CN1923857A - Preparation method of high magnetism content monodisperse hydrophilicity magnetic composite microsphere - Google Patents
Preparation method of high magnetism content monodisperse hydrophilicity magnetic composite microsphere Download PDFInfo
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Abstract
The invention discloses a preparing method of high-magnetic single-dispersing hydrophilic composite micro-ball, which comprises the following steps: adopting co-sediment method to prepare nanometer ferroferric oxide particle decorated by oleic acid; preparing magnetic spherical polymer or micro-ball through fine emulsion-emulsion polymerizing method; utilizing Twin to obtain magnetic particle polymer with high-content single dispersion; adopting gel-sol method to cover magnetic core through silica to obtain the product.
Description
Technical field
The present invention relates to the method in a kind of bioseparation material technology field, specifically is a kind of preparation method of high magnetism content monodisperse hydrophilicity magnetic composite microsphere.
Technical background
In recent years, nanometer supperparamagnetic particles or microballoon are widely used at biological, medical field with its remarkable characteristic.Usually, the magnetic carrier that is applied to bioseparation and purifying field should possess following characteristic: superparamagnetism, particle or microballoon size evenly, have surface functional group, magnetic response characteristic fast (the magnetic substance content of height and homogeneous), do not have magneticsubstance to reveal and excellent biological compatibility etc.
As most important magnetic carrier, the research of magnetic microsphere starts from late 1970s, Ugelstad successfully adopts active swelling method to prepare the uniform monodispersed magnetic polystyrene microsphere of yardstick (commodity dynabeads by name at present), but the microballoon yardstick is a micro-meter scale, when descending the step to use, microballoon must be disintegrated down from biomolecule surface again, thereby influence downstream process, in addition because the restriction of technology itself, magnetic substance content is lower in the microballoon, and the surface of hydrophobic polystyrene makes the magnetic ball easily take place to reunite in the biologic applications system and influences the stability of separating effect.It is another kind of that what obtain the market widespread use is that the class size of dextran bag quilt of Meltenyi company is less than the 50nm magnetic-particle, because size is little, downstream application and operation are had no significant effect, therefore need not be in carrying out the bioseparation purge process from the biomolecule surface magnetic-particle that dissociates, very convenient to use, but this type of material magnetic response degree is very low, needs very strong externally-applied magnetic field to catch it, also limits its application.For the biologic applications system, except possessing high magnetic content, single dispersion and function of surface characteristic, microballoon should be able to keep good dispersion and not reunite under various application conditions.In recent years, discover that adopting silicon oxide to coat magnetic carrier that nano ferriferrous oxide granule obtains is the another kind of comparatively ideal material of polymer microballoon of continuing.
Find through literature search prior art, Younan Xia etc. are in " adopting sol-gel method to modify the Study on Surface of super-paramagnetism nano ferric oxide particles " that " nanometer wall bulletin " the 2nd volume 183-186 page or leaf is delivered, (Modifying the Surface Properties of Superparamagnetic IronOxideNanoparticles through A Sol Gel Approach, Nano letters, 2002, Vol2, No.3,183-186) reported the lipophilicity water-based magnetic fluid EMG 304 that utilizes commerce, behind the magnetic-particle surface amination, utilize sol-gel technique, in isopropanol solvent, be catalyzer with ammoniacal liquor, tetraethoxy (TEOS) is the silicon source, generation hydrolysis of magnetic particle surface and polycondensation at single have obtained monodispersed silicon oxide microsphere, and microspheres prepared can be directed under magnetic field after closing the fluorescein coupling.Though this literary composition has obtained hydrophilic magnetic microsphere, yet that weak point is the microballoon magnetic content that synthesizes is very low, and the nuclear of every magnetic ball is made up of a magnetic particle, thereby the magnetic response degree of microballoon is very low, can't satisfy the biomedical applications demand in downstream.At present, adopt silicon oxide to coat superparamagnetic material and all exist in the microballoon (or material) magnetic-particle content low or be not superparamagnetism or the inhomogenous problem of microballoon granularity, thereby limited their application.
