CN1904072B - Gene composition for screening pancreatic catheter gland cancer and its application - Google Patents

Gene composition for screening pancreatic catheter gland cancer and its application Download PDF

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CN1904072B
CN1904072B CN2006100526794A CN200610052679A CN1904072B CN 1904072 B CN1904072 B CN 1904072B CN 2006100526794 A CN2006100526794 A CN 2006100526794A CN 200610052679 A CN200610052679 A CN 200610052679A CN 1904072 B CN1904072 B CN 1904072B
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sample
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pbmc
carcinoma
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CN1904072A (en
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吴育连
燕海潮
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Zhejiang University ZJU
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Zhejiang University ZJU
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Abstract

The present invention provides a group of assortment of genes, including 10 genes; L48784, X83300, A1620827, A1494291, NM-020970, BF433657, JO2959, BC 022313, NM-000904 and NM-004994, for screening ductal adenocarcinoma of pancreas. Said invention can utilize detection of said group of genes of human peripheral blood mononuclear cell to screen out patient with pancreatic cancer from health human group.

Description

The assortment of genes and the application of one group of screening ductal adenocarcinoma of pancreas
Technology neck city
The invention belongs to biotechnology, relate generally to one group of assortment of genes, be used to screen ductal adenocarcinoma of pancreas.
Background technology
Carcinoma of the pancreas is the very poor malignant tumour of prognosis.Overall median survival time was less than 6 months.Survival rate had only 0.4-5% in 5 years.The surgical excision tumour can improve lifetime, and the patient's of ocal resection median survival time can reach 11-20 month, and survival rate can reach 7-25% in 5 years.But because the carcinoma of the pancreas patient lacks early stage clinical manifestation.When going to a doctor, patient how to have belonged to late period.
Because the operation risk of carcinoma of the pancreas excision and biopsy is bigger, thus carcinoma of the pancreas clinical samples and be not easy to obtain, this just make most of carcinoma of the pancreas patient can not get clear and definite with diagnose timely, thereby incured loss through delay patient's treatment.Therefore, if can from carcinoma of the pancreas patient's PBMC, find the relevant gene of tumour, thereby reach the purpose of diagnosing tumour, with the clinical position that instructs us well.
At present, CA199 is the pancreatic tumour mark that the most often uses.Research about CA199 is a lot of.Most research results thinks that the susceptibility of CA199 is about 80%, and specificity is present getable best pancreatic tumour mark about 60-70%.CA199 raises also can occur in other malignants tumor of digestive tract, like cancer of the stomach, colorectal carcinoma, biliary system malignant tumour; Also can occur in benign lesion, like pancreatitis, hepatitis and liver cirrhosis etc.
At present also do not have ideal to come the pancreatic tumour mark of autoblood, seek the tumor markers in the better carcinoma of the pancreas patient blood, have very high clinical value.
Summary of the invention
The purpose of this invention is to provide the assortment of genes of one group of screening ductal adenocarcinoma of pancreas, comprise 10 gene: L48784, X83300, AI620827, AI494291, NM_020970, BF433657, J02959, BC022313, NM_000904, NM_004994.
Another object of the present invention provides the application of this assortment of genes in the screening ductal adenocarcinoma of pancreas.
The present invention can filter out the carcinoma of the pancreas patient through detecting this group gene of human PMNC from healthy population, specifically screen step and be:
1. according to Affymetrix chip gene expression profile operational guidance; Use AFFYMETRIXHUMAN GENOME U133 PLUS 2.0ARRAY to detect this expression of group gene in 5 carcinoma of the pancreas and 5 normal peoples' PMNC (PBMC), obtain the signal value of genetic expression;
2.5 the average signal value of name carcinoma of the pancreas patient's PMNC (PBMC) is higher than 5 normal peoples' gene and is considered to the up-regulated expression gene; The gene that the average signal value of 5 carcinoma of the pancreas is lower than 5 normal peoples is considered to reduce expressing gene;
3. the median of getting genetic expression signal value in above-mentioned 10 PMNCs is as threshold value, is to raise or downward modulation is expressed with the genetic expression of the PBMC that judges tested person; The people's of tested person PBMC, the expression trend that in 10 genes, has the expression conditions more than 7 or 7 in carcinoma of the pancreas PBMC, to raise or reduce with these genes is consistent, and just infers the artificial carcinoma of the pancreas patient of tested person; If in the people's of tested person PBMC, in 10 genes, the expression trend that has expression conditions and these genes more than 7 or 7 in carcinoma of the pancreas, to raise or reduce does not meet, and the people who just infers tested person is the normal people.
The present invention utilizes the chip gene expression profile of Affymetrix company to carcinoma of the pancreas patient PMNC (PBMC; Peripheral blood mononuclear cells) express spectra and normal people's PMNC express spectra are studied; The tumor-related gene of screening carcinoma of the pancreas is for the clinical diagnosis of carcinoma of the pancreas and screening provide new approach.Proved that in the experiment of small sample this experiment has very high susceptibility and specificity.Usefulness of the present invention is: (1) patients'blood is the sample that obtains easily; (2) screening method of the present invention is a kind of harmless detection method; (3) small sample high throughput oligonucleotide chip of expression spectrum has proved that this assortment of genes has very high supposition accuracy (100%).
Embodiment
The present invention is further described through embodiment.
Embodiment 1
1. according to Affymetrix chip gene expression profile operational guidance; Use AFFYMETRIXHUMAN GENOME U133 PLUS 2.0 ARRAY to detect this expression of group gene in 5 carcinoma of the pancreas and 5 normal peoples' PBMC; Obtain the signal value of genetic expression, (referring to table 1).
Table 1
Sample L48784 X83300 AI620827 AI494291 NM_02097 ?BF433657 ?J02959 BC022313 ?NM_000904 NM_004994
T1 T2 T3 T4 T5 N1 N2 N3 N4 N5 6615.2 11386.6 5944.1 5007.4 6973.1 21113.1 10817.5 13886.1 16847.6 17733?3 340.3 658.7 496.2 453.5 703.5 773 782.5 1022.6 1836.3 1396.6 657.9 730.5 1018.2 890.8 1478.2 2161.5 1772.8 1846.8 2274.3 1949.2 708.1 553.5 698.7 484.8 673.4 919.2 1117.8 1752.5 1455 1110 508.6 963.8 522.8 745.3 642.2 1460.3 1194.4 1253.1 1472.4 1367.2 ?4103.5?2495.1?3439.2?3504.5?2453.1?1374.7?2041.6?1114.9?1231.2?1311.9 7571.2 10854.5 6894.9 10304.2 8012.7 5454.1 3179.9 2481.6 3431.4 2819.9 3893.3 4182.7 3176.2 5365.8 2728.2 1524 1808 1132.9 1102 1757.6 4635.3 1516.9 5390.5 4523.9 2784.2 1137 1649.9 1678.9 1497.5 770.6 10756.1 4475.7 7375.7 4207.6 3860.3 2043.6 2940 1351.5 1300.5 2171.3
Median T mean N difference in means 2 expression trend 11102.057327.817395.03-10067.23 downward modulation 738.25577.9751257.125-679.15 downward modulation 1625.51029.4252057.95-1028.525 downward modulation 813.65602.61309.175-706.575 downward modulation 1079.1 676.54 1349.48-672.94 downward modulations 2247.35 2972.975 1258.175 1714.8 raise 6174.5 9016.575 3546.75 5469.825 raise 2268.1 3863.225 1379.125 2484.1 raise 1664.4 3553.875 1,271 2282.875 raise 3400.15 4979.825 1716.725 3263.1 raise
The sample packet situation:
Carcinoma of the pancreas group: T1, T2, T3, T4, T5; Normal people's group: N1, N2, N3, N4, N5,
Public ID: public number of gene,
The T mean: the MV of tumor sample signal value,
The N mean: the MV of normal control sample signal value,
Difference 2=T mean-N mean differs from 2＞0 and shows gene up-regulated expression in carcinoma of the pancreas PBMC, differs from 2＜0 explanation genes and in carcinoma of the pancreas PBMC, reduces expression.
Meet criterion: the median of getting genetic expression signal value in above-mentioned 10 PMNCs is as threshold value, is to raise or downward modulation is expressed with the genetic expression of the PBMC that judges tested person; The people's of tested person PBMC, the expression trend that in 10 genes, has the expression conditions more than 7 or 7 in carcinoma of the pancreas PBMC, to raise or reduce with these genes is consistent, and just infers the artificial carcinoma of the pancreas patient of tested person; If in the people's of tested person PBMC, in 10 genes, the expression trend that has expression conditions and these genes more than 7 or 7 in carcinoma of the pancreas, to raise or reduce does not meet, and the people who just infers tested person is the normal people.
2.5 the average signal value of name carcinoma of the pancreas is higher than 5 normal peoples' gene and is considered to the up-regulated expression gene; The gene that the average signal value of 5 carcinoma of the pancreas is lower than 5 normal peoples is considered to reduce expressing gene.Can find out that according to last table L48784, X83300, AI620827, AI49429, NM_020970 are the downward modulation expressing genes; And BF433657, J02959, BC022313, NM_000904, NM_004994 are the up-regulated expression genes.
3. the median of getting gene expression amount detected result among above-mentioned 10 PBMC is as threshold value, is to raise or downward modulation is expressed with the genetic expression of the PBMC that judges tested person;
4. according to Affymetrix chip gene expression profile operational guidance; Use AFFYMETRIXHUMAN GENOME U133 PLUS 2.0 ARRAY to detect this expression of group gene in the people's of tested person PBMC; Screen with above-mentioned screening criteria: the people's of tested person PBMC; The expression trend that in 10 genes, has the expression conditions more than 7 or 7 in carcinoma of the pancreas PBMC, to raise or reduce with these genes is consistent, and just infers the artificial carcinoma of the pancreas patient of tested person; If the people's of tested person PBMC, the expression trend that in 10 genes, has expression conditions and these genes more than 7 or 7 in carcinoma of the pancreas, to raise or reduce does not meet, and just infers the artificial healthy population of tested person;
The present invention infers the diagnostic value (referring to embodiment 2-51) of the assortment of genes of 10 genomic constitutions of this group through k times of cross validation
K times of cross validation is that sample is divided into test group and training group, sets up model through training group sample and comes the test group sample is inferred.After repeating k time, calculate the average accuracy of inferring accuracy for k time again, promptly get the last accuracy of this model.
According to whole 25 kinds of possible permutation and combination, this experiment is divided into 25 groups with the mode of 25 groups of cross validations with sample, and every group has 8 samples (4 cancer and 4 normal) to work as learning sample, and all the other 2 is test sample book.Set up screening criteria (seeing table 2-51) with training group sample, infer whether the test group sample is the accuracy of cancer.
The implication of various abbreviations in table 2-51:
The signal value of the tested sample-median of difference 1=,
The T mean: the MV of tumor sample signal value,
The N mean: the MV of normal control sample signal value,
Difference 2=T mean-N mean differs from 2＞0 and shows gene up-regulated expression in carcinoma of the pancreas PBMC, and differ from 2＜0 explanation genes and in carcinoma of the pancreas PBMC, reduce expression,
Median: the median of the signal value of gene expression amount among 8 PBMC of training group.
" meet " criterion: the median of getting gene expression amount detected result among 8 PBMC of training group is as threshold value, is to raise or downward modulation is expressed with the genetic expression of the PBMC that judges tested person; In test group, the people's of tested person PBMC, the expression trend that in 10 genes, has the expression conditions more than 7 or 7 in carcinoma of the pancreas PBMC, to raise or reduce with these genes is consistent, and just infers the artificial carcinoma of the pancreas patient of tested person; If the people's of tested person PBMC, the expression trend that in 10 genes, has expression conditions and these genes more than 7 or 7 in carcinoma of the pancreas, to raise or reduce does not meet, and just infers the artificial healthy population of tested person; (in brief, differ from 1 with differ from 2 be all negative or be all positive number, then judge to meet; Otherwise, be judged to be and do not meet.)
Embodiment 2
Operation is with reference to embodiment 1, and the result is referring to table 2.
Table 2. cross validation 1-1
Sample L48784 X83300 AI620827 AI494291 NM_020970 BF433657 J02959 BC022313 NM_000904 NM_004994
The training group T2 T3 11386.6 5944.1 658.7 496.2 730.5 1018.2 553.5 698.7 963.8 522.8 2495.1 3439.2 ?10854.5?6894.9 ?4182.7?3176.2 ?1516.9?5390.5 ?4475.7?7375.7
Whether median test group difference 1T mean N difference in means 2 meets T4 T5 N2 N3 N4 N5 T1 5007.4 6973.1 10817.5 13886.1 16847.6 17733.3 11102.05 6615.2-the 4486.85 7327.8 14821.13-7493.33rd, 453.5 703.5 782.5 1022.6 1836.3 1396.6 743 340.3-402.7 577.975 1259.5 681.525 are 890.8 1478.2 1772.8 1846.8 2274.3 1949.2 1625.5 657.9-967.6 1029.425 1960.775-931.35th, 484.8 673.4 1117.8 1752.5 1,455 1,110 904.35 708.1-the 196.25 602.6 1358.825-756.225th, 745.3 642.2 1194.4 1253.1 1472.4 1367.2 1079.1 508.6-570.5 718.525 1321.775-603.25th, 3504.5 2453.1 2041.6 1114.9 1231.2 1311.9 2247.35 4103.5 1856.15 2972.975 1424.9 1548.075 are 10304.2 8012.7 3179.9 2481.6 3431.4 2819.9 5163.15 7571.2 2408.05 9016.575 2978.2 6038.375 are 5365.8 2728.2 1,808 1132.9 1,102 1757.6 2268.1 3893.3 1625.2 3863.225 1450.125 2413.1 are 4523.9 2784.2 1649.9 1678.9 1497.5 770.6 1664.4 4635.3 2970.9 3553.875 1399.225 2154.65 are 4207.6 3860.3 2,940 1351.5 1300.5 2171.3 3400.15 10756.1 7355.95 4979.825 1940.825 3039 are
In the table 2: coincidence rate is 10/10.Infer that tested sample is a carcinoma of the pancreas.
Embodiment 3
Operation is with reference to embodiment 1, and the result is referring to table 3.
Table 3 cross validation 1-2
Sample L48784 X83300 ?AI620827 AI494291 NM_020970 BF433657 J02959 ?BC022313 NM_000904 NM_004994
Whether training group median test group difference 1T mean N difference in means 2 meets ?T2?T3?T4?T5?N2?N3?N4?N5?N1 11386.6 5944.1 5007.4 6973.1 10817.5 13886.1 16847.6 17733.3 11102.05 21113.1 10011.05 7327.8 14821.13-7493.33 are not 658.7 496.2 453.5 703.5 782.5 1022.6 1836.3 1396.6 743 773 30 577.975 1259.5-681.525 are not 730.5 1018.2 890.8 1478.2 1772.8 1846.8 2274.3 1949.2 1625.5 2161.5 536 1029.425 1960.775-931.35 are not 553.5 698.7 484.8 673.4 1117.8 1752.5 1,455 1,110 904.35 919.2 14.85 602.6 1358.825-756.225 are not 963.8 522.8 745.3 642.2 1194.4 1253.1 1472.4 1367.2 1079.1 1460.3 381.2 718.525 1321.775-603.25 are not 2495.1 3439.2 3504.5 2453.1 2041.6 1114.9 1231.2 1311.9 2247.35 1374.7-872.65 2972.975 1424.9 1548.075 are not 10854.5 6894.9 10304.2 8012.7 3179.9 2481.6 3431.4 2819.9 5163.15 5454.1 290.95 9016.575 2978.2 6038.375 are 4182.7 3176.2 5365.8 2728.2 1,808 1132.9 1,102 1757.6 2268.1 1524-744.1 3863.225 1450.125 2413.1 are not 1516.9 5390.5 4523.9 2784.2 1649.9 1678.9 1497.5 770.6 1664.4 1137-527.4 3553.875 1399.225 2154.65 are not 4475.7 7375.7 4207.6 3860.3 2,940 1351.5 1300.5 2171.3 3400.15 2043.6-1356.55 4979.825 1940.825 3039 are not
In the table 3: the rate of not meeting is 9/10.Infer that tested sample is the normal people.
Embodiment 4
Operation is with reference to embodiment 1, and the result is referring to table 4.
Table 4. cross validation 2-1
Sample L48784 X83300 ?AI620827 AI494291 NM_020970 BF433657 J02959 BC022313 NM_000904 NM_004994
Whether training group median test group difference 1T mean N difference in means 2 meets ?T2?T3?T4?T5?N1?N3?N4?N5?T1 11386.6 5944.1 5007.4 6973.1 21113.1 13886.1 16847.6 17733.3 12636.35 6615.2-the 6021.15 7327.8 17395.03-10067.23rd, 658.7 496.2 453.5 703.5 773 1022.6 1836.3 1396.6 738.25 340.3-the 397.95 577.975 1257.125-679.15th, 730.5 1018.2 890.8 1478.2 2161.5 1846.8 2274.3 1949.2 1662.5 657.9-the 1004.6 1029.425 2057.95-1028.525th, 553.5 698.7 484.8 673.4 919.2 1752.5 1,455 1,110 808.95 708.1-the 100.85 602.6 1309.175-706.575th, 963.8 522.8 745.3 642.2 1460.3 1253.1 1472.4 1367.2 1108.45 508.6-the 599.85 718.525 1388.25-669.725th, 2495.1 3439.2 3504.5 2453.1 1374.7 1114.9 1231.2 1311.9 1913.9 4103.5 2189.6 2972.975 1258.175 1714.8 are 10854.5 6894.9 10304.2 8012.7 5454.1 2,481 6 3431.4 2819.9 6174.5 7571.2 1396.7 9016.575 3546.75 5469.825 are 4182.7 3176.2 5365.8 2728.2 1,524 1132.9 1,102 1757.6 2242.9 3893.3 1650.4 3863.225 1379.125 2484.1 are 1516.9 5390.5 4523.9 2784.2 1,137 1678.9 1497.5 770.6 1597.9 4635.3 3037.4 3553.875 1,271 2282.875 are 4475.7 7375.7 4207.6 3860.3 2043.6 1351.5 1300.5 2171.3 3015.8 10756.1 7740.3 4979.825 1716.725 3263.1 are
In the table 4: coincidence rate is 10/10.Infer that tested sample is a carcinoma of the pancreas.
