CN1899595A - Preparing process for Chinese medicine for treating chronic simple largngitis and laryngopharyngeal parestesia and its use - Google Patents
Preparing process for Chinese medicine for treating chronic simple largngitis and laryngopharyngeal parestesia and its use Download PDFInfo
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- CN1899595A CN1899595A CN 200610043109 CN200610043109A CN1899595A CN 1899595 A CN1899595 A CN 1899595A CN 200610043109 CN200610043109 CN 200610043109 CN 200610043109 A CN200610043109 A CN 200610043109A CN 1899595 A CN1899595 A CN 1899595A
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Abstract
The present invention is the preparation process and application of Chinese medicine for treating chronic simple laryngitis and laryngopharyngeal paresthesia. The preparation process is stable and feasible and has raised material utilizing rate and high effective component extracting rate. The Chinese medicine has raised hesperidin content and possesses the functions of eliminating sputum, promoting the secretion of tracheal glands and inhibiting the proliferation of granulation tissue.
Description
Technical field:
The present invention relates to preparation technology of a kind of Chinese medicine preparation and uses thereof, particularly relate to preparation technology of a kind of pure Chinese medicinal preparation for the treatment of chronic simple laryngitis, laryngopharyngeal paresthesia and uses thereof.
Background technology:
Throat disease is common clinical, frequently-occurring disease, sickness rate has growth trend in China in recent years, the ill crowd of China's throat disease constantly increases at present, according to relevant clinical medicine report, suffer from various acute and chronic laryngopharyngeal diseases patient and be about 12% of domestic total number of persons, the height of sickness rate will cause medical circle and compatriots' attention.
Pharyngeal discomfort, laryngopharyngeal paresthesia, pachyderma circumscripta are because long-term sounding is improper, sing absurd, the powerful heavy burden or phlegm resistance, throat commonly encountered diseases due to the stagnation of QI due to depression of the liver though the domestic medicine that is used for the treatment of laryngopharyngitis has last ten kinds more than at present, mostly is a kind of medicine Zhiduo County and plants sick, curative effect is not very good, especially treat chronic hoarseness, pharyngeal different sense disease (traditional Chinese medical science claims globus hystericus), the medicine of diseases such as pachyderma circumscripta is almost very little.Clinical common method is a western medical treatment, take sound to stop or implement operation to cooperate antibiosis control disease usually, but negative effect is big, causes suffering to the patient.
CN1544076A discloses a kind of " Chinese medicine of treatment laryngopharyngeal paresthesia ", the preparation technology of its tablet, capsule loses more in the concentration process of raw material, spissated extractum density is unfavorable for mixing, be difficult for dry, take a lot of work, time-consuming, and utilization ratio of raw materials, extraction ratio of effective constituents are not high.In commercial production, how to guarantee that the every batch of finished product total amount produced that feeds intake can keep stable in the amplitude of the permission that national drug is managed, stable by every data of national legal drug inspection and result simultaneously, the favorable reproducibility of experiment is an important techniques key point.Therefore work out that finished product is stable, determined curative effect, the Chinese prescription preparation for the treatment of both the principal and secondary aspects of a disease has important practical significance.
Summary of the invention:
The object of the invention is to provide a kind of preparation technology who treats the Chinese medicine preparation of chronic simple laryngitis, laryngopharyngeal paresthesia.
Another purpose of the present invention is to be provided in the medicine for the treatment of chronic simple laryngitis, laryngopharyngeal paresthesia and uses.
The present invention seeks to be achieved through the following technical solutions:
Get Poria, Fructus Amomi, the Arisaema Cum Bile of recipe quantity 40%-60%, be ground into fine powder, sieve, mixing, standby; With surplus after the pulverizing of Rhizoma Pinelliae Preparatum, Fructus Aurantii Immaturus, Pericarpium Citri Reticulatae Viride, dried tangerine peel, Fructus Amomi Rotundus, Semen Arecae, Cortex Albiziae, Massa Medicata Fermentata, Caulis Perillae, Rhizoma Zingiberis Recens, Periostracum Cicadae, Semen Oroxyli, Cortex Magnoliae Officinalis and Poria, Fructus Amomi, Arisaema Cum Bile three flavor medicines add 8-12 doubly, a 6-10 times decocting boils secondary, each 1-3 hour, collecting decoction, filter, filtrate is concentrated into the clear paste that relative density is 1.15～1.20 (60 ℃), add ethanol and make pure content reach 50-70%, standing over night is got supernatant; Reclaim ethanol, being evaporated to relative density is the thick paste of 1.28～1.32 (60 ℃), adds the fine powder mixing of preparation, and drying is pulverized, and makes fine particle, promptly.
Its process stabilizing of preparation technology of the present invention, feasible, the content of hesperidin of finished product has improved utilization ratio of raw materials, extraction ratio of effective constituents than former technology height; The present invention has phlegm-dispelling functions, can promote the tracheal glands secretion; Inhibited to the chronic inflammatory disease granulation tissue hyperplasia.The finished product treatment chronic simple laryngitis of extraction process by water preparation, total effective rate 73.3% is treated pharyngeal different sense disease, total effective rate 76.7%; The finished product treatment chronic simple laryngitis of aqueous extraction-alcohol precipitation technology preparation, its total effective rate 90.0% is treated pharyngeal different sense disease, its total effective rate 93.3%, thus improved the clinical efficacy for the treatment of chronic simple laryngitis, laryngopharyngeal paresthesia.
The specific embodiment
Following experimental example is used to further specify the present invention.
Experimental example 1 extraction process by water is preferred
We are by analyzing multiple factor, and the design orthogonal test is selected optimum extraction process.The medical material water absorption is investigated: press recipe quantity, beat the powder except that Poria, Fructus Amomi, each 1/2 amount of Arisaema Cum Bile, take by weighing 13 flavors and above-mentioned three surpluses of distinguishing the flavor of such as Fructus Aurantii Immaturus, add 10 times of water gagings to be dipped to the heart, filter, measuring water absorption rate is 210%.
1, the design of orthogonal test is through analyzing contrast to factors such as the related extraction time of extraction process, extraction time, amount of water, and each designs 3 levels, design factor water-glass (table 1).
Table 1 extraction process factor level table
| Factor level | Extraction time (inferior) A | Extraction time (h) B | Amount of water (doubly) C |
| 1 2 3 | 1 2 3 | 1 2 3 | 10(8，8) * 12(10，10) * 14(12，12) * |
Annotate:
*Be respectively amount of water in the bracket the 2nd, 3 time
2, test method is pressed recipe quantity, take by weighing Poria, Fructus Amomi, Arisaema Cum Bile recipe quantity all the other 13 flavor medical material recipe quantities such as 1/20 and Fructus Aurantii Immaturus 1/10, totally 9 parts, according to L
9(3
4) test (table 2) of orthogonal table design, decoct with water, filter, 70 ℃ (0.08Mpa) concentrating under reduced pressure and evaporate to dryness, 80 ℃ (0.08Mpa) drying under reduced pressure is 3 hours, weighs for filtrate.Measure Determination of Hesperidin Content in the dried cream, the results are shown in Table 2.