Summary of the invention
The present invention is directed to the problem that exists in the background technology, a kind of preparation method of high magnetism content monodisperse hydrophilicity magnetic composite microsphere is proposed, make it solve above deficiency, guarantee finally to obtain microballoon have good hydrophilicity can in, greatly degree has improved the homogeneity of the content of magnetic substance in the gained hydrophilic nano magnetic microsphere and microballoon size, pattern again.
The present invention is achieved by the following technical solutions, the present invention at first adopts coprecipitation method to prepare the nano ferriferrous oxide granule of oleic acid modified, then prepare monodispersed magnetic-particle spheroidal aggravation of high magnetic content or microballoon, promptly adopt based on miniemulsion-emulsion polymerisation process and obtain high magnetic content monodisperse magnetic microballoon, utilize tween surfactants to obtain the monodispersed magnetic-particle spheroidal aggravation of high magnetic content in nano magnetic particle surface self-assembly, be respectively the base nuclear of wetting ability magnetic microsphere then with above-mentioned magnetic microsphere or magnetic spheroidal aggravation, adopt improved sol-gel process that magnetic base nuclear is carried out the silicon oxide shell again and coat, finally obtain the monodispersed silicon oxide magnetic microsphere of wetting ability high magnetic content (greater than 50wt%).Because the present invention adopts monodispersed magnetic-particle spheroidal aggravation of high magnetic content (magnetic-particle content is greater than 70wt%) or microballoon base nuclear, therefore very big degree has improved total magnetic content of final hydrophilic microballoon, in addition, owing to adopt miniemulsion-emulsion polymerisation process and tensio-active agents such as utilizing tween to obtain the single magnetic microsphere and magnetic spheroidal aggravation that disperses high magnetic content of pattern and yardstick homogeneous in nano magnetic particle surface self-assembly, the uniform morphology and the yardstick of final wetting ability microballoon have been guaranteed, simultaneously because the employing silicon oxide is a shell, the abundant silicon hydroxyl of resulting microsphere surface can be implemented various functionalization easily by sophisticated chemistry of silicones means, so the present invention is from truly having realized the collection homogeneous grain diameter first, consistent appearance, high magnetic content, the preparation of surface-functionalized wetting ability magnetic microsphere.
Below method of the present invention is done further to limit, concrete steps are as follows:
(1) adopt coprecipitation method to prepare the nano ferriferrous oxide granule of oleic acid modified.Under the state that keeps logical nitrogen under 80 ℃ to containing Fe
2+And Fe
3+Add excess of ammonia water in the aqueous solution of salt, insulation reaction accounts for Fe to the reaction system adding after 0.5 hour again
3O
4Weight ratio is a 20%-50% oleic acid, behind the lasting insulation reaction 1h, opens wide reaction system with the excessive ammonia evaporation fully, can obtain the nanometer Fe of oleic acid bag quilt
3O
4Particle.With behind the deionized water thorough washing be neutral until supernatant and the action of a magnetic field under abandoning supernatant, use absolute ethanol washing three times again after, 50 ℃ of vacuum-dryings obtain nanometer Fe
3O
4Particle is distributed to this powder in the octane of metering, obtains the oleic acid bag by nanometer Fe
3O
4Particulate octane dispersion liquid, nanometer Fe in the dispersion liquid
3O
4Granule content is 40-70wt%.
(2) adopt miniemulsion-emulsion polymerisation process to obtain the monodispersed magnetic microsphere of high magnetic content, and utilize tween surfactants in nanometer Fe
3O
4The particle surface self-assembly obtains the monodispersed magnetic-particle spheroidal aggravation of high magnetic content.
