CN1928550A - Thin-layered chromatography detection method for Sudan pigment in red pepper products - Google Patents
Thin-layered chromatography detection method for Sudan pigment in red pepper products Download PDFInfo
- Publication number
- CN1928550A CN1928550A CNA2005100179690A CN200510017969A CN1928550A CN 1928550 A CN1928550 A CN 1928550A CN A2005100179690 A CNA2005100179690 A CN A2005100179690A CN 200510017969 A CN200510017969 A CN 200510017969A CN 1928550 A CN1928550 A CN 1928550A
- Authority
- CN
- China
- Prior art keywords
- sudan
- pigment
- sample
- thin
- sherwood oil
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
The disclosed thin-layer chromatography (TLC) method for Sudan pigment in capsicum product comprises: extracting pigment by petroleum ether for power or by active clay for oil, desorbing the pigment by anhydrous alcohol, fixing volume by petroleum ether, and analyzing by TLC. This invention is convenient and low cost, overcomes defects in prior art, improves detection effect, and has bright application future.
Description
Technical field
The present invention relates to a kind of method that whether contains the Sudan's pigment in the chilli products that detects, the thin-layered chromatography detection method of the Sudan's pigment in particularly a kind of capsicum powder and the chilli oil.
Background technology
The Sudan's pigment is the lipophilicity azo-compound, mainly comprises four types of I, II, III and IV.The Sudan's pigment that enters in the body mainly carries out metabolism by intestines and stomach micro-reduction enzyme, liver and extrahepatic tissue microsome and cytoplasmic reductase, is metabolized to corresponding amine substance in vivo.The mutagenicity of finding the Sudan's pigment in multinomial external mutagenicity test and animal carcinogenic test is relevant with the amine substance of metabolism generation with carcinogenicity.The Sudan's pigment is a kind of a kind of industrial dye of synthetic, and countries such as (EU) of European Union had forbidden that it added in food as pigment in 2003, and this China is also expressly forbidden.
In March, 2005, sudan detects in China's chilli products first as the violation food additives, but relevant effective measuring means but seldom, can not popularize.Method is as follows: high performance liquid chromatography, the merging of diode array detector and ultraviolet spectrophotometer is used, spectrophotometric method.The present domestic additive method that also do not have, and the major defect of these methods is that operation is cumbersome, instrument condition requires height, it is backward to be unfavorable for that department's instrument detects in vast at home basic unit, the general environment on the low side of operator quality is promoted down, is difficult to satisfy the needs to the field management of chilli products production and selling.Along with the government and the people more and more pay attention to food security, the low-cost discrimination method that whether adds the Sudan's pigment in the research chilli products seems necessary and urgent.
Summary of the invention
The purpose of this invention is to provide a kind of simple to operate and sample and differentiate the method that detects the Sudan's pigment in the chilli products accurately.
For achieving the above object, the present invention adopts following technical scheme: the thin-layered chromatography detection method of the Sudan's pigment in a kind of capsicum powder, testing process is operated as follows: (1) takes by weighing an amount of capsicum powder in beaker, add and leave standstill after extract mixes, precipitate fully until insolubles, pour out supernatant; (2), add an amount of extract and dissolve standby with the above-mentioned liquid evaporate to dryness that extracts; (3) make the Sudan's pigment titer, get the Sudan's pigment and place volumetric flask extract constant volume, be made into the Sudan's pigment titer; (4) draw the Sudan's pigment titer point sample on thin layer plate that the standby sample that makes in the step 2 and step 3 make, in developping agent, launch, according to sample on the chromatographic sheet and each the Sudan's pigment standard sample spot comparative analysis.
The described extract of epimere is a sherwood oil, and described thin layer plate is silica gel thin-layer plate (Si60F
254), described developping agent is the mixed liquor of sherwood oil, absolute ether and formic acid.
The volume ratio of each component is a sherwood oil in the described developping agent mixed liquor: absolute ether: formic acid=(65～90): (10～35): 1.