Chromatographic condition and system suitability test are filler with octadecylsilane chemically bonded silica; Methanol-water (25: 75) is a mobile phase; The detection wavelength is 283nm.Number of theoretical plate calculates by the Hesperidin peak should be not less than 1000.
It is an amount of that the preparation precision of reference substance solution takes by weighing the Hesperidin reference substance, adds methanol and make the solution that every 1ml contains 0.1mg, promptly.
It is an amount of that above-mentioned dried cream is got in the preparation of need testing solution, and porphyrize is got about 0.1g, accurate claims surely, puts in the 25ml measuring bottle, adds methanol 20ml, and supersound process 30 minutes is put coldly, adds methanol to scale, shakes up, promptly.
Accurate respectively reference substance solution and each the 10 μ l of need testing solution of drawing of algoscopy inject chromatograph of liquid, measure, promptly.
Table 2 extraction process orthogonal experiments
| The factor level tested number | Extraction time A (inferior) | Extraction time B (h) | Amount of water C (doubly) | Dried cream amount (g) | Content of hesperidin (%) | Total score |
| 1 2 3 4 5 6 7 8 9 | 1 1 1 2 2 2 3 3 3 | 1 2 3 1 2 3 1 2 3 | 10(8，8) 12(10，10) 14(12，12) 12(10，10) 14(12，12) 10(8，8) 14(12，12) 10(8，8) 12(10，10) | 10.6 12.9 13.5 18.6 26.8 26.1 20.5 25.8 27.1 | 0.46 0.50 0.49 0.61 0.57 0.55 0.57 0.52 0.51 | 60.89 68.22 68.12 87.45 95.62 92.62 86.32 89.23 90.16 |
| K 1 K 2 K 3 R | 197.23 275.69 265.71 78.46 | 234.66 253.07 250.90 18.41 | 242.74 245.83 250.06 7.32 | K＝K 1+K 2+K 3＝738.63 | ||
Annotate: this orthogonal test adopts two evaluation indexes, take all factors into consideration dried cream amount and Hesperidin effect in this product, adopt comprehensive grading to carry out data analysis, dried cream amount of comprehensive grading and content of hesperidin weight coefficient are respectively 0.4 and 0.6, i.e. comprehensive grading=dried cream amount scoring * 0.4+ Hesperidin amount scoring * 0.6.Dried cream amount scoring=dried cream amount/maximum dry cream amount * 100; Hesperidin amount scoring=Hesperidin amount/maximum Hesperidin amount * 100.
3, test result analysis from orthogonal test gained result as can be seen, A factor extreme difference is 78.46, B factor extreme difference is 18.41, C factor extreme difference is 7.32, A＞B＞C, the A factor has the greatest impact to the extraction of this product, and the B factor is bigger to the extraction influence of this product, and the C factor is minimum to the extraction influence of this product.Analyze A factor K from the K value
2(275.69)＞K
3(265.71)＞K
1(197.23), should select A
2B factor K
2(253.07)＞K
3(250.90)＞K
1(234.66), should select B
2C factor K
3(250.06)＞K
2(245.83)＞K
1(242.74), should select C
3From interpretation of result as can be known, the optimum extraction process of this product should be A
2B
2C
3And above-mentioned result of the test carried out statistical analysis, the results are shown in Table 3.
Table 3 The results of analysis of variance
| Soruces of variation | Sum of sguares of deviation from mean | Degree of freedom | Mean square | F | P | Factor affecting |
| Extraction time extraction time amount of water error | 1216.20 67.45 9.02 1.04 | 2 2 2 2 | 608.10 33.73 4.51 0.52 | 1169.42 64.87 8.67 1.00 | ＜0.01 ＜0.05 ＞0.05 | Highly significant is significantly or not |
F
0.05(2，2)＝19.0 F
0.01(2，2)＝99.0
As can be seen from Table 3, extraction time has highly significant influence (P＜0.01) to result of the test in this test; There is significance influence (P＜0.05) extraction time to result of the test; Amount of water is to result of the test influence not significantly (P＞0.05), considers in conjunction with industrial practical situation and energy efficient and the angle that reduces production costs, with C
3Be adjusted into C
1, the technology after determining to optimize is A
2B
2C
1, promptly the extraction process of this product adds 8 times of water gagings for the second time for adding for the first time 10 times of water gagings, decocts secondary, and each 2 hours, collecting decoction filtered, and filtrate (0.08Mpa) is evaporated to the clear paste that relative density is 1.12-1.18 (50 ℃ of survey) for 70 ℃.
Experimental example 2 alcohol precipitation processes are preferred
This product also extracts invalid components such as starch, phlegmatic temperament, protein, resin, tannin when decocting with water the effective ingredient of extraction side's Chinese medicine, in order to improve drug level, reduce taking dose, guarantee curative effect of medication, we carry out purification process to water decoction extract, to remove invalid components such as destarching, phlegmatic temperament, protein, resin, tannin.According to the physicochemical property of each flavour of a drug in this product prescription, in conjunction with actual production conditions, we carry out purification by alcohol deposition method to extract, and concentration of alcohol is investigated during now to precipitate with ethanol.
Press recipe quantity, take by weighing Poria, Fructus Amomi, 1/10 of all the other 13 flavor medical material recipe quantities of 1/20 and the Fructus Aurantii Immaturus etc. of Arisaema Cum Bile recipe quantity, totally 9 parts, with every part of medical material mix homogeneously, decoct with water secondary respectively, add 10 times of water gagings for the first time, add for the second time 8 times of water gagings, each 2 hours, collecting decoction filtered, filtrate is concentrated into the clear paste that relative density is 1.12-1.18 (50 ℃ of survey), per three parts add ethanol respectively to 50%, 65% and 80%, stir evenly, left standstill 24 hours, filter, 70 ℃ (0.08Mpa) concentrating under reduced pressure and evaporate to dryness, 80 ℃ (0.08Mpa) drying under reduced pressure is 3 hours, weighs for filtrate.Measure Determination of Hesperidin Content in the dried cream, the results are shown in Table 4.