Embodiment 5
Operation is with reference to embodiment 1, and the result is referring to table 5,
Table 5. cross validation 2-2
Sample L48784 X83300 AI620827 AI494291 NM_020970 ?BF433657 ?J02959 BC022313 NM_000904 NM_004994
Whether training group median test group differs from 1 mean N difference in means 2 and meets ?T2?T3?T4?T5?N1?N3?N4?N5?N2 11386.6 5944.1 5007.4 6973.1 21113.1 13886.1 16847.6 17733.3 12636.35 10817.5-the 1818.85 7327.8 17395.03-10067.23rd, 658.7 496.2 453.5 703.5 773 1022.6 1836.3 1396.6 738.25 782.5 44.25 577.975 1257.125-679.15 are not 730.5 1018.2 890.8 1478.2 2161.5 1846.8 2274.3 1949.2 1662.5 1772.8 110.3 1029.425 2057.95-1028.525 are not 553.5 698.7 484.8 673.4 919.2 1752.5 1,455 1,110 808.95 1117.8 308.85 602.6 1309.175-706.575 are not 963.8 522.8 745.3 642.2 1460.3 1253.1 1472.4 1367.2 1108.45 1194.4 85.95 718.525 1388.25-669.725 are not 2495.1 3439.2 3504.5 2453.1 1374.7 1114.9 1231.2 1311.9 1913.9 2041.6 127.7 2972.975 1258.175 1714.8 are 10854.5 6894.9 10304.2 8012.7 5454.1 2481.6 3431.4 2819.9 6174.5 3179.9-2994.6 9016.575 3546.75 5469.825 are not 4182.7 3176.2 5365.8 2728.2 1,524 1132.9 1,102 1757.6 2242.9 1808-434.9 3863.225 1379.125 2484.1 are not 1516.9 5390.5 4523.9 2784.2 1,137 1678.9 1497.5 770.6 1597.9 1649.9 52 3553.875 1,271 2282.875 are 4475.7 7375.7 4207.6 3860.3 2043.6 1351.5 1300.5 2171.3 3015.8 2940-75.8 4979.825 1716.725 3263.1 are not
In the table 5: the rate of not meeting is 7/10.Infer the PBMC of tested sample for the normal people.
Embodiment 6
Operation is with reference to embodiment 1, and the result is referring to table 6.
Table 6. cross validation 3-1
Sample L48784 ?X83300 ?AI620827 ?AI494291 ?NM_020970 BF433657 J02959 BC022313 NM_000904 NM_004994
The training group T2 T3 T4 T5 11386.6 5944.1 5007.4 6973.1 658.7 496.2 453.5 703.5 730.5 1018.2 890.8 1478.2 553.5 698.7 484.8 673.4 963.8 522.8 745.3 642.2 2495.1 3439.2 3504.5 2453.1 10854.5 6894.9 10304.2 8012.7 4182.7 3176.2 5365.8 2728.2 1516.9 5390.5 4523.9 2784.2 4475.7 7375.7 4207.6 3860.3
Whether median test group difference 1T mean N difference in means 2 meets N1N2N4N5T1 21113.1 10817.5 16847.6 17733.3 11102.05 6615.2-the 4486.85 7327.8 16627.88-9300.075th, 773 782.5 1836.3 1396.6 738.25 340.3-the 397.95 577.975 1197.1-619.125th, 2161.5 1772.8 2274.3 1949.2 1625.5 657.9-967.6 1029.425 2039.45-1010.025th, 919.2 1117.8 1,455 1,110 808.95 708.1-the 100.85 602.6 1150.5-547.9th, 1460.3 1194.4 1472.4 1367.2 1079.1 508.6-570.5 718.525 1373.575-655.05th, 1374.7 2041.6 1231.2 1311.9 2247.35 4103.5 1856.15 2972.975 1489.85 1483.125 are 5454.1 3179.9 3431.4 2819.9 6174.5 7571.2 1396.7 9016.575 3721.325 5295.25 are 1,524 1,808 1,102 1757.6 2268.1 3893.3 1625.2 3863.225 1547.9 2315.325 are 1,137 1649.9 1497.5 770.6 1583.4 4635.3 3051.9 3553.875 1263.75 2290.125 are 2043.6 2,940 1300.5 2171.3 3400.15 10756.1 7355.95 4979.825 2113.85 2865.975 are
In the table 6: coincidence rate is 10/10.Infer that tested sample is carcinoma of the pancreas patient's PBMC.
Embodiment 7
Operation is with reference to embodiment 1, and the result is referring to table 7.
Table 7. cross validation 3-2
Sample L48784 X83300 AI620827 AI494291 NM_020970 BF433657 ?J02959 BC022313 NM_000904 ?NM_004994
Whether training group median test group difference 1T mean N difference in means 2 meets ?T2?T3?T4?T5?N1?N2?N4?N5?N3 11386.6 5944.1 5007.4 6973.1 21113.1 10817.5 16847.6 17733.3 11102.05 13886.1 2784.05 7327.8 16627.88-9300.075 are not 658 7 496.2 453.5 703.5 773 782.5 1836.3 1396.6 738.25 1022.6 284.35 577.975 1197.1-619.125 are not 730.5 1018.2 890.8 1478.2 2161.5 1772.8 2274.3 1949.2 1625.5 1846.8 221.3 1029.425 2039.45-1010.025 are not 553.5 698.7 484.8 673.4 919.2 1117.8 1,455 1,110 808.95 1752.5 943.55 602.6 1150.5-547.9 are not 963.8 522.8 745.3 642.2 1460.3 1194.4 1472.4 1367.2 1079.1 1253.1 174 718.525 1373.575-655.05 are not 2495.1 3439.2 3504.5 2453.1 1374.7 2041.6 1231.2 1311.9 2247.35 1114.9-1132.45 2972.975 1489.85 1483.125 are not 10854.5 6894.9 10304.2 8012.7 5454.1 3179.9 3431.4 2819.9 6174.5 2481.6-3692.9 9016.575 3721.325 5295.25 are not 4182.7 3176.2 5365.8 2728.2 1,524 1,808 1,102 1757.6 2268.1 1132.9-1135.2 3863.225 1547.9 2315.325 are not 1516.9 5390.5 4523.9 2784.2 1,137 1649.9 1497.5 770.6 1583.4 1678.9 95.5 3553.875 1263.75 2290.125 are 4475.7 7375.7 4207.6 3860.3 2043.6 2,940 1300.5 2171.3 3400.15 1351.5-2048.65 4979.825 2113.85 2865.975 are not
In the table 7: coincidence rate is 9/10.Infer the PBMC of tested sample for the normal people.
Embodiment 8
Operation is with reference to embodiment 1, and the result is referring to table 8,
Table 8. cross validation 4-1
Sample L48784 X83300 ?A1620827 AI494291 ?NM_020976 ?BF433657 J02959 ?BC022313 ?NM_000904 ?NM_004994
The training group T2 11386.6 ?658.7 730.5 553.5 963.8 2495.1 10854.5 4182.7 1516.9 4475.7
Whether median test group difference 1T mean N difference in means 2 meets T3T4T5N1N2N3N5T1 5944.1 5007.4 6973.1 21113.1 10817.5 13886.1 17733.3 11102.05 6615.2-the 4486.85 7327.8 15887.5-8559.7th, 496.2 453.5 703.5 773 782.5 1022.6 1396.6 738.25 340.3-the 397.95 577.975 993.675-415.7th, 1018.2 890.8 1478.2 2161.5 1772.8 1846.8 1949.2 1625.5 657.9-967.6 1029.425 1932.575-903.15th, 698.7 484.8 673.4 919.2 1117.8 1752.5 1,110 808.95 708.1-the 100.85 602.6 1224.875-622.275th, 522.8 745.3 642.2 1460.3 1194.4 1253.1 1367.2 1079.1 508.6-570.5 718.525 1318.75-600.225th, 3439.2 3504.5 2453.1 1374.7 2041.6 1114.9 1311.9 2247.35 4103.5 1856.15 2972.975 1460.775 1512.2 are 6894.9 10304.2 8012.7 5454.1 3179.9 2481.6 2819.9 6174.5 7571.2 1396.7 9016.575 3483.875 5532.7 are 3176.2 5365.8 2728.2 1,524 1,808 1132.9 1757.6 2268.1 3893.3 1625.2 3863.225 1555.625 2307.6 are 5390.5 4523.9 2784.2 1,137 1649.9 1678.9 770.6 1664.4 4635.3 2970.9 3553.875 1309.1 2244.775 are 7375.7 4207.6 3860.3 2043.6 2,940 1351.5 2171.3 3400.15 10756.1 7355.95 4979.825 2126.6 2853.225 are
In the table 8: coincidence rate is 10/10.Infer that tested sample is a carcinoma of the pancreas.
Embodiment 9
Operation is with reference to embodiment 1, and the result is referring to table 9,
Table 9. cross validation 4-2
Sample L48784 X83300 AI620827 AI494291 NM_020970 ?BF433657 ?J02959 BC022313 ?NM_000904 NM_004994
Whether training group median test group difference 1T mean N difference in means 2 meets ?T2?T3?T4?T5?N1?N2?N3?N5?N4 11386.6 5944.1 5007.4 6973.1 21113.1 10817.5 13886.1 17733.3 11102.05 16847.6 5745.55 7327.8 15887.5-8559.7 are not 658.7 496.2 453.5 703.5 773 782.5 1022.6 1396.6 738.25 1836.3 1098.05 577.975 993.675-415.7 are not 730.5 1018.2 890.8 1478.2 2161.5 1772.8 1846.8 1949.2 1625.5 2274.3 648.8 1029.425 1932.575-903.15 are not 553.5 698.7 484.8 673.4 919.2 1117.8 1752.5 1,110 808.95 1,455 646.05 602.6 1224.875-622.275 are not 963.8 522.8 745.3 642.2 1460.3 1194.4 1253.1 1367.2 1079.1 1472.4 393.3 718.525 1318.75-600.225 are not 2495.1 3439.2 3504.5 2453.1 1374.7 2041.6 1114.9 1311.9 2247.35 1231.2-1016.15 2972.975 1460.775 1512.2 are not 10854.5 6894.9 10304.2 8012.7 5454.1 3179.9 2481.6 2819.9 6174.5 3431.4-2743.1 9016.575 3483.875 5532.7 are not 4182.7 3176.2 5365.8 2728.2 1,524 1,808 1132.9 1757.6 2268.1 1102-1166.1 3863.225 1555.625 2307.6 are not 1516.9 5390.5 4523.9 2784.2 1,137 1649.9 1678.9 770.6 1664.4 1497.5-166.9 3553.875 1309.1 2244.775 are not 4475.7 7375.7 4207.6 3860.3 2043.6 2,940 1351.5 2171.3 3400.15 1300.5-2099.65 4979.825 2126.6 2853.225 are not
In the table 9: the rate of not meeting is 10/10.Infer the PBMC of tested sample for the normal people.
Embodiment 10
Operation is with reference to embodiment 1, and the result is referring to table 10,
Table 10. cross validation 5-1
Sample L48784 X83300 AI620827 ?AI494291 NM_020970 BF433657 J02959 ?BC022313 NM_000904 NM_004994
Whether training group median test group difference 1T mean N difference in means 2 meets ?T2?T3?T4?T5?N1?N2?N3?N4?T1 11386.6 5944.1 5007.4 6973.1 21113.1 10817.5 13886.1 16847.6 11102.05 6615.2-the 4486.85 7327.8 15666.075-8338.275th, 658.7 496.2 453.5 703.5 773 782.5 1022.6 1836.3 738.25 340.3-the 397.95 577.975 1103.6-525.625th, 730.5 1018.2 890.8 1478.2 2161.5 1772.8 1,846 8 2274.3 1625.5 657.9-the 967.6 1029.425 2013.85-984.425th, 553.5 698.7 484.8 673.4 919.2 1117.8 1752.5 1,455 808.95 708.1-the 100.85 602.6 1311.125-708.525th, 963.8 522.8 745.3 642.2 1460.3 1194.4 1253.1 1472.4 1079.1 508.6-570.5 718.525 1345.05-626.525th, 2495.1 3439.2 3504.5 2453.1 1374.7 2041.6 1114.9 1231.2 2247.35 4103.5 1856.15 2972.975 1440.6 1532.375 are 10854.5 6894.9 10304.2 8012.7 5454.1 3179.9 2481.6 3431.4 6174.5 7571.2 1396.7 9016.575 3636.75 5379.825 are 4182.7 3176.2 5365.8 2728.2 1,524 1,808 1132.9 1,102 2268.1 3893.3 1625.2 3863.225 1391.725 2471.5 are 1516.9 5390.5 4523.9 2784.2 1,137 1649.9 1678.9 1497.5 1664.4 4635.3 2970.9 3553.875 1490.825 2063.05 are 4475.7 7375.7 4207.6 3860.3 2043.6 2,940 1351.5 1300.5 3400.15 10756.1 7355.95 4979.825 1908.9 3070.925 are
In the table 10: coincidence rate is 10/10.Infer that tested sample is carcinoma of the pancreas patient's PBMC.
Embodiment 11
Operation is with reference to embodiment 1, and the result is referring to table 11,
Table 11 cross validation 5-2
Sample L48784 X83300 AI620827 AI494291 NM_020970 BF433657 J02959 BC022313 NM_000904 ?NM_004994
Training group median test group difference 1T mean N mean ?T2?T3?T4?T5?N1?N2?N3?N4?N5 ?11386.6?5944.1?5007.4?6973.1?21113.1?10817.5?13886.1?16847.6?11102.05?17733.3?6631.25?7327.8?15666.075 658.7 496.2 453.5 703.5 773 782.5 1022.6 1836.3 738.25 1396.6 658.35 577.975 1103.6 730.5 1018.2 890.8 1478.2 2161.5 1772.8 1846.8 2274.3 1625.5 1949.2 323.7 1029.425 2013.85 553.5 698.7 484.8 673.4 919?2 1117.8 1752.5 1455 808.95 1110 301.05 602.6 1311.125 963.8 522.8 745.3 642.2 1460.3 1194.4 1253.1 1472.4 1079.1 1367.2 288.1 718.525 1345.05 2495.1 3439.2 3504.5 2453.1 1374.7 2041.6 1114.9 1231.2 2247.35 1311.9 -935.45 2972.975 1440.6 ?10854.5?6894.9?10304.2?8012.7?5454.1?3179.9?2481.6?3431.4?6174.5?2819.9?-3354.6?9016.575?3636.75 4182.7 3176.2 5365.8 2728.2 1524 1808 1132.9 1102 2268.1 1757.6 -510.5 3863.225 1391.725 1516.9 5390.5 4523.9 2784.2 1137 1649.9 1678.9 1497.5 1664.4 770.6 -893.8 3553.875 1490.825 4475.7 7375.7 4207.6 3860.3 2043.6 2940 1351.5 1300.5 3400.15 2171.3 -1228.85 4979.825 1908.9
Whether differ from 2 meets -8338.275 are not -525.625 are not -984.425 are not -708.525 are not -626.525 are not 1532.375 not 5379.825 not 2471.5 not 2063.05 not 3070.925 not
In the table 11: the rate of not meeting is 10/10.Infer the PBMC of tested sample for the normal people.
Embodiment 12
Operation is with reference to embodiment 1, and the result is referring to table 12,
Table 12 cross validation 6-1
Sample L48784 X83300 AI620827 AI494291 NM_020970 BF433657 ?J02959 BC022313 NM_000904 NM_004994
Whether training group median test group difference 1T mean N difference in means 2 meets Whether T1 T3 T4 T5 N2 N3 N4 N5 meets 6615.2 5944.1 5007.4 6973.1 10817.5 13886.1 16847.6 17733.3 8895.3 11386.6 2491.3 6134.95 14821.125-8686.175 are not 340.3 496.2 453.5 703.5 782.5 1022.6 1836.3 1396.6 743 658.7-84.3 498.375 1259.5-761.125th, 657.9 1018.2 890.8 1478.2 1772.8 1846.8 2,274 3 1949.2 1625.5 730.5-the 895 1011.275 1960.775-949.5th, 708.1 698.7 484.8 673.4 1117.8 1752.5 1,455 1,110 909.05 553.5-the 355.55 641.25 1358.825-717.575th, 508.6 522.8 745.3 642.2 1194.4 1253.1 1472.4 1367.2 969.85 963.8-6.05 604.725 1321.775-717.05th, 4103.5 3439.2 3504.5 2453.1 2041.6 1114.9 1231.2 1311.9 2247.35 2495.1 247.75 3375.075 1424.9 1950.175 are 7571.2 6894.9 10304.2 8012.7 3179.9 2481.6 3431.4 2819.9 5163.15 10854.5 5691.35 8195.75 2978.2 5217.55 are 3893.3 3176.2 5365.8 2728.2 1,808 1132.9 1,102 1757.6 2268.1 4182.7 1914.6 3790.875 1450.125 2340.75 are 4635.3 5390.5 4523.9 2784.2 1649.9 1678.9 1497.5 770.6 2231.55 1516.9-714.65 4333.475 1399.225 2934.25 are not 10756.1 7375.7 4207.6 3860.3 2,940 1351.5 1300.5 2171.3 3400.15 4475.7 1075.55 6549.925 1940.825 4609.1 are
In the table 12: coincidence rate is 8/10.Infer that tested sample is a carcinoma of the pancreas.
