CN1876139A - Medicinal preparation for treating children's fastidium, its preparation process and quality control method - Google Patents

Abstract

The invention relates to a pharmaceutical preparation for treating children's apocleisis, its preparing process and quality control method, wherein the preparation is made mainly from medicated leaven, bitter orange, white atractylodes rhizome and Chinese haw.

Description

Pharmaceutical preparation of treatment infantile anorexia and preparation method thereof and method of quality control

Technical field: the present invention is a kind of pharmaceutical preparation for the treatment of infantile anorexia and preparation method thereof and method of quality control, belongs to technical field of Chinese medicine.

Technical background: anorexia, calcium deficiency are the common diseases of children's.Apositia is that the long appetite of children's is low, is subjected to the obviously symptom of influence to cause the normal nutritional development of children's.Infantile anorexia mainly is because the digestive functions disorder causes due to loss of appetite or the disappearance.Common cause has improper feeding, and living environment changes, psychentonia, drug influence, sickness influence etc.The anorexia overlong time can cause infant malnutrition, weak, the resistance decline of health, and so just easier generation other diseases causes adverse consequences.Infant lacks calcareous meeting and causes rickets, and it mainly shows is the skeleton hypocalcification, and hardness is relatively poor, thereby occurs that rib turns up, pigeon chest, funnel chest, and thorax and lower limb malformation such as bowlegs, can influence children's's growth promoter.The rickets prevalence is up to 40.7% in the infant, by Ministry of Public Health classify as the department of pediatrics keypoint control four big diseases it.Chinese medicine infantile anorexia, calcium deficiency etc. have one's own knack, wherein commercially available XIAOER XISHI PIAN, XIAOER XISHI TANGJIANG, XIAOER XISHI KELI are exactly curative effect Chinese medicine compound product preferably, but we discover that all there is same problem in these three kinds of products, that is exactly in order to improve mouthfeel, added a large amount of sucrose, this growth to the child is not favourable, and particularly some Obese children are unsuitable for taking especially; Along with people to improving constantly that quality of the life requires, the demand of Sugarless type product and novel form increases day by day; In view of such circumstances, in order to improve bioavailability of medicament, need a kind of therapeutic effect ideal of searching, steady quality, the reasonably effective medicine preparation of dosage form to enrich the dosage form kind, satisfy market demand.And, make said preparation reach satisfactory effect, and can standard production management, and it is done further research and more new development, at first said preparation will have the quality of stable and controllable; And that existing children's likes the method for quality control of food effervescent formulation is comparatively rough, is difficult to reach modern medicines requirement stable and controllable for quality; Therefore, need the research children's to like food effervescent formulation new Method of Quality.

Summary of the invention: the objective of the invention is to: a kind of happiness food pharmaceutical preparation for the treatment of infantile anorexia and preparation method thereof and method of quality control are provided; The preparation that provides comprise have that drug effect is rapid, bioavailability is high, effervescent tablet, oral liquid, the gel of characteristics such as easy to carry, good mouthfeel, be specially adapted to the patient that the child can not swallow solid preparation; The present invention also provides the method for preparation and the method for quality control, to instruct the production of enterprise, guarantees the effective of product.

The present invention constitutes like this: the pharmaceutical preparation of treatment infantile anorexia: it is by Massa Medicata Fermentata 385g, Fructus Aurantii 195g, Rhizoma Atractylodis Macrocephalae 195g, Fructus Crataegi 295g, Fructus Oryzae Germinatus 960g, Fructus Hordei Germinatus 960g or be made into effervescent tablet with their extract of corresponding weight portion, injection, the powder pin, freeze-dried powder, gel, dispersible tablet, capsule, soft capsule, microcapsule, granule, pill, micropill, powder, drop pill, slow releasing preparation, controlled release preparation, gel, oral liquid, soft extract, extractum or membrane.

Specifically: described preparation is effervescent tablet, oral liquid, gel or pellet.

The preparation method of pharmaceutical preparation of treatment infantile anorexia: get Massa Medicata Fermentata, Fructus Hordei Germinatus, Fructus Oryzae Germinatus and add behind the water boil, left standstill 4 hours, filter filtrate for later use 80～90 ℃ of dippings 2 hours; Other gets the Rhizoma Atractylodis Macrocephalae, Fructus Aurantii, Fructus Crataegi and decocts with water secondary, and each 1.5 hours, collecting decoction left standstill, filter, filtrate and above-mentioned filtrate merge, and are concentrated into relative density and are about 1.20 clear paste, drying under reduced pressure, pulverize, the cream powder that sieves is made different preparations then respectively.

Effervescent tablet in the described preparation prepares like this: get Massa Medicata Fermentata, Fructus Hordei Germinatus, Fructus Oryzae Germinatus and add behind the water boil 80～90 ℃ of dippings 2 hours, left standstill 4 hours, filter filtrate for later use; Other gets the Rhizoma Atractylodis Macrocephalae, Fructus Aurantii, Fructus Crataegi and decocts with water secondary, and each 1.5 hours, collecting decoction, leave standstill, filter, filtrate and above-mentioned filtrate merge, relative density is 1.20 clear paste when being concentrated into 50 ℃, drying under reduced pressure is pulverized the cream powder that sieves, the cream powder adds sodium bicarbonate 2500g, citric acid 600g, fumaric acid 300g, steviosin 10g, magnesium stearate 25g, lactose 550g, mix homogeneously, tabletting, pressure is at 4.0～5.0kg/cm ², promptly.

Gel in the described preparation prepares like this: get Massa Medicata Fermentata, Fructus Hordei Germinatus, Fructus Oryzae Germinatus and add behind the water boil 80～90 ℃ of dippings 2 hours, left standstill 4 hours, filter filtrate for later use; Other gets the Rhizoma Atractylodis Macrocephalae, Fructus Aurantii, Fructus Crataegi and decocts with water secondary, and each 1.5 hours, collecting decoction left standstill, and filters filtrate and the merging of above-mentioned filtrate; Konjac glucomannan, K-carrageenan are pressed 1: 3 part by weight mix homogeneously, under stirring condition, 0.8% mixed-matrix is put into cold water, make it to disperse, soak 20～30min, make the abundant water absorption and swelling of substrate, stir then, heat, filter, filter the back and under condition of stirring, add herbal extract, mix homogeneously joins rapidly in the disinfecting container, seals, sterilization is cooled to about 30 ℃ condensation rapidly, drying, promptly.

The method of quality control of the pharmaceutical preparation of treatment infantile anorexia: this method comprises following all or part of content:

(1) the differential test method of the Rhizoma Atractylodis Macrocephalae, Fructus Crataegi, ursolic acid, Fructus Hordei Germinatus, Fructus Aurantii, naringin, flavones ingredient;

(2) naringin, content of ursolic acid method of testing.

The discrimination method of described preparation comprises following all or part of content:

A. the thin layer chromatography discrimination method of the Rhizoma Atractylodis Macrocephalae in the preparation:

Getting children's, to like the food preparation an amount of, adds diethyl ether or chloroform or ethyl acetate jolting are extracted, and extracting solution volatilizes, and residue adds chloroform or ethyl acetate or methanol or ethanol makes dissolving, as need testing solution; Other gets Rhizoma Atractylodis Macrocephalae control medicinal material, decocts with water, and filters, and filtrate adds diethyl ether or chloroform or ethyl acetate jolting are extracted, and extracting solution volatilizes, and residue adds chloroform or ethyl acetate or methanol or ethanol makes dissolving, in contrast medical material solution; Draw each 1～30 μ l of above-mentioned two kinds of solution, put respectively in same silica gel G or silica gel H or silica gel G F ₂₅₄On the lamellae, with chloroform or dichloromethane-acetone or butanone-formic acid or acetic acid=1～10: be developing solvent at 0～2: 0～1, launches, and takes out, and dries, and puts under the ultra-violet lamp and inspect; In the test sample chromatograph, with the corresponding position of control medicinal material chromatograph on, show the fluorescence speckle of same color;

B. one or both thin layer chromatography discrimination method in Fructus Crataegi, the ursolic acid in the preparation:

Get children's like the food preparation an amount of, add diethyl ether or chloroform or ethyl acetate or ethanol or methanol or acetone extraction the extracting solution evaporate to dryness, residue soaks with petroleum ether, the petroleum ether that inclines, residue add methanol or chloroform or ethyl acetate or ethanol makes dissolving, as need testing solution; Other gets the Fructus Crataegi control medicinal material, decocts with water, and decocting liquid adds diethyl ether or chloroform or ethyl acetate extraction, and extracting solution volatilizes, and residue adds methanol or chloroform or ethyl acetate or ethanol makes dissolving, in contrast medical material solution; Get the ursolic acid reference substance again, add methanol or chloroform or ethyl acetate or ethanol and make dissolving, in contrast product solution; Draw one or both each 1～30 μ l in above-mentioned need testing solution and the contrast solution, put respectively in same silica gel G or silica gel H or silica gel G F ₂₅₄On the lamellae, with chloroform or dichloromethane-acetone or butanone=1～10: 0～1 is developing solvent, or with benzene or toluene-ethyl acetate or butyl acetate or Ethyl formate-or formic acid or acetic acid=1～20: be developing solvent at 1～4: 0～2, launch, take out, dry, spray is with ethanol solution of sulfuric acid, and it is clear to be heated to speckle colour developing; In the test sample chromatograph, with contrast chromatograph corresponding position on, show the speckle of same color;

C. the thin layer chromatography discrimination method of Fructus Hordei Germinatus in the preparation:

Get children's like the food preparation an amount of, add dehydrated alcohol or methanol extraction, extracting solution adds strong base solution, reflux, cooling rapidly, in the dislocation separatory funnel, it is an amount of to add water, with petroleum ether or ether extraction, extracting solution volatilizes, and residue adds ethyl acetate or chloroform makes dissolving, as need testing solution; Other gets the Fructus Hordei Germinatus control medicinal material, shines medical material solution in pairs with legal system; Draw each 1～30 μ l of above-mentioned two kinds of solution, put respectively in same silica gel G or silica gel H or silica gel G F ₂₅₄On the lamellae, with benzene or toluene or dimethylbenzene-chloroform or dichloromethane=0～1: 0～1 is developing solvent, launches, and takes out, and dries, and spray to be to contain the alcoholic solution of nitric acid, and it is clear to be heated to the speckle colour developing, puts under the ultra-violet lamp and inspects; In the test sample chromatograph, with the corresponding position of control medicinal material chromatograph on, show the fluorescence speckle of same color;

D. the thin layer chromatography discrimination method of Fructus Aurantii in the preparation:

Getting children's, to like the food preparation an amount of, adds ethanol or methanol or acetone extraction, and extracting solution concentrates, as need testing solution; Other gets the Fructus Aurantii control medicinal material, shines medical material solution in pairs with legal system; Draw each 1～30 μ l of above-mentioned two kinds of solution, put respectively in same silica gel G or silica gel H or silica gel G F ₂₅₄On the lamellae, with cyclohexane extraction or normal hexane-chloroform or dichloromethane-ethyl acetate or butyl acetate or Ethyl formate=5～20: be developing solvent at 1～5: 1～5, launches, and takes out, and dries, and puts under the ultra-violet lamp and inspect; In the test sample chromatograph, with the corresponding position of control medicinal material chromatograph on, show the fluorescence speckle of same color;

E. the high performance liquid chromatography discrimination method of naringin in the preparation:

It is an amount of that children's likes the food preparation, adds ethanol or methanol extraction, and extracting solution filters, as need testing solution; It is an amount of to get the naringin reference substance, adds ethanol or methanol is made reference substance solution; With octadecylsilane chemically bonded silica or eight alkyl silane bonded silica gels or dialkyl silane bonded silica gel is filler; Methanol or acetonitrile-water-acetic acid or formic acid or phosphoric acid=5～35: 15～65: 0～5 is mobile phase; The detection wavelength is one or several among 190～140nm, and number of theoretical plate calculates by the naringin peak should be not less than 1000; Draw reference substance solution and need testing solution 1～30 μ l respectively, inject chromatograph of liquid, measure; In the test sample chromatograph, have the chromatographic peak consistent with the reference substance chromatographic retention;

F. the chemical property discrimination method of flavones ingredient in the preparation:

It is an amount of that children's likes the food preparation, adds water and make dissolving, filters, and filtrate adds ethyl acetate, and jolting is extracted, and gets ethyl acetate liquid, and evaporate to dryness, residue add methanol makes dissolving, filters, and filtrate adds that magnesium powder is a small amount of to drip with the salt acid number, apparent cherry red.

