CN1877307A - One-dimensional multi-detection up-converting phosphor biosensor - Google Patents
One-dimensional multi-detection up-converting phosphor biosensor Download PDFInfo
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- CN1877307A CN1877307A CN 200610027354 CN200610027354A CN1877307A CN 1877307 A CN1877307 A CN 1877307A CN 200610027354 CN200610027354 CN 200610027354 CN 200610027354 A CN200610027354 A CN 200610027354A CN 1877307 A CN1877307 A CN 1877307A
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Abstract
Disclosed is a phosphorescence biosensor which comprises an optical system, a photodetector, a data collect system, a control and display system, a driving module, a stepper motor and a scanning table for carrying test paper with multiple test zones. The optical system includes an infrared semi-conductor laser, a laser exciting light path, and a phosphorescence receiving light path, wherein the phosphorescence receiving light path is connected with the photodetector; and the output terminal of the photodetectror is connected with the data collect system which includes a first monolithic computer; the control and display system has a second monolithic computer; the first and the second monolithic computers can work at the same time.
Description
Technical field
The present invention relates to the up-converting phosphor biological detection, particularly a kind of one-dimensional multi-detection up-converting phosphor biosensor, this sensor can carry out interpretation as a result to the multiple detection immuno-chromatographic test paper strip based on the up-converting phosphor technology, thereby realizes the detection by quantitative to plurality of target checking matter in the sample.
Background technology
The up-converting phosphor technology is based on the up-converting phosphor material and a kind of novel markings that grows up and the technology of application.Up-converting phosphor material (Up-Converting Phosphor, be called for short UCP) is a kind ofly can go up the inorganic complex of commentaries on classics to photon energy, and promptly UCP can absorb the infrared light of low-yield (long wavelength), but launches high-energy (short wavelength's) visible light.Be connected by covalent bond with bioactive molecule as if thing that the up-conversion luminescence particle is served as a mark, and make it in the process of chromatography, be fixed in the surface of solid phase carrier by immune response, just can be infrared ray excited following, by immuno-chromatographic test paper strip is carried out interpretation, obtain the attribute and the content of checking matter molecule, realize detection by quantitative target checking matter in the biological sample.
Formerly in the technology, detecting instrument carries out mechanical scanning to the test strips that is positioned on the scanning platform, and the phosphorescence that sends after the UCP particle is stimulated is collected by optical system, sends into computing machine again after photodetector changes electric signal into, carries out data processing then.Have only a quality control band and a detection band on the test strips, promptly once can only detect a kind of target checking matter in the sample.And, high trapping phenomena may occur for some reason in the test strips chromatography process and make this test strips defective, formerly technology is monitored its biological respinse state.Formerly in the technology, control and data acquisition and processing by same computing machine, can only sequential operation, can not parallel processing, caused waste of time; And the power consumption of computing machine is bigger, can not adopt dry cell power supply.Keyboard, mouse are external unit, and be not portable.
The shortcoming of above-mentioned technology formerly is:
1, detection efficiency is low.Because have only a quality control band and a detection band on the test strips, so once can only detect a kind of target checking matter in the sample.If detect a plurality of target checking matter in the sample, then must use a plurality of test strips, thereby detection speed is slow, efficient is low, and required sample size is big.
2, lack the monitoring value in the interpretation process, can't judge whether biological respinse is normal.Owing to making this detection unsuccessful because high trapping phenomena may appear in some reason in the test strips chromatography process,, then may do the judgement that makes mistake to this test result of samples if its biological respinse state is not monitored.
3, time and power wastage.Control and data acquisition and processing by same computing machine, can only sequential operation, can not parallel processing, caused waste of time.
4, can not work alone under the lowered in field environment.Because power consumption is big, can not adopt dry cell power supply, and keyboard, mouse be external unit, not portable, cause instrument not work alone under the lowered in field environment.