Described employing miniemulsion-emulsion polymerisation process obtains the monodispersed magnetic microsphere of high magnetic content, and is specific as follows: prepare two kinds of different miniemulsion systems at first respectively: the nanometer Fe that previous step is obtained
3O
4Octane dispersion liquid and water and tensio-active agent mix by metering, ultrasonic back formation o/w type miniemulsion A, and the ultrasonic power variable range is 200-800W, is used for controlling the particle diameter of the disperse phase oil droplet that contains inorganic nanoparticles of formation.Another miniemulsion B adopts 300r/min to stir or the template emulsor adds vinylbenzene the miniemulsion system that emulsification in the aqueous solution of sodium lauryl sulphate of the 0.1wt% for preparing in advance forms the micro-meter scale of uniform particle diameter, and miniemulsion A and miniemulsion B all must not have the existence of hungry area bundle by guarantee system.Then two kinds of miniemulsions are mixed, add water soluble starter, logical nitrogen stirred after 0.5 hour, placed 60-80 ℃ of water-bath reaction to obtain single nanometer magnetic composite microspheres that disperses high magnetic content more than 15 hours, microsphere diameter 60-200nm is controllable, nanometer Fe in the microballoon
3O
4Content is greater than 70wt%.At last, the above-mentioned magnetic microsphere that obtains being distributed to concentration is 0.5
In the polysorbas20 aqueous solution of wt%, the suspensoid that obtains concentration and be 1wt% is standby.
The described tween surfactants of utilizing is in nanometer Fe
3O
4The particle surface self-assembly obtains the monodispersed magnetic-particle spheroidal aggravation of high magnetic content, and is specific as follows: at first with the above-mentioned nanometer Fe that obtains
3O
4The octane dispersion liquid joins in the polysorbas20 aqueous solution that concentration is 0.4-1.0%, wherein nanometer Fe
3O
4Content be 0.1wt%-0.03wt%, obtain the emulsion of black behind the 50-200W supersound process 10min, will take advantage of the beaker of emulsion to place magneticstrength then is special magnetic field one sides of 400 millis, treats nanometer Fe
3O
4Aggregate is adsorbed on the rear flank near magnetic field fully, discard the stillness of night of not caught by magnetic field, and then in beaker, add the polysorbas20 aqueous solution that concentration is 0.4-1.0% again, ultrasonic, magnetic field treatment, ultrasonic, magnetic field treatment again, more than 3 times, obtain the nanometer Fe of stable dispersion in the polysorbas20 aqueous solution so repeatedly
3O
4Particulate conglobulation liquid suspension finally keeps nanometer Fe in the suspension
3O
4Concentration is 1wt%.The aggregate median size is 30-100nm, and size can be by the concentration of polysorbas20 and the size regulation and control of ultrasonic power.
(3) serve as base nuclear with monodispersed magnetic microsphere of the above-mentioned high magnetic content for preparing or magnetic-particle spheroidal aggravation, adopt improved sol-gel process, magnetic base nuclear is carried out the silicon oxide shell coat, finally obtain the monodispersed silicon oxide magnetic microsphere of wetting ability high magnetic content.Mainly be to realize by the following method: with organic alcohol is solvent, ammoniacal liquor is catalyzer, tetraethoxy is for forming the silicon source of silicon oxide shell, monodispersed magnetic microsphere of high magnetic content or magnetic-particle aggregate are base nuclear (hereinafter to be referred as magnetic base nuclear aqeous suspension) to be coated, tetraethoxy with metering fully is dissolved in organic alcohol earlier, the magnetic base nuclear aqeous suspension that will be pre-mixed metering ammoniacal liquor again added reaction system after room temperature reaction 10-48 hour, again with the product dehydrated alcohol that obtains, deionized water wash three times and after 50 ℃ of vacuum-drying finally obtains high magnetism content monodisperse hydrophilicity magnetic composite microsphere.Wherein organic alcohol: ammoniacal liquor: tetraethoxy: the volume ratio between monodispersed magnetic microsphere of high magnetic content or the magnetic-particle aggregate aqeous suspension is 20: 10: 0.2: 10 to 20: 0.1: 0.05: 1.0.