Specifically: the discrimination method of described preparation comprises following all or part of content:

A. the thin layer chromatography discrimination method of the Rhizoma Atractylodis Macrocephalae in the preparation:

Getting children's, to like the food preparation an amount of, and the jolting that adds diethyl ether is extracted, and ether solution volatilizes, and residue adds methanol 1ml makes dissolving, as need testing solution; Other gets Rhizoma Atractylodis Macrocephalae control medicinal material, decocts with water, and filters, and filtrate is extracted with the ether jolting, and ether solution volatilizes, and residue adds methanol makes dissolving, in contrast medical material solution; Drawing each 10 μ l of above-mentioned two kinds of solution, put respectively on same silica gel g thin-layer plate, be developing solvent with chloroform-acetone-formic acid=9.5: 0.5: 0.06, launches, and taking-up is dried, and puts under the 365nm ultra-violet lamp and inspects; In the test sample chromatograph, with the corresponding position of control medicinal material chromatograph on, show the fluorescence speckle of same color;

B. one or both thin layer chromatography discrimination method in Fructus Crataegi, the ursolic acid in the preparation:

Get children's like the food preparation an amount of, the 30ml that adds diethyl ether, supersound process 10 minutes is put cold, filter petroleum ether=30～60 ℃ immersion 2 times of filtrate evaporate to dryness, residue, each 5ml, soak half a minute approximately, the petroleum ether that inclines, residue add methanol 1ml makes dissolving, as need testing solution; Other gets Fructus Crataegi control medicinal material 1g, and it is an amount of to add water, decocts about 30 minutes, filters, and the filtrate 30ml that adds diethyl ether extracts, and divides and gets ether solution, volatilizes, and residue adds methanol 1ml makes dissolving, in contrast medical material solution; Get the ursolic acid reference substance again, add methanol and make the solution that every 1ml contains 1mg, in contrast product solution; Draw one or both each the 5 μ l in above-mentioned need testing solution and the contrast solution, put respectively in same be on the silica gel g thin-layer plate of adhesive with the sodium carboxymethyl cellulose, with chloroform-acetone=13: 1 was developing solvent, launch, take out, dry, spray is with 10% ethanol solution of sulfuric acid, and it is clear to be heated to the speckle colour developing at 105 ℃; In the test sample chromatograph, with contrast chromatograph corresponding position on, show the speckle of same color;

C. the thin layer chromatography discrimination method of Fructus Hordei Germinatus in the preparation:

Get children's like the food preparation an amount of, add dehydrated alcohol 30ml, supersound process 40 minutes, filter, filtrate adds 50% potassium hydroxide solution 1ml, reflux 20 minutes, cooling rapidly, in the dislocation separatory funnel, water 20ml washing container, washing liquid is incorporated in the separatory funnel, with petroleum ether=30～60 ℃ extraction 2 times, each 20ml, divide and get petroleum ether layer, volatilize, residue adds ethyl acetate 1ml makes dissolving, as need testing solution; Other gets Fructus Hordei Germinatus control medicinal material 5g, shines medical material solution in pairs with legal system; Drawing each 10 μ l of above-mentioned two kinds of solution, put respectively on same silica gel g thin-layer plate, is developing solvent with benzene-chloroform=1: 1, launch, take out, dry, spray is with 50% alcoholic solution of 15% nitric acid, and it is clear to be heated to the speckle colour developing at 100 ℃, puts under the 365nm ultra-violet lamp and inspects; In the test sample chromatograph, with the corresponding position of control medicinal material chromatograph on, show the fluorescence speckle of same color;

D. the thin layer chromatography discrimination method of Fructus Aurantii in the preparation:

Getting children's, to like the food preparation an amount of, adds ethanol 10ml, and reflux 20 minutes filters, and filtrate is waved to 1ml, as need testing solution; Other gets Fructus Aurantii control medicinal material 2g, shines medical material solution in pairs with legal system; Drawing each 10 μ l of above-mentioned two kinds of solution, put respectively on same silica gel g thin-layer plate, be developing solvent with cyclohexane extraction-chloroform-ethyl acetate=20: 5: 5, launches, and taking-up is dried, and puts under the 365mm ultra-violet lamp and inspects; In the test sample chromatograph, with the corresponding position of control medicinal material chromatograph on, show the fluorescence speckle of same color;

E. the high performance liquid chromatography discrimination method of naringin in the preparation:

It is an amount of that children's likes the food preparation, puts in the apparatus,Soxhlet's, and it is an amount of to add methanol, reflux 4 hours, and the extracting solution evaporate to dryness, residue adds dissolve with methanol, filters, and gets subsequent filtrate as need testing solution; It is an amount of to get the naringin reference substance, adds methanol and makes reference substance solution; With octadecylsilane chemically bonded silica is filler; Methanol-water-glacial acetic acid=35: 65: 0.4 is a mobile phase; The detection wavelength is 283nm, and number of theoretical plate calculates by the naringin peak should be not less than 1000; Draw each 10 μ l of reference substance solution and need testing solution respectively, inject chromatograph of liquid, measure; In the test sample chromatograph, have the chromatographic peak consistent with the reference substance chromatographic retention;

F. the chemical property discrimination method of flavones ingredient in the preparation:

It is an amount of that children's likes the food preparation, adds water 50ml and make dissolving, filters, and filtrate adds ethyl acetate 20ml, and jolting is extracted, and gets ethyl acetate liquid, and evaporate to dryness, residue add methanol 2ml makes dissolving, filters, and filtrate adds that magnesium powder is a small amount of to drip with the salt acid number, apparent cherry red.

The content assaying method of described preparation comprises following all or part of content:

A. the high-efficient liquid phase chromatogram process measuring method of naringin content in the preparation:

Getting children's, to like the food preparation an amount of, accurate claims surely, adds methanol or ethanol extraction, filters, and gets subsequent filtrate and regulate concentration, as need testing solution; The naringin reference substance that is dried to constant weight of learning from else's experience is an amount of, accurately claims surely, adds methanol or ethanol is made reference substance solution; With octadecylsilane chemically bonded silica or eight alkyl silane bonded silica gels or dialkyl silane bonded silica gel is filler; Methanol or acetonitrile-water-acetic acid or formic acid or phosphoric acid=5～35: 15～65: 0～5 is mobile phase; The detection wavelength is one or several among 190～140nm, and number of theoretical plate calculates by the naringin peak should be not less than 1000; Draw reference substance solution and need testing solution 1～30 μ l respectively, inject chromatograph of liquid, measure, promptly; Children's to be measured likes food formulation content limit and should be: one to five years old child once likes the food preparation with children's and contains Fructus Aurantii with naringin C ₂₇H ₃₂O ₁₄Meter must not be less than 1.0mg;

B. the assay method of ursolic acid content in the preparation:

It is an amount of that children's likes the food preparation, the accurate title, decide, add diethyl ether or chloroform or ethyl acetate or acetone or alcohol or methanol extraction, the extracting solution evaporate to dryness, residue soaks with petroleum ether, petroleum ether inclines, residue adds dehydrated alcohol or methanol-chloroform=0～3: 0～2 mixed liquor is an amount of, and slight fever makes dissolving, standardize solution, shake up, as need testing solution; It is an amount of that in addition precision takes by weighing the ursolic acid reference substance, adds dehydrated alcohol or methanol is made reference substance solution; Accurate need testing solution 1～30 μ l that draws, respectively the cross point is on same silica gel g thin-layer plate, and with cyclohexane extraction or normal hexane-chloroform or dichloromethane-ethyl acetate or butyl acetate or Ethyl formate-formic acid or acetic acid=5～20: be developing solvent at 1～5: 1～8: 0～1, expansion, take out, dry, spray is with ethanol solution of sulfuric acid, and it is clear to be heated to speckle colour developing, scan according to thin layer chromatography scanning, measure test sample trap integrated value and reference substance trap integrated value, calculate, promptly; Children's to be measured likes food formulation content limit and should be: one to five years old child once likes the food preparation with children's and contains Fructus Crataegi with ursolic acid C ₃₀H ₄₈O ₃Meter must not be less than 0.5mg.

The content assaying method of described preparation comprises following all or part of content:

A. the high-efficient liquid phase chromatogram process measuring method of naringin content in the preparation:

Getting children's, to like the food preparation an amount of, accurate claims surely, puts in the apparatus,Soxhlet's, it is an amount of to add methanol, and reflux 4 hours is in the extracting solution dislocation evaporating dish, add an amount of washing container of methanol, merge extractive liquid, and washing liquid, evaporate to dryness, residue adds methanol and dissolves in right amount and be transferred in the 25ml measuring bottle, add methanol and be diluted to scale, shake up, filter, filter with 0.45 μ m microporous filter membrane, get subsequent filtrate as need testing solution; The 60 ℃ of drying under reduced pressure of learning from else's experience are an amount of to the naringin reference substance of constant weight, and accurate the title decides, and adds methanol and makes the solution that every 1ml contains 50 μ g, promptly; With octadecylsilane chemically bonded silica is filler; Methanol-water-glacial acetic acid=35: 65: 0.4 is a mobile phase; The detection wavelength is 283nm, and number of theoretical plate calculates by the naringin peak should be not less than 1000; Accurate respectively reference substance solution and each 10 μ l of need testing solution of drawing inject chromatograph of liquid, measure, promptly; Children's to be measured likes food formulation content limit and should be: one to five years old child once likes the food preparation with children's and contains Fructus Aurantii with naringin C ₂₇H ₃₂O ₁₄Meter must not be less than 2.0mg;

B. the assay method of ursolic acid content in the preparation:

It is an amount of that children's likes the food preparation, and accurate the title decides, and puts in the apparatus,Soxhlet's, it is an amount of to add diethyl ether, reflux 6 hours, and extracting solution reclaims ether to doing, residue soaks 2 times with 30～60 ℃ of petroleum ether, and each 5ml soaked 2 minutes, the petroleum ether that inclines, residue add that dehydrated alcohol-chloroform=mixed liquor was an amount of in 3: 2, and slight fever makes dissolving, be transferred in the 5ml measuring bottle, and be diluted to scale, shake up, as need testing solution; It is an amount of that precision takes by weighing the ursolic acid reference substance in addition, adds dehydrated alcohol and make the solution that every 1ml contains 0.5mg, in contrast product solution; Accurate need testing solution 5 μ l, reference substance solution 4 μ l and the 8 μ l of drawing, the cross point is on same silica gel g thin-layer plate respectively, with cyclohexane extraction-chloroform-ethyl acetate-formic acid=20: 5: 8: 0.1 was developing solvent, launch, take out, dry, spray is with 10% ethanol solution of sulfuric acid, and it is clear to be heated to speckle colour developing at 110 ℃, take out, on lamellae, cover onesize glass plate, use immobilization with adhesive tape on every side, scan according to thin layer chromatography scanning, wavelength: λ s=530nm, measure test sample trap integrated value and reference substance trap integrated value, calculate, promptly; Children's to be measured likes food formulation content limit and should be: one to five years old child once likes the food preparation with children's and contains Fructus Crataegi with ursolic acid C ₃₀H ₄₈O ₃Meter must not be less than 1.0mg.

Compared with prior art, preparation provided by the invention; Comprise have that drug effect is rapid, bioavailability is high, effervescent tablet of characteristics such as easy to carry, good mouthfeel, oral liquid, gel etc., be specially adapted to the patient that the child can not swallow solid preparation; Drug absorption is complete, bioavailability height, good looking appearance, good fluidity, stable, little, the good mouthfeel of local irritation of release simultaneously; Product has apparent in view therapeutic effect for the common disease of children's such as anorexia, calcium deficiency; Overcome the problem that prior art, product exist; The method of preparation method provided by the invention and quality control, production that can accurate instruction enterprise, guarantee product effectively; Reached the purpose of invention.

The applicant finds under study for action since we's the former powder of extractum not only mouthfeel is relatively poor, and hygroscopicity is very strong, conventional method is made effervescent tablet the moisture absorption can occur in put procedure, phenomenon such as disintegrate has voluntarily influenced the stability and the quality of product.And preparation is during gel, and its mouldability is very crucial.So the applicant has carried out lot of experiments, adjuvant, the technology of this product effervescent tablets, gel are screened.The applicant has carried out a series of experiments, with the supplementary product kind of the preparation technology that selects pharmaceutical preparation provided by the invention, use and consumption, ratio etc.; Guarantee its science, reasonable, feasible; The preparation that obtains has good disintegrating property and therapeutic effect.