Embodiment 13
Operation is with reference to embodiment 1, and the result is referring to table 13,
Table 13. cross validation 6-2
Sample L48784 ?X83300 AI620827 AI494291 NM_020970 BF433657 ?J02959 ?BC022313 ?NM_000904 NM_004994
The training group ?T1?T3?T4?T5?N2?N3?N4?N5 ?6615.2?5944.1?5007.4?6973.1?10817.5?13886.1?16847.6?17733.3 340.3 496.2 453.5 703.5 782.5 1022.6 1836.3 1396.6 657.9 1018.2 890.8 1478.2 1772.8 1846.8 2274.3 1949.2 708.1 698.7 484.8 673.4 1117.8 1752.5 1455 1110 508.6 522.8 745.3 642.2 1194.4 1253.1 1472.4 1367.2 ?4103.5?3439.2?3504.5?2453.1?2041.6?1114.9?1231.2?1311.9 ?7571.2?6894.9?10304.2?8012.7?3179.9?2481.6?3431.4?2819.9 3893.3 3176.2 5365.8 2728.2 1808 1132.9 1102 1757.6 4635.3 5390.5 4523.9 2784.2 1649.9 1678.9 1497.5 770.6 10756.1 7375.7 4207.6 3860.3 2940 1351.5 1300.5 2171.3
Whether median test group difference 1T mean N difference in means 2 meets N1 8895.3 21113.1 12217.8 6134.95 14821.125-8686.175 are not 743 773 30 498.375 1259.5-761.125 are not 1625.5 2161.5 536 1011.275 1960.775-949.5 are not 909.05 919.2 10.15 641.25 1358.825-717.575 are not 969.85 1460.3 490.45 604.725 1321.775-717.05 are not 2247.35 1374.7-872.65 3375.075 1424.9 1950.175 are not 5163.15 5454.1 290.95 8195.75 2978.2 5217.55 are 2268.1 1524-744.1 3790.875 1450.125 2340.75 are not 2231.55 1137-1094.55 4333.475 1399.225 2934.25 are not 3400.15 2043.6-1356.55 6549.925 1940.825 4609.1 are not
In the table 13: the rate of not meeting is 9/10.Infer the PBMC of tested sample for the normal people.
Embodiment 14
Operation is with reference to embodiment 1, and the result is referring to table 14,
Table 14. cross validation 7-1
Sample L48784 X83300 AI620827 AI494291 NM020970 BF433657 J02959 BC022313 ?NM_000904 NM_004994
Whether training group median test group differs from 1 T mean N difference in means 2 and meets ?T1?T3?T4?T5?N1?N3?N4?N5?T2 6615.2 5944.1 5007.4 6973.1 21113.1 13886.1 16847.6 17733.3 10429.6 11386.6 957 6134.95 17395.025-11260.075 are not 340.3 496.2 453.5 703.5 773 1022.6 1836.3 1396.6 738.25 658.7-79.55 498.375 1257.125-758.75th, 657.9 1018.2 890.8 1478.2 2161.5 1846.8 2274.3 1949.2 1662.5 730.5-the 932 1011.275 2057.95-1046.675th, 708.1 698.7 484.8 673.4 919.2 1752.5 1,455 1,110 813.65 553.5-the 260.15 641.25 1309.175-667.925th, 508.6 522.8 745.3 642.2 1460.3 1253.1 1472.4 1367.2 999.2 963.8-35.4 604.725 1388.25-783.525th, 4103.5 3439.2 3504.5 2453.1 1374.7 1114.9 1231.2 1311.9 1913.9 2495.1 581.2 3375.075 1258.175 2116.9 are 7571.26894.910304.28012.75454.12481.63431.42819.96174.51 0854.546808195.753546.754649 is 3893.3 3176.2 5365.8 2728.2 1,524 1132.9 1,102 1757.6 2242.9 4182.7 1939.8 3790.875 1379.125 2411.75 are 4635.3 5390.5 4523.9 2784.2 1,137 1678.9 1497.5 770.6 2231.55 1516.9-714.65 4333.475 1,271 3062.475 are not 10756.1 7375.7 4,207 6 3860.3 2043.6 1351.5 1300.5 2171.3 3015.8 4475.7 1459.9 6549.925 1716.725 4833.2 are
In the table 14: coincidence rate is 8/10.Infer that tested sample is carcinoma of the pancreas patient's PBMC.
Embodiment 15
Operation is with reference to embodiment 1, and the result is referring to table 15,
Table 15. cross validation 7-2
Sample L48784 ?X83300 ?AI620827 ?AI494291 ?NM?020970 BF433657 ?j02959 BC022313 ?NM_000904 ?NM_004994
The training group ?T1?T3?T4 ?6615.2?5944.1?5007.4 340.3 496.2 453.5 657.9 1018.2 890.8 708.1 698.7 484.8 508.6 522.8 745.3 4103.5 3439.2 3504.5 7571.2 6894.9 10304.2 3893.3 3176.2 5365.8 4635.3 5390.5 4523.9 10756.1 7375.7 4207.6
Whether median test group difference 1T mean N difference in means 2 meets T5N1N3N4N5N2 6973.1 21113.1 13886.1 16847.6 17733.3 10429.6 10817.5 387.9 6134.95 17395.025-11260.075 are not 703.5 773 1022.6 1836.3 1396.6 738.25 782.5 44.25 498.375 1257.125-758.75 are not 1478.2 2161.5 1846.8 2274.3 1949.2 1662.5 1772.8 110.3 1011.275 2057.95-1046.675 are not 673.4 919.2 1752.5 1,455 1,110 813.65 1117.8 304.15 641.25 1309.175-667.925 are not 642.2 1460.3 1253.1 1472.4 1367.2 999.2 1194.4 195.2 604.725 1388.25-783.525 are not 2453.1 1374.7 1114.9 1231.2 1311.9 1913.9 2041.6 127.7 3375.075 1258.175 2116.9 are 8012.75454.12481.63431.42819.96174.53179.9-2994.68195.75 3546.754649 are not 2728.2 1,524 1132.9 1,102 1757.6 2242.9 1808-434.9 37903.875 1379.125 2411.75 are not 2784.2 1,137 1678.9 1497.5 770.6 2231.55 1649.9-581.65 4333.475 1,271 3062.475 are not 3860.3 2043.6 1351.5 1300.5 2171.3 3015.8 2940-75.8 6549.925 1716.725 4833.2 are not
In the table 15: the rate of not meeting is 9/10.Infer the PBMC of tested sample for the normal people.
Embodiment 16
Operation is with reference to embodiment 1, and the result is referring to table 16,
Table 16 cross validation 8-1
Sample L48784 X83300 AI620827 AI494291 NM?020970 BF433657 J02959 ?BC022313 NM_000904 ?NM_004994
Whether training group median test group difference 1T mean N difference in means 2 meets ?T1?T3?T4?T5?N1?N2?N4?N5?T2 6615.2 5944.1 5007.4 6973.1 21113.1 10817.5 16847.6 17733.3 8895.3 11386.6 2491.3 6134.95 16627.875-10492.925 are not 340.3 496.2 453.5 703.5 773 782.5 1836.3 1396.6 738.25 658.7-79.55 498.375 1197.1-698.725th, 657.9 1018.2 890.8 1478.2 2161.5 1772.8 2274.3 1949.2 1625.5 730.5-the 895 1011.275 2039.45-1028.175th, 708.1 698.7 484.8 673.4 919.2 1117.8 1,455 1,110 813.65 553.5-the 260.15 641.25 1150.5-509.25th, 508.6 522.8 745.3 642.2 1460.3 1194.4 1472.4 1367.2 969.85 963.8-6.05 604.725 1373.575-768.85th, 4103.5 3439.2 3504.5 2453.1 1374.7 2041.6 1231.2 1311.9 2247.35 2495.1 247.75 3375.075 1489.85 1885.225 are 7571.2 6894.9 10304.2 8012.7 5454.1 3179.9 3431.4 2819.9 6174.5 10854.5 4,680 8195.75 3721.325 4474.425 are 3893.3 3,176 2 5365.8 2728.2 1,524 1,808 1,102 1757.6 2268.1 4182.7 1914.6 3790.875 1547.9 2242.975 are 4635.3 5390.5 4523.9 2784.2 1,137 1649.9 1497.5 770.6 2217.05 1516.9-700.15 4333.475 1263.75 3069.725 are not 10756.1 7375.7 4207.6 3860.3 2043.6 2,940 1300.5 2171.3 3400.15 4475.7 1075.55 6549.925 2113.85 4436.075 are
In the table 16: coincidence rate is 8/10.Infer that tested sample is carcinoma of the pancreas patient's PBMC.
Embodiment 17
Operation is with reference to embodiment 1, and the result is referring to table 17,
Table 17. cross validation 8-2
Sample ?L48784 X83300 AI620827 AI494291 NM?020970 BF433657 J02959 BC0223?13 ?NM_000904 NM_004994
Whether training group median test group difference 1T mean N difference in means 2 meets T1T3T4T5N1N2N4N5N3 6615.2 5944.1 5007.4 6973.1 21113.1 10817.5 16847.6 17733.3 8895.3 13886.1 4990.8 6134.95 16627.875-10492.925 are not 340.3 496.2 453.5 703.5 773 782.5 1836.3 1396.6 738.25 1022.6 284.35 498.375 1197.1-698.725 are not 657.9 1018.2 890.8 1478.2 2161.5 1772.8 2274.3 1949.2 1625.5 1846.8 221.3 1011.275 2039.45-1028.175 are not 708.1 698.7 484.8 673.4 919.2 1117.8 1,455 1,110 813.65 1752.5 938.85 641.25 1150.5-509.25 are not 508.6 522.8 745.3 642.2 1460.3 1194.4 1472.4 1367.2 969.85 1253.1 283.25 604.725 1373.575-768.85 are not 4103.5 3439.2 3504.5 2453.1 1374.7 2041.6 1231.2 1311.9 2247.35 1114.9-1132.45 3375.075 1489.85 1885.225 are not 7571.2 6894.9 10304.2 8012.7 5454.1 3179.9 3431.4 2819.9 6174.5 2481.6-3692.9 8195.75 3721.325 4474.425 are not 3893.3 3176.2 5365.8 2728.2 1,524 1,808 1,102 1757.6 2268.1 1132.9-1135.2 3790.875 1547.9 2242.975 are not 4635.3 5390.5 4523.9 2784.2 1,137 1649.9 1497.5 770.6 2217.05 1678.9-538.15 4333.475 1263.75 3069.725 are not 10756.1 7375.7 4207.6 3860.3 2043.6 2,940 1300.5 2171.3 3400.15 1351.5-2048.65 6549.925 2113.85 4436.075 are not
In the table 17: the rate of not meeting is 10/10.Infer the PBMC of tested sample for the normal people.
Embodiment 18
Operation is with reference to embodiment 1, and the result is referring to table 18,
Table 18. cross validation 9-1
Sample L48784 ?X83300 ?A1620827 A1494291 ?NM?020970 BF433657 J02959 BC0223?13 NM_000904 NM_004994
Whether training group median test group differs from 1 T mean N difference in means 2 and meets ?T?T3?T4?T5?N1?N2?N3?N5?T2 6615.2 5944.1 5007.4 6973.1 21113.1 10817.5 13886.1 17733.3 8895.3 11386.6 2491.3 6134.95 15887.5-9752.55 are not 340.3 496.2 453.5 703.5 773 782.5 1022.6 1396.6 738.25 658.7-79.55 498.375 993.675-495.3rd, 657.9 1018.2 890.8 1478.2 2161.5 1772.8 1846.8 1949.2 1625.5 730.5-the 895 1011.275 1932.575-921.3rd, 708.1 698.7 484.8 673.4 919.2 1117.8 1752.5 1,110 813.65 553.5-the 260.15 641.25 1224.875-583.625th, 508.6 522.8 745.3 642.2 1460.3 1194.4 1253.1 1367.2 969.85 963.8-6.05 604.725 1318.75-714.025th, 4103.5 3439.2 3504.5 2453.1 1374.7 2041.6 1114.9 1311.9 2247.35 2495.1 247.75 3375.075 1460.775 1914.3 are 7571.2 6894.9 10304.2 8012.7 5454.1 3179.9 2481.6 2819.9 6174.5 10854.5 4,680 8195.75 3483.875 4711.875 are 3893.3 3176.2 5365.8 2728.2 1,524 1,808 1132.9 1757.6 2268.1 4182.7 1914.6 3790.875 1555.625 2235.25 are 4635.3 5390.5 4523.9 2784.2 1,137 1649.9 1678.9 770.6 2231.55 1516.9-714.65 4333.475 1309.1 3024.375 are not 10756.1 7375.7 4207.6 3,860 3 2043.6 2,940 1351.5 2171.3 3400.15 4475.7 1075.55 6549.925 2126.6 4423.325 are
In the table 18: coincidence rate is 8/10.Infer that tested sample is carcinoma of the pancreas patient's PBMC.
Embodiment 19
Operation is with reference to embodiment 1, and the result is referring to table 19,
Table 19. cross validation 9-2
Sample L48784 X83300 A1620827 A1494291 NM?020970 BF433657 J02959 BC022313 NM_000904 NM_004994
Whether training group median test group difference 1T mean N difference in means 2 meets ?T1?T3?T4?T5?N1?N2?N3?N5?N4 6615.25944.15007.46973.121113.110817.513886.117733.38895 .316847.67952.36134.9515887.5-9752.55 is not 340.3 496.2 453.5 703.5 773 782.5 1022.6 1396.6 738.25 1836.3 1098.05 498.375 993.675-495.3 are not 657.9 1018.2 890.8 1478.2 2161.5 1772.8 1846.8 1949.2 1625.5 2274.3 648.8 1011.275 1932.575-921.3 are not 708.1 698.7 484.8 673.4 919.2 1117.8 1752.5 1,110 813.65 1,465 641.35 641.25 1224.875-583.625 are not 508.6 522.8 745.3 642.2 1460.3 1194.4 1253.1 1367.2 969.85 1472.4 502.55 604.725 1318.75-714.025 are not 4103.5 3439.2 3504.5 2453.1 1374.7 2041.6 1114.9 1311.9 2247.35 1231.2 1016.15 3375.075 1460.775 1914.3 are not 7571.2 6894.9 10304.2 8012.7 5454.1 3179.9 2481.6 2819.9 6174.5 3431.4-2743.1 8195.75 3483.875 4711.875 are not 3893.3 3176.2 5365.8 2728.2 1,524 1,808 1132.9 1757.6 2268.1 1102-1166.1 3790.875 1555.625 2235.25 are not 4635.3 5390.5 4523.9 2784.2 1,137 1649.9 1678.9 770.6 2231.55 1497.5 734.05 4333.475 1309.1 3024.375 are not 10756.1 7375.7 4207.6 3860.3 2043.6 2,940 1351.5 2171.3 3400.15 1300.5-2099.65 6549.925 2126.6 4423.325 are not
In the table 19: the rate of not meeting is 10/10.Infer the PBMC of tested sample for the normal people.
Embodiment 20
Operation is with reference to embodiment 1, and the result is referring to table 20,
Table 20. cross validation 10-1
Sample L48784 X83300 AI620827 AI494291 ?NM?020970 BF433657 J02959 BC022313 NM_000904 NM_004994
Training group median test group differs from 1 T1 T3 T4 T5 N1 N2 N3 N4 T2 6615.2 5944.1 5007.4 6973.1 21113.1 10817.5 13886.1 16847.6 8895.3 11386.6 2491.3 340.3 496.2 453.5 703.5 773 782.5 1022.6 1836.3 738.25 658.7 79.55 657.9 1018.2 890.8 1478.2 2161.5 1772.8 1846.8 2274.3 1625.5 730.5 -895 708.1 698.7 484.8 673.4 919.2 1117.8 1752.5 1455 813.65 553.5 -260.15 ?508.6 ?522.8 ?745.3 ?642.2 ?1460.3 ?1194.4 ?1253.1 ?1472.4 ?969.85 ?963.8 ?-6.05 4103.5 3439.2 3504.5 2453.1 1374.7 2041.6 1114.9 1231.2 2247.35 2495.1 247.75 7571.2 6894.9 10304.2 8012.7 5454.1 3179.9 2481.6 3431.4 6174.5 10854.5 4680 3893.3 3176.2 5365.8 2728.2 1524 1808 1132.9 1102 2268.1 4182.7 1914.6 4635.3 5390.5 4523.9 2784.2 1137 1649.9 1678.9 1497.5 2231.55 1516.9 -714.65 10756.1 7375.7 4207.6 3860.3 2043.6 2940 1351.5 1300.5 3400.15 4475.7 1075.55
Whether T mean N difference in means 2 meets 6134.9515666.075-9531.125 not 498.375 1103.6-605.225th, 1011.275 2013.85 1002.575 are 641.2 the 5 1311.125-669.875th, 604.7251345.05-740.325 be 3375.075 1440.6 1934.475 are 8195.753636.754559 be 3790.8751391.7252399.15 be 4333.4751490.8252842.65 not 6549.9251908.94641.025 be
In the table 20: coincidence rate is 8/10.Infer that tested sample is carcinoma of the pancreas patient's PBMC.
Embodiment 21
Operation is with reference to embodiment 1, and the result is referring to table 21,
Table 21. cross validation 10-2
Sample ?L48784 X83300 ?AI620827 AI494291 NM?020970 BF433657 ?J02959 BC022313 NM_000904 NM_004994
Whether training group median test group differs from 1 T mean N difference in means 2 and meets ?T1?T3?T4?T5?N1?N2?N3?N4?N5 6615.2 5944.1 5007.4 6973.1 21113.1 10817.5 13886.1 16847.6 8895.3 17733.3 8,838 6134.95 15666.075-9531.125 are not 340.3 496.2 453.5 703.5 773 782.5 1022.6 1836.3 738.25 1396.6 658.35 498.375 1103.6-605.225 are not 657.9 1018.2 890.8 1478.2 2161.5 1772.8 1846.8 2274.3 1625.5 1949.2 323.7 1011.275 2013.85-1002.575 are not 708.1 698.7 484.8 673.4 919.2 1117.8 1752.5 1,456 813.65 1,110 296.35 641.25 1311.125-669.875 are not 508.6 522.8 745.3 642.2 1460.3 1194.4 1253.1 1472.4 969.85 1367.2 397.35 604.725 1345.05-740.325 are not 4103.5 3439.2 3504.5 2453.1 1374.7 2041.6 1114.9 1231.2 2247.35 1311.9 935.45 3375.075 1440.6 1934.475 are not 7571.2 6894.9 10304.2 8012.7 5454.1 3179.9 2481.6 3431.4 6174.5 2819.9-3354.6 8195.75 3636.75 4559 are not 3893.3 3176.2 5365.8 2728.2 1,524 1,808 1132.9 1,102 2268.1 1757.6-510.5 3790.875 1391.725 2399.15 are not 4635.3 5390.5 4523.9 2784.2 1,137 1649.9 1678.9 1497.5 2231.55 770.6-1460.95 4333.475 1490.825 2842.65 are not 10756.1 7375.7 4207.6 3860.3 2043.6 2,940 1351.5 1300.5 3400.15 2171.3-1228.85 6549.925 1908.9 4641.025 are not
In the table 21: the rate of not meeting is 10/10.Infer the PBMC of tested sample for the normal people.