Summary of the invention
The objective of the invention is to overcome the problem of above-mentioned technology formerly, a kind of one-dimensional multi-detection up-converting phosphor biosensor is provided, this sensor should have following advantage: can accurately measure a quality control band and an a plurality of detection band on the test strips and the amount that the UCP particle on the high zone of being detained may occur, both the state of biological respinse on the decidable test strips can be realized multiple detection again.Simultaneously, data acquisition system (DAS) is separated with control and display system, and the operation of two systems can be carried out simultaneously, has saved the time greatly.Adopt dry cell power supply, substitute the formerly external keyboard and the mouse of technology with simple and easy membrane keyboard, instrument can work alone under the lowered in field environment.
Be different from formerly said " the single detection " that once can only detect a kind of target checking matter in the sample in the technology, the present invention can detect i.e. the present invention said " multiple detection " simultaneously to two or more the target checking matter in the sample.
Technical solution of the present invention is as follows:
A kind of one-dimensional multi-detection up-converting phosphor biosensor is characterized in that forming by optical system, photodetector, data acquisition system (DAS), control and display system, driver module, stepper motor and for the scanning platform that the test strips with a plurality of detection bands is set:
Described optical system comprises infrared semiconductor laser, excitation light path, the phosphorescence receiving light path, the excitation beam that is sent by infrared semiconductor laser shines on described test strips by described excitation light path, described phosphorescence receiving light path connects described photodetector, the described data acquisition system (DAS) of output termination of described photodetector, this data acquisition system (DAS) has first single-chip microcomputer, described control and display system have second singlechip, first single-chip microcomputer and second singlechip can be worked respectively simultaneously, described data acquisition system (DAS) links to each other with described control and display system, and this data acquisition system (DAS) drives the motion of the described scanning platform of described step motor control again by driver module.
Described data acquisition system (DAS) is by first single-chip microcomputer, the AD chip, extend out RAM, extend out the I/O mouth, RS232 interface and address latch are formed, described first single-chip microcomputer connects described AD chip on the one hand respectively, extend out the RAM data terminal, extend out the I/O mouth, RS232 interface and address latch, and by address latch with extend out the address ram end and link to each other, also link to each other on the other hand with described control and display system, described scanning platform links to each other with described stepper motor, described stepper motor links to each other with first single-chip microcomputer by driver module again, and described photodetector output terminal links to each other with first single-chip microcomputer by described AD chip again.
Described control and display system by second singlechip, LCDs (hereinafter to be referred as LCD), clock chip, membrane keyboard, extend out RAM, extend out the I/O mouth, RS232 interface, printer and address latch form, described second singlechip on the one hand respectively with LCD, clock chip, membrane keyboard, extend out RAM, extend out the I/O mouth, RS232 interface, printer links to each other with address latch, and by described address latch with extend out the address ram end and link to each other, also link to each other on the other hand with first single-chip microcomputer of described data acquisition system (DAS).
Described test strips has the well except that having, and also has quality control band, a plurality of detection band and a guarded region.
Described optical system is made up of excitation source, excitation light path and phosphorescence receiving light path.Described excitation source is an infrared semiconductor laser.
Described photodetector is photomultiplier (PMT), the described data acquisition system (DAS) of its output termination, and this data acquisition system (DAS) links to each other with described control and display system.
The course of work of the present invention is as follows:
The excitation beam that described infrared semiconductor laser sends is through forming a rectangular light spots on the test strips surface behind the excitation light path, and the length direction of rectangular light spots is consistent with the Width of test strips.A quality control band and a plurality of detection band on the rectangular light spots scanning test strips inspire visible phosphorescence.Collect and be converted to electric signal by described photomultiplier behind the phosphorescent signal process phosphorescence receiving light path.Electric signal after the conversion is sent into data acquisition system (DAS) analysis.
