CN1887220A - Dispersion compensating method and system for optically coherent chromatographic imaging - Google Patents
Dispersion compensating method and system for optically coherent chromatographic imaging Download PDFInfo
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Abstract
The present invention discloses dispersion compensating method and system for optically coherent tomographic imaging (OCT). The system has one increased blazed grating parallelly set with the original blazed grating in a single grating fast scanning optical delay line, and thus one independently regulated variable of interval between two blazed gratings and capacity of generating group velocity dispersion and third-order dispersion in great varying range and arbitrary sign combination, so that the OCT system may obtain precise matching between the dispersion of the reference arm and the dispersion of the sample arm and longitudinal resolution approaching the theoretical calculated value. The double grating system has wide dispersion regulating range, wide compensation spectrum range and small residual dispersion other than the capacity of independently controlling phase delay and group delay, and possesses three functions of depth scan, phase modulation and dispersion compensation.
Description
Technical field
The present invention relates to a kind of optical coherent chromatographic imaging (OCT) technology, relate in particular to dispersion compensation method and system in a kind of optical coherent chromatographic imaging.
Background technology
Optical coherent chromatographic imaging (Optical Coherence Tomography, be called for short OCT) be a kind of biomedical optical image technology with chromatography function, possessed the required ultrahigh resolution (1 micron to 20 microns) of histopathological analysis, can non-invasively carry out visual imaging to the internal structure below the tissue surface, physiological function and even molecular information, be that important minimum is invaded imaging tool, can bring into play clinical function such as early diagnosis, process monitoring and operation mediation.OCT is with its ultrahigh resolution, ultrafast image taking speed, radiationless damage, optical information diversity, moderate cost, compact conformation, and with the compatible advantage such as good of existing Medical Instruments, be at present by the extensively good optical instrument that can on clinical medicine, play a significant role.
In structure OCT and various function OCT system, all need to introduce Doppler frequency shift and realize heterodyne modulation.Doppler frequency shift is normally realized by introducing electric light or acousto-optic modulator in reference arm.Yet electric light in the manipulator or acousto-optic crsytal have been introduced bigger positive dispersion, if positive dispersion unnecessary in the reference arm is not compensated, will make OCT system longitudinal resolution not reach desired theoretical value.At present, monochromatic light grid rapid scanning delay line (Rapid Scanning Optical Delay Line, be called for short RSOD) be widely used Dispersion Compensation Systems among the OCT, as shown in Figure 1, the directional light that is penetrated by fiber optic collimator mirror 1 is behind balzed grating, 2 diffraction, the diffraction of light angle of different wave length is different, through behind the fourier transform lens 3, the light of each wavelength all focuses on the galvanometer 4, the light of each wavelength is through galvanometer 4 reflections, once more by behind the fourier transform lens 3, the light of each wavelength all will with the parallel light that behind balzed grating, 2 diffraction, incides the identical wavelength on the fourier transform lens 3, but the light direction of different wave length is different, again behind balzed grating, 2 diffraction, the diffraction light of various different wave lengths will be parallel to each other, but the light of different wave length has a transversion malposition, and the light of each wavelength returns former road after flat mirror reflects, eliminates the transversion malposition between each wavelength light, become a branch of light, enter the fiber optic collimator mirror.Monochromatic light grid RSOD introduces chromatic dispersion by regulating balzed grating, with respect to depart from (being defocusing amount) of fourier transform lens focus.But GVD (GVD) and third-order dispersion (TOD) contrary sign that the method is introduced, can't compensate positive GVD and TOD that above-mentioned crystal is introduced simultaneously, promptly system dispersion can only be matched second order, but still there is serious third-order dispersion mismatch, makes the actual longitudinal resolution of system be lower than theoretical value because of the chromatic dispersion mismatch.
Summary of the invention
The purpose of this invention is to provide dispersion compensation method and system in a kind of optical coherent chromatographic imaging, this method can realize the GVD (GVD) and the third-order dispersion (TOD) of the optional sign combination of big excursion, the chromatic dispersion of OCT system can be matched three rank, thereby the longitudinal resolution of guaranteeing the OCT system does not descend because of the chromatic dispersion mismatch.
The objective of the invention is to be achieved through the following technical solutions:
1. the dispersion compensation method in the optical coherent chromatographic imaging: it is parallel to increase a balzed grating, and the two balzed grating, grooves with the parallel placement of original balzed grating, in original monochromatic light grid rapid scanning delay line newly, the directional light that is penetrated by optical fiber collimator at first arrives on the balzed grating, that increases newly, and diffraction light arrives original balzed grating, again behind its diffraction.