Detect and take by weighing the commercial capsicum powder of 2.8 grams in the capsicum described step of powder operating process (1) in beaker, adding the 10ml sherwood oil left standstill 30 minutes after mixing 3 minutes on the eddy mixer, precipitate fully until insolubles, pour out supernatant, then repeat aforesaid operations 2 times; Step (2) is the above-mentioned liquid mixing that extracts, evaporate to dryness, and it is standby to add 100～250ml petroleum ether dissolution; Step (3) takes by weighing the Sudan I pigment 0.05 gram and places 100ml volumetric flask sherwood oil constant volume, is made into the Sudan I pigment titer of 0.5g/L; Each 3～5 μ l point sample on described silica gel thin-layer plate of the Sudan I pigment titer that standby sample that makes in step (4) the absorption step 2 and step 3 make, in described developping agent, launch, according to each sample on the chromatographic sheet and the comparative analysis of the Sudan I pigment standard sample spot.
The thin-layered chromatography detection method of the Sudan's pigment in a kind of chilli oil, testing process is operated as follows: (1) is got the Sudan's pigment and use the extract constant volume in volumetric flask, and it is standby to be made into standard the Sudan pigment sample; (2) get an amount of chilli oil in beaker, add extract, toward beaker in, add adsorbent again, stir and pass through filter paper filtering with dilute solution, and with extract wash to filtrate colourless till; (3) adsorbent is collected, filtered with the strippant desorb, till stripping liquid is colourless; (4) collect stripping liquid, evaporate to dryness, standby with the extract constant volume in volumetric flask; (5) draw the Sudan's pigment titer point sample on thin layer plate that the standby sample that makes in the step 4 and step 1 make, and in developping agent, launch, according to each sample on the chromatographic sheet and the Sudan's pigment standard sample spot comparative analysis.
The described extract of epimere is a sherwood oil, and described adsorbent is an atlapulgite, and described strippant is an absolute ethyl alcohol, and described thin layer plate is silica gel thin-layer plate (Si60F
254), described developping agent is the mixed liquor of sherwood oil, absolute ether and formic acid.
The volume ratio of each component is a sherwood oil in the described developping agent mixed liquor: absolute ether: formic acid=(65～90): (10～35): 1.
Detect the described step of chilli oil operating process (1) and respectively get the Sudan I-IV 0.005g use the sherwood oil constant volume in the 100ml volumetric flask, it is standby to be made into standard the Sudan I-IV recombined sample; Step (2) is got the commercial chilli oil of 10ml in the 100ml beaker, the sherwood oil that adds 10ml is with dilute solution, the atlapulgite that adds 1～1.5g again in the beaker, filter paper filtering is passed through in stirring, and with sherwood oil wash to filtrate colourless till, collect filtrate,, repeat aforesaid operations to the atlapulgite that wherein adds 1g; Step (3) is collected the atlapulgite of twice use, filters with the absolute ethyl alcohol desorb, till stripping liquid is colourless; Step (4) is collected stripping liquid, and evaporate to dryness is standby with the sherwood oil constant volume in the 10ml volumetric flask; Each 3～5 μ l point sample on silica gel thin-layer plate of the Sudan's pigment titer that standby sample that makes in step (5) the absorption step 4 and step 1 make, in described developping agent, launch, according to each sample on the chromatographic sheet and the Sudan's pigment standard sample spot comparative analysis.
The present invention is used for detecting the advantage whether chilli products contain the thin-layered chromatography detection method of the Sudan's pigment: simple to operate, cheap, and less demanding to instrument and equipment; Sample is differentiated accurately, good reproducibility; Simple to operate, can be used for the production scene of chilli products easily and sell on-the-spot supervision and management, when having overcome traditional liquid phase chromatography and having detected chilli products to instrument requirement condition height, it is backward to be unfavorable for that department's instrument detects in vast at home basic unit, deficiencies such as the following popularization of the general environment on the low side of operator quality have good use prospect.