Table 4 different concentration ethanol precipitate with ethanol experimental result
| Sequence number | 50% ethanol | 65% ethanol | 80% ethanol | |||
| Dried cream amount (g) | Determination of Hesperidin Content (%) | Dried cream amount (g) | Determination of Hesperidin Content (%) | Dried cream amount (g) | Determination of Hesperidin Content (%) | |
| 123 is average | 19.3 19.6 19.7 19.5 | 0.80 0.79 0.78 0.79 | 16.2 16.0 15.9 16.0 | 0.96 0.96 0.97 0.96 | 15.6 15.8 15.5 15.6 | 0.98 0.97 0.99 0.98 |
Show that by table 4 result of the test dried cream amount of 65% and 80% ethanol and Determination of Hesperidin Content are more or less the same during precipitate with ethanol, but obviously reduce that content of hesperidin obviously improves than the dried cream amount of 50% ethanol.No matter be 50%, 65% and 80% ethanol in addition, Hesperidin does not substantially all have loss behind the precipitate with ethanol.Energy resource consumption when considering commercial production and saving cost, concentration of alcohol is 65% when determining precipitate with ethanol, leaves standstill 24 hours, filters filtrate recycling ethanol.
Experimental example 3 aqueous extraction-alcohol precipitation technologies and extraction process by water are preferred
The parameter of determining according to above-mentioned extraction process by water and aqueous extraction-alcohol precipitation technology feeds intake by 10 times of amounts of prescription and to carry out middle trial production, shaped granule is investigated, relatively two kinds of technology similarities and differences.
1. three batches of pilot product result of the tests relatively
Three batches of pilot product result of the tests see Table 5.
Three batches of pilot product result of the tests of table 5 different process
| Lot number | A01 | A02 | A03 | B01 | B02 | B03 |
| Medicinal material is beaten powder inventory (kg) fine powder amount (kg) flour extraction (%) medicinal material extract inventory (Kg) and is extracted medicinal extract amount (Kg) the alcohol medicinal extract amount (Kg) that settles out and extract dried cream amount (Kg) granulation amount (Kg) | 2.475 2.36 95.4 21.698 17.32 2.73 5.01 | 2.475 2.38 96.2 21.698 19.75 2.92 5.04 | 2.475 2.37 95.8 21.698 18.36 2.67 4.88 | 2.475 2.37 95.8 21.698 5.17 1.78 4.11 | 2.475 2.39 96.6 21.698 5.21 1.75 4.07 | 2.475 2.38 96.2 21.698 5.12 1.72 3.97 |
* A is an extraction process by water, and B is an aqueous extraction-alcohol precipitation technology
As can be seen from the above table, extraction process by water extracts the extractum amount and dried cream amount is all more, differing greatly between batch, and the technology instability, dried cream amount is many, does not reach the purpose that reduces dose.Aqueous extraction-alcohol precipitation technology has effectively been removed dis-medicinal part, has kept effective ingredient, and parameter stability between the technology, dose meet national drug consumption regulation.
2. granule draws moist investigation
Take by weighing each about 100g of shaped granule that this product aqueous extraction-alcohol precipitation technology and extraction process by water are made respectively, be positioned over (35 ℃ of temperature, relative humidity 70%) under the natural conditions, surveyed its water absorption rate once, and the results are shown in Table 6 in per 24 hours.
Table 6 different process draws moist test
| Batch | Water absorption rate (%) | |||||
| 0h | 24h | 48h | 72h | 96h | 120h | |
| A01 A02 A03 | 0 0 0 | 2.72 2.51 2.72 | 3.76 3.72 3.82 | 4.83 4.86 4.89 | 5.47 5.76 5.71 | 5.68 5.62 5.61 |
| B01 B02 B03 | 0 0 0 | 3.10 3.12 2.96 | 3.62 3.53 3.66 | 3.64 3.61 3.61 | 3.87 3.80 3.62 | 3.89 3.91 3.78 |
* A is an extraction process by water, and B is an aqueous extraction-alcohol precipitation technology
By last table result as can be seen, its average water absorption rate of the finished product of aqueous extraction-alcohol precipitation technology preparation changes little, and stability of sample is better.
3. mobility of particle is investigated
The flowability of the shaped granule of this product aqueous extraction-alcohol precipitation technology and extraction process by water being made with self-control " funnel " method is checked, the results are shown in Table 7,8.
Table 7 different process fluidity testing
| Batch | The 1st minute | The 2nd minute | The 3rd minute |
| A01 A02 A03 | 61.5 63.5 62.7 | 62.4 64.8 62.9 | 62.8 64.4 63.1 |
| B01 B02 B03 | 68.5 68.7 67.6 | 67.9 69.4 68.1 | 68.3 69.8 68.3 |
* A is an extraction process by water, and B is an aqueous extraction-alcohol precipitation technology
Table 8 different process content measurement result angle of repose
| Batch | Content angle of repose | |||
| 1 | 2 | 3 | On average | |
| A01 A02 A03 | 43°48’ 42°21’ 43°40’ | 43°40’ 41°58’ 42°58’ | 43°34’ 42°53’ 42°41’ | 43°41’ 42°31’ 43°6’ |
| B01 B02 B03 | 36°40’ 38°24’ 35°29’ | 35°57’ 37°50’ 36°8’ | 36°6’ 37°43’ 35°52’ | 36°21’ 37°59’ 35°50’ |
* A is an extraction process by water, and B is an aqueous extraction-alcohol precipitation technology
By last table result as can be seen, the finished particle of aqueous extraction-alcohol precipitation technology preparation is better mobile, is easy to particulate filling and packing, is fit to large-scale production.
4. product quality analysis
The shaped granule that aqueous extraction-alcohol precipitation technology and extraction process by water are made detects according to the quality standard of working out, and investigates the influence of different process to product quality, thereby determines rational production technology.Experimental result sees Table 9.
Three batches of different process product quality analysis of table 9
| Lot number | A01 | A02 | A03 | B01 | B02 | B03 |
| The TLC of character physicochemical identification Neosynephrine differentiates that the TLC of Hesperidin differentiates assay (mg/g) | Negative reaction up to specification detects 2.17 | Positive reaction up to specification detects 2.15 | Positive reaction up to specification detects 2.12 | Positive reaction up to specification detects 3.82 | Positive reaction up to specification detects 3.87 | Positive reaction up to specification detects 3.84 |
* A is an extraction process by water, and B is an aqueous extraction-alcohol precipitation technology
By the result of last table as can be seen, the non-medicinal component that extraction process by water proposes is more, has disturbed the detection to drug standard, can not accurately control drug quality, the technology instability; The sample of aqueous extraction-alcohol precipitation technology differentiates that Xiang Junneng detects, and content of hesperidin is higher, and the diversity of content is little, shows that the technology stability behind the precipitate with ethanol is good.
