CN1911192A - Apparatus for inducing expression of retina heat shock protein and its application - Google Patents
Apparatus for inducing expression of retina heat shock protein and its application Download PDFInfo
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- CN1911192A CN1911192A CN 200610100943 CN200610100943A CN1911192A CN 1911192 A CN1911192 A CN 1911192A CN 200610100943 CN200610100943 CN 200610100943 CN 200610100943 A CN200610100943 A CN 200610100943A CN 1911192 A CN1911192 A CN 1911192A
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- shock protein
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Abstract
A device able to induce the expression of heat shock protein on retina for treating glaucoma and visual nerve lesion is mainly a near-infrared laser device to emit near-infrared pulses for low-energy thermal stimulation to the retina via slit-lamp microscope, so inducing the expression of heat shock protein.
Description
Technical field
The present invention relates to a kind of device of inducing heat shock protein expression, being specifically related to this device can local physical stimulation eyes retina tissue, can the safe and effective heat shock protein expression of inducing, thus reach the purpose of control glaucomatous optic neuropathy.
Background technology
Glaucoma (Glaucoma) be one group because absoluteness or relative property intraocular pressure constitutional or the Secondary cases optic nerve injury disease due to raising.Such disease has now become the primary irreversible blinding oculopathy in the Asia and even the whole world.Modern glaucoma means of prevention comprises intraocular pressure dependency controlling factors and two aspects of non-intraocular pressure dependency factor treatment.Along with the renewal and the exploitation of operation, laser and novel intraocular pressure lowering medicine, intraocular pressure dependency factor has obtained solution largely.And for the ready-made focus for international ophthalmology educational circles and the research of neuroscience educational circles of the protection of non-intraocular pressure dependency glaucoma optic nerve lesion.Studies confirm that in a large number glaucoma optic nerve lesion mechanism is that retinal ganglial cells (RGC) programmed death is an apoptosis, therefore, how suppressing the RGC apoptosis is the key that reaches protection glaucoma optic nerve function purpose.(heat shock protein is an albuminoid the most conservative in the organism evolutionary process HSP) to heat shock protein, has anti-damage, anti-apoptosis biological function.Confirmed that now HSP is bringing into play the critical function that the neuroprotective cell is avoided secondary damage in multiple nervous system injury and degenerative disease, and himself antibody pass through in and the HSP function, then promoted the neuronal apoptosis generation.The method that existing researcher is used the HSP derivant by whole body reaches the purpose that RGC protects; other organizes normal cell to have potential toxic and side effects but this method is to whole body; therefore; how can when not damaging the normal body cell, promote neurocyte to express endogenous HSP by local excitation; thereby the apoptosis that suppresses retinal ganglial cells becomes optic nerve protection problem anxious to be solved.
Summary of the invention
Can address the above problem by device provided by the present invention.
This device is the near-infrared wavelength laser machine that assembles and transfer with slit lamp microscope, its laser pulse that sends carries out the physics by the eye contact lens and the big pupil that looses to the optical fundus retinal tissue to stimulate, and can induce retinal neuronal cell to express the endogenous heat shock protein safely.
This device is mainly by forming as lower member: (1) sustainable laser machine that sends the near-infrared wavelength laser pulse, and its laser energy is 50mw, pulse width is that irradiation time is 15-30 second; (2) 1.2mm light spot laser slit lamp adapter; (3) slit lamp microscope; (4) slit lamp microscope converting interface; (5) cornea contact optical fundus lens.During with this device irradiation retina, the thermostimulation temperature maintenance of irradiation is about 40 ℃.And described thermostimulation mode repeatability is carried out.