Preparation nanometer Fe of the present invention
3O
4Particulate Fe
3+Salt is iron trichloride, Fe
2+Salt is a kind of in iron protochloride or the ferrous sulfate.
Miniemulsion-emulsion polymerisation process of the present invention obtains the difference that there is at least one order of magnitude in the disperse phase droplet diameter of previously prepared miniemulsion A and miniemulsion B two individual system among the monodispersed magnetic microsphere preparation method of high magnetic content, and quantity differs 10
4More than.The disperse phase droplet diameter scope that contains inorganic particle in the miniemulsion A system is at 50-180nm, and in the miniemulsion B system disperse phase monomer droplet diameter greater than 1 μ m.The film emulsifier unit that described template emulsor is SPG.
It is a kind of in Virahol, the ethanol that the improved sol-gel process of employing of the present invention carries out organic alcohol in the silicon oxide shell coating preparation method to magnetic base nuclear.
The present invention is directed to prepare in the past and have the low and microspherulite diameter skewness of magnetic substance content in the Superparamagnetism composite microsphere and problem such as reunion easily takes place in water, a kind of preparation method of preparation high magnetism content monodisperse hydrophilicity magnetic composite microsphere of novelty has been proposed, owing at first adopt miniemulsion-emulsion polymerisation process and utilize tensio-active agent such as tween in nanometer Fe
3O
4The particle surface self-assembly obtains the monodispersed magnetic microsphere of high magnetic content and two innovative technologies of magnetic-particle spheroidal aggravation, and be magnetic base nuclear with them, silicon oxide shell thickness and condition of surface by improved sol-gel technique control microsphere surface, guarantee finally to obtain microballoon have good hydrophilicity can in, greatly degree has improved the homogeneity of the content of magnetic substance in the gained hydrophilic nano magnetic microsphere and microballoon size, pattern again.Therefore overcome the situation that only contains a small amount of several nano magnetic particles in the silicon oxide magnetic microsphere of correlation technique fully, operation is simple and feasible in the present invention, the controllable thickness of the shell of silicon oxide, thereby be a kind of biomagnetism isolated vectors material that is suitable as very much.
Description of drawings
Fig. 1 prepares the high magnetism content monodisperse hydrophilicity magnetic composite microsphere design sketch of gained for the present invention
Embodiment
Provide following examples in conjunction with technical solution of the present invention:
Embodiment 1 adopts miniemulsion-emulsion polymerisation process to obtain the monodispersed magnetic microsphere of high magnetic content and examines preparation high magnetism content monodisperse hydrophilicity magnetic microballoon for base
1, Fe
3O
4The preparation of magnetic nano-particle, finishing and Fe
3O
4The preparation of octane dispersion liquid
24gFe
3Cl7H
2O and 18gFe
2SO
47H
2O is dissolved in the 100ml deionized water, fully pours there-necked flask into after the dissolving, and places 80 ℃ of water-baths, and inflated with nitrogen after 200rpm stirs half an hour, will begin reaction in the 50 gram ammoniacal liquor adding reaction systems.Add 3.5g oleic acid after half an hour.Opening reactive system after one hour all is evaporated ammoniacal liquor and afterwards finishes reaction.After magnetic field auxiliary is down with the deionized water thorough washing, be neutrality until supernatant, use absolute ethanol washing three times again after, 50 ℃ of vacuum-dryings obtain nanometer Fe
3O
4Particle powder is distributed to this powder in the octane, obtains the oleic acid bag by nanometer Fe
3O
4Particulate octane dispersion liquid, nanometer Fe in the dispersion liquid
3O
4Granule content is 70wt%.
