CN1896739A - Multi-component enzymelinked immune inspection reagent kit of residual beast medicine - Google Patents
Multi-component enzymelinked immune inspection reagent kit of residual beast medicine Download PDFInfo
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- CN1896739A CN1896739A CN 200510010913 CN200510010913A CN1896739A CN 1896739 A CN1896739 A CN 1896739A CN 200510010913 CN200510010913 CN 200510010913 CN 200510010913 A CN200510010913 A CN 200510010913A CN 1896739 A CN1896739 A CN 1896739A
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Abstract
This invention discloses a many components enzyme linked detecting test box to detect residual beast medicine, it is composed of carrier box and enzyme batten, the carrier box contains carrier box cover plate and the carrier box rear panel, the enzyme batten contains the enzyme board hole, the carrier box sets up the carrier slot that depositing the enzyme batten in different color or sign mark. This detecting test box can detect many components (include many categories) residual beast medicine, not only detecting many components, many categories residual beast medicine rapidly at the same time, in the scene, in the living body , it can also provide the specific residual numerical value and the super-standard condition of various components and categories beast medicine in animal source food. It is applied to the various levels beast medicine and feed supervise institute, import and export quarantine bureau and large-scale food produce enterprise to detect and control the content of residual beast medicine in animal source food.
Description
Technical field
The present invention relates to a kind of multi-component enzymelinked immune inspection reagent kit of residual beast medicine, particularly relate to the enzyme linked immunological kit of a kind of parallel joint-detection such as multiple veterinary drug components such as Clenbuterol, sulfamido, chloromycetin, terramycin, diethylstilbestrol and Enrofloxacin.
Background technology
The analyzing and testing of residual animal medicine is the important means that guarantees foodsafety in the food.The residue of veterinary drug analytical technology is the trace analysis technology in the complex mixture, present stage, gas chromatography (GC), high performance liquid chromatography (HPLC) reach and mass spectrum (MS) coupling instrumental method analytical technologies such as (GC/MS, LC/MS) is the main means of residual animal medicine analysis and quantitative measurement.Instrumental method is quantitatively accurate, highly sensitive, but it is strongly professional, need expensive huge equipment and long sample preparation time, expend time in long, expense is high, is difficult for being grasped by the staff of grass-roots unit, regular field quick detection in the animal products production and sales system of be not suitable on a small scale, disperseing, often analysis result comes out, and meat milk egg etc. is eaten already.For food such as the meat that prevents high residual hazard, milk, egg enter resident's dining table, need a kind of quick, easy, safe, reliable, wield detection technique of grass-roots unit.、
Enzyme immunoassay analysis (enzyme immunoassays, EIA) be to utilize the specificity combination of antigen and antibody to come selectivity identification and mensuration to can be used as the method for the determinand of antibody or antigen, because other chaff interference that exists in the sample can not produce immunity identification, therefore immunoassay has high specific and high resolving power, uses very extensive.
Using at present maximum in the residue of veterinary drug analysis is enzyme linked immunological absorption detection technique (enzyme-linked immunosorbent Assay, ELISA), promptly by on suitable carriers, the enzymic-labelled antibody binding site of the limited amount antigen of enzyme labeling and unknown antigen (veterinary drug) competition insolubilized antibody binding site or solid phase antigen and unknown antigen competition limited amount forms antigen antibody complex.Under certain substrate participated in, the substrate for enzymatic activity on the compound made its hydrolysis, oxidation or be reduced into another kind of band look product.According to the appearance of coloured product, objectively respond the existence of enzyme, i.e. the existence of enzyme-labelled antigen-antibody or enzyme labelled antibody-antigenic compound.Because the degraded substrate level of enzyme is directly proportional with the colour developing degree, by observing or spectrophotometric determination, can qualitative or detection by quantitative unknown antigen.
Advantages such as sampling amount is few, pre-treatment is simple, capacity is big though immunoassay technology has, the instrumentation degree is low, analysis cost is low, efficient is high, sensitivity, but ELISA also exists some defectives, very high as selectivity to reagent, the compound of similar is had to a certain degree cross reaction, the compound that the analyzing molecules amount is very little or very unstable compounds certain difficulty is arranged; In view of veterinary drug various in style, residual animal medicine in animal derived food may be for residual in the various ingredients veterinary drug, the shortcoming of the maximum of existing commercial residue of veterinary drug ELISA kit just is that at present such all kits all can only be analyzed the single component residual animal medicine of (containing single classification), and can not be at the scene, at live body, at one time in the residual animal medicine of express-analysis detection various ingredients, plurality of classes.