Experimental example 1: Study on extraction

(1) factor is selected: the Chinese medicine extraction effect is subjected to the influence of factors such as solvent species, solvent load, extraction time, extraction time.Because of prior art extraction solvent species, extraction time, extraction time are investigated, so when medicinal material extract is investigated, choose solvent load as factor, the varying level of high spot reviews factor is to the influence of extraction effect.Take all factors into consideration the selection factor level in conjunction with aspects such as production cost, the energy.

(2) index is determined: select extractum recovery rate and naringin content as evaluation index, its reason and assay method are as follows:

1. extractum recovery rate: extractum is the material base of solid preparation performance curative effect, and its yield height directly influence preparation process, is reasonable, effective control device so be chosen as the extraction index.

2. assay: extractum recovery rate height can not reflect fully that active ingredient extracts situation, so measure the contained naringin content of extractum simultaneously as the Rhizoma Atractylodis Macrocephalae, Fructus Aurantii, Fructus Crataegi water boiling and extraction effect screening index.

(3) test:

1. dipping extracts the solvent load screening: take by weighing Massa Medicata Fermentata 77g, Fructus Hordei Germinatus 192g, Fructus Oryzae Germinatus 192g, totally 3 parts, add water boil after, 80～90 ℃ of dippings 2 hours, left standstill 4 hours, and filtered, filtrate decompression concentrates, vacuum drying, claim fixed dried cream weight, calculate the extractum recovery rate, result of the test sees the following form.

Dipping extracts solvent load and investigates table as a result

Tested number	Solvent load (doubly)	Dried cream heavy (g)	Extractum recovery rate (%)
				1	10	?143.28	31.08
2	12	?148.44	32.20
				3	14	?148.58	32.23

As seen from the above table, three kinds are extracted solvent load gained sample on the extractum recovery rate, 14 times of amount extraction effects are best, adopt 12 times of amounts and adopt 14 times of amount gained extractum amount difference little, illustrate that adding 12 times of water gagings can fully extract medical material, determine that therefore the optimised process of amount of water is 12 times of amounts.

2. decoct and extract the solvent load investigation: take by weighing Rhizoma Atractylodis Macrocephalae 195g, Fructus Aurantii 195g, Fructus Crataegi 295g, totally three parts, add not commensurability water boiling and extraction respectively, extract twice, each 1.5 hours, collecting decoction leaves standstill, and filters, filtrate decompression concentrates, vacuum drying, claim fixed dried cream weight, measure naringin content in the dried cream simultaneously, result of the test sees the following form.

Solvent load is investigated table as a result

Tested number	Solvent load (doubly)	Extractum recovery rate (%)	Naringin content (mg/g)
				1	6	32.16	3.42
2	8	35.41	3.85
				3	10	35.46	3.82

As seen from the above table, adopt 8 times of amounts and adopt 10 times of amount gained extractum amounts and content difference little, illustrate that adding 8 times of water gagings can fully extract medical material, determine that therefore the optimised process of amount of water is 8 times of amounts.

(4) demonstration test: by the front series of selection, optimize Massa Medicata Fermentata, Fructus Hordei Germinatus, the lixiviate of Fructus Oryzae Germinatus water temperature is got and the process conditions of the Rhizoma Atractylodis Macrocephalae, Fructus Aurantii, Fructus Crataegi water boiling and extraction, we carry out demonstration test, result such as following table after to the condition combination after preferred.

The condition demonstration test is got in the water temperature lixiviate

Medical material amount (g)	Cream heavy (g)	Extractum recovery rate (%)
			?461	?149.04	32.33
?461	?148.44	32.20
			?461	?148.95	32.31

From last table as seen, condition more stable (average paste-forming rate is 32.28%) is got in the water temperature lixiviate, illustrates that the experimental condition through screening is feasible.

Water boiling and extraction condition demonstration test

Medical material amount (g)	Cream heavy (g)	Yield of extract (%)	Naringin content (mg/g)
				?685	?243.52	35.55	3.82
?685	?243.04	35.48	3.78
				?685	?243.24	35.51	3.75

From last table as seen, the water boiling and extraction condition is more stable, and average paste-forming rate is 35.51%, and the naringin average content is 3.78mg/g, illustrates that the experimental condition through screening is feasible.

In sum, after Massa Medicata Fermentata, Fructus Hordei Germinatus, Fructus Oryzae Germinatus add 12 times of water gagings of water and boil in the side, extract at 80～90 ℃ of dippings; The Rhizoma Atractylodis Macrocephalae, Fructus Aurantii, Fructus Crataegi add 8 times of water gagings and decoct extraction, each 1.5 hours.

Experimental example 2: Study on Forming

2.1 effervescent tablet

(1) hygroscopicity is investigated: we carry out hygroscopicity investigation test to extracting ointment, the results are shown in following table.

The wettability test result

Sample		Extract powder	Extract powder
				The weighing bottle numbering		1	2
Weight of material (g)		4.99	5.02
				Moisture absorption blanking time percentage (%)	1h	2.56	2.78
2h	5.17	5.73
			3h		6.84	6.51
4h	9.17	9.00
			6h		10.77	10.81
8h	12.64	12.10
			10h		14.11	13.92
12h	17.27	17.68
			24h		19.51	20.05
36h	23.02	23.16
			48h		27.24	27.96
72h	30.50	30.63
			84h		32.66	32.42

Investigate by wettability test, think that ointment has certain moisture.

(2) disintegrating agent is selected: make effervescent tablet and need add a certain amount of gas-producing disintegrant, so we investigate disintegrating agent, disintegrating agent commonly used at present has sodium bicarbonate, citric acid, fumaric acid etc., and its consumption is investigated, and result of the test sees the following form.

The disintegrating agent investigation table

Batch	Total weight of material (g)	Disintegrating agent			Disintegration time (branch)
						Sodium bicarbonate (g)	Citric acid (g)	Fumaric acid (g)
		1	100	50					24	-	4
2	100				50	12	-	7
		3	100	50					-	6	8
4	100				50	12	6	3

From above-mentioned test as can be seen, it is the shortest to add the effervescent tablet disintegration time that 50% sodium bicarbonate, 12% citric acid and 6% tartaric acid makes, thus we to select the disintegrating agent addition be sodium bicarbonate 50%, citric acid 12%, tartaric acid 6%.

(3) the mobile investigation: for guaranteeing that divided dose is accurate, require the good flowability of fill material tool, so to measure the flowability of method examination angle of repose mixed material.

Fixed funnel method: get 3 funnel series connection, lowermost end is apart from horizontal positioned graph paper 1.5cm place, carefully fill material is poured into along hopper walls in the funnel of going up most till the granule cone tip that bottom funnel forms touches the funnel end opening, measure the diameter of conical base by graph paper, (tg α=H/R/2), result of calculation sees the following form to calculate angle of repose.

α angle of repose of fill material

Working sample	Angle of repose
		1	55.3°
2	54.4°
		3	55.8°

As seen from the above table, mixed material angle of repose＞40 ° show that promptly the mixed material property that flows is bad, can not satisfy the branch reload request.

(3) diluent is selected: consider the character of extract, we select for use lactose as diluent, while lactose tool no hygroscopicity, and compressibility is good, characteristics such as stable in properties.

(4) fluidizer is investigated: from top angle of repose measurement result as can be seen, mixed material mobile relatively poor, divided dose is accurate when guaranteeing tabletting, need to increase the flowability of material, we select fluidizer magnesium stearate commonly used as fluidizer for this reason, and its consumption investigated, result of the test sees the following form.

Magnesium stearate consumption investigation table

Sequence number	Consumption (%)	Angle of repose (°)
			1	0.2	46.5
2	0.5	33.1
			3	1	32.3

From top result as can be seen, consumption was at 0.2% o'clock, improve to some extent the angle of repose to mixed material, but can't meet the demands, consumption is 0.5% and can reaches requirement at 1% o'clock, but the magnesium stearate consumption is 1% o'clock, and angle of repose, decline scope was not clearly, so we determine that the magnesium stearate consumption is 0.5%.

(5) correctives is selected: because our mouthfeel is bitter, so need to add an amount of sedan-chair flavor agent, with reference to relevant document with according to the character of sedan-chair flavor agent, chooses steviosin and do the agent of sedan-chair flavor, is 0.2% by trial test selection correctives addition.

(6) film-making pressure determines

Effervescent tablet should disintegrate and stripping in the short as far as possible time, so tablet hardness is littler than ordinary tablet, the disintegrate fast to guarantee slice, thin piece that enough porositys are arranged, but can keep outward appearance again, improve fineness.Adopt optimizing prescriptions to be prepared into the effervescent tablet of different hardness, the results are shown in following table.

Hardness is to the influence of disintegration time

Pressure (Kg/cm 2)	3.0	4.0	5.0	6.0	7.0
						Average disintegration (s)	Can't molding	170	182	250	320

As seen from the above table, pressure is at 4.0～5.0Kg/cm ²Better.

(7) critical relative humidity (CRH) is measured

Material can be loaded smoothly in order to guarantee to produce, and the humidity that need control environment so that make material have good mobility, makes loading amount even, and we carry out equilibrium hygroscopicity test, i.e. moisture equilibrium at dry side curve determination to mixed material for this reason.Get totally six parts of the about 1g of mixed material, put in the weighing botle, the accurate title, decide, the weighing bottle cap is opened, put into relative humidity respectively and be 20%, 33%, 43%, 60%, 75%, 92% environment, in 25 ℃ of constant incubators, placed 84 hours, take out weighing botle, it is fixed to add a cover the accurate title in back, calculating the moisture absorption percentage rate, is abscissa with the relative humidity, and the moisture absorption percentage rate is a vertical coordinate, draw the moisture equilibrium at dry side curve and see accompanying drawing 1, result of the test sees the following form.

The moisture absorption percentage rate (%) of mixed material

The saturated salt solution kind	Relative humidity (%)	Moisture absorption percentage rate (%)
			CH 3COOK·1.5H 2O	20	2.78
MgCl 2·6H 2O	33	3.65
			K 2CO 3·2H 2O	43	5.61
NaBr·2H 2O	60	13.21
			NaCl	75	23.64
KNO 3	92	45.02

To moisture absorption percentage rate (%) mapping, the results are shown in accompanying drawing 1 with relative humidity (CRH).Know that by figure its CRH% is about 60%, prompting is when tabletting, packing, storage, and envionmental humidity should be controlled at below 60%, to guarantee stability of formulation.

2.2 gel

(1) selection of matrix species

Substrates quantity % mouldability gelling performance

Kappa-carrageenan 0.8 good crisp, difference of hardness

Lambda-carrageenan 0.8 difference is gel not

Konjac glucomannan 0.8 difference is gel not

Kappa-carrageenan+Konjac glucomannan 0.8 good hardness is moderate, and tough elasticity is better

Lambda-carrageenan+Konjac glucomannan 0.8 difference is gel not

(2) screening of substrate composition and consumption

Konjac glucomannan: kappa-carrageenan consumption % gelling performance

1: 2 1.0 crisp, difference of hardness, poor toughness, no bite

1: 2 0.8 crisp, difference of hardness, poor toughness, no bite

1: 3 1.0 crisp, difference of hardness, poor toughness, no bite

Hardness was moderate in 1: 3 0.8, and tough good springiness has bite

Hardness was moderate in 2: 3 1.0, and tough elasticity is better, and bite is arranged

Hardness was big in 2: 3 0.8, and tough elasticity is strong

The result shows: the best composite condition of substrate is a Konjac glucomannan: the part by weight of kappa-carrageenan=1: 3, consumption is 0.8%.

Experimental example 3: pharmacodynamic experiment

3.1 rat gastric acid, pepsic influence

Get 50 of rats, body weight 180 ± 20g, male and female half and half, evenly be divided into 5 groups at random: the normal saline group, the XIAOER XISHI PIAN group, the XIAOER XISHI TANGJIANG group, effervescent tablet group of the present invention, micropill group of the present invention, gastric infusion reaches the normal saline with volume respectively, administration every day 1 time, successive administration 7 days, in rat fasting (can't help water) in the 6th day, in the end 2h dissects rat abdomen with 20% urethane anesthetized rat (rat fasting 12h) after 1 administration, and inserting people's one diameter from the pylorus end to gastric is the plastic tube of 3mm, near the pylorus ball place's ligation fix, to collect gastric juice.And insert people's glandular stomach through esophagus with a plastic tube by the oral cavity, and with the esophagus ligation,, carry out the gastric perfusion with peristaltic pump by the speed of 12ml/h and collect with 35 ℃ of left and right sides normal saline, it is standby to collect the 1h eluate.