Embodiment 22
Operation is with reference to embodiment 1, and the result is referring to table 22.
Table 22. cross validation 11-1
Sample L48784 X83300 AI620827 AI494291 NM?020970 BF433657 J02959 BC022313 ?NM_000904 ?NM_004994
The training group T1T2 ?6615.2?11386.6 ?340.3?658.7 657.9 730.5 708.1 553.5 508.6 963.8 4103.5 2495.1 7571.2 10854.5 3893.3 4182.7 4635.3 1516.9 10756.1 4475.7
Whether median test group difference 1T mean N difference in means 2 meets ?T4?T5?N2?N3?N4?N5?T3 5,007 4 6973.1 10817.5 13886.1 16847.6 17733.3 11102.05 5944.1-the 5157.95 7495.575 14821.125-7325.55th, 453.5 703.5 782.5 1022.6 1836.3 1396.6 743 496.2-246.8 539 1259.5-720.5th, 890.8 1478.2 1772.8 1846.8 2274.3 1949.2 1625.5 1018.2-607.3 939.35 1960.775-1021.425th, 484.8 673.4 1117.8 1752.5 1,455 1,110 909.05 698.7-the 210.35 604.95 1358.825-753.875th, 745.3 642.2 1194.4 1253.1 1472.4 1367.2 1079.1 522.8-556.3 714.975 1321.775-606.8th, 3504.5 2453.1 2041.6 1114.9 1231.2 1311.9 2247.35 3439.2 1191.85 3139.05 1424.9 1714.15 are 10304.2 8012.7 3179.9 2481.6 3431.4 2819.9 5501.3 6894.9 1393.6 9185.65 2978.2 6207.45 are 5365.8 2728.2 1,808 1132.9 1,102 1757.6 2268.1 3176.2 908.1 4042.5 1450.125 2592.375 are 4523.9 2784.2 1649.9 1678.9 1497.5 770.6 1664.4 5390.5 3726.1 3365.075 1399.225 1965.85 are 4207.6 3860.3 2,940 1351.5 1300.5 2171.3 3400.15 7375.7 3975.55 5824.925 1940.825 3884.1 are
In the table 22: coincidence rate is 10/10.Infer that tested sample is carcinoma of the pancreas patient's PBMC.
Embodiment 23
Operation is with reference to embodiment 1, and the result is referring to table 23.
Table 23. cross validation 11-2
Sample L48784 ?X83300 AI620827 AI494291 ?NM?020970 ?3F433657 ?J02959 BC0223?13 NM_000904 ?NM_004994
Whether training group median test group differs from 1 T mean N difference in means 2 and meets ?T1?T2?T4?T5?N2?N3?N4?N5?N1 6615.2 11386.6 5007.4 6973.1 10817.5 13886.1 16847.6 17733.3 11102.05 21113.1 10011.05 7495.575 14821.125-7325.55 are not 340.3 658.7 453.5 703.5 782.5 1022.6 1836.3 1396.6 743 773 30 539 1259.5-720.5 are not 657.9 730.5 890.8 1478.2 1772.8 1846.8 2274.3 1949.2 1625.5 2161.5 536 939.35 1960.775-1021.425 are not 708.1 553.5 484.8 673.4 1117.8 1752.5 1,455 1,110 909.05 919.2 10.15 604.95 1358.825 753.875 are not 508.6 963.8 745.3 642.2 1194.4 1253.1 1472.4 1367.2 1079.1 1460.3 381.2 714.975 1321.775-606.8 are not 4103.5 2495.1 3504.5 2453.1 2041.6 1114.9 1231.2 1311.9 2247.35 1374.7 872.65 3139.05 1424.9 1714.15 are not 7571.2 10854.5 10304.2 8012.7 3179.9 2481.6 3431.4 2819.9 5501.3 5454.1-47.2 9185.65 2978.2 6207.45 are not 3893.3 4182.7 5365.8 2728.2 1,808 1132.9 1,102 1757.6 2268.1 1524-744.1 4042.5 1450.125 2592.375 are not 4635.3 1516.9 4523.9 2784.2 1649.9 1678.9 1497.5 770.6 1664.4 1,137 527.4 3365.075 1399.225 1965.85 are not 10756.1 4475.7 4207.6 3860.3 2,940 1351.5 1300.5 2171.3 3400.15 2043.6 1356.55 5824.925 1940.825 3884.1 are not
In the table 23: the rate of not meeting is 10/10.Infer the PBMC of tested sample for the normal people.
Embodiment 24
Operation is with reference to embodiment 1, and the result is referring to table 24.
Table 24 cross validation 12-1
Sample L48784 ?X83300 ?AI620827 AI494291 ?NM?020970 BF433657 ?J02959 BC0223?13 NM_000904 NM_004994
Whether training group median test group difference 1T mean N difference in means 2 meets ?T1?T2?T4?T5?N1?N3?N4?N5?T3 6615.2 11386.6 5007.4 6973.1 21113.1 13886.1 16847.6 17733.3 12636.35 5944.1-the 6692.25 7495.575 17395.025-9899.45th, 340.3 658.7 453.5 703.5 773 1022.6 1836.3 1396.6 738.25 496.2-the 242.05 539 1257.125-718.125th, 657.9 730.5 890.8 1478.2 2161.5 1846.8 2274.3 1949.2 1662.5 1018.2-644.3 939.35 2057.95-1118.6th, 708.1 553.5 484.8 673.4 919.2 1752.5 1,455 1,110 813.65 698.7-the 114.95 604.95 1309.175-704.225th, 508.6 963.8 745.3 642.2 1460.3 1253.1 1472.4 1367.2 1108.45 522.8-the 585.65 714.975 1388.25-673.275th, 4103.5 2495.1 3504.5 2453.1 1374.7 1114.9 1231.2 1311.9 1913.9 3439.2 1525.3 3139.05 1258.175 1880.875 are 7571.2 10854.5 10304.2 8012.7 5454.1 2481.6 3431.4 2819.9 6512.65 6894.9 382.25 9185.65 3546.75 5638.9 are 3893.3 4182.7 5365.8 2728.2 1,524 1132.9 1,102 1757.6 2242.9 3176.2 933.3 4042.5 1379.125 2663.375 are 4635.3 1516.9 4523.9 2784.2 1,137 1678.9 1497.5 770.6 1597.9 5390.5 3792.6 3365.075 1,271 2094.075 are 10756.1 4475.7 4207.6 3860.3 2043.6 1351.5 1300.5 2171.3 3015.8 7375.7 4359.9 5824.925 1716.725 4108.2 are
In the table 24: coincidence rate is 10/10.Infer that tested sample is carcinoma of the pancreas patient's PBMC.
Embodiment 25
Operation is with reference to embodiment 1, and the result is referring to table 25.
Table 25. cross validation 12-2
Sample L48784 ?X83300 A1620827 A1494291 NM?020970 ?BF433657 ?J02959 ?BC0223?13 ?NM_000904 NM_004994
Training group median ?T1?T2?T4?T5?N1?N3?N4?N5 ?6615.2?11386.6?5007.4?6973.1?21113.1?13886.1?16847.6?17733.3?12636.35 340.3 658.7 453.5 703.5 773 1022.6 1836.3 1396.6 738.25 657.9 730.5 890.8 1478.2 2161.5 1846.8 2274.3 1949.2 1662.5 708.1 553.5 484.8 673.4 919.2 1752.5 1455 1110 813.65 508.6 963?8 745.3 642.2 1460.3 1253.1 1472.4 1367.2 1108.45 4103.5 2495.1 3504.5 2453.1 1374.7 1114.9 1231.2 1311.9 1913.9 ?7571.2?10854.5?10304.2?8012.7?5454.1?2481.6?3431.4?2819.9?6512.65 3893.3 4182.7 5365.8 2728.2 1524 1132.9 1102 1757.6 2242.9 4635.3 1516?9 4523.9 2784.2 1137 1678.9 1497.5 770.6 1597.9 10756.1 4475.7 4207.6 3860.3 2043.6 1351.5 1300.5 2171.3 3015.8
Whether test group difference 1T mean N difference in means 2 meets N2 10817.5 the 1818.85 7495.575 17395.025-9899.45th, 782.5 44.25 539 1257.125-718.125 are not 1772.8 110.3 939.35 2057.95-1118.6 are not 1117.8 304.15 604.95 1309.175-704.225 are not 1194.4 85.95 714.975 1388.25-673.275 are not 2041.6 127.7 3139.05 1258.175 1880.875 are 3179.9-3332.75 9185.65 3546.75 5638.9 are not 1808-434.9 4042.5 1379.125 2663.375 are not 1649.9 52 3365.075 1,271 2094.075 are 2940-75.8 5824.925 1716.725 4108.2 are not
In the table 25: the rate of not meeting is 7/10.Infer the PBMC of tested sample for the normal people.
Embodiment 26
Operation is with reference to embodiment 1, and the result is referring to table 26.
Table 26. cross validation 13-1
Sample L48784 X83300 ?AI620827 AI494291 ?NM_020970 ?BF433657 J02959 BC022313 ?NM_000904 ?NM_004994
Whether training group median test group difference 1T mean N difference in means 2 meets ?T1?T2?T4?T5?N1?N2?N4?N5?T3 6615.2 11386.6 5007.4 6973.1 21113.1 10817.5 16847.6 17733.3 11102.05 5944.1-the 5157.95 7495.575 16627.875-9132.3rd, 340.3 658.7 453.5 703.5 773 782.5 1836.3 1396.6 738.25 496.2-the 242.05 539 1197.1-658.1st, 657.9 730.5 890.8 1478.2 2161.5 1772.8 2274.3 1949.2 1625.5 1018.2-607.3 939.35 2039.45-1100.1st, 708.1 553.5 484.8 673.4 919.2 1117.8 1,455 1,110 813.65 698.7-the 114.95 604.95 1150.5-545.55th, 508.6 963.8 745.3 642.2 1460.3 1194.4 1472.4 1367.2 1079.1 522.8-556.3 714.975 1373.575-658.6th, 4103.5 2495.1 3504.5 2453.1 1374.7 2041.6 1231.2 1311.9 2247.35 3439.2 1191.85 3139.05 1489.85 1649.2 are 7571.2 10854.5 10304.2 8012.7 5454.1 3179.9 3431.4 2819.9 6512.65 6894.9 382.25 9185.65 3721.325 5464.325 are 3893.3 4182.7 5365.8 2728.2 1,524 1,808 1,102 1757.6 2268.1 3176.2 908.1 4042.5 1547.9 2494.6 are 4635.3 1516.9 4523.9 2784.2 1,137 1649.9 1497.5 770.6 1583.4 5390.5 3807.1 3365.075 1263.75 2101.325 are 10756.1 4,475 7 4207.6 3860.3 2043.6 2,940 1300.5 2171.3 3400.15 7375.7 3975.55 5824.925 2113.85 3711.075 are
In the table 26: coincidence rate is 10/10.Infer that tested sample is carcinoma of the pancreas patient's PBMC.
Embodiment 27
Operation is with reference to embodiment 1, and the result is referring to table 27.
Table 27. cross validation 13-2
Sample L48784 X83300 AI620827 AI494291 NM_020970 BF433657 J02959 BC0223 13 NM_000904 NM_004994
The training group T1 T2 T4 6615.2 11386.6 5007.4 340.3 658.7 453.5 657.9 730.5 890.8 708.1 553.5 484.8 508.6 963.8 745.3 4103.5 2495.1 3504.5 7571.2 10854.5 10304.2 3893.3 4182.7 5365.8 4635.3 1516.9 4523.9 10756.1 4475.7 4207.6
Whether median test group difference 1T mean N difference in means 2 meets ?T5?N1?N2?N4?N5?N3 6973.121113.110817.516847.617733.311102.0513886.12784.05 7495.5751.6627.875-9132.3 is not 703.5 773 782.5 1836.3 1396.6 738.25 1022.6 284.35 539 1197.1-658.1 are not 1478.2 2161.5 1772.8 2274.3 1949.2 1625.5 1846.8 221.3 939.35 2039.45 1100.1 are not 673.4 919.2 1117.8 1,455 1,110 813.65 1752.5 938.85 604.95 1150.5-545.55 are not 642.2 1460.3 1194.4 1472.4 1367.2 1079.1 1253.1 174 714.975 1373.575-658.6 are not 2453.1 1374.7 2041.6 1231.2 1311.9 2247.35 1114.9 1132.45 3139.05 1489.85 1649.2 are not 8012.7 5454.1 3179.9 3431.4 2819.9 6512.65 2481.6-4031.05 9185.65 3721.325 5464.325 are not 2728.2 1,524 1,808 1,102 1757.6 2268.1 1132.9 1135.2 4042.5 1547.9 2494.6 are not 2784.2 1,137 1649.9 1497.5 770.6 1583.4 1678.9 95.5 3365.075 1263.75 2101.325 are 3860.3 2043.6 2,940 1300.5 2171.3 3400.15 1351.5-2048.65 5824.925 2113.85 3711.075 are not
In the table 27: the rate of not meeting is 9/10.Infer the PBMC of tested sample for the normal people.
Embodiment 28
Operation is with reference to embodiment 1, and the result is referring to table 28.
Table 28 cross validation 14-1
Sample L48784 ?X83300 AI620827 AI494291 NM_020970 BF433657 ?J02959 ?BC022313 ?NM_000904 NM_004994
Whether training group median test group difference 1T mean N difference in means 2 meets ?T1?T2?T4?T5?N1?N2?N3?N5?T3 6615.2 11386.6 5007.4 6973.1 21113.1 10817.5 13886.1 17733.3 11102.05 5944.1-the 5157.95 7495.575 15887.5-8391.925th, 340.3 658.7 453.5 703.5 773 782.5 1022.6 1396.6 738.25 496.2 242.05 539 993.675 454.675 are 657.9 730.5 890.8 1478.2 2161.5 1772.8 1846.8 1949.2 1625.5 1018.2-607.3 939.35 1932.575-993.225th, 708.1 553.5 484.8 673.4 919.2 1117.8 1752.5 1,110 813.65 698.7-the 114.95 604.95 1224.875-619.925th, 508.6 963.8 745.3 642.2 1460.3 1194.4 1253.1 1367.2 1079.1 522.8-556.3 714.975 1318.75 603.775 are 4103.5 2495.1 3504.5 2453.1 1374.7 2041.6 1114.9 1311.9 2247.35 3439.2 1191.85 3139.05 1460.775 1678.275 are 7571.2 10854.5 10304.2 8012.7 5454.1 3179.9 2481.6 2819.9 6512.65 6894.9 382.25 9185.65 3483.875 5701.775 are 3893.3 4182.7 5365.8 2728.2 1,524 1,808 1132.9 1757.6 2268.1 3176.2 908.1 4042.5 1555.625 2486.875 are 4635.3 1516.9 4523.9 2784.2 1,137 1649.9 1678.9 770.6 1664.4 5390.5 3726.1 3365.075 1309.1 2055.975 are 10756.1 4475.7 4207.6 3860.3 2043.6 2,940 1351.5 2171.3 3400.15 7375.7 3975.55 5824.925 2126.6 3698.325 are
In the table 28: coincidence rate is 10/10.Infer that tested sample is carcinoma of the pancreas patient's PBMC.
Embodiment 29
Operation is with reference to embodiment 1, and the result is referring to table 29.
Table 29. cross validation 14-2
Sample L48784 X83300 AI620827 AI494291 NM_020970 BF433657 J02959 BC022313 NM_000904 NM_004994
Whether training group median test group difference 1T mean N difference in means 2 meets ?T1?T2?T4?T5?N1?N2?N3?N5?N4 6615.2 11386.6 5007.4 6973.1 21113.1 10817.5 13886.1 17733.3 11102.05 16847.6 5745.55 7495.575 15887.5-8391.925 are not 340.3 658.7 453.5 703.5 773 782.5 1022.6 1396.6 738.25 1836.3 1098.05 539 993.675-454.675 are not 657.9 730.5 890.8 1478.2 2161.5 1772.8 1846.8 1949.2 1625.5 2274.3 648.8 939.35 1932.575-993.225 are not 708.1 553.5 484.8 673.4 919.2 1117.8 1752.5 1,110 813.65 1,455 641.35 604.95 1224.875-619.925 are not 508.6 963.8 745.3 642.2 1460.3 1194.4 1253.1 1367.2 1079.1 1472.4 393.3 714.975 1318.75-603.775 are not 4103.5 2495.1 3504.5 2453.1 1374.7 2041.6 1114.9 1311.9 2247.35 1231.2-1016.15 3139.05 1460.775 1678.275 are not 7571.2 10854.5 10304.2 8012.7 5454.1 3179.9 2481.6 2819.9 6512.65 3431.4-3081.25 9185.65 3483.875 5701.775 are not 3893.3 4182.7 5365.8 2728.2 1,524 1,808 1132.9 1757.6 2268.1 1102-1166.1 4042.5 1555.625 2486.875 are not 4635.3 1516.9 4523.9 2784.2 1,137 1649.9 1678.9 770.6 1664.4 1497.5-166.9 3365.075 1309.1 2055.975 are not 10756.1 4475.7 4207.6 3860.3 2043.6 2,940 1351.5 2171.3 3400.15 1300.5-2099.65 5824.925 2126.6 3698.325 are not
In the table 29: the rate of not meeting is 10/10.Infer the PBMC of tested sample for the normal people.
Embodiment 30
Operation is with reference to embodiment 1, and the result is referring to table 30.