Summary of the invention
The objective of the invention is to provide a kind of multi-component enzymelinked immune inspection reagent kit of residual beast medicine, but fast detecting polycomponent (containing multi-class) residual animal medicine (as Clenbuterol, sulfamido, chloromycetin, terramycin, diethylstilbestrol and Enrofloxacin etc.), by parallel joint-detection, not only can be at the scene, at live body, at one time in express-analysis detect the residual animal medicine of various ingredients, plurality of classes, various components, various types of other veterinary drug concrete residual quantity numerical value and situation that exceeds standard in animal derived food can also be provided.Be applicable to veterinary drugs at different levels and feed supervision institute, import and export of detection and the monitoring of units such as inspection and quarantine bureau and large-scale food production processing enterprise residual animal medicine in the animal derived food.
For achieving the above object, the technical solution used in the present invention is: this multi-component enzymelinked immune inspection reagent kit of residual beast medicine is by carrier box, ELIAS strip and matched reagent are formed, described carrier box contains carrier box cover plate and carrier box backboard, be provided with the carrier channels that to place ELIAS strip in the carrier box backboard, ELIAS strip is made of 8 holes or ELISA Plate hole, 12 hole, all be surrounded by suitable antigen (as Clenbuterol etc.) or antibody (as the antibody of anti-clenbuterol etc.) in its every hole, matched reagent comprises sample pre-treatments reagent, enzyme len antibody or enzyme associated antigen, standard specimen, cleaning fluid, substrate solution and stop buffer, be provided with 12 above carrier channels in the carrier box backboard, be placed with respectively in the carrier channels and can detect 2 to 12 kinds of polycomponent residual animal medicines (as Clenbuterol, sulfamido, chloromycetin, terramycin, six components such as diethylstilbestrol and Enrofloxacin), ELIAS strip with different colours or marked.
Can also set up in the carrier channels in the described carrier box backboard more than 12, can detect the ELIAS strip of other component residual animal medicine.
Multi-component enzymelinked immune inspection reagent kit of residual beast medicine of the present invention can utilize that the polycomponent immunoassay technology is integrated, the principle of high flux, parallelization, with these each components or ELIAS strip of all categories with the parallel mode combination in any, integrate, can the disposable various ingredients residual animal medicine that detects simultaneously in the animal derived food.By such kit of a large amount of employings, detect when can realize a large amount of sample polycomponent residual animal medicine, not only improved the operation inconvenience that is had when instrumental methods such as adopting HPLC is used for detection of veterinary drugs in food, time-consuming, it is not enough to cost an arm and a leg etc., and than and comprehensively from abundant information that the one-component enzyme-linked immunologic detecting kit obtained, can realize the disposable residual animal medicine that detects various ingredients, therefore in veterinary drugs at different levels and feed supervision institute, import and export inspection and quarantine bureau and large-scale food production processing enterprise etc. and carry out having very high use value in the detection of residual animal medicine in the animal derived food and the monitoring.
Below in conjunction with accompanying drawing the embodiment of the invention is done further statement.
Description of drawings
Fig. 1 is the structural representation of the carrier box of multi-component enzymelinked immune inspection reagent kit of residual beast medicine.
Fig. 2 is the structural representation of the ELIAS strip of multi-component enzymelinked immune inspection reagent kit of residual beast medicine.
Fig. 3 is the structural representation of the base of residue of veterinary drug polycomponent enzyme linked immunological kit carrier box.
Embodiment
Fig. 1, among Fig. 2 and Fig. 3, multi-component enzymelinked immune inspection reagent kit of residual beast medicine of the present invention is made up of carrier box 1 and ELIAS strip 5, carrier box 1 contains carrier box cover plate 2 and carrier box backboard 3, carrier box backboard 3 is rectangle, be provided with the carrier channels 4 that to place 12 (also can be more) ELIAS strip 5 in the carrier box 1, ELIAS strip 5 comprises 8 or 12 ELISA Plate holes 6, all be coated with suitable antigen or antibody in its every hole, in the carrier channels 4 as required, at least can place respectively and can detect 2 to 12 kinds of polycomponent residual animal medicines (as Clenbuterol, sulfamido, chloromycetin, terramycin, six components such as diethylstilbestrol and Enrofloxacin), ELIAS strip 5 with different colours or marked, be used for detecting simultaneously animal derived food, as meat, polycomponent residual animal medicine in the food such as milk and aquatic products category.
During use, samples such as the meat that will detect, milk and aquatic products are after the sample pre-treatments agent treated, requirement to specifications, the enzyme labelled antibody that in ELISA Plate hole 6, adds testing sample and suitable concn simultaneously, in other plate holes of same ELIAS strip 6, add and add the standard specimen of variable concentrations and the enzyme labelled antibody of suitable concn simultaneously, under 37 ℃, hatched 1 hour, add the substrate solution colour developing, after adding the stop buffer cessation reaction again, colour developing situation that can visual inspection ELISA Plate hole 6 is carried out qualitative detection, or places microplate reader to carry out reading to carry out detection by quantitative.