5. product stability analysis
Capsule to aqueous extraction-alcohol precipitation technology and extraction process by water are made carries out accelerated test, in temperature (40 ± 2) ℃, placed six months under the condition of relative humidity (70 ± 5) %, sampling in every month detects once, detects by stable high spot reviews project, the results are shown in Table 10,11.
Table 10 extraction process by water is made finished product stability and is detected
| Lot number and check | Character | Microscopical identification | The TLC of Neosynephrine differentiates | The TLC of Hesperidin differentiates | Moisture (%) | Assay (mg/g) | Health examination | |
| A01 | 1 month 2 months 3 months 4 months 5 months 6 months | No change no change no change no change no change no change | Detect | Do not detect | Detect | 6.7 6.9 6.5 6.7 6.4 6.2 | 2.17 2.16 2.14 2.12 2.14 2.11 | Do not detect |
| A02 | 1 month | No change | Detect | Detect | Detect | 6.5 | 2.15 | Do not detect |
| 2 months 3 months 4 months 5 months 6 months | No change no change no change no change no change | Detect | Do not detect | Detect | 6.7 6.2 6.7 6.9 6.8 | 2.15 2.16 2.12 2.10 2.13 | Do not detect | |
| A03 | 1 month 2 months 3 months 4 months 5 months 6 months | No change no change no change no change no change no change | Detect | Do not detect | Detect | 6.5 6.7 6.4 6.7 6.8 7.0 | 2.16 2.11 2.14 2.12 2.11 2.10 | Do not detect |
Table 11 aqueous extraction-alcohol precipitation technology is made finished product stability and is detected
| Lot number and check | Character | Microscopical identification | The TLC of Neosynephrine differentiates | The TLC of Hesperidin differentiates | Moisture (%) | Assay (mg/g) | Health examination | |
| B01 | 1 month 2 months 3 months 4 months 5 months 6 months | No change no change no change no change no change no change | Detect | Detect | Detect | 4.8 4.7 4.9 5.1 5.0 5.3 | 3.84 3.85 3.83 3.81 3.84 3.82 | Do not detect |
| B02 | 1 month 2 months 3 months 4 months 5 months 6 months | No change no change no change no change no change no change | Detect | Detect | Detect | 5.1 5.2 5.0 4.7 5.3 5.4 | 3.87 3.82 3.87 3.82 3.81 3.82 | Do not detect |
| B03 | 1 month 2 months 3 months 4 months 5 months 6 months | No change no change no change no change no change no change | Detect | Detect | Detect | 5.1 5.3 4.9 5.3 5.4 5.8 | 3.85 3.82 3.79 3.80 3.78 3.81 | Do not detect |
* A is an extraction process by water, and B is an aqueous extraction-alcohol precipitation technology
By table 10,11 as can be seen, the sample of extraction process by water has disturbed the detection to drug standard because the non-medicinal component that proposes is more, and the content difference opposite sex is big and hygroscopicity is big; The sample of aqueous extraction-alcohol precipitation technology detects according to the quality standard of working out, and differentiates that Xiang Junneng detects, and content of hesperidin is higher, and the diversity of content is little, shows that the technology stability behind the precipitate with ethanol is good.
Experimental example 4 test (the phenol red excretion of trachea section is mensuration) of reducing phlegm
Be subjected to the reagent thing:
Title: Jinsangliyan capsule
Character: brown granular and powder; Feeble QI, mildly bitter flavor.
The unit of providing: the Forest of Steles in Xi'an pharmaceutical factory of traditional Chinese medicine
Lot number: water extract-alcohol precipitation extract capsule 950201 water extract capsules 950202
Content: water extract-alcohol precipitation extract capsule 950201 0.4g/ grains
Water extract capsule 950202 0.4g/ grains
Solvent and compound method: with the preceding suspension that is made into desired concn with distilled water
Contrast medicine and main agents: prednisone sheet [the accurate word (1993) of medicine is defended No. 000532 in Shan], Xi'an pharmaceutical factory produces, lot number 9501027; Voltoide [the accurate word (1981) of medicine is defended No. 001150 in Tianjin], Lik-Sang pharmaceutical factory in Tianjin produces, lot number 945604; Phenol red, reagent three factories in Shanghai produce, lot number 940112
Experimental apparatus 752C type ultraviolet-uisible spectrophotometer, Shanghai the 3rd analytical tool factory produces;
The JA1003 electronic balance, Shanghai Precision Scientific Apparatus Co., Ltd produces.
Laboratory animal ICR strain mice, the 18-22g certification of fitness: the moving card of Shan doctor word 08-004 number; SD
The strain rat, 200～250g certification of fitness: the moving card of Shan doctor word 08-005 number, all
Provide by Xian Medical Univ's Experimental Animal Center.Sex: ♂ ♀ half and half
20～26 ℃ of relative humiditys 42～76% of experiment room temperature.
Get 80 of mices, male and female half and half are divided into 8 groups at random, 10 every group.First group is the blank group, gives the isometric(al) normal saline; Second group of positive matched group given voltoide 1g/kg; Third and fourth, five groups be Jinsangliyan water extract Capsules group, the 6th, seven, eight group is Jinsangliyan water extract-alcohol precipitation extract capsule group; Two kinds of different extract capsules are respectively with 0.2072g, 0.4144g, 0.8288g/kg (crude drug) ig administration.2 times/day, for three days on end.Behind the last administration 30min, the phenol red 0.1ml/10g of ip5% puts to death mice behind 30min, peel off the trachea surrounding tissue, cut one section trachea to the trachea crotch, put into the 2ml normal saline from thyroid cartilage, add 2 of 1mol/L sodium hydroxide, the 546nm colorimetric is calculated phenol red content.Check through t.The results are shown in Table 12.
Two kinds of Jinsangliyan capsules of table 12 are to the influence of the phenol red excretion amount of mice trachea section (X ± S)
| Group | Dosage (g/kg) | Number of animals (only) | Phenol red excretion amount (g/ml) |
| In the blank group voltoide water extraction relieve sore throat small amount of water Tilly pharynx water gaging Tilly swallow a large amount of water extract-alcohol precipitation relieve sore throats in a small amount in the water extract-alcohol precipitation relieve sore throats water gaging to carry the alcohol precipitation relieve sore throat a large amount of | 1.0 0.2072 0.4144 0.8288 0.2072 0.4144 0.8288 | 10 10 10 10 10 10 10 10 | 1.08±0.32 2.19±0.91** 1.12±0.55 1.30±0.53 1.74±0.93* 1.62±0.31* 1.82±0.40* 1.96±0.51** |
Compare * P＜0.05 with the blank group; * P＜0.01
By table 12 as seen, compare with the blank group, Jinsangliyan water extract-alcohol precipitation extract capsule and water extract capsule all can make the phenol red excretion amount of mice trachea increase, the heavy dose of group of water extract capsule has significant difference (P＜0.05), the water extract-alcohol precipitation extract capsule is then little, in, heavy dose all has significant difference (P＜0.05 P＜0.01); The result shows that Jinsangliyan water extract-alcohol precipitation extract capsule and water extract capsule all can promote the tracheal glands secretion, and mice is had phlegm-dispelling functions; And water extract-alcohol precipitation extract capsule drug effect is better than the water extract capsule.