1. to the influence of rat stomach proteinase activity

The hydrochloric acid 2ml that gets eluate 2ml and 0.1mol is in test tube, mixing, adding three of people's protein pipe in every test tube (gets interior through 1mm, long 10cm, the capillary tube of even thickness makes it fill fresh Ovum Gallus domesticus album with siphonage, no bubble in the pipe, be placed on then in 80 ℃ of left and right sides water heaters and make protein coagulation, take out standby).With the good test tube mouth of defat tampon, in 37 ± 1 ℃ of calorstats, hatch 24h, measure protein pipe and soak the length (mm) that the people holds transparent part, get three end sums and ask its meansigma methods, calculate peptic activity of stomach (pepsin unit=meansigma methods ²* 16), the results are shown in following table.

Influence to the rat stomach prolease activity

Group	Number of animals (only)	Dosage (g/kg)	Peptic activity of stomach (u)
				Normal saline group XIAOER XISHI PIAN group XIAOER XISHI TANGJIANG group effervescent tablet group of the present invention micropill group of the present invention	10 10 10 10 10	- 4 4 4 4	27.8±11.5 48.2±16.2 50.6±12.8 53.5±19.5 58.7±8.4

As can be seen from the above table, the effect of effervescent tablet of the present invention, micropill of the present invention raising pepsin vigor is better than commercially available XIAOER XISHI PIAN and XIAOER XISHI TANGJIANG.

2. to the influence of rat free acid and total acidity

Get eluate 2ml, put in the small beaker, add each 3 of people's dimethyl yellow indicator and phenolphthalein indicators, mixing,, cherry red be, serve as a contrast a blank sheet of paper down in small beaker and drip people NaOH volumetric solution (0.02mol/L) titration slowly with microburet, to the redness that phenolphthalein occurs (color is no longer deepened), remember its amount, be total acidity terminal point amount with last used NaOH sum, be calculated as follows free acid and total acidity, the results are shown in following table.

Gastric juice amount * 1000 are got in free acid mol/L=(0.02 * free acid terminal point amount)/titration

Gastric juice amount * 1000 are got in total acidity mol/L=(0.02 * total acidity terminal point amount)/titration

Influence to stomach free acid and total acid content

Group	Number of animals (only)	Dosage (g/kg)	Free acid amount (mol/L)	Total acid content (mol/L)
					Normal saline group XIAOER XISHI PIAN group XIAOER XISHI TANGJIANG group effervescent tablet group of the present invention micropill group of the present invention	10 10 10 10 10	- 4 4 4 4	5.3±3.6 9.5±2.4 9.9±3.8 10.8±4.5 11.1±1.4	11.2±4.5 16.2±3.4 16.9±4.1 18.5±5.2 19.7±2.7

As can be seen from the above table, the effect of effervescent tablet of the present invention, micropill of the present invention raising stomach free acid amount and total acid content is better than commercially available XIAOER XISHI PIAN and XIAOER XISHI TANGJIANG.

3.2 research to the intestinal propulsion effect

Get 50 of Kunming mouses, body weight 20? g, male and female half and half, fasting (can't help water) 12h before the experiment.Mice evenly is divided into 5 groups at random: matched group, XIAOER XISHI PIAN group, XIAOER XISHI KELI group, effervescent tablet group of the present invention, micropill group of the present invention.Matched group gives 0.5% sodium carboxymethyl cellulose, all irritates stomach by body constitution amount 20ml/kg.30min gives the suspension that each Mus 5% carbon is not made with 10% arabic gum behind the medicine, irritates stomach by body constitution amount 10ml/kg, puts to death animal with the cervical vertebra dislocation method behind the 20min, carve abdomen immediately, with the digestive tract complete excision of pylorus to rectum end, do not add traction, be tiled on the glass plate.Measure intestinal total length and carbon powder forward position distance, calculate the percentage rate that the latter accounts for the intestinal total length to pylorus.Computing formula is: advance percentage rate (%)=charcoal forward position, end and pylorus distance (cm)/small intestinal total length (pylorus is to ileocecus) (cm) * 100%.Concrete outcome sees the following form.

Influence to the mouse small intestine progradation

Group	Number of animals (only)	Dosage (g/kg)	The charcoal end advances (%)
				Control group children Xishi Tablet group	10 10	- 8	59.4±11.8 65.1±9.6

XIAOER XISHI KELI group effervescent tablet group of the present invention micropill group of the present invention

10 10 10

8 8 8

67.8±5.7 70.2±10.6 71.5±8.2

The result shows, the effect that effervescent tablet of the present invention, micropill of the present invention make mouse intestinal carbon powder fltting speed significantly increase (to account for intestinal percentage ratio be not index in the forward position with carbon) is better than commercially available XIAOER XISHI PIAN and XIAOER XISHI KELI.

Experimental example 4: the research of method of quality control

4.1 the thin layer chromatography discrimination method of the Rhizoma Atractylodis Macrocephalae in the capsule

The purpose of this experiment is the feature for the outstanding Rhizoma Atractylodis Macrocephalae, and for getting rid of in the preparation and the similarly interference of other composition of Rhizoma Atractylodis Macrocephalae ingredient, the experimenter compares test respectively to sample extraction, development system, coloration method etc.; Through screening, determined the best approach and condition: getting children's, to like the food effervescent formulation an amount of, and the jolting that adds diethyl ether is extracted, and ether solution volatilizes, and residue adds methanol 1ml makes dissolving, as need testing solution; Other gets Rhizoma Atractylodis Macrocephalae control medicinal material, decocts with water, and filters, and filtrate is extracted with the ether jolting, and ether solution volatilizes, and residue adds methanol makes dissolving, in contrast medical material solution; Test according to thin layer chromatography (an appendix VI of Chinese Pharmacopoeia version in 2005 B), draw each 10 μ l of above-mentioned two kinds of solution, put respectively on same silica gel g thin-layer plate, with chloroform-acetone-formic acid (9.5: 0.5: 0.06) is developing solvent, launch, take out, dry, put under the ultra-violet lamp (365nm) and inspect; In the test sample chromatograph, with the corresponding position of control medicinal material chromatograph on, show the fluorescence speckle of same color.

The thin layer chromatography discrimination method of the Rhizoma Atractylodis Macrocephalae in the capsule

Developing solvent	Lamellae	Effect
			Benzol-cyclohexane-ethanol-formic acid (12: 6: 1: 1)	Silica gel G	Difference: sample separation is unintelligible
Toluene-butyl acetate-methanol (8: 5: 3)	Silica gel G	Difference: feminine gender has interference, the speckle hangover
			Dichloromethane-butanone-formic acid (5: 1: 0.5)	Silica gel H	Preferable: it is clear that sample and reference substance all separate, negative noiseless
Chloroform-acetone-formic acid (9.5: 0.5: 0.06)	Silica gel G	Best: it is clear, negative noiseless that sample and reference substance all separate

4.2 content of baicalin is measured in the effervescent tablet

Test is reference substance with the naringin, with Alltech P426 high performance liquid chromatograph, has established children's and has liked the best approach of eating the assay of naringin in the effervescent tablet:

The chromatographic condition octadecylsilane chemically bonded silica is a filler; Methanol-water-glacial acetic acid (35: 65: 0.4) is a mobile phase; The detection wavelength is 283nm, and number of theoretical plate calculates by the naringin peak should be not less than 1000.

The preparation of the reference substance solution 60 ℃ of drying under reduced pressure of learning from else's experience are an amount of to the naringin reference substance of constant weight, accurately claim surely, add methanol and make the solution that every 1ml contains 50 μ g, promptly.

This product under the weight differential item is got in the preparation of need testing solution, and porphyrize is got about 2.0g, the accurate title, decide, and puts in the apparatus,Soxhlet's, and it is an amount of to add methanol, reflux 4 hours in the extracting solution dislocation evaporating dish, adds an amount of washing container of methanol, merge washing liquid, evaporate to dryness, residue add methanol and dissolve in right amount and be transferred in the 25ml measuring bottle, add methanol and be diluted to scale, shake up, filter, filter with microporous filter membrane (0.45 μ m), get subsequent filtrate as need testing solution.

Accurate respectively reference substance solution and each the 10 μ l of need testing solution of drawing of algoscopy inject chromatograph of liquid, measure, promptly.

Every of this product contains Fructus Aurantii with naringin (C ₂₇H ₃₂O ₁₄) meter, must not be less than 2.0mg.

This method has been passed through following methodological study test respectively:

The test of 1 negative ELIMINATION OF ITS INTERFERENCE is for investigating the mensuration whether other medical material and adjuvant disturb naringin, except that Fructus Aurantii, takes by weighing other medical material and adjuvant in the prescription ratio and makes negative control solution with method and measure.The result shows, and is negative noiseless to the assay of naringin.

It is an amount of that the investigation precision of 2 linear relationships takes by weighing the naringin reference substance, add mobile phase and make the solution that every 1ml contains 0.467mg, therefrom precision is measured 0.2ml, 0.4ml, 0.6ml, 0.8ml, 1.0ml, split in the 5ml measuring bottle, add methanol and be diluted to scale, shake up, be mixed with the reference substance solution of 0.01868mg/ml, 0.03736mg/ml, 0.05604mg/ml, 0.07472mg/ml, 0.09340mg/ml, the therefrom accurate respectively 10 μ l that draw, inject chromatograph of liquid, measure according to high performance liquid chromatography (an appendix VI of Chinese Pharmacopoeia version in 2000 D).With the peak area is abscissa, and the amount of naringin (μ g) is figure for vertical coordinate, the drawing standard curve.See the following form.

Regression equation: Y=0.0005X+0.0016

Correlation coefficient: γ=0.9999

The result shows that naringin sample size linear relationship between 0.1868 μ g～0.934 μ g is good.

Through calculating, the naringin standard curve is one to cross the straight line of initial point, therefore selects one point external standard method to measure the content that children's likes naringin in the food effervescent tablet.

The naringin linear relationship

Numbering	Peak area	Naringin amount (μ g)
			1	369.42	?0.1868
2	720.93	?0.3736
			3	1086.58	?0.5604
4	1458.87	?0.7472
			5	1810.40	?0.9340

Accurate reference substance solution (0.0467mg/ml) the 10 μ l that draw of 3 precision test inject chromatograph of liquid, the record peak area, and replication 5 times is investigated reference substance solution precision, and measurement result sees the following form.

The precision test

Test number (TN)	1	2	3	4	5	Meansigma methods	?RSD(％)
								Peak area	910.20	903.44	918.22	900.81	908.67	908.27	?0.74

The result shows that reference substance solution precision is good.

4 stability tests

4.1 accurate reference substance solution (0.0467mg/ml) the 10 μ l that draw of reference substance stability test inject chromatograph of liquid, the record peak area is measured at 0,2,4,6,8 hour sample introduction respectively, and measurement result sees the following form.

Table 4 reference substance stability test result

Time (h)	0	2	4	6	8	Meansigma methods	?RSD(％)
								Peak area	910.20	903.44	918.22	900.81	908.67	908.27	?0.74

The result shows that reference substance solution is good at 8 hours internal stabilities.

4.2 accurate need testing solution (80.534mg/ml) the 10 μ l that draw of need testing solution stability test inject chromatograph of liquid, measure at 0,2,4,6,8 hour sample introduction respectively, measurement result sees the following form.

Need testing solution stability test result

Time (h)	0	2	4	6	8	Meansigma methods	?RSD(％)
								Peak area	890.26	881.05	900.24	895.97	883.67	890.24	?0.91

The result shows that need testing solution is good at 8 hours internal stabilities.

5 replica tests are got this product under the weight differential item, and porphyrize is got about 2.0g (totally 5 parts), and precision claims fixed, by operating under preparation of text need testing solution and the mensuration item.The results are shown in Table 6.

Table 6 replica test

Numbering	1	2	3	4	5	Meansigma methods	?RSD(％)
								Content (mg/ sheet)	2.759	2.693	2.705	2.754	2.756	2.733	?1.17

The result shows that repeatability is good.

