CN1935102B - Experimental animal grouping method - Google Patents

Abstract

The present invention discloses a method for grouping experimental animals. Said method includes the following steps: (1), determining marker value of every individuality in the animal samples to be grouped; (2), according to the group number required fro animal experiment, and individuality number of every group and marker values of all the individuatilies making grouping operation. Besides, saidinvention also provides its grouping formula, and provides its operation method.

Description

A kind of method of laboratory animal grouping

Technical field

The present invention relates to a kind of method of laboratory animal grouping.

Background technology

In the medical research, how the design that must experimentize before specifically testing is for example divided into groups, how rationally to be estimated sample example number in each processed group (treatment combination), how further operation is done in the aspects such as control of non-processing factor and arrange study subject.Contrast, be the big principle of three in the experimental design with repeating at random, this is that any experiment all should hig diligence and follow.

Contrast is the First Principles of experimental design.Have comparison to differentiate, contrast is the basis of comparison.Except being observed the processing factor, other tangent condition that influences effect index should be tried one's best together neat in experimental group and matched group, and the comparability of height be arranged, and could get rid of the influence of Confounding Factor, and the project of experimental observation is made scientific conclusion.The kind of contrast has a lot, can be selected according to research purpose and content.Commonly used having is following several: 1. blank, matched group does not apply any processing factor.This method is simple, but causes experimental group and matched group difference psychologically easily, thereby influences the mensuration of experimental effect.Clinical observation on the therapeutic effect generally should not adopt this kind contrast.2. placebo, matched group adopts a kind of placebo that does not have pharmacological action, pharmaceutical dosage form or dispose and can not discern for study subject.Because spiritual psychological factor also can produce material impact with the endocrine multipath to body and disease by neural.Clinical efficacy about 30% is from the psychological effect of patient to medical personnel and medical measure according to estimates.But must note in clinical research, must following patient's interests first principle.It is generally acknowledged the chronic disease that only has or not specific treatment, can use placebo.3. experiment condition contrast, matched group does not apply the processing factor, but applies certain experiment condition identical with the processing factor.Experiment condition comprises the solvent of operating technology, tested factor or capacity etc.All experiment conditions that test effect is exerted an influence should adopt this method.4. standard control is done contrast with existing standard method or conventional method.When observe estimating certain medicine or therapy, for not incuring loss through delay patient's treatment, for acute disease, critical illness with the disease of special treatment way is arranged, all use known active drug, effectively therapy or the recognized standard therapy compare to certain sick curative effect.

Clinical efficacy judges that it is not enough that self cross-reference is only arranged, because the generation of numerous disease, development are relevant with factors such as time, seasons.Secondly, for most diseases, historical control also is accessory, because along with the transition in age, significant change has taken place many conditions; But the disease of " incurable disease " thought at present can also be historical as potential contrast.

In experimentation, not only require to have contrast, except the processing factor, other may produce the non-processing factor (contrast and experimental group) in each group that mixes effect and be consistent as far as possible, keep the harmony of respectively organizing between also requiring respectively to organize.Implement randomization and be the balanced important means between the raising group; When also being data statistics analyse, carry out the prerequisite of statistical inference.The purpose of randomized sampling to make exactly overall in each object of study all have equal chance to be extracted to be assigned to experimental group or matched group.

Make statistic (sample index) representation parameter (overall objective), except that dwindling the error with arbitrary sampling method, repeated experiments is to guarantee experimental result another basic skills reliably, and this is another basic principle of experimental design.The certain repeat number of requirement of experiment its objective is that to make mean true to nature, and stability criterion is poor, only in this way could represent overall parameter from the statistic of sample, and statistical inference just has reliable premise.

The determiner that repeats routine number (sample example number) comprises the size, data character of size, the bion variation of the explicitly of the effect of processing, experimental error, definite error of first kind (α) and the size of error of the second kind (β) and type of experimental design.In a word, sample example number all is unfavorable for disclosing the difference between things too much or very little.For this reason, should guarantee that experimental result has under the condition of certain reliability, determine minimum sample example number, so that save manpower and funds.

In drug research, usually need to carry out zoopery.Must relate to the animal grouping in the zoopery, as in drug toxicity research, the animal toxicity of research different dosing dosage group (matched group, high dose group, middle dosage group, low dose group), and research medicine breeding toxicity; In drug metabolism research, linear pharmacokinetic study needs three various dose groups and matched group at least; Learn in the research at medicine effect, main pharmacodynamics research should be established matched group, comprises blank (normally reach the animal pattern matched group, should establish the solvent matched group in case of necessity) and positive drug matched group, and experimental group also needs a plurality of various dose and different modes of administration.

When carrying out zoopery, often the laboratory animal that chooses need be divided into some groups by the needs of studying.The animal grouping should be by the principle of random assortment, make every animal all have equal chance to be assigned in each experimental group and the matched group and go,, influence experimental result to avoid the difference between each group, particularly carry out statistical test accurately, must on the basis of random packet, carry out.Every treated animal quantity should be by experimental period length, experiment type and statistics requirement and is decided.If chronic experiment or need regularly to put to death the experiment that animal is tested just requires to select more animal, to supply the animal natural death and to think and put to death the quantity of being lost, guaranteeing to test has the size of animal existence that conforms with the statistics requirement when finishing.