2, miniemulsion-emulsion polymerisation process prepares the monodispersed magnetic microsphere base nuclear of high magnetic content
At first with the 0.04g sodium lauryl sulphate, 48g deionized water, 2g magnetic content are 70% Fe
3O
4The octane dispersion liquid obtains the miniemulsion A that median size is 95.2nm through 600 watts after ultrasonic 30 minutes.Secondly, vinylbenzene 5g is added in the film emulsifier unit of SPG, clamp-on during pressure 0.048Mpa in the external phase that contains SDS 0.10g and deionized water 80g, the monomer that obtains median size 4.1 μ m disperses miniemulsion B.At last the thin newborn A of magnetic, miniemulsion B are mixed the back and add the 20mg initiator potassium persulfate, keep under the logical nitrogen situation 200rpm mechanical stirring after half an hour reactor to be inserted in 80 ℃ of water-baths polymerization 15 hours, obtain the compound magnetic microsphere of dispersed nano of median size 100.4nm.
3, the preparation of high magnetism content monodisperse hydrophilicity magnetic composite microsphere
At first, the median size that step 2 is obtained is that to be distributed to concentration be in 0.5% the polysorbas20 aqueous solution to the compound magnetic microsphere of dispersed nano of 100.4nm, finally obtaining solid content is the magnetic microsphere suspensoid of 1wt%, get above-mentioned suspensoid 10ml, the ammoniacal liquor that adds 0.5ml 25% mixes and obtains microsphere suspension C, secondly, the 0.16ml tetraethoxy fully is dissolved in the 20ml Virahol, again microsphere suspension C is added wherein, behind the room temperature reaction 24 hours, again with the product dehydrated alcohol that obtains, behind the deionized water wash three times, obtain the high magnetism content monodisperse hydrophilicity magnetic composite microsphere that median size is 140nm after 50 ℃ of dryings of vacuum.As shown in Figure 1.
Embodiment 2 utilizes tensio-active agents such as tween in nanometer Fe
3O
4The particle surface self-assembly obtains the monodispersed magnetic-particle spheroidal aggravation of high magnetic content and is base nuclear preparation high magnetism content monodisperse hydrophilicity magnetic microballoon
1, Fe
3O
4The preparation of magnetic nano-particle, finishing and Fe
3O
4The preparation of octane dispersion liquid
Fe
3O
4The preparation of magnetic nano-particle is with described in the embodiment 1-1.Different is that the oleic acid consumption is 2 grams, is made into the Fe that magnetic content is 50wt% at last
3O
4The octane dispersion liquid.
2, utilize tensio-active agents such as tween in nanometer Fe
3O
4The particle surface self-assembly obtains the monodispersed magnetic-particle spheroidal aggravation of high magnetic content base nuclear
At first with above-mentioned 5 nanometer Fe that restrain that obtain
3O
4It is in 0.4% the polysorbas20 aqueous solution that the octane dispersion liquid joins 150ml concentration, obtains the emulsion of black behind the 50W supersound process 10min, and will take advantage of the beaker of emulsion to place magneticstrength then is special magnetic field one sides of 400 millis, leaves standstill nanometer Fe behind the 10min
3O
4Aggregate is adsorbed on the side near magnetic field fully, discard the stillness of night, and then to add 150ml concentration in beaker again be 0.4% the polysorbas20 aqueous solution, ultrasonic, magnetic field treatment, ultrasonic, magnetic field treatment again, 3 times so repeatedly, finally obtain the nanometer Fe of stable dispersion at aqueous phase
3O
4Particulate conglobulation liquid suspension, suspension concentration is adjusted into 1wt%.The aggregate median size is 98nm.
3, the preparation of high magnetism content monodisperse hydrophilicity magnetic composite microsphere
The preparation method of high magnetism content monodisperse hydrophilicity magnetic composite microsphere is as described in the embodiment 1-3, different is that magnetic base nuclear suspension is the monodispersed magnetic-particle spheroidal aggravation of high magnetic content that above-mentioned steps 2 obtains, the consumption of used tetraethoxy is 0.13ml, ammonia volume is 0.4ml, reaction times is 10 hours, finally obtains the high magnetism content monodisperse hydrophilicity magnetic composite microsphere that mean particle size is 120nm.