Table 30 cross validation 15-1
Sample L48784 ?X83300 ?AI620827 AI494291 NM_020970 ?BF433657 ?J02959 ?BC0223?13 NM_000904 NM_004994
Training group median test group difference 1T mean ?T1?T2?T4?T5?N1?N2?N3?N4?T3 6615.2 11386.6 5007.4 6973.1 21113.1 10817.5 13886.1 16847.6 11102.05 5944.1 -5157.95 7495.575 ?340.3 ?658.7 ?453.5 ?703.5 ?773 ?782.5 ?1022.6 ?1836.3 ?738.25 ?496.2 ?-242.05 ?539 ?657.9 ?730.5 ?890.8 ?1478.2 ?2161.5 ?1772.8 ?1846.8 ?2274.3 ?1625.5 ?1018.2 ?-607.3 ?939.35 708.1 553.5 484.8 673.4 919.2 1117.8 1752.5 1455 813.65 698.7 -114.95 604.95 508.6 963.8 745.3 642.2 1460.3 1194.4 1253.1 1472.4 1079.1 522.8 556.3 714.975 4103.5 2495.1 3504.5 2453.1 1374.7 2041.6 1114.9 1231.2 2247.35 3439.2 1191.85 3139.05 7571.2 10854.5 10304.2 8012.7 5454.1 3179.9 2481.6 3431.4 6512.65 6894.9 382.25 9185.65 3893.3 4182.7 5365.8 2728.2 1524 1808 1132.9 1102 2268.1 3176.2 908.1 4042.5 ?4635.3 ?1516.9 ?4523.9 ?2784.2 ?1137 ?1649.9 ?1678.9 ?1497.5 ?1664.4 ?5390.5 ?3726.1 ?3365.075 10756.1 4475.7 4207.6 3860.3 2043.6 2940 1351.5 1300.5 3400.15 7375.7 3975.55 5824.925
Whether N difference in means 2 meets 15666.075 the-8170.5th, 1103.6 the-564.6th, 2013.85-1074.5 be 1311.125-706.175 be 1345.05-630.075 be 1440.6 the 1698.45th, 3636.75 the 5548.9th, 1391.725 the 2650.775th, 1490.825 the 1874.25th, 1908.9 the 3916.025th,
In the table 30: coincidence rate is 10/10.Infer that tested sample is carcinoma of the pancreas patient's PBMC.
Embodiment 31
Operation is with reference to embodiment 1, and the result is referring to table 31.
Table 31. cross validation 15-2
Sample L48784 ?X83300 AI620827 AI494291 ?NM_020970 BF433657 ?J02959 ?BC022313 NM-000904 ?NM_004994
Whether training group median test group difference 1T mean N difference in means 2 meets ?T1?T2?T4?T5?N1?N2?N3?N4?N5 6615.2 11386.6 5007.4 6973.1 21113.1 10817.5 13886.1 16847.6 11102.05 17733.3 6631.25 7495.575 15666.075-8170.5 are not 340.3 658.7 453.5 703.5 773 782.5 1022.6 1836.3 738.25 1396.6 658.35 539 1103.6-564.6 are not 657.9 730.5 890.8 1478.2 2161.5 1772.8 1846.8 2274.3 1625.5 1949.2 323.7 939.35 2013.85 1074.5 are not 708.1 553.5 484.8 673.4 919.2 1117.8 1752.5 1,455 813.65 1,110 296.35 604.95 1311.125-706.175 are not 508.6 963.8 745.3 642.2 1460.3 1194.4 1253.1 1472.4 1079.1 1367.2 288.1 714.975 1345.05-630.075 are not 4103.5 2495.1 3504.5 2453.1 1374.7 2041.6 1114.9 1231.2 2247.35 1311.9-935.45 3139.05 1440.6 1698.45 are not 7571.2 10854.5 10304.2 8012.7 5454.1 3179.9 2481.6 3431.4 6512.65 2819.9-3692.75 9185.65 3636.75 5548.9 are not 3893.3 4182.7 5365.8 2728.2 1,524 1,808 1132.9 1,102 2268.1 1757.6-510.5 4042.5 1391.725 2650.775 are not 4635.3 1516.9 4523.9 2784.2 1,137 1649.9 1678.9 1497.5 1664.4 770.6-893.8 3365.075 1490.825 1874.25 are not 10756.1 4475.7 4207.6 3860.3 2043.6 2,940 1351.5 1300.5 3400.15 2171.3-1228.85 5824.925 1908.9 3916.025 are not
In the table 31: the rate of not meeting is 10/10.Infer the PBMC of tested sample for the normal people.
Embodiment 32
Operation is with reference to embodiment 1, and the result is referring to table 32.
Table 32. cross validation 16-1
Sample L48784 ?X83300 ?A1620827 ?A1494291 NM_020970 BF433657 ?J02959 BC022313 NM_000904 ?NM_004994
The training group ?T1?T2?T3?T5?N2?N3 6615.2 11386.6 5944.1 6973.1 10817.5 13886.1 ?340.3 ?658.7 ?496.2 ?703.5 ?782.5 ?1022.6 ?657.9 ?730.5 ?1018.2 ?1478.2 ?1772.8 ?1846.8 ?708.1 ?553.5 ?698.7 ?673.4 ?1117.8 ?1752.5 ?508.6 ?963.8 ?522.8 ?642.2 ?1194.4 ?1253.1 ?4103.5 ?2495.1 ?3439.2 ?2453.1 ?2041.6 ?1114.9 ?7571.2 ?10854.5 ?6894.9 ?8012.7 ?3179.9 ?2481.6 ?3893.3 ?4182.7 ?3176.2 ?2728.2 ?1808 ?1132.9 4635.3 1516.9 5390.5 2784.2 1649.9 1678.9 10756.1 4475.7 7375.7 3860.3 2940 1351.5
Whether median test group difference 1T mean N difference in means 2 meets ?N4?N5?T4 16847.6 17733.3 11102.05 5007.4-the 6094.65 7729.75 14821.125-7091.375th, 1,836 3 1396.6 743 453.5-289.5 549.675 1259.5-709.825th, 2274.3 1949.2 1625.5 890.8 734.7 971.2 1960.775 989.575 are 1,455 1,110 909.05 484.8-the 424.25 658.425 1358.825-700.4th, 1472.4 the 1367.2 1079.1 745.3 333.8 659.35 1321.775-662.425th, 1,231 2 1311.9 2247.35 3504.5 1257.15 3122.725 1424.9 1697.825 are 3431.4 2819.9 5163.15 10304.2 5141.05 8333.325 2978.2 5355.125 are 1,102 1757.6 2268.1 5365.8 3097.7 3495.1 1450.125 2044.975 are 1497.5 770.6 1664.4 4523.9 2859.5 3581.725 1399.225 2182.5 are 1300.5 2171.3 3400.15 4207.6 807.45 6616.95 1940.825 4676.125 are
In the table 32: coincidence rate is 10/10.Infer that tested sample is carcinoma of the pancreas patient's PBMC.
Embodiment 33
Operation is with reference to embodiment 1, and the result is referring to table 33.
Table 33. cross validation 16-2
Sample L48784 X83300 AI620827 AI494291 NM?020970 BF433657 J02959 BC0223?13 NM_000904 NM_004994
Whether training group median test group differs from 1 T mean N difference in means 2 and meets ?T1?T2?T3?T5?N2?N3?N4?N5?N1 6615.2 11386.6 5944.1 6973.1 10817.5 13886.1 16847.6 17733.3 11102.05 21113.1 10011.05 7729.75 14821.125-7091.375 are not 340.3 658.7 496.2 703.5 782.5 1022.6 1836.3 1396.6 743 773 30 549.675 1259.5-709.825 are not 657.9 730.5 1018.2 1478.2 1772.8 1846.8 2274.3 1949.2 1625.5 2161.5 536 971.2 1960.775 989.575 are not 708.1 553.5 698.7 673.4 1117.8 1752.5 1,455 1,110 909.05 919.2 10.15 658.425 1358.825-700.4 are not 508.6 963.8 522.8 642.2 1194.4 1253.1 1472.4 1367.2 1079.1 1460.3 381.2 659.35 1321.775-662.425 are not 4103.5 2495.1 3439.2 2453.1 2041.6 1114.9 1231.2 1311.9 2247.35 1374.7-872.65 3122.725 1424.9 1697.825 are not 7571.2 10854.5 6894.9 8012.7 3179.9 2481.6 3431.4 2819.9 5163.15 5454.1 290.95 8333.325 2978.2 5355.125 are 3893.3 4182.7 3176.2 2728.2 1,808 1132.9 1,102 1757.6 2268.1 1524-744.1 3495.1 1450.125 2044.975 are not 4635.3 1516.9 5390.5 2784.2 1649.9 1678.9 1497.5 770.6 1664.4 1137-527.4 3581.725 1399.225 2182.5 are not 10756.1 4475.7 7375.7 3860.3 2,940 1351.5 1300.5 2171.3 3400.15 2043.6 1356.55 6616.95 1940.825 4676.125 are not
In the table 33: the rate of not meeting is 9/10.Infer the PBMC of tested sample for the normal people.
Embodiment 34
Operation is with reference to embodiment 1, and the result is referring to table 34.
Table 34. cross validation 17-1
Sample L48784 ?X83300 ?AI620827 ?AI494291 NM_020970 ?BF433657 J02959 ?BC0223?13 ?NM_000904 ?NM_004994
Whether training group median test group difference 1T mean N difference in means 2 meets T1T2T3T5N1N3N4N5T4 6615.2 11386.6 5944.1 6973.1 21113.1 13886.1 16847.6 17733.3 12636.35 5007.4-7628.95 7729.75 17395.025 9665.275 are 340.3 658.7 496.2 703.5 773 1022.6 1836.3 1396.6 738.25 453.5 284.75 549.675 1257.125-707.45th, 657.9 730.5 1018.2 1478.2 2161.5 1846.8 2274.3 1949.2 1662.5 890.8-771.7 971.2 2057.95-1086.75th, 708.1 553.5 698.7 673.4 919.2 1752.5 1,455 1,110 813.65 484.8-the 328.85 658.425 1309.175-650.75th, 508.6 963.8 522.8 642.2 1460.3 1253.1 1472.4 1367.2 1108.45 745.3-the 363.15 659.35 1388.25-728.9th, 4103.5 2495.1 3439.2 2453.1 1374.7 1114.9 1231.2 1311.9 1913.9 3504.5 1590.6 3122.725 1258.175 1864.55 are 7571.2 10854.5 6894.9 8012.7 5454.1 2481.6 3431.4 2819.9 6174.5 10304.2 4129.7 8333.325 3546.75 4786.575 are 3893.3 4182.7 3176.2 2728.2 1,524 1132.9 1,102 1757.6 2242.9 5365.8 3122.9 3495.1 1379.125 2115.975 are 4635.3 1516.9 5390.5 2784.2 1,137 1678.9 1497.5 770.6 1597.9 4523.9 2,926 3581.725 1,271 2310.725 are 10756.1 4475.7 7375.7 3,860 3 2043.6 1351.5 1300.5 2171.3 3015.8 4207.6 1191.8 6616.95 1716.725 4900.225 are
In the table 34: coincidence rate is 10/10.Infer that tested sample is carcinoma of the pancreas patient's PBMC.
Embodiment 35
Operation is with reference to embodiment 1, and the result is referring to table 35.
Table 35. cross validation 17-2
Sample L48784 ?X83300 AI620827 ?AI494291 NM_020970 ?BF433657 J02959 BC022313 NM_000904 NM_004994
Whether training group median test group difference 1T mean N difference in means 2 meets ?T1?T2?T3?T5?N1?N3?N4?N5?N2 6615.2 11386.6 5944.1 6973.1 21113.1 13886.1 16847.6 17733.3 12636.35 10817.5-the 1818.85 7729.75 7395.025-9665.275th, 340.3 658.7 496.2 703.5 773 1022.6 1836.3 1396.6 738.25 782.5 44.25 549.675 1257.125-707.45 are not 657.9 730.5 1018.2 1478.2 2161.5 1846.8 2274.3 1949.2 1662.5 1772.8 110.3 971.2 2057.95-1086.75 are not 708.1 553.5 698.7 673.4 919.2 1752.5 1,455 1,110 813.65 1117.8 304.15 658.425 1309.175-650.75 are not 508.6 963.8 522.8 642.2 1460.3 1253.1 1472.4 1367.2 1108.45 1194.4 85.95 659.35 1388.25-728.9 are not 4103.5 2495.1 3439.2 2453.1 1374.7 1114.9 1231.2 1311.9 1913.9 2041.6 127.7 3122.725 1258.175 1864.55 are 7571.2 10854.5 6894.9 8012.7 5454.1 2481.6 3431.4 2819.9 6174.5 3179.9-2994.6 8333.325 3546.75 4786.575 are not 3893.3 4182.7 3176.2 2728.2 1,524 1132.9 1,102 1757.6 2242.9 1808-434.9 3495.1 1379.125 2115.975 are not 4635.3 1516.9 5390.5 2784.2 1,137 1678.9 1497.5 770.6 1597.9 1649.9 52 3581.725 1,271 2310.725 are 10756.1 4475.7 7375.7 3860.3 2043.6 1351.5 1300.5 2171.3 3015.8 2940-75.8 6616.95 1716.725 4900.225 are not
In the table 35: coincidence rate is 7/10.Infer the PBMC of tested sample for the normal people.
Embodiment 36
Operation is with reference to embodiment 1, and the result is referring to table 36.
Table 36. cross validation 18-1
Sample L48784 ?X83300 AI620827 ?AI494291 NM020970 ?BF433657 ?J02959 BC0223?13 ?NM?000904 ?NM?004994
Whether training group median test group difference 1T mean N difference in means 2 meets ?T1?T2?T3?T5?N1?N2?N4?N5?T4 6615.2 the 11386.6 5944.1 6973.1 21113.1 10817.5 16847.6 17733.3 11102.05 5007.4 6094.65 7729.75 16627.875-8898.125th, 340.3 658.7 496.2 703.5 773 782.5 1836.3 1396.6 738.25 453.5-the 284.75 549.675 1197.1-647.425th, 657.9 730.5 1018.2 1478.2 2161.5 1772.8 2274.3 1949.2 1625.5 890.8 734.7 971.2 2039.45-1068.25th, 708.1 553.5 698.7 673.4 919.2 1117.8 1,455 1,110 813.65 484.8-328.85 658.425 1150.5 492.075 are 508.6 963.8 522.8 642.2 1460.3 1194.4 1472.4 1367.2 1079.1 745.3-333.8 659.35 1373.575-714.225th, 4103.5 2495.1 3439.2 2453.1 1374.7 2041.6 1231.2 1311.9 2247.35 3504.5 1257.15 3122.725 1489.85 1632.875 are 7571.2 10854.5 6894.9 8012.7 5454.1 3179.9 3431.4 2819.9 6174.5 10304.2 4129.7 8333.325 3721.325 4612 are 3893.3 4182.7 3176.2 2728.2 1,524 1,808 1,102 1757.6 2268.1 5365.8 3097.7 3495.1 1547.9 1947.2 are 4635.3 1516.9 5390.5 2784.2 1,137 1649.9 1497.5 770.6 1583.4 4523.9 2940.5 3581.725 1263.75 2317.975 are 10756.1 4475.7 7375.7 3860.3 2043.6 2,940 1300.5 2171.3 3400.15 4207.6 807.45 6616.95 2113.85 4503.1 are
In the table 36: coincidence rate is 10/10.Infer that tested sample is carcinoma of the pancreas patient's PBMC.
Embodiment 37
Operation is with reference to embodiment 1, and the result is referring to table 37.
Table 37 cross validation 18-2
Sample L48784 ?X83300 AI620827 ?AI494291 ?NM_020970 ?BF433657 ?J02959 ?BC022313 NM_000904 ?NM_004994
The training group ?T1?T2?T3?T5?N1?N2?N4?N5 ?6615.2?11386.6?5944.1?6973.1?21113.1?10817.5?16847.6?17733.3 340.3 658.7 496.2 703.5 773 782.5 1836.3 1396.6 657.9 730.5 1018.2 1478.2 2161.5 1772.8 2274.3 1949.2 708.1 553.5 698.7 673.4 919.2 1117.8 1455 1110 508.6 963.8 522.8 642.2 1460.3 1194.4 1472.4 1367.2 4103.5 2495.1 3439.2 2453.1 1374.7 2041.6 1231.2 1311.9 ?7571.2?10854.5?6894.9?8012.7?5454.1?3179.9?3431.4?2819.9 3893.3 4182.7 3176.2 2728.2 1524 1808 1102 1757.6 4635.3 1516.9 5390.5 2784.2 1137 1649.9 1497.5 770.6 10756.1 4475.7 7375.7 3860.3 2043.6 2940 1300.5 2171.3
Whether median test group difference 1T mean N difference in means 2 meets N3 11102.05 13886.1 2784.05 7729.75 16627.875-8898.125 are not 738.25 1022.6 284.35 549.675 1197.1-647.425 are not 1625.5 1846.8 221.3 971.2 2039.45 1068.25 are not 813.65 1752.5 938.85 658.425 1150.5-492.075 are not 1079.1 1253.1 174 659.35 1373.575-714.225 are not 2247.35 1114.9-1132.45 3122.725 1489.85 1632.875 are not 6174.5 2481.6 3692.9 8333.325 3721.325 4612 are not 2268.1 1132.9-1135.2 3495.1 1547.9 1947.2 are not 1583.4 1678.9 95.5 3581.725 1263.75 2317.975 are 3400.15 1351.5-2048.65 6616.95 2113.85 4503.1 are not
In the table 37: the rate of not meeting is 9/10.Infer the PBMC of tested sample for the normal people.
Embodiment 38
Operation is with reference to embodiment 1, and the result is referring to table 38.
Table 38. cross validation 19-1
Sample L48784 ?X83300 AI620827 AI494291 NM_020970 ?BF433657 ?J02959 BC022313 NM_000904 ?NM_004994
Whether training group median test group difference 1T mean N difference in means 2 meets ?T1?T2?T3?T5?N1?N2?N3?N5?T4 6615.2 11386.6 5944.1 6973.1 21113.1 10817.5 13886.1 17733.3 11102.05 5007.4-the 6094.65 7729.75 15887.5-8157.75th, 340.3 658.7 496.2 703.5 773 782.5 1022.6 1396.6 738.25 453.5-the 284.75 549.675 993.675-444th, 657.9 730.5 1018.2 1478.2 2161.5 1772.8 1846.8 1949.2 1625.5 890.8-734.7 971.2 1932.575-961.375th, 708.1 553.5 698.7 673.4 919.2 1117.8 1752.5 1,110 813.65 484.8-the 328.85 658.425 1224.875-566.45th, 508.6 963.8 522.8 642.2 1460.3 1194.4 1253.1 1367.2 1079.1 745.3-333.8 659.35 1318.75-659.4th, 4103.5 2495.1 3439.2 2453.1 1374.7 2041.6 1114.9 1311.9 2247.35 3504.5 1257.15 3122.725 1460.775 1661.95 are 7571.2 10854.5 6894.9 8012.7 5454.1 3179.9 2481.6 2819.9 6174.5 10304.2 4129.7 8333.325 3483.875 4849.45 are 3893.3 4182.7 3176.2 2728.2 1,524 1,808 1132.9 1757.6 2268.1 5365.8 3097.7 3495.1 1555.625 1939.475 are 4635.3 1516.9 5390.5 2784.2 1,137 1649.9 1678.9 770.6 1664.4 4523.9 2859.5 3581.725 1309.1 2272.625 are 10756.1 4475.7 7375.7 3860.3 2043.6 2,940 1351.5 2171.3 3400.15 4207.6 807.45 6616.95 2126.6 4490.35 are
In the table 38: coincidence rate is 10/10.Infer that tested sample is carcinoma of the pancreas patient's PBMC.