Evenly grouping is significant to the animal grouping, and evenly grouping can make every group sample attribute unanimity, and group difference can not influence experimental result; And traditional method is used random packet, does not reach the optimization of even grouping, and group difference will cause the experimental result difference, influences the correct analysis of researcher to result of study.

Summary of the invention

The method that the purpose of this invention is to provide a kind of laboratory animal grouping.

The method of laboratory animal grouping provided by the present invention may further comprise the steps:

1) measures each individual value of statistical indicant in the animal specimen of waiting to divide into groups;

2) number of individuals of the group number that requires according to zoopery and every group is to 1) in all individual values of statistical indicant divide into groups, find out

The sort of value of statistical indicant grouping situation of the value minimum of (average variance between group); Wherein n is the group number of requirement of experiment, Be every group value of statistical indicant average,

Value of statistical indicant average for all samples;

3) according to

The sort of value of statistical indicant grouping situation of value minimum laboratory animal is divided into groups.

The method of this laboratory animal grouping can be used for the drug toxicity research experiment, and drug metabolism research experiment, medicine effect are learned the animal grouping in the research experiment.

Described sign is for the title that illustrates that individual feature in one aspect adopts, as body weight.Its sign performance available numeric representation, i.e. value of statistical indicant.As body weight is 300 grams.

Described sign can be selected from any one in body weight, blood pressure and the body surface area etc.

The present invention according to

The minimum method of value averages grouping to zoopery, makes the group difference minimum of value of statistical indicant, could guarantee the collimation of data between matched group and the different experiments group like this, and is also minimum to the influence of experimental result.

Description of drawings

Fig. 1 is a main flow chart of obtaining the calculation procedure of the sort of value of statistical indicant grouping situation of mean square difference minimum between group

The specific embodiment

The present invention utilizes the Monte Carlo principle, uses computer program, by COMPUTER CALCULATION, tries to achieve optimum animal grouping (promptly The sort of value of statistical indicant grouping situation that value is minimum), it is more excellent to reach grouping, guarantees that group difference is littler.

Wherein, the Monte Carlo principle is exemplified below:

The value of statistical indicant of 200 mices is divided into 4 groups at random, calculates the meansigma methods of every group mark value, try to achieve average variance between the group of value of statistical indicant.Random packet is 10E+6 time like this, tries to achieve average variance between each group, gets the random sample of average variance minimum between group and divides into groups as animal.

200 animals are divided into four groups sample size at random: C;

The probability that optimum grouping occurs is 1/C, i.e. 0.000...0001;

The probability that optimum grouping does not have to occur is 1-1/C, i.e. 0.999...999;

The probability that random packet 10E+6 time, optimum grouping once do not have to occur yet is the 10E+6 power of (1-1/C), and this value levels off to zero;

The probability that optimum grouping drops on random packet 10E+6 sample size levels off to 1;

Obtain optimum grouping in the random packet 10E+6 sample size, promptly

The sort of value of statistical indicant grouping situation that value is minimum.

Obtain The main flow chart of the calculation procedure of the sort of value of statistical indicant grouping situation that value is minimum is as shown in Figure 1:

Program begins to get access to animal information to be divided into groups (as numbering, body weight) and group number that will divide and every group size of animal.

Initialization one number is as keeping variance yields then, and this number will guarantee to find than average variance between the little group of this number more greatly; And a repetition factor is set, and to start from scratch, in fact this is exactly a cycle counter.

Then all animal values of statistical indicant are carried out random packet according to the packet mode that gets access to previously, calculate average variance between new group.

Judge that whether average variance is less than keeping variance yields between new group, if less than keeping variance yields, just replace the reservation variance yields with average variance between new group, enter next step then: repetition factor is from adding 1.If average variance is not less than the reservation variance yields between new group, just directly enter next step: repetition factor is from adding 1.

Repetition factor is from adding 1, check whether repetition factor has arrived the number of times that needs, being divided into four groups at random as 200 animals need circulate 10E+6 time, to just the grouping information of last reservation being exported out, just be less than and carry out the random packet of front again, average variance between calculating group again judges that whether average variance up to repetition factor has reached the number of times that need less than keeping variance yields between new group.

Experimental technique among the following embodiment if no special instructions, is conventional method.

Be example only below, animal group technology of the present invention be described with the body weight, the animal group technology of other sign with the body weight be the sign identical.

1,220 rats of embodiment are divided into four groups, 50 every group

1,220 rats raising are measured body weight, the result is as shown in table 1:

The body weight of table 1,220 rats

Body weight (g)	Numbering	Body weight (g)	Numbering	Body weight (g)	Numbering	Body weight (g)	Numbering
								280.12	1001	295.36	1056	310.52	1111	285.89	1166
281.12	1002	296.36	1057	311.52	1112	286.89	1167
								282.12	1003	297.36	1058	312.52	1113	287.89	1168

283.12	1004	298.36	1059	313.52	1114	288.89	1169
								284.12	1005	299.36	1060	314.52	1115	289.89	1170
285.12	1006	300.36	1061	315.52	1116	290.89	1171
								286.12	1007	301.36	1062	316.52	1117	291.89	1172
287.12	1008	302.36	1063	317.52	1118	292.89	1173
								288.12	1009	303.36	1064	318.52	1119	293.89	1174
289.12	1010	304.36	1065	319.52	1120	294.89	1175
								290.12	1011	305.36	1066	280.46	1121	295.89	1176
291.12	1012	306.36	1067	281.46	1122	296.89	1177
								292.12	1013	307.36	1068	282.46	1123	297.89	1178
293.12	1014	308.36	1069	283.46	1124	298.89	1179
								294.12	1015	309.36	1070	284.46	1125	299.89	1180
295.12	1016	310.36	1071	285.46	1126	300.89	1181