Embodiment 3
Nanometer Fe 3O4 preparation method of granules is as described in the embodiment 1, and different is the preparation nanometer Fe
3O
4Used Fe
2+Be iron protochloride, and consumption be 10 the gram, used oleic acid be 5 the gram, the magnetic content of preparation is the Fe of 60wt%
3O
4The octane dispersion liquid.
The preparation method that the high magnetic content list disperses magnetic base nuclear is as described in the embodiment 1, different is adopts that 200W is ultrasonic to prepare the miniemulsion A that median size is 176nm, adopt 300rpm to stir after 0.5 hour and obtain the miniemulsion B that median size is 6000nm, polymeric reaction temperature is 60 ℃, and the high magnetic content list that obtains mean diameter and be 198nm disperses compound magnetic microsphere.
The preparation of high magnetism content monodisperse hydrophilicity magnetic composite microsphere is as described in the embodiment 1, different tetraethoxy consumptions be 0.12ml, ammonia volume is 0.35ml, room temperature reaction 48 hours, and finally obtaining median size is the single high magnetic content hydrophilicity magnetic composite microsphere that disperses of 230nm.
Embodiment 4
The preparation of high magnetism content monodisperse hydrophilicity magnetic composite microsphere is as described in the embodiment 1, the polymeric reaction temperature that different is adopts miniemulsion-emulsion polymerisation process to prepare the monodispersed magnetic microsphere base nuclear of high magnetic content is 70 ℃, carry out silicon oxide shell bag by the time used organic alcohol be that ethanol finally obtains single high magnetic content hydrophilicity magnetic composite microsphere that disperses that median size is 141nm.
Embodiment 5
The preparation of high magnetism content monodisperse hydrophilicity magnetic composite microsphere is as described in the embodiment 2, and different is to utilize tensio-active agents such as tween in nanometer Fe
3O
4The nanometer Fe of 5 grams that the particle surface self-assembly will obtain when obtaining the preparation process of the monodispersed magnetic-particle spheroidal aggravation of high magnetic content base nuclear
3O
4It is in 0.7% the polysorbas20 aqueous solution that the octane dispersion liquid joins 100ml concentration, and adopts that 100W is ultrasonic to obtain the monodispersed magnetic-particle spheroidal aggravation of the high magnetic content base nuclear that particle diameter is 60nm.Used spheroidal aggravation base nuclear is 1ml when adopting sol-gel to prepare high magnetism content monodisperse hydrophilicity magnetic composite microsphere simultaneously, tetraethoxy is 0.05ml, ammoniacal liquor is 0.1ml, reaction times is 48 hours, finally obtains the high magnetism content monodisperse hydrophilicity magnetic composite microsphere that mean particle size is 100nm.
Embodiment 6
The preparation of high magnetism content monodisperse hydrophilicity magnetic composite microsphere is as described in the embodiment 2, and different is to utilize tensio-active agents such as tween in nanometer Fe
3O
4The nanometer Fe of 5 grams that the particle surface self-assembly will obtain when obtaining the preparation process of the monodispersed magnetic-particle spheroidal aggravation of high magnetic content base nuclear
3O
4It is in 1.0% the polysorbas20 aqueous solution that the octane dispersion liquid joins 50ml concentration, and adopts that 200W is ultrasonic to obtain the monodispersed magnetic-particle spheroidal aggravation of the high magnetic content base nuclear that particle diameter is 32nm.Used spheroidal aggravation base nuclear is 5ml when adopting sol-gel to prepare high magnetism content monodisperse hydrophilicity magnetic composite microsphere simultaneously, tetraethoxy is 0.15ml, ammoniacal liquor is 10ml, reaction times is 48 hours, finally obtains the high magnetism content monodisperse hydrophilicity magnetic composite microsphere that mean particle size is 60nm.