After first single-chip microcomputer of described data acquisition system (DAS) obtains " beginning " order that the second singlechip of described control and display system sends over, by its I/O mouth software programming is produced the step motor drive pulse, the first microcomputer monitoring stepper motor is changed by the data after the PMT conversion with instant driving AD chip simultaneously.The stepper motor sampled point distance of advancing, the AD chip is just once changed, until the test strips been scanned.The data that data acquisition system (DAS) collects are by extending out RAM storage, and output in control and the display system and analyze and handle by extending out IO in real time.After once collection finished, scanning platform resetted.At the same time, described control and display system are sent the LCD screen curve map that draws the data acquisition system (DAS) that receives by the acquired original data that extend out I/O and send into, the position of quality control band, each detection band and guarded region is determined in manual intervention, calculates net result.Also can set detected parameters next time simultaneously.
The second singlechip of described control and display system is accepted user instruction by continuous keyboard scan and is changed corresponding subroutine over to and handle.Time was sent into second singlechip every 1 minute by external interrupt by clock chip.Operation steps of user, input data and testing result etc. are all shown by the LCD screen.
Compare with technology formerly, technique effect of the present invention is as follows:
1, detection efficiency height.A quality control band and a plurality of detection band are arranged on the test strips, promptly once can detect the plurality of target checking matter in a kind of sample.Improved detection efficiency, and required sample size is few.
2, can calculate the phosphorescent signal Q of easy retention areas, and set the monitoring value, judge whether biological respinse is normal.The present invention can select easy retention areas position and calculate the Qc value, compares with the monitoring value that sets, if greater than this value, then the biological respinse on the test strips is undesired, and this detection is invalid; If less than this value, then proceed the test strips interpretation, provide net result.Thereby can avoid the error-detecting result's that causes because of biological respinse is undesired generation.
3, detection speed is fast.Adopt two Single Chip Microcomputer (SCM) system, be that data acquisition system (DAS) is separated with control and display system, the operation of two systems can be carried out simultaneously, in data acquisition system gated sweep platform reseting procedure, the result of calculation output simultaneously of control and display system, parameters such as sample ID number, decision content are set, have saved detection time greatly.
But work alone under 4 lowered in field environment.The present invention adopts Single Chip Microcomputer (SCM) system, and is low in energy consumption, can be by dry cell power supply, and substitute external keyboard and mouse in the technology formerly with simple and easy membrane keyboard, can work independently.
5, Single Chip Microcomputer (SCM) system of the present invention can realize the demonstration of complex operations interface and arbitrary graphic on high resolving power full screen graphical dots configuration LCD.
Description of drawings
Fig. 1 is the structured flowchart of one-dimensional multi-detection up-converting phosphor biosensor of the present invention.
Fig. 2 is the structural representation of the test strips that adopts of the present invention.
Fig. 3 is an optical system structure block diagram of the present invention.
Fig. 4 is a data acquisition system (DAS) structured flowchart of the present invention.
Fig. 5 is control of the present invention and display system architectures block diagram.
Fig. 6 is a control flow chart of the present invention.
Fig. 7 is a data processing algorithm process flow diagram of the present invention.
Fig. 8 is the data and curves figure of most preferred embodiment of the present invention.
Embodiment
The invention will be further described below in conjunction with drawings and Examples, but should not limit protection scope of the present invention with this.
See also Fig. 1, Fig. 1 is the structured flowchart of one-dimensional multi-detection up-converting phosphor biosensor of the present invention.Also be one embodiment of the present of invention, as seen from the figure, embodiment of the invention one-dimensional multi-detection up-converting phosphor biosensor has a plurality of detections and forms with the scanning platform 7 of 803,804 test strips 8 by optical system 1, photodetector 2, data acquisition system (DAS) 3, control and display system 4, driver module 5, stepper motor 6 and for being provided with:
Described optical system 1 comprises excitation source 101, excitation light path 102, phosphorescence receiving light path 103, see Fig. 3, the excitation beam that is sent by excitation source 101 shines on described test strips 8 by excitation light path 102, described phosphorescence receiving light path 103 connects described photodetector 2, the described data acquisition system (DAS) 3 of the output termination of described photodetector 2, this data acquisition system (DAS) 3 has first single-chip microcomputer 301, described control and display system 4 have second singlechip 401, described data acquisition system (DAS) 3 first single-chip microcomputers 301 link to each other with described control and display system 4 second singlechips 401, and this data acquisition system (DAS) 3 drives the motion of the described scanning platform 7 of described stepper motor 6 controls again by driver module 5.