283.12	1004	298.36	1059	313.52	1114	288.89	1169
								296.12	1017	311.36	1072	286.46	1127	301.89	1182
297.12	1018	312.36	1073	287.46	1128	302.89	1183
								298.12	1019	313.36	1074	288.46	1129	303.89	1184
299.12	1020	314.36	1075	289.46	1130	304.89	1185
								300.12	1021	315.36	1076	290.46	1131	305.89	1186
301.12	1022	316.36	1077	291.46	1132	306.89	1187
								302.12	1023	317.36	1078	292.46	1133	307.89	1188
303.12	1024	318.36	1079	293.46	1134	308.89	1189
								304.12	1025	319.36	1080	294.46	1135	309.89	1190
305.12	1026	280.52	1081	295.46	1136	310.89	1191
								306.12	1027	281.52	1082	296.46	1137	311.89	1192
307.12	1028	282.52	1083	297.46	1138	312.89	1193
								308.12	1029	283.52	1084	298.46	1139	313.89	1194
309.12	1030	284.52	1085	299.46	1140	314.89	1195
								310.12	1031	285.52	1086	300.46	1141	315.89	1196
311.12	1032	286.52	1087	301.46	1142	316.89	1197
								312.12	1033	287.52	1088	302.46	1143	317.89	1198
313.12	1034	288.52	1089	303.46	1144	318.89	1199
								314.12	1035	289.52	1090	304.46	1145	319.89	1200
315.12	1036	290.52	1091	305.46	1146	296.11	1201
								316.12	1037	291.52	1092	306.46	1147	297.11	1202
317.12	1038	292.52	1093	307.46	1148	298.11	1203

283.12	1004	298.36	1059	313.52	1114	288.89	1169
								318.12	1039	293.52	1094	308.46	1149	299.11	1204
319.12	1040	294.52	1095	309.46	1150	300.11	1205
								280.36	1041	295.52	1096	310.46	1151	301.11	1206

281.36	1042	296.52	1097	311.46	1152	302.11	1207
								282.36	1043	297.52	1098	312.46	1153	303.11	1208
283.36	1044	298.52	1099	313.46	1154	304.11	1209
								284.36	1045	299.52	1100	314.46	1155	305.11	1210
285.36	1046	300.52	1101	315.46	1156	306.11	1211
								286.36	1047	301.52	1102	316.46	1157	307.11	1212
287.36	1048	302.52	1103	317.46	1158	308.11	1213
								288.36	1049	303.52	1104	318.46	1159	309.11	1214
289.36	1050	304.52	1105	319.46	1160	310.11	1215
								290.36	1051	305.52	1106	280.89	1161	311.11	1216
291.36	1052	306.52	1107	281.89	1162	312.11	1217
								292.36	1053	307.52	1108	282.89	1163	313.11	1218
293.36	1054	308.52	1109	283.89	1164	314.11	1219
								294.36	1055	309.52	1110	284.89	1165	315.11	1220

2, according to the weight of animals, with population mean be foundation apart from distance, leave out the beginning and the end, remove body weight the higher person and junior, take out 200;

3, use random sampling method and method of the present invention to divide into groups respectively, be divided into four groups, 50 every group, calculate every group weight average value, sample the weight average value,

Value.

Use the group result of random sampling method as shown in table 2, average variance between its group

Be 1.553783.

According to method of the present invention, with the body weight value of 200 rats, the input computer draws by aforementioned calculation machine program The sort of body weight value grouping situation of value minimum, according to this body weight value grouping situation 200 rats divide into groupss, the result is as shown in table 3, average variance between it is organized Be 3.99E-05.Illustrate that method of the present invention compares with random sampling method, its variance is much smaller, and is also minimum to the influence of experimental result.

N=4 wherein,

Be every group weight average number, Weight average number for population sample.

The random packet of table 2.200 rat

First group		Second group		The 3rd group		The 4th group
								Numbering	Body weight (g)	Numbering	Body weight (g)	Numbering	Body weight (g)	Numbering	Body weight (g)
1032	311.12	1214	309.11	1051	290.36	1017	296.12

1069	308.36	1117	316.52	1099	298.52	1022	301.12
								1206	301.11	1059	298.36	1213	308.11	1034	313.12
1194	313.89	1142	301.46	1182	301.89	1124	283.46
								1108	307.52	1131	290.46	1050	289.36	1013	292.12
1102	301.52	1217	312.11	1077	316.36	1075	314.36
								1071	310.36	1177	296.89	1205	300.11	1141	300.46
1003	282.12	1025	304.12	1012	291.12	1193	312.89
								1186	305.89	1106	305.52	1174	293.89	1127	286.46
1146	305.46	1030	309.12	1076	315.36	1073	312.36
								1138	297.46	1183	302.89	1191	310.89	1083	282.52
1168	287.89	1090	289.52	1155	314.46	1014	293.12
								1196	315.89	1029	308.12	1047	286.36	1166	285.89
1007	286.12	1094	293.52	1126	285.46	1136	295.46