Description of drawings
Figure 1 shows that an embodiment chromatogram that detects chilli powder;
Figure 2 shows that second the embodiment chromatogram that detects chilli powder;
Figure 3 shows that the 3rd the embodiment chromatogram that detects chilli powder;
Figure 4 shows that an embodiment chromatogram that detects chilli oil;
Figure 5 shows that second the embodiment chromatogram that detects chilli oil;
Figure 6 shows that the 3rd the embodiment chromatogram that detects chilli oil.
Embodiment
Step 1: take by weighing the commercial capsicum powder of 2.8 grams in beaker, add the 10ml sherwood oil and left standstill 30 minutes after mixing 3 minutes on the eddy mixer, precipitate fully until insolubles, pour out supernatant, it is thin out to the capsicum color then to repeat aforesaid operations 2 times.
Step 2: with the above-mentioned liquid mixing that extracts, evaporate to dryness, adding 100ml petroleum ether dissolution is standby.
Step 3: take by weighing the Sudan I pigment 0.05 gram and place 100ml volumetric flask sherwood oil constant volume, be made into the Sudan I pigment titer of 0.5g/L.
Step 4: each 3 μ l point sample on silica gel thin-layer plate of the Sudan I pigment titer that standby sample that makes in the absorption step 2 and step 3 make, the composition of described silica gel thin-layer plate is Si60F
254, produce by MERCK GERMANY.Launch in developping agent, described developping agent is the mixed liquor of sherwood oil, absolute ether and formic acid, and the volume ratio of each component is a sherwood oil in the mixed liquor: absolute ether: formic acid=65: 35: 1.
As shown in Figure 1, according to sample on the chromatographic sheet and the comparative analysis of the Sudan I pigment standard sample spot, color spot 1 is a Sudan I pigment standard sample spot, color spot 2 is the pigment spots that extract from commercial chilli powder, the height of contrast color spot 2 and color spot 1, find not to be positioned in the color spot 2 spot of equal height, illustrate not contain the Sudan I pigment in this chilli powder with color spot 1.
Step 1: take by weighing 2.97 grams among the embodiment 1 the commercial capsicum powder of same bottle in beaker, adding 0.11 gram the Sudan I fully mixes, adding the 10ml sherwood oil again left standstill 30 minutes after mixing 3 minutes on the eddy mixer, precipitate fully until insolubles, pour out supernatant, it is thin out to the capsicum color then to repeat aforesaid operations 2 times.
Step 2: with the above-mentioned liquid mixing that extracts, evaporate to dryness, adding 250ml petroleum ether dissolution is standby.
Step 3: take by weighing the Sudan I pigment 0.05 gram and place 100ml volumetric flask sherwood oil constant volume, be made into the Sudan I pigment titer of 0.5g/L.
Step 4: each 5 μ l point sample on silica gel thin-layer plate of the Sudan I pigment titer that standby sample that makes in the absorption step 2 and step 3 make, the composition of described silica gel thin-layer plate is Si60F
254, produce by MERCK GERMANY.Launch in developping agent, described developping agent is the mixed liquor of sherwood oil, absolute ether and formic acid, and the volume ratio of each component is a sherwood oil in the mixed liquor: absolute ether: formic acid=90: 10: 1.
As shown in Figure 2, according to sample on the chromatographic sheet and the comparative analysis of the Sudan I pigment standard sample spot, color spot 1 is a Sudan I pigment standard sample spot, color spot 2 is the pigment spots that extract from the commercial chilli powder that added the Sudan I pigment, the height of contrast color spot 2 and color spot 1, finding has the spot that is positioned at equal height with color spot 1 in the color spot 2, illustrates and contains in the color spot 2 and the identical material of the Sudan's I Rf value, thereby prove and contain the Sudan I pigment in this chilli powder.
Step 1: 1., take by weighing 2.8 grams among the embodiment 1 the commercial capsicum powder of same bottle in beaker, adding the 10ml sherwood oil left standstill 30 minutes after mixing 3 minutes on the eddy mixer, precipitate fully until insolubles, pour out supernatant, it is thin out to the capsicum color then to repeat aforesaid operations 2 times.2., take by weighing 2.97 grams among the embodiment 1 the commercial capsicum powder of same bottle in beaker, adding 0.11 gram the Sudan I fully mixes, adding the 10ml sherwood oil again left standstill 30 minutes after mixing 3 minutes on the eddy mixer, precipitate fully until insolubles, pour out supernatant, it is thin out to the capsicum color then to repeat aforesaid operations 2 times.