Described data acquisition system (DAS) 3, referring to Fig. 4, by first single-chip microcomputer 301, AD chip 302, extend out RAM303, extend out I/O mouth 304, RS232 interface 305 and address latch 306 are formed, described first single-chip microcomputer 301 connects described AD chip 302 on the one hand respectively, extend out the RAM303 data terminal, extend out I/O mouth 304, RS232 interface 305 and address latch 306, and by address latch 306 with extend out RAM303 address end and link to each other, also link to each other on the other hand with described control and display system 4, described scanning platform 7 links to each other with described stepper motor 6, described stepper motor 6 links to each other with first single-chip microcomputer 301 by driver module 5 again, and described photodetector 2 output terminals link to each other with first single-chip microcomputer 301 by described AD chip 302 again.
Described control and display system 4, referring to Fig. 5, by second singlechip 401, LCD402, clock chip 403, membrane keyboard 404, extend out RAM405, extend out I/O mouth 406, RS232 interface 407, printer 408 and address latch 409 are formed, described second singlechip 401 on the one hand respectively with LCD402, clock chip 403, membrane keyboard 404, extend out RAM405, extend out I/O mouth 406, RS232 interface 407, printer 408 links to each other with address latch 409, and by address latch 409 with extend out RAM405 address end and link to each other, also link to each other on the other hand with first single-chip microcomputer 301 of described data acquisition system (DAS) 3.
Described test strips 8 sees also Fig. 2, and it also has a quality control band 805, first to detect and be with 803, second detection to be with 804 and guarded regions 802 except that a well 801 is arranged.
Described excitation source 101 is an infrared semiconductor laser.
Described photodetector 2 is a photomultiplier.
The course of work of the present invention is as follows:
Test strips to be detected 8 is positioned on the scanning platform 7.The excitation beam that infrared semiconductor laser 101 sends forms a rectangular light spots through excitation light path 102 on test strips 8 surfaces, the length of rectangular light spots and width should be complementary with test strips 8 width and scanning resolution of the present invention respectively, have advantages of higher stability and sensitivity to guarantee the present invention.And by 7 motions of data acquisition system (DAS) 3 control and driving module 5 drive stepping motor 6 drive scanning platforms, make rectangular light spots scan whole test strips 8, comprising that quality control band 805, first detects is with 803, second to detect and be with a plurality of detection bands such as 804, and guarded region 802, sees also Fig. 2.At this moment, be deposited on after UCP particle on test strips 8 each function band is subjected to infrared laser and excites, send visible phosphorescence by last conversion.Phosphorescent signal intensity on each function band is exactly the signal that the present invention will detect.This phosphorescent signal is converted to electric signal through phosphorescence receiving light path 103 by described photodetector 2 again.
See also Fig. 4, after first single-chip microcomputer 301 receives the collection initiation command that is sent by control and display system 4, just gather the signal of photodetector 2 outputs, and drive scanning platforms 7 by driver module 5 drive stepping motor 6 and move, simultaneously it is monitored in real time, the simulating signal of photodetector 2 outputs enters the analog input end of AD chip 302, this moment, first single-chip microcomputer, 301 driving AD chips 302 carried out analog to digital conversion, and the result that will change sends into and extends out RAM 303, and address latch 306 is in order to latch selected address.After gather finishing, notice control and display system 4 data acquisitions finish, wait to be controlled and display system 4 is obtained data.Can carry out communication with the PC computing machine by RS232 interface 305, the first single-chip microcomputers 301.