1069	308.36	1117	316.52	1099	298.52	1022	301.12
								1016	295.12	1046	285.36	1074	313.36	1008	287.12
1144	303.46	1189	308.89	1095	294.52	1060	299.36
								1172	291.89	1151	310.46	1137	296.46	1123	282.46
1103	302.52	1088	287.52	1043	282.36	1158	317.46
								1015	294.12	1078	317.36	1207	302.11	1209	304.11
1097	296.52	1068	307.36	1033	312.12	1116	315.52
								1152	311.46	1006	285.12	1056	295.36	1153	312.46
1105	304.52	1011	290.12	1045	284.36	1020	299.12
								1208	303.11	1100	299.52	1107	306.52	1054	293.36
1140	299.46	1210	305.11	1179	298.89	1173	292.89
								1110	309.52	1066	305.36	1084	283.52	1216	311.11
1188	307.89	1148	307.46	1171	290.89	1089	288.52
								1091	290.52	1162	281.89	1220	315.11	1052	291.36
1195	314.89	1215	310.11	1093	292.52	1176	295.89
								1167	286.89	1092	291.52	1219	314.11	1118	317.52
1218	313.11	1024	303.12	1004	283.12	1037	316.12
								1009	288.12	1031	310.12	1164	283.89	1028	307.12
1096	295.52	1067	306.36	1192	311.89	1170	289.89
								1058	297.36	1202	297.11	1204	299.11	1057	296.36
1026	305.12	1109	308.52	1005	284.12	1021	300.12
								1128	287.46	1048	287.36	1027	306.12	1062	301.36
1049	288.36	1019	298.12	1087	286.52	1203	298.11

1069	308.36	1117	316.52	1099	298.52	1022	301.12
								1055	294.36	1023	302.12	1178	297.89	1070	309.36
1154	313.46	1180	299.89	1086	285.52	1185	304.89
								1035	314.12	1190	309.89	1211	306.11	1145	304.46
1163	282.89	1085	284.52	1149	308.46	1184	303.89
								1133	292.46	1072	311.36	1063	302.36	1104	303.52
1143	302.46	1114	313.52	1112	311.52	1156	315.46
								1125	284.46	1115	314.52	1101	300.52	1139	298.46
1201	296.11	1129	288.46	1165	284.89	1181	300.89

1111	310.52	1113	312.52	1010	289.12	1169	288.89
								1036	315.12	1132	291.46	1157	316.46	1061	300.36
1064	303.36	1098	297.52	1130	289.46	1212	307.11
								1197	316.89	1044	283.36	1135	294.46	1038	317.12
1150	309.46	1065	304.36	1134	293.46	1018	297.12
								1187	306.89	1147	306.46	1053	292.36	1175	294.89
This class mean	301.2842	This class mean	301.231	This class mean	298.2364	This class mean	300.6644
								Sample mean is:		300.354		Average variance is between group:		1.553783

Table 3,200 rats divide into groups according to method of the present invention

First group		Second group		The 3rd group		The 4th group
								Numbering	Body weight (g)	Numbering	Body weight (g)	Numbering	Body weight (g)	Numbering	Body weight (g)
1024	303.12	1217	312.11	1170	289.89	1202	297.11

First group		Second group		The 3rd group		The 4th group
								1021	300.12	1183	302.89	1130	289.46	1053	292.36
1006	285.12	1192	311.89	1166	285.89	1169	288.89
								1176	295.89	1129	288.46	1049	288.36	1164	283.89
1057	296.36	1007	286.12	1047	286.36	1069	308.36
								1003	282.12	1205	300.11	1055	294.36	1210	305.11
1105	304.52	1123	282.46	1132	291.46	1075	314.36
								1004	283.12	1182	301.89	1092	291.52	1013	292.12
1140	299.46	1168	287.89	1077	316.36	1091	290.52
								1064	303.36	1048	287.36	1118	317.52	1193	312.89
1005	284.12	1186	305.89	1167	286.89	1052	291.36
								1037	316.12	1219	314.11	1145	304.46	1050	289.36
1115	314.52	1009	288.12	1076	315.36	1027	306.12
								1035	314.12	1154	313.46	1215	310.11	1090	289.52
1094	293.52	1088	287.52	1028	307.12	1065	304.36
								1204	299.11	1016	295.12	1151	310.46	1134	293.46
1101	300.52	1098	297.52	1128	287.46	1188	307.89
								1125	284.46	1191	310.89	1046	285.36	1074	313.36
1114	313.52	1083	282.52	1051	290.36	1190	309.89
								1102	301.52	1043	282.36	1144	303.46	1179	298.89
1058	297.36	1196	315.89	1063	302.36	1112	311.52
								1010	289.12	1109	308.52	1108	307.52	1187	306.89
1029	308.12	1060	299.36	1044	283.36	1054	293.36

First group		Second group		The 3rd group		The 4th group
								1073	312.36	1207	302.11	1131	290.46	1148	307.46
1177	296.89	1178	297.89	1218	313.11	1213	308.11
								1019	298.12	1025	304.12	1149	308.46	1008	287.12
1174	293.89	1150	309.46	1011	290.12	1099	298.52
								1143	302.46	1220	315.11	1012	291.12	1214	309.11
1138	297.46	1084	283.52	1175	294.89	1036	315.12
								1152	311.46	1106	305.52	1015	294.12	1093	292.52