Claims (8)
1, a kind of preparation method of high magnetism content monodisperse hydrophilicity magnetic composite microsphere, it is characterized in that, at first adopt coprecipitation method to prepare the nano ferriferrous oxide granule of oleic acid modified, then prepare monodispersed magnetic-particle spheroidal aggravation of high magnetic content or microballoon, promptly adopt based on miniemulsion-emulsion polymerisation process and obtain high magnetic content monodisperse magnetic microballoon, utilize tween to obtain the monodispersed magnetic-particle spheroidal aggravation of high magnetic content in nano magnetic particle surface self-assembly, be respectively the base nuclear of wetting ability magnetic microsphere then with above-mentioned two kinds of magnetic microspheres or magnetic spheroidal aggravation, adopt sol-gel process that magnetic base nuclear is carried out the silicon oxide shell again and coat, finally obtain the silicon oxide magnetic microsphere of monodisperse hydrophilicity high magnetic content.
2, the method for preparing high magnetism content monodisperse hydrophilicity magnetic composite microsphere according to claim 1 is characterized in that, described employing coprecipitation method prepares the nano ferriferrous oxide granule of oleic acid modified, and tool is as follows:
Under the state that keeps logical nitrogen under 80 ℃ to containing Fe
2+And Fe
3+Add excess of ammonia water in the aqueous solution of salt, insulation reaction accounts for Fe to the reaction system adding after 0.5 hour again
3O
4Weight ratio is a 20%-50% oleic acid, behind the lasting insulation reaction 1h, opens wide reaction system with the excessive ammonia evaporation fully, promptly obtains the nanometer Fe of oleic acid bag quilt
3O
4Particle, with behind the deionized water thorough washing until supernatant be neutral and under the action of a magnetic field abandoning supernatant, use absolute ethanol washing three times again after, after 50 ℃ of vacuum-drying, obtain nanometer Fe
3O
4Particle is distributed to this powder in the octane of metering, obtains the oleic acid bag by nanometer Fe
3O
4Particulate octane dispersion liquid, nanometer Fe in the dispersion liquid
3O
4Granule content is 40-70wt%.
3, according to the preparation method of claim 1 or 2 described preparation high magnetism content monodisperse hydrophilicity magnetic composite microspheres, it is characterized in that described preparation nanometer Fe
3O
4Particulate Fe
3+Salt is iron trichloride, Fe
2+Salt is a kind of in iron protochloride or the ferrous sulfate.
4, the method for preparing high magnetism content monodisperse hydrophilicity magnetic composite microsphere according to claim 1 is characterized in that, employing obtain high magnetic content monodisperse magnetic microballoon based on miniemulsion-emulsion polymerisation process, specific as follows:
With the nanometer Fe that obtains
3O
4Octane dispersion liquid and water and tensio-active agent mix by metering, ultrasonic back formation o/w type miniemulsion A, and the ultrasonic power scope is 200-800W, is used for controlling the particle diameter of the disperse phase oil droplet that contains inorganic nanoparticles of formation; Another miniemulsion B adopts 300r/min to stir or the template emulsor adds vinylbenzene the miniemulsion system that breast in the aqueous solution of sodium lauryl sulphate of the 0.1wt% for preparing in advance forms the micro-meter scale of uniform particle diameter, miniemulsion A and miniemulsion B do not have the hungry area bundle and exist, then two kinds of miniemulsions are mixed, add water soluble starter, logical nitrogen stirred after 0.5 hour, place 60-80 ℃ of water-bath reaction to obtain single nanometer magnetic composite microspheres that disperses high magnetic content more than 15 hours, microsphere diameter 60-200nm, nanometer Fe in the microballoon
3O
4Content is greater than 70wt%; At last, the above-mentioned magnetic microsphere that obtains is distributed in the polysorbas20 aqueous solution that concentration is 0.5wt%, the suspensoid that obtains concentration and be 1wt% is standby.
