CN1887074A - Method of measuring hatching rate, slush rate and death rate of brine shrimp simultaneously - Google Patents
Method of measuring hatching rate, slush rate and death rate of brine shrimp simultaneously Download PDFInfo
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- CN1887074A CN1887074A CN 200610107934 CN200610107934A CN1887074A CN 1887074 A CN1887074 A CN 1887074A CN 200610107934 CN200610107934 CN 200610107934 CN 200610107934 A CN200610107934 A CN 200610107934A CN 1887074 A CN1887074 A CN 1887074A
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Abstract
The method of measuring hatching rate, ghost rate and death rate of brine shrimp simultaneously includes setting sea water soaked white filter paper inside transparent glass culture dish and setting brine shrimp eggs before covering the dish with cover; hatching inside biochemical incubator under controlled temperature and lighting conditions; counting after hatching the hatched eggs with orange larva beside the egg, the death eggs with complete egg grain and the ghost egg with deformation and without larva beside the egg; and calculating the hatching rate, ghost rate and death rate of brine shrimp. The method is simple, convenient and accurate.
Description
Technical field
The present invention relates to a kind of detection artemia cysts method for quality, specifically relate to a kind of method of measuring artemia ovum hatching rate, ghost rate and dead ovum rate simultaneously.
Background technology
Along with the extensive utilization of artemia cysts in fields such as culture fishery and biological studies, its quality problems are more and more paid attention to by people, and wherein incubation rate, dead ovum rate and ghost rate are the important indicators of artemia cysts quality good or not.Present assay method is as follows:
(1), the artemia ovum hatching rate is measured: adopt several methods: count the artemia cysts of some before the hatching, count the nauplius number of hatching after the hatching again, thereby calculate its incubation rate; Or employing densimetry: be for the first time that the artemia cysts sample that soaks through 2h before hatching is stirred evenly quantitative sampling, meter artemia cysts number is for the second time to the sample quantitative sampling after the hatching, meter nauplius number, thus calculate its incubation rate; Or employing gravimetric method: take by weighing the artemia cysts sample of constant weight, hatching back separated and collected nauplius carries out weighing, rule of thumb its incubation rate of data computation.It is that artemia cysts is put into certain water body (about salinity 20) that operation during artemia ovum hatching requires, and is hatching 18h-40h under certain intensity of illumination under the water temperature condition about 28 ℃.
(2), the mensuration of artemia cysts ghost rate: take by weighing the artemia cysts sample of constant weight, in fresh water, behind the immersion 2h, the artemia cysts that floats on the water surface is collected, weigh after the oven dry, calculate the ghost rate.
(3), the mensuration of the dead ovum rate of artemia cysts: the dead ovum rate of artemia cysts can be measured simultaneously with incubation rate, method is that the artemia cysts sample of getting some is hatched, get a certain amount of equally distributed sample after the hatching and carry out molten shell (using clorox to carry out molten shell usually), nauplius number that obtains hatching and the unhatched ovum number that shells, thus calculate incubation rate and dead ovum rate.This method is not considered the existence of artemia cysts ghost rate in computational process, the result of mensuration is higher.
The said determination method once can only be measured 1 to 2 index, and when the incubation rate of measuring artemia cysts, ghost rate and dead ovum rate, operation also bothers.
Summary of the invention
The objective of the invention is deficiency, a kind of short-cut method that can measure artemia ovum hatching rate, ghost rate and dead ovum rate simultaneously is provided at existing assay method.
Determination techniques method of the present invention is:
1, put into the white filter paper that usefulness is wet from sea water in clear glass culture dish (diameter is advisable with 9-15cm), the filter paper size requirements can be paved with whole culture dish, and then adds the seawater of 1-2 milliliter, and filter paper is fully soaked.
2, put into 15-60 artemia cysts in above-mentioned culture dish, the ovum spacing remains on more than the 0.4mm, covers the culture dish lid.Put into 25-30 ℃ biochemical incubator then, more than the control intensity of illumination 1000lux, hatching 25-30h.
3, hatching finishes back taking-up culture dish, perusal (can use magnifying glass in case of necessity) counting.Every have a salmon pink artemia nauplii on artemia cysts next door, represent that this artemia cysts is the ovum that can normally hatch, and note is made n
1, n
1Also represent the artemia nauplii number that hatches simultaneously; Every do not have the salmon pink artemia nauplii on the artemia cysts next door, and ovum grain complete (artemia cysts does not have crackle or fragmentation), represent that this artemia cysts is dead ovum, note is made n
2Every do not have the salmon pink artemia nauplii on artemia cysts next door, and the ovum grain has crackle or broken, represents that this artemia cysts is the ghost ovum, and note is made n
3
4, calculate: incubation rate=n
1/ (n
1+ n
2+ n
3);
Dead ovum rate=n
2/ (n
1+ n
2+ n
3);
Ghost rate=n
3/ (n
1+ n
2+ n
3).
Characteristics of the present invention are:
1, utilizes the filter paper that fully soaks to provide artemia ovum hatching needed moisture, can avoid a large amount of of moisture to scatter and disappear by covering the culture dish lid simultaneously.
2, nauplius immotile substantially characteristics on filter paper of utilizing artemia ovum hatching to go out can accurate counting and can judge the young by which artemia ovum hatching is gone out.
3, owing to caving in the surface of finished product artemia cysts, and artemia cysts particle very little (general 1g artemia cysts has 160,000-300,000), therefore very difficult resolution ghost ovum, and the employing said method because the round change of artemia cysts suction change is big, is then differentiated than being easier to.