1038	317.12	1197	316.89	1100	299.52	1068	307.36
								1104	303.52	1184	303.89	1208	303.11	1147	306.46
1181	300.89	1185	304.89	1195	314.89	1095	294.52
								1172	291.89	1124	283.46	1110	309.52	1086	285.52
1032	311.12	1212	307.11	1216	311.11	1156	315.46
								1206	301.11	1157	316.46	1111	310.52	1153	312.46
1031	310.12	1158	317.46	1194	313.89	1146	305.46
								1097	296.52	1163	282.89	1201	296.11	1089	288.52
1085	284.52	1136	295.46	1133	292.46	1070	309.36
								1017	296.12	1026	305.12	1117	316.52	1103	302.52
1137	296.46	1014	293.12	1033	312.12	1059	298.36
								1142	301.46	1135	294.46	1113	312.52	1209	304.11
1171	290.89	1023	302.12	1022	301.12	1141	300.46
								1116	315.52	1034	313.12	1203	298.11	1139	298.46
1062	301.36	1018	297.12	1020	299.12	1162	281.89

1038	317.12	1197	316.89	1100	299.52	1068	307.36
								1061	300.36	1071	310.36	1127	286.46	1173	292.89
1189	308.89	1045	284.36	1072	311.36	1155	314.46
								1107	306.52	1126	285.46	1030	309.12	1165	284.89
1211	306.11	1078	317.36	1066	305.36	1056	295.36
								1096	295.52	1067	306.36	1087	286.52	1180	299.89
This class mean	300.3596	This class mean	300.3532	This class mean	300.3439	This class mean	300.3592
								Sample mean is:		300.354		Average variance is between group:		3.99E-05

Embodiment 2, get 150 from 170 animals, be divided into three groups, each treated animal number is respectively: 40,50,60

1,170 rats raising are measured body weight, the result is as shown in table 4:

The body weight of table 4,170 rats

Body weight (g)	Numbering	Body weight (g)	Numbering	Body weight (g)	Numbering	Body weight (g)	Numbering
								280.14	L001	303.37	L044	312.95	L087	292.26	L129
281.14	L002	304.37	L045	313.95	L088	293.26	L130
								282.14	L003	305.37	L046	314.95	L089	294.26	L131
283.14	L004	306.37	L047	315.95	L090	295.26	L132
								284.14	L005	307.37	L048	292.23	L091	296.26	L133
285.14	L006	308.37	L049	293.23	L092	297.26	L134
								286.14	L007	309.37	L050	294.23	L093	298.26	L135
287.14	L008	310.37	L051	295.23	L094	299.26	L136
								288.14	L009	311.37	L052	296.23	L095	300.26	L137
289.14	L010	312.37	L053	297.23	L096	301.26	L138
								290.14	L011	313.37	L054	298.23	L097	302.26	L139

291.14	L012	314.37	L055	299.23	L098	303.26	L140
								292.14	L013	315.37	L056	300.23	L099	304.26	L141
293.14	L014	316.37	L057	301.23	L100	305.26	L142
								294.14	L015	317.37	L058	302.23	L101	306.26	L143
295.14	L016	318.37	L059	303.23	L102	307.26	L144
								296.14	L017	319.37	L060	304.23	L103	308.26	L145
297.14	L018	286.95	L061	305.23	L104	309.26	L146
								298.14	L019	287.95	L062	306.23	L105	310.26	L147
299.14	L020	288.95	L063	307.23	L106	311.26	L148
								300.14	L021	289.95	L064	308.23	L107	312.26	L149
301.14	L022	290.95	L065	309.23	L108	313.26	L150
								302.14	L023	291.95	L066	310.23	L109	291.26	L151
303.14	L024	292.95	L067	311.23	L110	292.26	L152
								304.14	L025	293.95	L068	312.23	L111	293.26	L153
305.14	L026	294.95	L069	313.23	L112	294.26	L154
								306.14	L027	295.95	L070	314.23	L113	295.26	L155
307.14	L028	296.95	L071	315.23	L114	296.26	L156
								308.14	L029	297.95	L072	316.23	L115	297.26	L157
309.14	L030	298.95	L073	317.23	L116	298.26	L158
								290.37	L031	299.95	L074	318.23	L117	299.26	L159
291.37	L032	300.95	L075	319.23	L118	300.26	L160
								292.37	L033	301.95	L076	320.23	L119	301.26	L161
293.37	L034	302.95	L077	321.23	L120	302.26	L162

291.14	L012	314.37	L055	299.23	L098	303.26	L140
								294.37	L035	303.95	L078	284.26	L121	303.26	L163
295.37	L036	304.95	L079	285.26	L122	304.26	L164
								296.37	L037	305.95	L080	286.26	L123	305.26	L165
297.37	L038	306.95	L081	287.26	L124	306.26	L166
								298.37	L039	307.95	L082	288.26	L125	307.26	L167
299.37	L040	308.95	L083	289.26	L126	308.26	L168
								300.37	L041	309.95	L084	290.26	L127	309.26	L169
301.37	L042	310.95	L085	291.26	L128	310.26	L170
								302.37	L043	311.95	L086

2, according to the weight of animals, remove body weight the higher person and junior, take out 150;

3, use random sampling method and method of the present invention to divide into groups respectively, be divided into three groups, each treated animal number is respectively: 40,50,60.Calculate every group weight average value, sample the weight average value,

Value.

Use the group result of random sampling method as shown in table 5, average variance between its group Be 1.44746.