See also Fig. 5, after system powers on, second singlechip 401 is just controlled LCD and is shielded the prompting of 402 explicit users, wait for user input commands by keyboard 404, the user can modification time, setting operation person and is detected thing, sets sample ID number, sets decision content and monitoring value, sends the beginning sense command, checks and print instruction such as examining report.Second singlechip 401 just drives corresponding peripheral chip work after receiving corresponding instruction.The modification of time is read and write to clock chip 403 by second singlechip 401 and is finished, and the renewal of time is sent into second singlechip 401 by clock chip 403 by external interrupt.After the user finished the parameter setting and sends the beginning sense command, second singlechip 401 just sent commencing signal to data acquisition system (DAS) 3, waits the signal that finishes to be collected subsequently.After collection finishes, second singlechip 401 is by extending out the data that I/O mouth 406 reading of data acquisition systems 3 are sent, and send LCD screen 402 curve map that draws, the position of quality control band and a plurality of detection bands is determined in manual intervention, calculate the result, make the result and judge, and show examining report by LCD screen 402, this moment, but user's selective printing was exported the result by printer 408.
Fig. 6 is a control flow chart of the present invention.Utilize membrane keyboard 404 setting operation persons earlier, detect each parameters such as thing, sample ID, decision content and monitoring value, send " beginning " instruction by user key-press then, data acquisition system (DAS) 3 begins to gather the signal of photodetector 2 outputs, and drive scanning platforms 7 by data acquisition system (DAS) 3 control and driving module 5 drive stepping motor 6 and move, simultaneously it is monitored in real time, after collection finished, scanning platform 7 resetted, and stopped to detect.The electric signal of photodetector 2 outputs enters AD chip 302 analog end, is converted to digital signal, and data deposit external RAM in.Wait for the new detection of instruction beginning next time.
Fig. 7 is flow chart of data processing figure of the present invention.Second singlechip 401 reads the data of external RAM, deliver to LCD 402 curve map that draws, automatically search for quality control band and a plurality of detections region earlier, manually determine quality control band then and detect the band position, be the peak value place of move left and right index line to each band, see also Fig. 8, wherein Q is the signal of guarded region, C is the signal of quality control band 805, T
1, T
2Be respectively first and detect the signal that detects with 804 with 803 and second, if test strips includes more than two detection bands, then each signal that detects band is followed successively by T
1, T
2, T
3T
n(n＞=2).In the chromatography process, be added to sample spot on the test strips 8 by well 801 after, fluid sample detects through guarded region 802, first successively and is with 803, second to detect and be with 804 and quality control band 805 under the capillary syphonic effect of adsorptive pads.This process can cause certain sample deposition, the corresponding noise data of this deposition roughly can fit to a slope near each function band be positive straight line, therefore want earlier the data sementation that collects is deducted the value of the different straight line of several slopes, promptly remove background noise.Then, obtain around the center position of quality control band and nearest with it detection band 5 mean value, the signal Q of guarded region then obtains the Qc value divided by mean value.Qc value and the monitoring value of setting are made comparisons, if " the Qc value〉the monitoring value ", then test strips is made mistakes; As if " Qc value＜monitoring value ", then continue to calculate the signal C of quality control band 805, first detects the signal T that detects with 804 with 803 and second
1, T
2, promptly respectively the signal of a plurality of points around the peak of each band is averaged as the signal value of this function band, and then calculates their ratio T
1/ C and T
2/ C, these ratios are exactly the result that the present invention finally will obtain, and the checking matter concentration that contains in this result and the sample is linear, promptly according to the different proportional or inverse relation of reaction pattern.