The described balzed grating, that increases newly is positioned between optical fiber collimator and the original balzed grating,, such double balzed grating rapid scanning delay line structure has increased the grating space regulated variable newly, grating space and grating defocusing amount are regulated in combination, can produce the GVD and the third-order dispersion of optional sign combination, thereby the chromatic dispersion of reference arm and sample arm is accurately mated.
When the optical axis of the grating face of the balzed grating, of two parallel placements and fourier transform lens is vertical, only introduce static chromatic dispersion, promptly dispersion measure does not become in time, and does not have dynamic dispersion, promptly dispersion measure in time change and change; When the optical axis out of plumb of the grating face of the balzed grating, of two parallel placements and fourier transform lens, then not only introduce static chromatic dispersion but also introduce dynamic dispersion this moment, and the size of dynamic dispersion is by the angle decision of grating face normal and optical axis, and angle is big more, and it is big more to introduce dynamic dispersion.
When the grating face of two balzed grating,s has angle, or the groove direction of two balzed grating,s is when having angle, show according to Theoretical Calculation and numerical analysis, the all strict dispersion measure of introducing when parallel of the grating face that the dispersion measure of introducing this moment will depart from relative two balzed grating,s and groove direction, but still can produce the GVD and the third-order dispersion of optional sign combination, thereby the chromatic dispersion of reference arm and sample arm is accurately mated.
2. the Dispersion Compensation Systems in the optical coherent chromatographic imaging comprises by optical fiber collimator, balzed grating,, fourier transform lens, galvanometer and plane mirror.Between optical fiber collimator and original balzed grating,, increased another balzed grating, newly, this balzed grating, and the parallel placement of original balzed grating,, the grating face of parallel and two balzed grating,s of two balzed grating, grooves is perpendicular to the optical axis of fourier transform lens; Perhaps the optical axis with fourier transform lens is not vertical for the grating face of two balzed grating,s; Perhaps the grating face of two balzed grating,s is not parallel; Perhaps the groove direction of two balzed grating,s is not parallel; The directional light that is gone out by the fiber optic collimator mirror at first arrives the balzed grating, that increases newly, diffraction light arrives original balzed grating, and again by its diffraction behind its diffraction, the diffraction light of each wavelength focuses on the galvanometer by fourier transform lens, the light of each wavelength is through vibration mirror reflected, after passing through fourier transform lens again, successively by balzed grating, and balzed grating, diffraction, the light of various different wave lengths all will be parallel with former incident illumination this moment, the light of each wavelength incides on the plane mirror of a reflecting surface and the vertical placement of each wavelength light direct of travel, former then road is returned, the light of each wavelength is final to be overlapped to a branch of, enters the fiber optic collimator mirror.
When the grating face of two balzed grating,s is not vertical with the optical axis of fourier transform lens, this system will introduce dynamic dispersion, and the size of the dynamic dispersion of introducing is by the angle decision of grating face normal and optical axis, and angle is big more, and it is big more to introduce dynamic dispersion.
Principle of the present invention is: make original balzed grating, introduce minus GVD and positive third-order dispersion with respect to the defocusing amount of fourier transform lens; And the demand pairs by suitable selection two balzed grating,s, can make two balzed grating, spacings when introducing less positive GVD, introduce bigger minus third-order dispersion, total GVD and third-order dispersion that so suitable adjusting two chromatic dispersion regulated variables just can make double grating RSOD introduce are negative value, thus the positive dispersion that crystal is introduced in the compensation of phase manipulator.Also can be by selecting the demand pairs of two balzed grating,s, GVD and third-order dispersion that the optional sign that double grating RSOD is introduced makes up are to satisfy the concrete needs of using.
The present invention compares the beneficial effect that has with background technology:
1) in the monochromatic light grid RSOD of routine, increased a balzed grating, newly, this independently adjusts variable to have introduced two balzed grating, spacings, balzed grating, among itself and the conventional monochromatic light grid RSOD is regulated with respect to the defocusing amount combination of Fourier transform lens, can produce the GVD (GVD) and the third-order dispersion (TOD) of the optional sign combination of big excursion, the chromatic dispersion of reference arm and sample arm is accurately mated.Thereby based on the OCT system of monochromatic light grid RSOD dispersion compensation, longitudinal resolution is more near calculated value.Double grating RSOD has that the dispersion adjustment scope is big, compensated spectrum wide ranges, the little advantage of residual dispersion.