To detect the Clenbuterol in the pork simultaneously, sulfamido, chloromycetin, terramycin, 6 kinds of residual animal medicines such as diethylstilbestrol and Enrofloxacin are example, with the pork sample respectively after the sample pre-treatments agent treated, 1# in 1# ELIAS strip 6, all add 50 μ l testing samples and 100 μ l anti-clenbuterol antibody in the 2# plate hole simultaneously with enzyme labeling, in 3# to 8# plate hole, all add the Clenbuterol standard specimen of 50 μ l variable concentrations and the anti-clenbuterol antibody of 100 μ l enzyme labelings simultaneously, in like manner, 2#, 3#, 4#, standard specimen and enzymic-labelled antibody corresponding in 5# and the 6# ELIAS strip 6 are respectively sulfamido standard specimen/anti-sulfamido enzymic-labelled antibody, chloromycetin standard specimen/chloramphenicol resistance enzymic-labelled antibody, terramycin standard specimen/anti-terramycin enzymic-labelled antibody, diethylstilbestrol standard specimen/anti-diethylstilbestrol enzymic-labelled antibody and Enrofloxacin standard specimen/anti-Enrofloxacin enzymic-labelled antibody.Afterwards, these 6 ELIAS strip 6 are placed identical carrier groove 4, after hatching 1 hour under 37 ℃, add the substrate solution colour developing, after adding the stop buffer cessation reaction again, can place microplate reader to carry out assay readings, can find the concentration value of 1# and the corresponding testing sample of 2# according to 3# to 8# standard specimen colour developing graticule that situation is done in every ELIAS strip 6.So just can detect the residual concentration value of 6 kinds of residual animal medicines such as Clenbuterol, sulfamido, chloromycetin, terramycin, diethylstilbestrol and Enrofloxacin in the pork sample once simultaneously.
Should be understood that these embodiment only to be used to the present invention is described and be not used in and limit the scope of the invention.The implementation method of unreceipted actual conditions in the foregoing description, usually according to the condition described in normal condition such as the laboratory manual, or the condition of advising according to manufacturer.
Claims (2)
1, a kind of multi-component enzymelinked immune inspection reagent kit of residual beast medicine, it is characterized in that: it is made up of carrier box (1) and ELIAS strip (5), carrier box (1) contains carrier box cover plate (2) and carrier box backboard (3), ELIAS strip (5) includes 8 or 12 ELISA Plate holes (6), be provided with in the carrier box (comprise 12) more than 12 and deposit can detect 2～12 kinds of polycomponent residual animal medicines, with the carrier channels (4) of the ELIAS strip (5) of different colours or marked.
2, according to claims 1 described multi-component enzymelinked immune inspection reagent kit of residual beast medicine, it is characterized in that: can also set up in the carrier channels (4) in the described carrier box backboard (3) more than 12, can detect the ELIAS strip (5) of other component residual animal medicine.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510010913 CN1896739A (en) | 2005-07-15 | 2005-07-15 | Multi-component enzymelinked immune inspection reagent kit of residual beast medicine |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510010913 CN1896739A (en) | 2005-07-15 | 2005-07-15 | Multi-component enzymelinked immune inspection reagent kit of residual beast medicine |
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| CN1896739A true CN1896739A (en) | 2007-01-17 |
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| CN 200510010913 Pending CN1896739A (en) | 2005-07-15 | 2005-07-15 | Multi-component enzymelinked immune inspection reagent kit of residual beast medicine |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102012424A (en) * | 2009-09-08 | 2011-04-13 | 宁波博奥生物工程有限公司 | Chip kit for quantitative detection of veterinary drug residue |
| CN108387725A (en) * | 2018-01-13 | 2018-08-10 | 中国医学科学院医学生物学研究所 | A kind of I type of poliovirus, II type, the pre-coated associated detecting method of III type D antigens and its detection kit and application |
| CN110261553A (en) * | 2019-07-15 | 2019-09-20 | 北京中孚豹科技有限公司 | A kind of method and system of quick detection food veterinary drug residue and violated addition |
-
2005
- 2005-07-15 CN CN 200510010913 patent/CN1896739A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102012424A (en) * | 2009-09-08 | 2011-04-13 | 宁波博奥生物工程有限公司 | Chip kit for quantitative detection of veterinary drug residue |
| CN108387725A (en) * | 2018-01-13 | 2018-08-10 | 中国医学科学院医学生物学研究所 | A kind of I type of poliovirus, II type, the pre-coated associated detecting method of III type D antigens and its detection kit and application |
| CN110261553A (en) * | 2019-07-15 | 2019-09-20 | 北京中孚豹科技有限公司 | A kind of method and system of quick detection food veterinary drug residue and violated addition |
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