According to method of the present invention, the body weight value input computer with 150 rats draws by aforementioned calculation machine program

The sort of body weight value grouping situation of value minimum, according to this body weight value grouping situation 150 rats divide into groupss, the result is as shown in table 6, average variance between it is organized Be 8.91E-03.Illustrate that method of the present invention compares with random sampling method, its variance is much smaller, and is also minimum to the influence of experimental result.

N=3 wherein,

Be every group weight average number,

Weight average number for population sample.

The random packet of table 5,150 rats

First group		Second group		The 3rd group
						Numbering	Body weight (g)	Numbering	Body weight (g)	Numbering	Body weight (g)

First group		Second group		The 3rd group
						1109	308.52	1060	299.36	1044	283.36
1065	304.36	1129	288.46	1141	300.46
						1003	282.12	1128	287.46	1057	296.36
1026	305.12	1117	316.52	1136	295.46
						1126	285.46	1059	298.36	1143	302.46
1030	309.12	1114	313.52	1013	292.12
						1043	282.36	1069	308.36	1019	298.12
1086	285.52	1032	311.12	1061	300.36
						1124	283.46	1070	309.36	1056	295.36
1090	289.52	1116	315.52	1027	306.12
						1022	301.12	1062	301.36	1055	294.36
1111	310.52	1150	309.46	1104	303.52
						1163	282.89	1095	294.52	1029	308.12
1084	283.52	1135	294.46	1113	312.52
						1089	288.52	1125	284.46	1053	292.36
1144	303.46	1147	306.46	1098	297.52
						1021	300.12	1133	292.46	1085	284.52
1047	286.36	1036	315.12	1015	294.12
						1048	287.36	1051	290.36	1093	292.52
1028	307.12	1035	314.12	1006	285.12
						1075	314.36	1132	291.46	1142	301.46
1011	290.12	1149	308.46	1018	297.12

First group		Second group		The 3rd group
						1016	295.12	1106	305.52	1005	284.12
1169	288.89	1099	298.52	1105	304.52
						1148	307.46	1138	297.46	1101	300.52

1145	304.46	1025	304.12	1137	296.46
						1033	312.12	1063	302.36	1009	288.12
1074	313.36	1162	281.89	1151	310.46
						1067	306.36	1010	289.12	1154	313.46
1058	297.36	1164	283.89	1034	313.12
						1155	314.46	1008	287.12	1167	286.89
1077	316.36	1072	311.36	1123	282.46
						1076	315.36	1088	287.52	1007	286.12
1100	299.52	1052	291.36	1091	290.52
						1020	299.12	1110	309.52	1153	312.46
1108	307.52	1066	305.36	1127	286.46
						1023	302.12	1140	299.46	1134	293.46
1064	303.36	1146	305.46	1083	282.52
						1139	298.46	1170	289.89	1014	293.12
1046	285.36	1157	316.46	1096	295.52
								1012	291.12	1092	291.52
		1165	284.89	1107	306.52

1145	304.46	1025	304.12	1137	296.46
								1073	312.36	1071	310.36
		1038	317.12	1102	301.52
								1068	307.36	1087	286.52
		1112	311.52	1094	293.52
								1024	303.12	1115	314.52
		1050	289.36	1131	290.46
								1045	284.36	1152	311.46
		1004	283.12	1156	315.46
										1037	316.12
				1017	296.12
										1168	287.89
				1166	285.89
										1054	293.36
				1097	296.52
										1031	310.12
				1103	302.52
										1130	289.46
				1049	288.36

1145	304.46	1025	304.12	1137	296.46
						This class mean	298.645	This class mean	300.0292	This class mean	297.0661
Sample mean is:	298.5792	Average variance is between group:	1.44746

Table 6,150 rats divide into groups according to method of the present invention

First group

Second group

The 3rd group

Numbering	Body weight (g)	Numbering	Body weight (g)	Numbering	Body weight (g)
						1111	310.52	1046	285.36	1011	290.12
1125	284.46	1140	299.46	1059	298.36
						1005	284.12	1003	282.12	1095	294.52
1053	292.36	1090	289.52	1117	316.52
						1048	287.36	1034	313.12	1154	313.46
1129	288.46	1163	282.89	1153	312.46
						1109	308.52	1063	302.36	1162	281.89
1057	296.36	1087	286.52	1047	286.36
						1052	291.36	1131	290.46	1148	307.46
1028	307.12	1020	299.12	1116	315.52
						1017	296.12	1134	293.46	1141	300.46
1064	303.36	1093	292.52	1136	295.46
						1067	306.36	1032	311.12	1164	283.89
1058	297.36	1155	314.46	1025	304.12
						1049	288.36	1096	295.52	1128	287.46
1094	293.52	1075	314.36	1149	308.46

Numbering	Body weight (g)	Numbering	Body weight (g)	Numbering	Body weight (g)
						1147	306.46	1169	288.89	1157	316.46
1110	309.52	1007	286.12	1123	282.46
						1013	292.12	1033	312.12	1012	291.12
1097	296.52	1015	294.12	1144	303.46
						1145	304.46	1104	303.52	1074	313.36
1115	314.52	1023	302.12	1170	289.89
						1152	311.46	1107	306.52	1008	287.12
1100	299.52	1056	295.36	1006	285.12
						1010	289.12	1077	316.36	1103	302.52
1036	315.12	1035	314.12	1146	305.46
						1031	310.12	1142	301.46	1044	283.36
1030	309.12	1072	311.36	1069	308.36
						1130	289.46	1114	313.52	1139	298.46
1091	290.52	1124	283.46	1070	309.36
						1126	285.46	1071	310.36	1165	284.89
1027	306.12	1018	297.12	1004	283.12
						1167	286.89	1098	297.52	1024	303.12
1054	293.36	1038	317.12	1060	299.36
						1009	288.12	1108	307.52	1102	301.52
1022	301.12	1083	282.52	1073	312.36
						1133	292.46	1061	300.36	1156	315.46
1138	297.46	1112	311.52	1051	290.36