Be the concrete device and the structural parameters of the embodiment of the invention below: infrared semiconductor laser 1 is that peak wavelength is the semiconductor laser of 980nm, and output power is 80mW.Excitation beam forms a rectangular light spots through excitation light path 102 on test strips 8 surfaces, and the length of rectangular light spots is 2.5mm, and width is 30 μ m.The peak wavelength of the phosphorescence that is inspired is 541.5nm.Photodetector 2 is PMT, and photosurface is of a size of φ 8mm, and wavelength response range is 300-850nm, is that the exciting light of 980nm does not have response to wavelength, portion is integrated within it prime amplifier, and its output voltage range is 0-10V.Totally 250 of sampled points, sampling resolution are 32 μ m.What first single-chip microcomputer 301 and second singlechip 401 were selected for use is the 89V51RD type of Philips, and it has the internal RAM of 1kbit and the inside ROM of 64kbit, can fully satisfy system's needs.AD chip 302 has 12 precision, and full scale is 10V, and precision can reach 2.44mV.LCD screen 402 is a full screen graphical dots configuration liquid crystal display assembly, is made up of controller, driver and full lattice lcd display.
Fig. 8 is that most preferred embodiment of the present invention detects the data and curves figure that obtains to a test strips that can detect two kinds of target checking matters in the sample, and this test strips can detect Bacillus anthracis and plague bacillus.Wherein, T
1For combining first phosphorescent signal that detects with 803 of Bacillus anthracis, T in the immune response
2For combining second phosphorescent signal that detects with 804 of plague bacillus in the immune response, C is the signal of quality control band 805.Because the function band itself has the width of 1mm, corresponding to a plurality of sampled points, the present invention averages as the signal value of this function band to the signal of a plurality of points around the peak respectively, calculates thus and can get T
1=0.85889V, T
2=4.75300V, C=4.33823V.Obtaining first at last detects with 803 and second detection with the 804 ratio T with respect to the quality control band signal
1/ C=0.19788, T
2/ C=1.09561 compares the decision content of these two ratios and user input just and can make interpretation as a result.
Present embodiment is the rechargeable cell power supply of 12V with voltage, and present embodiment is moved more than 4 hours continuously, therefore can work alone under the lowered in field environment.
Shows that through on probation the present invention can accurately measure a quality control band and an a plurality of detection band on the test strips and the amount that the UCP particle on the high zone of being detained may occur, both the state of biological respinse on the decidable test strips can be realized multiple detection again.Simultaneously, data acquisition system (DAS) is separated with control and display system, and the operation of two systems can be carried out simultaneously, has saved the time greatly.Adopt dry cell power supply, substitute the formerly external keyboard and the mouse of technology with simple and easy membrane keyboard, instrument can work alone under the lowered in field environment.
Claims (6)
1, a kind of one-dimensional multi-detection up-converting phosphor biosensor is characterized in that by optical system (1), photodetector (2), data acquisition system (DAS) (3), control and display system (4), driver module (5), stepper motor (6) and supplies to place the scanning platform (7) with a plurality of detection bands (803,804) test strips (8) forming:
Described optical system (1) comprises excitation source (101), excitation light path (102), phosphorescence receiving light path (103), the excitation beam that is sent by excitation source (101) shines on described test strips (8) by excitation light path (102), described phosphorescence receiving light path (103) connects described photodetector (2), the described data acquisition system (DAS) of output termination (3) of described photodetector (2), this data acquisition system (DAS) (3) has first single-chip microcomputer (301), described control and display system (4) have second singlechip (401), described data acquisition system (DAS) (3) first single-chip microcomputers (301) link to each other with described control and display system (4) second singlechip (401), and this data acquisition system (DAS) (3) drives the motion that described stepper motor (6) is controlled described scanning platform (7) by driver module (5) again.
2, one-dimensional multi-detection up-converting phosphor biosensor according to claim 1, it is characterized in that described data acquisition system (DAS) (3) is by first single-chip microcomputer (301), AD chip (302), extend out RAM (303), extend out I/O mouth (304), RS232 interface (305) and address latch (306) are formed, described first single-chip microcomputer (301) connects described AD chip (302) on the one hand respectively, extend out RAM (303) data terminal, extend out I/O mouth (304), RS232 interface (305) and address latch (306), and by address latch (306) with extend out RAM (303) address end and link to each other, also link to each other on the other hand with described control and display system (4), described scanning platform (7) links to each other with described stepper motor (6), described stepper motor (6) links to each other with first single-chip microcomputer (301) by driver module (5) again, and described photodetector (2) output terminal links to each other with first single-chip microcomputer (301) by described AD chip (302) again.