Step 2: with the liquid mixing that step 1.1 extracted, evaporate to dryness, it is standby that adding 200ml petroleum ether dissolution is made sample 1.With the liquid mixing that step 1.2 extracted, evaporate to dryness, it is standby that adding 200ml petroleum ether dissolution is made sample 2.
Step 3: take by weighing the Sudan I pigment 0.05 gram and place 100ml volumetric flask sherwood oil constant volume, be made into the Sudan I pigment titer of 0.5g/L.
Step 4: each 4 μ l point sample on silica gel thin-layer plate of the Sudan I pigment titer that standby sample that makes in the absorption step 2 and step 3 make, the composition of described silica gel thin-layer plate is Si60F
254, produce by MERCK GERMANY.Launch in developping agent, described developping agent is the mixed liquor of sherwood oil, absolute ether and formic acid, and the volume ratio of each component is a sherwood oil in the mixed liquor: absolute ether: formic acid=80: 20: 1.
As shown in Figure 3, according to sample on the chromatographic sheet and the comparative analysis of the Sudan I pigment standard sample spot, color spot 1 is a Sudan I pigment standard sample spot, and color spot 2 is contained pigment spots in the sample 1, and color spot 3 is contained pigment spots in the sample 2.Find through contrast, be not positioned at the spot of equal height in the color spot 2, illustrate not contain the Sudan I pigment in this chilli powder with color spot 1; The height of contrast color spot 3 and color spot 1, finding has the spot that is positioned at equal height with color spot 1 in the color spot 3, illustrates and contains in the color spot 3 and the identical material of the Sudan's I Rf value, thereby prove and added the Sudan I pigment in this chilli powder.
Step 1: respectively get the Sudan I-IV 0.005g respectively at using the sherwood oil constant volume in the 100ml volumetric flask, it is standby to be made into standard the Sudan I-IV sample.Get the Sudan I-IV 0.005g more respectively and be mixed in and use the sherwood oil constant volume in the 100ml volumetric flask, it is standby to be made into standard the Sudan I-IV recombined sample.
Step 2: get the commercial chilli oil of 10ml in the 100ml beaker, the sherwood oil that adds 10ml is with dilute solution, the atlapulgite that adds 1g again in the beaker, filter paper filtering is passed through in stirring, and with sherwood oil wash to filtrate colourless till, collect filtrate,, repeat aforesaid operations to the atlapulgite that wherein adds 1g.
Step 3: the atlapulgite of twice use is collected, filtered with the absolute ethyl alcohol desorb, till stripping liquid is colourless.
Step 4: collect stripping liquid, evaporate to dryness, standby with the sherwood oil constant volume in the 10ml volumetric flask.
Step 5: the Sudan's pigment titer that standby sample that makes in the absorption step 4 and step 1 make and each 3 μ l point sample on silica gel thin-layer plate of the Sudan I-IV hybrid standard liquid, the composition of described silica gel thin-layer plate is Si60F
254, produce by MERCK GERMANY.Launch in developping agent, described developping agent is the mixed liquor of sherwood oil, absolute ether and formic acid, and the volume ratio of each component is a sherwood oil in the mixed liquor: absolute ether: formic acid=70: 30: 1.
As shown in Figure 4, according to sample on the chromatographic sheet and the Sudan I, II, III, IV pigment standard sample spot and the comparative analysis of the Sudan I-IV hybrid standard sample spot, color spot 1 is a Sudan I pigment standard sample spot, color spot 2 is a Sudan II pigment standard sample spot, color spot 3 is a soudan III pigment standard sample spot, color spot 4 is a Sudan IV pigment standard sample spot, color spot 5 is a Sudan I-IV pigment hybrid standard sample spot, color spot 6 is the pigment spots that extract from commercial chilli oil, contrast color spot 6 and color spot 1,2,3,4,5 height, finding does not have and color spot 1 in the color spot 6,2,3,4,5 are positioned at the spot of equal height, illustrate not contain the Sudan I in this chilli oil, II, III, the IV pigment.