This product under the weight differential item is got in the test of 6 average recoveries, and (content of naringin: 0.5453mg/g), porphyrize is got about 1.0g (totally 6 parts), the accurate title, decide, split in the apparatus,Soxhlet's, precision is measured naringin reference substance (0.558mg/ml) 1.0ml respectively, splits in the above-mentioned apparatus,Soxhlet's, it is an amount of to add methanol, reflux 4 hours in the extracting solution dislocation evaporating dish, adds an amount of washing container of methanol, merge washing liquid, evaporate to dryness, residue add methanol and dissolve in right amount and be transferred in the 25ml measuring bottle, add methanol and are diluted to scale, shake up, filter, the accurate subsequent filtrate 10 μ l that draw inject chromatograph of liquid, measure, promptly.Measurement result sees the following form.(average sheet is heavy: 5.01239g)

The test of naringin average recovery

Numbering	Test sample weighing (g)	Pure product amount (mg) in the test sample	Naringin addition (mg)	The amount of recording (mg)	The response rate (%)
						1	1.03652	0.5652	0.558	1.1228	99.93
2	1.00467	0.5478	0.558	1.1088	100.54
						3	0.98963	0.5396	0.558	1.0871	98.12
4	0.99587	0.5430	0.558	1.0927	98.51
						5	1.05792	0.5769	0.558	1.1322	99.52
6	1.04061	0.5674	0.558	1.1209	99.19

Average recovery rate=99.30%, RSD=0.90%.

7 sample sizes are measured the preparation and the operation down of algoscopy item of pressing the text need testing solution, and working sample the results are shown in following table.

Ten batch sample assay results

Batch	Naringin (mg/ sheet)
		1	2.813
2	3.226
		3	2.435
4	3.869
		5	2.361
6	3.458
		7	3.062
8	2.637
		9	2.554
10	2.832

Conclusion: every of this product contains Fructus Aurantii with naringin (C ₂₇H ₃₂O ₁₄) meter, must not be less than 2.0mg.

Description of drawings: accompanying drawing 1 is a critical relative humidity curve chart of the present invention.

Concrete embodiment:

Embodiments of the invention 1: Massa Medicata Fermentata 385g, Fructus Aurantii 195g, Rhizoma Atractylodis Macrocephalae 195g, Fructus Crataegi 295g, Fructus Oryzae Germinatus 960g, Fructus Hordei Germinatus 960g

Get Massa Medicata Fermentata, Fructus Hordei Germinatus, Fructus Oryzae Germinatus and add behind the water boil, left standstill 4 hours, filter filtrate for later use 80～90 ℃ of dippings 2 hours; Other gets the Rhizoma Atractylodis Macrocephalae, Fructus Aurantii, Fructus Crataegi and decocts with water secondary, and each 1.5 hours, collecting decoction, leave standstill, filter, filtrate and above-mentioned filtrate merge, relative density is 1.20 clear paste when being concentrated into 50 ℃, drying under reduced pressure is pulverized the cream powder that sieves, the cream powder adds sodium bicarbonate 2500g, citric acid 600g, fumaric acid 300g, steviosin 10g, magnesium stearate 25g, lactose 550g, mix homogeneously, tabletting, pressure is at 4.0～5.0Kg/cm ², promptly get effervescent tablet, this product oral, one to five years old one time a slice, one full year of life successively decreased with interior, three times on the one with two of last times in five years old.

Embodiments of the invention 2: Massa Medicata Fermentata 385g, Fructus Aurantii 195g, Rhizoma Atractylodis Macrocephalae 195g, Fructus Crataegi 295g, Fructus Oryzae Germinatus 960g, Fructus Hordei Germinatus 960g

Get Massa Medicata Fermentata, Fructus Hordei Germinatus, Fructus Oryzae Germinatus and add behind the water boil, left standstill 4 hours, filter filtrate for later use 80～90 ℃ of dippings 2 hours; Other gets the Rhizoma Atractylodis Macrocephalae, Fructus Aurantii, Fructus Crataegi decocts with water secondary, and each 1.5 hours, collecting decoction, leave standstill, filter, filtrate and above-mentioned filtrate merge, relative density is 1.20 clear paste when being concentrated into 50 ℃, and drying under reduced pressure is pulverized, the cream powder that sieves adds an amount of starch, with 80% ethanol and 1.2% soybean oil system soft material, the soft material that makes micropill mechanism ball, wet feed pushed the 0.8mm sieve aperture, the wet grain of strip cuts off round as a ball, 50～60 ℃ of drying and mouldings are crossed 16～20 mesh sieves and are selected ball or merge above-mentioned four kinds of clear paste, spray drying, wet-milling granulation molding, mould placed add the great achievement ball in the coating pan, medicated powder: water is 1: 1.2, and the coating pan rotating speed is 30r/min, capping, select ball, coating, coating material are 5% acrylic resin II number, during 40 ℃ of sheet bed tempertaures, jet velocity 6.0ml/kgmin promptly gets pellet.

Embodiments of the invention 3: Massa Medicata Fermentata 385g, Fructus Aurantii 195g, Rhizoma Atractylodis Macrocephalae 195g, Fructus Crataegi 295g, Fructus Oryzae Germinatus 960g, Fructus Hordei Germinatus 960g

Get Massa Medicata Fermentata, Fructus Hordei Germinatus, Fructus Oryzae Germinatus and add behind the water boil, left standstill 4 hours, filter filtrate for later use 80～90 ℃ of dippings 2 hours; Other gets the Rhizoma Atractylodis Macrocephalae, Fructus Aurantii, Fructus Crataegi and decocts with water secondary, and each 1.5 hours, collecting decoction left standstill, and filters, and filtrate merges with above-mentioned filtrate, and relative density is 1.20 clear paste when being concentrated into 50 ℃, and the adding distilled water promptly gets oral liquid.

Embodiments of the invention 4: Massa Medicata Fermentata 385g, Fructus Aurantii 195g, Rhizoma Atractylodis Macrocephalae 195g, Fructus Crataegi 295g, Fructus Oryzae Germinatus 960g, Fructus Hordei Germinatus 960g

Get Massa Medicata Fermentata, Fructus Hordei Germinatus, Fructus Oryzae Germinatus and add behind the water boil, left standstill 4 hours, filter filtrate for later use 80～90 ℃ of dippings 2 hours; Other gets the Rhizoma Atractylodis Macrocephalae, Fructus Aurantii, Fructus Crataegi and decocts with water secondary, and each 1.5 hours, collecting decoction left standstill, and filters filtrate and the merging of above-mentioned filtrate; Konjac glucomannan, K-carrageenan are pressed 1: 3 part by weight mix homogeneously, under stirring condition, 0.8% mixed-matrix is put into cold water, make it to disperse, soak 20～30min, make the abundant water absorption and swelling of substrate, stir then, heat, filter, filter the back and under condition of stirring, add herbal extract, mix homogeneously joins rapidly in the disinfecting container, seals, sterilization is cooled to about 30 ℃ condensation rapidly, drying promptly gets gel.

Embodiments of the invention 5: the thin layer chromatography discrimination method of the Rhizoma Atractylodis Macrocephalae in the granule

Getting children's, to like the food effervescent formulation an amount of, adds the ethyl acetate jolting and extract, and extracting solution volatilizes, and residue adds ethanol makes dissolving, as need testing solution; Other gets Rhizoma Atractylodis Macrocephalae control medicinal material, decocts with water, and filters, and filtrate adds the ethyl acetate jolting extracts, and extracting solution volatilizes, and residue adds ethanol makes dissolving, in contrast medical material solution; According to thin layer chromatography (appendix VIB of Chinese Pharmacopoeia version in 2005) test, draw each 30 μ l of above-mentioned two kinds of solution, put respectively on same silica gel g thin-layer plate, with dichloromethane-butanone-formic acid (1: 2: 1) is developing solvent, launches, and takes out, dry, put under the ultra-violet lamp (365nm) and inspect; In the test sample chromatograph, with the corresponding position of control medicinal material chromatograph on, show the fluorescence speckle of same color.

Embodiments of the invention 6: the thin layer chromatography discrimination method of the Rhizoma Atractylodis Macrocephalae in the drop pill

Getting children's, to like the food effervescent pills an amount of, and the jolting that adds diethyl ether is extracted, and ether solution volatilizes, and residue adds methanol 1ml makes dissolving, as need testing solution; Other gets Rhizoma Atractylodis Macrocephalae control medicinal material, decocts with water, and filters, and filtrate is extracted with the ether jolting, and ether solution volatilizes, and residue adds methanol makes dissolving, in contrast medical material solution; Test according to thin layer chromatography (an appendix VI of Chinese Pharmacopoeia version in 2005 B), draw each 10 μ l of above-mentioned two kinds of solution, put respectively on same silica gel g thin-layer plate, with chloroform-acetone-acetic acid (10: 2: 0.1) is developing solvent, launch, take out, dry, put under the ultra-violet lamp (254nm) and inspect; In the test sample chromatograph, with the corresponding position of control medicinal material chromatograph on, show the fluorescence speckle of same color.

Embodiments of the invention 7: the thin layer chromatography discrimination method of Fructus Crataegi in the capsule

Getting children's, to like food effervescent capsule 's content an amount of, adds the ethyl acetate supersound extraction, the extracting solution evaporate to dryness, and residue soaks with petroleum ether, the petroleum ether that inclines, residue ethanol makes dissolving, as need testing solution; Other gets the Fructus Crataegi control medicinal material, decocts with water, and the decocting liquid ethyl acetate extraction, extracting solution volatilizes, and residue ethanol makes dissolving, in contrast medical material solution; According to thin layer chromatography (an appendix VI of Chinese Pharmacopoeia version in 2005 B) test, draw each 1 μ l of above-mentioned solution, put respectively in same GF ₂₅₄On the lamellae, toluene-ethyl acetate-formic acid (20: 4: 0.5) is developing solvent, and spray is with 30% ethanol solution of sulfuric acid, and 80 ℃ to be heated to speckle colour developing clear; In the test sample chromatograph, with control medicinal material chromatograph or the corresponding position of reference substance chromatograph on, show the speckle of same color.

Embodiments of the invention 8: the thin layer chromatography discrimination method of Fructus Crataegi in the drop pill

Getting children's, to like the food effervescent pills an amount of, adds alcohol reflux, puts coldly, filter, and the filtrate evaporate to dryness, residue soaks 2 times with petroleum ether, each 5ml (soaking half a minute approximately), the petroleum ether that inclines, residue add methanol makes dissolving, as need testing solution; Get the ursolic acid reference substance again, add methanol and make reference substance solution; Test according to thin layer chromatography (appendix VIB of Chinese Pharmacopoeia version in 2005), draw each 30 μ l of above-mentioned solution, put respectively in same be on the silica gel g thin-layer plate of adhesive with the sodium carboxymethyl cellulose, with chloroform-acetone (1: 1) is developing solvent, launch, take out, dry, spray is with 10% ethanol solution of sulfuric acid, and it is clear to be heated to the speckle colour developing at 105 ℃; In the test sample chromatograph, with control medicinal material chromatograph and the corresponding position of reference substance chromatograph on, show the speckle of same color.

Embodiments of the invention 9: the thin layer chromatography discrimination method of Fructus Hordei Germinatus in the capsule

Getting children's, to like food effervescent capsule 's content an amount of, adds methanol extraction, and extracting solution adds 40% sodium hydroxide solution, reflux, cooling rapidly, in the dislocation separatory funnel, it is an amount of to add water, uses ether extraction, extracting solution volatilizes, and residue adds chloroform makes dissolving, as need testing solution; Other gets the Fructus Hordei Germinatus control medicinal material, shines medical material solution in pairs with legal system; Test according to thin layer chromatography (an appendix VI of Chinese Pharmacopoeia version in 2005 B), draw each 30 μ l of above-mentioned two kinds of solution, put respectively on same block of silica gel H lamellae, with toluene-chloroform (10: 1) is developing solvent, launches, and takes out, dry, spray is with 50% alcoholic solution of 20% nitric acid, and 105 ℃ to be heated to the speckle colour developing clear, puts under the ultra-violet lamp (254nm) and inspect; In the test sample chromatograph, with the corresponding position of control medicinal material chromatograph on, show the fluorescence speckle of same color.

Embodiments of the invention 10: the thin layer chromatography discrimination method of Fructus Hordei Germinatus in the granule

Get children's like the food effervescent granule an amount of, add dehydrated alcohol 30ml, supersound process 40 minutes, filter, filtrate adds 50% potassium hydroxide solution 1ml, reflux 20 minutes, cooling rapidly, in the dislocation separatory funnel, water 20ml washing container, washing liquid is incorporated in the separatory funnel, extracts 2 times with petroleum ether (30～60 ℃), each 20ml, divide and get petroleum ether layer, volatilize, residue adds ethyl acetate 1ml makes dissolving, as need testing solution; Other gets Fructus Hordei Germinatus control medicinal material 5g, shines medical material solution in pairs with legal system; Test according to thin layer chromatography (an appendix VI of Chinese Pharmacopoeia version in 2005 B), draw each 1 μ l of above-mentioned two kinds of solution, put respectively on same silica gel g thin-layer plate, with benzene-chloroform (1: 10) is developing solvent, launches, and takes out, dry, spray is with 50% alcoholic solution of 15% nitric acid, and it is clear to be heated to the speckle colour developing at 100 ℃, puts under the ultra-violet lamp (365nm) and inspects; In the test sample chromatograph, with the corresponding position of control medicinal material chromatograph on, show the fluorescence speckle of same color.