Numbering	Body weight (g)	Numbering	Body weight (g)	Numbering	Body weight (g)
						1037	316.12	1099	298.52	1076	315.36
1066	305.36	1089	288.52	1068	307.36
								1106	305.52	1045	284.36
		1021	300.12	1026	305.12

		1168	287.89	1092	291.52
								1062	301.36	1055	294.36
		1043	282.36	1019	298.12
								1143	302.46	1014	293.12
		1105	304.52	1135	294.46
								1084	283.52	1016	295.12
		1166	285.89	1086	285.52
								1088	287.52	1151	310.46
				1085	284.52
										1150	309.46
				1101	300.52
										1065	304.36
				1132	291.46
										1029	308.12
				1137	296.46
										1127	286.46
				1050	289.36
										1113	312.52

		1168	287.89	1092	291.52
						This class mean	298.6557	This class mean	298.6731	This class mean	298.689
Sample mean is:	298.5792	Average variance is between group:	8.91E-03

Embodiment 3, get 100 from 120 rats, be divided into six groups, each treated animal number is respectively: 15,15,15,25, and 10,20

1,120 rats raising are measured body weight, the result is as shown in table 7:

The body weight of table 7,120 rats

Body weight (g)	Numbering	Body weight (g)	Numbering	Body weight (g)	Numbering	Body weight (g)	Numbering
								286.35	1001	281.11	1031	289.34	1061	286.87	1091
287.35	1002	282.11	1032	290.34	1062	287.87	1092
								288.35	1003	283.11	1033	291.34	1063	288.87	1093
289.35	1004	284.11	1034	292.34	1064	289.87	1094
								290.35	1005	285.11	1035	293.34	1065	290.87	1095
291.35	1006	286.11	1036	294.34	1066	291.87	1096
								292.35	1007	287.11	1037	295.34	1067	292.87	1097
293.35	1008	288.11	1038	296.34	1068	293.87	1098
								294.35	1009	289.11	1039	297.34	1069	294.87	1099
295.35	1010	290.11	1040	298.34	1070	295.87	1100
								296.35	1011	291.11	1041	299.34	1071	296.87	1101
297.35	1012	292.11	1042	300.34	1072	297.87	1102

298.35	1013	293.11	1043	301.34	1073	298.87	1103
								299.35	1014	294.11	1044	302.34	1074	299.87	1104

298.35	1013	293.11	1043	301.34	1073	298.87	1103
								300.35	1015	295.11	1045	303.34	1075	300.87	1105
301.35	1016	296.11	1046	304.34	1076	301.87	1106
								302.35	1017	297.11	1047	305.34	1077	302.87	1107
303.35	1018	298.11	1048	306.34	1078	303.87	1108
								304.35	1019	299.11	1049	307.34	1079	304.87	1109
305.35	1020	300.11	1050	308.34	1080	305.87	1110
								306.35	1021	301.11	1051	309.34	1081	306.87	1111
307.35	1022	302.11	1052	310.34	1082	307.87	1112
								308.35	1023	303.11	1053	311.34	1083	308.87	1113
309.35	1024	304.11	1054	312.34	1084	309.87	1114
								310.35	1025	305.11	1055	313.34	1085	310.87	1115
311.35	1026	306.11	1056	314.34	1086	311.87	1116
								312.35	1027	307.11	1057	315.34	1087	312.87	1117
313.35	1028	308.11	1058	316.34	1088	313.87	1118
								314.35	1029	309.11	1059	317.34	1089	314.87	1119
315.35	1030	310.11	1060	318.34	1090	315.87	1120

2, according to the weight of animals, remove body weight the higher person and junior, take out 100;

3, use random sampling method and method of the present invention to divide into groups respectively, be divided into six groups, each treated animal number is respectively: 15,15,15,25, and 10,20.Calculate every group weight average value, sample the weight average value,

Value.

Use the group result of random sampling method as shown in table 8, average variance between its group

Be 12.2231.

According to method of the present invention, with the body weight value of 100 rats, the input computer draws by aforementioned calculation machine program The sort of body weight value grouping situation of value minimum, according to this body weight value grouping situation 100 rats divide into groupss, the result is as shown in table 9, average variance between it is organized

Be 0.038121.Illustrate that method of the present invention compares with random sampling method, its variance is much smaller, and is also minimum to the influence of experimental result.

N=6 wherein, Be every group weight average number,

Weight average number for population sample.