4, this method is simple to operate, application is convenient, the accuracy rate height.
Specific embodiments
The embodiment that is determined as that carries out with the artemia cysts of in the Huanghai Sea aquatic products research institute laboratory Huanghai Sea industry development parent company being produced in May, 2000 is described further below.
1, in 5 diameters are 9cm clear glass culture dish, puts into the white filter paper (filter paper diameter 11cm) that usefulness is wet from sea water respectively, add 2 milliliters seawater then in each culture dish again, filter paper is fully soaked.
2, put into about 30-40 artemia cysts respectively in above-mentioned 5 culture dishes, the ovum spacing remains on more than the 0.5mm, covers the culture dish lid.Put into 28 ℃ biochemical incubator then, control intensity of illumination 1500-2000lux, hatching 28h.
3, hatching finishes back taking-up culture dish, perusal counting.Result such as following table:
| The culture dish numbering | A | B | C | D | E | On average |
| Artemia cysts sum (individual) | 32 | 40 | 31 | 35 | 33 | |
| The nauplius number (individual) that the ovum number that can normally hatch promptly hatches | 21 | 27 | 22 | 26 | 24 | |
| Dead ovum number (individual) | 4 | 5 | 3 | 4 | 3 | |
| Ghost ovum number (individual) | 7 | 8 | 6 | 5 | 6 | |
| Incubation rate (%) | 65.6 | 67.5 | 71.0 | 74.3 | 72.7 | 70.2 |
| Dead ovum rate (%) | 12.5 | 12.5 | 9.7 | 11.4 | 9.1 | 11.1 |
| Ghost rate (%) | 21.9 | 20 | 19.3 | 14.3 | 18.2 | 18.7 |
Claims (1)
1, a kind of method of measuring artemia ovum hatching rate, ghost rate and dead ovum rate simultaneously is characterized in that its method is:
1), in the clear glass culture dish, put into the white filter paper that usefulness is wet from sea water, the filter paper size requirements can be paved with whole culture dish, and then adds the seawater of 1-2 milliliter, and filter paper is fully soaked;
2), in above-mentioned culture dish, put into 15-60 artemia cysts, the ovum spacing remains on more than the 0.4mm, covers culture dish lid; Put into 25-30 ℃ biochemical incubator then, more than the control intensity of illumination 1000lux, hatching 25-30h;
3), hatching finishes the back and take out culture dish, naked eyes or use magnifying glass to observe counting: every have a salmon pink artemia nauplii on the artemia cysts next door, represent that this artemia cysts is the ovum that can normally hatch, and note is made n
1, n
1Also represent the artemia nauplii number that hatches simultaneously; Every do not have the salmon pink artemia nauplii on artemia cysts next door, and the ovum grain complete be that artemia cysts does not have crackle or broken, represent that this artemia cysts is dead ovum, note is made n
2Every do not have the salmon pink artemia nauplii on artemia cysts next door, and the ovum grain has crackle or broken, represents that this artemia cysts is the ghost ovum, and note is made n
3
4), calculate: incubation rate=n
1/ (n
1+ n
2+ n
3); Dead ovum rate=n
2/ (n
1+ n
2+ n
3); Ghost rate=n
3/ (n
1+ n
2+ n
3).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006101079340A CN100397984C (en) | 2006-07-27 | 2006-07-27 | Method of measuring hatching rate, slush rate and death rate of brine shrimp simultaneously |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006101079340A CN100397984C (en) | 2006-07-27 | 2006-07-27 | Method of measuring hatching rate, slush rate and death rate of brine shrimp simultaneously |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1887074A true CN1887074A (en) | 2007-01-03 |
| CN100397984C CN100397984C (en) | 2008-07-02 |
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|---|---|---|---|
| CNB2006101079340A Expired - Fee Related CN100397984C (en) | 2006-07-27 | 2006-07-27 | Method of measuring hatching rate, slush rate and death rate of brine shrimp simultaneously |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103598151A (en) * | 2013-11-28 | 2014-02-26 | 天津海友佳音生物科技股份有限公司 | Artemia incubation device and method for incubating artemia with same |
| CN104488828A (en) * | 2014-12-18 | 2015-04-08 | 天津海友佳音生物科技股份有限公司 | Method for increasing hatching rate of artemia cysts after shelling |
| CN105203702A (en) * | 2015-08-28 | 2015-12-30 | 天津海友佳音生物科技股份有限公司 | Method for measuring proportion of pure matter in artemia cyst |
| CN109618999A (en) * | 2018-12-04 | 2019-04-16 | 天津海友佳音生物科技股份有限公司 | Separating liquid and its separation method after a kind of artemia ovum hatching with shell |
-
2006
- 2006-07-27 CN CNB2006101079340A patent/CN100397984C/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103598151A (en) * | 2013-11-28 | 2014-02-26 | 天津海友佳音生物科技股份有限公司 | Artemia incubation device and method for incubating artemia with same |
| CN104488828A (en) * | 2014-12-18 | 2015-04-08 | 天津海友佳音生物科技股份有限公司 | Method for increasing hatching rate of artemia cysts after shelling |
| CN105203702A (en) * | 2015-08-28 | 2015-12-30 | 天津海友佳音生物科技股份有限公司 | Method for measuring proportion of pure matter in artemia cyst |
| CN109618999A (en) * | 2018-12-04 | 2019-04-16 | 天津海友佳音生物科技股份有限公司 | Separating liquid and its separation method after a kind of artemia ovum hatching with shell |
Also Published As
| Publication number | Publication date |
|---|---|
| CN100397984C (en) | 2008-07-02 |
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Granted publication date: 20080702 |