Embodiments of the invention 11: the thin layer chromatography discrimination method of Fructus Aurantii in the tablet

Getting children's, to like the food effervescent tablet an amount of, adds acetone 10ml, and reflux 20 minutes filters, and filtrate is waved to 1ml, as need testing solution; Other gets Fructus Aurantii control medicinal material 2g, shines medical material solution in pairs with legal system; Test according to thin layer chromatography (an appendix VI of Chinese Pharmacopoeia version in 2005 B), draw each 10 μ l of above-mentioned two kinds of solution, put respectively on same silica gel g thin-layer plate, with normal hexane-chloroform-ethyl acetate (20: 1: 1) is developing solvent, launch, take out, dry, put under the ultra-violet lamp (365mm) and inspect; In the test sample chromatograph, with the corresponding position of control medicinal material chromatograph on, show the fluorescence speckle of same color;

Embodiments of the invention 12: the thin layer chromatography discrimination method of Fructus Aurantii in the capsule

Getting children's, to like food effervescent capsule 's content an amount of, adds methanol 20ml, and supersound extraction filters, and filtrate is waved to 1ml, as need testing solution; Other gets Fructus Aurantii control medicinal material 2g, shines medical material solution in pairs with legal system; Test according to thin layer chromatography (an appendix VI of Chinese Pharmacopoeia version in 2005 B), draw each 30 μ l of above-mentioned two kinds of solution, put respectively on same silica gel g thin-layer plate, with normal hexane-chloroform-ethyl acetate (5: 3: 1) is developing solvent, launch, take out, dry, put under the ultra-violet lamp (254mm) and inspect; In the test sample chromatograph, with the corresponding position of control medicinal material chromatograph on, show the fluorescence speckle of same color.

Embodiments of the invention 13: the high performance liquid chromatography discrimination method of naringin in the granule:

It is an amount of that children's likes the food effervescent granule, adds methanol eddy, and extracting solution filters, and gets subsequent filtrate as need testing solution; It is an amount of to get the naringin reference substance, adds methanol and makes reference substance solution; With eight alkyl silane bonded silica gels is filler; Methanol-water-glacial acetic acid (5: 65: 5) is a mobile phase; The detection wavelength is 283nm, and number of theoretical plate calculates by the naringin peak should be not less than 1000; Draw each 20 μ l of reference substance solution and need testing solution respectively, inject chromatograph of liquid, measure; In the test sample chromatograph, have the chromatographic peak consistent with the reference substance chromatographic retention.

Embodiments of the invention 14: the high performance liquid chromatography discrimination method of naringin in the drop pill

It is an amount of that children's likes the food effervescent pills, adds ethanol ultrasonic extraction, and extracting solution filters, and gets subsequent filtrate as need testing solution; It is an amount of to get the naringin reference substance, adds ethanol and makes reference substance solution; With octadecylsilane chemically bonded silica is filler; Acetonitrile-water-phosphoric acid (35: 15: 0.05) is mobile phase; The detection wavelength is 365nm, and number of theoretical plate calculates by the naringin peak should be not less than 1000; Draw each 5 μ l of reference substance solution and need testing solution respectively, inject chromatograph of liquid, measure; In the test sample chromatograph, have the chromatographic peak consistent with the reference substance chromatographic retention.

Embodiments of the invention 15: the high-efficient liquid phase chromatogram process measuring method of naringin content in the tablet

Getting children's, to like the food effervescent formulation an amount of, accurate claims surely, adds the methanol supersound extraction, filters, and gets subsequent filtrate and regulate concentration, as need testing solution; The naringin reference substance that is dried to constant weight of learning from else's experience is an amount of, accurately claims surely, adds methanol and makes reference substance solution; With the dialkyl silane bonded silica gel is filler; Acetonitrile-water-phosphoric acid (50: 50: 0.5) is mobile phase; The detection wavelength is 255nm, and number of theoretical plate calculates by the naringin peak should be not less than 1000; Draw reference substance solution and need testing solution 10 μ l respectively, inject chromatograph of liquid, measure, promptly; Children's to be measured likes food effervescent formulation content limit and should be: one to five years old child once likes the food effervescent formulation with children's and contains Fructus Aurantii with naringin (C ₂₇H ₃₂O ₁₄) meter, must not be less than 1.0mg.

Embodiments of the invention 16: the high-efficient liquid phase chromatogram process measuring method of naringin content in the preparation

Getting children's, to like the food effervescent formulation an amount of, accurate claims surely, puts in the apparatus,Soxhlet's, it is an amount of to add ethanol, and reflux 4 hours is in the extracting solution dislocation evaporating dish, add an amount of washing container of methanol, merge extractive liquid, and washing liquid, evaporate to dryness, residue adds methanol and dissolves in right amount and be transferred in the 25ml measuring bottle, add methanol and be diluted to scale, shake up, filter, filter with microporous filter membrane, get subsequent filtrate as need testing solution; The 60 ℃ of drying under reduced pressure of learning from else's experience are an amount of to the naringin reference substance of constant weight, and accurate the title decides, and adds ethanol and makes the solution that every 1ml contains 50 μ g, promptly; With octadecylsilane chemically bonded silica is filler; Methanol-water-glacial acetic acid (35: 65: 0.4) is a mobile phase; The detection wavelength is 283nm, and number of theoretical plate calculates by the naringin peak should be not less than 1000; Accurate respectively reference substance solution and each 5 μ l of need testing solution of drawing inject chromatograph of liquid, measure, promptly; Children's to be measured likes food effervescent formulation content limit and should be: one to five years old child once likes the food effervescent formulation with children's and contains Fructus Aurantii with naringin (C ₂₇H ₃₂O ₁₄) meter, must not be less than 2.0mg.

Embodiments of the invention 17: the tlc determination method of ursolic acid content in the drop pill

It is an amount of that children's likes the food effervescent formulation, accurate claims surely, adds the acetone reflux, extract,, the extracting solution evaporate to dryness, and residue soaks with petroleum ether, and the petroleum ether that inclines, residue add that methanol-chloroform (1: 1) mixed liquor is an amount of, and slight fever makes dissolving, and standardize solution shakes up, as need testing solution; It is an amount of that precision takes by weighing the ursolic acid reference substance in addition, adds methanol and make reference substance solution; Test according to thin layer chromatography (an appendix VI of Chinese Pharmacopoeia version in 2005 B), the accurate need testing solution 5 μ l that draw, the cross point is on same silica gel g thin-layer plate respectively, with normal hexane-chloroform-Ethyl formate-acetic acid (5: 5: 1: 0.1) be developing solvent, launch, take out, dry, spray is with ethanol solution of sulfuric acid, and it is clear to be heated to the speckle colour developing, scans according to thin layer chromatography (an appendix VI of Chinese Pharmacopoeia version in 2005 B thin layer chromatography scanning), wavelength: λ s=620nm, measure test sample trap integrated value and reference substance trap integrated value, calculate, promptly; Children's to be measured likes food effervescent formulation content limit and should be: one to five years old child once likes the food effervescent formulation with children's and contains Fructus Crataegi with ursolic acid (C ₃₀H ₄₈O ₃) meter, must not be less than 0.5mg.

Embodiments of the invention 18: the tlc determination method of ursolic acid content in the granule

It is an amount of that children's likes the food effervescent granule, and accurate the title decides, and puts in the apparatus,Soxhlet's, it is an amount of to add diethyl ether, reflux 6 hours, and extracting solution reclaims ether to doing, residue soaks 2 times with petroleum ether, each 5ml (soaking 2 minutes), and petroleum ether inclines, it is an amount of that residue adds dehydrated alcohol, slight fever makes dissolving, is transferred in the 5ml measuring bottle, and is diluted to scale, shake up, as need testing solution; It is an amount of that precision takes by weighing the ursolic acid reference substance in addition, adds dehydrated alcohol and make reference substance solution; Test according to thin layer chromatography (an appendix VI of Chinese Pharmacopoeia version in 2005 B), accurate each the 10 μ l of need testing solution that draw, the cross point is on same silica gel g thin-layer plate respectively, with cyclohexane extraction-chloroform-ethyl acetate-acetic acid (20: 1: 8: 0.1) be developing solvent, launch, take out, dry, spray is with 10% ethanol solution of sulfuric acid, it is clear to be heated to speckle colour developing at 110 ℃, takes out, and covers onesize glass plate on lamellae, use immobilization with adhesive tape on every side, scan wavelength according to thin layer chromatography (an appendix VI of Chinese Pharmacopoeia version in 2005 B thin layer chromatography scanning): λ s=530nm, measure test sample trap integrated value and reference substance trap integrated value, calculate, promptly; Children's to be measured likes food effervescent formulation content limit and should be: one to five years old child once likes the food effervescent formulation with children's and contains Fructus Crataegi with ursolic acid (C ₃₀H ₄₈O ₃) meter, must not be less than 1.0mg.

Embodiments of the invention 19: the discrimination method of total flavones

Get this product powder 10g, add water 50ml and make dissolving, filter, filtrate adds ethyl acetate 20ml, and jolting is extracted, and gets ethyl acetate liquid, and evaporate to dryness, residue add methanol 2ml makes dissolving, filters, and filtrate adds that magnesium powder is a small amount of to drip with the salt acid number, apparent cherry red.

Embodiments of the invention 20: the assay method of ursolic acid content in the preparation

It is an amount of that children's likes the food effervescent formulation, and accurate the title decides, and puts in the apparatus,Soxhlet's, it is an amount of to add diethyl ether, reflux 6 hours, and extracting solution reclaims ether to doing, residue soaks 2 times with petroleum ether (30～60 ℃), each 5ml (soaking 2 minutes), and petroleum ether inclines, it is an amount of that residue adds dehydrated alcohol-chloroform (3: 2) mixed liquor, slight fever makes dissolving, is transferred in the 5ml measuring bottle, and is diluted to scale, shake up, as need testing solution; It is an amount of that precision takes by weighing the ursolic acid reference substance in addition, adds dehydrated alcohol and make the solution that every 1ml contains 0.5mg, in contrast product solution; Accurate need testing solution 5 μ l, reference substance solution 4 μ l and the 8 μ l of drawing, the cross point is on same silica gel g thin-layer plate respectively, with cyclohexane extraction-chloroform-ethyl acetate-formic acid (20: 5: 8: 0.1) be developing solvent, launch, take out, dry, spray is with 10% ethanol solution of sulfuric acid, and it is clear to be heated to speckle colour developing at 110 ℃, take out, on lamellae, cover onesize glass plate, use immobilization with adhesive tape on every side, scan according to thin layer chromatography scanning, wavelength: λ s=530nm, measure test sample trap integrated value and reference substance trap integrated value, calculate, promptly; Children's to be measured likes food effervescent formulation content limit and should be: one to five years old child once likes the food effervescent formulation with children's and contains Fructus Crataegi with ursolic acid (C ₃₀H ₄₈O ₃) meter, must not be less than 1.0mg.

Embodiments of the invention 21: the thin layer chromatography discrimination method of the Rhizoma Atractylodis Macrocephalae in the preparation

Getting children's, to like the food preparation an amount of, and the jolting that adds diethyl ether is extracted, and ether solution volatilizes, and residue adds methanol 1ml makes dissolving, as need testing solution; Other gets Rhizoma Atractylodis Macrocephalae control medicinal material, decocts with water, and filters, and filtrate is extracted with the ether jolting, and ether solution volatilizes, and residue adds methanol makes dissolving, in contrast medical material solution; Drawing each 10 μ l of above-mentioned two kinds of solution, put respectively on same silica gel g thin-layer plate, is developing solvent with chloroform-acetone-formic acid (9.5: 0.5: 0.06), launches, and takes out, and dries, and puts under the ultra-violet lamp (365nm) and inspects; In the test sample chromatograph, with the corresponding position of control medicinal material chromatograph on, show the fluorescence speckle of same color.