3, one-dimensional multi-detection up-converting phosphor biosensor according to claim 1, it is characterized in that described control and display system (4) are by second singlechip (401), LCDs (402), clock chip (403), membrane keyboard (404), extend out RAM (405), extend out I/O mouth (406), RS232 interface (407), printer (408) and address latch (409) are formed, described second singlechip (401) on the one hand respectively with LCD (402), clock chip (403), membrane keyboard (404), extend out RAM (405), extend out I/O mouth (406), RS232 interface (407), printer (408) links to each other with address latch (409), and by address latch (406) with extend out RAM (405) address end and link to each other, also link to each other on the other hand with first single-chip microcomputer (301) of described data acquisition system (DAS) (3).
4, one-dimensional multi-detection up-converting phosphor biosensor according to claim 1, it is characterized in that described test strips (8) and have the well (801) to also have a quality control band (805), a plurality of detection band (803,804) and a guarded region (802) except that having.
5, one-dimensional multi-detection up-converting phosphor biosensor according to claim 1 is characterized in that described excitation source (101) is an infrared semiconductor laser.
6,, it is characterized in that described photodetector (2) is a photomultiplier according to the described one-dimensional multi-detection up-converting phosphor biosensor of claim 1 to 5.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104111330A (en) * | 2014-07-18 | 2014-10-22 | 中国科学院上海光学精密机械研究所 | Up-conversion luminescence immunochromatography analyzer and detection method thereof |
| CN104345048A (en) * | 2013-08-07 | 2015-02-11 | 杨晓林 | Method for detecting biological molecules based on upconversion luminescent material and detection system implementing same |
| CN105628912A (en) * | 2014-11-03 | 2016-06-01 | 天津市普瑞仪器有限公司 | Immune chromatographic analyzer |
| CN107037206A (en) * | 2017-03-31 | 2017-08-11 | 深圳市在田翊方科技有限公司 | A kind of time-resolved fluoroimmunoassay chromatography |
| CN110568179A (en) * | 2019-11-11 | 2019-12-13 | 上海奥普生物医药有限公司 | device and method for realizing multi-line extensible detection of immunochromatography analyzer |
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2006
- 2006-06-07 CN CN 200610027354 patent/CN1877307A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104345048A (en) * | 2013-08-07 | 2015-02-11 | 杨晓林 | Method for detecting biological molecules based on upconversion luminescent material and detection system implementing same |
| CN104345048B (en) * | 2013-08-07 | 2016-12-07 | 杨晓林 | A kind of based on the up-conversion luminescence material detection method of biomolecule and the detecting system implementing it |
| CN104111330A (en) * | 2014-07-18 | 2014-10-22 | 中国科学院上海光学精密机械研究所 | Up-conversion luminescence immunochromatography analyzer and detection method thereof |
| CN104111330B (en) * | 2014-07-18 | 2016-03-09 | 中国科学院上海光学精密机械研究所 | Up-conversion luminescence immunochromatographiassays assays instrument and detection method |
| CN105628912A (en) * | 2014-11-03 | 2016-06-01 | 天津市普瑞仪器有限公司 | Immune chromatographic analyzer |
| CN107037206A (en) * | 2017-03-31 | 2017-08-11 | 深圳市在田翊方科技有限公司 | A kind of time-resolved fluoroimmunoassay chromatography |
| CN107037206B (en) * | 2017-03-31 | 2019-03-05 | 深圳市在田翊方科技有限公司 | A kind of time-resolved fluoroimmunoassay chromatography |
| CN110568179A (en) * | 2019-11-11 | 2019-12-13 | 上海奥普生物医药有限公司 | device and method for realizing multi-line extensible detection of immunochromatography analyzer |
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