The random packet of table 8,100 rats

First group		Second group		The 3rd group		The 4th group		The 5th group		The 6th group
												Numbering	Body weight (g)	Numbering	Body weight (g)	Numbering	Body weight (g)	Numbering	Body weight (g)	Numbering	Body weight (g)	Numbering	Body weight (g)
1089	288.52	1032	311.12	1096	295.52	1013	292.12	1061	300.36	1051	290.36
												1086	285.52	1101	300.52	1111	310.52	1085	284.52	1094	293.52	1106	305.52
1072	311.36	1110	309.52	1102	301.52	1088	287.52	1028	307.12	1004	283.12
												1052	291.36	1023	302.12	1115	314.52	1035	314.12	1007	286.12	1044	283.36
1046	285.36	1104	303.52	1114	313.52	1108	307.52	1022	301.12	1092	291.52
												1076	315.36	1011	290.12	1107	306.52	1029	308.12	1090	289.52	1070	309.36
1009	288.12	1033	312.12	1099	298.52	1071	310.36	1018	297.12	1054	293.36
												1055	294.36	1109	308.52	1063	302.36	1098	297.52	1010	289.12	1027	306.12
1005	284.12	1066	305.36	1021	300.12	1020	299.12	1049	288.36	1012	291.12
												1084	283.52	1037	316.12	1091	290.52	1014	293.12	1068	307.36	1053	292.36
1067	306.36	1048	287.36	1112	311.52	1057	296.36			1075	314.36
												1059	298.36	1019	298.12	1030	309.12	1062	301.36			1069	308.36
1074	313.36	1034	313.12	1036	315.12	1116	315.52			1103	302.52
												1097	296.52	1016	295.12	1073	312.36	1087	286.52			1025	304.12
1064	303.36	1058	297.36	1017	296.12	1100	299.52			1024	303.12
																		1006	285.12			1026	305.12
						1031	310.12			1056	295.36
																		1095	294.52			1065	304.36

First group		Second group		The 3rd group		The 4th group		The 5th group		The 6th group
																		1093	292.52			1008	287.12
						1060	299.36			1047	286.36
																		1045	284.36
						1113	312.52
																		1050	289.36
						1105	304.52
																		1015	294.12
This class mean	296.2199	This class mean	303.1906	This class mean	305.0413	This class mean	298.2429	This class mean	295.8213	This class mean	297.6993
												Sample mean is:	299.3692	Average variance is between group:	12.2231

Table 9,100 rats divide into groups according to method of the present invention

First group		Second group		The 3rd group		The 4th group		The 5th group		The 6th group
												Numbering	Body weight (g)	Numbering	Body weight (g)	Numbering	Body weight (g)	Numbering	Body weight (g)	Numbering	Body weight (g)	Numbering	Body weight (g)
1014	293.12	1037	316.12	1046	285.36	1013	292.12	1100	299.52	1101	300.52
												1072	311.36	1055	294.36	1015	294.12	1010	289.12	1096	295.52	1018	297.12
1017	296.12	1088	287.52	1005	284.12	1074	313.36	1050	289.36	1087	286.52
												1111	310.52	1066	305.36	1060	299.36	1048	287.36	1029	308.12	1035	314.12
1054	293.36	1068	307.36	1024	303.12	1094	293.52	1107	306.52	1086	285.52

1116	315.52	1019	298.12	1049	288.36	1103	302.52	1097	296.52	1031	310.12
												1012	291.12	1025	304.12	1114	313.52	1061	300.36	1093	292.52	1034	313.12
1009	288.12	1105	304.52	1091	290.52	1106	305.52	1053	292.36	1089	288.52
												1064	303.36	1102	301.52	1036	315.12	1023	302.12	1112	311.52	1011	290.12

1116	315.52	1019	298.12	1049	288.36	1103	302.52	1097	296.52	1031	310.12
												1008	287.12	1099	298.52	1113	312.52	1085	284.52	1065	304.36	1020	299.12
1110	309.52	1063	302.36	1044	283.36	1109	308.52			1071	310.36
												1007	286.12	1062	301.36	1028	307.12	1004	283.12			1021	300.12
1006	285.12	1045	284.36	1092	291.52	1057	296.36			1052	291.36
												1070	309.36	1058	297.36	1030	309.12	1115	314.52			1095	294.52
1075	314.36	1090	289.52	1073	312.36	1051	290.36			1067	306.36
																		1033	312.12			1016	295.12
						1059	298.36			1022	301.12
																		1069	308.36			1032	311.12
						1108	307.52			1047	286.36
																		1084	283.52			1026	305.12
						1104	303.52
																		1076	315.36
						1056	295.36
																		1098	297.52
						1027	306.12
												This class mean	299.6133	This class mean	299.4986	This class mean	299.3067	This class mean	299.6464	This class mean	299.632	This class mean	299.318
Sample mean is:	299.3692	Average variance is between group:	0.038121

Claims (5)

1. method that laboratory animal is divided into groups may further comprise the steps:

1) measures each individual value of statistical indicant in the animal specimen of waiting to divide into groups;

2) number of individuals of the group number that requires according to zoopery and every group is to 1) in all individual values of statistical indicant divide into groups, find out The sort of value of statistical indicant grouping situation of value minimum; Wherein n is the group number of requirement of experiment,

Be every group value of statistical indicant average,

Value of statistical indicant average for sample;

The sort of value of statistical indicant grouping situation of value minimum laboratory animal is divided into groups.

2. method according to claim 1 is characterized in that: described laboratory animal is used for the drug toxicity research experiment.

3. according to the described method of claim l, it is characterized in that: described laboratory animal is used for the drug metabolism research experiment.

4. method according to claim 1 is characterized in that: described laboratory animal is used for the animal grouping that medicine effect is learned research experiment.

5. according to claim 1,2,3 or 4 described methods, it is characterized in that: described sign is selected from any one in body weight, blood pressure and the body surface area.

Images