Embodiment 39
Operation is with reference to embodiment 1, and the result is referring to table 39.
Table 39. cross validation 19-2
Sample L48784 ?X83300 ?AI620827 ?AI494291 ?NM_020970 BF433657 J02959 BC0223?13 ?NM_000904 ?NM_004994
The training group T1T2 ?6615.2?11386.6 ?340.3?658.7 657.9 730.5 708.1 553.5 508.6 963.8 4103.5 2495.1 7571.2 10854.5 3893.3 4182.7 4635.3 1516.9 10756.1 4475.7
Whether median test group difference 1T mean N difference in means 2 meets ?T3?T5?N1?N2?N3?N5?N4 5944.1 6973.1 21113.1 10817.5 13886.1 17733.3 11102.05 16847.6 5745.55 7729.75 15887.5-8157.75 are not 496.2703.5773782.51022.61396.6738.251836.31098.05549.675 993.675-444 is not 1018.2 1478.2 2161.5 1772.8 1846.8 1949.2 1625.5 2274.3 648.8 971.2 1932.575-961.375 are not 698.7 673.4 919.2 1117.8 1752.5 1,110 813.65 1,455 641.35 658.425 1224.875-566.45 are not 522.8 642.2 1460.3 1194.4 1,253 1 1367.2 1079.1 1472.4 393.3 659.35 1318.75 659.4 are not 3439.2 2453.1 1374.7 2041.6 1114.9 1311.9 2247.35 1231.2-1016.15 3122.725 1460.775 1661.95 are not 6894.9 8012.7 5454.1 3179.9 2481.6 2819.9 6174.5 3431.4-2743.1 8333.325 3483.875 4849.45 are not 3176.2 2728.2 1,524 1,808 1132.9 1757.6 2268.1 1102-1166.1 3495.1 1,555 625 1939.475 are not 5390.5 2784.2 1,137 1649.9 1678.9 770.6 1664.4 1497.5-166.9 3581.725 1309.1 2,272 625 are not 7375.7 3860.3 2043.6 2,940 1351.5 2171.3 3400.15 1300.5 2099.65 6616.95 2126.6 4490.35 are not
In the table 39: the rate of not meeting is 10/10.Infer the PBMC of tested sample for the normal people.
Embodiment 40
Operation is with reference to embodiment 1, and the result is referring to table 40.
Table 40. cross validation 20-1
Sample L48784 ?X83300 AI620827 AI494291 NM_020970 ?BF433657 J02959 ?BC022313 ?NM_000904 NM_004994
Whether training group median test group difference 1T mean N difference in means 2 meets ?T1?T2?T3?T5?N1?N2?N3?N4?T4 6615.2 11386.6 5944.1 6973.1 21113.1 10817.5 13886.1 16847.6 11102.05 5007.4-the 6094.65 7729.75 15666.075-7936.325th, 340.3 658.7 496.2 703.5 773 782.5 1022.6 1836.3 738.25 453.5-the 284.75 549.675 1103.6-553.925th, 657.9 730.5 1018.2 1478.2 2161.5 1772.8 1846.8 2274.3 1625.5 890.8-734.7 971.2 2013.85-1042.65th, 708.1 553.5 698.7 673.4 919.2 1117.8 1752.5 1,455 813.65 484.8-the 328.85 658.425 1311.125-652.7th, 508.6 963.8 522.8 642.2 1460.3 1194.4 1253.1 1472.4 1079.1 745.3-333.8 659.35 1345.05-685.7th, 4103.5 2495.1 3439.2 2453.1 1374.7 2041.6 1114.9 1231.2 2247.35 3504.5 1257.15 3122.725 1440.6 1682.125 are 7571.2 10854.5 6894.9 8012.7 5454.1 3179.9 2481.6 3431.4 6174.5 10304.2 4129.7 8333.325 3636.75 4696.575 are 3893.3 4182.7 3176.2 2728.2 1,524 1,808 1132.9 1,102 2268.1 5365.8 3097.7 3495.1 1391.725 2103.375 are 4635.3 1516.9 5390.5 2784.2 1,137 1649.9 1678.9 1497.5 1664.4 4523.9 2859.5 3581.725 1490.825 2090.9 are 10756.1 4475.7 7375.7 3860.3 2043.6 2,940 1351.5 1300.5 3400.15 4207.6 807.45 6616.95 1908.9 4708.05 are
In the table 40: coincidence rate is 10/10.Infer that tested sample is carcinoma of the pancreas patient's PBMC.
Embodiment 41
Operation is with reference to embodiment 1, and the result is referring to table 41.
Table 41. cross validation 20-2
Sample L48784 ?X83300 ?AI620827 AI494291 ?NM_020970 ?BF433657 ?J02959 ?BC022313 ?NM_000904 NM_004994
Whether training group median test group difference 1T mean N difference in means 2 meets T1 T2 T3 T5 N1 N2 N3 N4 N5 6615.2 11386.6 5944.1 6973.1 21113.1 10817.5 13886.1 16847.6 11102.05 17733.3 6631.25 7729.75 15666.075-7936.325 are not 340.3 658.7 496.2 703.5 773 782.5 1022.6 1836.3 738.25 1396.6 658.35 549.675 1103.6-553.925 are not 657.9 730.5 1018.2 1478.2 2161.5 1772.8 1846.8 2274.3 1625.5 1949.2 323.7 971.2 2013.85-1042.65 are not 708.1 553.5 698.7 673.4 919.2 1117.8 1752.5 1,455 813.65 1,110 296.35 658.425 1311.125-652.7 are not 508.6 963.8 522.8 642.2 1460.3 1194.4 1253.1 1472.4 1079.1 1367.2 288.1 659.35 1345.05-685.7 are not 4103.5 2495.1 3439.2 2453.1 1374.7 2041.6 1114.9 1231.2 2247.35 1311.9-935.45 3122.725 1440.6 1682.125 are not 7571.2 10854.5 6894.9 8012.7 5454.1 3179.9 2481.6 3431.4 6174.5 2819.9 3354.6 8333.325 3636.75 4696.575 are not 3893.3 4182.7 3176.2 2728.2 1,524 1,808 1132.9 1,102 2268.1 1757.6 510.5 3495.1 1391.7225 2103.375 are not 4635.3 1516.9 5390.5 2784.2 1,137 1649.9 1678.9 1497.5 1664.4 770.6 893.8 3581.725 1490.825 2090.9 are not 10756.1 4475.7 7375.7 3860.3 2043.6 2,940 1351.5 1300.5 3400.15 2171.3-1228.85 6616.95 1908.9 4708.05 are not
In the table 41: the rate of not meeting is 10/10.Infer the PBMC of tested sample for the normal people.
Embodiment 42
Operation is with reference to embodiment 1, and the result is referring to table 42.
Table 42. cross validation 21-1
Sample L48784 ?X83300 ?AI620827 AI494291 NM_020970 BF433657 ?J02959 BC022313 NM_000904 ?NM_004994
Training group median test group differs from 1 T1 T2 T3 T4 N2 N3 N4 N5 T5 6615.211386.65944.15007.410817.513886.116847.617733.311102.056973.1-4128.95 340.3 658.7 496.2 453.5 782.5 1022.6 1836.3 1396.6 720.6 703.5 -17.1 657.9 730.5 1018.2 890.8 1772.8 1846.8 2274.3 1949.2 1395.5 1478.2 82.7 708.1 553.5 698.7 484.8 1117.8 1752.5 1455 1110 909.05 673.4 -235.65 508.6 963.8 522.8 745.3 1194.4 1253.1 1472.4 1367.2 1079.1 642.2 -436.9 4103.5 2495.1 3439.2 3504.5 2041.6 1114.9 1231.2 1311.9 2268.35 2453.1 184.75 7571.2 10854.5 6894.9 10304.2 3179.9 2481.6 3431.4 2819.9 5163.15 8012.7 2849.55 3893.3 4182.7 3176.2 5365.8 1808 1132.9 1102 1757.6 2492.1 2728.2 236.1 4635.3 1516.9 5390.5 4523.9 1649.9 1678.9 1497.5 770.6 1664.4 2784.2 1119.8 10756.1 4475.7 7375.7 4207.6 2940 1351.5 1300.5 2171.3 3573.8 3860.3 286.5
Whether T mean N difference in means 2 meets 7238.32514821.125-7582.8 be 487.175 1259.5-772.325th, 824.35 1960.775-1136.425 are not 611.2751358.825-747.55 be 685.1251321.775-636.65 be 3385.575 1424.9 1960.675 are 8906.2 2978.2 5928 are 4154.5 1450.125 2704.375 are 4016.651399.2252617.425 be 6703.775 1940.825 4762.95 are
In the table 42: coincidence rate is 9/10.Infer that tested sample is carcinoma of the pancreas patient's PBMC.
Embodiment 43
Operation is with reference to embodiment 1, and the result is referring to table 43.
Table 43. cross validation 21-2
Sample L48784 X83300 AI620827 AI494291 NM_020970 BF433657 ?J02959 ?BC022313 ?NM_000904 ?NM_004994
Whether training group median test group difference 1T mean N difference in means 2 meets T1 T2 T3 T4 N2 N3 N4 N5 N1 6615.2 11386.6 5944.1 5007.4 10817.5 13886.1 16847.6 17733.3 11102.05 21113.1 10011.05 7238.325 14821.125-7582.8 are not 340.3 658.7 496.2 453.5 782.5 1022.6 1836.3 1396.6 720.6 773 52.4 487.175 1259.5-772.325 are not 657.9 730.5 1018.2 890.8 1772.8 1846.8 2274.3 1949.2 1395.5 2161.5 766 824.35 1960.775-1136.42 are not 708.1 553.5 698.7 484.8 1117.8 1752.5 1,455 1,110 909.05 919.2 10.15 611.275 1358.825-747.55 are not 508.6 963.8 522.8 745.3 1194.4 1253.1 1472.4 1367.2 1079.1 1460.3 381.2 685.125 1321.775-636.65 are not 4103.5 2495.1 3439.2 3504.5 2041.6 1114.9 1231.2 1311.9 2268.35 1374.7-893.65 3385.575 1424.9 1960.675 are not 7571.2 10854.5 6894.9 10304.2 3179.9 2481.6 3431.4 2819.9 5163.15 5454.1 290.95 8906.2 2978.2 5928 are 3893.3 4182.7 3176.2 5365.8 1,808 1132.9 1,102 1757.6 2492.1 1,524 968.1 4154.5 1450.125 2704.375 are not 4635.3 1516.9 5390.5 4523.9 1649.9 1678.9 1497.5 770.6 1664.4 1137-527.4 4016.65 1399.225 2617.425 are not 10756.1 4475.7 7375.7 4207.6 2,940 1351.5 1300.5 2171.3 3573.8 2043.6 1530.2 6703.775 1940.825 4762.95 are not
In the table 43: the rate of not meeting is 9/10.Infer the PBMC of tested sample for the normal people.
Embodiment 44
Operation is with reference to embodiment 1, and the result is referring to table 44.
Table 44. cross validation 22-1
Sample L48784 ?X83300 ?AI620827 ?AI494291 ?NM_020970 BF433657 J02959 ?BC022313 ?NM_000904 ?NM_004994
The training group ?T1?T2?T3?T4?N1 ?6615.2?11386.6?5944.1?5007.4?21113.1 ?340.3?658.7?496.2?453.5?773 657.9 730.5 1018.2 890.8 2161.5 708.1 553.5 698.7 484.8 919.2 508.6 963.8 522.8 745.3 1460.3 4103.5 2495.1 3439.2 3504.5 1374.7 7571.2 10854.5 6894.9 10304.2 5454.1 ?3893.3 ?4182.7 ?3176.2 ?5365.8 ?1524 ?4635.3 ?1516.9 ?5390.5 ?4523.9 ?1137 10756.1 4475.7 7375.7 4207.6 2043.6
Whether median test group difference 1T mean N difference in means 2 meets ?N3?N4?N5?T5 13886.116847.617733.312636.356973.1-5663.257238.32517395 .025-10156.7 is 1022.6 1836.3 1396.6 715.85 703.5-12.35 487.175 1257.125-769.95th, 1846.8 2274.3 1949.2 1432.5 1478.2 45.7 824.35 2057.95-1233.6 are not 1752.5 1,455 1,110 813.65 673.4-the 140.25 611.275 1309.175-697.9th, 1253.1 1472.4 1367.2 1108.45 642.2-the 466.25 685.125 1388.25-703.125th, 1114.9 1231.2 1311.9 1934.9 2453.1 518.2 3385.575 1258.175 2127.4 are 2481.6 3431.4 2819.9 6174.5 8012.7 1838.2 8906.2 3546.75 5359.45 are 1132.9 1,102 1757.6 2466.9 2728.2 261.3 4154.5 1379.125 2775.375 are 1678.9 1497.5 770.6 1597.9 2784.2 1186.3 4016.65 1,271 2745.65 are 1351.5 1300.5 2171.3 3189.45 3860.3 670.85 6703.775 1716.725 4987.05 are
In the table 44: coincidence rate is 9/10.Infer that tested sample is carcinoma of the pancreas patient's PBMC.
Embodiment 45
Operation is with reference to embodiment 1, and the result is referring to table 45.
Table 45. cross validation 22-2
Sample L48784 ?X83300 AI620827 ?AI494291 NM_020970 BF433657 ?J02959 ?BC022313 NM_000904 NM_004994
Whether training group median test group difference 1T mean N difference in means 2 meets ?T1?T2?T3?T4?N1?N3?N4?N5?N2 6615.211386.65944.15007.421113.113886.116847.617733.3126 36.3510817.5-1818.857238.32517395.025-10156.7 is 340.3 658.7 496.2 453.5 773 1022.6 1836.3 1396.6 715.85 782.5 66.65 487.175 1257.125-769.95 are not 657.9 730.5 1018.2 890.8 2161.5 1846.8 2274.3 1949.2 1432.5 1772.8 340.3 824.35 2057.95 1233.6 are not 708.1 553.5 698.7 484.8 919.2 1752.5 1,455 1,110 813.65 1117.8 304.15 611.275 1309.175-697.9 are not 508.6 963.8 522.8 745.3 1460.3 1253.1 1472.4 1367.2 1108.45 1194.4 85.95 685.125 1388.25-703.125 are not 4103.5 2495.1 3439.2 3504.5 1374.7 1114.9 1231.2 1311.9 1934.9 2041.6 106.7 3385.575 1258.175 2127.4 are 7571.2 10854.5 6894.9 10304.2 5454.1 2481.6 3431.4 2819.9 6174.5 3179.9-2994.6 8906.2 3546.75 5359.45 are not 3893.3 4182.7 3176.2 5365.8 1,524 1132.9 1,102 1757.6 2466.9 1808-658.9 4154.5 1379.125 2775.375 are not 4635.3 1516.9 5390.5 4523.9 1,137 1678.9 1497.5 770.6 1597.9 1649.9 52 4016.65 1,271 2745.65 are 10756.1 4475.7 7375.7 4207.6 2043.6 1351.5 1,300 5 2171.3 3189.45 2940-249.45 6703.775 1716.725 4,987 05 are not
In the table 45: the rate of not meeting is 7/10.Infer the PBMC of tested sample for the normal people.
Embodiment 46
Operation is with reference to embodiment 1, and the result is referring to table 46.
Table 46. cross validation 23-1
Sample L48784 X83300 AI620827 AI494291 NM_020970 BF433657 J02959 ?BC022313 NM_000904 NM_004994
Whether training group median test group difference 1T mean N difference in means 2 meets ?T1?T2?T3?T4?N1?N2?N4?N5?T5 6615.2 11386.6 5944.1 5007.4 21113.1 10817.5 16847.6 17733.3 11102.05 6973.1-the 4128.95 7238.325 16627.875-9389.55th, 340.3 658.7 496.2 453.5 773 782.5 1836.3 1396.6 715.85 703.5-12.35 487.175 1197.1-709.925th, 657.9 730.5 1018.2 890.8 2161.5 1772.8 2274.3 1949.2 1395.5 1478.2 82.7 824.35 2039.45-1215.1 are not 708.1 553.5 698.7 484.8 919.2 1117.8 1,455 1,110 813.65 673.4-140.25 611.275 1150.5 539.225 are 508.6 963.8 522.8 745.3 1460.3 1194.4 1472.4 1367.2 1079.1 642.2-436.9 685.125 1373.575 688.45 are 4103.5 2495.1 3439.2 3504.5 1374.7 2041.6 1231.2 1311.9 2268.35 2453.1 184.75 3385.575 1489.85 1895.725 are 7571.2 10854.5 6894.9 10304.2 5454.1 3179.9 3431.4 2819.9 6174.5 8012.7 1838.2 8906.2 3721.325 5184.875 are 3893.3 4182.7 3176.2 5365.8 1,524 1,808 1,102 1757.6 2492.1 2728.2 236.1 4154.5 1547.9 2606.6 are 4635.3 1516.9 5390.5 4523.9 1,137 1649.9 1497.5 770.6 1583.4 2784.2 1200.8 4016.65 1263.75 2752.9 are 10756.1 4475.7 7375.7 4207.6 2043.6 2,940 1300.5 2171.3 3573.8 3860.3 286.5 6703.775 2113.85 4589.925 are
In the table 46: coincidence rate is 9/10.Infer that tested sample is carcinoma of the pancreas patient's PBMC.
Embodiment 47
Operation is with reference to embodiment 1, and the result is referring to table 47.