2) The theoretical analysis, analog computation and experimental demonstration, double grating RSOD also have the same independent control phase delay of conventional monochromatic light grid RSOD and the ability of group delay, therefore can bear depth scan, position modulation and three functions of dispersion compensation mutually.
Description of drawings
Fig. 1 is the structural representation of monochromatic light grid rapid scanning delay line conventional in the existing OCT system.
Fig. 2 is the structural representation of the double grating rapid scanning delay line of the present invention's proposition.
Among the figure: 1, optical fiber collimator, 2, balzed grating,, 3, fourier transform lens, 4, galvanometer, 5, plane mirror, 6, balzed grating.
The specific embodiment
Further specify the principle of the present invention and the specific embodiment below in conjunction with accompanying drawing.
The present invention increases a balzed grating, between the fiber optic collimator mirror of original conventional monochromatic light grid RSOD and original balzed grating,, it is consistent with parallel placement of original balzed grating, and grating line direction.As shown in Figure 2, double grating RSOD is made up of optical fiber collimator 1, balzed grating, 2, fourier transform lens 3, galvanometer 4, plane mirror 5 and balzed grating, 6.The low-coherent light of broadband spectral is penetrated by fiber optic collimator mirror 1, at first on balzed grating, 2 diffraction takes place.Subsequently, diffraction takes place in the light of each wavelength once more on balzed grating, 6, by fourier transform lens 3, the light of each wavelength will focus on the galvanometer 4, behind the light process fourier transform lens 3 of galvanometer 4 reflections, the light of each wavelength all will with the parallel light that behind balzed grating, 6 diffraction, incides the identical wavelength on the fourier transform lens 3, but the light direction of different wave length is different.The light of each wavelength more successively behind balzed grating, 6 and balzed grating, 2 diffraction the light of each wavelength will become directional light, but the light of different wave length has a transversion malposition.The light of each wavelength incides on the plane mirror 5 of a reflecting surface and the vertical placement of each wavelength light direct of travel, returns along former road then, and the light of each wavelength has finally been eliminated transversion malposition, overlaps to a branch of, enters fiber optic collimator mirror 1.The light beam of this moment has compensated the chromatic dispersion of being introduced by electric light in the phase-modulator or acousto-optic crsytal, interferes at the reflected light of 2*2 fiber coupler place and sample arm to produce interference signal.
Grating face with balzed grating, 2 and balzed grating, 6 is parallel, and the parallel and grating face of groove direction is that example is analyzed the chromatic dispersion that double grating RSOD introduces perpendicular to the optical axis of fourier transform lens 3.As shown in Figure 2, the path of the corresponding coloured light of trace in double grating RSOD, can obtain the phase place of this coloured light in double grating RSOD, and notice adding that introducing ground phase place improvement factor by two gratings can obtain this coloured light to deserved real phase place, this phase place is made Taylor series expansion to centre wavelength, the GVD that can obtain double grating RSOD introducing is determined by the defocusing amount of balzed grating, 6 relative fourier transform lenses 3 and the spacing of balzed grating, 2 and balzed grating, 6 all that with third-order dispersion wherein GVD and the third-order dispersion of being introduced by defocusing amount provided by following two formulas:
Wherein m represents that the level of glittering of balzed grating, 2 is inferior, and c represents the very aerial light velocity, L
1-f represents the defocusing amount of balzed grating, 2 relative fourier transform lenses 3, d
EffRepresent the equivalent grid stroke logarithm under the parallel placement situation of two balzed grating,s, θ represents the angle of diffraction of corresponding coloured light behind balzed grating, 2 diffraction.
GVD and the third-order dispersion introduced by two grating spaces are provided by following two formulas:
Wherein, m represents that the level of glittering of balzed grating, 6 is inferior, L
2The spacing of representing two balzed grating,s, d
1The grating constant of expression balzed grating, 6, β represents the angle of diffraction of corresponding coloured light behind balzed grating, 6 diffraction.
So total GVD and third-order dispersion that double grating RSOD introduces are:
GVD
total(ω)=GVD
I(ω)+GVD
II(ω) (5)
TOD
total(ω)=TOD
I(ω)+TOD
II(ω) (6)
When the β value makes 3tan
2During β-1 ≈ 0, just can be at GVD
IIGet less on the occasion of situation under obtain bigger minus TOD
IIIf make L this moment
1-f>0, then GVD
I<0, TOD
I>0.Because I depends on different variablees with II, can control the value of two variablees respectively, make GVD
Total<0 and TOD
Total<0, this just can compensate simultaneously to positive GVD and the TOD that crystal in the reference arm is introduced.