Description of drawings:
Fig. 1 adopts near-infrared wavelength laser, and through 1.2mm light spot laser slit lamp adapter, after the slit lamp microscope switching, big pupil shines BN rat optical fundus retinal tissue by loosing
Fig. 2 adopts near-infrared wavelength laser energy 50mw, and irradiation time 30s, BN rat optical fundus retina irradiation place hot spot are the light grey threshold responses (arrow indication) that is
Fig. 3 adopts near-infrared wavelength laser energy 50mw, irradiation time 20s, and retina irradiation place hot spot in BN rat optical fundus is subthreshold response, does not see obvious morphological change
Fig. 4 near-infrared wavelength laser, energy 50mw, irradiation time 20s, shine back 3 days retina HSP70 immunohistochemical stainings, visible retinal neuronal cell endochylema HSP70 strongly expressed (brown, DAB colour developing), normal retina only has weak positive staining, and negative control group does not have positive dyeing, * 40
Fig. 5 near-infrared wavelength laser, energy 50mw, irradiation time 20s, shine back 3 days retina HSP27 immunohistochemical stainings, visible retinal neuronal cell endochylema HSP27 strongly expressed (brown, DAB colour developing), normal and negative control group does not all have positive dyeing, * 40
Fig. 6 near-infrared wavelength laser, energy 50mw, irradiation time 20s, the irradiation retina after 3 days, acute rising intraocular pressure adopts TUNEL colouring method labelling and counts RGC layer apoptotic cell, can reduce the apoptosis of RGC under the acute high intraocular pressure situation after the intervention of near-infrared wavelength laser.(1) high intraocular pressure group, the high intraocular pressure group that (2) TTT intervenes, (3) TTT group, (4) normal group (sword fingers apoptotic cell), * 40
The concrete operations mode
Device of the present invention by the contact optical fundus lens and the big pupil that looses, is focused on retina with laser aiming light.Starting laser shines desiring the irradiated site retina.Its irradiation reaction is subthreshold response, and we experimental results show that the expression that can induce the most relevant heat-shock protein family-HSP27 of RGC apoptosis and HSP70, has reduced the apoptosis of RGC under the high intraocular pressure situation of BN pigment rat acute.
Claims (3)
1, a kind of device of inducing glaucoma patient pathological changes retinal neuronal cell heat shock protein expression, it is characterized in that this device is mainly by forming as lower member: (1) sustainable laser machine that sends the near-infrared wavelength laser pulse, its laser energy is 50mw, and pulse width is that irradiation time is 15-30 second; (2) 1.2mm light spot laser slit lamp adapter; (3) slit lamp microscope; (4) slit lamp microscope converting interface; (5) cornea contact optical fundus lens.
2, device according to claim 1, when it is characterized in that with this device irradiation retina, the thermostimulation temperature maintenance of irradiation is about 40 ℃.
3, device according to claim 2 is characterized in that wherein said thermostimulation mode repeatability carries out.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200610100943A CN1911192B (en) | 2006-07-29 | 2006-07-29 | Apparatus for inducing expression of retina heat shock protein and its application |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200610100943A CN1911192B (en) | 2006-07-29 | 2006-07-29 | Apparatus for inducing expression of retina heat shock protein and its application |
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| Publication Number | Publication Date |
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| CN1911192A true CN1911192A (en) | 2007-02-14 |
| CN1911192B CN1911192B (en) | 2010-05-12 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN200610100943A Expired - Fee Related CN1911192B (en) | 2006-07-29 | 2006-07-29 | Apparatus for inducing expression of retina heat shock protein and its application |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109562272A (en) * | 2016-08-09 | 2019-04-02 | 奥哈伊视网膜技术有限责任公司 | For providing the method that protectiveness is treated to biological tissue or fluid |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3206923B2 (en) * | 1991-01-30 | 2001-09-10 | 株式会社ニデック | Ophthalmic laser surgery device |
| US6631990B2 (en) * | 2001-01-03 | 2003-10-14 | Acmed, Inc. | System for measuring distances in the anterior chamber of the eye |
| JP3750935B2 (en) * | 2002-03-26 | 2006-03-01 | 株式会社トプコン | Ophthalmic equipment |
| US20040116909A1 (en) * | 2002-12-11 | 2004-06-17 | Ceramoptec Industries Inc. | Multipurpose diode laser system for ophthalmic laser treatments |
| JP2005046247A (en) * | 2003-07-31 | 2005-02-24 | Topcon Corp | Laser surgery apparatus |
| CN1682671A (en) * | 2004-04-13 | 2005-10-19 | 首都医科大学附属北京同仁医院 | Expression of pathologic change retinal nerve cell heat shock protein |
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- 2006-07-29 CN CN200610100943A patent/CN1911192B/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109562272A (en) * | 2016-08-09 | 2019-04-02 | 奥哈伊视网膜技术有限责任公司 | For providing the method that protectiveness is treated to biological tissue or fluid |
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| Publication number | Publication date |
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| CN1911192B (en) | 2010-05-12 |
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