Table 47. cross validation 23-2
Sample L48784 X83300 AI620827 AI494291 ?NM_020970 BF433657 J02959 BC022313 NM_000904 NM004994
Training group median test group difference 1T mean N difference in means 2 T1 T2 T3 T4 N1 N2 N4 N5 N3 6615.2 11386.6 5944.1 5007.4 21113.1 10817.5 16847.6 17733.3 11102.05 13886.1 2784.05 7238.325 16627.875 -9389.55 340.3 668.7 496.2 453.5 773 782.5 1836.3 1396.6 715.85 1022.6 306.75 487.175 1197.1 709.925 657.9 730.5 1018.2 890.8 2161.5 1772.8 2274.3 1949.2 1395.5 1846.8 451.3 824.35 2039.45 -1215.1 708.1 553.5 698.7 484.8 919.2 1117.8 1455 1110 813.65 1752.5 938.85 611.275 1150.5 539.225 ?508.6 ?963.8 ?522.8 ?745.3 ?1460.3 ?1194.4 ?1472.4 ?1367.2 ?1079.1 ?1253.1 ?174 ?685.125 ?1373.575 ?-688.45 4103.5 2495.1 3439.2 3504.5 1374.7 2041.6 1231.2 1311.9 2268.35 1114.9 -1153.45 3385.575 1489.85 1895.725 7571.2 10854.5 6894.9 10304.2 5454.1 3179.9 3431.4 2819.9 6174.5 2481.6 -3692.9 8906.2 3721.325 5184.875 3893.3 4182.7 3176?2 5365.8 1524 1808 1102 1757.6 2492.1 1132.9 -1359.2 4154.5 1547.9 2606.6 4635.3 1516.9 5390.5 4523.9 1137 1649.9 1497.5 770.6 1583.4 1678.9 95.5 4016.65 1263.75 2752.9 ?10756.1 ?4475.7 ?7375.7 ?4207.6 ?2043.6 ?2940 ?1300.5 ?2171.3 ?3573.8 ?1351.5 ?-2222.3 ?6703.775 ?2113.85 ?4589.925
Whether meet Not Not Not Not Not Not Not Not Be Not
In the table 47: the rate of not meeting is 9/10.Infer the PBMC of tested sample for the normal people.
Embodiment 48
Operation is with reference to embodiment 1, and the result is referring to table 48.
Table 48. cross validation 24-1
Sample L48784 X83300 ?AI620827 ?AI494291 NM_020970 ?BF433657 ?J02959 ?BC022313 ?NM_000904 NM_004994
Whether training group median test group differs from 1 mean N difference in means 2 and meets ?T1?T2?T3?T4?N1?N2?N3?N5?T5 6615.2 11386.6 5944.1 5007.4 21113.1 10817.5 13886.1 17733.3 11102.05 6973.1-the 4128.95 7238.325 15887.5-8649.175th, 340.3 658.7 496.2 453.5 773 782.5 1022.6 1396.6 715.85 703.5-12.35 487.175 993.675-506.5th, 657.9 730.5 1018.2 890.8 2161.5 1772.8 1846.8 1949.2 1395.5 1478.2 82.7 824.35 1932.575-1108.225 are not 708.1 553.5 698.7 484.8 919.2 1117.8 1752.5 1,110 813.65 673.4-the 140.25 611.275 1224.875-613.6th, 508.6 963.8 522.8 745.3 1460.3 1194.4 1253.1 1367.2 1079.1 642.2-436.9 685.125 1318.75-633.625th, 4103.5 2495.1 3439.2 3504.5 1374.7 2041.6 1114.9 1311.9 2268.35 2453.1 184.75 3385.575 1460.775 1924.8 are 7571.2 10854.5 6894.9 10304.2 5454.1 3179.9 2481.6 2819.9 6174.5 8012.7 1838.2 8906.2 3483.875 5422.325 are 3893.3 4182.7 3176.2 5365.8 1,524 1,808 1132.9 1757.6 2492.1 2728.2 236.1 4154.5 1555.625 2598.875 are 4635.3 1516.9 5390.5 4523.9 1,137 1649.9 1678.9 770.6 1664.4 2784.2 1119.8 4016.65 1309.1 2707.55 are 10756.1 4475.7 7375.7 4207.6 2043.6 2,940 1351.5 2171.3 3573.8 3860.3 286.5 6703.775 2126.6 4577.175 are
In the table 48: coincidence rate is 9/10.Infer that tested sample is carcinoma of the pancreas patient's PBMC.
Embodiment 49
Operation is with reference to embodiment 1, and the result is referring to table 49.
Table 49. cross validation 24-2
Sample L48784 ?X83300 ?AI620827 AI494291 ?NM_020970 ?BF433657 ?J02959 ?BC022313 ?NM_000904 ?NM_004994
The training group ?T1?T2?T3?T4?N1?N2?N3?N5 ?6615.2?11386.6?5944.1?5007.4?21113.1?10817.5?13886.1?17733.3 ?340.3?658.7?496.2?453.5?773?782.5?1022.6?1396.6 657.9 730.5 1018.2 890.8 2161.5 1772.8 1846.8 1949.2 708.1 553.5 698.7 484.8 919.2 1117.8 1752.5 1110 508.6 963.8 522.8 745.3 1460.3 1194.4 1253.1 1367.2 4103.5 2495.1 3439.2 3504.5 1374.7 2041.6 1114.9 1311.9 7571.2 10854.5 6894.9 10304.2 5454.1 3179.9 2481.6 2819.9 3893.3 4182.7 3176.2 5365.8 1524 1808 1132.9 1757.6 4635.3 1516.9 5390.5 4523.9 1137 1649.9 1678.9 770.6 10756.1 4475.7 7375.7 4207.6 2043.6 2940 1351.5 2171.3
Whether median test group difference 1T mean N difference in means 2 meets N4 11102.0516847.65745.557238.32515887.5-8649.175 not 715.85 1836.3 1120.45 487.175 993.675-506.5 are not 1395.5 2274.3 878.8 824.35 1932.575-1108.225 are not 813.65 1,455 641.35 611.275 1224.875-613.6 are not 1079.1 1472.4 393.3 685.125 1318.75-633.625 are not 2268.35 1231.2-1037.15 3385.575 1460.775 1924.8 are not 6174.53431.4-2743.18906.23483.8755422.325 not 2492.11102-1390.14154.51555.6252598.875 not 1664.41497.5-166.94016.651309.12707.55 not 3573.8 1300.5-2273.3 6703.775 2126.6 4577.175 are not
In the table 49: the rate of not meeting is 10/10.Infer the PBMC of tested sample for the normal people.
Embodiment 50
Operation is with reference to embodiment 1, and the result is referring to table 50.
Table 50. cross validation 25-1
Sample L48784 X83300 AI620827 AI494291 NM_020970 BF433657 J02959 BC0223?13 NM_000904 NM_004994
Whether training group median test group difference 1T mean N difference in means 2 meets T1?T2?T3?T4?N1?N2?N3?N4?T5 6615.2 11386.6 5944.1 5007.4 21113.1 10817.5 13886.1 16847.6 11102.05 6973.1-the 4128.95 7238.325 15666.075-8427.75th, 340.3 658.7 496.2 453.5 773 782.5 1022.6 1836.3 715.85 703.5-12.35 487.175 1103.6-616.425th, 657.9 730.5 1018.2 890.8 2161.5 1772.8 1846.8 2274.3 1395.5 1478.2 82.7 824.35 2013.85-1189.5 are not 708.1 553.5 698.7 484.8 919.2 1117.8 1752.5 1,455 813.65 673.4-the 140.25 611.275 1311.125-699.85th, 508.6 963.8 522.8 745.3 1460.3 1194.4 1253.1 1472.4 1079.1 642.2-436.9 685.125 1345.05-659.925th, 4103.5 2495.1 3439.2 3504.5 1374.7 2041.6 1114.9 1231.2 2268.35 2453.1 184.75 3385.575 1440.6 1944.975 are 7571.2 10854.5 6894.9 10304.2 5454.1 3179.9 2481.6 3431.4 6174.5 8012.7 1838.2 8906.2 3636.75 5269.45 are 3893.3 4182.7 3176.2 5365.8 1,524 1,808 1132.9 1,102 2492.1 2728.2 236.1 4154.5 1391.725 2762.775 are 4635.3 1516.9 5390.5 4523.9 1,137 1649.9 1678.9 1497.5 1664.4 2784.2 1119.8 4016.65 1490.825 2525.825 are 10756.1 4475.7 7375.7 4207.6 2043.6 2,940 1351.5 1300.5 3573.8 3860.3 286.5 6703.775 1908.9 4794.875 are
In the table 50: coincidence rate is 9/10.Infer that tested sample is carcinoma of the pancreas patient's PBMC.
Embodiment 51
Operation is with reference to embodiment 1, and the result is referring to table 51.
Table 51. cross validation 25-2
Sample L48784 X83300 AI620827 AI494291 NM 020970 BF433657 J02959 BC022313 NM_000904 NM_004994
The training group T1T2 6615.2 11386.6 340.3 658.7 657.9 730.5 708.1 553.5 508.6 963.8 4103.5 2495.1 7571.2 10854.5 3893.3 4182.7 4635.3 1516.9 10756.1 4475.7
Whether median test group difference 1T mean N difference in means 2 meets T3T4N1N2N3N4N5 5944.1 5007.4 21113.1 10817.5 13886.1 16847.6 11102.05 17733.3 6631.25 7238.325 15666.075-8427.75 are not 496.2 453.5 773 782.5 1022.6 1836.3 715.85 1396.6 680.75 487.175 1103.6-616.425 are not 1018.2 890.8 2161.5 1772.8 1846.8 2274.3 1395.5 1949.2 553.7 824.35 2013.85-1189.5 are not 698.7 484.8 919.2 1117.8 1752.5 1,455 813.65 1,110 296.35 611.275 1311.125-699.85 are not 522.8 745.3 1460.3 1194.4 1253.1 1472.4 1079.1 1367.2 288.1 685.125 1345.05-659.925 are not 3439.2 3504.5 1374.7 2041.6 1114.9 1231.2 2268.35 1311.9-956.45 3385.575 1440.6 1944.975 are not 6894.9 10304.2 5454.1 3179.9 2481.6 3431.4 6174.5 2819.9-3354.6 8906.2 3636.75 5269.45 are not 3176.2 5365.8 1,524 1,808 1132.9 1,102 2492.1 1757.6 734.5 4154.5 1391.725 2762.775 are not 5390.5 4523.9 1,137 1649.9 1678.9 1497.5 1664.4 770.6 893.8 4016.65 1490.825 2525.825 are not 7375.7 4207.6 2043.6 2,940 1351.5 1300.5 3573.8 2171.3-1402.5 6703.775 1908.9 4794.875 are not
In the table 51: the rate of not meeting is 10/10.Infer the PBMC of tested sample for the normal people.
The sequence that the present invention relates to
< 110>Zhejiang University
The assortment of genes and the application of < 120>one groups of screening ductal adenocarcinoma of pancreass
<160>10
<170>Patent?In?VErsion?2.1
<210>1
<211>220
< 213>people
<400>1
L48784
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61?atgccagtgc?agaagcagac?ccgtgccggc?cagcgcacca?ggttcaaggt?acccggctgt
121?cctgggaggg?ggctgcgctg?ggcttgccgg?gactctctcg?gctctgcata?gttgcacttg
181?gcttcacccg?tgtgactttc?gtaacgggga?gagagagaaa
<210>2
<211>1295
< 213>people
<400>2
X83300
1?gtgaaagagc?aggttctgct?tctttggtgt?agtcctgaag?cttcctaaga?aacttcacat
61?caggtgatgg?ataggagcaa?ccctgtaaaa?ccagccttag?actatttttc?aaacaggctg
121?gtgaattacc?agatctccgt?caagtgcagt?aaccagttca?agttggaagt?gtgtcttttg
181?aatgcagaaa?acaaagtcgt?ggacaaccag?gctgggaccc?agggccagct?gaaggtgctg
241?ggtgccaacc?tctggtggcc?gtacctgatg?cacgaacacc?ccgcctacct?gtactcgtgg
301?gaggatggtg?attgctcaca?ccaaagcctt?ggacccctcc?cagcctgtga?cctttgtgac
361?caactccacc?tacgcagcag?acaagggggc?tctgtatgtg?gatgtgatcc?gtgtgaacgg
421?ctactactct?tggtatcgca?actacgggca?cctggagttg?attcagctgc?agctggccgc
481?ccagtttgag?aattggtgta?agacatcaca?atcccattat?tcagagcgcg?tatggagtgg
541?aaacgcttgt?agggtttcac?caggtaagcg?gtgttgaact?ttctgcttgt?gtattctctc
601?tgggcagaga?tgccacttgc?ctcccccacc?atgccatctc?tgaagaatat?tacagaccat
661?tttggagcat?ggtgaataag?aaattttcac?cttaggagtt?cagttgaata?gtcattttta
721?tatttgtgac?tgcaagtcac?tcttaggggc?tgtacttcct?tagtactggt?agcattatta
781?tccaatggac?ttttatagct?ttcattaggt?tttcttttgt?ttttgttctt?taaagaacgt
841?tttacttatc?ttagtatttc?atttttcatc?tatattatga?ggcagtaaga?gtcttctgtt
901?tttccaaagt?tgagactgct?ttatatttat?ttcgtattgt?ctacagctgt?agtgttcaat
961?acattagcca?ctagccacat?gtggttattt?aaataagata?aaataaaaat?tggccgggcg
1021?tggtgggtca?cgccggtaat?cccagcactt?tgggaggccg?aggcgggcag?atcattaggt
1081?caggagatcg?agaccatcct?tactaagacg?gtgaaccccc?atctctatta?aaaatacaaa
1141?aaattagccg?ggcgtggtgg?cgggcgcctg?cagtcccagc?tactcaggag?gctgaggcag
1201?gagaatggcg?tgaacctggg?aggcagagtt?tgcagtgagc?cgagatggcg?ccactgcact
1261?ccagcctggg?gacagagcga?gactccatct?caaaa
<210>3
<211>776
< 213>people
<400>3
AI620827
1?ttacacttgt?tagatcttta?ttcattatag?attctccaat?gatttgcaat?ggttgtagcg
61?ttactgaaga?ctttgtgaca?atcattacat?tagtcaagtt?tccctacacc?atgaactgcc
121?tgatggtgaa?taagtgttga?ctgcttgcta?aaggctttgc?cacactcatt?acacttgtaa
181?ggtttctctc?cagtgtgaag?tccagtatgt?tgtttcaggt?gtgaatcact?cccaaaagtc
241?ttgtcacaaa?ccttacattt?gtatggtttc?tttccaggat?gaattctcct?atgtctttta
301?aggtgtgatt?tgcgactgaa?aactttgtca?catgtttcac?atttgtaaag?tttctcccca
361?gcatgaattc?tatgatgaag?tgaaagttgt?gattgttgat?taaaagcctt?cccacattca
421?ttacacttgt?aaggtttctc?tgcagtgtga?attctgggat?gtcttgccag?gtatgaatta
481?cgcacgaaag?ccttgtcaca?accgtacatt?tgtaagattt?ctctccagta?tgaagtctac
541?natggtgaac?caagggatgg?cttggggact?gaagtccttg?cacactcatt?acacttgtaa
601?gtttctcaac?cactatgagn?ctacgatgga?aaggaagatg?acccttggac?tgaggcttgg
661?ttgactcatt?taactggaag?gttctaacac?tatgaactct?gaatggctgc?aagatgacct
721?aagaacgagc?ttgctgacta?atacacttga?agtctttccc?ttgaaaacta?atggtg
<210>4
<211>447
< 213>people
<400>4
AI494291
1?ttttttttaa?gaaaattcag?taattctcca?ttgaacatct?actacatatc?caactctgtg
61?ctaaggtatg?ctgtggaaaa?gtcaaggaag?aatgtatgag?acacatccct?gcactcccag
121?gagtttataa?tttcggagga?gatgcaaacg?tggacccatt?ttaatgtaac?aaaagataca
181?ctgtgttaac?agccaaaatg?gaggcctgta?cagagtactg?gggcaacctg?gaaatctggt
241?tttaggatac?actgcattca?aagcaatttg?cttttttctt?caacatttct?tgagcacctg
301?ttatgtgcaa?agcaaggagt?caggtgcctt?tctggaaaac?gaaataaaca?cggcactgtc
361?tctgtttaca?actgcccatt?taacaaacat?ttcttgaaca?tctactacat?gtgatgcatt
421?gagctcaacc?ttaggaattg?cagatgg
<210>5
<211>2418
< 213>people
<400>5
NM_020970
1?gtgcagaaag?caccgtcttt?tactagaaga?agacaataaa?atgttggtca?atgaactgaa
61?tcattcgaaa?gaaaaagaat?gccaatatga?aaaagagaaa?gcagaaagag?aagtagctgt
121?gagacagctt?caacaaaaac?gagatgatgt?cttaaacaaa?ggatcagcaa?caaaagctct
181?gctggatgct?tcatcgcgtc?actgcaccta?tttagaaaat?gggatgcagg?attcaaggaa
241?gaaattagac?cagatgagaa?gtcaatttca?agaaatacag?gatcaactta?cagctactat
301?aagatgtact?aaggagatgg?atggcgacac?acaaaagctt?gaagtagaac?atgtgatgat
361?gagaaaaatt?attaaaaaac?aggatgacca?aattgagcgg?cttgagaaaa?tcctgcagca
421?ttcaagtttg?atgctgcagg?tgtttgagag?ctagatgaag?atatggacat?ttctggaatt
481?aagagccccc?tctacattga?gcacaagaca?gtgacgaatc?cacattcttc?agccagaaag
541?tcacctctga?gagccaaatg?tctgactata?gaaaggagag?tcaaagaatg?ataacgacat
601?ctcaggaaaa?ttgtgaaaat?aatcagcagc?cggatgtgga?taaccctttc?aaatttctcc