Experimental example 5 antiinflammatories tests (agar hypodermic injection)
Be subjected to the reagent thing:
Title: Jinsangliyan capsule
Character: brown granular and powder; Feeble QI, mildly bitter flavor.
The unit of providing: the Forest of Steles in Xi'an pharmaceutical factory of traditional Chinese medicine
Lot number: water extract-alcohol precipitation extract capsule 950201 water extract capsules 950202
Content: water extract-alcohol precipitation extract capsule 950201 0.4g/ grains
Water extract capsule 950202 0.4g/ grains
Solvent and compound method: with the preceding suspension that is made into desired concn with distilled water
Contrast medicine and main agents: prednisone sheet [the accurate word (1993) of medicine is defended No. 000532 in Shan], Xi'an pharmaceutical factory produces, lot number 9501027; Voltoide [the accurate word (1981) of medicine is defended No. 001150 in Tianjin], Lik-Sang pharmaceutical factory in Tianjin produces, lot number 945604; Phenol red, reagent three factories in Shanghai produce, lot number 940112
Experimental apparatus 752C type ultraviolet-uisible spectrophotometer, Shanghai the 3rd analytical tool factory produces;
The JA1003 electronic balance, Shanghai Precision Scientific Apparatus Co., Ltd produces.
Laboratory animal ICR strain mice, the 18-22g certification of fitness: the moving card of Shan doctor word 08-004 number; SD
The strain rat, 200～250g certification of fitness: the moving card of Shan doctor word 08-005 number, all
Provide by Xian Medical Univ's Experimental Animal Center.Sex: ♂ ♀ half and half
20～26 ℃ of relative humiditys 42～76% of experiment room temperature.
Get 80 of rats, male and female half and half are divided into 8 groups at random, 10 every group.First group is the blank group, gives the isometric(al) normal saline; Second group of positive matched group given prednisone sheet 8mg/kg; Third and fourth, five groups be Jinsangliyan water extract Capsules group, the 6th, seven, eight group is Jinsangliyan water extract-alcohol precipitation extract capsule group; Under the shallow fiber crops of ether, the cropping sterilization is in rat back center line subcutaneous injection 2% agar solution 2ml.Two kinds of different extract capsule groups are respectively with 0.2072g, 0.4144g, 0.8288g/kg (crude drug) gastric infusion, and 2 times/day, 14d continuously.D15 puts to death rat, carefully peels off and takes out the granuloma agar block, removes agar, claims the granulation tissue weight in wet base.The result represents with the contained granulation tissue weight of every 100g body weight.Check through t.See Table 13.
Two kinds of Jinsangliyan capsules of table 13 are to the influence of rat chronic proliferative inflammation (X ± S)
| Group | Dosage (g/kg) | Number of animals (only) | Granulation tissue weight (mg/100g body weight) |
| In the blank group prednisone water extraction relieve sore throat small amount of water Tilly pharynx water gaging Tilly swallow a large amount of water extract-alcohol precipitation relieve sore throats in a small amount in the water extract-alcohol precipitation relieve sore throats water gaging to carry the alcohol precipitation relieve sore throat a large amount of | 0.008 0.2072 0.4144 0.8288 0.2072 0.4144 0.8288 | 10 10 10 10 10 10 10 10 | 48.2±1.72 26.4±0.80** 47.5±1.44 35.7±0.84 30.2±0.91* 40.2±1.42 31.3±1.24* 24.6±1.08** |
Compare * P＜0.05 with the blank group; * P＜0.01
By table 13 as seen, compare with the blank group, Jinsangliyan water extract capsule and water extract-alcohol precipitation extract capsule all have the rat granulation tissue hyperplasia effect that agar stimulates that suppresses, the heavy dose of group of water extract capsule has significant difference (P＜0.05), the water extract-alcohol precipitation extract capsule then in, heavy dose all has significant difference (P＜0.05 P＜0.01); The result shows that the Jinsangliyan capsule has inhibitory action to the rat chronic proliferative inflammation, and shows that water extract-alcohol precipitation extract capsule drug effect is better than the water extract capsule.
Experimental example 6 Jinsangliyan capsule for treating chronic simple laryngitises, the clinical research of pharyngeal different sense disease
One, clinical trial
Test method
1, group technology: this test intended adopts random table method grouping contrast.
2, observe routine number: A and organize 60 examples, wherein single sick each 30 example of planting; B organizes 60 examples, wherein single sick each 30 example of planting.
3, trial drug and using method
A group: aqueous extraction-alcohol precipitation technology is produced the Jinsangliyan capsule, specification: every dress 0.4g, every contains primary dose 2.417g, usage and dosage: oral, one time 3,2 times on the one.
B group: extraction process by water is produced the Jinsangliyan capsule, specification: every dress 0.4g, every contains primary dose 1.2085g, usage and dosage: oral, one time 6,2 times on the one.
Two groups day takes the crude drug amount and is 14.5g, has comparability.
4, observe the course of treatment
This test (7 days) weekly is a course of treatment, and long run test is observed 3 courses of treatment (21 days).
5, the test case adopts clinic case, should grasp patient's compliance, strict control variable factor.
Curative effect determinate standard:
Chronic simple laryngitis (chronic hoarseness)
1, recovery from illness: pronunciation recovers normal, throat's transference cure, and throat checks normal, therapeutic index 〉=90% is followed up a case by regular visits to and is not had recurrence in 3 months.
2, produce effects: hoarseness obviously improves, and throat's symptom is most of to disappear, and throat checks obviously and improves therapeutic index 〉=70%.
3, effective: hoarseness has improvement, throat's sx, and throat checks improvement, therapeutic index 〉=30%.
4, invalid: sings and symptoms no change, therapeutic index＜30%.
The different sense disease of throat (globus hystericus)
Recovery from illness: foreign body in pharynx sense complete obiteration, corresponding transference cure, therapeutic index 〉=90%;
Produce effects: the foreign body in pharynx sense obviously alleviates, and corresponding symptom obviously alleviates, therapeutic index 〉=70%;
Effectively: the foreign body in pharynx sense obviously alleviates, and corresponding symptom has improvement, therapeutic index 〉=30%;
Invalid: the foreign body in pharynx sense does not have obviously and alleviates, and corresponding symptom does not have improvement, therapeutic index＜30%.
