) content of peptides (%) hypoxanthine content (%) | 7.43 0.49 99.8 99.7 | 7.51 0.48 99.8 99.8 | 7.48 0.63 99.7 99.7 | 7.44 0.33 99.6 99.5 | 7.48 0.42 99.7 99.8 | 7.49 0.51 99.7 99.8 | 7.42 0.51 99.3 99.3 | 7.50 0.41 99.5 99.5 | 7.43 0.42 99.6 99.7 | 7.40 0.58 99.6 99.6 | 7.50 0.53 99.5 99.3 | 7.46 0.44 99.4 99.5 | 7.41 0.49 99.5 99.5 | 7.47 0.56 99.3 99.4 | 7.45 0.42 99.3 99.4 | 7.38 0.52 99.2 99.6 | 7.46 0.55 99.3 99.3 | 7.43 0.58 99.2 99.2 | 7.40 0.50 99.3 99.4 | 7.47 0.51 99.1 99.0 | 7.45 0.54 99.3 99.2 |
Claims (1)
1, a kind of injection sarcosine peptide aglycone powdery injection and preparation method thereof, it is the little yellow made of main component and excipient or the blocks of solid or the amorphous article of buff by polypeptide, hypoxanthine, excipient can be mannitol, sorbitol, dextran, it is characterized in that: when (1) is mannitol when excipient: every bottle contains polypeptide 3.5～17.5mg, contain hypoxanthine 0.5～2.5mg, contain excipient 30～240mg; (2) when excipient is sorbitol: every bottle contains polypeptide 3.5～17.5mg, contains hypoxanthine 0.5～2.5mg, contains excipient 30～240mg; (3) when excipient is dextran: every bottle contains polypeptide 3.5～17.5mg, contains hypoxanthine 0.5～2.5mg, contains excipient 10～200mg;
A, sarcosine peptide aglycone polypeptide solution extract:
(1) pretreatment: get fresh rabbit muscle and remove muscles and bones, fat, clean three times with purified water;
(2) rub homogenate: the rabbit muscle of handling well is rubbed with 3mm orifice plate meat grinder, add purified water, colloid mill homogenate twice, fineness 5 μ m, below-30 ℃ freezing 12～36 hours by raw material weight 1: 0.5～2.5;
(3) melt: the homogenate after the quick-freezing is put in 30～50 ℃ of water baths and is melted, freezing repeatedly thawing 2～5 times;
(4) degeneration: homogenate melts in the rearmounted jacketed pan, is heated to 80～100 ℃, behind 20～30min, cools to 30～50 ℃, filters with filter cloth, gets polypeptide extracting solution and weighing, and sampling detects content of peptides, calculated yield;
B, sarcosine peptide aglycone nucleoside solution extract:
(1) pretreatment: get fresh Cor Leporis and remove fat, connective tissue, clean three times with purified water;
(2) rub homogenate: the Cor Leporis of handling well is rubbed with 3mm orifice plate meat grinder, add 1～3 times of amount 0.14mol/LNaCl solution stirring 10～30min, colloid mill homogenate 2～3 times, fineness 5～10 μ m transfer pH value to 3.0～6.0 with glacial acetic acid;
(3) centrifugal: homogenate behind centrifugal 10～50min, is collected supernatant with 3000r/min;
(4) precipitate with ethanol: get supernatant, add ethanol, make to contain the alcohol amount and reach 60～80%, put in 0～4 ℃ of cold air static 12～36 hours;
(5) centrifugal: siphon supernatant, precipitate be with the centrifugal 15～30min of 3000r/min, repeated treatments 2～3 times, taking precipitate dissolves with 0.9%NaCl, and again with the centrifugal 15～30min of 3000r/min, getting supernatant is that nucleoside extraction solution is weighed, sampling detects hypoxanthine, calculated yield;
C, charcoal treatment: the content of extracting solution being pressed nucleoside and polypeptide is than merging, amalgamation liquid is put in the jacketed pan, add medicinal active carbon by 0.01～1% of amalgamation liquid weight, heat 80～100 ℃, stirring and adsorbing 15～30min postcooling is cooled to 30～50 ℃, filter carbon removal, reuse 0.22 μ m filtering with microporous membrane gets fine straining liquid, the calculated yield of weighing;
D, ultrafiltration: fine straining liquid is transferred pH value 6.0～8.0, and fluid temperature is controlled at 25～35 ℃, with 3000～10000 dalton's ultrafilter ultrafiltration, pressure 0.05～0.1Mpa, the sampling censorship is weighed and calculated yield, indicates name of product, batch number, quantity, refrigerated storage;
E, injection sarcosine peptide aglycone production technology:
(1) taking by weighing excipient, to add the injection water an amount of, and stirring and dissolving adds needle-use activated carbon by 0.01%～1% of solution amount, and 70～80 ℃ are stirred 20～30min, and it is clear and bright to be filtered to solution, standby;
(2) get the sarcosine peptide aglycone solution that records polypeptide and hypoxanthine content, add above-mentioned solution, transfer pH 6.0～8.0, after-teeming is penetrated water to full dose;
(3) cross microporous filter membrane, behind the mensuration content, fill is in glass tube vial;
(4) sample that fill is good is inserted in the freezer dryer, is cooled to-30～-50 ℃ earlier, is incubated 1～6 hour, evacuation then, and by 1～2 ℃ of/hour intensification, after temperature reaches-5 ℃, be warming up to 35～45 ℃ by 5～10 ℃/hour, be incubated 2～4 hours, the moulding plug.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101584713A (en) * | 2009-07-03 | 2009-11-25 | 石海 | Muscular amino acids and peptides and nucleosides injection and preparing method thereof |
| CN102697810A (en) * | 2012-06-19 | 2012-10-03 | 夏智红 | MAAPN extractive and composition thereof |
| CN102727428A (en) * | 2011-04-12 | 2012-10-17 | 河南科伦药业有限公司 | Preparation method of muscular amino acids and nucleosides injection |
| CN103040868A (en) * | 2012-12-17 | 2013-04-17 | 海南百思特医药科技有限公司 | Muscular amino acid and polypeptide and nucleoside liposome injection |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1233414C (en) * | 2003-02-19 | 2005-12-28 | 江卫世 | Muscular amino acid and peptide nucleoside powder injection and its preparation method |
| CN1611261A (en) * | 2003-10-30 | 2005-05-04 | 王玫 | Medicinal preparation of muscular amino acids and nucleosides for injection and its preparing method |
-
2006
- 2006-10-13 CN CN2006101508897A patent/CN1939533B/en active Active
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101584713A (en) * | 2009-07-03 | 2009-11-25 | 石海 | Muscular amino acids and peptides and nucleosides injection and preparing method thereof |
| CN102727428A (en) * | 2011-04-12 | 2012-10-17 | 河南科伦药业有限公司 | Preparation method of muscular amino acids and nucleosides injection |
| CN102727428B (en) * | 2011-04-12 | 2014-12-31 | 河南科伦药业有限公司 | Preparation method of muscular amino acids and nucleosides injection |
| CN102697810A (en) * | 2012-06-19 | 2012-10-03 | 夏智红 | MAAPN extractive and composition thereof |
| CN103040868A (en) * | 2012-12-17 | 2013-04-17 | 海南百思特医药科技有限公司 | Muscular amino acid and polypeptide and nucleoside liposome injection |
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| CN1939533B (en) | 2010-09-08 |
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