661?tggggcctcc?aaccaccccc?actggtgttc?tcctgctgac?agtgatttca?attttttctg
721?ggctggagct?ccctgatgga?ggggcaggcc?accatctttg?ctgtttttgc?aacttagcca
781?cttcagcctt?cagtctttgg?agtgtccaag?gagaccaggg?ggtgatgtgg?accctcagca
841?tagcacagct?gctctataaa?gatgaggcca?gactgctttt?ttgagcactt?tcccaatgcc
901?attcctcctc?actgggcaaa?acctccaaac?tggggtctcc?agccacctcc?tacaggtgtg
961?tttgggccag?caacaagttc?attcatacct?ccctagggca?aagcttccaa?agggagcggt
1021?aggctgccat?ctttgctgtt?tcacaggctt?cactgatgat?aactccaggt?actggaaaat
1081?ctgaggctac?tagagactgg?agcgggccct?gggcatactg?cagcagccct?atggaaaagt
1141?ggccagactg?ttacctgggt?tcccattcct?atatcttctc?actaggcaag?tcttgcaggc
1201?ctgggcctct?acctaacccc?ccctaccaga?actgttgagc?cagtagcaac?tcagccactc
1261?cctggagaga?gcctccaggg?gcaactgaaa?gcctctctgc?cactgcttct?gcagtggaac
1321?tgtccttgct?accctcagac?tgatgaagga?gctaacaccc?ttatctacac?cttcaacaag
1381?ctttaattga?ccaaagccca?tctctcatgg?gttctacaca?ctccccactg?ctcatgacag
1441?ggaacccctg?gattggcccc?cacagcacga?attctccatc?ctgattgctg?attgcagtaa
1501?acagttgctg?tattctccag?gggtggtgga?actctgagga?gacaaacaaa?agacccttgg
1561?ctacaaccac?tactaatgtc?ccttcctctt?ctgcctcaaa?gttaggaaag?aaatataaac
1621?actgagattg?ccccagagct?gcagtgggca?gcctaggagt?gccaagccat?gacctacagc
1681?cagcactcaa?gggggagaga?agcacatttt?cagatcattg?agagggaaca?tggctgcaac
1741?tgtaaggaaa?cataggggag?ccacatgacc?aagcaagagt?ctaccaactg?accagtaagc
1801?ccaagtgcca?cctactggat?cacatcccaa?agcttcagca?tcaaaaatac?cctactaata
1861?tactcccctc?tgaaaccaga?aatgagaagt?cagcttcaaa?taaagaccct?gcacaaagcc
1921?tcagcctggt?gaaaacatcc?gaaaataagt?ctacggactg?tactcaatct?acactgcaat
1981?taaaggaaaa?cccataggtg?gaaatgagaa?gaaaccaatg?caagaactcc?agtaactcaa
2041?atggcctctg?tgtcatatgt?ccttctaaca?accacaccag?ttctccaaca?agagttctta
2101?acctggatga?actgtctgga?attacataaa?tataattcag?aatatggata?ggaaaaaaaa
2161?tcatcaagac?tcaggagaat?ggcaaaaccc?aatccaagga?aaataagaat?aacagtaaag
2221?tgttacagga?gctgaaggat?aaagtagctg?gtataataaa?aaagaaccta?accgatctga
2281?aagcgccgaa?gaacacaata?caagaattcc?acaatgcaat?cacaagtatt?aacagcagaa
2341?aaaaaaacct?gaggaacgaa?tctcagaact?tgaagattgg?ttctctaaaa?taagatggac
2401 aaaaaaaaaa aaaaaaaa < 210>6 < 211>549 < 213>people < 400>6
BF433657
1?ttttttgttt?tcccttgact?ttatttatct?tcataagtca?caaaatgtga?gtgcagagat
61?aaatgtctgt?gtgcatgtgc?cctgagcaca?cagggtggca?taactcggca?cactcataat
121?gacacagccg?ttcacccagc?cacagatagt?gacagggcac?acatggcgac?acccacatgt
181?acggagataa?atctccccca?ccatgacatg?ggtagacaga?aaacacgccg?cagtatactc
241?tagtatgttt?acacaaacag?ggagacaggc?ccgtgcaatg?catcacca?acacccacac
301?tcagagtgac?atctgctgga?ggtgctcaga?cacagccacc?caccgtgaca?tgccgagact
361?cacatatgtc?acatgacaca?ggcatgcatg?ccacattcac?tgtgactctc?agtcctattc
421?attcatcacc?tttctgggag?atacactgaa?atgtccaccc?tttgcaaaat?gcacacacac
481?gcgcacgcac?acacgcacac?acacgaacac?acgcgcacac?acgcacacac?acgcacgcag
541?gtgtacaca
<210>7
<211>1910
< 213>people
<400>7
J02959
1?gggcgctgtg?ctgtgtgatc?ccccagagcc?atgcccgaga?tagtggatac?ctgttcgttg
61?gcctctccgg?cttccgtctg?ccggaccaag?cacctgcacc?tgcgctgcag?cgtcgacttt
121?actcgccgga?cgctgaccgg?gactgctgct?ctcacggtcc?agtctcagga?ggacaatctg
181?cgcagcctgg?ttttggatac?aaaggacctt?acaatagaaa?aagtagtgat?caatggacaa
241?gaagtcaaat?atgctcttgg?agaaagacaa?agttacaagg?gatcgccaat?ggaaatctct
301?cttcctatcg?ctttgagcaa?aaatcaagaa?attgttatag?aaatttcttt?tgagacctct
361?ccaaaatctt?ctgctctcca?gtggctcact?cctgaacaga?cttctgggaa?ggaacaccca
421?tatctcttta?gtcagtgcca?ggccatccac?tgcagagcaa?tccttccttg?tcaggacact
481?ccttctgtga?aattaaccta?tactgcagag?gtgtctgtcc?ctaaagaact?ggtggcactt
541?atgagtgcta?ttcgtgatgg?agaaacacct?gacccagaag?acccaagcag?gaaaatatac
601?aaattcatcc?aaaaagttcc?aataccctgc?tacctgattg?ctttagttgt?tggagcttta
661?gaaagcaggc?aaattggccc?aagaactttg?gtgtggtctg?agaaagagca?ggtggaaaag
721?tctgcttatg?agttttctga?gactgaatct?atgcttaaaa?tagcagaaga?tctgggagga
781?ccgtatgtat?ggggacagta?tgacctattg?gtcctgccac?catccttccc?ttatggtggc
841?atggagaatc?cttgccttac?ttttgtaact?cctactctac?tggcaggcga?caagtcactc
901?tccaatgtca?ttgcacatga?aatatctcat?agctggacag?ggaatctagt?gaccaacaaa
961?acttgggatc?acttttggtt?aaatgaggga?catactgtgt?acttggaacg?ccacatttgc
1021?ggacgattgt?ttggtgaaaa?gttcagacat?tttaatgctc?tgggaggatg?gggagaacta
1081?cagaattcgg?taaagacatt?tggggagaca?catcctttca?ccaaacttgt?ggttgatctg
1141?acagatatag?accctgatgt?agcttattct?tcagttccct?atgagaaggg?ctttgcttta
1201?cttttttacc?ttgaacaact?gcttggagga?ccagagattt?tcctaggatt?cttaaaagct
1261?tatgttgaga?agttttccta?taagagcata?actactgatg?actggaagga?tttcctgtat
1321?tcctatttta?aagataaggt?tgatgttctc?aatcaagttg?attggaatgc?ctggctctac
1381?tctcctggac?tgcctcccat?aaagcccaat?tatgatatga?ctctgacaaa?tgcttgtatt
1441?gccttaagtc?aaagatggat?tactgccaaa?gaagatgatt?taaattcatt?caatgccaca
1501?gacctgaagg?atctctcttc?tcatcaattg?aatgagtttt?tagcacagac?gctccagagg
1561?gcacctcttc?cattggggca?cataaagcga?atgcaagagg?tgtacaactt?caatgccatt
1621?aacaattctg?aaatacgatt?cagatggctg?cggctctgca?ttcaatccaa?gtgggaggac
1681?gcaattcctt?tggcgctaaa?gatggcaact?gaacaaggaa?gaatgaagtt?tacccggccc
1741?ttattcaagg?atcttgctgc?ctttgacaaa?tcccatgatc?aagctgtccg?aacctaccaa
1801?gagcacaaag?caagcatgca?tcccgtgact?gcaatgctgg?tggggaaaga?cttaaaagtg
1861?gattaaagac?ctgcgtattg?atgattttag?agatttctct?tttttaaatg
<210>8
<211>1154
< 213>people
<400>8
BC022313
1 aatcaagaag?ttggcgtgca?gctgggagag?ctagactaag?ttggtcatga?tgcagaagct
61 actcaaatgc?agtcggcttg?tcctggctct?tgccctcatc?ctggttctgg?aatcctcagt
121 tcaaggtaag?actcaggagt?cttgttcccc?agccatcttc?tctgtaagcc?ctgtggtcca
181 tgcaagtcat?tatattcatt?ttaaggcata?gaatgtataa?tattgtgaga?aaggaggcaa
241 agaagaagga?tttggggtcg?ctgaaccctt?taatatgagt?tctgttaagt?ttggtaccaa
301 gaaaaattaa?actctgtggc?gtgtgcagtc?ttgtaaactc?ttacaatgat?tgaaatgtgc
361 tattttggga?tgaaaatgtg?aggtttataa?attttaaaag?ctcaaaaaag?gaatctagaa
421 aatgactcct?gtgcctgttg?catggaggag?atggcacctt?tgactgttgg?ggggtgtctg
481 cctaccccta?agtgtctaca?tcagccccaa?gttttagtgc?gctgtgacgg?tgtcattgtt
541 attttaacac?tgggagacgt?tatattccaa?ttggggtgaa?tctgactgtg?tgtattttct
601 tttctttttt?tttttttaaa?gataaacttg?gttcttactg?aaaactcaat?tatggttaga
661 catagttcat?gtaaaacctc?tcagatttta?aagagaaggc?caaataattt?ggtatttgtg
721 ctcttgctca?gagaagcatc?atattcggaa?atatcttcct?aggtttatct?accatttagt
781 gttgtttagt?cagactgaaa?caacttaaaa?cctgtaatga?ctaagacaat?gaaaatgata
841 ggcttgtaag?aaaaatacaa?tttgttattc?tttggcaaat?aaggaatcat?gtctaaataa
901 gacggaggtc?atggcttgat?agagagatgg?ctgaacctat?agtagaaaaa?cactaggttc
961 cgccaaatgg?taagggaaat?gttgagtcac?aatgacacac?atgtcctaga?tttgtttcgt
121 caaagcgact?tttggttgtc?atgatcttac?ttccggtgga?ggttgcagtg?agctgagatc
1081 atgccattgc?actccaggct?aggcaacaga?gtgcaactcc?gtcccaaaaa?aaaaaaaaaa
1141 aaaaaaaaaa?aaaa
<210>9
<211>976
< 213>people
<400>9
NM?000904
1?cccggaacct?ggcgcaactc?ctagagcggt?ccttggggag?acgcgggtcc?cagtcctgcg
61?gctcctactg?gggagtgcgc?tggtcggaag?attgctggac?tcgctgaaga?gagactacgc
121?aggaaagccc?cagccaccca?tcaaatcaga?gagaaggaat?ccaccttctt?acgctatggc
181?aggtaagaaa?gtactcattg?tctatgcaca?ccaggaaccc?aagtctttca?acggatcctt
241?gaagaatgtg?gctgtagatg?aactgagcag?gcagggctgc?accgtcacag?tgtctgattt
301?gtatgccatg?aactttgagc?cgagggccac?agacaaagat?atcactggta?ctctttctaa
361?tcctgaggtt?ttcaattatg?gagtggaaac?ccacgaagcc?tacaagcaaa?ggtctctggc
421?tagcgacatc?actgatgagc?agaaaaaggt?tcgggaggct?gacctagtga?tatttcagtt
481?cccgctgtac?tggttcagcg?tgccggccat?cctgaagggc?tggatggata?gggtgctgtg
541?ccagggcttt?gcctttgaca?tcccaggatt?ctacgattcc?ggtttgctcc?agggtaaact
601?agcgctcctt?tccgtaacca?cgggaggcac?ggccgagatg?tacacgaaga?caggagtcaa
661?tggagattct?cgatacttcc?tgtggccact?ccagcatggc?acattacact?tctgtggatt
721?taaagtcctt?gcccctcaga?tcagctttgc?tcctgaaatt?gcatccgaag?aagaaagaaa
781?ggggatggtg?gctgcgtggt?cccagaggct?gcagaccatc?tggaaggaag?agcccatccc
841?ctgcacagcc?cactggcact?tcgggcaata?actctgtggc?acgtgggcat?cacgtaagca
901?gcacactagg?aggcccaggc?gcaggcaaag?agaagatggt?gctgtcatga?aataaaatta
961?caacatagct?acctgg
<210>10
<211>2387
< 213>people
<400>10
NM_004994
1?agacacctct?gccctcacca?tgagcctctg?gcagcccctg?gtcctggtgc?tcctggtgct
61?gggctgctgc?tttgctgccc?ccagacagcg?ccagtccacc?cttgtgctct?tccctggaga
121?cctgagaacc?aatctcaccg?acaggcagct?ggcagaggaa?tacctgtacc?gctatggtta
181?cactcgggtg?gcagagatgc?gtggagagtc?gaaatctctg?gggcctgcgc?tgctgcttct
241?ccagaagcaa?ctgtccctgc?ccgagaccgg?tgagctggat?agcgccacgc?tgaaggccat
301?gcgaacccca?cggtgcgggg?tcccagacct?gggcagattc?caaacctttg?agggcgacct
361?caagtggcac?caccacaaca?tcacctattg?gatccaaaac?tactcggaag?acttgccgcg
421?ggcggtgatt?gacgacgcct?ttgcccgcgc?cttcgcactg?tggagcgcgg?tgacgccgct
481?caccttcact?cgcgtgtaca?gccgggacgc?agacatcgtc?atccagtttg?gtgtcgcgga
541?gcacggagac?gggtatccct?tcgacgggaa?ggacgggctc?ctggcacacg?cctttcctcc
601?tggccccggc?attcagggag?acgcccattt?cgacgatgac?gagttgtggt?ccctgggcaa
661?gggcgtcgtg?gttccaactc?ggtttggaaa?cgcagatggc?gcggcctgcc?acttcccctt
721?catcttcgag?ggccgctcct?actctgcctg?caccaccgac?ggtcgctccg?acggcttgcc
781?ctggtgcagt?accacggcca?actacgacac?cgacgaccgg?tttggcttct?gccccagcga
841?gagactctac?acccaggacg?gcaatgctga?tgggaaaccc?tgccagtttc?cattcatctt
901?ccaaggccaa?tcctactccg?cctgcaccac?ggacggtcgc?tccgacggct?accgctggtg
961?cgccaccacc?gccaactacg?accgggacaa?gctcttcggc?ttctgcccga?cccgagctga
1021?ctcgacggtg?atggggggca?actcggcggg?ggagctgtgc?gtcttcccct?tcactttcct
1081?gggtaaggag?tactcgacct?gtaccagcga?gggccgcgga?gatgggcgcc?tctggtgcgc
1141?taccacctcg?aactttgaca?gcgacaagaa?gtggggcttc?tgcccggacc?aaggatacag
1201?tttgttcctc?gtggcggcgc?atgagttcgg?ccacgcgctg?ggcttagatc?attcctcagt
1261?gccggaggcg?ctcatgtacc?ctatgtaccg?cttcactgag?gggcccccct?tgcataagga
1321?cgacgtgaat?ggcatccggc?acctctatgg?tcctcgccct?gaacctgagc?cacggcctcc
1381?aaccaccacc?acaccgcagc?ccacggctcc?cccgacggtc?tgccccaccg?gaccccccac
1441?tgtccacccc?tcagagcgcc?ccacagctgg?ccccacaggt?cccccctcag?ctggccccac
1501?aggtcccccc?actgctggcc?cttctacggc?cactactgtg?cctttgagtc?cggtggacga
1561?tgcctgcaac?gtgaacatct?tcgacgccat?cgcggagatt?gggaaccagc?tgtatttgtt
1621?caaggatggg?aagtactggc?gattctctga?gggcaggggg?agccggccgc?agggcccctt
1681?ccttatcgcc?gacaagtggc?ccgcgctgcc?ccgcaagctg?gactcggtct?ttgaggagcg
1741?gctctccaag?aagcttttct?tcttctctgg?gcgccaggtg?tgggtgtaca?caggcgcgtc
1801?ggtgctgggc?ccgaggcgtc?tggacaagct?gggcctggga?gccgacgtgg?cccaggtgac
1861?cggggccctc?cggagtggca?gggggaagat?gctgctgttc?agcgggcggc?gcctctggag
1921?gttcgacgtg?aaggcgcaga?tggtggatcc?ccggagcgcc?agcgaggtgg?accggatgtt
1981?ccccggggtg?cctttggaca?cgcacgacgt?cttccagtac?cgagagaaag?cctatttctg
2041?ccaggaccgc?ttctactggc?gcgtgagttc?ccggagtgag?ttgaaccagg?tggaccaagt
2101?gggctacgtg?acctatgaca?tcctgcagtg?ccctgaggac?tagggctccc?gtcctgcttt
2161?ggcagtgcca?tgtaaatccc?cactgggacc?aaccctgggg?aaggagccag?tttgccggat
2221?acaaactggt?attctgttct?ggaggaaagg?gaggagtgga?ggtgggctgg?gccctctctt
2281?ctcacctttg?ttttttgttg?gagtgtttct?aataaacttg?gattctctaa?cctttaaaaa
2341?aaaaaaaaaa?aaaaaaaaaa?aaaaaaaaaa?aaaaaaaaaa?aaaaaaa

Claims (1)

1. a combination is used to screen the gene of ductal adenocarcinoma of pancreas; It is characterized in that; Said gene is made up of L48784, X83300, AI620827, AI494291, NM_020970, BF433657, J02959, BC022313, NM_000904, NM_004994, and its gene order is respectively SEQ ID NO:1～SEQ ID NO:10.
CN2006100526794A 2006-07-28 2006-07-28 Gene composition for screening pancreatic catheter gland cancer and its application Expired - Fee Related CN1904072B (en)

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Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004031411A2 (en) * 2002-09-30 2004-04-15 Oncotherapy Science, Inc. Genes and polypeptides relating to human pancreatic cancers

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004031411A2 (en) * 2002-09-30 2004-04-15 Oncotherapy Science, Inc. Genes and polypeptides relating to human pancreatic cancers

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Craig D. Logsdon, et al..Molecular Profiling of Pancreatic Adenocarcinoma and Chronic Pancreatitis Identifies Multiple Genes Differentially Regulated in Pancreatic Cancer.Cancer Research63.2003,632649-2657. *
Haiyong Han, et al..Identification of Differentially Expressed Genes in Pancreatic Cancer Cells Using cDNA Microarray.Cancer Research62.2002,622890-2896. *

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