The statistical disposition of testing data
1, enumeration data should adopt X 2 test.
2, measurement data should adopt the t check, and wherein two sample means relatively with the t check, are relatively used paired t-test before and after self.
3, ranked data should adopt the Ridit check.
Two, case data
Sex distributes
Table 1a 60 routine chronic simple laryngitis sexes distribute
| Group | Total routine number | The man | The woman |
| A group B group | 30 30 | 12 14 | 18 16 |
Two groups of sexes distribute and contrast, through X 2 test x
2=0.068, P＞0.05, difference does not have the significance meaning, and comparability is arranged.
The pharyngeal different sense disease sex of table 1b 60 examples distributes
| Group | Total routine number | The man | The woman |
| A group B group | 30 30 | 13 15 | 17 15 |
Two groups of sexes distribute and contrast, through X 2 test x
2=0.067, P＞0.05, difference does not have the significance meaning, and comparability is arranged.
Age distribution
Table 2a 60 routine chronic simple laryngitis age distribution
| Group | Total routine number | 18-30 year | 31-40 year | 41-50 year | 51-65 year |
| A group B group | 30 30 | 2 3 | 8 7 | 15 15 | 5 5 |
A group minimal ages 18 years old, maximum 62 years old age, 41.2 years old mean age; B group minimal ages 19 years old, maximum 57 years old age, 34.2 years old mean age.Two groups of age distribution ratios, through X 2 test, x
2=0.267, P＞0.05, difference does not have the significance meaning, and comparability is arranged.
The age distribution of the pharyngeal different sense disease of table 2b 30 examples
| Group | Total routine number | 18-30 year | 31-40 year | 41-50 year | 51-65 year |
| A group B group | 30 30 | 2 1 | 10 8 | 9 11 | 9 10 |
A group minimal ages 21 years old, maximum 63 years old age, 41.5 years old mean age; B group minimal ages 20 years old, maximum 58 years old age, 37.5 years old mean age.Two groups of age distribution ratios, through X 2 test, x
2=0.808, P＞0.05, difference does not have the significance meaning, and comparability is arranged.
The course of disease distributes
The table 3a 60 routine chronic simple laryngitis courses of disease distribute
| Group | Total routine number | 2 months - | Half a year - | 1 year - | 2 years - | 3 years - |
| A group B group | 30 30 | 2 3 | 3 7 | 8 7 | 8 8 | 9 5 |
The A group course of disease is the shortest 2.1 months, the longest 4.3 years, average course of disease 2.4 years; The B group course of disease is the shortest 3.2 months, the longest 3.6 years, average course of disease 1.8 years.Two groups of courses of disease distribute and contrast, through X 2 test, and X
2=3.01, P＞0.05, difference does not have the significance meaning, and comparability is arranged.
The pharyngeal different sense disease course of disease of table 3b 60 examples distributes
| Group | Total routine number | 2 months - | Half a year - | 1 year - | 2 years - | 3 years - |
| A group B group | 30 30 | 6 5 | 9 7 | 7 8 | 3 6 | 5 4 |
The A group course of disease the shortest March, the longest 5 years, average course of disease 2.4 years; The B group course of disease is the shortest 3.2 months, the longest 4.1 years, average course of disease 2.2 years.Two groups of courses of disease distribute and contrast, through X 2 test, and X
2=1.519, P＞0.05, difference does not have the significance meaning, and comparability is arranged.
The state of an illness distributes
Table 4a 60 routine chronic simple laryngitis state of an illness grades distribute
| Group | Total routine number | Slightly (%) | Moderate (%) | Severe (%) |
| A group B group | 30 30 | 11(36.7) 9(30.0) | 17(56.7) 16(53.3) | 2(6.6) 5(16.7) |
Two groups of state of an illness grades distribute and contrast, through X 2 test, and X
2=1.516, P＞0.05, difference does not have the significance meaning, and comparability is arranged.
The pharyngeal different sense disease state of an illness grade of table 4b 60 examples distributes
| Group | Total routine number | Slightly (%) | Moderate (%) | Severe (%) |
| A group B group | 30 30 | 6(20.0) 9(30.0) | 17(56.7) 16(53.3) | 7(23.3) 5(16.7) |
Two groups of state of an illness grades distribute and contrast, through X 2 test, and X
2=0.964, P＞0.05, difference does not have the significance meaning, and comparability is arranged.
Therapeutic outcome
General curative effect
Table 5a 60 routine chronic simple laryngitis efficacy analysis
| Group | Total routine number | Recovery from illness (%) | Produce effects (%) | Effectively (%) | Invalid (%) |
| A group B group | 30 30 | 7(23.4) 1(3.3) | 10(33.3) 8(26.7) | 10(33.3) 13(43.3) | 3(10.0) 8(26.7) |
Two groups of Comparison of therapeutic, through the Ridit check, u=2.4373, P＜0.05, meaning that there was a significant difference, A group curative effect is better than the B group.
The pharyngeal different sense disease efficacy analysis of table 5b 60 examples
| Group | Total routine number | Recovery from illness (%) | Produce effects (%) | Effectively (%) | Invalid (%) |
| A group B group | 30 30 | 9(30.0) 1(3.3) | 7(23.3) 8(26.7) | 12(40.0) 14(46.7) | 2(6.7) 7(23.3) |
Two groups of Comparison of therapeutic, through the Ridit check, u=2.4671, P＜0.05, meaning that there was a significant difference, A group curative effect is better than the B group.
Symptom (sign) integration changes situation
Table 7a 60 routine chronic simple laryngitis cardinal symptom (sign) integrations change to be analyzed
| Hoarse between sound | It is white sticking to cough up phlegm | The swelling of sound system | Itching of the throat pain | ||
| The A group | Integration is treated back integration therapeutic index (%) before treating | 125 48 61.6 | 132 51 61.4 | 136 53 61.0 | 129 49 62.0 |
| Contrast | Integration is treated the back integration before treating | 119 52 | 124 57 | 126 61 | 117 53 |
| Group | Therapeutic index (%) | 56.3 | 54.0 | 51.6 | 54.7 |
| X 2Value P value | 0.707 ＞0.05 | 1.409 ＞0.05 | 2.004 ＞0.05 | 1.068 ＞0.05 | |
As seen from the above table, before A group is treated with B group cardinal symptom, treat the back integration and change relatively there was no significant difference, P＞0.05.