Step 1: respectively get the Sudan I-IV 0.005g respectively at using the sherwood oil constant volume in the 100ml volumetric flask, it is standby to be made into standard the Sudan I-IV sample.Get the Sudan I-IV 0.005g more respectively and be mixed in and use the sherwood oil constant volume in the 100ml volumetric flask, it is standby to be made into standard the Sudan I-IV recombined sample.
Step 2: get among the embodiment 4 the commercial chilli oil 10ml of same bottle in the 100ml beaker, other gets the Sudan I pigment 0.005g and adds wherein mixing, the sherwood oil that adds 10ml is with dilute solution, the atlapulgite that adds 1.3g again in the beaker, stir by filter paper filtering, and with sherwood oil wash to filtrate colourless till, collect filtrate, to the atlapulgite that wherein adds 1g, repeat aforesaid operations.This moment, filtrate was orange-yellow, and atlapulgite is aubergine.
Step 3: the atlapulgite of twice use is collected, filtered with the absolute ethyl alcohol desorb, till stripping liquid is colourless.Atlapulgite will recover original color this moment.
Step 4: collect stripping liquid, evaporate to dryness, standby with the sherwood oil constant volume in the 10ml volumetric flask.
Step 5: the Sudan's pigment titer that standby sample that makes in the absorption step 4 and step 1 make and each 4 μ l point sample on silica gel thin-layer plate of the Sudan I-IV hybrid standard liquid, the composition of described silica gel thin-layer plate is Si60F
254, produce by MERCK GERMANY.Launch in developping agent, described developping agent is the mixed liquor of sherwood oil, absolute ether and formic acid, and the volume ratio of each component is a sherwood oil in the mixed liquor: absolute ether: formic acid=85: 15: 1.
As shown in Figure 5, according to sample on the chromatographic sheet and the Sudan I, II, III, IV pigment standard sample spot and the comparative analysis of the Sudan I-IV hybrid standard sample spot, color spot 1 is a Sudan I pigment standard sample spot, color spot 2 is a Sudan II pigment standard sample spot, color spot 3 is a soudan III pigment standard sample spot, color spot 4 is a Sudan IV pigment standard sample spot, color spot 5 is a Sudan I-IV pigment hybrid standard sample spot, color spot 6 is the pigment spots that extract from the commercial chilli oil that has added the Sudan I pigment, contrast color spot 6 and color spot 1,2,3,4,5 height, find that top second spot is positioned at equal height in color spot 6 and color spot 1 and the color spot 5, illustrate and contain in the color spot 6 and the identical material of the Sudan's I Rf value, thereby prove and contain the Sudan I pigment in this chilli oil.
Step 1: respectively get the Sudan I-IV 0.005g respectively at using the sherwood oil constant volume in the 100ml volumetric flask, it is standby to be made into standard the Sudan I-IV sample.Get the Sudan I-IV 0.005g more respectively and be mixed in and use the sherwood oil constant volume in the 100ml volumetric flask, it is standby to be made into standard the Sudan I-IV recombined sample.
Step 2: get among the embodiment 4 the commercial chilli oil 10ml of same bottle in the 100ml beaker, respectively get the Sudan I-IV 0.005g in addition and add wherein mixing, the sherwood oil that adds 10ml is with dilute solution, the atlapulgite that adds 1.5g again in the beaker, stir by filter paper filtering, and with sherwood oil wash to filtrate colourless till, collect filtrate, to the atlapulgite that wherein adds 1g, repeat aforesaid operations.This moment, filtrate was orange-yellow, and atlapulgite is aubergine.
Step 3: the atlapulgite of twice use is collected, filtered with the absolute ethyl alcohol desorb, till stripping liquid is colourless.Atlapulgite will recover original color this moment.