Embodiments of the invention 22: the thin layer chromatography discrimination method of Fructus Crataegi, ursolic acid in the preparation

Get children's like the food preparation an amount of, the 30ml that adds diethyl ether, supersound process 10 minutes is put cold, filter, the filtrate evaporate to dryness, residue soaks 2 times with petroleum ether (30～60 ℃), each 5ml (soaking half a minute approximately), the petroleum ether that inclines, residue adds methanol 1ml makes dissolving, as need testing solution; Other gets Fructus Crataegi control medicinal material 1g, and it is an amount of to add water, decocts about 30 minutes, filters, and the filtrate 30ml that adds diethyl ether extracts, and divides and gets ether solution, volatilizes, and residue adds methanol 1ml makes dissolving, in contrast medical material solution; Get the ursolic acid reference substance again, add methanol and make the solution that every 1ml contains 1mg, in contrast product solution; Draw each 5 μ l of above-mentioned need testing solution and contrast solution, put respectively in same be on the silica gel g thin-layer plate of adhesive with the sodium carboxymethyl cellulose, with chloroform-acetone (13: 1) is developing solvent, launch, take out, dry, spray is with 10% ethanol solution of sulfuric acid, and it is clear to be heated to the speckle colour developing at 105 ℃; In the test sample chromatograph, with contrast chromatograph corresponding position on, show the speckle of same color.

Embodiments of the invention 23: the thin layer chromatography discrimination method of Fructus Hordei Germinatus in the preparation

Get children's like the food preparation an amount of, add dehydrated alcohol 30ml, supersound process 40 minutes, filter, filtrate adds 50% potassium hydroxide solution 1ml, reflux 20 minutes, cooling rapidly, in the dislocation separatory funnel, water 20ml washing container, washing liquid is incorporated in the separatory funnel, extracts 2 times with petroleum ether (30～60 ℃), each 20ml, divide and get petroleum ether layer, volatilize, residue adds ethyl acetate 1ml makes dissolving, as need testing solution; Other gets Fructus Hordei Germinatus control medicinal material 5g, shines medical material solution in pairs with legal system; Drawing each 10 μ l of above-mentioned two kinds of solution, put respectively on same silica gel g thin-layer plate, is developing solvent with benzene-chloroform (1: 1), launch, take out, dry, spray is with 50% alcoholic solution of 15% nitric acid, and it is clear to be heated to the speckle colour developing at 100 ℃, puts under the ultra-violet lamp (365nm) and inspects; In the test sample chromatograph, with the corresponding position of control medicinal material chromatograph on, show the fluorescence speckle of same color.

Embodiments of the invention 24: the thin layer chromatography discrimination method of Fructus Aurantii in the preparation

Getting children's, to like the food preparation an amount of, adds ethanol 10ml, and reflux 20 minutes filters, and filtrate is waved to 1ml, as need testing solution; Other gets Fructus Aurantii control medicinal material 2g, shines medical material solution in pairs with legal system; Drawing each 10 μ l of above-mentioned two kinds of solution, put respectively on same silica gel g thin-layer plate, is developing solvent with cyclohexane extraction-chloroform-ethyl acetate (20: 5: 5), launches, and takes out, and dries, and puts under the ultra-violet lamp (365mm) and inspects; In the test sample chromatograph, with the corresponding position of control medicinal material chromatograph on, show the fluorescence speckle of same color.

Embodiments of the invention 25: the high performance liquid chromatography discrimination method of naringin in the preparation

It is an amount of that children's likes the food preparation, puts in the apparatus,Soxhlet's, and it is an amount of to add methanol, reflux 4 hours, and the extracting solution evaporate to dryness, residue adds dissolve with methanol, filters, and gets subsequent filtrate as need testing solution; It is an amount of to get the naringin reference substance, adds methanol and makes reference substance solution; With octadecylsilane chemically bonded silica is filler; Methanol-water-glacial acetic acid (35: 65: 0.4) is a mobile phase; The detection wavelength is 283nm, and number of theoretical plate calculates by the naringin peak should be not less than 1000; Draw each 10 μ l of reference substance solution and need testing solution respectively, inject chromatograph of liquid, measure; In the test sample chromatograph, have the chromatographic peak consistent with the reference substance chromatographic retention.

Claims (10)

1. pharmaceutical preparation for the treatment of infantile anorexia is characterized in that: it is made into effervescent tablet, injection, powder pin, freeze-dried powder, gel, dispersible tablet, capsule, soft capsule, microcapsule, granule, pill, micropill, powder, drop pill, slow releasing preparation, controlled release preparation, gel, oral liquid, soft extract, extractum or membrane by Massa Medicata Fermentata 385g, Fructus Aurantii 195g, Rhizoma Atractylodis Macrocephalae 195g, Fructus Crataegi 295g, Fructus Oryzae Germinatus 960g, Fructus Hordei Germinatus 960g or with their extract of corresponding weight portion.

2. according to the pharmaceutical preparation of the described treatment infantile anorexia of claim 1, it is characterized in that: described preparation is effervescent tablet, oral liquid, gel or pellet.

3. the preparation method of the pharmaceutical preparation of treatment infantile anorexia as claimed in claim 1 or 2 is characterized in that: get Massa Medicata Fermentata, Fructus Hordei Germinatus, Fructus Oryzae Germinatus and add behind the water boil 80～90 ℃ of dippings 2 hours, left standstill 4 hours, filter filtrate for later use; Other gets the Rhizoma Atractylodis Macrocephalae, Fructus Aurantii, Fructus Crataegi and decocts with water secondary, and each 1.5 hours, collecting decoction left standstill, filter, filtrate and above-mentioned filtrate merge, and are concentrated into relative density and are about 1.20 clear paste, drying under reduced pressure, pulverize, the cream powder that sieves is made different preparations then respectively.

4. according to the preparation method of the pharmaceutical preparation of the described treatment infantile anorexia of claim 3, it is characterized in that: the effervescent tablet in the described preparation prepares like this: get Massa Medicata Fermentata, Fructus Hordei Germinatus, Fructus Oryzae Germinatus and add behind the water boil 80～90 ℃ of dippings 2 hours, left standstill 4 hours, and filtered filtrate for later use; Other gets the Rhizoma Atractylodis Macrocephalae, Fructus Aurantii, Fructus Crataegi and decocts with water secondary, and each 1.5 hours, collecting decoction, leave standstill, filter, filtrate and above-mentioned filtrate merge, relative density is 1.20 clear paste when being concentrated into 50 ℃, and drying under reduced pressure is pulverized, the cream powder that sieves, the cream powder adds sodium bicarbonate 2500g, citric acid 600g, fumaric acid 300g, steviosin 10g, magnesium stearate 25g, lactose 550g, mix homogeneously, tabletting, pressure is at 4.0～5.0kg/cm2, promptly.

5. according to the preparation method of the pharmaceutical preparation of the described treatment infantile anorexia of claim 3, it is characterized in that: the gel in the described preparation prepares like this: get Massa Medicata Fermentata, Fructus Hordei Germinatus, Fructus Oryzae Germinatus and add behind the water boil 80～90 ℃ of dippings 2 hours, left standstill 4 hours, and filtered filtrate for later use; Other gets the Rhizoma Atractylodis Macrocephalae, Fructus Aurantii, Fructus Crataegi and decocts with water secondary, and each 1.5 hours, collecting decoction left standstill, and filters filtrate and the merging of above-mentioned filtrate; Konjac glucomannan, K-carrageenan are pressed 1: 3 part by weight mix homogeneously, under stirring condition, 0.8% mixed-matrix is put into cold water, make it to disperse, soak 20～30min, make the abundant water absorption and swelling of substrate, stir then, heat, filter, filter the back and under condition of stirring, add herbal extract, mix homogeneously joins rapidly in the disinfecting container, seals, sterilization is cooled to about 30 ℃ condensation rapidly, drying, promptly

6. the method for quality control of the pharmaceutical preparation of treatment infantile anorexia as claimed in claim 1 or 2 is characterized in that: this method comprises following all or part of content:

(1) the differential test method of the Rhizoma Atractylodis Macrocephalae, Fructus Crataegi, ursolic acid, Fructus Hordei Germinatus, Fructus Aurantii, naringin, flavones ingredient;

(2) naringin, content of ursolic acid method of testing.

7. according to the method for quality control of the pharmaceutical preparation of the described treatment infantile anorexia of claim 6, it is characterized in that: the discrimination method of described preparation comprises following all or part of content:

A. the thin layer chromatography discrimination method of the Rhizoma Atractylodis Macrocephalae in the preparation:

Getting children's, to like the food preparation an amount of, adds diethyl ether or chloroform or ethyl acetate jolting are extracted, and extracting solution volatilizes, and residue adds chloroform or ethyl acetate or methanol or ethanol makes dissolving, as need testing solution; Other gets Rhizoma Atractylodis Macrocephalae control medicinal material, decocts with water, and filters, and filtrate adds diethyl ether or chloroform or ethyl acetate jolting are extracted, and extracting solution volatilizes, and residue adds chloroform or ethyl acetate or methanol or ethanol makes dissolving, in contrast medical material solution; Draw each 1～30 μ l of above-mentioned two kinds of solution, put respectively on same silica gel G or silica gel H or silica GF254 lamellae, with chloroform or dichloromethane-acetone or butanone-formic acid or acetic acid=1～10: be developing solvent at 0～2: 0～1, launch, take out, dry, put under the ultra-violet lamp and inspect; In the test sample chromatograph, with the corresponding position of control medicinal material chromatograph on, show the fluorescence speckle of same color;

B. one or both thin layer chromatography discrimination method in Fructus Crataegi, the ursolic acid in the preparation:

Get children's like the food preparation an amount of, add diethyl ether or chloroform or ethyl acetate or ethanol or methanol or acetone extraction the extracting solution evaporate to dryness, residue soaks with petroleum ether, the petroleum ether that inclines, residue add methanol or chloroform or ethyl acetate or ethanol makes dissolving, as need testing solution; Other gets the Fructus Crataegi control medicinal material, decocts with water, and decocting liquid adds diethyl ether or chloroform or ethyl acetate extraction, and extracting solution volatilizes, and residue adds methanol or chloroform or ethyl acetate or ethanol makes dissolving, in contrast medical material solution; Get the ursolic acid reference substance again, add methanol or chloroform or ethyl acetate or ethanol and make dissolving, in contrast product solution; Draw one or both each 1～30 μ l in above-mentioned need testing solution and the contrast solution, put respectively on same silica gel G or silica gel H or silica GF254 lamellae, with chloroform or dichloromethane-acetone or butanone=1～10: 0～1 is developing solvent, or with benzene or toluene-ethyl acetate or butyl acetate or Ethyl formate-or formic acid or acetic acid=1～20: be developing solvent at 1～4: 0～2, launch, take out, dry, spray is with ethanol solution of sulfuric acid, and it is clear to be heated to the speckle colour developing; In the test sample chromatograph, with contrast chromatograph corresponding position on, show the speckle of same color;

C. the thin layer chromatography discrimination method of Fructus Hordei Germinatus in the preparation:

Get children's like the food preparation an amount of, add dehydrated alcohol or methanol extraction, extracting solution adds strong base solution, reflux, cooling rapidly, in the dislocation separatory funnel, it is an amount of to add water, with petroleum ether or ether extraction, extracting solution volatilizes, and residue adds ethyl acetate or chloroform makes dissolving, as need testing solution; Other gets the Fructus Hordei Germinatus control medicinal material, shines medical material solution in pairs with legal system; Draw each 1～30 μ l of above-mentioned two kinds of solution, put respectively on same silica gel G or silica gel H or silica GF254 lamellae, with benzene or toluene or dimethylbenzene-chloroform or dichloromethane=0～1: 0～1 is developing solvent, launch, take out, dry, spray is to contain the alcoholic solution of nitric acid, it is clear to be heated to the speckle colour developing, puts under the ultra-violet lamp and inspects; In the test sample chromatograph, with the corresponding position of control medicinal material chromatograph on, show the fluorescence speckle of same color;

D. the thin layer chromatography discrimination method of Fructus Aurantii in the preparation:

Getting children's, to like the food preparation an amount of, adds ethanol or methanol or acetone extraction, and extracting solution concentrates, as need testing solution; Other gets the Fructus Aurantii control medicinal material, shines medical material solution in pairs with legal system; Draw each 1～30 μ l of above-mentioned two kinds of solution, put respectively on same silica gel G or silica gel H or silica GF254 lamellae, with cyclohexane extraction or normal hexane-chloroform or dichloromethane-ethyl acetate or butyl acetate or Ethyl formate=5～20: be developing solvent at 1～5: 1～5, launch, take out, dry, put under the ultra-violet lamp and inspect; In the test sample chromatograph, with the corresponding position of control medicinal material chromatograph on, show the fluorescence speckle of same color;

E. the high performance liquid chromatography discrimination method of naringin in the preparation:

It is an amount of that children's likes the food preparation, adds ethanol or methanol extraction, and extracting solution filters, as need testing solution; It is an amount of to get the naringin reference substance, adds ethanol or methanol is made reference substance solution; With octadecylsilane chemically bonded silica or eight alkyl silane bonded silica gels or dialkyl silane bonded silica gel is filler; Methanol or acetonitrile-water-acetic acid or formic acid or phosphoric acid=5～35: 15～65: 0～5 is mobile phase; The detection wavelength is one or several among 190～140nm, and number of theoretical plate calculates by the naringin peak should be not less than 1000; Draw reference substance solution and need testing solution 1～30 μ l respectively, inject chromatograph of liquid, measure; In the test sample chromatograph, have the chromatographic peak consistent with the reference substance chromatographic retention;

F. the chemical property discrimination method of flavones ingredient in the preparation:

It is an amount of that children's likes the food preparation, adds water and make dissolving, filters, and filtrate adds ethyl acetate, and jolting is extracted, and gets ethyl acetate liquid, and evaporate to dryness, residue add methanol makes dissolving, filters, and filtrate adds that magnesium powder is a small amount of to drip with the salt acid number, apparent cherry red.