The pharyngeal different sense disease symptom integral of table 7b 60 examples changes situation analysis
| The foreign body in pharynx sense | Breast side of body feeling of distension and oppression | The belch singultus | Irritated irritability | Insomnia and dreamful sleep | ||
| The A group | Integration is treated back integration therapeutic index (%) before treating | 127 51 59.8 | 76 29 61.8 | 79 32 59.5 | 68 25 63.2 | 65 25 61.5 |
| Matched group | Integration is treated back integration therapeutic index (%) before treating | 114 66 42.1 | 72 32 55.6 | 71 33 53.5 | 67 38 43.3 | 66 27 59.1 |
| X 2Value P value | 6.873 ＜0.05 | 0.372 ＞0.05 | 1.151 ＞0.05 | 4.626 ＜0.05 | 0.012 ＞0.05 | |
As seen from the above table, A organizes with the sense of B group cardinal symptom foreign body in pharynx, irritated irritability, the insomnia and dreamful sleep treatment is preceding, treatment back integration changes relatively significant difference, P＜0.05.
Untoward reaction: during the clinical verification, have no adverse reaction.
Experiment conclusion
The Jinsangliyan capsule is made up of Chinese medicines such as Poria, Rhizoma Pinelliae Preparatum, Arisaema Cum Bile, Cortex Magnoliae Officinalis, Periostracum Cicadaes, has the effect of drying dampness to eliminate phlegm, depressed liver-energy dispersing and QI regulating, relieving sore throat to recover voice.Result: the Jinsangliyan capsule for treating chronic simple laryngitis of aqueous extraction-alcohol precipitation technology preparation, 7 examples of wherein fully recovering, produce effects 10 examples, effective 10 examples, invalid 3 examples, total effective rate 90.0%; The Jinsangliyan capsule for treating chronic simple laryngitis of extraction process by water preparation, 1 example of wherein fully recovering, produce effects 8 examples, effective 13 examples, invalid 8 examples, total effective rate 73.3%; Two groups of Comparison of therapeutic, through the Ridit check, P＜0.05, difference has the significance meaning, and aqueous extraction-alcohol precipitation technology group curative effect is better than the extraction process by water group.The pharyngeal different sense disease of Jinsangliyan capsule for treating of water extract-alcohol precipitation preparation, 9 examples of wherein fully recovering, produce effects 7 examples, effective 12 examples, invalid 2 examples, total effective rate 93.3%; The pharyngeal different sense disease of Jinsangliyan capsule for treating of extraction process by water preparation, 1 example of wherein fully recovering, produce effects 8 examples, effective 14 examples, invalid 7 examples, total effective rate 76.7%; Two groups of Comparison of therapeutic, through the Ridit check, P＜0.05, difference has the significance meaning, and aqueous extraction-alcohol precipitation technology group curative effect is better than the extraction process by water group.
Prescription: Poria 165g, Fructus Aurantii Immaturus (stir-fry) 165g, Arisaema Cum Bile 165g, Fructus Amomi 165g, Semen Arecae 165g, Massa Medicata Fermentata (stir-fry) 165g, Rhizoma Zingiberis Recens 24.8g, Semen Oroxyli 165g, Rhizoma Pinelliae Preparatum 165g, Pericarpium Citri Reticulatae Viride (stir-fry) 165g, tangerine 165g, Fructus Amomi Rotundus 82.5g, Cortex Albiziae 165g, Caulis Perillae 165g, Periostracum Cicadae 165g, Cortex Magnoliae Officinalis (system) 165g;
The processing step of embodiment 1 preparation tablet is as follows:
Get Poria, Fructus Amomi, the Arisaema Cum Bile of recipe quantity 50%, be ground into fine powder, sieve, mixing, standby; Surplus after the pulverizing of Rhizoma Pinelliae Preparatum, Fructus Aurantii Immaturus, Pericarpium Citri Reticulatae Viride, dried tangerine peel, Fructus Amomi Rotundus, Semen Arecae, Cortex Albiziae, Massa Medicata Fermentata, Caulis Perillae, Rhizoma Zingiberis Recens, Periostracum Cicadae, Semen Oroxyli, Cortex Magnoliae Officinalis and Poria, Fructus Amomi, Arisaema Cum Bile three flavor medicines is added 10 times, 8 times decoctings boil secondary, each 2 hours, collecting decoction, filter, filtrate is concentrated into the clear paste that relative density is 1.15～1.20 (60 ℃), add ethanol and make pure content reach 60%, standing over night is got supernatant; Reclaim ethanol, being evaporated to relative density is the thick paste of 1.28～1.32 (60 ℃), adds the fine powder and the appropriate amount of auxiliary materials mixing of preparation, and compacting is in blocks, the bag film-coat, promptly.
The processing step of embodiment 2 preparation capsules is as follows:
Get Poria, Fructus Amomi, the Arisaema Cum Bile of recipe quantity 50%, be ground into fine powder, sieve, mixing, standby; Surplus after the pulverizing of Rhizoma Pinelliae Preparatum, Fructus Aurantii Immaturus, Pericarpium Citri Reticulatae Viride, dried tangerine peel, Fructus Amomi Rotundus, Semen Arecae, Cortex Albiziae, Massa Medicata Fermentata, Caulis Perillae, Rhizoma Zingiberis Recens, Periostracum Cicadae, Semen Oroxyli, Cortex Magnoliae Officinalis and Poria, Fructus Amomi, Arisaema Cum Bile three flavor medicines is added 10 times, 8 times decoctings boil secondary, each 2 hours, collecting decoction, filter, filtrate is concentrated into the clear paste that relative density is 1.15～1.20 (60 ℃), add ethanol and make pure content reach 60%, standing over night is got supernatant; Reclaim ethanol, being evaporated to relative density is the thick paste of 1.28～1.32 (60 ℃), adds the fine powder and the appropriate amount of auxiliary materials mixing of preparation, and drying is pulverized, and makes fine particle, incapsulates, promptly.
The processing step of embodiment 3 preparation granules is as follows:
Get Poria, Fructus Amomi, the Arisaema Cum Bile of recipe quantity 50%, be ground into fine powder, sieve, mixing, standby; Surplus after the pulverizing of Rhizoma Pinelliae Preparatum, Fructus Aurantii Immaturus, Pericarpium Citri Reticulatae Viride, dried tangerine peel, Fructus Amomi Rotundus, Semen Arecae, Cortex Albiziae, Massa Medicata Fermentata, Caulis Perillae, Rhizoma Zingiberis Recens, Periostracum Cicadae, Semen Oroxyli, Cortex Magnoliae Officinalis and Poria, Fructus Amomi, Arisaema Cum Bile three flavor medicines is added 10 times, 8 times decoctings boil secondary, each 2 hours, collecting decoction, filter, filtrate is concentrated into the clear paste that relative density is 1.15～1.20 (60 ℃), add ethanol and make pure content reach 60%, standing over night is got supernatant; Reclaim ethanol, being evaporated to relative density is the thick paste of 1.28～1.32 (60 ℃), adds the fine powder and the appropriate amount of auxiliary materials mixing of preparation, and drying is pulverized, and makes fine particle, and packing promptly.