Step 4: collect stripping liquid, evaporate to dryness, standby with the sherwood oil constant volume in the 10ml volumetric flask.
Step 5: the Sudan's pigment titer that standby sample that makes in the absorption step 4 and step 1 make and each 5 μ l point sample on silica gel thin-layer plate of the Sudan I-IV hybrid standard liquid, the composition of described silica gel thin-layer plate is Si60F
254, produce by MERCK GERMANY.Launch in developping agent, described developping agent is the mixed liquor of sherwood oil, absolute ether and formic acid, and the volume ratio of each component is a sherwood oil in the mixed liquor: absolute ether: formic acid=78: 22: 1.
As shown in Figure 5, according to sample on the chromatographic sheet and the Sudan I, II, III, IV pigment standard sample spot and the comparative analysis of the Sudan I-IV hybrid standard sample spot, color spot 1 is a Sudan I pigment standard sample spot, color spot 2 is a Sudan II pigment standard sample spot, color spot 3 is a soudan III pigment standard sample spot, color spot 4 is a Sudan IV pigment standard sample spot, color spot 5 is a Sudan I-IV pigment hybrid standard sample spot, color spot 6 is from having added the Sudan I, II, III, the pigment spot that extracts in the commercial chilli oil of IV pigment, contrast color spot 6 and color spot 1,2,3,4,5 height, find in the color spot 6 and color spot 1,2,3,4,5 spots that are positioned at equal height have, illustrate and contain with the Sudan I in the color spot 6, II, III, the material that the IV Rf value is identical, thus prove and contain the Sudan I in this chilli oil, II, III, the IV pigment.
Claims (8)
1, the thin-layered chromatography detection method of the Sudan's pigment in a kind of chilli products, described chilli products is the capsicum powder, it is characterized in that testing process operates as follows: (1) takes by weighing an amount of capsicum powder in beaker, add and leave standstill after extract mixes, precipitate fully until insolubles, pour out supernatant; (2), add an amount of extract and dissolve standby with the above-mentioned liquid evaporate to dryness that extracts; (3) make the Sudan's pigment titer, get the Sudan's pigment and place volumetric flask extract constant volume, be made into the Sudan's pigment titer; (4) draw the Sudan's pigment titer point sample on thin layer plate that the standby sample that makes in the step 2 and step 3 make, in developping agent, launch, according to sample on the chromatographic sheet and each the Sudan's pigment standard sample spot comparative analysis.
2, the thin-layered chromatography detection method of the Sudan's pigment in the chilli products as claimed in claim 1, it is characterized in that: described extract is a sherwood oil, described thin layer plate is silica gel thin-layer plate (Si 60 F
254), described developping agent is the mixed liquor of sherwood oil, absolute ether and formic acid.
3, the thin-layered chromatography detection method of the Sudan's pigment in the chilli products as claimed in claim 2, it is characterized in that: the volume ratio of each component is a sherwood oil in the described developping agent mixed liquor: absolute ether: formic acid=(65～90): (10～35): 1.
4, the thin-layered chromatography detection method of the Sudan's pigment in the chilli products as claimed in claim 3, it is characterized in that: take by weighing the commercial capsicum powder of 2.8 grams in the described step (1) in beaker, adding the 10ml sherwood oil left standstill 30 minutes after mixing 3 minutes on the eddy mixer, precipitate fully until insolubles, pour out supernatant, then repeat aforesaid operations 2 times; Step (2) is the above-mentioned liquid mixing that extracts, evaporate to dryness, and it is standby to add 100～250ml petroleum ether dissolution; Step (3) takes by weighing the Sudan I pigment 0.05 gram and places 100ml volumetric flask sherwood oil constant volume, is made into the Sudan I pigment titer of 0.5g/L; Each 3～5 μ l point sample on described silica gel thin-layer plate of the Sudan I pigment titer that standby sample that makes in step (4) the absorption step 2 and step 3 make, in described developping agent, launch, according to each sample on the chromatographic sheet and the comparative analysis of the Sudan I pigment standard sample spot.