In the chromatic dispersion matching process, the chromatic dispersion amount of not matching according to reference arm and sample arm, suitably regulate balzed grating, 2 and the spacing of balzed grating, 6 and the defocusing amount of balzed grating, 2 relative fourier transform lenses 3, just can make that the GVD of reference arm and sample arm and third-order dispersion are accurately mated, thereby the longitudinal resolution of guaranteeing the OCT system does not descend because of the chromatic dispersion mismatch.
Claims (6)
1. the dispersion compensation method in the optical coherent chromatographic imaging, it is characterized in that: it is parallel to increase a balzed grating, and the two balzed grating, grooves with the parallel placement of original balzed grating, in original monochromatic light grid rapid scanning delay line newly, the directional light that is penetrated by optical fiber collimator at first arrives on the balzed grating, that increases newly, and diffraction light arrives original balzed grating, again behind its diffraction.
2. the dispersion compensation method in the optical coherent chromatographic imaging according to claim 1, it is characterized in that: the described balzed grating, that increases newly is positioned between optical fiber collimator and the original balzed grating,, such double balzed grating rapid scanning delay line structure has increased the grating space regulated variable newly, grating space and grating defocusing amount are regulated in combination, can produce the GVD and the third-order dispersion of optional sign combination, thereby the chromatic dispersion of reference arm and sample arm is accurately mated.
3. the dispersion compensation method in the optical coherent chromatographic imaging according to claim 1 and 2, it is characterized in that: when the optical axis of the grating face of the balzed grating, of two parallel placements and fourier transform lens is vertical, only introduce static chromatic dispersion, be that dispersion measure does not become in time, and do not have dynamic dispersion, promptly dispersion measure in time change and change; When the optical axis out of plumb of the grating face of the balzed grating, of two parallel placements and fourier transform lens, then not only introduce static chromatic dispersion but also introduce dynamic dispersion this moment, and the size of dynamic dispersion is by the angle decision of grating face normal and optical axis, and angle is big more, and it is big more to introduce dynamic dispersion.
4. the dispersion compensation method in the optical coherent chromatographic imaging according to claim 1 and 2, it is characterized in that: when the grating face of two balzed grating,s has angle, or the groove direction of two balzed grating,s is when having angle, show according to Theoretical Calculation and numerical analysis, the all strict dispersion measure of introducing when parallel of the grating face that the dispersion measure of introducing this moment will depart from relative two balzed grating,s and groove direction, but still can produce the GVD and the third-order dispersion of optional sign combination, thereby the chromatic dispersion of reference arm and sample arm is accurately mated.
5. the Dispersion Compensation Systems in the optical coherent chromatographic imaging, comprise by optical fiber collimator (1), balzed grating, (2), fourier transform lens (3), galvanometer (4) and plane mirror (5), it is characterized in that: between optical fiber collimator (1) and original balzed grating, (2), increased another balzed grating, (6) newly, this balzed grating, (6) and original balzed grating, 2) parallel placement, the grating face of parallel and two balzed grating,s of two balzed grating, grooves is perpendicular to the optical axis of fourier transform lens; Perhaps the optical axis with fourier transform lens is not vertical for the grating face of two balzed grating,s; Perhaps the grating face of two balzed grating,s is not parallel; Perhaps the groove direction of two balzed grating,s is not parallel; The directional light that is penetrated by fiber optic collimator mirror (1) at first arrives the balzed grating, (6) that increases newly, diffraction light arrives original balzed grating, (2) and again by its diffraction behind its diffraction, the diffraction light of each wavelength focuses on the galvanometer (4) by fourier transform lens (3), the light of each wavelength reflects through galvanometer (4), after passing through fourier transform lens (3) again, successively by balzed grating, (2) and balzed grating, (6) diffraction, the light of various different wave lengths all will be parallel with former incident illumination this moment, the light of each wavelength incides on the plane mirror (5) of a reflecting surface and the vertical placement of each wavelength light direct of travel, former then road is returned, the light of each wavelength is final to be overlapped to a branch of, enters fiber optic collimator mirror (1).
6. the Dispersion Compensation Systems in the optical coherent chromatographic imaging according to claim 5, it is characterized in that: when the grating face of two balzed grating,s is not vertical with the optical axis of fourier transform lens (3), this system will introduce dynamic dispersion, the size of the dynamic dispersion of introducing is by the angle decision of grating face normal and optical axis, angle is big more, and it is big more to introduce dynamic dispersion.
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