The processing step of embodiment 4 preparation soft capsules is as follows:
Get Poria, Fructus Amomi, the Arisaema Cum Bile of recipe quantity 50%, be ground into fine powder, sieve, mixing, standby; Surplus after the pulverizing of Rhizoma Pinelliae Preparatum, Fructus Aurantii Immaturus, Pericarpium Citri Reticulatae Viride, dried tangerine peel, Fructus Amomi Rotundus, Semen Arecae, Cortex Albiziae, Massa Medicata Fermentata, Caulis Perillae, Rhizoma Zingiberis Recens, Periostracum Cicadae, Semen Oroxyli, Cortex Magnoliae Officinalis and Poria, Fructus Amomi, Arisaema Cum Bile three flavor medicines is added 10 times, 8 times decoctings boil secondary, each 2 hours, collecting decoction, filter, filtrate is concentrated into the clear paste that relative density is 1.15～1.20 (60 ℃), add ethanol and make pure content reach 60%, standing over night is got supernatant; Reclaim ethanol, being evaporated to relative density is the thick paste of 1.28～1.32 (60 ℃), adds the fine powder and the appropriate amount of auxiliary materials mixing of preparation, and drying is pulverized, and makes fine particle, adds vegetable oil, makes soft capsule promptly.
Embodiment 5 should invent the application Poria 165g in the medicine of treatment chronic simple laryngitis, pharyngeal different sense disease, Fructus Aurantii Immaturus (stir-fry) 165g, Arisaema Cum Bile 165g, Fructus Amomi 165g, Semen Arecae 165g, Massa Medicata Fermentata (stir-fry) 165g, Rhizoma Zingiberis Recens 24.8g, Semen Oroxyli 165g, Rhizoma Pinelliae Preparatum 165g, Pericarpium Citri Reticulatae Viride (stir-fry) 165g, tangerine 165g, Fructus Amomi Rotundus 82.5g, Cortex Albiziae 165g, Caulis Perillae 165g, Periostracum Cicadae 165g, Cortex Magnoliae Officinalis (system) 165g
Make 1000, the 0.4g/ grain, the patient of trouble chronic simple laryngitis, pharyngeal different sense disease, oral, each 2-4 grain, every day 2 times.
Claims (5)
1, a kind of Chinese medicine preparation technology who treats chronic simple laryngitis, laryngopharyngeal paresthesia is characterized in that this method is:
Get Poria, Fructus Amomi, the Arisaema Cum Bile of recipe quantity 40%-60%, be ground into fine powder, sieve, mixing, standby; With surplus after the pulverizing of Rhizoma Pinelliae Preparatum, Fructus Aurantii Immaturus, Pericarpium Citri Reticulatae Viride, dried tangerine peel, Fructus Amomi Rotundus, Semen Arecae, Cortex Albiziae, Massa Medicata Fermentata, Caulis Perillae, Rhizoma Zingiberis Recens, Periostracum Cicadae, Semen Oroxyli, Cortex Magnoliae Officinalis and Poria, Fructus Amomi, Arisaema Cum Bile three flavor medicines add 8-12 doubly, a 6-10 times decocting boils secondary, each 1-3 hour, collecting decoction, filter, in the time of 50-70 ℃, it is 1.15～1.20 clear paste that filtrate is concentrated into relative density, adds ethanol and makes pure content reach 50-70%, standing over night is got supernatant; Reclaim ethanol, in the time of 50-70 ℃, be evaporated to relative density and be 1.28～1.32 thick paste, add the fine powder mixing of preparation, drying is pulverized, and makes fine particle, promptly.
2, a kind of Chinese medicine preparation technology who treats chronic simple laryngitis, laryngopharyngeal paresthesia as claimed in claim 1 is characterized in that this method is:
Get Poria, Fructus Amomi, the Arisaema Cum Bile of recipe quantity 50%, be ground into fine powder, sieve, mixing, standby; Surplus after the pulverizing of Rhizoma Pinelliae Preparatum, Fructus Aurantii Immaturus, Pericarpium Citri Reticulatae Viride, dried tangerine peel, Fructus Amomi Rotundus, Semen Arecae, Cortex Albiziae, Massa Medicata Fermentata, Caulis Perillae, Rhizoma Zingiberis Recens, Periostracum Cicadae, Semen Oroxyli, Cortex Magnoliae Officinalis and Poria, Fructus Amomi, Arisaema Cum Bile three flavor medicines is added 10 times, 8 times decoctings boil secondary, each 2 hours, collecting decoction, filter, in the time of 60 ℃, it is 1.15～1.20 clear paste that filtrate is concentrated into relative density, adds ethanol and makes pure content reach 60%, standing over night is got supernatant.Reclaim ethanol, in the time of 60 ℃, be evaporated to relative density and be 1.28～1.32 thick paste, add the fine powder and the appropriate amount of auxiliary materials mixing of preparation, drying is pulverized, and makes fine particle, promptly.
3, a kind of application for the treatment of the Chinese medicine of chronic simple laryngitis, laryngopharyngeal paresthesia is characterized in that this Chinese medicine preparation has phlegm-dispelling functions, can promote the tracheal glands secretion; Inhibited to the chronic inflammatory disease granulation tissue hyperplasia.
4, a kind of application for the treatment of the Chinese medicine of chronic simple laryngitis, laryngopharyngeal paresthesia as claimed in claim 3 is characterized in that the application of this Chinese medicine preparation in treatment chronic simple laryngitis, pharyngeal different sense disease drug.
5,, it is characterized in that this Chinese medicine preparation can be made into any in tablet, capsule, granule, the soft capsule as the described a kind of application for the treatment of the Chinese medicine of chronic simple laryngitis, laryngopharyngeal paresthesia of claim 3-4.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102363036A (en) * | 2011-10-26 | 2012-02-29 | 西安碑林药业股份有限公司 | Chinese medicinal composition for clearing throat and pharynx, and preparation method thereof |
| WO2017083363A1 (en) * | 2015-11-09 | 2017-05-18 | Unigen, Inc. | Natural preservatives and antimicrobial agents, including compositions thereof |
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2006
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102363036A (en) * | 2011-10-26 | 2012-02-29 | 西安碑林药业股份有限公司 | Chinese medicinal composition for clearing throat and pharynx, and preparation method thereof |
| WO2017083363A1 (en) * | 2015-11-09 | 2017-05-18 | Unigen, Inc. | Natural preservatives and antimicrobial agents, including compositions thereof |
| US10780173B2 (en) | 2015-11-09 | 2020-09-22 | Unigen, Inc. | Natural preservatives and antimicrobial agents, including compositions thereof |
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