5, the thin-layered chromatography detection method of the Sudan's pigment in a kind of chilli products, described chilli products is a chilli oil, it is characterized in that testing process operates as follows: (1) is got the Sudan's pigment and use the extract constant volume in volumetric flask, and it is standby to be made into standard the Sudan pigment sample; (2) get an amount of chilli oil in beaker, add extract, toward beaker in, add adsorbent again, stir and pass through filter paper filtering with dilute solution, and with extract wash to filtrate colourless till; (3) adsorbent is collected, filtered with the strippant desorb, till stripping liquid is colourless; (4) collect stripping liquid, evaporate to dryness, standby with the extract constant volume in volumetric flask; (5) draw the Sudan's pigment titer point sample on thin layer plate that the standby sample that makes in the step 4 and step 1 make, and in developping agent, launch, according to each sample on the chromatographic sheet and the Sudan's pigment standard sample spot comparative analysis.
6, the thin-layered chromatography detection method of the Sudan's pigment in the chilli products as claimed in claim 5, it is characterized in that: described extract is a sherwood oil, and described adsorbent is an atlapulgite, and described strippant is an absolute ethyl alcohol, and described thin layer plate is silica gel thin-layer plate (Si 60 F
254), described developping agent is the mixed liquor of sherwood oil, absolute ether and formic acid.
7, the thin-layered chromatography detection method of the Sudan's pigment in the chilli products as claimed in claim 6, it is characterized in that: the volume ratio of each component is a sherwood oil in the described developping agent mixed liquor: absolute ether: formic acid=(65～90): (10～35): 1.
8, the thin-layered chromatography detection method of the Sudan's pigment in the chilli products as claimed in claim 7, it is characterized in that: described step (1) is respectively got the Sudan I-IV 0.005g and use the sherwood oil constant volume in the 100ml volumetric flask, and it is standby to be made into standard the Sudan I-IV recombined sample; Step (2) is got the commercial chilli oil of 10ml in the 100ml beaker, the sherwood oil that adds 10ml is with dilute solution, the atlapulgite that adds 1～1.5g again in the beaker, filter paper filtering is passed through in stirring, and with sherwood oil wash to filtrate colourless till, collect filtrate,, repeat aforesaid operations to the atlapulgite that wherein adds 1g; Step (3) is collected the atlapulgite of twice use, filters with the absolute ethyl alcohol desorb, till stripping liquid is colourless; Step (4) is collected stripping liquid, and evaporate to dryness is standby with the sherwood oil constant volume in the 10ml volumetric flask; Each 3～5 μ l point sample on silica gel thin-layer plate of the Sudan's pigment titer that standby sample that makes in step (5) the absorption step 4 and step 1 make, in described developping agent, launch, according to each sample on the chromatographic sheet and the Sudan's pigment standard sample spot comparative analysis.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100179690A CN100370254C (en) | 2005-09-05 | 2005-09-05 | Thin-layered chromatography detection method for Sudan pigment in red pepper products |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100179690A CN100370254C (en) | 2005-09-05 | 2005-09-05 | Thin-layered chromatography detection method for Sudan pigment in red pepper products |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1928550A true CN1928550A (en) | 2007-03-14 |
| CN100370254C CN100370254C (en) | 2008-02-20 |
Family
ID=37858611
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2005100179690A Expired - Fee Related CN100370254C (en) | 2005-09-05 | 2005-09-05 | Thin-layered chromatography detection method for Sudan pigment in red pepper products |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN100370254C (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100494976C (en) * | 2007-04-17 | 2009-06-03 | 广东省疾病预防控制中心 | Method for detecting fat-soluble azo dyes in food or fodder and its kit |
| CN101067620B (en) * | 2007-06-08 | 2010-12-08 | 重庆市计量质量检测研究院 | Fast screening reagent kit and method for sudan dye in hot pepper products |