5, according to the preparation method of claim 1 or 4 described preparation high magnetism content monodisperse hydrophilicity magnetic composite microspheres, it is characterized in that, described miniemulsion-emulsion polymerisation process obtains the difference that there is at least one order of magnitude in the disperse phase droplet diameter of previously prepared miniemulsion A and miniemulsion B two individual system among the monodispersed magnetic microsphere preparation method of high magnetic content, and quantity differs 10
4, the disperse phase droplet diameter scope that contains inorganic particle in the miniemulsion A system is at 60-200nm, and in the miniemulsion B system disperse phase monomer droplet diameter greater than 1 μ m, the film emulsifier unit that described template emulsor is SPG.
6, the method for preparing high magnetism content monodisperse hydrophilicity magnetic composite microsphere according to claim 1 is characterized in that, the described tween surfactants of utilizing is in nanometer Fe
3O
4The particle surface self-assembly obtains the monodispersed magnetic-particle spheroidal aggravation of high magnetic content, and is specific as follows:
At first with the above-mentioned nanometer Fe that obtains
3O
4The octane dispersion liquid joins in the polysorbas20 aqueous solution that concentration is 0.4-1.0%, wherein nanometer Fe
3O
4Content be 0.1wt%-0.03wt%, obtain the emulsion of black behind the 50-200W supersound process 10min, will take advantage of the beaker of emulsion to place magneticstrength then is special magnetic field one sides of 400 millis, treats nanometer Fe
3O
4Aggregate is adsorbed on the rear flank near magnetic field fully, discard the stillness of night of not caught by magnetic field, and then in beaker, add the polysorbas20 aqueous solution that concentration is 0.4-1.0% again, ultrasonic, magnetic field treatment, ultrasonic, magnetic field treatment again, more than 3 times, obtain the nanometer Fe of stable dispersion in the polysorbas20 aqueous solution so repeatedly
3O
4Particulate conglobulation liquid suspension finally keeps nanometer Fe in the suspension
3O
4Concentration is 1wt%, and the aggregate median size is 30-100nm, and size is by the concentration of polysorbas20 and the size regulation and control of ultrasonic power.
7, the method for preparing high magnetism content monodisperse hydrophilicity magnetic composite microsphere according to claim 1, it is characterized in that, described employing sol-gel process, magnetic base nuclear is carried out the silicon oxide shell to coat, finally obtain the monodispersed silicon oxide magnetic microsphere of wetting ability high magnetic content, specific as follows:
With organic alcohol is solvent, ammoniacal liquor is catalyzer, tetraethoxy is for forming the silicon source of silicon oxide shell, monodispersed magnetic microsphere of high magnetic content or magnetic-particle aggregate are base nuclear to be coated, hereinafter to be referred as magnetic base nuclear aqeous suspension, tetraethoxy with metering fully is dissolved in organic alcohol earlier, the magnetic base nuclear aqeous suspension that will be pre-mixed metering ammoniacal liquor again added reaction system after room temperature reaction 10-48 hour, again with the product dehydrated alcohol that obtains, deionized water wash three times and after 50 ℃ of vacuum-drying, finally obtain high magnetism content monodisperse hydrophilicity magnetic composite microsphere, wherein organic alcohol: ammoniacal liquor: tetraethoxy: the volume ratio between monodispersed magnetic microsphere of high magnetic content or the magnetic-particle aggregate aqeous suspension is 20: 10: 0.2: 10 to 20: 0.1: 0.05: 1.0.
8, according to the preparation method of claim 1 or 7 described preparation high magnetism content monodisperse hydrophilicity magnetic composite microspheres, it is characterized in that it is a kind of in Virahol, the ethanol that described employing sol-gel process carries out organic alcohol in the silicon oxide shell coating preparation method to magnetic base nuclear.
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Cited By (13)
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| CN101745353A (en) * | 2010-02-12 | 2010-06-23 | 上海交通大学 | Magnetic microsphere as well as preparation and preparation method thereof used for purifying histidine tag protein |
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