8. according to the method for quality control of the pharmaceutical preparation of the described treatment infantile anorexia of claim 7, it is characterized in that: the discrimination method of described preparation comprises following all or part of content:

A. the thin layer chromatography discrimination method of the Rhizoma Atractylodis Macrocephalae in the preparation:

Getting children's, to like the food preparation an amount of, and the jolting that adds diethyl ether is extracted, and ether solution volatilizes, and residue adds methanol 1ml makes dissolving, as need testing solution; Other gets Rhizoma Atractylodis Macrocephalae control medicinal material, decocts with water, and filters, and filtrate is extracted with the ether jolting, and ether solution volatilizes, and residue adds methanol makes dissolving, in contrast medical material solution; Drawing each 10 μ l of above-mentioned two kinds of solution, put respectively on same silica gel g thin-layer plate, be developing solvent with chloroform-acetone-formic acid=9.5: 0.5: 0.06, launches, and taking-up is dried, and puts under the 365nm ultra-violet lamp and inspects; In the test sample chromatograph, with the corresponding position of control medicinal material chromatograph on, show the fluorescence speckle of same color;

B. one or both thin layer chromatography discrimination method in Fructus Crataegi, the ursolic acid in the preparation:

Get children's like the food preparation an amount of, the 30ml that adds diethyl ether, supersound process 10 minutes is put cold, filter petroleum ether=30～60 ℃ immersion 2 times of filtrate evaporate to dryness, residue, each 5ml, soak half a minute approximately, the petroleum ether that inclines, residue add methanol 1ml makes dissolving, as need testing solution; Other gets Fructus Crataegi control medicinal material 1g, and it is an amount of to add water, decocts about 30 minutes, filters, and the filtrate 30ml that adds diethyl ether extracts, and divides and gets ether solution, volatilizes, and residue adds methanol 1ml makes dissolving, in contrast medical material solution; Get the ursolic acid reference substance again, add methanol and make the solution that every 1ml contains 1mg, in contrast product solution; Draw one or both each the 5 μ l in above-mentioned need testing solution and the contrast solution, put respectively in same be on the silica gel g thin-layer plate of adhesive with the sodium carboxymethyl cellulose, with chloroform-acetone=13: 1 was developing solvent, launch, take out, dry, spray is with 10% ethanol solution of sulfuric acid, and it is clear to be heated to the speckle colour developing at 105 ℃; In the test sample chromatograph, with contrast chromatograph corresponding position on, show the speckle of same color;

C. the thin layer chromatography discrimination method of Fructus Hordei Germinatus in the preparation:

Get children's like the food preparation an amount of, add dehydrated alcohol 30ml, supersound process 40 minutes, filter, filtrate adds 50% potassium hydroxide solution 1ml, reflux 20 minutes, cooling rapidly, in the dislocation separatory funnel, water 20ml washing container, washing liquid is incorporated in the separatory funnel, with petroleum ether=30～60 ℃ extraction 2 times, each 20ml, divide and get petroleum ether layer, volatilize, residue adds ethyl acetate 1ml makes dissolving, as need testing solution; Other gets Fructus Hordei Germinatus control medicinal material 5g, shines medical material solution in pairs with legal system; Drawing each 10 μ l of above-mentioned two kinds of solution, put respectively on same silica gel g thin-layer plate, is developing solvent with benzene-chloroform=1: 1, launch, take out, dry, spray is with 50% alcoholic solution of 15% nitric acid, and it is clear to be heated to the speckle colour developing at 100 ℃, puts under the 365nm ultra-violet lamp and inspects; In the test sample chromatograph, with the corresponding position of control medicinal material chromatograph on, show the fluorescence speckle of same color;

D. the thin layer chromatography discrimination method of Fructus Aurantii in the preparation:

Getting children's, to like the food preparation an amount of, adds ethanol 10ml, and reflux 20 minutes filters, and filtrate is waved to 1ml, as need testing solution; Other gets Fructus Aurantii control medicinal material 2g, shines medical material solution in pairs with legal system; Drawing each 10 μ l of above-mentioned two kinds of solution, put respectively on same silica gel g thin-layer plate, be developing solvent with cyclohexane extraction-chloroform-ethyl acetate=20: 5: 5, launches, and taking-up is dried, and puts under the 365mm ultra-violet lamp and inspects; In the test sample chromatograph, with the corresponding position of control medicinal material chromatograph on, show the fluorescence speckle of same color;

E. the high performance liquid chromatography discrimination method of naringin in the preparation:

It is an amount of that children's likes the food preparation, puts in the apparatus,Soxhlet's, and it is an amount of to add methanol, reflux 4 hours, and the extracting solution evaporate to dryness, residue adds dissolve with methanol, filters, and gets subsequent filtrate as need testing solution; It is an amount of to get the naringin reference substance, adds methanol and makes reference substance solution; With octadecylsilane chemically bonded silica is filler; Methanol-water-glacial acetic acid=35: 65: 0.4 is a mobile phase; The detection wavelength is 283nm, and number of theoretical plate calculates by the naringin peak should be not less than 1000; Draw each 10 μ l of reference substance solution and need testing solution respectively, inject chromatograph of liquid, measure; In the test sample chromatograph, have the chromatographic peak consistent with the reference substance chromatographic retention;

F. the chemical property discrimination method of flavones ingredient in the preparation:

It is an amount of that children's likes the food preparation, adds water 50ml and make dissolving, filters, and filtrate adds ethyl acetate 20ml, and jolting is extracted, and gets ethyl acetate liquid, and evaporate to dryness, residue add methanol 2ml makes dissolving, filters, and filtrate adds that magnesium powder is a small amount of to drip with the salt acid number, apparent cherry red.

9. according to the method for quality control of the pharmaceutical preparation of the described treatment infantile anorexia of claim 6, it is characterized in that: the content assaying method of described preparation comprises following all or part of content:

A. the high-efficient liquid phase chromatogram process measuring method of naringin content in the preparation:

Getting children's, to like the food preparation an amount of, accurate claims surely, adds methanol or ethanol extraction, filters, and gets subsequent filtrate and regulate concentration, as need testing solution; The naringin reference substance that is dried to constant weight of learning from else's experience is an amount of, accurately claims surely, adds methanol or ethanol is made reference substance solution; With octadecylsilane chemically bonded silica or eight alkyl silane bonded silica gels or dialkyl silane bonded silica gel is filler; Methanol or acetonitrile-water-acetic acid or formic acid or phosphoric acid=5～35: 15～65: 0～5 is mobile phase; The detection wavelength is one or several among 190～140nm, and number of theoretical plate calculates by the naringin peak should be not less than 1000; Draw reference substance solution and need testing solution 1～30 μ l respectively, inject chromatograph of liquid, measure, promptly; Children's to be measured likes food formulation content limit and should be: one to five years old child once likes the food preparation with children's and contains Fructus Aurantii in naringin C27H32O14, must not be less than 1.0mg;

B. the assay method of ursolic acid content in the preparation:

It is an amount of that children's likes the food preparation, the accurate title, decide, add diethyl ether or chloroform or ethyl acetate or acetone or alcohol or methanol extraction, the extracting solution evaporate to dryness, residue soaks with petroleum ether, petroleum ether inclines, residue adds dehydrated alcohol or methanol-chloroform=0～3: 0～2 mixed liquor is an amount of, and slight fever makes dissolving, standardize solution, shake up, as need testing solution; It is an amount of that in addition precision takes by weighing the ursolic acid reference substance, adds dehydrated alcohol or methanol is made reference substance solution; Accurate need testing solution 1～30 μ l that draws, respectively the cross point is on same silica gel g thin-layer plate, and with cyclohexane extraction or normal hexane-chloroform or dichloromethane-ethyl acetate or butyl acetate or Ethyl formate-formic acid or acetic acid=5～20: be developing solvent at 1～5: 1～8: 0～1, expansion, take out, dry, spray is with ethanol solution of sulfuric acid, and it is clear to be heated to speckle colour developing, scan according to thin layer chromatography scanning, measure test sample trap integrated value and reference substance trap integrated value, calculate, promptly; Children's to be measured likes food formulation content limit and should be: one to five years old child once likes the food preparation with children's and contains Fructus Crataegi in ursolic acid C30H48O3, must not be less than 0.5mg.

10. according to the method for quality control of the pharmaceutical preparation of the described treatment infantile anorexia of claim 9, it is characterized in that: the content assaying method of described preparation comprises following all or part of content:

A. the high-efficient liquid phase chromatogram process measuring method of naringin content in the preparation:

Getting children's, to like the food preparation an amount of, accurate claims surely, puts in the apparatus,Soxhlet's, it is an amount of to add methanol, and reflux 4 hours is in the extracting solution dislocation evaporating dish, add an amount of washing container of methanol, merge extractive liquid, and washing liquid, evaporate to dryness, residue adds methanol and dissolves in right amount and be transferred in the 25ml measuring bottle, add methanol and be diluted to scale, shake up, filter, filter with 0.45 μ m microporous filter membrane, get subsequent filtrate as need testing solution; The 60 ℃ of drying under reduced pressure of learning from else's experience are an amount of to the naringin reference substance of constant weight, and accurate the title decides, and adds methanol and makes the solution that every 1ml contains 50 μ g, promptly; With octadecylsilane chemically bonded silica is filler; Methanol-water-glacial acetic acid=35: 65: 0.4 is a mobile phase; The detection wavelength is 283nm, and number of theoretical plate calculates by the naringin peak should be not less than 1000; Accurate respectively reference substance solution and each 10 μ l of need testing solution of drawing inject chromatograph of liquid, measure, promptly; Children's to be measured likes food formulation content limit and should be: one to five years old child once likes the food preparation with children's and contains Fructus Aurantii in naringin C27H32O14, must not be less than 2.0mg;

B. the assay method of ursolic acid content in the preparation:

It is an amount of that children's likes the food preparation, and accurate the title decides, and puts in the apparatus,Soxhlet's, it is an amount of to add diethyl ether, reflux 6 hours, and extracting solution reclaims ether to doing, residue soaks 2 times with 30～60 ℃ of petroleum ether, and each 5ml soaked 2 minutes, the petroleum ether that inclines, residue add that dehydrated alcohol-chloroform=mixed liquor was an amount of in 3: 2, and slight fever makes dissolving, be transferred in the 5ml measuring bottle, and be diluted to scale, shake up, as need testing solution; It is an amount of that precision takes by weighing the ursolic acid reference substance in addition, adds dehydrated alcohol and make the solution that every 1ml contains 0.5mg, in contrast product solution; Accurate need testing solution 5 μ l, reference substance solution 4 μ l and the 8 μ l of drawing, the cross point is on same silica gel g thin-layer plate respectively, with cyclohexane extraction-chloroform-ethyl acetate-formic acid=20: 5: 8: 0.1 was developing solvent, launch, take out, dry, spray is with 10% ethanol solution of sulfuric acid, and it is clear to be heated to speckle colour developing at 110 ℃, take out, on lamellae, cover onesize glass plate, use immobilization with adhesive tape on every side, scan according to thin layer chromatography scanning, wavelength: λ s=530nm, measure test sample trap integrated value and reference substance trap integrated value, calculate, promptly; Children's to be measured likes food formulation content limit and should be: one to five years old child once likes the food preparation with children's and contains Fructus Crataegi in ursolic acid C30H48O3, must not be less than 1.0mg.