| CN107064401A (en) * | 2016-09-30 | 2017-08-18 | 中国人民解放军第二军医大学 | A kind of quick method for detecting ten kinds of pigments of illegal addition in beverage simultaneously |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4088753A (en) * | 1975-04-16 | 1978-05-09 | Parmer Laurence Pierpont | Method of obtaining a splenic composition which inhibits platelet function and said composition |
| CN1594355B (en) * | 2004-06-18 | 2010-12-08 | 高华 | Cyclovirobuxinum D crystal, its mono-methanol crystal, and preparation and use thereof |
-
2005
- 2005-09-05 CN CNB2005100179690A patent/CN100370254C/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100494976C (en) * | 2007-04-17 | 2009-06-03 | 广东省疾病预防控制中心 | Method for detecting fat-soluble azo dyes in food or fodder and its kit |
| CN101067620B (en) * | 2007-06-08 | 2010-12-08 | 重庆市计量质量检测研究院 | Fast screening reagent kit and method for sudan dye in hot pepper products |
| CN107064401A (en) * | 2016-09-30 | 2017-08-18 | 中国人民解放军第二军医大学 | A kind of quick method for detecting ten kinds of pigments of illegal addition in beverage simultaneously |
Also Published As
| Publication number | Publication date |
|---|---|
| CN100370254C (en) | 2008-02-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109406474A (en) | A kind of aggregation-induced emission-molecular engram fluorescent optical sensor preparation method and application detecting rhodamine B | |
| CN1752750A (en) | Reagent for detecting mercury ion in water and its preparation method | |
| CN1959406A (en) | Method for detecting residue of Metalaxyl, badistan, thiophanate-methyl in vegetable | |
| CN107449850B (en) | Method for determining alkylphenol in environmental water sample by combining fast synergistic cloud point extraction and high performance liquid chromatography | |
| CN113567401B (en) | Rapid detection method and application of landfill leachate polluted underground water condition | |
| CN104749290A (en) | High performance liquid chromatography determination method for identifying starch syrup adulteration in honey | |
| CN103756004A (en) | Preparation method of quantum-dot phosphorescent imprinted polymer | |
| CN1928550A (en) | Thin-layered chromatography detection method for Sudan pigment in red pepper products | |
| CN106706829A (en) | Method for measuring diarrhetic shellfish poisons in shellfishes by use of immunoaffinity purification-liquid chromatography-tandem mass spectrometry | |
| CN115267017B (en) | Method for detecting organic tin by combining dispersed solid phase extraction material and GC-MS | |
| CN107589098B (en) | Method for fluorescence detection of trace uranyl ions | |
| CN109364880A (en) | It is a kind of with circulation parsing function supermolecule organic framework materials and removal water body in organic dyestuff application | |
| CN104568925A (en) | Streptomycin sulfate detection method | |
| CN107400240A (en) | A kind of solid phase extraction concentration material for bisphenol-A detection and its preparation method and application | |
| CN103293260A (en) | Method for high-efficiency detection of rhodamine B in food and rapid detection kit | |
| CN112924595B (en) | Method for detecting acrylic resin monomer migration volume in paper packaging material | |
| CN107860858A (en) | A kind of method for high-flux analysis of mycotoxin in plant medicine material | |
| CN102798656B (en) | Method for separating 3-hydroxyl glutaric acid monoester enantiomer by high-performance capillary electrophoresis | |
| CN1861629A (en) | Process for pretreating vancomycin ferment fluid for HPLC analysis | |
| Dabek‐Zlotorzynska et al. | Capillary electrophoresis determinative and GC‐MS confirmatory method for water‐soluble organic acids in airborne particulate matter and vehicle emission | |
| CN1828277A (en) | Leather Cr6+ automatic analysis method | |
| CN102735770B (en) | Quantitative detection method of aroma components in yellow serofluid | |
| CN113049324A (en) | Pretreatment method suitable for aquatic product anesthetic or veterinary drug | |
| Miller et al. | The analysis of EDTA in dried bloodstains by electrospray LC-MS-MS and ion chromatography | |
| CN104459009B (en) | A kind of quick detection kit of melamine and using method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |